242 lines
17 KiB
XML
242 lines
17 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.77.59852" ID-GBIF-Dataset="78bab002-8c3e-4548-8377-cb082fa11d13" ID-PMC="PMC7819953" ID-Pensoft-Pub="1314-4049-77-65" ID-Pensoft-UUID="5D8D5A6329A4584ABC11C425E4B0E47A" ID-PubMed="33519269" ModsDocID="1314-4049-77-65" checkinTime="1610662561303" checkinUser="pensoft" docAuthor="Sun, Wenxiu, Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Li, Zhuang" docDate="2021" docId="B3D78854073C5B4EBEDA51AE211D1FC1" docLanguage="en" docName="MycoKeys 77: 65-95" docOrigin="MycoKeys 77" docPubDate="2021-01-14" docSource="http://dx.doi.org/10.3897/mycokeys.77.59852" docTitle="Diaporthe melastomatis S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li 2021, sp. nov." docType="treatment" docVersion="4" id="5D8D5A6329A4584ABC11C425E4B0E47A" lastPageNumber="65" masterDocId="5D8D5A6329A4584ABC11C425E4B0E47A" masterDocTitle="Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species" masterLastPageNumber="95" masterPageNumber="65" pageNumber="65" updateTime="1668136608950" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sun, Wenxiu</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Huang, Shengting</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xia, Jiwen</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7436-7249</mods:nameIdentifier>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Xiuguo</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Li, Zhuang</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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<mods:nameIdentifier type="email">junwuxue@126.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-01-14</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>77</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>65</mods:start>
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<mods:end>95</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.77.59852</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.77.59852</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-77-65</mods:identifier>
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<mods:identifier type="Pensoft-UUID">5D8D5A6329A4584ABC11C425E4B0E47A</mods:identifier>
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<treatment ID-GBIF-Taxon="176584076" LSID="urn:lsid:plazi:treatment:B3D78854073C5B4EBEDA51AE211D1FC1" httpUri="http://treatment.plazi.org/id/B3D78854073C5B4EBEDA51AE211D1FC1" lastPageNumber="65" pageId="0" pageNumber="65">
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<subSubSection pageId="0" pageNumber="65" type="nomenclature">
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName LSID="B3D78854-073C-5B4E-BEDA-51AE211D1FC1" authority="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe melastomatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="melastomatis" status="sp. nov.">Diaporthe melastomatis S.T. Huang, J.W. Xia, X.G. Zhang & Z. Li</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="65">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 8" captionStartId="F9" captionText="Figure 8. Diaporthe melastomatis (SAUCC 194.55) a branch with leaves of host plant b, c surface (b) and reverse (c) sides of colony after incubation for 15 days on PDA d conidiomata e, f conidiophores and conidiogenous cells g beta conidia h, i, k alpha conidia and beta conidia j alpha conidia. Scale bars: 10 μm (e-k)." figureDoi="10.3897/mycokeys.77.59852.figure8" httpUri="https://binary.pensoft.net/fig/497511" pageId="0" pageNumber="65">Figure 8</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="etymology">
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<paragraph pageId="0" pageNumber="65">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Named after the host
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<taxonomicName class="Dicotyledoneae" family="Melastomataceae" genus="Melastoma" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Melastoma malabathricum" order="Myrtiflorae" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="malabathricum">
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<emphasis italics="true" pageId="0" pageNumber="65">Melastoma malabathricum</emphasis>
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</taxonomicName>
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on which it was collected.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="diagnosis">
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<paragraph pageId="0" pageNumber="65">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe melastomatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="melastomatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe melastomatis</emphasis>
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</taxonomicName>
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differs from
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<taxonomicName lsidName="D. parapterocarpi" pageId="0" pageNumber="65" rank="species" species="parapterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. parapterocarpi</emphasis>
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</taxonomicName>
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Crous in smaller
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<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
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and the types of conidia.
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</paragraph>
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<caption doi="10.3897/mycokeys.77.59852.figure8" httpUri="https://binary.pensoft.net/fig/497511" pageId="0" pageNumber="65" start="Figure 8" startId="F9">
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<paragraph pageId="0" pageNumber="65">
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<emphasis bold="true" pageId="0" pageNumber="65">Figure 8.</emphasis>
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe melastomatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="melastomatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe melastomatis</emphasis>
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</taxonomicName>
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(SAUCC194.55)
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<emphasis bold="true" pageId="0" pageNumber="65">a</emphasis>
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branch with leaves of host plant
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<emphasis bold="true" pageId="0" pageNumber="65">b, c</emphasis>
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surface (
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<emphasis bold="true" pageId="0" pageNumber="65">b</emphasis>
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) and reverse (
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<emphasis bold="true" pageId="0" pageNumber="65">c</emphasis>
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) sides of colony after incubation for 15 days on PDA
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<emphasis bold="true" pageId="0" pageNumber="65">d</emphasis>
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conidiomata
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<emphasis bold="true" pageId="0" pageNumber="65">e, f</emphasis>
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conidiophores and conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="65">g</emphasis>
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beta conidia
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<emphasis bold="true" pageId="0" pageNumber="65">h, i, k</emphasis>
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alpha conidia and beta conidia
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<emphasis bold="true" pageId="0" pageNumber="65">j</emphasis>
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alpha conidia. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="65">e-k</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="materials_examined">
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<paragraph pageId="0" pageNumber="65">Type.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on diseased leaves of
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<taxonomicName class="Dicotyledoneae" family="Melastomataceae" genus="Melastoma" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Melastoma malabathricum" order="Myrtiflorae" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="malabathricum">
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<emphasis italics="true" pageId="0" pageNumber="65">Melastoma malabathricum</emphasis>
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</taxonomicName>
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. 19 April 2019, S.T. Huang, HSAUP194.55, holotype, ex-holotype living culture, SAUCC194.55.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Description.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Asexual morph: Conidiomata pycnidial, subglobose to globose, black, erumpent, coated with white hyphae, thick-walled, yellowish spiral conidial cirrus exuded from ostioles. Conidiophores hyaline, smooth, septate, branched, densely aggregated, cylindric-clavate, straight to slightly sinuous, tapering towards the apex, 14.5-21.0
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<normalizedToken originalValue="×">x</normalizedToken>
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2.0-3.2
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<normalizedToken originalValue="μm">μm</normalizedToken>
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. Conidiogenous cells 9.5-13.0
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-2.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, cylindrical, guttulate, terminal, tapering towards the base. Alpha conidia, hyaline, smooth, aseptate, oblong ellipsoidal, 2-4 guttulate, apex subobtuse, base subtruncate, 5.5-8.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.7-2.5
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 6.8
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<normalizedToken originalValue="×">x</normalizedToken>
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2.1
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Beta conidia abundant in the culture, hyaline, aseptate, filiform, multi-guttulate, sigmoid to lunate, mostly curved through 90-180°, tapering towards both ends, 25.0-33.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.1-2.0
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(mean = 27.6
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<normalizedToken originalValue="×">x</normalizedToken>
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1.4
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20). Gamma conidia and sexual morph not observed.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="65">Pure culture was isolated by subbing hyphal tips growing from surface sterilized diseased material. Colonies on PDA cover the Petri diameter after incubation for 15 days in dark conditions at 25 °C, cottony and lobate with abundant aerial mycelium, hyphae white in the margin on surface side, with pale brown concentric ring of dense hyphae on reverse side.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="materials_examined">
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<paragraph pageId="0" pageNumber="65">Additional specimens examined.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, 19 April 2019, S.T. Huang. On diseased leaves of
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<taxonomicName class="Globothalamea" family="Millettiidae" genus="Millettia" higherTaxonomySource="GBIF,CoL" kingdom="Chromista" lsidName="Millettia reticulata" order="Rotaliida" pageId="0" pageNumber="65" phylum="Foraminifera" rank="species" species="reticulata">
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<emphasis italics="true" pageId="0" pageNumber="65">Millettia reticulata</emphasis>
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</taxonomicName>
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, HSAUP194.80 paratype, living culture SAUCC194.80; on infected leaves of
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<taxonomicName class="Dicotyledoneae" family="Theaceae" genus="Camellia" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Camellia sinensis" order="Guttiferales" pageId="0" pageNumber="65" phylum="Angiospermae" rank="species" species="sinensis">
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<emphasis italics="true" pageId="0" pageNumber="65">Camellia sinensis</emphasis>
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</taxonomicName>
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, HSAUP194.88 paratype, living culture SAUCC194.88.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="notes">
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<paragraph pageId="0" pageNumber="65">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe melastomatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="melastomatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe melastomatis</emphasis>
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</taxonomicName>
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is introduced based on the multi-locus phylogenetic analysis, with three isolates clustering separately in a well-supported clade (ML/BI = 100/1).
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe melastomatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="melastomatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe melastomatis</emphasis>
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</taxonomicName>
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is most closely related to
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<taxonomicName lsidName="D. parapterocarpi" pageId="0" pageNumber="65" rank="species" species="parapterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. parapterocarpi</emphasis>
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</taxonomicName>
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, but distinguished based on ITS and
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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loci from
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<taxonomicName lsidName="D. parapterocarpi" pageId="0" pageNumber="65" rank="species" species="parapterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. parapterocarpi</emphasis>
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</taxonomicName>
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by 32 nucleotides difference in the concatenated alignment, in which 20 are distinct in the ITS region, 12 in the
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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region. Morphologically,
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe melastomatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="melastomatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe melastomatis</emphasis>
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</taxonomicName>
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differs from
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<taxonomicName lsidName="D. parapterocarpi" pageId="0" pageNumber="65" rank="species" species="parapterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. parapterocarpi</emphasis>
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</taxonomicName>
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in its smaller alpha conidia (5.5-8.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.7-2.5 vs. 8.0-10.0
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<normalizedToken originalValue="×">x</normalizedToken>
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2.5-3.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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). Furthermore,
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe melastomatis" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="melastomatis">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe melastomatis</emphasis>
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</taxonomicName>
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can produce beta conidia, but
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<taxonomicName lsidName="D. parapterocarpi" pageId="0" pageNumber="65" rank="species" species="parapterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. parapterocarpi</emphasis>
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</taxonomicName>
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cannot (
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<bibRefCitation DOI="https://doi.org/10.3767/003158514X682395" author="Crous, PW" journalOrPublisher="Persoonia" pageId="0" pageNumber="65" pagination="184 - 306" refId="B8" refString="Crous, PW, Shivas, RG, Quaedvlieg, W, van der Bank, M, Zhang, Y, Summerell, BA, Guarro, J, Wingfield, MJ, Wood, AR, Alfenas, AC, 2014. Fungal planet description sheets: 214-280. Persoonia 32 (1): 184 - 306, DOI: https://doi.org/10.3767/003158514X682395" title="Fungal planet description sheets: 214 - 280." url="https://doi.org/10.3767/003158514X682395" volume="32" year="2014">Crous et al. 2014</bibRefCitation>
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).
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</paragraph>
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</subSubSection>
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</treatment>
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||
</document> |