244 lines
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244 lines
15 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.30.22887" ID-GBIF-Dataset="38da3978-f8ec-466a-b328-7069fb36e525" ID-PMC="PMC5904530" ID-Pensoft-Pub="1314-4049-30-61" ID-PubMed="29681733" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-30-61" ModsDocOrigin="MycoKeys 30" ModsDocTitle="Tuberaztecorum sp. nov., a truffle species from Mexico belonging to the Maculatum clade (Tuberaceae, Pezizales)" checkinTime="1519861739913" checkinUser="pensoft" docAuthor="Guevara-Guerrero, Gonzalo, Bonito, Gregory, Smith, Matthew E., Healy, Rosanne, II, Arthur C. Grupe, Cazares, Efren, Castellano, Michael A. & Trappe, James M." docDate="2018" docId="0BE3F571D06BB03C75A122E09492F6D4" docLanguage="en" docName="MycoKeys 30: 61-72" docOrigin="MycoKeys 30" docSource="http://dx.doi.org/10.3897/mycokeys.30.22887" docTitle="Tuber aztecorum Guevara, Bonito & Smith, sp. nov." docType="treatment" docVersion="4" lastPageNumber="65" masterDocId="3B51FFC72C413054FFBD0A6BFFDF3241" masterDocTitle="Tuberaztecorum sp. nov., a truffle species from Mexico belonging to the Maculatum clade (Tuberaceae, Pezizales)" masterLastPageNumber="72" masterPageNumber="61" pageNumber="63" updateTime="1668135906717" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Tuberaztecorum sp. nov., a truffle species from Mexico belonging to the Maculatum clade (Tuberaceae, Pezizales)</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Guevara-Guerrero, Gonzalo</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Bonito, Gregory</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Smith, Matthew E.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Healy, Rosanne</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>II, Arthur C. Grupe</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Cazares, Efren</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Castellano, Michael A.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Trappe, James M.</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2018</mods:date>
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<mods:detail type="volume">
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<mods:number>30</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>61</mods:start>
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<mods:end>72</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.30.22887</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.30.22887</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-30-61</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="141251045" LSID="urn:lsid:plazi:treatment:0BE3F571D06BB03C75A122E09492F6D4" httpUri="http://treatment.plazi.org/id/0BE3F571D06BB03C75A122E09492F6D4" lastPageId="4" lastPageNumber="65" pageId="2" pageNumber="63">
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<subSubSection pageId="2" pageNumber="63" type="nomenclature">
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<paragraph pageId="2" pageNumber="63">
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<taxonomicName LSID="MB819367" authority="Guevara, Bonito & Smith" class="Pezizomycetes" family="Tuberaceae" genus="Tuber" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tuber aztecorum" order="Pezizales" pageId="2" pageNumber="63" phylum="Ascomycota" rank="species" species="aztecorum">Tuber aztecorum Guevara, Bonito & Smith</taxonomicName>
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<taxonomicNameLabel pageId="2" pageNumber="63">sp. nov.</taxonomicNameLabel>
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Figs 1, 2
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<normalizedToken originalValue="A–I">A-I</normalizedToken>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="2" pageNumber="63" type="materials_examined">
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<paragraph pageId="2" pageNumber="63">Type.</paragraph>
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<paragraph pageId="2" pageNumber="63">
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MEXICO. State of Mexico, Toluca-Temascaltepec road, La Puerta, Parque Nacional Nevado de Toluca, 29 July 2010, Guevara 993 (ITCV [
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<normalizedToken originalValue="José">Jose</normalizedToken>
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Castillo Tovar herbarium] - holotype, MSU and FLAS - isotypes), GB KY271791.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="2" pageNumber="63" type="diagnosis">
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<paragraph pageId="2" pageNumber="63">Diagnosis.</paragraph>
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<paragraph pageId="2" pageNumber="63">
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<taxonomicName class="Pezizomycetes" family="Tuberaceae" genus="Tuber" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tuber aztecorum" order="Pezizales" pageId="2" pageNumber="63" phylum="Ascomycota" rank="species" species="aztecorum">Tuber aztecorum</taxonomicName>
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is a sister species to
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<taxonomicName lsidName="T. castilloi" pageId="2" pageNumber="63" rank="species" species="castilloi">T. castilloi</taxonomicName>
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, but
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<taxonomicName lsidName="T. castilloi" pageId="2" pageNumber="63" rank="species" species="castilloi">T. castilloi</taxonomicName>
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differs by having larger spores, 27-63
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<normalizedToken originalValue="×">x</normalizedToken>
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20-40
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, is without an irregular thickness to the cell wall on peridial hyphae and is associated mainly with
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<taxonomicName class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="2" pageNumber="63" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
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spp. Also resembles
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<taxonomicName lsidName="T. guevarai" pageId="2" pageNumber="63" rank="species" species="guevarai">T. guevarai</taxonomicName>
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but
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<taxonomicName lsidName="T. guevarai" pageId="2" pageNumber="63" rank="species" species="guevarai">T. guevarai</taxonomicName>
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has narrow spores that are 18-55
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<normalizedToken originalValue="×">x</normalizedToken>
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16-42
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<normalizedToken originalValue="µm">µm</normalizedToken>
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and cream-yellow fruiting bodies and rDNA variation.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="2" pageNumber="63" type="etymology">
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<paragraph pageId="2" pageNumber="63">Etymology.</paragraph>
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<paragraph pageId="2" pageNumber="63">
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<normalizedToken originalValue="“aztecorum”">"aztecorum"</normalizedToken>
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in reference to the ancient Aztec civilisation of Mexico.
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</paragraph>
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</subSubSection>
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<subSubSection lastPageId="4" lastPageNumber="65" pageId="2" pageNumber="63" type="description">
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<paragraph pageId="2" pageNumber="63">Description.</paragraph>
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<paragraph pageId="2" pageNumber="63">
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Ascomata 5-23
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<normalizedToken originalValue="×">x</normalizedToken>
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4-16
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<normalizedToken originalValue="×">x</normalizedToken>
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3-11 mm, subglobose, irregular, lobate or globose, light to orange brown or reddish-brown changing to dark brown when handled, finely verrucose or granulose, with 5-8 verrucae in 1 mm, solid, brittle, surface dry, base sessile. Peridium in cross-section undetachable <.5 mm wide, with one or several basal white to cream furrows or depressions that merge into veins. 5% KOH negative. Gleba marbled, white to greyish, white veins, some veins ending in the peridium. Odour fungoid to raw potato-like, taste not recorded.
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</paragraph>
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<paragraph lastPageId="3" lastPageNumber="64" pageId="2" pageNumber="63">
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Peridium 110-350
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<normalizedToken originalValue="µm">µm</normalizedToken>
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thick. Outer layer (epicutis) a pseudoparenchyma 62-250
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<normalizedToken originalValue="µm">µm</normalizedToken>
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thick, of hyphae 5-30
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<normalizedToken originalValue="µm">µm</normalizedToken>
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diam., versiform, angular or isodiametric, in some areas hyphae arranged perpendicular to the epicutis, hyaline to reddish-brown in mass in KOH, thick-walled (2
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<normalizedToken originalValue="µm">µm</normalizedToken>
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), without intracellular content. Surface hairs versiform, single hair-like hyphae or cystidia 53-97
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<normalizedToken originalValue="µm">µm</normalizedToken>
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long
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<normalizedToken originalValue="×">x</normalizedToken>
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4-5
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<normalizedToken originalValue="µm">µm</normalizedToken>
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at the base, tapered to the tip, some with septa, scattered or in clusters, brittle, thin-walled, hyaline in KOH. Other hyphae present are claviform, erect, cylindrical or sinuate with an irregular thickness to the cell wall that resembles knobs or
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<normalizedToken originalValue="“spines”">"spines"</normalizedToken>
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. Some globose or constricted hyphae emerging from isodiametric hyphae, 3-10
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<normalizedToken originalValue="µm">µm</normalizedToken>
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wide. Inner layer (subcutis) 50-225
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<normalizedToken originalValue="µm">µm</normalizedToken>
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thick, of prostrate and interwoven, hyphae gradually intermixing into gleba, hyaline in KOH and trypan blue, hyphae
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<pageBreakToken pageId="3" pageNumber="64" start="start">2</pageBreakToken>
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-5
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<normalizedToken originalValue="µm">µm</normalizedToken>
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wide. Some young specimens show noticeable prostrate cylindrical, claviform or vermiform hyphae along the subcutis that are thick-walled. Veins formed by hyaline, thin-walled, interwoven hyphae.
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</paragraph>
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<paragraph lastPageId="4" lastPageNumber="65" pageId="3" pageNumber="64">
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Ascospores subglobose, globose to broadly ellipsoid, 23-58
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<normalizedToken originalValue="×">x</normalizedToken>
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18-48
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<normalizedToken originalValue="µm">µm</normalizedToken>
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without ornamentation, alveoli 2-7
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<normalizedToken originalValue="µm">µm</normalizedToken>
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tall, 7-10 alveolar meshes along the spore length, 5-6 across, polygonal (4-6 sides), cell wall 2-3
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<normalizedToken originalValue="µm">µm</normalizedToken>
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thick. 1-spored asci have spores that are 42-58
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<normalizedToken originalValue="×">x</normalizedToken>
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27-48
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, 2-spored asci have spores that are 25-52
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<normalizedToken originalValue="×">x</normalizedToken>
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23-40
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, 3-spored asci have spores that are 27-40
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<normalizedToken originalValue="×">x</normalizedToken>
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20-30
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, 4-spored asci have spores that
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<pageBreakToken pageId="4" pageNumber="65" start="start">are</pageBreakToken>
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23-38
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<normalizedToken originalValue="×">x</normalizedToken>
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18-28
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, 5-spored asci have spores that are 25-32
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<normalizedToken originalValue="×">x</normalizedToken>
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18-25
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, yellowish to light brown in KOH and
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<normalizedToken originalValue="Melzer´s">Melzer's</normalizedToken>
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reagent. Asci globose, subglobose to broadly ellipsoid, without pedicel, 62-95
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<normalizedToken originalValue="×">x</normalizedToken>
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57-77 mm, hyaline in KOH, yellowish to brownish in
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<normalizedToken originalValue="Melzer´s">Melzer's</normalizedToken>
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reagent, thin-walled (immature asci thick-walled, up to 7.5
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<normalizedToken originalValue="µm">µm</normalizedToken>
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thick).
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</paragraph>
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<caption pageId="4" pageNumber="65">
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<paragraph pageId="4" pageNumber="65">
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Figure 1. Phylogenetic tree inferred under the maximum-likelihood (ML) criterion from the ITS rDNA alignment corresponding to the
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<taxonomicName class="Pezizomycetes" family="Tuberaceae" genus="Tuber" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tuber" order="Pezizales" pageId="4" pageNumber="65" phylum="Ascomycota" rank="genus">Tuber</taxonomicName>
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dataset. The tree was rooted using midpoint rooting. Numbers on the branches represent support values from 1,000 ML bootstrap replicates. The branches are scaled in terms of the expected number of substitutions per site. The phylogeny is rooted with species belonging to the Latisporum clade. Accession numbers in the sequence labels indicate sequences from Genbank.
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</paragraph>
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</caption>
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<caption pageId="4" pageNumber="65">
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<paragraph pageId="4" pageNumber="65">
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Figure 2.
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<normalizedToken originalValue="a–i">a-i</normalizedToken>
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<taxonomicName class="Pezizomycetes" family="Tuberaceae" genus="Tuber" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tuber aztecorum" order="Pezizales" pageId="4" pageNumber="65" phylum="Ascomycota" rank="species" species="aztecorum">Tuber aztecorum</taxonomicName>
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(holotype ITCV 993). a; Two ascomata showing the peridial surface (bar = 1 cm) b Ascoma in cross-section showing peridial surface and glebal surface (bar = 1 cm) c Peridial surface magnified showing the verrucose surface (bar = 1 mm) d Clusters of erect hyphae emanating from the peridial surface (bar =10
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<normalizedToken originalValue="µm">µm</normalizedToken>
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) e A single surface hair-like hypha (bar = 10
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<normalizedToken originalValue="µm">µm</normalizedToken>
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) f Cystidium (bar = 10
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<normalizedToken originalValue="µm">µm</normalizedToken>
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) g Cross section of peridium showing pseudoparenchyma-like epicutis (bar = 20
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<normalizedToken originalValue="µm">µm</normalizedToken>
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) h Ascospores within asci in surface view showing the alveoli (bar = 20
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<normalizedToken originalValue="µm">µm</normalizedToken>
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) i Ascospore within asci in surface view showing the alveoli magnified (bar = 20
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<normalizedToken originalValue="µm">µm</normalizedToken>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="4" pageNumber="65" type="distribution">
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<paragraph pageId="4" pageNumber="65">Distribution and Ecology.</paragraph>
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<paragraph pageId="4" pageNumber="65">
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MEXICO, state of Mexico La Puerta, National Park Nevado de Toluca. Hypogeous, gregarious in volcanic rock soil in an
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<taxonomicName class="Pinopsida" family="Pinaceae" genus="Abies" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Abies religiosa" order="Pinales" pageId="4" pageNumber="65" phylum="Tracheophyta" rank="species" species="religiosa">Abies religiosa</taxonomicName>
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forest at 3065 m. N 19°11.662', W099°48.537'. 29 July 2010.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="4" pageNumber="65" type="additional collections examined">
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<paragraph pageId="4" pageNumber="65">Additional collections examined.</paragraph>
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<paragraph pageId="4" pageNumber="65">Mexico, state of Mexico, La Puerta, National Park Nevado de Toluca, Guevara 1109 (paratype ITCV1109, GB KY271790), Guevara 1110 (paratype ITCV 1110), 29 July 2010.</paragraph>
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</subSubSection>
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</treatment>
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</document> |