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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.77.60862" ID-GBIF-Dataset="ebda1d51-fc41-45d4-b3b6-b8fa1813f4f4" ID-PMC="PMC7803722" ID-Pensoft-Pub="1314-4049-77-1" ID-Pensoft-UUID="F53D2641398253F988840BC0854594BB" ID-PubMed="33510579" ModsDocID="1314-4049-77-1" checkinTime="1609896229490" checkinUser="pensoft" docAuthor="Iturrieta-Gonzalez, Isabel, Garcia, Dania &amp; Gene, Josepa" docDate="2021" docId="58A7717F372851CDA78233F732E12516" docLanguage="en" docName="MycoKeys 77: 1-25" docOrigin="MycoKeys 77" docPubDate="2021-01-05" docSource="http://dx.doi.org/10.3897/mycokeys.77.60862" docTitle="Cladosporium pseudotenellum Iturrieta-Gonzalez, Dania Garcia, Gene 2021, sp. nov." docType="treatment" docVersion="4" id="F53D2641398253F988840BC0854594BB" lastPageNumber="1" masterDocId="F53D2641398253F988840BC0854594BB" masterDocTitle="Novel species of Cladosporium from environmental sources in Spain" masterLastPageNumber="25" masterPageNumber="1" pageNumber="1" updateTime="1668136601215" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Novel species of Cladosporium from environmental sources in Spain</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Iturrieta-Gonzalez, Isabel</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-8426-9530</mods:nameIdentifier>
<mods:affiliation>Unitat de Micologia, Facultat de Medicina i Ciencies de la Salut and IISPV, Universitat Rovira i Virgili, 43201, Reus, Tarragona, Spain</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Garcia, Dania</mods:namePart>
<mods:affiliation>Unitat de Micologia, Facultat de Medicina i Ciencies de la Salut and IISPV, Universitat Rovira i Virgili, 43201, Reus, Tarragona, Spain</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Gene, Josepa</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-6195-9299</mods:nameIdentifier>
<mods:affiliation>Unitat de Micologia, Facultat de Medicina i Ciencies de la Salut and IISPV, Universitat Rovira i Virgili, 43201, Reus, Tarragona, Spain</mods:affiliation>
<mods:nameIdentifier type="email">josepa.gene@urv.cat</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2021</mods:date>
<mods:detail type="pubDate">
<mods:number>2021-01-05</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>77</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>1</mods:start>
<mods:end>25</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.77.60862</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.77.60862</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-77-1</mods:identifier>
<mods:identifier type="Pensoft-UUID">F53D2641398253F988840BC0854594BB</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="176435572" LSID="urn:lsid:plazi:treatment:58A7717F372851CDA78233F732E12516" httpUri="http://treatment.plazi.org/id/58A7717F372851CDA78233F732E12516" lastPageNumber="1" pageId="0" pageNumber="1">
<subSubSection pageId="0" pageNumber="1" type="nomenclature">
<paragraph pageId="0" pageNumber="1">
<taxonomicName LSID="58A7717F-3728-51CD-A782-33F732E12516" authority="Iturrieta-González, Dania García, Gené" authorityName="Iturrieta-Gonzalez, Dania Garcia, Gene" authorityYear="2021" class="Dothideomycetes" family="Cladosporiaceae" genus="Cladosporium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Cladosporium pseudotenellum" order="Capnodiales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="pseudotenellum" status="sp. nov.">
Cladosporium pseudotenellum
<normalizedToken originalValue="Iturrieta-González">Iturrieta-Gonzalez</normalizedToken>
, Dania
<normalizedToken originalValue="García">Garcia</normalizedToken>
,
<normalizedToken originalValue="Gené">Gene</normalizedToken>
</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 7" captionStartId="F8" captionText="Figure 7. Cladosporium pseudotenellum (ex-type FMR 16231) a-c colonies on PDA, OA and SNA after 14 days at 25 ° C d-e conidiophores f conidia. Scale bars: 10 mm (a-c); 10 μm (d-f)." figureDoi="10.3897/mycokeys.77.60862.figure7" httpUri="https://binary.pensoft.net/fig/494242" pageId="0" pageNumber="1">Fig. 7</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="etymology">
<paragraph pageId="0" pageNumber="1">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="1">
The name refers to &quot;
<taxonomicName lsidName="C. tenellum" pageId="0" pageNumber="1" rank="species" species="tenellum">
<emphasis italics="true" pageId="0" pageNumber="1">C. tenellum</emphasis>
</taxonomicName>
&quot;, the closest phylogenetic species.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="materials_examined">
<paragraph pageId="0" pageNumber="1">Type.</paragraph>
<paragraph pageId="0" pageNumber="1">
Spain, Catalonia, Tarragona province, Reus, garden soil, Feb. 2017,
<emphasis italics="true" pageId="0" pageNumber="1">
I.
<normalizedToken originalValue="Iturrieta-González">Iturrieta-Gonzalez</normalizedToken>
</emphasis>
(holotype CBS H-24473; cultures ex-type FMR 16231, CBS 146922).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="description">
<paragraph pageId="0" pageNumber="1">Description.</paragraph>
<paragraph pageId="0" pageNumber="1">
<emphasis italics="true" pageId="0" pageNumber="1">Mycelium</emphasis>
in vitro superficial and immersed, composed of septate, branched, subhyaline to pale brown, smooth-walled, occasionally tuberculate and with abundant swellings, hyphae, 2-3(-4.5)
<normalizedToken originalValue="μm">μm</normalizedToken>
wide.
<emphasis italics="true" pageId="0" pageNumber="1">Conidiophores</emphasis>
macronematous, arising laterally or terminally from hyphae, erect to slightly flexuous, non-nodulose, occasionally geniculate at the apex, septate, unbranched, occasionally branched, up to 146
<normalizedToken originalValue="μm">μm</normalizedToken>
long, 2.5-3
<normalizedToken originalValue="μm">μm</normalizedToken>
wide, pale brown, smooth to slightly verruculose.
<emphasis italics="true" pageId="0" pageNumber="1">Conidiogenous cells</emphasis>
integrated, terminal or intercalary, cylindrical, sometimes geniculate, 15-32
<normalizedToken originalValue="×">x</normalizedToken>
2.5-3
<normalizedToken originalValue="μm">μm</normalizedToken>
, with up to five conidiogenous loci, thickened, darkened and refractive, often crowded at the apex.
<emphasis italics="true" pageId="0" pageNumber="1">Ramoconidia</emphasis>
rarely formed, 0(-1)-septate, ellipsoidal to subcylindrical, 9-14.5
<normalizedToken originalValue="×">x</normalizedToken>
4-5.5
<normalizedToken originalValue="μm">μm</normalizedToken>
[av. (
<normalizedToken originalValue="±">+/-</normalizedToken>
SD) 11.6 (
<normalizedToken originalValue="±">+/-</normalizedToken>
1.60)
<normalizedToken originalValue="×">x</normalizedToken>
4.6 (
<normalizedToken originalValue="±">+/-</normalizedToken>
0.44)], pale brown, verruculose.
<emphasis italics="true" pageId="0" pageNumber="1">Conidia</emphasis>
forming branched chains, with up to four conidia in the terminal unbranched part, aseptate, pale brown, verruculose to verrucose;
<emphasis italics="true" pageId="0" pageNumber="1">small terminal conidia</emphasis>
subglobose to obovoid, 4-7
<normalizedToken originalValue="×">x</normalizedToken>
3-5
<normalizedToken originalValue="μm">μm</normalizedToken>
[av. (
<normalizedToken originalValue="±">+/-</normalizedToken>
SD) 5.8 (
<normalizedToken originalValue="±">+/-</normalizedToken>
0.77)
<normalizedToken originalValue="×">x</normalizedToken>
3.9 (
<normalizedToken originalValue="±">+/-</normalizedToken>
0.60)];
<emphasis italics="true" pageId="0" pageNumber="1">intercalary conidia</emphasis>
ellipsoidal to limoniform, 6-8.5
<normalizedToken originalValue="×">x</normalizedToken>
3-5
<normalizedToken originalValue="μm">μm</normalizedToken>
[av. (
<normalizedToken originalValue="±">+/-</normalizedToken>
SD) 7.4 (
<normalizedToken originalValue="±">+/-</normalizedToken>
0.73)
<normalizedToken originalValue="×">x</normalizedToken>
3.8 (
<normalizedToken originalValue="±">+/-</normalizedToken>
0.50)];
<emphasis italics="true" pageId="0" pageNumber="1">secondary ramoconidia</emphasis>
0(-2)-septate, ellipsoidal to subcylindrical, 7-12.5
<normalizedToken originalValue="×">x</normalizedToken>
4-5
<normalizedToken originalValue="μm">μm</normalizedToken>
[av. (
<normalizedToken originalValue="±">+/-</normalizedToken>
SD) 9.6 (
<normalizedToken originalValue="±">+/-</normalizedToken>
1.76)
<normalizedToken originalValue="×">x</normalizedToken>
4.4 (
<normalizedToken originalValue="±">+/-</normalizedToken>
0.33)] with 1-3 distal hila.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="description">
<paragraph pageId="0" pageNumber="1">Culture characteristics</paragraph>
<paragraph pageId="0" pageNumber="1">(14 d at 25 °C). Colonies on OA reaching 21-22 mm diam., olive (2F8/2F4), flat, velvety, aerial mycelium scarce, margin fimbriate; reverse dark green (30F8) to black. On PDA attaining 29-30 mm diam., olive gray (3E2/3F2), paler at the periphery, radially folded, velvety, aerial mycelium scarce, margin slightly lobate; reverse dark green (30F8) to black. On SNA reaching 21-22 mm diam., olive (2F8), flat, slightly powdery, aerial mycelium scarce, margin fimbriate; reverse dark green (30F8) to black.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="cardinal temperature for growth">
<paragraph pageId="0" pageNumber="1">Cardinal temperature for growth.</paragraph>
<paragraph pageId="0" pageNumber="1">Optimum 20 °C, maximum 30 °C, minimum 5 °C.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="distribution">
<paragraph pageId="0" pageNumber="1">Distribution.</paragraph>
<paragraph pageId="0" pageNumber="1">Spain.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="1" type="notes">
<paragraph pageId="0" pageNumber="1">Notes.</paragraph>
<paragraph pageId="0" pageNumber="1">
Based on the phylogeny of the
<taxonomicName lsidName="C. herbarum" pageId="0" pageNumber="1" rank="species" species="herbarum">
<emphasis italics="true" pageId="0" pageNumber="1">C. herbarum</emphasis>
</taxonomicName>
complex (Fig.
<figureCitation captionStart="Figure 2" captionStartId="F3" captionText="Figure 2. Maximum likelihood (ML) tree obtained from the combined analysis of ITS, tef 1 and act sequences of 58 strains from C. herbarum complex. The tree is rooted with Cercospora beticola CBS 116456. Numbers on the branches represent ML bootstrap support values (MLBS) ≥ 70 %, followed by Maximum Parsimony bootstrap support values (PBS) ≥ 70 % and Bayesian posterior probabilities (PP) ≥ 0.95, lower values are indicate as &quot; - &quot;. Bold branches indicate MLBS / PBS / PP of 100 / 100 / 1. Names of species newly described are indicated in bold. Branch lengths are proportional to distance. T Ex-type strain. ET ex-epitype strain. NT ex-neotype strain." figureDoi="10.3897/mycokeys.77.60862.figure2" httpUri="https://binary.pensoft.net/fig/494237" pageId="0" pageNumber="1">2</figureCitation>
),
<taxonomicName lsidName="C. pseudotenellum" pageId="0" pageNumber="1" rank="species" species="pseudotenellum">
<emphasis italics="true" pageId="0" pageNumber="1">C. pseudotenellum</emphasis>
</taxonomicName>
is closely related with
<taxonomicName lsidName="C. tenellum" pageId="0" pageNumber="1" rank="species" species="tenellum">
<emphasis italics="true" pageId="0" pageNumber="1">C. tenellum</emphasis>
</taxonomicName>
, a species originally described from hypersaline water in Israel, later found on
<taxonomicName class="Ascomycetes" family="Erysiphaceae" genus="Phyllactinia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Phyllactinia" order="Erysiphales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="1">Phyllactinia</emphasis>
</taxonomicName>
sp. (
<taxonomicName class="Ascomycetes" family="Erysiphaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="" order="Erysiphales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="family">Erysiphaceae</taxonomicName>
), and in indoor air samples collected in the USA (
<bibRefCitation DOI="https://doi.org/10.3114/sim.2007.58.05" author="Schubert, K" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="1" pagination="105 - 156" refId="B42" refString="Schubert, K, Groenewald, JZ, Braun, U, Dijksterhuis, J, Starink, M, Dijksterhuis, J, Starink, M, Hill, CF, Zalar, P, de Hoog, GS, Crous, PW, 2007. Biodiversity in the Cladosporium herbarum complex (Davidiellaceae, Capnodiales), with standardisation of methods for Cladosporium taxonomy and diagnostics. Studies in Mycology 58: 105 - 156, DOI: https://doi.org/10.3114/sim.2007.58.05" title="Biodiversity in the Cladosporium herbarum complex (Davidiellaceae, Capnodiales), with standardisation of methods for Cladosporium taxonomy and diagnostics." url="https://doi.org/10.3114/sim.2007.58.05" volume="58" year="2007">Schubert et al. 2007</bibRefCitation>
;
<bibRefCitation DOI="https://doi.org/10.3114/sim0003" author="Bensch, K" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="1" pagination="1 - 401" refId="B5" refString="Bensch, K, Braun, U, Groenewald, JZ, Crous, PW, 2012. The genus Cladosporium. Studies in Mycology 72: 1 - 401, DOI: https://doi.org/10.3114/sim0003" title="The genus Cladosporium." url="https://doi.org/10.3114/sim0003" volume="72" year="2012">Bensch et al. 2012</bibRefCitation>
,
<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2018.03.002" author="Bensch, K" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="1" pagination="177 - 301" refId="B7" refString="Bensch, K, Groenewald, JZ, Meijer, M, Dijksterhuis, J, Jurjevic, Z, Dijksterhuis, J, Jurjevic, Z, Andersen, B, Houbraken, J, Crous, PW, Samson, RA, 2018. Cladosporium species in indoor environments. Studies in Mycology 89: 177 - 301, DOI: https://doi.org/10.1016/j.simyco.2018.03.002" title="Cladosporium species in indoor environments." url="https://doi.org/10.1016/j.simyco.2018.03.002" volume="89" year="2018">2018</bibRefCitation>
). Our species differs from
<taxonomicName lsidName="C. tenellum" pageId="0" pageNumber="1" rank="species" species="tenellum">
<emphasis italics="true" pageId="0" pageNumber="1">C. tenellum</emphasis>
</taxonomicName>
in the absence of micronematous conidiophores and in having shorter macronematous conidiophores (up to 146
<normalizedToken originalValue="μm">μm</normalizedToken>
vs up to 200
<normalizedToken originalValue="μm">μm</normalizedToken>
), shorter conidiogenous cells (15-32
<normalizedToken originalValue="μm">μm</normalizedToken>
vs 6-40
<normalizedToken originalValue="μm">μm</normalizedToken>
), with few conidiogenous loci (up to five vs up to 10 or more in
<taxonomicName lsidName="C. tenellum" pageId="0" pageNumber="1" rank="species" species="tenellum">
<emphasis italics="true" pageId="0" pageNumber="1">C. tenellum</emphasis>
</taxonomicName>
), and shorter ramoconidia (9-14.5 vs up to 32
<normalizedToken originalValue="μm">μm</normalizedToken>
). In addition, terminal and intercalary conidia in
<taxonomicName lsidName="C. pseudotenellum" pageId="0" pageNumber="1" rank="species" species="pseudotenellum">
<emphasis italics="true" pageId="0" pageNumber="1">C. pseudotenellum</emphasis>
</taxonomicName>
are aseptate, while those of
<taxonomicName lsidName="C. tenellum" pageId="0" pageNumber="1" rank="species" species="tenellum">
<emphasis italics="true" pageId="0" pageNumber="1">C. tenellum</emphasis>
</taxonomicName>
are 0-1(-3)-septate (
<bibRefCitation DOI="https://doi.org/10.3114/sim.2007.58.05" author="Schubert, K" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="1" pagination="105 - 156" refId="B42" refString="Schubert, K, Groenewald, JZ, Braun, U, Dijksterhuis, J, Starink, M, Dijksterhuis, J, Starink, M, Hill, CF, Zalar, P, de Hoog, GS, Crous, PW, 2007. Biodiversity in the Cladosporium herbarum complex (Davidiellaceae, Capnodiales), with standardisation of methods for Cladosporium taxonomy and diagnostics. Studies in Mycology 58: 105 - 156, DOI: https://doi.org/10.3114/sim.2007.58.05" title="Biodiversity in the Cladosporium herbarum complex (Davidiellaceae, Capnodiales), with standardisation of methods for Cladosporium taxonomy and diagnostics." url="https://doi.org/10.3114/sim.2007.58.05" volume="58" year="2007">Schubert et al. 2007</bibRefCitation>
;
<bibRefCitation DOI="https://doi.org/10.3114/sim0003" author="Bensch, K" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="1" pagination="1 - 401" refId="B5" refString="Bensch, K, Braun, U, Groenewald, JZ, Crous, PW, 2012. The genus Cladosporium. Studies in Mycology 72: 1 - 401, DOI: https://doi.org/10.3114/sim0003" title="The genus Cladosporium." url="https://doi.org/10.3114/sim0003" volume="72" year="2012">Bensch et al. 2012</bibRefCitation>
).
</paragraph>
<caption doi="10.3897/mycokeys.77.60862.figure7" httpUri="https://binary.pensoft.net/fig/494242" pageId="0" pageNumber="1" start="Figure 7" startId="F8">
<paragraph pageId="0" pageNumber="1">
<emphasis bold="true" pageId="0" pageNumber="1">Figure 7.</emphasis>
<taxonomicName authorityName="Iturrieta-Gonzalez, Dania Garcia, Gene" authorityYear="2021" class="Dothideomycetes" family="Cladosporiaceae" genus="Cladosporium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Cladosporium pseudotenellum" order="Capnodiales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="pseudotenellum">
<emphasis italics="true" pageId="0" pageNumber="1">Cladosporium pseudotenellum</emphasis>
</taxonomicName>
(ex-type FMR 16231)
<emphasis bold="true" pageId="0" pageNumber="1">a-c</emphasis>
colonies on PDA, OA and SNA after 14 days at 25 °C
<emphasis bold="true" pageId="0" pageNumber="1">d-e</emphasis>
conidiophores
<emphasis bold="true" pageId="0" pageNumber="1">f</emphasis>
conidia. Scale bars: 10 mm (
<emphasis bold="true" pageId="0" pageNumber="1">a-c</emphasis>
); 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="1">d-f</emphasis>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>