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<document ID-CLB-Dataset="293876" ID-DOI="http://dx.doi.org/10.3897/mycokeys.104.112149" ID-GBIF-Dataset="e69a6c20-6825-4c4f-8b82-24bf02929ae5" ID-PMC="PMC11040200" ID-Pensoft-Pub="1314-4049-104-9" ID-Pensoft-UUID="BA6C35A352D8524293F9CFED6D649F88" ID-PubMed="38665970" ModsDocID="1314-4049-104-9" checkinTime="1713281631710" checkinUser="pensoft" docAuthor="Phookamsak, Rungtiwa, Hongsanan, Sinang, Bhat, Darbhe Jayarama, Wanasinghe, Dhanushka N., Promputtha, Itthayakorn, Suwannarach, Nakarin, Kumla, Jaturong, Xie, Ning, Dawoud, Turki M., Mortimer, Peter E., Xu, Jianchu &amp; Lumyong, Saisamorn" docDate="2024" docId="49C2007018C2565087C4419C204D1167" docLanguage="en" docName="MycoKeys 104: 9-50" docOrigin="MycoKeys 104" docPubDate="2024-04-16" docSource="http://dx.doi.org/10.3897/mycokeys.104.112149" docTitle="Trichobotrys sinensis Phookamsak, Bhat &amp; Hongsanan 2024, sp. nov." docType="treatment" docVersion="4" id="BA6C35A352D8524293F9CFED6D649F88" lastPageNumber="9" masterDocId="BA6C35A352D8524293F9CFED6D649F88" masterDocTitle="Exploring ascomycete diversity in Yunnan II: Introducing three novel species in the suborder Massarineae (Dothideomycetes, Pleosporales) from fern and grasses" masterLastPageNumber="50" masterPageNumber="9" pageNumber="9" updateTime="1732673929831" updateUser="ExternalLinkService">
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<mods:title id="16176BBD0DB4370F588055123E702A82">Exploring ascomycete diversity in Yunnan II: Introducing three novel species in the suborder Massarineae (Dothideomycetes, Pleosporales) from fern and grasses</mods:title>
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<mods:namePart id="AEE66758EF450A704CC5182EEA42B571">Phookamsak, Rungtiwa</mods:namePart>
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<mods:namePart id="C78071FF56DAA90E4142ACED09C8AFF1">Hongsanan, Sinang</mods:namePart>
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<mods:affiliation id="24A5D73F3C72C11A953CC67C42ECFA6B">Center of Excellence in Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand &amp; Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China</mods:affiliation>
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<mods:affiliation id="96B97ED2A31786A7B09DDFA69820C3BB">Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia &amp; Vishnugupta Vishwavidyapeetam, Ashoke, Gokarna 581326, India</mods:affiliation>
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<mods:affiliation id="2C1E151C6627A55AE6CD69C3EC92AD63">Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan Province, China &amp; Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe, 654400, Yunnan Province, China &amp; CIFOR-ICRAF China Program, World Agroforestry (ICRAF), Kunming 650201, Yunnan Province, China &amp; Center for Mountain Futures (CMF), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan Province, China</mods:affiliation>
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<mods:namePart id="9E8CDBCFF77B0B666503B941E9811925">Xie, Ning</mods:namePart>
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<mods:affiliation id="59D2F53D2BDF041A133893031C9E73C3">Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China</mods:affiliation>
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<mods:affiliation id="704C29862806C5B58C6A471E7CCDC11C">Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia</mods:affiliation>
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<mods:namePart id="EC8A5C233C81F60E85B48901D179DD75">Mortimer, Peter E.</mods:namePart>
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<mods:affiliation id="D670619F439747FF815AA9B6ADDB052C">Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan Province, China &amp; Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe, 654400, Yunnan Province, China</mods:affiliation>
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<mods:namePart id="C523F33235BA2C0C70ECCF60F7DDBFB3">Xu, Jianchu</mods:namePart>
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<mods:affiliation id="AC0F89524F3D4A7B395368A659B30EE9">Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan Province, China &amp; Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe, 654400, Yunnan Province, China &amp; CIFOR-ICRAF China Program, World Agroforestry (ICRAF), Kunming 650201, Yunnan Province, China &amp; Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan Province, China</mods:affiliation>
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<mods:namePart id="BCF72E7D37772623867E2CFBE2F903D4">Lumyong, Saisamorn</mods:namePart>
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<mods:affiliation id="279DEE3AE980960B796EAF975497C6DF">Center of Excellence in Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand &amp; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand</mods:affiliation>
<mods:nameIdentifier id="30FABE552162D742EF07C898D5F01598" type="email">scboi009@gmail.com</mods:nameIdentifier>
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<mods:typeOfResource id="13268E69479089A903EC54877052114A">text</mods:typeOfResource>
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<mods:date id="4B0BD1200AF1F42D5A685E182D47C5BA">2024</mods:date>
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<mods:number id="26E3EF393EB20CE378EA59CE21287BA2">2024-04-16</mods:number>
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<mods:number id="86BA2FA77E2AD46139EC450B7D5F7FDB">104</mods:number>
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<mods:start id="814958509D43B6DD302EA5CDCED318D8">9</mods:start>
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<treatment id="49C2007018C2565087C4419C204D1167" ID-GBIF-Taxon="225691034" LSID="urn:lsid:plazi:treatment:49C2007018C2565087C4419C204D1167" httpUri="http://treatment.plazi.org/id/49C2007018C2565087C4419C204D1167" lastPageNumber="9" pageId="0" pageNumber="9" scope_class="Dothideomycetes" scope_family="Dictyosporiaceae" scope_order="Pleosporales">
<subSubSection id="57FB961A208D5457CF0472F48A0B14CF" pageId="0" pageNumber="9" type="nomenclature">
<paragraph id="2CD9DDD086030ABDD65AB876C91729DD" pageId="0" pageNumber="9">
<taxonomicName id="21DA5F7F12187006232CB6CD317ECB56" LSID="49C20070-18C2-5650-87C4-419C204D1167" authority="Phookamsak, Bhat &amp; Hongsanan" authorityName="Phookamsak, Bhat &amp; Hongsanan" authorityYear="2024" class="Dothideomycetes" family="Dictyosporiaceae" genus="Trichobotrys" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobotrys sinensis" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="species" species="sinensis" status="sp. nov.">Trichobotrys sinensis Phookamsak, Bhat &amp; Hongsanan</taxonomicName>
<taxonomicNameLabel id="EF08E24A8B705434DEC93AF83CFA3D54" pageId="0" pageNumber="9">sp. nov.</taxonomicNameLabel>
</paragraph>
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<subSubSection id="E05FE0FC061BFCDE661F622137698684" pageId="0" pageNumber="9" type="description">
<paragraph id="ADEB152E6D58DA811B91B312830203FA" pageId="0" pageNumber="9">
<figureCitation id="4E2F2B2F865B2D2E3AB618BD8DF6B5C0" captionStart="Figure 5" captionStartId="F5" captionText="Figure 5. Trichobotrys sinensis (KUN-HKAS 129041, holotype) A, B the appearance of colonies on the host surface C mycelium D-H conidiophores bearing conidiogenous cells and conidia I conidia in a short acropetal chain J-N conidia O culture characteristics on PDA P conidioma forming on PDA after eight weeks Q pycnidial wall R-T conidiogenous cells (note: T = stained in Congo red) U conidia. Scale bars: 100 μm (P); 50 μm (C); 10 μm (D-H, Q-U); 5 μm (J-N)." figureDoi="10.3897/mycokeys.104.112149.figure5" httpUri="https://binary.pensoft.net/fig/1026396" pageId="0" pageNumber="9">Fig. 5</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="5D4B00BA48B21973B43C25552978FB9F" pageId="0" pageNumber="9" type="etymology">
<paragraph id="C824F12C125266F4367A9714A215BC82" pageId="0" pageNumber="9">Etymology.</paragraph>
<paragraph id="E26D7061E90EA87A64E0EA7AA5E8CEC7" pageId="0" pageNumber="9">
The specific epithet &quot;
<emphasis id="2EBFE35379ECE9A8F2B6D2F1B4418F95" italics="true" pageId="0" pageNumber="9">sinensis</emphasis>
&quot; refers to the country, China, where the holotype was collected.
</paragraph>
<caption id="7CDB1F721EF5A602F9FF31C58C2FD467" doi="10.3897/mycokeys.104.112149.figure5" httpUri="https://binary.pensoft.net/fig/1026396" pageId="0" pageNumber="9" start="Figure 5" startId="F5">
<paragraph id="04889A2231ED6105E14D42AD894F2BDF" pageId="0" pageNumber="9">
<emphasis id="5CEB6091C8F1757195D4346B0FA3D4F5" bold="true" pageId="0" pageNumber="9">Figure 5.</emphasis>
<taxonomicName id="3D6E376973AA5CA01D291B3806C9716B" authorityName="Phookamsak, Bhat &amp; Hongsanan" authorityYear="2024" class="Dothideomycetes" family="Dictyosporiaceae" genus="Trichobotrys" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobotrys sinensis" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="species" species="sinensis">
<emphasis id="FD54750155A3241F511327AE3BF6AFFD" italics="true" pageId="0" pageNumber="9">Trichobotrys sinensis</emphasis>
</taxonomicName>
(KUN-HKAS 129041, holotype)
<emphasis id="9DA073A8CC72298FFA3D261FFCED7063" bold="true" pageId="0" pageNumber="9">A, B</emphasis>
the appearance of colonies on the host surface
<emphasis id="132AD8C2480987EFA73A8D17376D9756" bold="true" pageId="0" pageNumber="9">C</emphasis>
mycelium
<emphasis id="ABC9E179B2815A9497B388BD2C8D6ECB" bold="true" pageId="0" pageNumber="9">D-H</emphasis>
conidiophores bearing conidiogenous cells and conidia
<emphasis id="836AA5B02D358DA809A4C91C1024A631" bold="true" pageId="0" pageNumber="9">I</emphasis>
conidia in a short acropetal chain
<emphasis id="81D49FA998C2BBF1F8BF3968601131AC" bold="true" pageId="0" pageNumber="9">J-N</emphasis>
conidia
<emphasis id="442DAA955EF21EDE890A735274AE9955" bold="true" pageId="0" pageNumber="9">O</emphasis>
culture characteristics on PDA
<emphasis id="5FBA35F6FAEA5AC88D1850715355D735" bold="true" pageId="0" pageNumber="9">P</emphasis>
conidioma forming on PDA after eight weeks
<emphasis id="A7ABA83B1AA200D25CC590DCEE107B21" bold="true" pageId="0" pageNumber="9">Q</emphasis>
pycnidial wall
<emphasis id="C7F70E42B71C95331DB5A532621DD4C8" bold="true" pageId="0" pageNumber="9">R-T</emphasis>
conidiogenous cells (note:
<emphasis id="F7B380AB0AFD45D7D2FA699EB4ECD59D" bold="true" pageId="0" pageNumber="9">T</emphasis>
= stained in Congo red)
<emphasis id="BD5D1B25E156429D059E06A605A83561" bold="true" pageId="0" pageNumber="9">U</emphasis>
conidia. Scale bars: 100
<normalizedToken id="C3F19DECB65106EFD63579F4814DF6E8" originalValue="μm">μm</normalizedToken>
(
<emphasis id="C000FD1C50DAD59A9C043BC927882576" bold="true" pageId="0" pageNumber="9">P</emphasis>
); 50
<normalizedToken id="31FC762810412D38E8B459D994F6495A" originalValue="μm">μm</normalizedToken>
(
<emphasis id="68358E7999CBEF36823F0F371F688855" bold="true" pageId="0" pageNumber="9">C</emphasis>
); 10
<normalizedToken id="3586DB9FD0763819CCCB7C18C054D71F" originalValue="μm">μm</normalizedToken>
(
<emphasis id="A45D2E578752D6FC19F13D2C0D7433BC" bold="true" pageId="0" pageNumber="9">D-H, Q-U</emphasis>
); 5
<normalizedToken id="BA396BF8CDC3315314768A9DB9FC0D72" originalValue="μm">μm</normalizedToken>
(
<emphasis id="7B9F84D8671B17790442C8C1A1DE3D69" bold="true" pageId="0" pageNumber="9">J-N</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="3B83EC8C64538884BCFDBF2B59E0B38B" pageId="0" pageNumber="9" type="holotype">
<paragraph id="F433CB08D57790C4A9EF95C8689B6B34" pageId="0" pageNumber="9">Holotype.</paragraph>
<paragraph id="A7C13BA0D68B5460B60F8A705D84C071" pageId="0" pageNumber="9">KUN-HKAS 129041.</paragraph>
</subSubSection>
<subSubSection id="08F022685BA08C0A4C0C14E350663CDB" pageId="0" pageNumber="9" type="description">
<paragraph id="E66998EBC1C42CFF8840C752993F1ABB" pageId="0" pageNumber="9">Description.</paragraph>
<paragraph id="6760A942C6CFDF8FC640F359E07D8422" pageId="0" pageNumber="9">
Saprobic on dead culm of
<taxonomicName id="14124C1EAE8184C16401E3F6160B2CCE" class="Monocotyledoneae" family="Poaceae" genus="Brachiaria" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Brachiaria mutica" order="Graminales" pageId="0" pageNumber="9" phylum="Angiospermae" rank="species" species="mutica">
<emphasis id="0EFB776CBA967F3F426007EAA24C3E88" italics="true" pageId="0" pageNumber="9">Brachiaria mutica</emphasis>
</taxonomicName>
, submerged in a small stream.
<emphasis id="103FE6A84598143FDD89FDF273F12CB5" bold="true" italics="true" pageId="0" pageNumber="9">Sexual morph</emphasis>
: Undetermined.
<emphasis id="243A35C75A0B0A7740C67F1A5DD465F2" bold="true" pageId="0" pageNumber="9">
<emphasis id="5ADDE98DD617C4EA83D05A3B73711B1D" italics="true" pageId="0" pageNumber="9">Asexual morph</emphasis>
:
<emphasis id="B56C907483CC2CA2AB826B832B8EC06B" italics="true" pageId="0" pageNumber="9">Colonies</emphasis>
</emphasis>
dull, black, effuse, visible as hairy fluffy on the host.
<emphasis id="0C5DED544A9F8B05B3370BBA342F9BE8" bold="true" italics="true" pageId="0" pageNumber="9">Mycelia</emphasis>
up to 1 mm long, 2-4
<normalizedToken id="1D86710D313E6F7E48A41FCFA3A5FF16" originalValue="µm">µm</normalizedToken>
wide, superficial, composed of brown to dark brown, branched, septate, thick-walled, echinulate hyphae.
<emphasis id="23DFE78B0628291D1A84DAFD78618868" bold="true" italics="true" pageId="0" pageNumber="9">Conidiophores</emphasis>
(9-)15-40(-70)
<normalizedToken id="D895071063D896266910778F9D2935FF" originalValue="×">x</normalizedToken>
2-4
<normalizedToken id="4AFE02FA1F60417F7A234B3A54AFB381" originalValue="µm">µm</normalizedToken>
(
<emphasis id="D60C90D5154DC8853E04DC81865C2FDF" italics="true" pageId="0" pageNumber="9"></emphasis>
= 26.9
<normalizedToken id="73DB4C62A7EC7DE6F5825464C4EDC8DF" originalValue="×">x</normalizedToken>
3.3
<normalizedToken id="C701D9402CA638AE1657E154B0C8135F" originalValue="μm">μm</normalizedToken>
, n = 30), sometimes reduced to conidiogenous cells, macronematous, mononematous, straight or flexuous, brown to dark brown, septate, verruculose or echinulate, bearing short, lateral, unciform, fertile branches, with setiform apex.
<emphasis id="3320002B5C3702C5855D484FC324BA44" bold="true" italics="true" pageId="0" pageNumber="9">Conidiogenous cells</emphasis>
1-3.5
<normalizedToken id="74741D2451864CE5666B7DA451DC702D" originalValue="×">x</normalizedToken>
2.5-5
<normalizedToken id="686688EBED74AC25A58DF06D1DA72DB8" originalValue="µm">µm</normalizedToken>
(
<emphasis id="8BBC8F33D784E6135B71D209F8F8D41E" italics="true" pageId="0" pageNumber="9"></emphasis>
= 2.1
<normalizedToken id="025DAB061ADB688D2102B8E57B5B1950" originalValue="×">x</normalizedToken>
2.5
<normalizedToken id="023E9BC7D2AA1271D4DEC165C2934934" originalValue="μm">μm</normalizedToken>
, n = 30), polyblastic, subhyaline to pale brown, ellipsoidal or hemispherical (2.5-5
<normalizedToken id="7267A45D6C43F1753D853833E17CAC56" originalValue="×">x</normalizedToken>
3.5-6
<normalizedToken id="66147A6486EC1E0893C75C8C97247A81" originalValue="µm">µm</normalizedToken>
), intercalary or terminal, integrated or discrete, sometimes denticulate on branches.
<emphasis id="1F3C5E507481D605EEFA6F788689152D" bold="true" italics="true" pageId="0" pageNumber="9">Conidia</emphasis>
7-11
<normalizedToken id="3BB3FD3D139965824A3895BA44FA98AA" originalValue="×">x</normalizedToken>
8-12
<normalizedToken id="B2EE1D8D7052305A06CB5947F8BAE694" originalValue="µm">µm</normalizedToken>
(
<emphasis id="FAAF9BA14717DCAA3DD5B7A4AFB51658" italics="true" pageId="0" pageNumber="9"></emphasis>
= 10
<normalizedToken id="8E7C7A8FC8735728FFDC3C9676563418" originalValue="×">x</normalizedToken>
10
<normalizedToken id="AA164C32377AF07B980C0D5CB7874DB4" originalValue="μm">μm</normalizedToken>
, n = 30) simple, solitary, brown to dark brown, spherical, aseptate, verruculose; sometimes in short acropetal chains.
<emphasis id="A01440DA3EEAF9CEBD201B3219DD886F" italics="true" pageId="0" pageNumber="9">In vitro</emphasis>
<emphasis id="EF9CFAFB93DBC2E49F4C310614240D25" bold="true" italics="true" pageId="0" pageNumber="9">Conidiomata</emphasis>
280-470
<normalizedToken id="651964117E4AA6B150B8F3E18D1E292A" originalValue="µm">µm</normalizedToken>
high, 280-570
<normalizedToken id="02A56ACB74F61251AEF070F5CC4ABC64" originalValue="µm">µm</normalizedToken>
diam., black, pycnidial, solitary or clustered in a small group (2-4-loculate), scattered to gregarious, globose to subglobose, glabrous, covered by brown to dark brown mycelium, becoming a packed pycnidial wall, ostiolate, with inconspicuous, minute papilla.
<emphasis id="E190A8FB77411962DD53DC5B110943AE" bold="true" italics="true" pageId="0" pageNumber="9">Pycnidial wall</emphasis>
20-35
<normalizedToken id="28520253C581EAA07B1B80F2B85771D7" originalValue="µm">µm</normalizedToken>
wide, thick-walled of unequal thickness, thicker at the base, composed of multi-layered, dark brown to black pseudoparenchymatous cells, outer layers composed of
<emphasis id="6919867458FE941EF8D6F598D5852A44" italics="true" pageId="0" pageNumber="9">textura intricata</emphasis>
, inner layers composed of flattened cells of
<emphasis id="E227E02BA1477CB918AF1F050ED1EDE3" italics="true" pageId="0" pageNumber="9">textura angularis</emphasis>
to
<emphasis id="6EA7351B61662C330458585D34EE2B1D" italics="true" pageId="0" pageNumber="9">textura prismatica</emphasis>
.
<emphasis id="E9E6A9A268E455963157620F84340EBB" bold="true" italics="true" pageId="0" pageNumber="9">Conidiophores</emphasis>
reduced to conidiogenous cells.
<emphasis id="54DA1E211C22192F7A285413D39F8E82" bold="true" italics="true" pageId="0" pageNumber="9">Conidiogenous cells</emphasis>
(6.5-)10-16(-25)
<normalizedToken id="F4CD2128E62E9B67D7CA4C5927AA7F1B" originalValue="×">x</normalizedToken>
2-4.5
<normalizedToken id="3FE7CDA3BC4DF42C086295CA39019B82" originalValue="µm">µm</normalizedToken>
(
<emphasis id="B2F9CC12C9C21E2A1E36E20D35BB838C" italics="true" pageId="0" pageNumber="9"></emphasis>
= 13.4
<normalizedToken id="75F15BB9CA97324C6486845E4C2DB5CD" originalValue="×">x</normalizedToken>
3.2
<normalizedToken id="286D72EE50899CC3E18D80C157D15157" originalValue="μm">μm</normalizedToken>
, n = 30), holoblastic to phialidic, hyaline, cylindrical to subcylindrical, terminal or intercalary, septate, smooth-walled, with distinct collarette.
<emphasis id="3250DBEB8CF8758CC50A6CC1A0022747" bold="true" italics="true" pageId="0" pageNumber="9">Conidia</emphasis>
2-3
<normalizedToken id="DE776D4A4A574583CF569A7FA3E42451" originalValue="×">x</normalizedToken>
1.5-2.5
<normalizedToken id="E7611396D36249ACE24A525C66FE69EF" originalValue="µm">µm</normalizedToken>
(
<emphasis id="EA383FE9C1A365CB95B2C712A2CFF2EF" italics="true" pageId="0" pageNumber="9"></emphasis>
= 2.8
<normalizedToken id="F26756320748170F6867B5BAC2A91C92" originalValue="×">x</normalizedToken>
2
<normalizedToken id="F687653C1FF6D2B993906E5D213CF556" originalValue="μm">μm</normalizedToken>
, n = 30) hyaline, ellipsoidal to ovoid, aseptate, smooth-walled, with a guttulate.
</paragraph>
</subSubSection>
<subSubSection id="B59CE037CE457D69AEA6C85A72925AA7" pageId="0" pageNumber="9" type="description">
<paragraph id="1C835603F049D8190647AE9F609E78D0" pageId="0" pageNumber="9">Culture characteristics.</paragraph>
<paragraph id="DBC1890CE76E38CD74FABD20E778AA20" pageId="0" pageNumber="9">Colonies on PDA reaching 25-28 mm diam. after two weeks at room temperature (20-27 °C), medium dense, circular, surface smooth with an entire edge, flattened, slightly raised, fairly fluffy to feathery; from above, initially white, with cream conidial masses, becoming white to cream at the margin, pale yellowish towards the centre with age; from below, white at the margin, dark grey to black towards the centre; pigmentation not produced in PDA. Sporulation in PDA after two weeks, initially visible as cream conidial masses, later forming black conidiomata with hyaline to cream conidial masses on colonies.</paragraph>
</subSubSection>
<subSubSection id="9A96B336F5A4AD964498342E7308A372" pageId="0" pageNumber="9" type="distribution">
<paragraph id="8D8A4CA9EF3375B8E61A0D612BD09E2B" pageId="0" pageNumber="9">Distribution.</paragraph>
<paragraph id="F96B40EFC19AEBA7C8C8FD1DD38705FF" pageId="0" pageNumber="9">China (Yunnan).</paragraph>
</subSubSection>
<subSubSection id="2E4FBD7EEA24924F55E8666B5C6F0554" pageId="0" pageNumber="9" type="specimen examined">
<paragraph id="FA94CD5997B61A74C00EC57B0AD61685" pageId="0" pageNumber="9">Specimen examined.</paragraph>
<paragraph id="C64A00AF7D5D52274998944C9E9471A3" pageId="0" pageNumber="9">
China. Yunnan Province: Xishuangbanna Dai Autonomous Prefecture, Mengla County, Bubeng,
<geoCoordinate id="6A5692544FDA096C1F1C0B934C9DCCBB" degrees="21" direction="north" minutes="36" orientation="latitude" precision="1" seconds="30.13" value="21.60837">21°36'30.13&quot;N</geoCoordinate>
, 101°35'52.54&quot; E, 664 + 5 m a.s.l., on culms of
<taxonomicName id="20145A40680F9AFE5914571F4350FE3C" class="Monocotyledoneae" family="Poaceae" genus="Brachiaria" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Brachiaria mutica" order="Graminales" pageId="0" pageNumber="9" phylum="Angiospermae" rank="species" species="mutica">
<emphasis id="4063DAB3303D44C67D9637136DE43CBD" italics="true" pageId="0" pageNumber="9">Brachiaria mutica</emphasis>
</taxonomicName>
submerged in a freshwater stream, 27 Apr 2021, R. Phookamsak BB21-007 (KUN-HKAS 129041,
<emphasis id="D61ADF904CAF8EDE58A048237AD7B5AD" bold="true" pageId="0" pageNumber="9">holotype</emphasis>
), ex-type living culture, RPC 21-007 = KUNCC 23-14554.
</paragraph>
</subSubSection>
<subSubSection id="A9EC61535B21ED5EB65A4E627D823E59" pageId="0" pageNumber="9" type="notes">
<paragraph id="63C899E6CD1E674937C61FD8CA5A97CE" pageId="0" pageNumber="9">Notes.</paragraph>
<paragraph id="3FBD6838F616A96C65695E7A69B6840D" pageId="0" pageNumber="9">
Based on NCBI nucleotide BLAST search of ITS sequence, the closest hit of
<taxonomicName id="FE3A5A655D40775265B5F33BBE2F09EC" authorityName="Phookamsak, Bhat &amp; Hongsanan" authorityYear="2024" class="Dothideomycetes" family="Dictyosporiaceae" genus="Trichobotrys" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobotrys sinensis" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="species" species="sinensis">
<emphasis id="AAA89D72AB111559E4501ABD8333D263" italics="true" pageId="0" pageNumber="9">Trichobotrys sinensis</emphasis>
</taxonomicName>
(RPC 21-007/ KUNCC 23-14554) is
<taxonomicName id="19B31F998F41A3230E8B56DDD28FD5E6" class="Dothideomycetes" genus="Gregarithecium" kingdom="Fungi" lsidName="Gregarithecium" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="2F6F3ED3A1FAC2D00CD09F61BE54D364" italics="true" pageId="0" pageNumber="9">Gregarithecium</emphasis>
</taxonomicName>
sp. DQD-2016a strain MFLUCC 13-0853 with 99.03% similarity (Identities = 508/513 with 2 gaps) and is similar to
<taxonomicName id="3C0D6AF95E630C690C5BAA369A886DD3" class="Dothideomycetes" family="Dictyosporiaceae" genus="Trichobotrys" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobotrys effusus" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="species" species="effusus">
<emphasis id="5410E309FA5BF0E56E9F387820BAD66A" italics="true" pageId="0" pageNumber="9">Trichobotrys effusus</emphasis>
</taxonomicName>
[as '
<emphasis id="37A01E5DC0BBB8704EDD5F97C52ECFE7" italics="true" pageId="0" pageNumber="9">effusa</emphasis>
'] isolate 1179 (93.51% similarity, Identities = 504/539 with 13 gaps),
<taxonomicName id="5CDA390BC78C710EFFD7CF94B1380EE6" family="Periconiaceae" kingdom="Fungi" lsidName="T. effusus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="effusus">
<emphasis id="2F71869B280C1B934B705458A58F96CF" italics="true" pageId="0" pageNumber="9">T. effusus</emphasis>
</taxonomicName>
[as '
<emphasis id="01EA918D7EDB5CBF4204CA1DDE1E03C8" italics="true" pageId="0" pageNumber="9">effusa</emphasis>
'] strain FS522 (93.35% similarity, Identities = 477/511 with 12 gaps) and
<taxonomicName id="F63806E8EF4281042AC94766FB04D308" family="Periconiaceae" kingdom="Fungi" lsidName="T. effusus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="effusus">
<emphasis id="A6CD0463D5BA8938AB02C602C91DDCAF" italics="true" pageId="0" pageNumber="9">T. effusus</emphasis>
</taxonomicName>
[as '
<emphasis id="921598E3EBD38C54E0F9E5FDD8752498" italics="true" pageId="0" pageNumber="9">effusa</emphasis>
'] isolate HNNUZCJ-94 (93.08% similarity, Identities = 471/506 with 16 gaps). In LSU nucleotide BLAST search, the closest hit of
<taxonomicName id="19022AED44235D687E5E90040363AE67" authorityName="Wu" authorityYear="1977" family="Periconiaceae" kingdom="Fungi" lsidName="T. sinensis" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="sinensis">
<emphasis id="2EA98633E88C2AA3C09E8D73F6C1C69F" italics="true" pageId="0" pageNumber="9">T. sinensis</emphasis>
</taxonomicName>
(RPC 21-007/ KUNCC 23-14554) is
<taxonomicName id="671D0177FF58DEE329037C7AE9BE77B9" class="Dothideomycetes" genus="Gregarithecium" kingdom="Fungi" lsidName="Gregarithecium" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="42BD1FDB7AD1780CC9D696FA4ADA1C49" italics="true" pageId="0" pageNumber="9">Gregarithecium</emphasis>
</taxonomicName>
sp. DQD-2016a strain MFLUCC 13-0853 with 99.88% similarity (Identities = 848/849 with 1 gap) and is similar to
<taxonomicName id="9C5635E6B9D24560435E88DB5A2F2190" class="Dothideomycetes" genus="Gregarithecium" kingdom="Fungi" lsidName="Gregarithecium" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="3E15D1F5BC82F95BE672859EA73EDEF9" italics="true" pageId="0" pageNumber="9">Gregarithecium</emphasis>
</taxonomicName>
sp. isolate L13E (99.40% similarity, Identities = 830/835 with 3 gaps) and
<taxonomicName id="98A1D2BE2A58FABD051D9BF3245B7F64" lsidName="G. curvisporum" pageId="0" pageNumber="9" rank="species" species="curvisporum">
<emphasis id="691F4945AA5D545A5A7191D727E44F88" italics="true" pageId="0" pageNumber="9">G. curvisporum</emphasis>
</taxonomicName>
HHUF 30134 (97.74% similarity, Identities = 822/841 with 5 gaps).
</paragraph>
<paragraph id="AAED15BAC590D05E1C9D4A6899E02C20" pageId="0" pageNumber="9">
Multigene phylogenetic analyses of a concatenated ITS, LSU, SSU and
<emphasis id="E5CE4BF296DE9AF320C6194AE4C75609" italics="true" pageId="0" pageNumber="9">tef1-α</emphasis>
sequence dataset demonstrated that
<taxonomicName id="A6FB2B919A9E560F59B401D29EBCA151" authorityName="Phookamsak, Bhat &amp; Hongsanan" authorityYear="2024" class="Dothideomycetes" family="Dictyosporiaceae" genus="Trichobotrys" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobotrys sinensis" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="species" species="sinensis">
<emphasis id="B49309CB8CBDC6512FCA110C80AD75E4" italics="true" pageId="0" pageNumber="9">Trichobotrys sinensis</emphasis>
</taxonomicName>
(RPC 21-007/ KUNCC 23-14554) shared a branch length with
<taxonomicName id="A904DC7EE70E29C72B06A9D4F9053BE4" class="Dothideomycetes" genus="Gregarithecium" kingdom="Fungi" lsidName="Gregarithecium" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="F3A05ABA05750BDE965D7756DA35155A" italics="true" pageId="0" pageNumber="9">Gregarithecium</emphasis>
</taxonomicName>
sp. DQD-2016a strain MFLUCC 13-0853 and
<taxonomicName id="EB2B3F8D2D4D38AD45019883F4C08BF1" class="Dothideomycetes" genus="Gregarithecium" kingdom="Fungi" lsidName="Gregarithecium" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="E8B2B0A57948819ABABE8C67C8C5F509" italics="true" pageId="0" pageNumber="9">Gregarithecium</emphasis>
</taxonomicName>
sp. isolate GMB 1217 and clustered with the clade of
<taxonomicName id="8A0E4AB95208D029B2E5CF742251BBC8" family="Periconiaceae" kingdom="Fungi" lsidName="T. effusus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="effusus">
<emphasis id="6D6299B6E04E37DA53B888F409964E74" italics="true" pageId="0" pageNumber="9">T. effusus</emphasis>
</taxonomicName>
(Fig.
<figureCitation id="A141676819AAB820C160B4D469749B22" captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Phylogram of the best-scoring ML consensus tree of Trichobotrys species in Dictyosporiaceae and closely-related families viz. Didymosphaeriaceae, Lentitheciaceae, Morosphaeriaceae, Sulcatisporaceae and Trematosphaeriaceae. The new isolate is indicated in blue. Isolates from type materials are in bold. The ML ultrafast bootstrap and Bayesian PP values greater than 70 % and 0.95 are shown at the nodes." figureDoi="10.3897/mycokeys.104.112149.figure2" httpUri="https://binary.pensoft.net/fig/1026393" pageId="0" pageNumber="9">2</figureCitation>
). However,
<taxonomicName id="E872DC5F45E21D004EF386347B381971" class="Dothideomycetes" genus="Gregarithecium" kingdom="Fungi" lsidName="Gregarithecium" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="9AF7993547787ABC77A58C2643A8BDBB" italics="true" pageId="0" pageNumber="9">Gregarithecium</emphasis>
</taxonomicName>
sp. DQD-2016a strain MFLUCC 13-0853 and
<taxonomicName id="26FF5D93C1979686127099A1380F9827" class="Dothideomycetes" genus="Gregarithecium" kingdom="Fungi" lsidName="Gregarithecium" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="FDBC731842CDCB2A3A5B40537D656885" italics="true" pageId="0" pageNumber="9">Gregarithecium</emphasis>
</taxonomicName>
sp. isolate GMB 1217 are unpublished strains. Hence,
<taxonomicName id="78F872B0C68EE0788D94A075F0307CDC" authorityName="Phookamsak, Bhat &amp; Hongsanan" authorityYear="2024" class="Dothideomycetes" family="Dictyosporiaceae" genus="Trichobotrys" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobotrys sinensis" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="species" species="sinensis">
<emphasis id="39804E578B20653A943F853440AA06BE" italics="true" pageId="0" pageNumber="9">Trichobotrys sinensis</emphasis>
</taxonomicName>
(RPC 21-007/ KUNCC 23-14554) is introduced herein as a new species and
<taxonomicName id="E953F12FC687214375BE3EF3ADA788EE" class="Dothideomycetes" genus="Gregarithecium" kingdom="Fungi" lsidName="Gregarithecium" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="7D8BB173A1A2740A6AFDE07C68D23386" italics="true" pageId="0" pageNumber="9">Gregarithecium</emphasis>
</taxonomicName>
sp. (strains MFLUCC 13-0853 and isolate GMB 1217) is re-identified as
<taxonomicName id="CDB5344A153520F23029690ECFBF88F1" authorityName="Wu" authorityYear="1977" family="Periconiaceae" kingdom="Fungi" lsidName="T. sinensis" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="sinensis">
<emphasis id="7CCE2AA4B4A3181E7DEBC7D602818B3D" italics="true" pageId="0" pageNumber="9">T. sinensis</emphasis>
</taxonomicName>
to avoid misidentification. Morphologically,
<taxonomicName id="09B0B1ACADB6933A1B95796B5920684D" authorityName="Wu" authorityYear="1977" family="Periconiaceae" kingdom="Fungi" lsidName="T. sinensis" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="sinensis">
<emphasis id="6C8A8503EA1213E59636ADE5176C584F" italics="true" pageId="0" pageNumber="9">T. sinensis</emphasis>
</taxonomicName>
(RPC 21-007/ KUNCC 23-14554) is typical of
<taxonomicName id="7BBE2A992775032DB74FF81A9D3B71B6" authorityName="Penz. &amp; Sacc., Malpighia 15 (7 - 9): 245 (1902" authorityYear="1901" class="Dothideomycetes" family="Dictyosporiaceae" genus="Trichobotrys" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Trichobotrys" order="Pleosporales" pageId="0" pageNumber="9" phylum="Ascomycota" rank="genus">
<emphasis id="C5C7129D1A62C20A6CFE69C601C7FA00" italics="true" pageId="0" pageNumber="9">Trichobotrys</emphasis>
</taxonomicName>
, but can be distinguished from
<taxonomicName id="FEEBC006909B637963A26F60EC635D75" family="Periconiaceae" kingdom="Fungi" lsidName="T. effusus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="effusus">
<emphasis id="9686A234277FAD5F6BF6509C628D7964" italics="true" pageId="0" pageNumber="9">T. effusus</emphasis>
</taxonomicName>
,
<taxonomicName id="C443D1455E1F0D012710527C1348D1D4" family="Periconiaceae" kingdom="Fungi" lsidName="T. pannosus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="pannosus">
<emphasis id="6F282F1F007FC7FE3C58BF7E498596F0" italics="true" pageId="0" pageNumber="9">T. pannosus</emphasis>
</taxonomicName>
,
<taxonomicName id="739EAAFDBD88CD871278B88C32B5181E" family="Periconiaceae" kingdom="Fungi" lsidName="T. ramosus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="ramosus">
<emphasis id="0610F89FC9DC4EEA82AF56D9ED17EE4B" italics="true" pageId="0" pageNumber="9">T. ramosus</emphasis>
</taxonomicName>
and
<taxonomicName id="C8D378958E8E7947D2FCF77B9F256808" family="Periconiaceae" kingdom="Fungi" lsidName="T. trechisporus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="trechisporus">
<emphasis id="E067B2B2BC3908624F691F12FA62CC11" italics="true" pageId="0" pageNumber="9">T. trechisporus</emphasis>
</taxonomicName>
in having larger conidia (2
<normalizedToken id="3DCB986501B4BD4234ABE339A560BF63" originalValue="µm">µm</normalizedToken>
diam. of
<taxonomicName id="3B602F666AE69A06CC63E1708650E9D6" family="Periconiaceae" kingdom="Fungi" lsidName="T. effusus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="effusus">
<emphasis id="37FD06C30FAB2DB03F56129EF4A61901" italics="true" pageId="0" pageNumber="9">T. effusus</emphasis>
</taxonomicName>
vs. 4
<normalizedToken id="549712DC890366DDE1CAF2F92610BD4A" originalValue="µm">µm</normalizedToken>
diam. of
<taxonomicName id="8CF06EE5D4260454BECC3D9FD9282534" family="Periconiaceae" kingdom="Fungi" lsidName="T. pannosus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="pannosus">
<emphasis id="D19880941753BB1E2517AF96290B6F92" italics="true" pageId="0" pageNumber="9">T. pannosus</emphasis>
</taxonomicName>
vs. 3-5
<normalizedToken id="96C6F51C26C4E0D5F60DEDBD51FD2CD2" originalValue="µm">µm</normalizedToken>
diam. of
<taxonomicName id="08F7BDC8FAA491C12D5C6E0C5EE6E510" family="Periconiaceae" kingdom="Fungi" lsidName="T. ramosus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="ramosus">
<emphasis id="64453829D60444EE53921639AB60068F" italics="true" pageId="0" pageNumber="9">T. ramosus</emphasis>
</taxonomicName>
vs. 5
<normalizedToken id="0D9F877D23D1CD1B5694A41B32C55889" originalValue="×">x</normalizedToken>
3
<normalizedToken id="21B7D50AE251FAB49B96DDB85E8EB529" originalValue="µm">µm</normalizedToken>
or 4
<normalizedToken id="C6F0321834B98A69081AF4DFB6F74208" originalValue="µm">µm</normalizedToken>
diam. of
<taxonomicName id="F53BA4359D5E801705F5C25A70FF1077" family="Periconiaceae" kingdom="Fungi" lsidName="T. trechisporus" order="Pleosporales" pageId="0" pageNumber="9" rank="species" species="trechisporus">
<emphasis id="11AAB8B38BE8D15A0963347BDBDFF0B4" italics="true" pageId="0" pageNumber="9">T. trechisporus</emphasis>
</taxonomicName>
) (
<bibRefCitation id="6F5BA477BD1A378B7D4D7E89F5EC3141" DOI="https://doi.org/10.1111/j.1095-8339.1873.tb00302.x" author="Berkeley, MJ" journalOrPublisher="Botany" pageId="0" pageNumber="9" pagination="65 - 140" refId="B14" refString="Berkeley, MJ, Broome, CE, 1873. Enumeration of the Fungi of Ceylon. Part II., containing the remainder of the Hymenomycetes, with the remaining established tribes of Fungi (Continued.). The Journal of the Linnean Society. Botany 14 (74): 65 - 140, DOI: https://doi.org/10.1111/j.1095-8339.1873.tb00302.x" title="Enumeration of the Fungi of Ceylon. Part II., containing the remainder of the Hymenomycetes, with the remaining established tribes of Fungi (Continued.). The Journal of the Linnean Society." url="https://doi.org/10.1111/j.1095-8339.1873.tb00302.x" volume="14" year="1873">Berkeley and Broome 1873</bibRefCitation>
;
<bibRefCitation id="B37022E982D4F59809E5F795E031F86B" author="Penzig, AGO" journalOrPublisher="Malpighia" pageId="0" pageNumber="9" pagination="201 - 260" refId="B77" refString="Penzig, AGO, Saccardo, PA, 1902. Diagnoses fungorum novorum in insula Java collectorum. Series III. Malpighia 15: 201 - 260" title="Diagnoses fungorum novorum in insula Java collectorum. Series III." volume="15" year="1902">Penzig and Saccardo 1902</bibRefCitation>
;
<bibRefCitation id="5191DE5F00CCFC9A43B6314A2F7E7DA8" DOI="https://doi.org/10.2307/4111564" author="Petch, T" journalOrPublisher="Annals of the Royal Botanic Gardens, Peradeniya" pageId="0" pageNumber="9" pagination="195 - 256" refId="B78" refString="Petch, T, 1917. Additions to Ceylon fungi. Annals of the Royal Botanic Gardens, Peradeniya 6: 195 - 256, DOI: https://doi.org/10.2307/4111564" title="Additions to Ceylon fungi." url="https://doi.org/10.2307/4111564" volume="6" year="1917">Petch 1917</bibRefCitation>
;
<bibRefCitation id="282D32F17C8113FF01B3CBF20D778341" author="Ellis, MB" journalOrPublisher="Commonwealth Mycological Institute, Key Surrey, Kew" pageId="0" pageNumber="9" refId="B31" refString="Ellis, MB, 1971. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute, Key Surrey, Kew" title="Dematiaceous Hyphomycetes." year="1971">Ellis 1971</bibRefCitation>
;
<bibRefCitation id="A12995BC314F83900BB0B45C27DA259C" author="D'Souza, MA" journalOrPublisher="Mycotaxon" pageId="0" pageNumber="9" pagination="105 - 108" refId="B23" refString="D'Souza, MA, Bhat, DJ, 2001. A new species of Trichobotrys from the Western Ghat forests, India. Mycotaxon 80: 105 - 108" title="A new species of Trichobotrys from the Western Ghat forests, India." volume="80" year="2001">
<normalizedToken id="AF39A490818982BFCE456CE81AFC6E3D" originalValue="DSouza">D'Souza</normalizedToken>
and Bhat 2001
</bibRefCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>