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<document id="D6FB9DE27E88FFE98EF4886CAE7E3F40" ID-CLB-Dataset="28219" ID-DOI="http://dx.doi.org/10.3897/mycokeys.47.32085" ID-GBIF-Dataset="66e0ec05-0b6d-4e2d-a436-8e11c2ba2214" ID-PMC="PMC6393397" ID-Pensoft-Pub="1314-4049--35" ID-PubMed="30820166" ModsDocAuthor="" ModsDocDate="2019" ModsDocID="1314-4049--35" ModsDocOrigin="MycoKeys " ModsDocTitle="Tuberpulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula" checkinTime="1553125556611" checkinUser="pensoft" docAuthor="Polemis, Elias, Konstantinidis, Georgios, Fryssouli, Vassiliki, Slavova, Monica, Tsampazis, Triantafyllos, Nakkas, Vasileios, Assyov, Boris, Kaounas, Vasileios &amp; Zervakis, Georgios I." docDate="2019" docId="1223BC53FF05F3E66CE51860A7927763" docLanguage="en" docName="MycoKeys 47: 35-51" docOrigin="MycoKeys 47" docSource="http://dx.doi.org/10.3897/mycokeys.47.32085" docTitle="Tuber pulchrosporum Konstantinidis, Tsampazis, Slavova, Nakkas, Polemis, Fryssouli &amp; Zervakis, sp. nov." docType="treatment" docVersion="7" lastPageNumber="35" masterDocId="FFB46919FFD0FFEB3F74FFEB1C09A52D" masterDocTitle="Tuberpulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula" masterLastPageNumber="51" masterPageNumber="35" pageNumber="35" updateTime="1732886819681" updateUser="ExternalLinkService">
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<mods:title id="874616BBEEE5212A0432A19DF4A6D2C4">Tuberpulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula</mods:title>
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<mods:namePart id="066CB5B1449A7215480D1A53A81A13EF">Polemis, Elias</mods:namePart>
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<mods:namePart id="33D155D5F143733891D4D886640E299E">Konstantinidis, Georgios</mods:namePart>
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<mods:namePart id="65431E7D8176191549C263D47EFF8A6C">Fryssouli, Vassiliki</mods:namePart>
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<mods:namePart id="13BE91475F969EF4786591A4E134EAAB">Slavova, Monica</mods:namePart>
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<mods:namePart id="B53CC1180BB32AB8330CEA4AA0300B50">Tsampazis, Triantafyllos</mods:namePart>
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<mods:namePart id="437C321E4860C1AE852C4723DF33E9E8">Nakkas, Vasileios</mods:namePart>
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<mods:namePart id="EFCDE2D7BC910E5F56D029BD74ADD189">Assyov, Boris</mods:namePart>
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<mods:namePart id="83D6FE0B8A6F7765CFFD4BF6D4C0CCC5">Kaounas, Vasileios</mods:namePart>
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<mods:namePart id="EF62B5D65B39073AB920E91EC0B4F89A">Zervakis, Georgios I.</mods:namePart>
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<mods:typeOfResource id="CA5D24E96B9628438D7365DFA9786DD5">text</mods:typeOfResource>
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<mods:date id="DEED80C5B0220959A68DA4DDBD424F8E">2019</mods:date>
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<mods:classification id="9E241BA4B90B751DCAA15845797B890E">journal article</mods:classification>
<mods:identifier id="5664154AD0BE8FA91EED64A4FB37FA43" type="DOI">http://dx.doi.org/10.3897/mycokeys.47.32085</mods:identifier>
<mods:identifier id="C39BF969679A4DDB6EF7338094054FAE" type="Pensoft-Pub">1314-4049--35</mods:identifier>
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<treatment id="1223BC53FF05F3E66CE51860A7927763" ID-GBIF-Taxon="154473693" LSID="urn:lsid:plazi:treatment:1223BC53FF05F3E66CE51860A7927763" httpUri="http://treatment.plazi.org/id/1223BC53FF05F3E66CE51860A7927763" lastPageNumber="35" pageId="0" pageNumber="35" scope_family="Tuberaceae" scope_order="Pezizales">
<subSubSection id="B6095F34C16783976DD78A07E117FC98" pageId="0" pageNumber="35" type="nomenclature">
<paragraph id="AF4E34FCAAB777EDB686509DAEB7922F" pageId="0" pageNumber="35">
<taxonomicName id="FEA42C6F38FC1A92871CC6B02C10EF43" LSID="MycoBank: MB 828883" authority="Konstantinidis, Tsampazis, Slavova, Nakkas, Polemis, Fryssouli &amp; Zervakis" class="Pezizomycetes" family="Tuberaceae" genus="Tuber" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tuber pulchrosporum" order="Pezizales" pageId="0" pageNumber="35" phylum="Ascomycota" rank="species" species="pulchrosporum">Tuber pulchrosporum Konstantinidis, Tsampazis, Slavova, Nakkas, Polemis, Fryssouli &amp; Zervakis</taxonomicName>
<taxonomicNameLabel id="EF7E3F5F0F438006DED232F2A93AD2F4" pageId="0" pageNumber="35">sp. nov.</taxonomicNameLabel>
Fig. 1a
</paragraph>
</subSubSection>
<subSubSection id="411FF91DF7EF7CB7A488BBF43128286E" pageId="0" pageNumber="35" type="materials_examined">
<paragraph id="5D28BB9AA59C1894697DE4FB77EFC965" pageId="0" pageNumber="35">Type.</paragraph>
<paragraph id="FAB7C672A1554508AB9AD9DBE234ED92" pageId="0" pageNumber="35">
GREECE. Ioannina Prefecture: Ioannina city,
<geoCoordinate id="07AEF8CC075753A7B04248D281061F99" direction="north" orientation="latitude" precision="15" value="39.610832">39°36'39&quot;N</geoCoordinate>
,
<geoCoordinate id="CB788A1616C94702E8D5CE3B3D85CB90" direction="east" orientation="longitude" precision="15" value="20.834723">20°50'05&quot;E</geoCoordinate>
, 500 m alt., in soil under a pure stand of
<taxonomicName id="E33DE65386EEBC780431750502B6F069" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus coccifera" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="species" species="coccifera">Quercus coccifera</taxonomicName>
L., 27 Apr 2016, coll. V. Nakkas, VN091, holotype: ACAM 2016-007 (ACAM!); isotype: SOMF 29980 (SOMF!).
</paragraph>
</subSubSection>
<subSubSection id="21A4CB46EAC25B51E864542C43CB8B49" pageId="0" pageNumber="35" type="diagnosis">
<paragraph id="EEB7166379488BD84B497CD6719F2B9D" pageId="0" pageNumber="35">Diagnosis.</paragraph>
<paragraph id="0BD6539E3AB16D0388E7B408BF7065C6" pageId="0" pageNumber="35">
Ascomata 0.6
<normalizedToken id="2910DBE00A47C0E95609AE7CDDEFAA66" originalValue="7(">-7(-</normalizedToken>
10) cm in diam., subglobose, ovoid to irregularly lobed, usually with shallow basal cavity, surface with fissures and small, dense, almost flat trihedral to polyhedral warts, yellowish-brown to dark brown. Ascospores 25.0-37.0
<normalizedToken id="733185A2E4BB862E7D2F98E24D08575E" originalValue="×">x</normalizedToken>
18.2-25.6
<normalizedToken id="02BA95909AAC3E942521F7991A34FE88" originalValue="μm">μm</normalizedToken>
in (1
<normalizedToken id="4170A1F92559A084C74A8A657644BD36" originalValue=")2">-)2-</normalizedToken>
8-spored asci, ellipsoid to subfusiform on average, Qm=1.4, crested to incompletely reticulate. Hair-like, hyaline to light yellow-brown hyphae protruding from peridium surface.
</paragraph>
<paragraph id="20F7CB2D28B176DA39E8668D9BA8D4C9" pageId="0" pageNumber="35">
<taxonomicName id="A8DB75581DD18FC5C9C81857E3412A07" lsidName="T. panniferum" pageId="0" pageNumber="35" rank="species" species="panniferum">T. panniferum</taxonomicName>
, the closest phylogenetically-related species, produces smaller ascospores (23-26
<normalizedToken id="41937DCFCABB9BEDD90080DAEEA0A6C6" originalValue="×">x</normalizedToken>
18-20
<normalizedToken id="5B1985378B0C183F8BCBB35859E18F24" originalValue="μm">μm</normalizedToken>
), broadly ellipsoid to subglobose on average, with isolated warts; moreover, the peridium surface is woolly-felted due to the presence of dense rusty brown hair-like hyphae.
</paragraph>
</subSubSection>
<subSubSection id="5E954AEAED3C9492A993C96CD332991D" pageId="0" pageNumber="35" type="etymology">
<paragraph id="36395C1D2773B7039657191AFD026175" pageId="0" pageNumber="35">Etymology.</paragraph>
<paragraph id="FD50945085B76C9F62867590752D6558" pageId="0" pageNumber="35">
&quot;
<taxonomicName id="4CD990CF3F5755882FA7516F06ABAAC3" lsidName="pulchrosporum" pageId="0" pageNumber="35" rank="species" species="pulchrosporum">pulchrosporum</taxonomicName>
&quot; refers to the uniquely distinct/impressive ornamentation of the ascospores.
</paragraph>
</subSubSection>
<subSubSection id="B6919B0628220F451ACE694C25638EAB" pageId="0" pageNumber="35" type="description">
<paragraph id="138C54C37E491F383929644A3054B9F8" pageId="0" pageNumber="35">Description.</paragraph>
<paragraph id="0877B2465C656C71DE1BA75746F80E99" pageId="0" pageNumber="35">
Ascomata 0.6
<normalizedToken id="5BD53DB22BA2E2640A93C84249481E1F" originalValue="7(">-7(-</normalizedToken>
10) cm in diameter, tuberous, subglobose, ovoid to irregularly lobed, usually depressed with a shallow - occasionally prominent - basal cavity (excavated), covered up with whitish to yellowish rhizomorphs, fragile, initially greyish to yellowish-brown [fawn (29), sienna (11), fulvous (12)], darkening in maturity to brown [snuff brown (17), umber (18), bay (19), to date brown (24)] or with some shades of purple tinges [purplish date (22), purplish chestnut (21) to brown vinaceous (25)], sometimes with darker black [fuscous black (38)] spots, surface rarely almost smooth, usually rough, with fissures and small, dense, almost flat trihedral to polyhedral warts.
<taxonomicName id="D6D0E293E8006316B15F28B9DAE6E7E8" class="Gastropoda" family="Cymbuliidae" genus="Gleba" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Gleba" order="Pteropoda" pageId="0" pageNumber="35" phylum="Mollusca" rank="genus">Gleba</taxonomicName>
with one of more cavities, initially pinkish-grey [vinaceous buff (31), clay pink (30)], then greyish-brown [milky coffee (28)], yellowish-brown [fulvous (12)], brown [snuff brown (17), umber (18), bay (19)], to purplish-brown in maturity [purplish date (22) to purplish chestnut (21)], with bay (19) to rusty tawny (14) coloured areas close to the cavity, marbled with relatively few and thick white veins, that sometimes are reddening (Fig. 1).
<taxonomicName id="8FE137C255B4C6562AB667E2E45C207E" genus="Odour" lsidName="Odour" pageId="0" pageNumber="35" rank="genus">Odour</taxonomicName>
pleasant truffle-like.
</paragraph>
<caption id="5BB6C389E2DEA8E258E1ADDFECF2C03C" pageId="0" pageNumber="35">
<paragraph id="5F5CABAEE40BA2AF5FCFB4C4DCE36F00" pageId="0" pageNumber="35">
Figure 1.
<taxonomicName id="83D56234B77E47D0DE247640F4D236DA" lsidName="T. pulchrosporum" pageId="0" pageNumber="35" rank="species" species="pulchrosporum">T. pulchrosporum</taxonomicName>
sp. nov.: a ascomata in situ (holotype) b ascomata in situ (paratype) c detail of peridium surface (paratype) d section of peridium (paratype).
</paragraph>
</caption>
<paragraph id="2F1438B74177D4FAC4F9E6E983F5E842" pageId="0" pageNumber="35">
<taxonomicName id="71F6B24D3F8DC43C8B6064BEEC0B365F" class="Magnoliopsida" family="Peraceae" genus="Peridium" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Peridium" order="Malpighiales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Peridium</taxonomicName>
120-370
<normalizedToken id="4D7342AA748FEDE8223A012DB843CBC1" originalValue="μm">μm</normalizedToken>
thick, consisting of two layers; the outer layer 50-160
<normalizedToken id="F3293B1F17B6286080B092818C1E6ECF" originalValue="μm">μm</normalizedToken>
thick, pseudoparenchymatous, composed of yellowish-brown and subglobose inwards to subangular dark brown cells outwards; 4.0-16.3
<normalizedToken id="3B942F95FCB005D55E5C7F2429F3CA9B" originalValue="×">x</normalizedToken>
2.5-13.2
<normalizedToken id="84510BEF94CCE68B86212D40CD741A3F" originalValue="μm">μm</normalizedToken>
, thick-walled (1.5-2.5
<normalizedToken id="D7D181DBDC817603B4776BED7FA686DD" originalValue="μm">μm</normalizedToken>
); the inner layer 70-210
<normalizedToken id="2036683CA1EF570F8B8395D9F29A8AF0" originalValue="µm">µm</normalizedToken>
, composed of pale yellow or hyaline and thick-walled, interwoven hyphae, 2-10
<normalizedToken id="CF2D179390539CD39C3750F3C227C293" originalValue="μm">μm</normalizedToken>
in diameter, forming an intricate texture, becoming agglutinated when dried. Surface with abundant isolated, hyaline to golden-yellow (in water or KOH), thick-walled hair-like hyphae (walls 1.0-1.5
<normalizedToken id="7AC9436B808690D504646B1799A5A8C9" originalValue="μm">μm</normalizedToken>
), 30-140
<normalizedToken id="EF58D0C08E74460521DA53B6BEA7C1CE" originalValue="μm">μm</normalizedToken>
long (occasionally exceeding 300
<normalizedToken id="AA43ABF5ACAC6AC9BB80F120E25F7543" originalValue="μm">μm</normalizedToken>
in Bulgarian specimens) and 2.5-4.5
<normalizedToken id="C3871075BF4BC881B93E66279F25588E" originalValue="μm">μm</normalizedToken>
broad at base, 1-2 septate (Figs 1, 2).
</paragraph>
<caption id="49825E7531E0A01ACB94ED4A6ADD6261" pageId="0" pageNumber="35">
<paragraph id="F6CF6EF778D6290E31CC80B7580DB613" pageId="0" pageNumber="35">
Figure 2.
<taxonomicName id="991E1CCB396488306E86AD6CE25FCD0B" lsidName="T. pulchrosporum" pageId="0" pageNumber="35" rank="species" species="pulchrosporum">T. pulchrosporum</taxonomicName>
sp. nov.: a, b peridium structure c, d hair-like hyphae on peridium surface.
</paragraph>
</caption>
<paragraph id="94817DC7E7645F5291ABA5B6A84CB8FD" pageId="0" pageNumber="35">
Ascospores hyaline when young then yellowish, yellow-brown to brown, at most ellipsoid to subfusiform, some broadly ellipsoid, subglobose to globose, rarely almost limoniform in initial stages, thin-walled and smooth when young, becoming thick-walled at maturity, walls 2
<normalizedToken id="F43EB4C77AF9202D5D191BC7F15DC610" originalValue="3.5(">-3.5(-</normalizedToken>
4)
<normalizedToken id="6E6CDBFCBF02F3062107DD7D2D1F5613" originalValue="μm">μm</normalizedToken>
thick, usually crested to incompletely reticulate, measured (excluding the ornamentation) in the rare 1-spored asci (28-) 46.7
<normalizedToken id="479F669CA04E18EAD214F44B5BDEBCC5" originalValue="±">+/-</normalizedToken>
7.4 (-57)
<normalizedToken id="83CE2FE1E6C9543D07DC0EBFD2D30B96" originalValue="×">x</normalizedToken>
(20-) 29.4
<normalizedToken id="43A4342A1BD6583CE93EB8BCEE304E0B" originalValue="±">+/-</normalizedToken>
4.6 (-34)
<normalizedToken id="27002A2F2E4612D20ECE9F5D7B78ACE5" originalValue="μm">μm</normalizedToken>
, in 2-spored asci (27-) 39.5
<normalizedToken id="06CD4169AFCBA7A8DDEDF2A56C36006A" originalValue="±">+/-</normalizedToken>
5.8 (-53)
<normalizedToken id="A3E15D815774B82878E2E4246DBE4FAC" originalValue="×">x</normalizedToken>
(21-) 27.3
<normalizedToken id="CAD44EB4492C788CC3F6C2A5E5DCBCD9" originalValue="±">+/-</normalizedToken>
4.2 (-41)
<normalizedToken id="6CDA8741A22B11E4FF8C32D5D49A5E7E" originalValue="μm">μm</normalizedToken>
, in 3-spored asci (24-) 34.5
<normalizedToken id="2D9FCDD2CA77D124773D04EE1AF185D6" originalValue="±">+/-</normalizedToken>
5.3 (-49)
<normalizedToken id="E61E6CE40B5DA329C5F12763A687F213" originalValue="×">x</normalizedToken>
(19-) 24.5
<normalizedToken id="9C7D5F34EAE85DCA2EFA3687026970A6" originalValue="±">+/-</normalizedToken>
2.6 (-31)
<normalizedToken id="70E8F928CC5D55DA8C2A2937CCC46A8D" originalValue="μm">μm</normalizedToken>
, in 4-spored (21-) 30.9
<normalizedToken id="FF668F3855B6C23950F39A8791A0DE97" originalValue="±">+/-</normalizedToken>
4.9 (-39)
<normalizedToken id="B6BC99581A1A6BD9227D306A8A9748B4" originalValue="×">x</normalizedToken>
(18-) 22.2
<normalizedToken id="A43CD36409572ACA84C29F5BC394F63C" originalValue="±">+/-</normalizedToken>
2.7 (-30)
<normalizedToken id="34A8DA89245FB0461CA5CB0A248A4738" originalValue="μm">μm</normalizedToken>
, in 5-spored asci (22-) 30.3
<normalizedToken id="6A08C7AEF6BE1DB607118403E02C5CB0" originalValue="±">+/-</normalizedToken>
3.7 (-44)
<normalizedToken id="068346DE5517E8C2C50D6259C7ABB3F0" originalValue="×">x</normalizedToken>
(16-) 21.2
<normalizedToken id="2C3EABB3E09B8C563CDB3D5003702ED5" originalValue="±">+/-</normalizedToken>
2.2 (-28)
<normalizedToken id="F22F82C1CF8686AD500FC009B0D10919" originalValue="μm">μm</normalizedToken>
, in 6-spored asci (22-) 28.9
<normalizedToken id="6DFB1FFE7A99245F86AC9B29E4D03A96" originalValue="±">+/-</normalizedToken>
4.6 (-37)
<normalizedToken id="B2D3AA2B2F9298664F3BD38493D4C699" originalValue="×">x</normalizedToken>
(17-) 20.6
<normalizedToken id="45462343C8A6E12953E4ADC7DE607823" originalValue="±">+/-</normalizedToken>
2.0 (-28)
<normalizedToken id="8423EDDE88FBFA8A648DB72242D38B3C" originalValue="μm">μm</normalizedToken>
, in 7-spored asci (21-) 27.8
<normalizedToken id="864E4FA3BE26DCEA75F06AF5341CC335" originalValue="±">+/-</normalizedToken>
3.3 (-35)
<normalizedToken id="ED9C70A9A6A0C93735B6F57495756DF6" originalValue="×">x</normalizedToken>
(13-) 19.9
<normalizedToken id="2EAC57E91D4161F6B39193549B20D44A" originalValue="±">+/-</normalizedToken>
2.7 (-27)
<normalizedToken id="CDC264A489BC114A9F560E7890219723" originalValue="μm">μm</normalizedToken>
and in 8-spored asci (20-) 25.4
<normalizedToken id="10A1C4CE96173C029C19D79700F379E9" originalValue="±">+/-</normalizedToken>
2.6 (-31)
<normalizedToken id="A56468752927C19F28413CE5CB4719C3" originalValue="×">x</normalizedToken>
(14-) 18.4
<normalizedToken id="BE59F232260949619AAA3DC392BD11E1" originalValue="±">+/-</normalizedToken>
3.1 (-26)
<normalizedToken id="22DB248B7099DC50B4B97B621A9C0CC2" originalValue="μm">μm</normalizedToken>
(Fig. 3); Q=1.0-2.2, Qm=1.43
<normalizedToken id="B5294C63B6F24C454979C671048D4286" originalValue="±">+/-</normalizedToken>
0.19; ornamentation with (0
<normalizedToken id="77323F0C8F14790B8F4E6F4E492917F9" originalValue=")12(">-)1-2(-</normalizedToken>
4) thick veins across the long axis with few to several transverse outgrowths, rarely almost completely reticulate in maturity and then with (0
<normalizedToken id="ED4628607FE4E7F879B4C9C97458E42F" originalValue=")210(">-)2-10(-</normalizedToken>
15) meshes in the longitudinal direction; circumferentially with 22-42 conical warts, with pointed or blunt, straight or curved apices, rarely forked, 1.5
<normalizedToken id="C8CDCD59FE6EFC02050B52101898F3F4" originalValue="6(">-6(-</normalizedToken>
8)
<normalizedToken id="8A1E73E1E9DCD616B277EDDEB16AF226" originalValue="μm">μm</normalizedToken>
tall (Fig. 4); not reacting with
<normalizedToken id="AFD007BC2A39A0F03016B269187A0A84" originalValue="Melzers">Melzer's</normalizedToken>
reagent. Asci (64-) 78-96 (-121)
<normalizedToken id="64EEB7F0B565EA9387F80F43F807B9DE" originalValue="×">x</normalizedToken>
(50-) 65-84 (-98)
<normalizedToken id="E19B2D18D9140076DBE30F4C5C471544" originalValue="μm">μm</normalizedToken>
(excluding stalk), globose, subglobose, ellipsoid, rarely subangular, with a short stalk, 6.5
<normalizedToken id="8AA47F40BCEB83E437942DA1336CCE3E" originalValue="9(">-9(-</normalizedToken>
15)
<normalizedToken id="D28D8FE074A50890988DB664A48D6B04" originalValue="×">x</normalizedToken>
6.5
<normalizedToken id="8857F0B60987903D9618C175ED5CD76B" originalValue="7.5(">-7.5(-</normalizedToken>
10.5)
<normalizedToken id="A09F375EFE0EE7B3240F7BACCE0981E5" originalValue="μm">μm</normalizedToken>
, (1
<normalizedToken id="871949A3C864A4DEA9230E050B2B8813" originalValue=")2">-)2-</normalizedToken>
8-spored (Fig. 3).
</paragraph>
<caption id="7FAE4A389F633D817B23744A256D36C1" pageId="0" pageNumber="35">
<paragraph id="A54CF8B9A7F8945338DE5D549D4AA37D" pageId="0" pageNumber="35">
Figure 3.
<taxonomicName id="94C00B401A013A0D6B9189C00AE41C2A" lsidName="T. pulchrosporum" pageId="0" pageNumber="35" rank="species" species="pulchrosporum">T. pulchrosporum</taxonomicName>
sp. nov.: asci and ascospores.
</paragraph>
</caption>
<caption id="A4D7E531D6ED78A1F4C9EB499A844393" pageId="0" pageNumber="35">
<paragraph id="61D1BD9E849BB748CDB11C5BAAF02787" pageId="0" pageNumber="35">
Figure 4.
<taxonomicName id="AEA80A624FB54A376ABEC40F688237C2" lsidName="T. pulchrosporum" pageId="0" pageNumber="35" rank="species" species="pulchrosporum">T. pulchrosporum</taxonomicName>
sp. nov.: SEM of ascospores.
</paragraph>
</caption>
</subSubSection>
<subSubSection id="288AEE515CEA4D1EC48EACA793E3D1AD" pageId="0" pageNumber="35" type="distribution">
<paragraph id="3DE0368942703991372008E8AB4067E8" pageId="0" pageNumber="35">Distribution and ecology.</paragraph>
<paragraph id="8CEF68A5514828ED9130899E9A6C1FCC" pageId="0" pageNumber="35">
Hypogeous, in soil, appearing solitary or in small groups from March to June, under
<taxonomicName id="C3B06CBA1723D2FB2A990FCB1D02F694" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
sp.,
<taxonomicName id="FF10F2737CC9031223669A6D7E35883F" lsidName="Q. coccifera" pageId="0" pageNumber="35" rank="species" species="coccifera">Q. coccifera</taxonomicName>
or
<taxonomicName id="AA8C7AC50C1CB5A25B56709CC30AB774" lsidName="Q. ilex" pageId="0" pageNumber="35" rank="species" species="ilex">Q. ilex</taxonomicName>
L. or under
<taxonomicName id="67555A0524BE2C7C23D0EBAE1B54ED16" class="Magnoliopsida" family="Betulaceae" genus="Carpinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Carpinus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Carpinus</taxonomicName>
sp. or in mixed stands of
<taxonomicName id="42D1819651D3764428E97B7C4A5BB798" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
sp. and
<taxonomicName id="0E1F7B452CEB1A85DC60BF071F582A4A" class="Pinopsida" family="Pinaceae" genus="Pinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pinus nigra" order="Pinales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="species" species="nigra">Pinus nigra</taxonomicName>
J.F. Arnold or of
<taxonomicName id="D13E6DCA7C40B23F3F4E65555F4FCFA5" lsidName="Q. ilex" pageId="0" pageNumber="35" rank="species" species="ilex">Q. ilex</taxonomicName>
and
<taxonomicName id="DA98E31C7ADA3721EAA15C1CBD3AE463" class="Pinopsida" family="Pinaceae" genus="Pinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Pinus halepensis" order="Pinales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="species" species="halepensis">Pinus halepensis</taxonomicName>
Miller or of
<taxonomicName id="24CFD5CAF4710628A2D2EC1F4831683C" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus robur" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="species" species="robur">Quercus robur</taxonomicName>
L.,
<taxonomicName id="06BB32230F41394444DF9C17E3990DF3" class="Magnoliopsida" family="Betulaceae" genus="Corylus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Corylus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Corylus</taxonomicName>
sp.,
<taxonomicName id="32A7CA0954B9A7EE41FD7B613E7066AB" class="Magnoliopsida" family="Betulaceae" genus="Carpinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Carpinus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Carpinus</taxonomicName>
sp. and
<taxonomicName id="26A20344EC9904659CC59ACA1CE3F7C7" class="Magnoliopsida" family="Sapindaceae" genus="Acer" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Acer" order="Sapindales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Acer</taxonomicName>
sp. It seems to be rather common in continental (northern and central) Greece, while it also occurs in the regions of Eastern Stara Planina and the Black Sea coast of Bulgaria.
</paragraph>
</subSubSection>
<subSubSection id="EA60B8EBB970FB7534543DDA7C8597EF" pageId="0" pageNumber="35" type="additional collections examined (paratypes)">
<paragraph id="11876CF68DD85435C849E046BD1D213F" pageId="0" pageNumber="35">Additional collections examined (paratypes).</paragraph>
<paragraph id="8B48B55AD0C86F13CF1D525F5AEDAB9F" pageId="0" pageNumber="35">
GREECE. Xanthi Prefecture: Toxotes, in soil under a mixed stand dominated by
<taxonomicName id="A1AEBDC80ACA60E1C05481E4EB68D87B" lsidName="Q. coccifera" pageId="0" pageNumber="35" rank="species" species="coccifera">Q. coccifera</taxonomicName>
, 20 June 2008, GK3186b (ACAM 2010-127), coll. P. Panagiotidis. Aitoloakarnania Prefecture: Xiromero, in soil under pure forest of
<taxonomicName id="41E17EBCD4E4EFDCFF62D44CEAC9A260" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
sp., 10 May 2009, GK3801 (ACAM 2010-129), coll. Ch. Chrysopoulos and K. Giatra (GenBank: MK113979); Xiromero, in soil under pure forest of
<taxonomicName id="99185BE62E6D13980BD12D1EA979DDA4" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
sp., 10 May 2009, GK3799 (ACAM 2010-128), coll. Ch. Chrysopoulos and K. Giatra. Trikala Prefecture: Koziakas Mt., in soil under mixed forest of
<taxonomicName id="763B1F1AF8F4F91CB191D38C78DBC368" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
sp. and
<taxonomicName id="7E848AB1728E1D4099BED52FCEF3DB20" lsidName="P. nigra" pageId="0" pageNumber="35" rank="species" species="nigra">P. nigra</taxonomicName>
, 2 April 2013, GK6538 (ACAM 2013-073), coll. K. Papadimitriou (GenBank: MK113978); Koziakas Mt., in soil under mixed forest of
<taxonomicName id="04F417E8387F32A65F31FBA6D398E6F3" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Quercus</taxonomicName>
sp. and
<taxonomicName id="C28AB22276DBBE1F2CC7CD65C52C8083" lsidName="P. nigra" pageId="0" pageNumber="35" rank="species" species="nigra">P. nigra</taxonomicName>
, 2 April 2013, GK6537 (ACAM 2013-074), coll. K. Papadimitriou. Ioannina Prefecture: Metsovo, in soil under pure stand of
<taxonomicName id="2CB958F90C5D59170764E8E32A1C04A6" lsidName="Q. coccifera" pageId="0" pageNumber="35" rank="species" species="coccifera">Q. coccifera</taxonomicName>
, 18 April 2016, GK9408 (ACAM 2016-001), coll. A. Bideris (GenBank: MK113977); Metsovo, in soil under pure stand of
<taxonomicName id="B3D1F55F871AAC66F9A9280C7EF48709" lsidName="Q. coccifera" pageId="0" pageNumber="35" rank="species" species="coccifera">Q. coccifera</taxonomicName>
, 19 April 2016, GK9409 (ACAM 2016-002), coll. A. Bideris; Metsovo, in soil under pure stand of
<taxonomicName id="C248ADB8B1951569C4C26C5735E421F2" lsidName="Q. coccifera" pageId="0" pageNumber="35" rank="species" species="coccifera">Q. coccifera</taxonomicName>
, 19 April 2016, GK9410 (ACAM 2016-003), coll. A. Bideris; Demati, in soil under pure stand of
<taxonomicName id="6D873768B9DB78C903181FA0724CFF22" lsidName="Q. coccifera" pageId="0" pageNumber="35" rank="species" species="coccifera">Q. coccifera</taxonomicName>
, 22 March 2017, GK10231 (ACAM 2017-033), coll. A. Bideris. Attica Prefecture: Katsimidi, in soil under mixed forest of
<taxonomicName id="8C5FA36083D9A6CA2E0A8D254038E16B" lsidName="Q. ilex" pageId="0" pageNumber="35" rank="species" species="ilex">Q. ilex</taxonomicName>
and
<taxonomicName id="FB32F177D0B09FAA53CD87D1CB4DFCDA" lsidName="P. halepensis" pageId="0" pageNumber="35" rank="species" species="halepensis">P. halepensis</taxonomicName>
, 22 March 2016, VK4482 (ACAM 2016-004), coll. V. Kaounas (GenBank: MK113980); Katsimidi, in soil under mixed forest of
<taxonomicName id="4CD041A669F9FF0E9C8F14DBA42E447C" lsidName="Q. ilex" pageId="0" pageNumber="35" rank="species" species="ilex">Q. ilex</taxonomicName>
and
<taxonomicName id="670CBA09BFFC158DFDC011BF634F94AD" lsidName="P. halepensis" pageId="0" pageNumber="35" rank="species" species="halepensis">P. halepensis</taxonomicName>
, 12 April 2016, VK4506 (ACAM 2016-005), coll. V. Kaounas (GenBank: MK113980). Ioannina Prefecture: Neochoropoulo, in soil under a mixed stand of
<taxonomicName id="C9FC78B8A9DE7747BA980C8338436104" lsidName="Q. coccifera" pageId="0" pageNumber="35" rank="species" species="coccifera">Q. coccifera</taxonomicName>
and
<taxonomicName id="80F7287D36C0069490D1C5573478CCCC" lsidName="Q. ilex" pageId="0" pageNumber="35" rank="species" species="ilex">Q. ilex</taxonomicName>
, 27 April 2016, LT1183 (ACAM 2016-006), coll. V. Nakkas (GenBank: MK113976). BULGARIA. Varna, Dolishte village, in soil under pure stand of
<taxonomicName id="FC400F51BED5C1C6B08E108360695512" class="Magnoliopsida" family="Betulaceae" genus="Carpinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Carpinus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Carpinus</taxonomicName>
sp., 07 June 2017, MSL 1945 F8517 (SOMF 29978; ACAM 2017-034), coll. R. Radev (GenBank: MK113981). Sliven, in soil under a mixed stand of
<taxonomicName id="57B3F10FD7232F8F1014F18CEA7884F0" class="Magnoliopsida" family="Fagaceae" genus="Quercus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Quercus robur" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="species" species="robur">Quercus robur</taxonomicName>
,
<taxonomicName id="B876E8917511F2019E170BEB2E95BA9C" class="Magnoliopsida" family="Betulaceae" genus="Corylus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Corylus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Corylus</taxonomicName>
sp.,
<taxonomicName id="E750D2712EF3F367631B559159461DCB" class="Magnoliopsida" family="Betulaceae" genus="Carpinus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Carpinus" order="Fagales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Carpinus</taxonomicName>
sp. and
<taxonomicName id="BF4FE1B3359C00A08A89E6710F22598A" class="Magnoliopsida" family="Sapindaceae" genus="Acer" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Acer" order="Sapindales" pageId="0" pageNumber="35" phylum="Tracheophyta" rank="genus">Acer</taxonomicName>
sp., 09 August 2017, MSL 1961 F0388 (SOMF 29979; ACAM 2017-035), coll. K. Pilasheva &amp; P. Neikov (GenBank: MK113982).
</paragraph>
</subSubSection>
<subSubSection id="ADC06AC87CC2158CE8D4B55208031F7B" pageId="0" pageNumber="35" type="phylogenetic aspects">
<paragraph id="E6FDAE9C90F9A3FA26A85A2FB7391E5B" pageId="0" pageNumber="35">Phylogenetic aspects.</paragraph>
<paragraph id="46C03EA6A4BFE38704FDF7A3A18D8888" pageId="0" pageNumber="35">
The resultant ITS sequence data comprises of 64 sequences which were aligned at 780 sites, 738 of which represent the ITS1-5.8S-ITS2 region, i.e. between the end of the SSU motif (CATTA) and the beginning of LSU motif (TAGGG) (
<bibRefCitation id="882C2B196C60816B53DFAFCCF15F7B05" author="Bonito, G" journalOrPublisher="Molecular Ecology" pageId="0" pageNumber="35" pagination="4994 - 5008" title="A global meta-analysis of Tuber ITS rDNA sequences: species diversity, host associations and long-distance dispersal." url="https://doi.org/10.1111/j.1365-294X.2010.04855.x" volume="19" year="2010 a">Bonito et al. 2010a</bibRefCitation>
). ML and BI analyses yielded similar tree topologies and only the tree inferred from the Bayesian analysis is presented (Fig. 5). The morphologically variable genus
<taxonomicName id="D413C6315D0AF96EE8AA801F56E97428" class="Pezizomycetes" family="Tuberaceae" genus="Tuber" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tuber" order="Pezizales" pageId="0" pageNumber="35" phylum="Ascomycota" rank="genus">Tuber</taxonomicName>
is monophyletic (BS: 100%, PP: 1.00) and several lineages are revealed; for the purposes of this study, the following highly supported clades were included: Aestivum, Excavatum, Gennadii, Gibbosum, Latisporum, Maculatum, Macrosporum, Melanosporum, Puberulum, Regianum, Rufum, Tumericum (=Japonicum).
</paragraph>
<caption id="59011AA38BB5B8AD0544BA7C9D7E54AA" pageId="0" pageNumber="35">
<paragraph id="18F9BAB02E782670BAE652462985F04D" pageId="0" pageNumber="35">
Figure 5. Phylogenetic tree inferred from Bayesian analysis including 62 ITS sequences assigned to 31
<taxonomicName id="8B2E9AF4B9E0974BAE0DC413A6AAE881" class="Pezizomycetes" family="Tuberaceae" genus="Tuber" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Tuber" order="Pezizales" pageId="0" pageNumber="35" phylum="Ascomycota" rank="genus">Tuber</taxonomicName>
taxa, including members of major clades of the genus. Sequences are labelled with Latin binomials, GenBank accession numbers and geographic origin.
<taxonomicName id="40737E4A45382EECA73D218B8680608B" lsidName="T. pulchrosporum" pageId="0" pageNumber="35" rank="species" species="pulchrosporum">T. pulchrosporum</taxonomicName>
sp. nov. is indicated in boldface. Reference sequences deriving from type material are underlined.
<taxonomicName id="C8ED7C4B3948D7A784A73647663E7DB4" class="Pezizomycetes" family="Tuberaceae" genus="Choiromyces" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Choiromyces alveolatus" order="Pezizales" pageId="0" pageNumber="35" phylum="Ascomycota" rank="species" species="alveolatus">Choiromyces alveolatus</taxonomicName>
(
<taxonomicName id="7F7AFB0ECE4F0AB5C6F08027785CCEF5" class="Pezizomycetes" family="Tuberaceae" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="" order="Pezizales" pageId="0" pageNumber="35" phylum="Ascomycota" rank="family">Tuberaceae</taxonomicName>
) was used as the outgroup. Bootstrap (BS) values from Maximum Likelihood (ML) analysis (≥ 70%) and Posterior Probabilities (PPs) from Bayesian Inference (≥ 0.95) are shown at the nodes of branches.
</paragraph>
</caption>
<paragraph id="BCC0D1BDEDC035EBA7A69BE294E2C511" pageId="0" pageNumber="35">
According to the phylogenetic analysis performed,
<taxonomicName id="F9901DC79D4185040FAF8C6A5A6864AC" lsidName="T. pulchrosporum" pageId="0" pageNumber="35" rank="species" species="pulchrosporum">T. pulchrosporum</taxonomicName>
belongs to the Aestivum clade. All eight sequences of this new taxon form a distinct highly supported subclade (BS: 100%, PP: 1.00). Greek specimens possessed almost identical ITS sequences (99.8 - 100%) and so did Bulgarian samples, whereas the comparison between collections from the two countries resulted in sequence identity values of 98.13
<normalizedToken id="B4BA28B2C4061E8A0F99A5EC9F7ACB41" originalValue="±">+/-</normalizedToken>
0.08%. In total, intraspecific sequence identity values for
<taxonomicName id="2C6C1D2582EAD3F85F6D3968BE1351D0" lsidName="T. pulchrosporum" pageId="0" pageNumber="35" rank="species" species="pulchrosporum">T. pulchrosporum</taxonomicName>
exceeded 98% (i.e. 98.05 - 100%). The new species is sister to
<taxonomicName id="612E8DC20275EA1D05FDDE778DCF0AF8" lsidName="T. panniferum" pageId="0" pageNumber="35" rank="species" species="panniferum">T. panniferum</taxonomicName>
(BS: 100%, PP: 1.00); the respective sequences demonstrated low sequence identity (73.21 - 75.08%) further evidencing their distinct taxonomic status.
</paragraph>
</subSubSection>
</treatment>
</document>