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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.34.25974" ID-GBIF-Dataset="125e0951-eb52-4464-8449-200edf801a02" ID-PMC="PMC5993860" ID-Pensoft-Pub="1314-4049-34-63" ID-PubMed="29892206" ModsDocAuthor="" ModsDocDate="2018" ModsDocID="1314-4049-34-63" ModsDocOrigin="MycoKeys 34" ModsDocTitle="New Fusarium species from the Kruger National Park, South Africa" checkinTime="1527871342263" checkinUser="pensoft" docAuthor="Sandoval-Denis, Marcelo, Swart, Wijnand J. &amp; Crous, Pedro W." docDate="2018" docId="436AAAF2068FCB76F182B2DD6746748F" docLanguage="en" docName="MycoKeys 34: 63-92" docOrigin="MycoKeys 34" docSource="http://dx.doi.org/10.3897/mycokeys.34.25974" docTitle="Fusarium fredkrugeri Sandoval-Denis, Crous &amp; W. J. Swart, sp. nov." docType="treatment" docVersion="4" lastPageNumber="63" masterDocId="BF0FFFF3FFD9384BFFCBFFF7FFC7FFCB" masterDocTitle="New Fusarium species from the Kruger National Park, South Africa" masterLastPageNumber="92" masterPageNumber="63" pageNumber="63" updateTime="1668135997706" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>New Fusarium species from the Kruger National Park, South Africa</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Sandoval-Denis, Marcelo</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Swart, Wijnand J.</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Crous, Pedro W.</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2018</mods:date>
<mods:detail type="volume">
<mods:number>34</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>63</mods:start>
<mods:end>92</mods:end>
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<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.34.25974</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.34.25974</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-34-63</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="144212022" LSID="urn:lsid:plazi:treatment:436AAAF2068FCB76F182B2DD6746748F" httpUri="http://treatment.plazi.org/id/436AAAF2068FCB76F182B2DD6746748F" lastPageNumber="63" pageId="0" pageNumber="63">
<subSubSection pageId="0" pageNumber="63" type="nomenclature">
<paragraph pageId="0" pageNumber="63">
<taxonomicName LSID="MB825103" authority="Sandoval-Denis, Crous &amp; W. J. Swart" class="Sordariomycetes" family="Nectriaceae" genus="Fusarium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Fusarium fredkrugeri" order="Hypocreales" pageId="0" pageNumber="63" phylum="Ascomycota" rank="species" species="fredkrugeri">Fusarium fredkrugeri Sandoval-Denis, Crous &amp; W.J. Swart</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="63">sp. nov.</taxonomicNameLabel>
Fig. 6
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="63" type="diagnosis">
<paragraph pageId="0" pageNumber="63">Diagnosis.</paragraph>
<paragraph pageId="0" pageNumber="63">
Differs from
<taxonomicName class="Sordariomycetes" family="Nectriaceae" genus="Fusarium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Fusarium dlaminii" order="Hypocreales" pageId="0" pageNumber="63" phylum="Ascomycota" rank="species" species="dlaminii">Fusarium dlaminii</taxonomicName>
Marasas, P.E. Nelson &amp; Toussoun by producing only one type of aerial conidia, shorter sporodochial conidia and the absence of chlamydospores.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="63" type="materials_examined">
<paragraph pageId="0" pageNumber="63">Type.</paragraph>
<paragraph pageId="0" pageNumber="63">
South Africa, Kruger National Park, Skukuza, Granite Supersite,
<geoCoordinate direction="south" orientation="latitude" precision="1" value="-25.1135">25°06'48.6&quot;S</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="1" value="31.576807">31°34'36.5&quot;E</geoCoordinate>
, from rhizosphere soil of
<taxonomicName class="Magnoliopsida" family="Malvaceae" genus="Melhania" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Melhania acuminata" order="Malvales" pageId="0" pageNumber="63" phylum="Tracheophyta" rank="species" species="acuminata">Melhania acuminata</taxonomicName>
, 23 Mar 2015, W.J. Swart, holotype CBS H-23496, dried culture on OA, culture ex-holotype CBS 144209 = CPC 33747.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="63" type="description">
<paragraph pageId="0" pageNumber="63">Description.</paragraph>
<paragraph pageId="0" pageNumber="63">
Colonies on PDA growing in the dark with an average radial growth rate of 4.7-5.8 mm/d and reaching 22-35 mm diam. in 7 d at 24 °C, filling an entire 9 cm Petri dish in 7 d at 27 and 30 °C. Minimum temperature for growth 12 °C, maximum 36 °C, optimal 27-30 °C. Colony surface at first white to cream coloured, later turning bay to chestnut with pale luteous to luteous periphery; flat, felty to cottony with abundant erect- aerial mycelium forming white patches; colony margins regular and filiform with abundant submerged mycelium. Reverse pale luteous, a blood sepia to chestnut coloured diffusible pigment is scarcely produced at 24 °C, pigment production is markedly enhanced at 27-30 °C, becoming greyish-sepia at 33 °C. Colonies on CMA and OA incubated at 24 °C in the dark reaching 65-67 mm diam. or occupying an entire 9 cm Petri dish in 7 d, respectively. Colony surface pale bay coloured, flat, felty to velvety, aerial mycelium scant, forming white to cream patches; margins regular. Reverse pale bay to pale vinaceous. Sporulation abundant from conidiophores formed on the substrate and aerial mycelium and from sporodochia.
<taxonomicName genus="Conidiophores" lsidName="Conidiophores" pageId="0" pageNumber="63" rank="genus">Conidiophores</taxonomicName>
on the aerial mycelium straight or flexuous, erect or prostrate, septate, smooth- and thin-walled, often appearing rough by accumulation of extracellular material, commonly simple or reduced to conidiogenous cells borne laterally on hyphae or up to 200
<normalizedToken originalValue="μm">μm</normalizedToken>
tall and irregularly branched at various levels, branches bearing lateral and terminal monophialides borne mostly single or in pairs; phialides subulate, ampulliform, lageniform to subcylindrical, smooth- and thin-walled, (8.5
<normalizedToken originalValue=")9.517.5(">-)9.5-17.5(-</normalizedToken>
24.5)
<normalizedToken originalValue="μm">μm</normalizedToken>
long, 2
<normalizedToken originalValue="3(">-3(-</normalizedToken>
3.5)
<normalizedToken originalValue="μm">μm</normalizedToken>
at the widest point, without periclinal thickening, collarets inconspicuous; conidia formed on aerial conidiophores, hyaline, obovoid, ellipsoidal to slightly reniform or allantoid, smooth- and thin-walled, 0-septate, (4.5
<normalizedToken originalValue=")58.5(">-)5-8.5(-</normalizedToken>
12.5)
<normalizedToken originalValue="×">x</normalizedToken>
(1.5
<normalizedToken originalValue=")23.5(">-)2-3.5(-</normalizedToken>
6)
<normalizedToken originalValue="μm">μm</normalizedToken>
, clustering in discrete false heads at the tip of monophialides.
<taxonomicName genus="Sporodochia" lsidName="Sporodochia" pageId="0" pageNumber="63" rank="genus">Sporodochia</taxonomicName>
pale orange to pink coloured, often somewhat translucent, formed abundantly on the surface of carnation leaves and on the agar surface.
<taxonomicName genus="Conidiophores" lsidName="Conidiophores" pageId="0" pageNumber="63" rank="genus">Conidiophores</taxonomicName>
in sporodochia 26-46
<normalizedToken originalValue="μm">μm</normalizedToken>
tall, densely aggregated, irregularly and verticillately branched up to three times, with terminal branches bearing 2-3 monophialides; sporodochial phialides doliiform to subcylindrical, (9
<normalizedToken originalValue=")11.515.5(">-)11.5-15.5(-</normalizedToken>
18.5)
<normalizedToken originalValue="×">x</normalizedToken>
(2.5
<normalizedToken originalValue=")34(">-)3-4(-</normalizedToken>
4.5)
<normalizedToken originalValue="μm">μm</normalizedToken>
, smooth- and thin-walled, with periclinal thickening and an inconspicuous apical collarette. Sporodochial conidia falcate, tapering toward the basal part, robust, moderately curved and slender; apical cell more or less equally sized than the adjacent cell, blunt to slightly papillate; basal cell papillate to distinctly notched, (1
<normalizedToken originalValue=")3">-)3-</normalizedToken>
4-septate, hyaline, thin- and smooth-walled. One-septate conidia: 13
<normalizedToken originalValue="17(">-17(-</normalizedToken>
18)
<normalizedToken originalValue="×">x</normalizedToken>
(2.5
<normalizedToken originalValue=")3">-)3-</normalizedToken>
4
<normalizedToken originalValue="μm">μm</normalizedToken>
; two-septate conidia: 15
<normalizedToken originalValue="×">x</normalizedToken>
4.5
<normalizedToken originalValue="μm">μm</normalizedToken>
; three-septate conidia: (16
<normalizedToken originalValue=")28.539(">-)28.5-39(-</normalizedToken>
45)
<normalizedToken originalValue="×">x</normalizedToken>
(3
<normalizedToken originalValue=")45(">-)4-5(-</normalizedToken>
5.5)
<normalizedToken originalValue="μm">μm</normalizedToken>
; four-septate conidia: 39.5
<normalizedToken originalValue="40(">-40(-</normalizedToken>
41)
<normalizedToken originalValue="×">x</normalizedToken>
4.5-5
<normalizedToken originalValue="μm">μm</normalizedToken>
; overall (13
<normalizedToken originalValue=")27.539.5(">-)27.5-39.5(-</normalizedToken>
45)
<normalizedToken originalValue="×">x</normalizedToken>
(3
<normalizedToken originalValue=")3.5">-)3.5-</normalizedToken>
5.5
<normalizedToken originalValue="μm">μm</normalizedToken>
.
<taxonomicName genus="Chlamydospores" lsidName="Chlamydospores" pageId="0" pageNumber="63" rank="genus">Chlamydospores</taxonomicName>
absent.
</paragraph>
<caption pageId="0" pageNumber="63">
<paragraph pageId="0" pageNumber="63">
Figure 6.
<taxonomicName class="Sordariomycetes" family="Nectriaceae" genus="Fusarium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Fusarium fredkrugeri" order="Hypocreales" pageId="0" pageNumber="63" phylum="Ascomycota" rank="species" species="fredkrugeri">Fusarium fredkrugeri</taxonomicName>
sp. nov.
<normalizedToken originalValue="AD">A-D</normalizedToken>
Colonies on PDA, SNA, OA and CMA, respectively, after 7 d at 24 °C in the dark
<normalizedToken originalValue="EG">E-G</normalizedToken>
<taxonomicName genus="Sporodochia" lsidName="Sporodochia" pageId="0" pageNumber="63" rank="genus">Sporodochia</taxonomicName>
formed on the surface of carnation leaves
<normalizedToken originalValue="HN">H-N</normalizedToken>
Aerial conidiophores, phialides and conidia O, P Aerial conidia Q Sporodochial conidiophores and phialides R Sporodochial conidia. Scale bars: 100
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="EG">E-G</normalizedToken>
); 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="HR">H-R</normalizedToken>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="63" type="distribution">
<paragraph pageId="0" pageNumber="63">Distribution.</paragraph>
<paragraph pageId="0" pageNumber="63">Madagascar, Niger and South Africa.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="63" type="etymology">
<paragraph pageId="0" pageNumber="63">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="63">In honour and memory of Dr. Frederick J. Kruger, pioneer of forest hydrology, fynbos ecology and invasive species and fundamental for the collections included in this study.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="63" type="additional isolates examined">
<paragraph pageId="0" pageNumber="63">Additional isolates examined.</paragraph>
<paragraph pageId="0" pageNumber="63">
Madagascar, from
<taxonomicName class="Magnoliopsida" family="Orobanchaceae" genus="Striga" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Striga hermonthica" order="Lamiales" pageId="0" pageNumber="63" phylum="Tracheophyta" rank="species" species="hermonthica">Striga hermonthica</taxonomicName>
, unknown date, A.A. Abbasher, CBS 144210 = NRRL 26061 = BBA 70127. South Africa, Kruger National Park, Skukuza, Granite Supersite,
<geoCoordinate direction="south" orientation="latitude" precision="1" value="-25.1135">25°06'48.6&quot;S</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="1" value="31.576807">31°34'36.5&quot;E</geoCoordinate>
, from rhizosphere soil of
<taxonomicName class="Magnoliopsida" family="Malvaceae" genus="Melhania" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Melhania acuminata" order="Malvales" pageId="0" pageNumber="63" phylum="Tracheophyta" rank="species" species="acuminata">Melhania acuminata</taxonomicName>
, 23 Mar 2015, W.J. Swart, CBS 144495 = CPC 33746.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="63" type="notes">
<paragraph pageId="0" pageNumber="63">Notes.</paragraph>
<paragraph pageId="0" pageNumber="63">
This species is genetically closely related to
<taxonomicName lsidName="F. dlaminii" pageId="0" pageNumber="63" rank="species" species="dlaminii">F. dlaminii</taxonomicName>
, both species having similar colonial morphology, optimal growth conditions and biogeography. Moreover, both species exhibit relatively short aerial phialides producing conidia in heads, somewhat resembling those produced by
<taxonomicName lsidName="F. oxysporum" pageId="0" pageNumber="63" rank="species" species="oxysporum">F. oxysporum</taxonomicName>
rather than most members of the FFSC (
<bibRefCitation author="Leslie, JF" journalOrPublisher="Phytobiomes" pageId="0" pageNumber="63" url="https://doi.org/10.1002/9780470278376" year="2006">Leslie and Summerell 2006</bibRefCitation>
;
<bibRefCitation author="Marasas, WFO" journalOrPublisher="Mycologia" pageId="0" pageNumber="63" pagination="971 - 975" title="Fusariumdlamini, a new species from Southern Africa." url="https://doi.org/10.2307/3793311" volume="77" year="1985">Marasas et al. 1985</bibRefCitation>
). However, besides exhibiting much faster growth rates,
<taxonomicName lsidName="F. fredkrugeri" pageId="0" pageNumber="63" rank="species" species="fredkrugeri">F. fredkrugeri</taxonomicName>
presents clearly distinctive morphological features such as the production of only one type of aerial conidia (vs. two types in
<taxonomicName lsidName="F. dlaminii" pageId="0" pageNumber="63" rank="species" species="dlaminii">F. dlaminii</taxonomicName>
: allantoid to fusiform and 0-septate; and napiform 0-1-septate); orange to pink sporodochia, produced on carnation leaves but also abundantly on the agar surface (vs. orange sporodochia, produced only on the surface of carnation leaves in
<taxonomicName lsidName="F. dlaminii" pageId="0" pageNumber="63" rank="species" species="dlaminii">F. dlaminii</taxonomicName>
) (
<bibRefCitation author="Leslie, JF" journalOrPublisher="Phytobiomes" pageId="0" pageNumber="63" url="https://doi.org/10.1002/9780470278376" year="2006">Leslie and Summerell 2006</bibRefCitation>
). Additionally,
<taxonomicName lsidName="F. fredkrugeri" pageId="0" pageNumber="63" rank="species" species="fredkrugeri">F. fredkrugeri</taxonomicName>
produces shorter and less septate sporodochial conidia ((1
<normalizedToken originalValue=")3">-)3-</normalizedToken>
4-septate and up to 45
<normalizedToken originalValue="μm">μm</normalizedToken>
long in the latter species vs. mostly 5-septate and up to 54
<normalizedToken originalValue="μm">μm</normalizedToken>
long in
<taxonomicName lsidName="F. dlaminii" pageId="0" pageNumber="63" rank="species" species="dlaminii">F. dlaminii</taxonomicName>
) while chlamydospores are not produced. The latter feature, coupled with the somewhat more complex conidiophores also clearly differentiates
<taxonomicName lsidName="F. fredkrugeri" pageId="0" pageNumber="63" rank="species" species="fredkrugeri">F. fredkrugeri</taxonomicName>
from
<taxonomicName lsidName="F. oxysporum" pageId="0" pageNumber="63" rank="species" species="oxysporum">F. oxysporum</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</document>