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<document id="8DA3F16028126E252ABF98DAEE3B6F64" ID-DOI="10.5281/zenodo.10396362" ID-ISSN="1942-1354" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1707168658757" checkinUser="felipe" docAuthor="Zhang, Jing, Cong, Qian &amp; Grishin, Nick V." docDate="2023" docId="03810139FF9ABB15C0CAFD3EE164B469" docLanguage="en" docName="InsectaMundi.2023.1026.1-115.pdf" docOrigin="Insecta Mundi 2023 (26)" docSource="http://dx.doi.org/10.5281/zenodo.10396362" docStyle="DocumentStyle:F9976E9B7F8A094D890004515A7A0592.3:InsectaMundi.2020-.journal_article" docStyleId="F9976E9B7F8A094D890004515A7A0592" docStyleName="InsectaMundi.2020-.journal_article" docStyleVersion="3" docTitle="Lychnuchus (Enosis) valle Zhang &amp; Cong &amp; Grishin 2023, new species" docType="treatment" docVersion="1" lastPageNumber="68" masterDocId="FFB87941FFDFBB50C00AFF56E355B262" masterDocTitle="Supplementary Materials and Appendix" masterLastPageNumber="115" masterPageNumber="1" pageNumber="68" updateTime="1707169288913" updateUser="GgImagineBatch" zenodo-license-document="CC-BY-NC-3.0">
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<mods:title id="2A449F8E3813C092550EF73D58015370">Supplementary Materials and Appendix</mods:title>
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<mods:namePart id="14B596A849F7B89136480DD0837BE8DB">Zhang, Jing</mods:namePart>
<mods:affiliation id="36DD13C7D105A7B5E976BA161FE771E6">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="16488F0D2D835886D492D12CD000DDE2">Cong, Qian</mods:namePart>
<mods:affiliation id="4F4BE4710EF5C7A9FC5E0ADF496F3BCD">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="38D4603B1725A04E4506FC7A61EA49D8">Grishin, Nick V.</mods:namePart>
<mods:affiliation id="2C761664FA54A9147FB162F0078AEEF0">Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 9050 USA</mods:affiliation>
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<mods:title id="50ED2ABBE6591C8B920C4C440A812F4A">Insecta Mundi</mods:title>
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<mods:date id="7C0B7434B95E0BD0F2E0227A1ED7E0A5">2023</mods:date>
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<emphasis id="B95C6C3DFF9ABB15C0CAFD3EE05BB0E7" bold="true" box="[192,782,616,645]" pageId="69" pageNumber="68">
<taxonomicName id="4C28CBACFF9ABB15C0CAFD3EE138B0E7" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[192,621,616,645]" class="Insecta" family="Hesperiidae" genus="Lychnuchus" kingdom="Animalia" order="Lepidoptera" pageId="69" pageNumber="68" phylum="Arthropoda" rank="species" species="valle" status="sp. nov." subGenus="Enosis">
<emphasis id="B95C6C3DFF9ABB15C0CAFD3EE206B0E7" bold="true" box="[192,339,616,645]" italics="true" pageId="69" pageNumber="68">Lychnuchus</emphasis>
(
<emphasis id="B95C6C3DFF9ABB15C16EFD3FE2E1B0E7" bold="true" box="[356,436,617,645]" italics="true" pageId="69" pageNumber="68">Enosis</emphasis>
)
<emphasis id="B95C6C3DFF9ABB15C1CFFD3EE154B0E7" bold="true" box="[453,513,616,645]" italics="true" pageId="69" pageNumber="68">valle</emphasis>
Grishin
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,
<taxonomicNameLabel id="A26FD146FF9ABB15C273FD3FE05BB0E7" box="[633,782,616,645]" pageId="69" pageNumber="68" rank="species">new species</taxonomicNameLabel>
</emphasis>
</heading>
</paragraph>
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https://zoobank.org/
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<subSubSection id="C332E3A4FF9ABB15C0CAFDF8E148B0AA" box="[192,541,686,712]" pageId="69" pageNumber="68" type="description">
<paragraph id="8B97B02FFF9ABB15C0CAFDF8E148B0AA" blockId="69.[192,941,616,712]" box="[192,541,686,712]" pageId="69" pageNumber="68">
(
<figureCitation id="1313ACAAFF9ABB15C0C2FDF8E252B0AA" box="[200,263,686,712]" captionStart="Figure 7" captionStartId="70.[192,261,1272,1296]" captionTargetBox="[192,1439,282,1242]" captionTargetId="figure-322@70.[192,1440,279,1245]" captionTargetPageId="70" captionText="Figure 7. Phylogenetic trees of Moncina (part 3) and Flagina inferred from protein-coding regions in a) the Z chromosome and b) the mitochondrial genome (in segments). See Fig. 1 legend for other notations." pageId="69" pageNumber="68">Fig. 7</figureCitation>
part, 167168, 396398)
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FF9ABB15C0CAFD81E7D8B6D8" pageId="69" pageNumber="68" type="diagnosis">
<paragraph id="8B97B02FFF9ABB15C0CAFD81E1C9B1BC" blockId="69.[192,1442,727,990]" pageId="69" pageNumber="68">
<emphasis id="B95C6C3DFF9ABB15C0CAFD81E2BCB093" bold="true" box="[192,489,727,754]" pageId="69" pageNumber="68">Definition and diagnosis.</emphasis>
Phylogenetic trees reveal that
<specimenCount id="9D2E7BA6FF9ABB15C334FD8EE096B093" box="[830,963,727,754]" count="2" pageId="69" pageNumber="68" type="female">two females</specimenCount>
from eastern
<collectingCountry id="F33FF0BFFF9ABB15C46AFD81E79AB093" box="[1120,1231,727,753]" name="Colombia" pageId="69" pageNumber="68">Colombia</collectingCountry>
identified as
<taxonomicName id="4C28CBACFF9ABB15C56FFD81E10EB171" authority="(Nicolay, 1980)" baseAuthorityName="Nicolay" baseAuthorityYear="1980" class="Insecta" family="Hesperiidae" genus="Lychnuchus" kingdom="Animalia" order="Lepidoptera" pageId="69" pageNumber="68" phylum="Arthropoda" rank="species" species="topo" subGenus="Enosis">
<emphasis id="B95C6C3DFF9ABB15C56FFD81E25BB171" italics="true" pageId="69" pageNumber="68">Lychnuchus</emphasis>
(
<emphasis id="B95C6C3DFF9ABB15C12AFDACE230B171" box="[288,357,762,787]" italics="true" pageId="69" pageNumber="68">Enosis</emphasis>
)
<emphasis id="B95C6C3DFF9ABB15C17DFDACE2F2B171" box="[375,423,762,787]" italics="true" pageId="69" pageNumber="68">topo</emphasis>
(Nicolay, 1980)
</taxonomicName>
(
<typeStatus id="54930E8DFF9ABB15C261FDAFE1C8B171" box="[619,669,761,787]" pageId="69" pageNumber="68">type</typeStatus>
locality in
<collectingCountry id="F33FF0BFFF9ABB15C314FDAFE02AB171" box="[798,895,761,787]" name="Ecuador" pageId="69" pageNumber="68">Ecuador</collectingCountry>
) show prominent genetic differentiation from it (
<figureCitation id="1313ACAAFF9ABB15C0C0FC4DE258B157" box="[202,269,795,821]" captionStart="Figure 7" captionStartId="70.[192,261,1272,1296]" captionTargetBox="[192,1439,282,1242]" captionTargetId="figure-322@70.[192,1440,279,1245]" captionTargetPageId="70" captionText="Figure 7. Phylogenetic trees of Moncina (part 3) and Flagina inferred from protein-coding regions in a) the Z chromosome and b) the mitochondrial genome (in segments). See Fig. 1 legend for other notations." pageId="69" pageNumber="68">Fig. 7</figureCitation>
): e.g., their COI barcodes differ by 5.8% (38 bp), and therefore represent a new species. This new species differs from
<taxonomicName id="4C28CBACFF9ABB15C145FC6BE2CEB134" baseAuthorityName="Nicolay" baseAuthorityYear="1980" box="[335,411,829,854]" class="Insecta" family="Hesperiidae" genus="Lychnuchus" kingdom="Animalia" order="Lepidoptera" pageId="69" pageNumber="68" phylum="Arthropoda" rank="species" species="topo">
<emphasis id="B95C6C3DFF9ABB15C145FC6BE2CEB134" box="[335,411,829,854]" italics="true" pageId="69" pageNumber="68">L. topo</emphasis>
</taxonomicName>
, which is known only from males, by vestigial or lacking pale dot on the ventral hindwing and darker tornal area of the ventral forewing. Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly320.2.13:T495C, aly1603.33.3:G145A, aly770.42.5:A69T, aly
<date id="FF9696EFFF9ABB15C376FCF7E0B4B1DE" box="[892,993,929,956]" pageId="69" pageNumber="68" value="2096-02-03">2096.2.3</date>
:G151C, aly3370.5.2:C48A, and COI barcode: T10C, T64C, T451C, A535T, T574C.
</paragraph>
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<emphasis id="B95C6C3DFF9ABB15C0CAFCBAE11BB664" bold="true" box="[192,590,1004,1030]" pageId="69" pageNumber="68">
Barcode sequence of the
<typeStatus id="54930E8DFF9ABB15C1EAFCBAE11CB664" box="[480,585,1004,1030]" pageId="69" pageNumber="68" type="holotype">holotype</typeStatus>
.
</emphasis>
Sample NVG-19021G09, GenBank OR837698, 658 base pairs: AACTTTATACTTTATTTTTGGAATTTGAGCAGGAATATTAGGAACTTCTTTAAGATTATTAATCCGTACAGAACTAGGTAATCCAGGTTCTTTAATT GGAGATGATCAAATTTATAATACTATTGTTACAGCCCATGCTTTTATTATAATTTTCTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATT GATTAGTTCCTTTAATATTAGGAGCACCTGATATAGCTTTCCCACGAATAAATAACATAAGATTTTGAATATTACCTCCCTCTTTAATGTTATTGAC TTCAAGAAGTATCGTAGAAAATGGTGCAGGTACAGGATGAACAGTTTATCCTCCTTTATCTTCTAATATTGCTCATCAAGGTTCTTCTGTTGACTTA GCTATTTTTTCCTTACATTTAGCAGGAATTTCATCTATTTTAGGAGCTATTAATTTTATTACCACAATTATTAATATACGAATTAAAAATATATCAT TTGATCAAATACCTTTATTTGTTTGATCAGTAGGTATTACAGCCTTACTTTTACTTTTATCTTTACCTGTTTTAGCTGGAGCTATTACCATACTTCT AACTGATCGAAATTTAAATACTTCATTTTTTGATCCAGCCGGAGGAGGAGACCCTATTTTATACCAACATTTATTT
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FF9ABB15C0CAFB9AE771B7DA" pageId="69" pageNumber="68" type="materials_examined">
<paragraph id="8B97B02FFF9ABB15C0CAFB9AE79EB7EC" blockId="69.[191,1440,1228,1422]" pageId="69" pageNumber="68">
<emphasis id="B95C6C3DFF9ABB15C0CAFB9AE239B684" bold="true" box="[192,364,1228,1254]" pageId="69" pageNumber="68">Type material.</emphasis>
<materialsCitation id="3B40BA72FF9ABB15C17FFB9AE0D7B70F" collectingDate="1989-01-29" collectionCode="DC, USNM, V" collectorName="Valle &amp; J. B. Sullivan &amp; Genit. Vial &amp; Nick &amp; Grishin &amp; QR Code" country="Colombia" elevation="1800" location="National Museum of Natural History" municipality="Smithsonian Institution" pageId="69" pageNumber="68" specimenCount="2" specimenCount-female="2" stateProvince="Washington" typeStatus="holotype">
<emphasis id="B95C6C3DFF9ABB15C17FFB9AE2BCB684" bold="true" box="[373,489,1228,1254]" pageId="69" pageNumber="68">
<typeStatus id="54930E8DFF9ABB15C17FFB9AE2B1B684" box="[373,484,1228,1254]" pageId="69" pageNumber="68" type="holotype">Holotype</typeStatus>
:
</emphasis>
<specimenCount id="9D2E7BA6FF9ABB15C1F9FB9BE15DB68B" box="[499,520,1229,1257]" count="1" pageId="69" pageNumber="68" type="female"></specimenCount>
deposited in the
<location id="8EF7E6F4FF9ABB15C2DFFB9AE72AB684" LSID="urn:lsid:plazi:treatment:03810139FF9ABB15C0CAFD3EE164B469:8EF7E6F4FF9ABB15C2DFFB9AE72AB684" box="[725,1151,1228,1254]" country="Colombia" municipality="Smithsonian Institution" name="National Museum of Natural History" pageId="69" pageNumber="68" stateProvince="Washington">National Museum of Natural History</location>
,
<collectingMunicipality id="6BF32A55FF9ABB15C486FB9AE6C9B684" box="[1164,1436,1228,1254]" pageId="69" pageNumber="68">Smithsonian Institution</collectingMunicipality>
,
<collectingRegion id="49EC7ECDFF9ABB15C0CAFBBBE21CB765" box="[192,329,1261,1287]" country="United States of America" name="Washington" pageId="69" pageNumber="68">Washington</collectingRegion>
,
<collectionCode id="ED3928EAFF9ABB15C15DFBB8E2D4B76A" box="[343,385,1262,1288]" pageId="69" pageNumber="68">DC</collectionCode>
, USA (
<collectionCode id="ED3928EAFF9ABB15C1DCFBB8E17EB76A" box="[470,555,1262,1288]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871" lsid="urn:lsid:biocol.org:col:34871" name="Smithsonian Institution, National Museum of Natural History" pageId="69" pageNumber="68" type="Museum">USNM</collectionCode>
), illustrated in
<figureCitation id="1313ACAAFF9ABB15C2D7FBBBE027B765" box="[733,882,1261,1287]" captionStart="Figures 149172" captionStartId="102.[192,271,1810,1834]" captionTargetBox="[198,1437,224,1779]" captionTargetId="figure-87@102.[192,1440,221,1781]" captionTargetPageId="102" captionText="Figures 149172. Holotypes, data in text. 149150) Amblyscirtes (Flor) meridus sp. n. 151152) Rectava chiriquensis sp. n. 153154) Cobalopsis adictys sp. n. 155156) Cymaenes melaporphyrus sp. n. 157158) Lerema (Morys) ecuadorica sp. n. 159160) Saturnus obscurior sp. n. 161162) Cantha zoirodicta sp. n. 163164) Cantha meiodicta sp. n. 165166) Phlebodes duplex sp. n. 167168) Lychnuchus (Enosis) valle sp. n. 169170) Eutychide ochoides sp. n. 171172) Dion bora sp. n." pageId="69" pageNumber="68">Fig. 167168</figureCitation>
, bears the following five rectangular labels, four white: [
<date id="FF9696EFFF9ABB15C110FA59E2F4B74B" box="[282,417,1295,1321]" pageId="69" pageNumber="68" value="1989-01-29">
<collectingDate id="EFD26F07FF9ABB15C110FA59E2F4B74B" box="[282,417,1295,1321]" pageId="69" pageNumber="68" value="1989-01-29">Jan.29, 1989</collectingDate>
</date>
| Carret del Mar | to Dapa Rd.,
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</quantity>
|
<collectorName id="26DDD5F9FF9ABB15C368FA59E0C9B74B" box="[866,924,1295,1321]" pageId="69" pageNumber="68">Valle</collectorName>
,
<collectingCountry id="F33FF0BFFF9ABB15C3ACFA59E740B74B" box="[934,1045,1295,1321]" name="Colombia" pageId="69" pageNumber="68">Colombia</collectingCountry>
|
<collectorName id="26DDD5F9FF9ABB15C422FA46E7E6B74B" box="[1064,1203,1295,1322]" pageId="69" pageNumber="68">J. B. Sullivan</collectorName>
], [
<collectorName id="26DDD5F9FF9ABB15C4D4FA59E603B74B" box="[1246,1366,1295,1321]" pageId="69" pageNumber="68">Genit. Vial</collectorName>
| SRS-3248], [DNA sample ID: | NVG-19021G09 | c/o
<collectorName id="26DDD5F9FF9ABB15C2E2FA67E048B729" box="[744,797,1329,1355]" pageId="69" pageNumber="68">Nick</collectorName>
<collectionCode id="ED3928EAFF9ABB15C32EFA67E062B729" box="[804,823,1329,1355]" country="Canada" lsid="urn:lsid:biocol.org:col:13946" name="Royal British Columbia Museum - Herbarium" pageId="69" pageNumber="68" type="Museum">V</collectionCode>
.
<collectorName id="26DDD5F9FF9ABB15C34AFA67E0C3B729" box="[832,918,1329,1355]" pageId="69" pageNumber="68">Grishin</collectorName>
], [USNMENT | {
<collectorName id="26DDD5F9FF9ABB15C46EFA67E784B729" box="[1124,1233,1329,1355]" pageId="69" pageNumber="68">QR Code</collectorName>
} | 01532704], and one red [
<typeStatus id="54930E8DFF9ABB15C123FA04E2EFB70E" box="[297,442,1362,1388]" pageId="69" pageNumber="68" type="holotype">HOLOTYPE</typeStatus>
<specimenCount id="9D2E7BA6FF9ABB15C1B5FA02E281B712" box="[447,468,1364,1392]" count="1" pageId="69" pageNumber="68" type="female"></specimenCount>
|
<taxonomicName id="4C28CBACFF9ABB15C1EDFA04E19AB70E" box="[487,719,1362,1388]" class="Insecta" family="Hesperiidae" genus="Lychnuchus" kingdom="Animalia" order="Lepidoptera" pageId="69" pageNumber="68" phylum="Arthropoda" rank="subGenus" subGenus="Enosis">Lychnuchus (Enosis)</taxonomicName>
|
<taxonomicName id="4C28CBACFF9ABB15C2E8FA04E024B70E" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[738,881,1362,1388]" class="Insecta" family="Hesperiidae" genus="Lychnuchus" kingdom="Animalia" order="Lepidoptera" pageId="69" pageNumber="68" phylum="Arthropoda" rank="species" species="valle">valle Grishin</taxonomicName>
]
</materialsCitation>
.
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<typeStatus id="54930E8DFF9ABB15C386FA04E0A3B70E" box="[908,1014,1362,1388]" pageId="69" pageNumber="68" type="paratype">Paratype</typeStatus>
:
</emphasis>
<specimenCount id="9D2E7BA6FF9ABB15C40BFA04E776B712" box="[1025,1059,1362,1392]" count="1" pageId="69" pageNumber="68" type="female">1♀</specimenCount>
NVG-22035H06
<collectingCountry id="F33FF0BFFF9ABB15C4E2FA04E60FB70E" box="[1256,1370,1362,1388]" name="Colombia" pageId="69" pageNumber="68">Colombia</collectingCountry>
: Valle, San Antonio, above Cali,
<quantity id="4CD01DCAFF9ABB15C1D0FA22E17AB7EC" box="[474,559,1396,1422]" metricMagnitude="3" metricUnit="m" metricValue="1.9" pageId="69" pageNumber="68" unit="m" value="1900.0">1900 m</quantity>
,
<date id="FF9696EFFF9ABB15C233FA22E1EEB7EC" box="[569,699,1396,1422]" pageId="69" pageNumber="68" value="1987-02-02">2-Feb-1987</date>
, J. B. Sullivan leg., genitalia SRS-3224 [
<collectionCode id="ED3928EAFF9ABB15C462FA22E795B7EC" box="[1128,1216,1396,1422]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871" lsid="urn:lsid:biocol.org:col:34871" name="Smithsonian Institution, National Museum of Natural History" pageId="69" pageNumber="68" type="Museum">USNM</collectionCode>
].
</paragraph>
<paragraph id="8B97B02FFF9ABB15C0CAFAC8E771B7DA" blockId="69.[192,1060,1437,1464]" box="[192,1060,1437,1464]" pageId="69" pageNumber="68">
<emphasis id="B95C6C3DFF9ABB15C0CAFAC8E20EB7D5" bold="true" box="[192,347,1437,1464]" pageId="69" pageNumber="68">
<typeStatus id="54930E8DFF9ABB15C0CAFAC8E3ACB7DA" box="[192,249,1438,1464]" pageId="69" pageNumber="68">Type</typeStatus>
locality.
</emphasis>
<collectingCountry id="F33FF0BFFF9ABB15C16BFACBE286B7D5" box="[353,467,1437,1463]" name="Colombia" pageId="69" pageNumber="68">Colombia</collectingCountry>
: Valle, Carret del Mar to Dapa Rd., elevation
<quantity id="4CD01DCAFF9ABB15C3C0FAC8E775B7DA" box="[970,1056,1438,1464]" metricMagnitude="3" metricUnit="m" metricValue="1.8" pageId="69" pageNumber="68" unit="m" value="1800.0">1800 m</quantity>
.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FF9ABB15C0CAFA91E0BBB783" box="[192,1006,1479,1506]" pageId="69" pageNumber="68" type="etymology">
<paragraph id="8B97B02FFF9ABB15C0CAFA91E0BBB783" blockId="69.[192,1006,1479,1506]" box="[192,1006,1479,1506]" pageId="69" pageNumber="68">
<emphasis id="B95C6C3DFF9ABB15C0CAFA91E211B783" bold="true" box="[192,324,1479,1505]" pageId="69" pageNumber="68">Etymology.</emphasis>
The name is for the
<typeStatus id="54930E8DFF9ABB15C222FA9EE10DB780" box="[552,600,1480,1506]" pageId="69" pageNumber="68">type</typeStatus>
locality and is a noun in apposition.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FF9ABB15C0CAFAA6E164B469" box="[192,561,1520,1547]" pageId="69" pageNumber="68" type="distribution">
<paragraph id="8B97B02FFF9ABB15C0CAFAA6E164B469" blockId="69.[192,561,1520,1547]" box="[192,561,1520,1547]" pageId="69" pageNumber="68">
<emphasis id="B95C6C3DFF9ABB15C0CAFAA6E20EB468" bold="true" box="[192,347,1520,1546]" pageId="69" pageNumber="68">Distribution.</emphasis>
Eastern
<collectingCountry id="F33FF0BFFF9ABB15C1B6FAA7E17BB469" box="[444,558,1521,1547]" name="Colombia" pageId="69" pageNumber="68">Colombia</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
</document>