359 lines
27 KiB
XML
359 lines
27 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.94.95751" ID-Pensoft-Pub="1314-4049-94-51" ID-Pensoft-UUID="1E443200E6585C66B1D669737794361F" ModsDocID="1314-4049-94-51" checkinTime="1670952471273" checkinUser="pensoft" docAuthor="Lu, Li, Karunarathna, Samantha C., Dai, Dong-qin, Jayawardena, Ruvishika S., Suwannarach, Nakarin & Tibpromma, Saowaluck" docDate="2022" docId="EB69069BB814520BBFD44E61265EAB85" docLanguage="en" docName="MycoKeys 94: 51-71" docOrigin="MycoKeys 94" docPubDate="2022-12-12" docSource="http://dx.doi.org/10.3897/mycokeys.94.95751" docTitle="Nigrograna coffeae L. Lu & Tibpromma 2022, sp. nov." docType="treatment" docVersion="1" id="1E443200E6585C66B1D669737794361F" lastPageNumber="51" masterDocId="1E443200E6585C66B1D669737794361F" masterDocTitle="Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China" masterLastPageNumber="71" masterPageNumber="51" pageNumber="51" updateTime="1670952471273" updateUser="pensoft">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Lu, Li</mods:namePart>
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<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China & Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Karunarathna, Samantha C.</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-7080-0781</mods:nameIdentifier>
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<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Dai, Dong-qin</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0001-8935-8807</mods:nameIdentifier>
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<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Jayawardena, Ruvishika S.</mods:namePart>
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<mods:affiliation>Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Suwannarach, Nakarin</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-2653-1913</mods:nameIdentifier>
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<mods:affiliation>School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
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<mods:nameIdentifier type="email">suwan.462@gmail.com</mods:nameIdentifier>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Tibpromma, Saowaluck</mods:namePart>
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<mods:affiliation>Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China</mods:affiliation>
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<mods:nameIdentifier type="email">saowaluckfai@gmail.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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||
<mods:part>
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<mods:date>2022</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2022-12-12</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>94</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>51</mods:start>
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<mods:end>71</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.94.95751</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.94.95751</mods:identifier>
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||
<mods:identifier type="Pensoft-Pub">1314-4049-94-51</mods:identifier>
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||
<mods:identifier type="Pensoft-UUID">1E443200E6585C66B1D669737794361F</mods:identifier>
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||
</mods:mods>
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<treatment LSID="urn:lsid:plazi:treatment:EB69069BB814520BBFD44E61265EAB85" httpUri="http://treatment.plazi.org/id/EB69069BB814520BBFD44E61265EAB85" lastPageNumber="51" pageId="0" pageNumber="51">
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<subSubSection pageId="0" pageNumber="51" type="nomenclature">
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<paragraph pageId="0" pageNumber="51">
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<taxonomicName LSID="EB69069B-B814-520B-BFD4-4E61265EAB85" authority="L. Lu & Tibpromma" authorityName="L. Lu & Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Biatriosporaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna coffeae" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="coffeae" status="sp. nov.">Nigrograna coffeae L. Lu & Tibpromma</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="51">sp. nov.</taxonomicNameLabel>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="description">
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<paragraph pageId="0" pageNumber="51">
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Nigrograna coffeae (ZHKU 22 - 0121, holotype) a, b ascomata on the host substrate c a vertical section through an ascoma d peridium e hamathecium f-k asci l germinated ascospore m culture on pda from above and reverse n-s ascospores (arrows indicate the septa). Scale bars: 50 μm (c); 10 μm (d-l); 5 μm (n-s)." figureDoi="10.3897/mycokeys.94.95751.figure2" httpUri="https://binary.pensoft.net/fig/781089" pageId="0" pageNumber="51">Fig. 2</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="etymology">
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<paragraph pageId="0" pageNumber="51">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="51">
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Species epithet refers to the host genus "
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<taxonomicName class="Magnoliopsida" family="Rubiaceae" genus="Coffea" kingdom="Plantae" lsidName="Coffea" order="Gentianales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="51">Coffea</emphasis>
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</taxonomicName>
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" where the fungus was isolated.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="holotype">
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<paragraph pageId="0" pageNumber="51">Holotype.</paragraph>
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<paragraph pageId="0" pageNumber="51">ZHKU 22-0121.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="description">
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<paragraph pageId="0" pageNumber="51">Description.</paragraph>
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<paragraph pageId="0" pageNumber="51">
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Saprobic</emphasis>
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on decaying branch of
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<taxonomicName class="Magnoliopsida" family="Rubiaceae" genus="Coffea" kingdom="Plantae" lsidName="Coffea arabica" order="Gentianales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="arabica">
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<emphasis italics="true" pageId="0" pageNumber="51">Coffea arabica</emphasis>
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</taxonomicName>
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.
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<emphasis bold="true" pageId="0" pageNumber="51">
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Sexual morph:
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<emphasis italics="true" pageId="0" pageNumber="51">Ascomata</emphasis>
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</emphasis>
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90-140
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<normalizedToken originalValue="µm">µm</normalizedToken>
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high, 140-200
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<normalizedToken originalValue="μm">μm</normalizedToken>
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wide (x̄ = 115
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<normalizedToken originalValue="×">x</normalizedToken>
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168
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 10), immersed, solitary, black spots on substrate, subglobose to oval, sometimes obpyriform, some with ostiolate.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Peridium</emphasis>
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10-15
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<normalizedToken originalValue="µm">µm</normalizedToken>
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wide, composed of 3-5 layers, hyaline to brown (#937463) cells of
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">textura angularis</emphasis>
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.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Hamathecium</emphasis>
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1.5-3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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wide, composed of numerous, hyaline, filamentous, septate, branched, pseudoparaphyses.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Asci</emphasis>
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50-70
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<normalizedToken originalValue="×">x</normalizedToken>
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7-11
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(x̄ = 58
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<normalizedToken originalValue="×">x</normalizedToken>
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9
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20), 8-spored, bitunicate, fissitunicate, clavate to cylindric-clavate, short stalked, some with club-shape pedicel, apically rounded, with a small ocular chamber.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="51">Ascospores</emphasis>
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12-16
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<normalizedToken originalValue="×">x</normalizedToken>
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4-5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, (x̄ = 14.4
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<normalizedToken originalValue="×">x</normalizedToken>
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4.6
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 30), overlapping uni- to bi-seriately arranged, fusiform, straight or slightly curved, hyaline when immature and become pale brown (#e1af33) to dark-brown (#6e5031) when mature, mostly 1-septate, few 2 or 3-septate, constricted at each septum, with obviously guttulate.
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<emphasis bold="true" pageId="0" pageNumber="51">Asexual morph</emphasis>
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: Undetermined.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="description">
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<paragraph pageId="0" pageNumber="51">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="51">Ascospores germinated on PDA within 24 h and germ tubes arising from both ends. Colonies on PDA, reaching 4.5 cm diam. after two months of incubation at room temperature (22-26 °C), initially white (#f2f3f4) becoming grey (#bbbeb2) to dark brown (#6e5031) at maturity, dense, circular, slightly raised, smooth surface, radially fimbriate at the edge, reverse dark green (#3a4543) to brown (#937463).</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="materials_examined">
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<paragraph pageId="0" pageNumber="51">Material examined.</paragraph>
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<paragraph pageId="0" pageNumber="51">
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<materialsCitation accessionNumber="OP450967, OP450973, OP432243, OP450981, OP432247, OP450968, OP450974, OP432244, OP450982, OP432248" collectingDate="2021-09-15" country="China" elevation="857" latitude="22.521666" location="Pu'wen Town" longLatPrecision="21" longitude="101.045555" municipality="Xishuangbanna" specimenCount="1" stateProvince="Yunnan Province" typeStatus="holotype">
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<location LSID="urn:lsid:plazi:treatment:EB69069BB814520BBFD44E61265EAB85:D36D49A164203289C0CA1238491DBA46" country="China" latitude="22.521666" longLatPrecision="21" longitude="101.045555" municipality="Xishuangbanna" name="Pu'wen Town" stateProvince="Yunnan Province">
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<normalizedToken originalValue="Pu’wen">Pu'wen</normalizedToken>
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Town
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</location>
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,
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<collectingMunicipality>Xishuangbanna</collectingMunicipality>
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,
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<collectingRegion country="China" name="Yunnan">Yunnan Province</collectingRegion>
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,
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<collectingCountry name="China">China</collectingCountry>
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, on a decaying branch of
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<taxonomicName class="Magnoliopsida" family="Rubiaceae" genus="Coffea" kingdom="Plantae" lsidName="Coffea arabica" order="Gentianales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="arabica">
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<emphasis italics="true" pageId="0" pageNumber="51">Coffea arabica</emphasis>
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</taxonomicName>
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, (
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<geoCoordinate degrees="22" direction="north" minutes="31" orientation="latitude" precision="15" seconds="18" value="22.521666">22°31'18"N</geoCoordinate>
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,
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<geoCoordinate degrees="101" direction="east" minutes="2" orientation="longitude" precision="15" seconds="44" value="101.045555">101°2'44"E</geoCoordinate>
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,
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<quantity metricMagnitude="2" metricUnit="m" metricValue="8.5689" unit="m" value="856.89">
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<elevation metricMagnitude="2" metricUnit="m" metricValue="8.5689" unit="m" value="856.89">856.89 m</elevation>
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</quantity>
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),
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<collectingDate value="2021-09-15">15 September 2021</collectingDate>
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, LiLu, JHPW16 (ZHKU 22-0121,
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<typeStatus>holotype</typeStatus>
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), ZHKUCC 22-0210 = ZHKUCC 22-0211. GenBank number; ITS:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450967">OP450967</accessionNumber>
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, LSU:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450973">OP450973</accessionNumber>
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,
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<emphasis italics="true" pageId="0" pageNumber="51">rpb</emphasis>
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2:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432243">OP432243</accessionNumber>
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, SSU:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450981">OP450981</accessionNumber>
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,
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<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
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1-α:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432247">OP432247</accessionNumber>
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(ZHKUCC 22-0210, ex-type); ITS:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450968">OP450968</accessionNumber>
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, LSU:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450974">OP450974</accessionNumber>
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,
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<emphasis italics="true" pageId="0" pageNumber="51">rpb</emphasis>
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2:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432244">OP432244</accessionNumber>
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, SSU:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP450982">OP450982</accessionNumber>
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,
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<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
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1-α:
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<accessionNumber httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/OP432248">OP432248</accessionNumber>
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(ZHKUCC 22-0211)
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</materialsCitation>
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.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="51" type="notes">
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<paragraph pageId="0" pageNumber="51">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="51">
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Our phylogenetic analyses showed that
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<taxonomicName authorityName="L. Lu & Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna coffeae" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="coffeae">
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<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna coffeae</emphasis>
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</taxonomicName>
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forms an independent clade (100% ML, 1.00 BIPP, Fig.
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. The maximum-likelihood phylogram of Nigrograna based on a combined SSU, LSU, ITS, rpb 2 and tef 1 - α sequence dataset with Cyclothyriella rubronotata CBS 141486 and CBS 419.85 as the outgroup taxa (Dayarathne et al. 2020). The maximum-likelihood bootstrap values (ML ≥ 60 %, left) and Bayesian Inference Posterior Probability values (BIPP ≥ 0.90, right) are shown above the nodes. Strains derived from the current study are in blue, while type strains are in bold." figureDoi="10.3897/mycokeys.94.95751.figure1" httpUri="https://binary.pensoft.net/fig/781088" pageId="0" pageNumber="51">1</figureCitation>
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), and is phylogenetically related to
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<taxonomicName lsidName="N. yasuniana" pageId="0" pageNumber="51" rank="species" species="yasuniana">
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<emphasis italics="true" pageId="0" pageNumber="51">N. yasuniana</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="N. jinghongensis" pageId="0" pageNumber="51" rank="species" species="jinghongensis">
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<emphasis italics="true" pageId="0" pageNumber="51">N. jinghongensis</emphasis>
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</taxonomicName>
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.
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<taxonomicName class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna yasuniana" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="yasuniana">
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<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna yasuniana</emphasis>
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</taxonomicName>
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was reported as endophytes from
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<taxonomicName class="Magnoliopsida" family="Euphorbiaceae" genus="Conceveiba" kingdom="Plantae" lsidName="Conceveiba guianensis" order="Malpighiales" pageId="0" pageNumber="51" phylum="Tracheophyta" rank="species" species="guianensis">
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<emphasis italics="true" pageId="0" pageNumber="51">Conceveiba guianensis</emphasis>
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</taxonomicName>
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Aubl. in Ecuador, but there were not enough morphological data, the comparison of base pairs in ITS showed 3.4% differences (15/433 bp), LSU showed 1.5% differences (12/812bp), SSU only showed 0.3% differences (3/1028 bp),
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<emphasis italics="true" pageId="0" pageNumber="51">rpb</emphasis>
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2 showed 14% differences (117/829 bp), and
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<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
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1-α showed 3.2% differences (31/954 bp) (
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<bibRefCitation DOI="https://doi.org/10.1007/s00606-016-1350-2" author="Kolarik, M" journalOrPublisher="Plant Systematics and Evolution" pageId="0" pageNumber="51" pagination="35 - 50" refId="B23" refString="Kolarik, M, Spakowicz, DJ, Gazis, R, Shaw, J, Kubatova, A, Novakova, A, Chudickova, M, Forcina, GC, Kang, KW, Kelnarova, I, Skaltsas, D, 2017. Biatriospora (Ascomycota: Pleosporales) is an ecologically diverse genus including facultative marine fungi and endophytes with biotechnological potential. Plant Systematics and Evolution 303 (1): 35 - 50, DOI: https://doi.org/10.1007/s00606-016-1350-2" title="Biatriospora (Ascomycota: Pleosporales) is an ecologically diverse genus including facultative marine fungi and endophytes with biotechnological potential." url="https://doi.org/10.1007/s00606-016-1350-2" volume="303" year="2017">
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<normalizedToken originalValue="Kolařík">Kolarik</normalizedToken>
|
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et al. 2017
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||
</bibRefCitation>
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).
|
||
<taxonomicName authorityName="Wanas. & K.D.Hyde" authorityYear="2021" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna jinghongensis" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="jinghongensis">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna jinghongensis</emphasis>
|
||
</taxonomicName>
|
||
was introduced as a saprobic fungus from woody litter in China, and our new isolate shares a similar size (12-16
|
||
<normalizedToken originalValue="×">x</normalizedToken>
|
||
4-5
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
<emphasis italics="true" pageId="0" pageNumber="51">vs</emphasis>
|
||
12-15
|
||
<normalizedToken originalValue="×">x</normalizedToken>
|
||
4-5.5
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
) and color (hyaline to dark brown
|
||
<emphasis italics="true" pageId="0" pageNumber="51">vs</emphasis>
|
||
yellowish-brown to brown) of ascospores with
|
||
<taxonomicName lsidName="N. jinghongensis" pageId="0" pageNumber="51" rank="species" species="jinghongensis">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. jinghongensis</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<bibRefCitation DOI="https://doi.org/10.1007/s13225-021-00489-3" author="Boonmee, S" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="51" pagination="1 - 335" refId="B5" refString="Boonmee, S, Wanasinghe, DN, Calabon, MS, Huanraluek, N, Chandrasiri, SK, Jones, GE, Rossi, W, Leonardi, M, Singh, SK, Rana, S, Singh, PN, Maurya, DK, Lagashetti, AC, Choudhary, D, Dai, YC, Zhao, CL, Mu, YH, Yuan, HS, He, SH, Phookamsak, R, Jiang, HB, Martin, MP, Duenas, M, Telleria, MT, Kalucka, LK, Jagodzinski, AM, Liimatainen, K, Pereira, DS, Phillips, AJL, Suwannarach, N, Kumla, J, Khuna, S, Lumyong, S, Potter, TB, Shivas, RG, Sparks, AH, Vaghefi, N, Abdel-Wahab, MA, Abdel-Aziz, FA, Li, GJ, Lin, WF, Singh, U, Bhatt, RP, Lee, HB, Nguyen, TTT, Kirk, PM, Dutta, AK, Acharya, K, Sarma, VV, Niranjan, M, Rajeshkumar, KC, Ashtekar, N, Lad, S, Wijayawardene, NN, Bhat, DJ, Xu, RJ, Wijesinghe, SN, Shen, HW, Luo, ZL, Zhang, JY, Sysouphanthong, P, Thongklang, N, Bao, DF, Aluthmuhandiram, JVS, Abdollahzadeh, J, Javadi, A, Dovana, F, Usman, M, Khalid, AN, Dissanayake, AJ, Telagathoti, A, Probst, M, Peintner, U, Garrido-Benavent, I, Bona, L, Merenyi, Z, Boros, L, Zoltan, B, Stielow, JB, Jiang, N, Tian, CM, Shams, E, Dehghanizadeh, F, Pordel, A, Javan-Nikkhah, M, Denchev, TT, Denchev, CM, Kemler, M, Begerow, D, Deng, CY, Harrower, E, Bozorov, T, Kholmuradova, T, Gafforov, Y, Abdurazakov, A, Xu, JC, Mortimer, PE, Ren, GC, Jeewon, R, Maharachchikumbura, SSN, Phukhamsakda, C, Mapook, A, Hyde, KD, 2021. Fungal diversity notes 1387-1511: Taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 111: 1 - 335, DOI: https://doi.org/10.1007/s13225-021-00489-3" title="Fungal diversity notes 1387 - 1511: Taxonomic and phylogenetic contributions on genera and species of fungal taxa." url="https://doi.org/10.1007/s13225-021-00489-3" volume="111" year="2021">Boonmee et al. 2021</bibRefCitation>
|
||
), but there are some significant differences in the size of the ascomata (90-140
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
high, 140-200
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
wide
|
||
<emphasis italics="true" pageId="0" pageNumber="51">vs</emphasis>
|
||
300-400
|
||
<normalizedToken originalValue="µm">µm</normalizedToken>
|
||
high 220-300
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
wide) and the shape of ascospores (fusiform, straight or slightly curved
|
||
<emphasis italics="true" pageId="0" pageNumber="51">vs</emphasis>
|
||
ellipsoid) (
|
||
<bibRefCitation DOI="https://doi.org/10.1007/s13225-021-00489-3" author="Boonmee, S" journalOrPublisher="Fungal Diversity" pageId="0" pageNumber="51" pagination="1 - 335" refId="B5" refString="Boonmee, S, Wanasinghe, DN, Calabon, MS, Huanraluek, N, Chandrasiri, SK, Jones, GE, Rossi, W, Leonardi, M, Singh, SK, Rana, S, Singh, PN, Maurya, DK, Lagashetti, AC, Choudhary, D, Dai, YC, Zhao, CL, Mu, YH, Yuan, HS, He, SH, Phookamsak, R, Jiang, HB, Martin, MP, Duenas, M, Telleria, MT, Kalucka, LK, Jagodzinski, AM, Liimatainen, K, Pereira, DS, Phillips, AJL, Suwannarach, N, Kumla, J, Khuna, S, Lumyong, S, Potter, TB, Shivas, RG, Sparks, AH, Vaghefi, N, Abdel-Wahab, MA, Abdel-Aziz, FA, Li, GJ, Lin, WF, Singh, U, Bhatt, RP, Lee, HB, Nguyen, TTT, Kirk, PM, Dutta, AK, Acharya, K, Sarma, VV, Niranjan, M, Rajeshkumar, KC, Ashtekar, N, Lad, S, Wijayawardene, NN, Bhat, DJ, Xu, RJ, Wijesinghe, SN, Shen, HW, Luo, ZL, Zhang, JY, Sysouphanthong, P, Thongklang, N, Bao, DF, Aluthmuhandiram, JVS, Abdollahzadeh, J, Javadi, A, Dovana, F, Usman, M, Khalid, AN, Dissanayake, AJ, Telagathoti, A, Probst, M, Peintner, U, Garrido-Benavent, I, Bona, L, Merenyi, Z, Boros, L, Zoltan, B, Stielow, JB, Jiang, N, Tian, CM, Shams, E, Dehghanizadeh, F, Pordel, A, Javan-Nikkhah, M, Denchev, TT, Denchev, CM, Kemler, M, Begerow, D, Deng, CY, Harrower, E, Bozorov, T, Kholmuradova, T, Gafforov, Y, Abdurazakov, A, Xu, JC, Mortimer, PE, Ren, GC, Jeewon, R, Maharachchikumbura, SSN, Phukhamsakda, C, Mapook, A, Hyde, KD, 2021. Fungal diversity notes 1387-1511: Taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 111: 1 - 335, DOI: https://doi.org/10.1007/s13225-021-00489-3" title="Fungal diversity notes 1387 - 1511: Taxonomic and phylogenetic contributions on genera and species of fungal taxa." url="https://doi.org/10.1007/s13225-021-00489-3" volume="111" year="2021">Boonmee et al. 2021</bibRefCitation>
|
||
). Based on the sequence blast results, ITS, LSU and
|
||
<emphasis italics="true" pageId="0" pageNumber="51">rpb</emphasis>
|
||
2 gene sequences were similar to
|
||
<taxonomicName authorityName="J.de Gruyter, G.J.M.Verkley & P.W.Crous" authorityYear="2012" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="genus">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna</emphasis>
|
||
</taxonomicName>
|
||
sp., with 97.5% (MZ270683), 98.4% (MK762716), and 86% (MZ508421) respectively, SSU was similar to
|
||
<taxonomicName lsidName="N. mycophila" pageId="0" pageNumber="51" rank="species" species="mycophila">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. mycophila</emphasis>
|
||
</taxonomicName>
|
||
with 99% (KX650510), and
|
||
<emphasis italics="true" pageId="0" pageNumber="51">tef</emphasis>
|
||
1-α was similar to
|
||
<taxonomicName lsidName="N. yasuniana" pageId="0" pageNumber="51" rank="species" species="yasuniana">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. yasuniana</emphasis>
|
||
</taxonomicName>
|
||
with 96.6% (LN626670). Therefore, we introduce our new isolate as a new species
|
||
<taxonomicName lsidName="N. coffeae" pageId="0" pageNumber="51" rank="species" species="coffeae">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">N. coffeae</emphasis>
|
||
</taxonomicName>
|
||
based on both morphological characteristics and phylogenetic analyses.
|
||
</paragraph>
|
||
<caption doi="10.3897/mycokeys.94.95751.figure2" httpUri="https://binary.pensoft.net/fig/781089" pageId="0" pageNumber="51" start="Figure 2" startId="F2">
|
||
<paragraph pageId="0" pageNumber="51">
|
||
<emphasis bold="true" pageId="0" pageNumber="51">Figure 2.</emphasis>
|
||
<taxonomicName authorityName="L. Lu & Tibpromma" authorityYear="2022" class="Dothideomycetes" family="Nigrogranaceae" genus="Nigrograna" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Nigrograna coffeae" order="Pleosporales" pageId="0" pageNumber="51" phylum="Ascomycota" rank="species" species="coffeae">
|
||
<emphasis italics="true" pageId="0" pageNumber="51">Nigrograna coffeae</emphasis>
|
||
</taxonomicName>
|
||
(ZHKU 22-0121, holotype)
|
||
<emphasis bold="true" pageId="0" pageNumber="51">a, b</emphasis>
|
||
ascomata on the host substrate
|
||
<emphasis bold="true" pageId="0" pageNumber="51">c</emphasis>
|
||
a vertical section through an ascoma
|
||
<emphasis bold="true" pageId="0" pageNumber="51">d</emphasis>
|
||
peridium
|
||
<emphasis bold="true" pageId="0" pageNumber="51">e</emphasis>
|
||
hamathecium
|
||
<emphasis bold="true" pageId="0" pageNumber="51">f-k</emphasis>
|
||
asci
|
||
<emphasis bold="true" pageId="0" pageNumber="51">l</emphasis>
|
||
germinated ascospore
|
||
<emphasis bold="true" pageId="0" pageNumber="51">m</emphasis>
|
||
culture on pda from above and reverse
|
||
<emphasis bold="true" pageId="0" pageNumber="51">n-s</emphasis>
|
||
ascospores (arrows indicate the septa). Scale bars: 50
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">c</emphasis>
|
||
); 10
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">d-l</emphasis>
|
||
); 5
|
||
<normalizedToken originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis bold="true" pageId="0" pageNumber="51">n-s</emphasis>
|
||
).
|
||
</paragraph>
|
||
</caption>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |