269 lines
36 KiB
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269 lines
36 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/phytokeys.227.103890" ID-Pensoft-Pub="1314-2003-227-99" ID-Pensoft-UUID="03A0DB8123885CDFA16EEA1583274595" ModsDocID="1314-2003-227-99" checkinTime="1685804682949" checkinUser="pensoft" docAuthor="Du, Fei-Chao, Li, Yu-Hang & Xu, Kui-Dong" docDate="2023" docId="B2483978A8C55523A3D705CE762E04B3" docLanguage="en" docName="PhytoKeys 227: 99-108" docOrigin="PhytoKeys 227" docPubDate="2023-06-02" docSource="http://dx.doi.org/10.3897/phytokeys.227.103890" docTitle="Pleurosigma pacificum Du & Li & Xu 2023, sp. nov." docType="treatment" docVersion="1" id="03A0DB8123885CDFA16EEA1583274595" lastPageNumber="99" masterDocId="03A0DB8123885CDFA16EEA1583274595" masterDocTitle="Morphology and molecular phylogeny of Pleurosigma pacificum sp. nov. (Pleurosigmataceae), a new tropical pelagic species from the Western Pacific Ocean" masterLastPageNumber="108" masterPageNumber="99" pageNumber="99" updateTime="1685804682949" updateUser="pensoft">
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<mods:title id="2BE75E4B9DA5635E53AF56418FCFA48C">Morphology and molecular phylogeny of Pleurosigma pacificum sp. nov. (Pleurosigmataceae), a new tropical pelagic species from the Western Pacific Ocean</mods:title>
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<mods:name id="BC876C4E87268329DC55221CCBA997ED" type="personal">
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<mods:roleTerm id="AFF6902841651355E19D7B8C359D03AD">Author</mods:roleTerm>
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<mods:namePart id="5ECC4FC8409DD04130846D47376E4616">Du, Fei-Chao</mods:namePart>
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<mods:nameIdentifier id="8F11A6CD52A60BB637DA35CFC89EA4AF" type="ORCID">https://orcid.org/0009-0005-0674-5761</mods:nameIdentifier>
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<mods:affiliation id="ECD6076BCCA3629F104D59B591A28748">Laboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China & University of Chinese Academy of Sciences, Beijing 100049, China</mods:affiliation>
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<mods:namePart id="1FB9C1E24F0512C0D7F340E790EB1CBD">Li, Yu-Hang</mods:namePart>
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<mods:nameIdentifier id="F38970CD4272DBD8BAC0714DC17DF417" type="ORCID">https://orcid.org/0000-0002-5546-1774</mods:nameIdentifier>
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<mods:affiliation id="D52916EBA088AE30B3320EC019DA17D1">Laboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China</mods:affiliation>
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<mods:namePart id="B563967D69C33DDCCBF93BF149377AFB">Xu, Kui-Dong</mods:namePart>
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<mods:nameIdentifier id="BFA7576A917CC62431408E1E30B584CB" type="ORCID">https://orcid.org/0000-0002-5186-519X</mods:nameIdentifier>
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<mods:affiliation id="A3FB25C6E61B6E68BDDE57C6D3232A13">Laboratory of Marine Organism Taxonomy and Phylogeny, Qingdao Key Laboratory of Marine Biodiversity and Conservation, Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China & University of Chinese Academy of Sciences, Beijing 100049, China & Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China</mods:affiliation>
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<mods:nameIdentifier id="814639EAB18F69E1F4E139E9B14337E4" type="email">kxu@qdio.ac.cn</mods:nameIdentifier>
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<mods:title id="D0E2DA0014E423881E27DE8B6253351D">PhytoKeys</mods:title>
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<mods:date id="B002085BA705713259861D3F304525AD">2023</mods:date>
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<mods:number id="54BCCAA0F0183BD06496DA03164E5AB4">2023-06-02</mods:number>
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<mods:number id="5E84DB5779A32782850FA397D3A572F0">227</mods:number>
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<mods:url id="A4F1BF17F4AEA982135B92B811585BC8">http://dx.doi.org/10.3897/phytokeys.227.103890</mods:url>
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<mods:classification id="51816A38C879256F989B8FBD03CE6108">journal article</mods:classification>
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<mods:identifier id="0F734D240533C6A116E871844E4D2A9C" type="DOI">http://dx.doi.org/10.3897/phytokeys.227.103890</mods:identifier>
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<treatment id="B2483978A8C55523A3D705CE762E04B3" LSID="urn:lsid:plazi:treatment:B2483978A8C55523A3D705CE762E04B3" httpUri="http://treatment.plazi.org/id/B2483978A8C55523A3D705CE762E04B3" lastPageNumber="99" pageId="0" pageNumber="99">
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<subSubSection id="A44AC75A2E1CEF0EF70789527CACAE5A" pageId="0" pageNumber="99" type="nomenclature">
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<paragraph id="52BBE0ED746F48C524F8CE3B46AF71BE" pageId="0" pageNumber="99">
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<taxonomicName id="FA92F6E9C1346A3F93B4A5E14FCF76F9" LSID="B2483978-A8C5-5523-A3D7-05CE762E04B3" authority="Du & Li & Xu, 2023" authorityName="Du & Li & Xu" authorityYear="2023" class="Bacillariophyceae" family="Pleurosigmataceae" genus="Pleurosigma" higherTaxonomySource="treatment-meta" kingdom="Plantae" lsidName="Pleurosigma pacificum" order="Naviculales" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="pacificum" status="sp. nov.">Pleurosigma pacificum</taxonomicName>
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<taxonomicNameLabel id="14F4EA64F0187427A2FB72B4ADF28682" pageId="0" pageNumber="99">sp. nov.</taxonomicNameLabel>
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</paragraph>
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</subSubSection>
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<subSubSection id="8C4853EF214E439D0CEF71AB287B15C5" pageId="0" pageNumber="99" type="description">
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<paragraph id="D6F3263F3AFB448631DCCC226A506FDF" pageId="0" pageNumber="99">
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<figureCitation id="CE518B9FA8591B3ECFBC1C99FB37549B" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">Fig. 1</figureCitation>
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</paragraph>
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||
</subSubSection>
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<subSubSection id="E2051EA22EA52280A98759A47D215D9B" pageId="0" pageNumber="99" type="description">
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<paragraph id="1B47D77E65F39357E1841A493E129460" pageId="0" pageNumber="99">Description.</paragraph>
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<paragraph id="B102DDB7721D6F45BE0B454B28875688" pageId="0" pageNumber="99">
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Valves lanceolate, gradually tapering towards the subacute ends, 45.0-51.5
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<normalizedToken id="00D6A86CC7328528F7BE4BBB30893C53" originalValue="µm">µm</normalizedToken>
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long and 13.0-15.6
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<normalizedToken id="B89D353F0841E1D88CC0DF5C961D79A1" originalValue="µm">µm</normalizedToken>
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wide (Fig.
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<figureCitation id="85CE04D8CD2C7D81919919A4E9D61F81" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1A-D</figureCitation>
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). Raphe filiform, straight, slightly curved near poles (Fig.
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<figureCitation id="B4BD3EA4BB6B20C03EF238583624C4D9" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1A-D</figureCitation>
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). Raphe angle +2° to +4°. Valve center roundish (Fig.
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<figureCitation id="5B031A69BE3913662FBB9D392A8CB4FD" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1E</figureCitation>
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, arrowhead). Terminal area funnel-shaped (Fig.
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<figureCitation id="A69AB9BEB5CC613DFC50F11D2523AEA1" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1F</figureCitation>
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). Transverse striae straight, parallel throughout, 21-22 in 10
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<normalizedToken id="18F8D954D45F775D031F5FE023708F9F" originalValue="µm">µm</normalizedToken>
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, oblique striae 21-23 in 10
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<normalizedToken id="8EF3DEB5290195A4537AA223B411EF92" originalValue="µm">µm</normalizedToken>
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, intersecting at an angle between 32° to 35°.
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</paragraph>
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<caption id="4D4C997E4CF1707A1D76A05BC10A533D" doi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99" start="Figure 1" startId="F1">
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<paragraph id="6917A92B8B26060A6EA6CA0CE7F53B3A" pageId="0" pageNumber="99">
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<emphasis id="55720CD1A303C953980E79C9BF16F723" bold="true" pageId="0" pageNumber="99">Figure 1.</emphasis>
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<emphasis id="76F1A8775DB746EEFE7F8E07AF72344B" bold="true" pageId="0" pageNumber="99">A-F</emphasis>
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LM photographs of
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<taxonomicName id="F2E276E14FBAD354D4DAABE71FDA992C" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. pacificum" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="pacificum">
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<emphasis id="0008036AAF6A054BED057250BF600146" italics="true" pageId="0" pageNumber="99">P. pacificum</emphasis>
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</taxonomicName>
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<emphasis id="CCE6452D6A7E4424D47E8D6B25187D7B" bold="true" pageId="0" pageNumber="99">A-D</emphasis>
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cleaned frustules showing parallel transverse striae under LM
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<emphasis id="E603FE174ADD492F299CC98EAB6ADF72" bold="true" pageId="0" pageNumber="99">E</emphasis>
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valve center showing a roundish central area (arrowhead)
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<emphasis id="EEB724CE5F75DC04B4262C5F01CC392D" bold="true" pageId="0" pageNumber="99">F</emphasis>
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funnel-shaped terminal area in apical position
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<emphasis id="2A3904B5FEA32CE69F7EFC3A264D7337" bold="true" pageId="0" pageNumber="99">G-L</emphasis>
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SEM photographs of
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<taxonomicName id="1C53A707C9E07DFB835EFB6580CF6033" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. pacificum" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="pacificum">
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<emphasis id="C0EEE18E80E9117721AA11B9198882B3" italics="true" pageId="0" pageNumber="99">P. pacificum</emphasis>
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</taxonomicName>
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<emphasis id="BDDAB0ECD0CD80BAD61CCED2EDC7007E" bold="true" pageId="0" pageNumber="99">G</emphasis>
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internal view of the whole valve
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<emphasis id="A96254393B908885D84D4F1E33E0E0DE" bold="true" pageId="0" pageNumber="99">H</emphasis>
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internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars
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<emphasis id="ED92BB8E0855562C030F16825149CA62" bold="true" pageId="0" pageNumber="99">I</emphasis>
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internal raphe fissures terminate in helictoglossae at the apices (arrowhead)
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<emphasis id="AA5B97B2059B1516185020E2C5408A4A" bold="true" pageId="0" pageNumber="99">J</emphasis>
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external view of the whole valve showing the terminal fissures curving into opposite directions
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<emphasis id="A46D1DB0AC805983258B64756DC819D5" bold="true" pageId="0" pageNumber="99">K</emphasis>
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||
external central area of valve showing straight, simple raphe fissures (arrowhead)
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<emphasis id="546F95D245DE7A4C958E0F3046AA2D23" bold="true" pageId="0" pageNumber="99">L</emphasis>
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terminal fissures (arrowhead). Scale bars: 10
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<normalizedToken id="DCE62150FB19E275042289726933A832" originalValue="μm">μm</normalizedToken>
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||
(
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<emphasis id="C502BAE349B0BFCF75A97963C916EE42" bold="true" pageId="0" pageNumber="99">A-F</emphasis>
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||
); 5
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||
<normalizedToken id="F48F57595BE1BC076CE3C08CEA12A247" originalValue="μm">μm</normalizedToken>
|
||
(
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||
<emphasis id="95B08818A6C6E5055B05CDE021A3E08C" bold="true" pageId="0" pageNumber="99">G</emphasis>
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||
,
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||
<emphasis id="6FFFDA0C8A2538237FFC5260AF9E7CCD" bold="true" pageId="0" pageNumber="99">J</emphasis>
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||
); 1
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<normalizedToken id="6126BE88DFB93E34143EAFD22CA7694B" originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis id="DB3A959A0EB7405104A62CF6E5EAF2DC" bold="true" pageId="0" pageNumber="99">H</emphasis>
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||
,
|
||
<emphasis id="A2BCDD96F71B2E48B9007DBBB2E9A1A3" bold="true" pageId="0" pageNumber="99">I</emphasis>
|
||
,
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||
<emphasis id="F1BF3EA634B385EB7A58A6150A2342C4" bold="true" pageId="0" pageNumber="99">K</emphasis>
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||
,
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<emphasis id="DF029EBE88475F32F8EDA13B07AE7B46" bold="true" pageId="0" pageNumber="99">L</emphasis>
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||
).
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</paragraph>
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</caption>
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<paragraph id="61C92C00F9DF54ECADE763D16A8D7F08" pageId="0" pageNumber="99">
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In SEM, internally, raphe fissures terminate in helictoglossae at apices, orientated in opposite direction to one another (Fig.
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<figureCitation id="91057D053E9FB83AE50E52C9D07AB8E1" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1G, I</figureCitation>
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, arrowhead). Internal central fissures straight, slightly expanded (Fig.
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<figureCitation id="9CAD92CC64D4E7C900F503E332DEF5AD" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1H</figureCitation>
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, arrowhead). Central nodule is raised and flanked by two central bars (Fig.
|
||
<figureCitation id="289172D04776BA218B7DA474A1EE64A1" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1H</figureCitation>
|
||
). External central raphe fissures straight, simple (Fig.
|
||
<figureCitation id="8F349E165BCAC29937D4954B2023DBB8" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1K</figureCitation>
|
||
, arrowhead). Terminal fissures bent to opposite side of valve, not extending onto valve mantle (Fig.
|
||
<figureCitation id="1A6FC01B64F4D8CBB66304E4CD8C330D" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1L</figureCitation>
|
||
, arrowhead). Areolae loculate with external opening slits and internal poroids (Fig.
|
||
<figureCitation id="6EF68F017A97B28F1E55934647B7366A" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1G, J</figureCitation>
|
||
).
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="3EF7FC5F98DA90CFBB995525401DA06C" pageId="0" pageNumber="99" type="holotype">
|
||
<paragraph id="D76D8498A178F7CF4391DE39D05BCF02" pageId="0" pageNumber="99">Holotype.</paragraph>
|
||
<paragraph id="42E1A0944E9D2BB8CDD054E000A86523" pageId="0" pageNumber="99">
|
||
MBMCAS286904, an example is illustrated in Fig.
|
||
<figureCitation id="8BB61C4519C1B26F71A0F3744A4CDA00" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1A, E, F</figureCitation>
|
||
. This slide was deposited in the Marine Biological Museum, Chinese Academy of Sciences, Qingdao, China.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="1F3B5A5981D46C1A1422C5F39CFE0A1C" pageId="0" pageNumber="99" type="isotype">
|
||
<paragraph id="720D5BEC6D4CEBD1C64AE4DD93803AF4" pageId="0" pageNumber="99">Isotype.</paragraph>
|
||
<paragraph id="CDA3B93F9DFE9A620D14E86C10D8A638" pageId="0" pageNumber="99">
|
||
MBMCAS286905, an example is illustrated in Fig.
|
||
<figureCitation id="9DF696A64DCBD52F91A1D18174F08DC4" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. A-F LM photographs of P. pacificum A-D cleaned frustules showing parallel transverse striae under LM E valve center showing a roundish central area (arrowhead) F funnel-shaped terminal area in apical position G-L SEM photographs of P. pacificum G internal view of the whole valve H internal valve central area showing straight, slight expanded raphe fissures (arrowhead) and nodule bordered by two arched bars I internal raphe fissures terminate in helictoglossae at the apices (arrowhead) J external view of the whole valve showing the terminal fissures curving into opposite directions K external central area of valve showing straight, simple raphe fissures (arrowhead) L terminal fissures (arrowhead). Scale bars: 10 μm (A-F); 5 μm (G, J); 1 μm (H, I, K, L)." figureDoi="10.3897/phytokeys.227.103890.figure1" httpUri="https://binary.pensoft.net/fig/858193" pageId="0" pageNumber="99">1C</figureCitation>
|
||
. This slide was deposited in the Marine Biological Museum, Chinese Academy of Sciences, Qingdao, China.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="ADA8073D1494AB6F021A2FF0F48F0DB9" pageId="0" pageNumber="99" type="type locality">
|
||
<paragraph id="7E1D3D2628E8147C5F9EE00665F15E84" pageId="0" pageNumber="99">Type locality.</paragraph>
|
||
<paragraph id="50665D41E67879E8908053780060FCB4" pageId="0" pageNumber="99">
|
||
<geoCoordinate id="FF181075768419FD4A01ED7AA90C0B7E" degrees="7" direction="north" minutes="0.26" orientation="latitude" precision="9" value="7.0043335">7°0.26'N</geoCoordinate>
|
||
,
|
||
<geoCoordinate id="C0911F71E648B1CB22C16BDB9F1EA549" degrees="141" direction="east" minutes="59.63" orientation="longitude" precision="9" value="141.99384">141°59.63'E</geoCoordinate>
|
||
, the upper 200 m water column in the tropical Western Pacific Ocean.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="9F5744E59DB34B5F8CCF188031490590" pageId="0" pageNumber="99" type="etymology">
|
||
<paragraph id="D2DE40058C07AD1F2D09D73602987786" pageId="0" pageNumber="99">Etymology.</paragraph>
|
||
<paragraph id="9350785374851A32C5A67A931C784C7A" pageId="0" pageNumber="99">Named after the Pacific Ocean where the species was discovered.</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="E15EDC3147F1C936C372EFAD3C2AFE5F" pageId="0" pageNumber="99" type="distribution">
|
||
<paragraph id="F0BC913EA6D05D2F6B0110393E5258E9" pageId="0" pageNumber="99">Distribution and ecology.</paragraph>
|
||
<paragraph id="FD9505FC9856CE499A4D9BC0826A4598" pageId="0" pageNumber="99">
|
||
<taxonomicName id="2DC2953F539DF3CFF6215CFBA9C56172" authorityName="Du & Li & Xu" authorityYear="2023" class="Bacillariophyceae" family="Pleurosigmataceae" genus="Pleurosigma" kingdom="Plantae" lsidName="Pleurosigma pacificum" order="Naviculales" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="pacificum">
|
||
<emphasis id="BE04B80A2ACF46041EFFCE5052078621" italics="true" pageId="0" pageNumber="99">Pleurosigma pacificum</emphasis>
|
||
</taxonomicName>
|
||
is a planktonic species known only from the type locality. The water temperature was 28.5 °C and salinity about 33.4 during sampling.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="D1AE7E16F44A3415E49ADFA9675C5723" pageId="0" pageNumber="99" type="gene sequences">
|
||
<paragraph id="9BB29F3400FEE590721826CA4A70090D" pageId="0" pageNumber="99">Gene sequences.</paragraph>
|
||
<paragraph id="AB6F75C6732C2773741F4FC169C9EF49" pageId="0" pageNumber="99">
|
||
These sequences were deposited in the GenBank (SSU rDNA OQ437519, LSU rDNA OQ549922,
|
||
<emphasis id="C5C472743F11A49C5847A9168560C119" italics="true" pageId="0" pageNumber="99">rbc</emphasis>
|
||
L OQ473490 and
|
||
<emphasis id="F00A1B804B51691CA1C2CD5D9AE8DB11" italics="true" pageId="0" pageNumber="99">psb</emphasis>
|
||
C OQ437519).
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="A9F95835D660105F6A646D212B54318F" pageId="0" pageNumber="99" type="phycobank registration">
|
||
<paragraph id="5339CF1D38274C1A4E4C04A84C08E3FC" pageId="0" pageNumber="99">PhycoBank registration.</paragraph>
|
||
<paragraph id="6DF32D5B8F3F218A319D65D971DC5F4B" pageId="0" pageNumber="99">http://phycobank.org/103761.</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="EF73D4F455BD0AE3AFA90A5426F9CDC2" pageId="0" pageNumber="99" type="molecular phylogenetic analyses">
|
||
<paragraph id="88A158742A8616EBD83F9E34DEBC7823" pageId="0" pageNumber="99">Molecular phylogenetic analyses</paragraph>
|
||
<paragraph id="9FC7E1F958C981288FCB4FAA2DAE1BEE" pageId="0" pageNumber="99">
|
||
The BLASTn search showed that the SSU rDNA sequence of
|
||
<taxonomicName id="BBE62FFD1601245A5D12FD022A74BE87" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. pacificum" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="pacificum">
|
||
<emphasis id="3815092C1ED49D2C47E1D925B628C0F7" italics="true" pageId="0" pageNumber="99">P. pacificum</emphasis>
|
||
</taxonomicName>
|
||
shares 97.65% identity with an uncultured marine eukaryote (KC771201). The
|
||
<emphasis id="455717E722007AA35B6BA96988849DEE" italics="true" pageId="0" pageNumber="99">rbc</emphasis>
|
||
L gene sequence of
|
||
<taxonomicName id="563E7A32EAF239F29A2EEFDF99341DBC" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. pacificum" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="pacificum">
|
||
<emphasis id="0513094A31415AA832FCF7C9768BC45F" italics="true" pageId="0" pageNumber="99">P. pacificum</emphasis>
|
||
</taxonomicName>
|
||
shares 92.54% identity with
|
||
<taxonomicName id="3163A5685E574F527EDAE57CFF8FCA03" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. intermedium" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="intermedium">
|
||
<emphasis id="B964F3EDCA70933ED64A0A59C17F3324" italics="true" pageId="0" pageNumber="99">P. intermedium</emphasis>
|
||
</taxonomicName>
|
||
(NC_066077). The ML, BI and MP phylogenetic tree based on the concatenated SSU rDNA and
|
||
<emphasis id="CE0561A1F2680AE806702C13BE51D1C2" italics="true" pageId="0" pageNumber="99">rbc</emphasis>
|
||
L gene showed that
|
||
<taxonomicName id="1F15101DE94417C4CE32439A78CC9EE1" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. pacificum" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="pacificum">
|
||
<emphasis id="7E44705F78C6B593F307EC9B8A6AD1F5" italics="true" pageId="0" pageNumber="99">P. pacificum</emphasis>
|
||
</taxonomicName>
|
||
belongs to the clade of the genus
|
||
<taxonomicName id="BA49414351831630C8C42B56222CCEE2" authorityName="W.Smith" authorityYear="1852" class="Bacillariophyceae" family="Pleurosigmataceae" genus="Pleurosigma" kingdom="Plantae" lsidName="Pleurosigma" order="Naviculales" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="genus">
|
||
<emphasis id="D20F4024558D98FAE199D69C7E6929BE" italics="true" pageId="0" pageNumber="99">Pleurosigma</emphasis>
|
||
</taxonomicName>
|
||
with high support (IQ-TREE ultrafast bootstrap value = 99, Mrbayes posterior probability = 1.00, MP standard boostrap value = 77 and MP jacknife value = 85). The
|
||
<taxonomicName id="5F55E0E7F5E0553A2209A053829FB131" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. pacificum" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="pacificum">
|
||
<emphasis id="D9FD56F18B3700E352D76E4A5AD0C5AC" italics="true" pageId="0" pageNumber="99">P. pacificum</emphasis>
|
||
</taxonomicName>
|
||
is basal to all other species of
|
||
<taxonomicName id="BC536F7F793C4FC3194E31AAC487213A" authorityName="W.Smith" authorityYear="1852" class="Bacillariophyceae" family="Pleurosigmataceae" genus="Pleurosigma" kingdom="Plantae" lsidName="Pleurosigma" order="Naviculales" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="genus">
|
||
<emphasis id="1BDB8396DA2FAB38FCC45F73ABE2DDDF" italics="true" pageId="0" pageNumber="99">Pleurosigma</emphasis>
|
||
</taxonomicName>
|
||
, and branched earlier than the two slightly sigmoid species,
|
||
<taxonomicName id="FE2D4CE1676531CE1735C4DE84139F1C" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. planctonicum" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="planctonicum">
|
||
<emphasis id="3BDFBB82016C34E0EB2C7C594260C310" italics="true" pageId="0" pageNumber="99">P. planctonicum</emphasis>
|
||
</taxonomicName>
|
||
and
|
||
<taxonomicName id="D78A54ABD9BA534AC95EC3203920DF8A" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="P. intermedium" pageId="0" pageNumber="99" phylum="Bacillariophyta" rank="species" species="intermedium">
|
||
<emphasis id="4C175F98D512E3040D8C92465B1444AF" italics="true" pageId="0" pageNumber="99">P. intermedium</emphasis>
|
||
</taxonomicName>
|
||
(Fig.
|
||
<figureCitation id="100577C91D653149342CDC1705EFB28C" captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Maximum likelihood (ML) and Bayesian inference (BI) phylogenetic trees based on the concatenated SSU rDNA and rbc L sequences. The values on each node indicate ML bootstrap and Bayesian posterior probabilities (%), respectively. Only bootstrap values over 50 % are shown on the tree. Black dot indicate ML / BI = 100 / 1.00. The asterisk indicates the topological incongruence between ML and BI trees." figureDoi="10.3897/phytokeys.227.103890.figure2" httpUri="https://binary.pensoft.net/fig/858194" pageId="0" pageNumber="99">2</figureCitation>
|
||
, Suppl. material 2).
|
||
</paragraph>
|
||
<caption id="972A2BFFC198025463835D5C22E8ED0E" doi="10.3897/phytokeys.227.103890.figure2" httpUri="https://binary.pensoft.net/fig/858194" pageId="0" pageNumber="99" start="Figure 2" startId="F2">
|
||
<paragraph id="06888A049F97F517AE00B1CD74EAC28D" pageId="0" pageNumber="99">
|
||
<emphasis id="577E872FAFAA6A7DA38B6915AF9D46AE" bold="true" pageId="0" pageNumber="99">Figure 2.</emphasis>
|
||
Maximum likelihood (ML) and Bayesian inference (BI) phylogenetic trees based on the concatenated SSU rDNA and
|
||
<emphasis id="90F9E0FF23C046EEFE241395D95FCAC3" italics="true" pageId="0" pageNumber="99">rbc</emphasis>
|
||
L sequences. The values on each node indicate ML bootstrap and Bayesian posterior probabilities (%), respectively. Only bootstrap values over 50% are shown on the tree. Black dot indicate ML/BI = 100/1.00. The asterisk indicates the topological incongruence between ML and BI trees.
|
||
</paragraph>
|
||
</caption>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |