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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.101.115370" ID-Pensoft-Pub="1314-4049-101-1" ID-Pensoft-UUID="FBF8EACC1A7B50CA918E4C7D2FB08E8E" ModsDocID="1314-4049-101-1" checkinTime="1704593452343" checkinUser="pensoft" docAuthor="He, Jiao, Li, De-Wei, Cui, Wen-Li &amp; Huang, Lin" docDate="2024" docId="329053323E805CE9A233A601C7A1D8B2" docLanguage="en" docName="MycoKeys 101: 1-44" docOrigin="MycoKeys 101" docPubDate="2024-01-05" docSource="http://dx.doi.org/10.3897/mycokeys.101.115370" docTitle="Alternaria xinyangensis Lin Huang, Jiao He &amp; D. W. Li 2024, sp. nov." docType="treatment" docVersion="2" id="FBF8EACC1A7B50CA918E4C7D2FB08E8E" lastPageNumber="1" masterDocId="FBF8EACC1A7B50CA918E4C7D2FB08E8E" masterDocTitle="Seven new species of Alternaria (Pleosporales, Pleosporaceae) associated with Chinese fir, based on morphological and molecular evidence" masterLastPageNumber="44" masterPageNumber="1" pageNumber="1" updateTime="1704593756116" updateUser="ExternalLinkService">
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<mods:title id="E06A076A57C9FC1083874D8BC9A7BAFA">Seven new species of Alternaria (Pleosporales, Pleosporaceae) associated with Chinese fir, based on morphological and molecular evidence</mods:title>
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<mods:namePart id="4A66BE495D4A3A890E38C2AC4D01352A">He, Jiao</mods:namePart>
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<mods:affiliation id="C4A115203B1FA733CF38BDC49A1F63A6">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
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<mods:namePart id="E9A64EA8CA69321068812073298EC514">Li, De-Wei</mods:namePart>
<mods:nameIdentifier id="0AE2DDBCF6CE8914C5C883305492ABEB" type="ORCID">https://orcid.org/0000-0002-2788-7938</mods:nameIdentifier>
<mods:affiliation id="75131C92F7EAD35D9B3EE88A6D07638D">The Connecticut Agricultural Experiment Station Valley Laboratory, Windsor, CT 06095, USA</mods:affiliation>
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<mods:namePart id="F6DAD64A291296F67DD537129D467E05">Cui, Wen-Li</mods:namePart>
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<mods:affiliation id="07A21E1DE198CFFA3198B229261FEFBD">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
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<mods:namePart id="D59D4B0EF437580D9BDBB3789739FCA0">Huang, Lin</mods:namePart>
<mods:nameIdentifier id="74A884FE0E5EBEF607DA0D261EDE23D7" type="ORCID">https://orcid.org/0000-0001-7536-0914</mods:nameIdentifier>
<mods:affiliation id="CA54DC4C5AAA055C1985A3244472DD8A">Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu 210037, China</mods:affiliation>
<mods:nameIdentifier id="A05CA1002E1AADD61B9387064BAFF371" type="email">lhuang@njfu.edu.cn</mods:nameIdentifier>
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<mods:date id="BB1D8E1239D08E546A317911BDA5B2FE">2024</mods:date>
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<mods:number id="8C8F2C78A41BE79C9B89F94AD2F206C3">2024-01-05</mods:number>
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<treatment id="329053323E805CE9A233A601C7A1D8B2" LSID="urn:lsid:plazi:treatment:329053323E805CE9A233A601C7A1D8B2" httpUri="http://treatment.plazi.org/id/329053323E805CE9A233A601C7A1D8B2" lastPageNumber="1" pageId="0" pageNumber="1">
<subSubSection id="C32CC1F83F3CD26CF8C91B914EDC547A" pageId="0" pageNumber="1" type="nomenclature">
<paragraph id="BE2443D9F416206E6F8224879DEB9984" pageId="0" pageNumber="1">
<taxonomicName id="680826F97C234C786A4E2F4F9A5C00A4" LSID="32905332-3E80-5CE9-A233-A601C7A1D8B2" authority="Lin Huang, Jiao He &amp; D. W. Li" authorityName="Lin Huang, Jiao He &amp; D. W. Li" authorityYear="2024" class="Dothideomycetes" family="Pleosporaceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria xinyangensis" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="xinyangensis" status="sp. nov.">Alternaria xinyangensis Lin Huang, Jiao He &amp; D.W. Li</taxonomicName>
<taxonomicNameLabel id="6F4C967A3C5377343A485651DF43E9D6" pageId="0" pageNumber="1">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection id="F49D011DD392061BA6006E47AF14E4DA" pageId="0" pageNumber="1" type="description">
<paragraph id="702DD7D297892599D9839CD67C91536F" pageId="0" pageNumber="1">
<figureCitation id="DED4473CDE216FBA743A6AA1C9B6B54F" captionStart="Figure 9" captionStartId="F9" captionText="Figure 9. Alternaria xinyangensis (ZLS 1) A colony on PCA after 6 days at 25 ° C in the dark B, C sporulation patterns D conidiophores and conidiogenouse cells E conidium. Scale bars: 50 μm (B, C); 10 μm (D, E)." figureDoi="10.3897/mycokeys.101.115370.figure9" httpUri="https://binary.pensoft.net/fig/962741" pageId="0" pageNumber="1">Fig. 9</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="075D251C6E9D33EC1EE7E4CBAFAD64AB" pageId="0" pageNumber="1" type="holotype">
<paragraph id="C348F26BEDB1E6040BBE95E466206E30" pageId="0" pageNumber="1">Holotype.</paragraph>
<paragraph id="F116D4AD26F9E49F468EFCA16E34D638" pageId="0" pageNumber="1">
China, Henan Province, Xinyang City, Zhenlei Mountain,
<geoCoordinate id="99C1667D5D9DDE7BB6E87C4E35F85E2A" degrees="32" direction="north" minutes="04" orientation="latitude" precision="15" seconds="51" value="32.080833">32°04'51&quot;N</geoCoordinate>
,
<geoCoordinate id="6D632B97A922D19F23544569C0AED4A7" degrees="114" direction="east" minutes="07" orientation="longitude" precision="15" seconds="23" value="114.123055">114°07'23&quot;E</geoCoordinate>
, isolated from leaf spots of
<taxonomicName id="882FC015CC25A3646513F81416147B12" class="Mucoromycetes" family="Choanephoraceae" genus="Cunninghamia" kingdom="Fungi" lsidName="Cunninghamia lanceolata" order="Mucorales" pageId="0" pageNumber="1" phylum="Zygomycota" rank="species" species="lanceolata">
<emphasis id="91CFCC903580F58D4D8E007ADE6C4B4A" italics="true" pageId="0" pageNumber="1">Cunninghamia lanceolata</emphasis>
</taxonomicName>
, May 2017, Wen-Li Cui, (holotype: CFCC 59352). Holotype specimen is a living specimen being maintained via lyophilisation at the China Forestry Culture Collection Center (CFCC). Ex-type (ZLS1) is maintained at the Forest Pathology Laboratory, Nanjing Forestry University.
</paragraph>
</subSubSection>
<subSubSection id="D63EAE1B145AD1B34E30FB61BF692AAD" pageId="0" pageNumber="1" type="etymology">
<paragraph id="FAD4ABDDFB5DCA8C71F98D15F975B7FD" pageId="0" pageNumber="1">Etymology.</paragraph>
<paragraph id="C72D0DA892652172E514A55F989237AB" pageId="0" pageNumber="1">Epithet is after Xinyang City where the type specimen was collected.</paragraph>
</subSubSection>
<subSubSection id="BF67B479C57FF15E36364D0299DAE629" pageId="0" pageNumber="1" type="distribution">
<paragraph id="406C41C8DB936C9A6A53EBB3092E9B33" pageId="0" pageNumber="1">Host/distribution.</paragraph>
<paragraph id="D2B9491A554A29F828F830250563E0EE" pageId="0" pageNumber="1">
From
<taxonomicName id="8A0F1BE679493E008D9ADD39DC698627" authorityName="González-Sponga" authorityYear="1987" lsidName="C. lanceolata" pageId="0" pageNumber="1" rank="species" species="lanceolata">
<emphasis id="35BF1F0A635DDAC0CA13EFF696CF792F" italics="true" pageId="0" pageNumber="1">C. lanceolata</emphasis>
</taxonomicName>
in Zhenlei Mountain, Xinyang City, Henan Province, China.
</paragraph>
</subSubSection>
<subSubSection id="864CEDC09EA5992B8C53D85D116FA773" pageId="0" pageNumber="1" type="description">
<paragraph id="64C2062C475C15CA2FE9F334C4205E16" pageId="0" pageNumber="1">Description.</paragraph>
<paragraph id="720C64530DADBFD76085FA305E3AB5F0" pageId="0" pageNumber="1">
Mycelium
<normalizedToken id="B2694E4C53666982BF5BAF752358914E" originalValue="superficial">superficial</normalizedToken>
on the PCA, composed of septate, branched, smooth, thin-walled, white to light brown hyphae. Conidiophores macronematous, mononematous, produced laterally or terminally on the hyphae, cylindrical, erect or ascending, simple or branched, geniculate, pale brown to dark brown, smooth, 1-7 septate, (9.4-)15.3-54.9(-80.4)
<normalizedToken id="6EB1AFEA54DC1A19DC704D9000A1A634" originalValue="×">x</normalizedToken>
(2.9-)3.7-4.8(-5.2)
<normalizedToken id="B895035893C6224972985A4168C318C5" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="4EB09D1844929155AA2CC8E205CFDDE1" originalValue="±">+/-</normalizedToken>
SD = 35.1
<normalizedToken id="3104DE806C231212A90763768EF6AAC5" originalValue="±">+/-</normalizedToken>
19.8
<normalizedToken id="7574C10123DC7B8C6A1037939B2B207F" originalValue="×">x</normalizedToken>
4.2
<normalizedToken id="ADCCC9FFE445756BAC3E2CD0278B682C" originalValue="±">+/-</normalizedToken>
0.6
<normalizedToken id="B1D1D47E41B08E1931C46DBE5FF752A9" originalValue="μm">μm</normalizedToken>
, n = 40). Conidiogenous cells apical or subapical, cylindrical, brown, smooth, (3.9-)5.3-9.6(-12.9)
<normalizedToken id="AE22AF4FDE5733CB3A44B19BD4991569" originalValue="×">x</normalizedToken>
(2.4-)3.3-4.9(-5.5)
<normalizedToken id="EEDF078C75E13CCDDD2342452B416D8B" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="FB6A7885CE480ECCAB71CF1087CF4970" originalValue="±">+/-</normalizedToken>
SD = 7.5
<normalizedToken id="3EB4E3730A81FFFBCAEFBA9C5B1D185A" originalValue="±">+/-</normalizedToken>
2.2
<normalizedToken id="A5E66288537440488E3296C0041386EA" originalValue="×">x</normalizedToken>
4.1
<normalizedToken id="555244410C1073FB67E5EF20B2E8BE74" originalValue="±">+/-</normalizedToken>
0.8
<normalizedToken id="D2107D0DCD962C7BF9AF58184AAB348D" originalValue="μm">μm</normalizedToken>
, n = 39), mono- or polytretic, with conspicuous scars after conidia have seceded. Each conidiogenous locus bears a primary chain of 2-7 conidia; each primary chain usually has 1-3 branching chains of 1-3 conidia. Newly-developed conidia subhyaline or pale greyish, ellipsoidal or subacute, thin-walled, 1-3 septate, with few or no protuberance. Mature conidia brown to dark chocolate-brown, spheroidal or ellipsoid to long-ellipsoid, with 1-6 transverse septa and 1-5 longitudinal or oblique septa, (13.8-)19.9-31.8(-37.6)
<normalizedToken id="C4A5E69A39689717E938F4FE9197B73B" originalValue="×">x</normalizedToken>
(6.9-)8.6-12.9(-17.5)
<normalizedToken id="C792757845EF0D0618564DF3DCE3A9B3" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="8AB846D60C6EAAF9D7695AB888F8549C" originalValue="±">+/-</normalizedToken>
SD = 25.9
<normalizedToken id="F2E4098245C5B9FCAB4D1D4AFB3FC41E" originalValue="±">+/-</normalizedToken>
6.0
<normalizedToken id="C2FDCE7D948E7E814A9004389F09ED57" originalValue="×">x</normalizedToken>
10.7
<normalizedToken id="CA8FBEC17312F5CC87BF91ED4B29BBF2" originalValue="±">+/-</normalizedToken>
2.1
<normalizedToken id="8BC88D121BF02518F8F87374C24B37EF" originalValue="μm">μm</normalizedToken>
, n = 37) in size. Secondary conidia commonly produced by means of a short apical or lateral secondary conidiophore, but rarely by conidia through an inconspicuous apical conidiogenous locus. In addition, false beaks (secondary conidiophores), unbranched, short, blunted, pale brown, (3.0-)5.3-16.0(-24.4)
<normalizedToken id="C912BBF50C88E1344F1846568C292AE5" originalValue="×">x</normalizedToken>
(2.4-)2.8-4.1(-5.1)
<normalizedToken id="70E2F92BD07A60707931CAAE3A178B4F" originalValue="μm">μm</normalizedToken>
, (mean
<normalizedToken id="EC6CE1389DCD7C16E320238F459B7B9F" originalValue="±">+/-</normalizedToken>
SD = 10.6
<normalizedToken id="EC4876ECE99041BF587515441BF9DA2F" originalValue="±">+/-</normalizedToken>
5.4
<normalizedToken id="6C4B7BF38502585056F7E62B95F03653" originalValue="×">x</normalizedToken>
3.4
<normalizedToken id="CE0BA0CA039CC767E67FA4100F137E1C" originalValue="±">+/-</normalizedToken>
0.7
<normalizedToken id="97DF33B9228AE37E5DFA15568C70DF89" originalValue="μm">μm</normalizedToken>
, n = 31). Conidial beakless mostly with a conical cell at the apex. Chlamydospores not observed.
</paragraph>
<caption id="B2324B0D0696B1F8E603388E59C54301" doi="10.3897/mycokeys.101.115370.figure9" httpUri="https://binary.pensoft.net/fig/962741" pageId="0" pageNumber="1" start="Figure 9" startId="F9">
<paragraph id="56D3822AC63B19684B39246B3A1DF688" pageId="0" pageNumber="1">
<emphasis id="19F6BCAC0014028901BB23486D95D28B" bold="true" pageId="0" pageNumber="1">Figure 9.</emphasis>
<taxonomicName id="78928812E4C67080F9D6048F4BAF0CFB" authorityName="Lin Huang, Jiao He &amp; D. W. Li" authorityYear="2024" class="Dothideomycetes" family="Moraceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria xinyangensis" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="xinyangensis">
<emphasis id="6B4A42C180045B61FE8EB340A6B26A11" italics="true" pageId="0" pageNumber="1">Alternaria xinyangensis</emphasis>
</taxonomicName>
(ZLS1)
<emphasis id="16634AA1AFCA3168ECD53EF956B7F9BA" bold="true" pageId="0" pageNumber="1">A</emphasis>
colony on PCA after 6 days at 25 °C in the dark
<emphasis id="1FAB3E4E23BC19D9DA82B28E8442581D" bold="true" pageId="0" pageNumber="1">B, C</emphasis>
sporulation patterns
<emphasis id="E5DDB213AC4B8DF831ADDD439A7130B7" bold="true" pageId="0" pageNumber="1">D</emphasis>
conidiophores and conidiogenouse cells
<emphasis id="5DA91F34B4AA76857A3F4BF43C6BD566" bold="true" pageId="0" pageNumber="1">E</emphasis>
conidium. Scale bars: 50
<normalizedToken id="9C518FA6AA5B7C17D7BD8A62E67E2741" originalValue="μm">μm</normalizedToken>
(
<emphasis id="B2ECDC16267AA0E3AC19186A821C7A33" bold="true" pageId="0" pageNumber="1">B, C</emphasis>
);10
<normalizedToken id="0C4C0CE1CC17ECB2798A18F6B67B5E17" originalValue="μm">μm</normalizedToken>
(
<emphasis id="A932FBB964A1B1853DDEC7F55E2624F7" bold="true" pageId="0" pageNumber="1">D, E</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="7580C238726A55E1385F09F4D19A865D" pageId="0" pageNumber="1" type="description">
<paragraph id="E490ED6681059B84D3FB2CFF76AC12A0" pageId="0" pageNumber="1">Culture characteristics.</paragraph>
<paragraph id="072019E28DB19D37AF997EAE1FD96F81" pageId="0" pageNumber="1">Colonies on PCA incubated at 25 °C in the dark growing at 7.2 mm/d; aerial hyphae cottony, olive green, with white margins; reverse centre black to greyish; sporulation abundant; diffusible pigment absent.</paragraph>
</subSubSection>
<subSubSection id="8BF77E3AF3262A5B43298F30C528B44C" pageId="0" pageNumber="1" type="materials_examined">
<paragraph id="65BB0F00D10DA7EBC9B97F3BBD8307E0" pageId="0" pageNumber="1">Additional materials examined.</paragraph>
<paragraph id="58CADFDCF4A02DD0819014014E4FE01D" pageId="0" pageNumber="1">
<materialsCitation id="84FCF86107F7ADB6C2744FCF0A746948" collectingDate="2017-05" country="China" latitude="32.080833" location="Zhenlei Mountain" longLatPrecision="20" longitude="114.123055" municipality="Xinyang City" specimenCount="1" stateProvince="Henan Province">
<collectingCountry id="B47676A216EF63CFB7F5B13987926BB2" name="China">China</collectingCountry>
,
<collectingRegion id="11E82DDC96007C766960772C24DCE5FE" country="China" name="Henan">Henan Province</collectingRegion>
,
<collectingMunicipality id="BFAEB685F4F799CA3885A7513AF5E4FA">Xinyang City</collectingMunicipality>
,
<location id="C7C4F4919D1BBF2C54322B38E390B230" LSID="urn:lsid:plazi:treatment:329053323E805CE9A233A601C7A1D8B2:C7C4F4919D1BBF2C54322B38E390B230" country="China" latitude="32.080833" longLatPrecision="20" longitude="114.123055" municipality="Xinyang City" name="Zhenlei Mountain" stateProvince="Henan Province">Zhenlei Mountain</location>
,
<geoCoordinate id="8323B1B01BDF031B3B6E81D5CBD4702F" degrees="32" direction="north" minutes="04" orientation="latitude" precision="15" seconds="51" value="32.080833">32°04'51&quot;N</geoCoordinate>
,
<geoCoordinate id="EC1D19E577FA7B764ACCAA635F18DD7A" degrees="114" direction="east" minutes="07" orientation="longitude" precision="15" seconds="23" value="114.123055">114°07'23&quot;E</geoCoordinate>
, isolated from leaf spots of
<taxonomicName id="A59BB12E9FA2A6849D8C4CA787D85EEF" class="Mucoromycetes" family="Choanephoraceae" genus="Cunninghamia" kingdom="Fungi" lsidName="Cunninghamia lanceolata" order="Mucorales" pageId="0" pageNumber="1" phylum="Zygomycota" rank="species" species="lanceolata">
<emphasis id="828899A4D3FA095E6063860E407C82A3" italics="true" pageId="0" pageNumber="1">Cunninghamia lanceolata</emphasis>
</taxonomicName>
,
<collectingDate id="8797665E7EE2F92244870B93CB3E2AA4" value="2017-05">May 2017</collectingDate>
, Wen-Li Cui, ZLS1-1, ZLS1-2, ZLS1-3, ZLS1-4
</materialsCitation>
;
<materialsCitation id="989F8F728AED0C0DCC9FED78E849BB89" collectingDate="2017-05" country="China" latitude="32.13889" location="Xinyang University" longLatPrecision="20" longitude="114.034996" municipality="Xinyang City" specimenCount="1" stateProvince="Henan Province">
<collectingCountry id="C422132F31B7ECB9ED4020D1944FD975" name="China">China</collectingCountry>
,
<collectingRegion id="30AA070CDE0D698B782B28F0E491F689" country="China" name="Henan">Henan Province</collectingRegion>
,
<collectingMunicipality id="D400034F5A0775F9C8DBD46DA66FF7C6">Xinyang City</collectingMunicipality>
,
<location id="4CD1BF3031CBF3861CC2804D6E6F19AE" LSID="urn:lsid:plazi:treatment:329053323E805CE9A233A601C7A1D8B2:4CD1BF3031CBF3861CC2804D6E6F19AE" country="China" latitude="32.13889" longLatPrecision="20" longitude="114.034996" municipality="Xinyang City" name="Xinyang University" stateProvince="Henan Province">Xinyang University</location>
,
<geoCoordinate id="68A467469D3CDCBB53B832A5599A8C02" degrees="32" direction="north" minutes="08" orientation="latitude" precision="15" seconds="20" value="32.13889">32°08'20&quot;N</geoCoordinate>
,
<geoCoordinate id="6C8A73F448FA6008B74A04F1B68AEB46" degrees="114" direction="east" minutes="02" orientation="longitude" precision="15" seconds="06" value="114.034996">114°02'06&quot;E</geoCoordinate>
, isolated from leaf spots of
<taxonomicName id="70D0C0BC9CEA71C3A3137840D5CA0E07" authorityName="González-Sponga" authorityYear="1987" lsidName="C. lanceolata" pageId="0" pageNumber="1" rank="species" species="lanceolata">
<emphasis id="851471870E052F5277C008428D87CE53" italics="true" pageId="0" pageNumber="1">C. lanceolata</emphasis>
</taxonomicName>
,
<collectingDate id="AB46F399CB78D558E2CDABC698C4A7D9" value="2017-05">May 2017</collectingDate>
, Wen-Li Cui, XYXY06, XYXY8-2, XYXY15, XYXY15-1, XYXY15-2, XYXY15-3, XYXY15-4, XYXY16
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="6F4762EB9D9313DD04B1B04F96F40B35" pageId="0" pageNumber="1" type="notes">
<paragraph id="83FE98FE3E5665396197AFC946A49091" pageId="0" pageNumber="1">Notes.</paragraph>
<paragraph id="B80C2544C0589382557C04BBC290A1DA" pageId="0" pageNumber="1">
The isolates of
<taxonomicName id="EDC0916F0B37EF3E8E90F2061127836D" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="0CDE3402BD5D59E95FBAB4F19CAD1A47" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
were phylogenetically close to
<taxonomicName id="74BB4675AA8218C10BCA5547C7FE90C9" lsidName="A. dongshanqiaoensis" pageId="0" pageNumber="1" rank="species" species="dongshanqiaoensis">
<emphasis id="9D512F3408F0E6BE54604656C9E41E99" italics="true" pageId="0" pageNumber="1">A. dongshanqiaoensis</emphasis>
</taxonomicName>
(in this study, DSQ2-2),
<taxonomicName id="786361CCB1BA33950666CD5DA1651955" lsidName="A. citri" pageId="0" pageNumber="1" rank="species" species="citri">
<emphasis id="D2C414D381E950EEF6E333880CDC3869" italics="true" pageId="0" pageNumber="1">A. citri</emphasis>
</taxonomicName>
(ex-epitype, CBS 107.27),
<taxonomicName id="4B1A47C3F6EEDB74DC682EAFD9811CAB" lsidName="A. cinerariae" pageId="0" pageNumber="1" rank="species" species="cinerariae">
<emphasis id="68662C06B22DC2396891FA8A3A2A0BA9" italics="true" pageId="0" pageNumber="1">A. cinerariae</emphasis>
</taxonomicName>
(ex-epitype, CBS 612.72) and
<taxonomicName id="44B2E4469E03EE4FD93309813FE4AE37" lsidName="A. kikuchiana" pageId="0" pageNumber="1" rank="species" species="kikuchiana">
<emphasis id="FF3165D3E05B427416676A10836ADC52" italics="true" pageId="0" pageNumber="1">A. kikuchiana</emphasis>
</taxonomicName>
(ex-type, CBS 107.53) (Fig.
<figureCitation id="8FEFB3F7E6A5DAEF09324BFDA9353E74" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogenetic relationships of 116 isolates of the Alternaria species complex with related taxa with concatenated sequences of the SSU, LSU, ITS, GAPDH, RPB 2, TEF 1, Alt a 1, endoPG and OPA 10 - 2 loci using Bayesian inference (BI) and Maximum-likelihood (ML) methods. Bootstrap support values from ML ≥ 70 % and BI posterior values ≥ 0.9 are shown at nodes (ML / BI). Alternaria alternantherae CBS 124392 was the outgroup. * and red font indicates strains of this study. T indicates the ex-type strains, ET indicates the ex-epitype strains, HT indicates the ex-holotype strains." figureDoi="10.3897/mycokeys.101.115370.figure1" httpUri="https://binary.pensoft.net/fig/962733" pageId="0" pageNumber="1">1</figureCitation>
). Between
<taxonomicName id="67C93B5DCB1069D0E6B277B526D3A27D" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="B2B842FC37CDF2F4A7EB69ABF5C3DC85" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="4D6ECEB2BCB370CD2324FB6D48302A25" lsidName="A. dongshanqiaoensis" pageId="0" pageNumber="1" rank="species" species="dongshanqiaoensis">
<emphasis id="11AE3C58D21B1CBA9ED9FB522D210587" italics="true" pageId="0" pageNumber="1">A. dongshanqiaoensis</emphasis>
</taxonomicName>
DSQ2-2, there were 1/453 differences in Alt a1, 1/510 in ITS, 8/664 in OPA10-2, 1/401 in endoPG, 1/757 in RPB2, 1/996 in SSU and 3/293 in TEF1. Between
<taxonomicName id="3DC7841661A3CFAC39F12E0B73AA9950" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="0F55B7BB78CE6C2C63C914A5690817E5" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="99B08BF138AA2F85FE3C6A44030C5553" lsidName="A. citri" pageId="0" pageNumber="1" rank="species" species="citri">
<emphasis id="D5E7028AA7E56CB3696F2494BEB54141" italics="true" pageId="0" pageNumber="1">A. citri</emphasis>
</taxonomicName>
(ex-epitype, CBS 107.27), there were 1/453 differences in Alt a1, 3/510 in ITS, 8/664 in OPA10-2, 1/401 in endoPG, 1/996 in SSU and 3/293 in TEF1. Between
<taxonomicName id="EE3CDE1A8D33CD2AF0F162FFF83A6CA2" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="2061BC492E970F3EC5C7068D97CF8983" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="F835842EFB9A7C10BA69794F34A99E5B" lsidName="A. cinerariae" pageId="0" pageNumber="1" rank="species" species="cinerariae">
<emphasis id="97BCD7B8035D21EEE17ABB327596DB25" italics="true" pageId="0" pageNumber="1">A. cinerariae</emphasis>
</taxonomicName>
(ex-epitype, CBS 612.72), there were 1/453 differences in Alt a1, 3/510 in ITS, 8/664 in OPA10-2, 1/401 in endoPG, 1/996 in SSU and 3/293 in TEF1. Between
<taxonomicName id="90A518EE8680663B1D09976F51CDEED4" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="F93930E48909E4BF803D06183514F87C" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates and
<taxonomicName id="0250FC93BD1A0925E0E710BB9A8D491E" lsidName="A. kikuchiana" pageId="0" pageNumber="1" rank="species" species="kikuchiana">
<emphasis id="8599384EC4D53B31A235707B5455DDFF" italics="true" pageId="0" pageNumber="1">A. kikuchiana</emphasis>
</taxonomicName>
(ex-type, CBS 107.53), there were 3/453 differences in Alt a1, 3/510 in ITS, 2/401 in endoPG, 1/757 in RPB2, 1/996 in SSU and 3/293 in TEF1. The PHI analysis showed that there was no significant recombination between
<taxonomicName id="483ABE2F7287EFAB386A06AAA5B3AD96" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="396932F90579FAF5BE5730FFB8901196" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates and their related species (Φw = 0.1647) (Fig.
<figureCitation id="4E398F8B7B3AD794D5CC786375615EDF" captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Splitgraphs showing the results of the pairwise homoplasy index (PHI) test of newly described taxa and closely-related species using both LogDet transformation and splits decomposition A the PHI of Alternaria xinyangensis sp. nov. and A. dongshanqiaoensis sp. nov. with their phylogenetically related isolates or species B the PHI of A. shandongensis sp. nov., A. kunyuensis sp. nov., A. hunanensis sp. nov. and A. longqiaoensis sp. nov. with their phylogenetically related isolates or species C the PHI of A. cunninghamiicola sp. nov. with their phylogenetically-related isolates or species. PHI test value (Φw) &lt;0.05 indicate significant recombination within a dataset. * indicates strains of this study. T indicates the ex-type strains, ET indicates the ex-epitype strains, HT indicates the ex-holotype strains." figureDoi="10.3897/mycokeys.101.115370.figure2" httpUri="https://binary.pensoft.net/fig/962734" pageId="0" pageNumber="1">2A</figureCitation>
). Distinguishing characteristics of this new species and other similar species of
<taxonomicName id="3A889A3FAEE041F49C75D005C6A0CE96" class="Dothideomycetes" family="Moraceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="genus">
<emphasis id="B5293A028620A8F61DEDD2C511626D6A" italics="true" pageId="0" pageNumber="1">Alternaria</emphasis>
</taxonomicName>
spp. are shown in Table
<tableCitation id="84EAD06540F96B1BE052A34214F6807D" captionStart="Table 2" captionStartId="T2" captionText="Table 2. Distinguishing characteristics of the new species and similar known species of Alternaria spp. under growth conditionsa." httpUri="http://table.plazi.org/id/C175884AFAED43FDBFB74450B8D1CF39" pageId="0" pageNumber="1" tableUuid="C175884AFAED43FDBFB74450B8D1CF39">2</tableCitation>
. Morphologically, conidial number in chains of the
<taxonomicName id="3A68145A2331F59E19E2AA6124D49908" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="8426ECD53CC97C632A60F5A9DF82F328" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates were less than those of
<taxonomicName id="90B80694622FFC4B08F192278B4B03F6" lsidName="A. dongshanqiaoensis" pageId="0" pageNumber="1" rank="species" species="dongshanqiaoensis">
<emphasis id="D200FC56AB445A7064EB0AF6F3379841" italics="true" pageId="0" pageNumber="1">A. dongshanqiaoensis</emphasis>
</taxonomicName>
DSQ2-2 (2-7 conidia vs. 5-9 conidia). Conidia of the
<taxonomicName id="7F82EDA4F98DB3883F11D0E9220F867C" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="7FCC7470B1848AA48B9042542F51079A" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates were smaller than those of
<taxonomicName id="DAE2BAB693395A16187DAD80D44F9999" lsidName="A. citri" pageId="0" pageNumber="1" rank="species" species="citri">
<emphasis id="7E6A39A506FD967C54CDDD64B5BFAD11" italics="true" pageId="0" pageNumber="1">A. citri</emphasis>
</taxonomicName>
CBS 107.27 (ex-epitype) (19.9-31.8
<normalizedToken id="3C809F3B4AE65C5F1EEA888DDBB385DE" originalValue="×">x</normalizedToken>
8.6-12.9
<normalizedToken id="B4C99E08DBFD4B2766B85BDDC95A1D5D" originalValue="μm">μm</normalizedToken>
vs. 25-40
<normalizedToken id="768B6BE3068A87B49F0BF10571F58273" originalValue="×">x</normalizedToken>
15-25
<normalizedToken id="455BD55675C2CADA8CD2A71A918260A2" originalValue="μm">μm</normalizedToken>
) (
<bibRefCitation id="03053069D7F8F14E2A3C32C0C7FEB20A" DOI="https://doi.org/10.1086/328217" author="Pierce, NB" journalOrPublisher=")" pageId="0" pageNumber="1" pagination="234 - 235" refId="B59" refString="Pierce, NB, 1902. Black rot of oranges. Botanical Gazette (Chicago, Ill.) 33 (3): 234 - 235, DOI: https://doi.org/10.1086/328217" title="Black rot of oranges. Botanical Gazette (Chicago, Ill." url="https://doi.org/10.1086/328217" volume="33" year="1902">Pierce 1902</bibRefCitation>
). Secondary conidiophores of the
<taxonomicName id="B5660DF55D4C089A604B31E2ED83F29E" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="8BC7CC072AF40B18251F143E0D0CFBFF" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates were significantly shorter than those of
<taxonomicName id="2865277B3A2E567C4B110C28B1E4FB14" lsidName="A. cinerariae" pageId="0" pageNumber="1" rank="species" species="cinerariae">
<emphasis id="9BB73A7C74E1963CEE71E430B4D1A2D8" italics="true" pageId="0" pageNumber="1">A. cinerariae</emphasis>
</taxonomicName>
CBS 612.72 (ex-epitype) (5.3-16.0
<normalizedToken id="235F06AD1F5981F196D155154BC319A3" originalValue="×">x</normalizedToken>
2.8-4.1
<normalizedToken id="2138EAA5D8F9C4286DD7235190727CB5" originalValue="μm">μm</normalizedToken>
vs. 80-159
<normalizedToken id="17779CC5F78A0A3FE65A06227B4730C7" originalValue="×">x</normalizedToken>
5-9
<normalizedToken id="DDCD5B735B60034F59CEAD2EF1FEECB6" originalValue="μm">μm</normalizedToken>
) (
<bibRefCitation id="4990FC42F517B4E3565F6152F05ED792" DOI="https://doi.org/10.3114/fuse.2020.05.13" author="Nishikawa, J" journalOrPublisher="Fungal Systematics and Evolution" pageId="0" pageNumber="1" pagination="197 - 281" refId="B57" refString="Nishikawa, J, Nakashima, C, 2020. Japanese species of Alternaria and their species boundaries based on host range. Fungal Systematics and Evolution 5 (1): 197 - 281, DOI: https://doi.org/10.3114/fuse.2020.05.13" title="Japanese species of Alternaria and their species boundaries based on host range." url="https://doi.org/10.3114/fuse.2020.05.13" volume="5" year="2020">Nishikawa and Nakashima 2020</bibRefCitation>
). Conidia in chains of the
<taxonomicName id="7BA3CC71ED3C67BE254753F3C24CE1AA" lsidName="A. xinyangensis" pageId="0" pageNumber="1" rank="species" species="xinyangensis">
<emphasis id="13A1E1703AB783BFC5CB8817EB1C64C4" italics="true" pageId="0" pageNumber="1">A. xinyangensis</emphasis>
</taxonomicName>
isolates were less than those of
<taxonomicName id="DCBC82633EF94CF04340BF4BA44D5F35" lsidName="A. kikuchiana" pageId="0" pageNumber="1" rank="species" species="kikuchiana">
<emphasis id="D7BBC22B4B6B8047A8A9BC4A38145FCF" italics="true" pageId="0" pageNumber="1">A. kikuchiana</emphasis>
</taxonomicName>
CBS 107.53 (ex-type) (2-7 conidia vs. 6-9 conidia) (
<bibRefCitation id="114A98E7FBF3A451A82F979679A7A32D" DOI="https://doi.org/10.1016/j.myc.2018.05.003" author="Nishikawa, J" journalOrPublisher="Mycoscience" pageId="0" pageNumber="1" pagination="1 - 9" refId="B56" refString="Nishikawa, J, Nakashima, C, 2019. Morphological and molecular characterization of the strawberry black leaf spot pathogen referred to as the strawberry pathotype of Alternaria alternata. Mycoscience 60 (1): 1 - 9, DOI: https://doi.org/10.1016/j.myc.2018.05.003" title="Morphological and molecular characterization of the strawberry black leaf spot pathogen referred to as the strawberry pathotype of Alternaria alternata." url="https://doi.org/10.1016/j.myc.2018.05.003" volume="60" year="2019">Nishikawa and Nakashima 2019</bibRefCitation>
). In conclusion, the phylogenetic and morphological evidence supports this fungus as being a new species within the
<taxonomicName id="5F44F9DE89B8BF169463EB7F7461D46F" class="Dothideomycetes" family="Moraceae" genus="Alternaria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Alternaria alternata" order="Pleosporales" pageId="0" pageNumber="1" phylum="Ascomycota" rank="species" species="alternata">
<emphasis id="F337A7AAE85B011DD4585C6693EA23E4" italics="true" pageId="0" pageNumber="1">Alternaria alternata</emphasis>
</taxonomicName>
species complex.
</paragraph>
</subSubSection>
</treatment>
</document>