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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.84.71701" ID-GBIF-Dataset="230e5bf5-63a7-40b8-97d5-6b5c2f835d8a" ID-PMC="PMC8545784" ID-Pensoft-Pub="1314-4049-84-15" ID-Pensoft-UUID="D929207D8DC95D33AF0CBBBAE2BE14C1" ID-PubMed="34720645" ModsDocID="1314-4049-84-15" checkinTime="1634632126309" checkinUser="pensoft" docAuthor="Yang, Qin, Tang, Jie &amp; Zhou, Guo Y." docDate="2021" docId="22A1496A2AD05002A1D77888283CD8FC" docLanguage="en" docName="MycoKeys 84: 15-33" docOrigin="MycoKeys 84" docPubDate="2021-10-18" docSource="http://dx.doi.org/10.3897/mycokeys.84.71701" docTitle="Diaporthe hubeiensis Dissanayake, X. H. Li &amp; K. D. Hyde" docType="treatment" docVersion="4" id="D929207D8DC95D33AF0CBBBAE2BE14C1" lastPageNumber="15" masterDocId="D929207D8DC95D33AF0CBBBAE2BE14C1" masterDocTitle="Characterization of Diaporthe species on Camellia oleifera in Hunan Province, with descriptions of two new species" masterLastPageNumber="33" masterPageNumber="15" pageNumber="15" updateTime="1668137276081" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Characterization of Diaporthe species on Camellia oleifera in Hunan Province, with descriptions of two new species</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Qin</mods:namePart>
<mods:affiliation>Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China &amp; The Key Laboratory for Non-Wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tang, Jie</mods:namePart>
<mods:affiliation>Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhou, Guo Y.</mods:namePart>
<mods:affiliation>Forestry Biotechnology Hunan Key Laboratories, Central South University of Forestry and Technology, Changsha 410004, China &amp; The Key Laboratory for Non-Wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China</mods:affiliation>
<mods:nameIdentifier type="email">zgyingqq@163.com</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2021</mods:date>
<mods:detail type="pubDate">
<mods:number>2021-10-18</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>84</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>15</mods:start>
<mods:end>33</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.84.71701</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.84.71701</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-84-15</mods:identifier>
<mods:identifier type="Pensoft-UUID">D929207D8DC95D33AF0CBBBAE2BE14C1</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="189468395" LSID="urn:lsid:plazi:treatment:22A1496A2AD05002A1D77888283CD8FC" httpUri="http://treatment.plazi.org/id/22A1496A2AD05002A1D77888283CD8FC" lastPageNumber="15" pageId="0" pageNumber="15">
<subSubSection pageId="0" pageNumber="15" type="nomenclature">
<paragraph pageId="0" pageNumber="15">
<taxonomicName LSID="22A1496A-2AD0-5002-A1D7-7888283CD8FC" authority="Dissanayake, X. H. Li &amp; K. D. Hyde" authorityName="Dissanayake, X. H. Li &amp; K. D. Hyde" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe hubeiensis" order="Diaporthales" pageId="0" pageNumber="15" phylum="Ascomycota" rank="species" species="hubeiensis">Diaporthe hubeiensis Dissanayake, X.H. Li &amp; K.D. Hyde</taxonomicName>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="15" type="description">
<paragraph pageId="0" pageNumber="15">
<figureCitation captionStart="Figure 3" captionStartId="F4" captionText="Figure 3. Diaporthe hubeiensis (HNZZ 019) A Culture on PDA B conidiomata C conidiogenous cells D alpha conidia. Scale bars: 500 μm (B); 10 μm (C-D)." figureDoi="10.3897/mycokeys.84.71701.figure3" httpUri="https://binary.pensoft.net/fig/600650" pageId="0" pageNumber="15">Figure 3</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="15" type="reference_group">
<paragraph pageId="0" pageNumber="15">
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe hubeiensis" order="Diaporthales" pageId="0" pageNumber="15" phylum="Ascomycota" rank="species" species="hubeiensis">Diaporthe hubeiensis</taxonomicName>
Manawasinghe, Dissanayake, Li, Liu, Wanasinghe, Xu, Zhao, Zhang, Zhou, Hyde, Brooks &amp; Yan, Frontiers in Microbiology 10(no. 1936): 20 (2019)
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="15" type="description">
<paragraph pageId="0" pageNumber="15">Description.</paragraph>
<paragraph pageId="0" pageNumber="15">
Asexual morph:
<emphasis italics="true" pageId="0" pageNumber="15">pycnidia</emphasis>
on PDA in culture, 700-885
<normalizedToken originalValue="μm">μm</normalizedToken>
in diam., superficial, scattered, dark brown to black, globose or subglobose.
<emphasis italics="true" pageId="0" pageNumber="15">Conidiophores</emphasis>
reduced to conidiogenous cells.
<emphasis italics="true" pageId="0" pageNumber="15">Conidiogenous cells</emphasis>
(6.5-)7-10(-11.5)
<normalizedToken originalValue="×">x</normalizedToken>
2-3.5
<normalizedToken originalValue="μm">μm</normalizedToken>
(n = 30), aseptate, cylindrical, phiailidic, straight or slightly curved.
<emphasis italics="true" pageId="0" pageNumber="15">Alpha conidia</emphasis>
5.8-8(-8.5)
<normalizedToken originalValue="×">x</normalizedToken>
2.5-3.2
<normalizedToken originalValue="μm">μm</normalizedToken>
(n = 30), aseptate, hyaline, ellipsoidal to cylindrical, biguttulate, blunt at both ends.
<emphasis italics="true" pageId="0" pageNumber="15">Beta conidia</emphasis>
not observed.
</paragraph>
<caption doi="10.3897/mycokeys.84.71701.figure3" httpUri="https://binary.pensoft.net/fig/600650" pageId="0" pageNumber="15" start="Figure 3" startId="F4">
<paragraph pageId="0" pageNumber="15">
<emphasis bold="true" pageId="0" pageNumber="15">Figure 3.</emphasis>
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe hubeiensis" order="Diaporthales" pageId="0" pageNumber="15" phylum="Ascomycota" rank="species" species="hubeiensis">
<emphasis italics="true" pageId="0" pageNumber="15">Diaporthe hubeiensis</emphasis>
</taxonomicName>
(HNZZ019)
<emphasis bold="true" pageId="0" pageNumber="15">A</emphasis>
Culture on PDA
<emphasis bold="true" pageId="0" pageNumber="15">B</emphasis>
conidiomata
<emphasis bold="true" pageId="0" pageNumber="15">C</emphasis>
conidiogenous cells
<emphasis bold="true" pageId="0" pageNumber="15">D</emphasis>
alpha conidia. Scale bars: 500
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="15">B</emphasis>
); 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="15">C-D</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="15" type="description">
<paragraph pageId="0" pageNumber="15">Culture characters.</paragraph>
<paragraph pageId="0" pageNumber="15">Culture incubated on PDA at 25 °C, originally flat with white felted aerial mycelium, becoming dark brown mycelium due to pigment formation, conidiomata irregularly distributed over agar surface after 20 days.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="15" type="materials_examined">
<paragraph pageId="0" pageNumber="15">Specimens examined.</paragraph>
<paragraph pageId="0" pageNumber="15">
<materialsCitation collectingDate="2020-08-14" collectorName="Q. Yang" country="China" latitude="27.043055" location="Zhuzhou City" longLatPrecision="20" longitude="113.32222" specimenCount="1" stateProvince="Hunan Province">
<collectingCountry name="China">China</collectingCountry>
.
<collectingRegion country="China" name="Hunan">Hunan Province</collectingRegion>
:
<location LSID="urn:lsid:plazi:treatment:22A1496A2AD05002A1D77888283CD8FC:91C9E8A0E76ABC38838218C9F6CDB801" country="China" latitude="27.043055" longLatPrecision="20" longitude="113.32222" name="Zhuzhou City" stateProvince="Hunan Province">Zhuzhou City</location>
, on leaves of
<taxonomicName class="Magnoliopsida" family="Theaceae" genus="Camellia" kingdom="Plantae" lsidName="Camellia oleifera" order="Ericales" pageId="0" pageNumber="15" phylum="Tracheophyta" rank="species" species="oleifera">
<emphasis italics="true" pageId="0" pageNumber="15">Camellia oleifera</emphasis>
</taxonomicName>
,
<geoCoordinate degrees="27" direction="north" minutes="2" orientation="latitude" precision="15" seconds="35" value="27.043055">27°2'35&quot;N</geoCoordinate>
,
<geoCoordinate degrees="113" direction="east" minutes="19" orientation="longitude" precision="15" seconds="20" value="113.32222">113°19'20&quot;E</geoCoordinate>
,
<collectingDate value="2020-08-14">14 Aug. 2020</collectingDate>
,
<emphasis italics="true" pageId="0" pageNumber="15">
<collectorName>Q. Yang</collectorName>
</emphasis>
(CSUFT019; living cultures: HNZZ019 and HNZZ009)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="15" type="notes">
<paragraph pageId="0" pageNumber="15">Notes.</paragraph>
<paragraph pageId="0" pageNumber="15">
<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe hubeiensis" order="Diaporthales" pageId="0" pageNumber="15" phylum="Ascomycota" rank="species" species="hubeiensis">
<emphasis italics="true" pageId="0" pageNumber="15">Diaporthe hubeiensis</emphasis>
</taxonomicName>
was originally described as pathogen of grapevines in Hubei Province, China (
<bibRefCitation DOI="https://doi.org/10.3389/fmicb.2019.01936" author="Manawasinghe, IS" journalOrPublisher="Phytopathologia Mediterranea" pageId="0" pageNumber="15" refId="B24" refString="Manawasinghe, IS, Dissanayake, AJ, Li, X, Liu, M, Wanasinghe, DN, Xu, J, Zhao, W, Zhang, W, Zhou, Y, Hyde, KD, Brooks, S, Yan, J, 2019. High genetic diversity and species complexity of Diaporthe associated with grapevine dieback in China. Frontiers in Microbiology 10: e1936. https://doi.org/10.3389/fmicb.2019.01936" title="High genetic diversity and species complexity of Diaporthe associated with grapevine dieback in China. Frontiers in Microbiology 10: e 1936." url="https://doi.org/10.3389/fmicb.2019.01936" year="2019">Manawasinghe et al. 2019</bibRefCitation>
). In the present study, two isolates (HNZZ019 and HNZZ009) are closely related to
<taxonomicName lsidName="D. hubeiensis" pageId="0" pageNumber="15" rank="species" species="hubeiensis">
<emphasis italics="true" pageId="0" pageNumber="15">D. hubeiensis</emphasis>
</taxonomicName>
in the combined phylogenetic tree (Fig.
<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram of Diaporthe resulting from a maximum likelihood analysis based on combined ITS, cal, his 3, tef 1 and tub 2. Numbers above the branches indicate ML bootstraps (left, ML BS ≥ 50 %) and Bayesian Posterior Probabilities (right, BPP ≥ 0.75). The tree is rooted with Diaporthella corylina. Isolates in current study are in blue. &quot; - &quot; indicates ML BS &lt;50 % or BI PP &lt;0.75." figureDoi="10.3897/mycokeys.84.71701.figure1" httpUri="https://binary.pensoft.net/fig/600647" pageId="0" pageNumber="15">1</figureCitation>
). The differences of nucleotides in the concatenated alignment (1/460 in ITS, 3/458 in
<emphasis italics="true" pageId="0" pageNumber="15">cal</emphasis>
, 1/320 in
<emphasis italics="true" pageId="0" pageNumber="15">his3</emphasis>
and 3/433 in
<emphasis italics="true" pageId="0" pageNumber="15">tub2</emphasis>
) are minor. Morphological comparison indicated that the isolates were similar to
<taxonomicName lsidName="D. hubeiensis" pageId="0" pageNumber="15" rank="species" species="hubeiensis">
<emphasis italics="true" pageId="0" pageNumber="15">D. hubeiensis</emphasis>
</taxonomicName>
by the size of alpha conidia. We therefore identify the isolates as belonging to
<taxonomicName lsidName="D. hubeiensis" pageId="0" pageNumber="15" rank="species" species="hubeiensis">
<emphasis italics="true" pageId="0" pageNumber="15">D. hubeiensis</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</document>