105 lines
10 KiB
XML
105 lines
10 KiB
XML
<document id="0279DA1246FB1D29558FE55088D6E398" ID-DOI="10.1126/science.1137614" ID-GBIF-Dataset="556b96ba-30e1-43c3-96ec-6a2c43943e48" ID-Zenodo-Dep="3744436" IM.metadata_requiresApprovalFor="plazi" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_requiresApprovalFor="plazi" checkinTime="1586343966378" checkinUser="jeremy" docAuthor="John M. Asara, Mary H. Schweitzer, Lisa M. Freimark, Matthew Phillips & Lewis C. Cantley" docDate="2007" docId="CA36370BFFCEFFD5F28FFDC2F692FE3F" docLanguage="en" docName=" Asraetal2007ABBYY.pdf.imd" docOrigin="Science 316 (5822)" docStyle="DocumentStyle{}" docTitle="Tyrannosaurus rex" docType="treatment" docVersion="4" lastPageNumber="281" masterDocId="360F4F73FFCCFFD6F475FFCDFFE5FF8C" masterDocTitle="Protein sequences from mastodon and Tyrannosaurus rex revealed by mass spectrometry" masterLastPageNumber="284" masterPageNumber="280" pageNumber="281" updateTime="1698736454845" updateUser="plazi">
|
|
<mods:mods id="B1388A9E2A6A5D71A704BBAD7A578901" xmlns:mods="http://www.loc.gov/mods/v3">
|
|
<mods:titleInfo id="64EF7B66B91BA3097E8AF8849005F497">
|
|
<mods:title id="0FF2370CEE2D4F0873DCE73B39C8CFCB">Protein sequences from mastodon and Tyrannosaurus rex revealed by mass spectrometry</mods:title>
|
|
</mods:titleInfo>
|
|
<mods:name id="01FEB60E757CD2844F856B8C361D7514" type="personal">
|
|
<mods:role id="425FD8702A0FB18A72229586321CCE6A">
|
|
<mods:roleTerm id="00D23D7128D2FAB7721F4BD40AF11853">Author</mods:roleTerm>
|
|
</mods:role>
|
|
<mods:namePart id="6C9185532E2C81628C0F788814D2849A">John M. Asara</mods:namePart>
|
|
<mods:affiliation id="041DABB08A13348838D7AAF874DA47F1">division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA. department of Pathology, Harvard Medical School, Boston, MA 02115, USA; North Carolina Museum of Natural Sciences, Raleigh, NC 27601, USA; and Museum of the Rockies, Montana State University, Bozeman, MT 59717, USA.</mods:affiliation>
|
|
<mods:nameIdentifier id="5BD1D2EBF3B040EF9C462F5E2C5BF621" type="email">jasara@bidmc.harvard.edu</mods:nameIdentifier>
|
|
</mods:name>
|
|
<mods:name id="6C4F8BA5EAB9493785D23355ED86308D" type="personal">
|
|
<mods:role id="B5345D652206E2B96C96AC56327B932B">
|
|
<mods:roleTerm id="E3CE17BBA41CB28126CE5BD359247C2A">Author</mods:roleTerm>
|
|
</mods:role>
|
|
<mods:namePart id="38A53D9AFF9DF9B61C667AA3B775C2F8">Mary H. Schweitzer</mods:namePart>
|
|
<mods:affiliation id="9A17AEB8CEE51D405B534BC2B4DEB35B">department of Marine, Earth and Atmospheric Sciences, North Carolina State University, Raleigh, NC 27695, USA.</mods:affiliation>
|
|
</mods:name>
|
|
<mods:name id="1D638D225B40B88943DC14B1F717A9C7" type="personal">
|
|
<mods:role id="D2D73F60F18717917A07BFE799A27E32">
|
|
<mods:roleTerm id="CCFC59CDBB0188CD68B6BC24388A8305">Author</mods:roleTerm>
|
|
</mods:role>
|
|
<mods:namePart id="ADFD2C0492DFD37CEA4B81A3504E3DF2">Lisa M. Freimark</mods:namePart>
|
|
<mods:affiliation id="81A8A9825DB9436199D1846D160628C4">division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.</mods:affiliation>
|
|
</mods:name>
|
|
<mods:name id="4DB466EBDDD42723E6F941497420B885" type="personal">
|
|
<mods:role id="8D316F7CDC1C52B246718116494FA172">
|
|
<mods:roleTerm id="BF5EC8AAC31C3373BF65E48C6083CEB7">Author</mods:roleTerm>
|
|
</mods:role>
|
|
<mods:namePart id="4D3993F376604816A63048A1DA0ACCD6">Matthew Phillips</mods:namePart>
|
|
<mods:affiliation id="4C7EE15A46095A5B8B869A1CE72E7637">division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.</mods:affiliation>
|
|
</mods:name>
|
|
<mods:name id="12026F235B7CAD1F46A63D15CAE98951" type="personal">
|
|
<mods:role id="188EC5B4E1756D82885943600B967055">
|
|
<mods:roleTerm id="7CA458BC76F219F793CA4B2CA2A3EA60">Author</mods:roleTerm>
|
|
</mods:role>
|
|
<mods:namePart id="30DA990B31B23E8A917D0554C0B6768B">Lewis C. Cantley</mods:namePart>
|
|
<mods:affiliation id="19B82CAC923AD9C06FFD11246DCA596A">division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA. department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.</mods:affiliation>
|
|
</mods:name>
|
|
<mods:typeOfResource id="733DD53650A4F9CB6CF41192E62AD5CA">text</mods:typeOfResource>
|
|
<mods:relatedItem id="3D78E65FD6B2A5FA30D19E361669B9C7" type="host">
|
|
<mods:titleInfo id="F0E0915290D7F4A0DE2BF875019BB183">
|
|
<mods:title id="6653B34CFD3241E495833BB02E85E914">Science</mods:title>
|
|
</mods:titleInfo>
|
|
<mods:part id="B1E5487084BDD828B52315697186677E">
|
|
<mods:date id="AAF11DFBB823FEF18C23F0B28A6434C5">2007</mods:date>
|
|
<mods:detail id="FF07E1247EF5519B4EE0E658164ECD6F" type="pubDate">
|
|
<mods:number id="F8B23263253C732FDA76B19347AF9E01">2007-04-13</mods:number>
|
|
</mods:detail>
|
|
<mods:detail id="9632F6F4F8A8B11A3BFC5F40AEAC4151" type="volume">
|
|
<mods:number id="279DBCAF53C3EABEFF8F6BE21E6904BA">316</mods:number>
|
|
</mods:detail>
|
|
<mods:detail id="6F0BA6B48AFC1FFF027C8AEC646AC583" type="issue">
|
|
<mods:number id="28C003672C6B5D49E30E601618B49311">5822</mods:number>
|
|
</mods:detail>
|
|
<mods:extent id="F46E5EC0689957BF2FCB09CB4678A1AE" unit="page">
|
|
<mods:start id="D40B9DFB47C3F080272679D70E7201FC">280</mods:start>
|
|
<mods:end id="D7DC7418844502402FF73FF7AD504168">284</mods:end>
|
|
</mods:extent>
|
|
</mods:part>
|
|
</mods:relatedItem>
|
|
<mods:classification id="1A9A48BB803E5097324597F381B39028">journal article</mods:classification>
|
|
<mods:identifier id="E3E77D67D18F2CC29F184354C6B4499F" type="DOI">10.1126/science.1137614</mods:identifier>
|
|
<mods:identifier id="A274D7224CC467CA457FF4F2B8F9C42E" type="GBIF-Dataset">556b96ba-30e1-43c3-96ec-6a2c43943e48</mods:identifier>
|
|
<mods:identifier id="7285B4E84C91763C09EEDADAC5BC1DCC" type="Zenodo-Dep">3744436</mods:identifier>
|
|
</mods:mods>
|
|
<treatment id="CA36370BFFCEFFD5F28FFDC2F692FE3F" ID-DOI="http://doi.org/10.5281/zenodo.3809611" ID-GBIF-Taxon="184571765" ID-Zenodo-Dep="3809611" LSID="urn:lsid:plazi:treatment:CA36370BFFCEFFD5F28FFDC2F692FE3F" httpUri="http://treatment.plazi.org/id/CA36370BFFCEFFD5F28FFDC2F692FE3F" lastPageId="3" lastPageNumber="281" pageId="2" pageNumber="281">
|
|
<subSubSection id="0A85D596FFCEFFD4F28FFDC2F78BFC84" pageId="2" pageNumber="281" type="nomenclature">
|
|
<paragraph id="4220861DFFCEFFD4F28FFDC2F78BFC84" blockId="2.[1735,2432,349,1302]" pageId="2" pageNumber="281">
|
|
We sequenced collagen protein fragments derived from fossilized bones of two extinct taxa: a 160,000- to 600,000-year-old mastodon [specimen number Museum of the Rockies (MOR) 605] (
|
|
<bibRefCitation id="260EFBECFFCEFFD4F3C7FD73F820FD50" author="C. L. Hill" box="[1970,1989,702,732]" journalOrPublisher="Quat. Int." pageId="2" pageNumber="281" pagination="87" part="142 - 143" refId="ref4605" refString="9. C. L. Hill, Quat. Int. 142 - 143, 87 (2006)." type="journal article" year="2006">9</bibRefCitation>
|
|
) and a 68-million-year-old dinosaur
|
|
<emphasis id="70EB5A0FFFCEFFD4F325FD27F78BFC84" box="[1872,2158,746,776]" italics="true" pageId="2" pageNumber="281">
|
|
(
|
|
<taxonomicName id="859FFD9EFFCEFFD4F32FFD27F78CFC84" authority="Osborn, 1905" box="[1882,2153,746,776]" class="Reptilia" family="Tyrannosauridae" genus="Tyrannosaurus" kingdom="Animalia" order="Dinosauria" pageId="2" pageNumber="281" phylum="Chordata" rank="species" species="rex">Tyrannosaurus rex</taxonomicName>
|
|
,
|
|
</emphasis>
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection id="0A85D596FFCEFFD4FC08FD27F699FC84" box="[2173,2428,746,776]" pageId="2" pageNumber="281" type="materials_examined">
|
|
<paragraph id="4220861DFFCEFFD4FC08FD27F699FC84" blockId="2.[1735,2432,349,1302]" box="[2173,2428,746,776]" pageId="2" pageNumber="281">
|
|
<materialsCitation id="F2F78C40FFCEFFD4FC08FD27F6C0FC84" ID-GBIF-Occurrence="3335061301" box="[2173,2341,746,776]" collectionCode="MOR" pageId="2" pageNumber="281" specimenCode="MOR 1125">MOR 1125</materialsCitation>
|
|
)
|
|
<emphasis id="70EB5A0FFFCEFFD4FD49FD27F699FC84" box="[2364,2428,746,776]" italics="true" pageId="2" pageNumber="281">
|
|
(
|
|
<bibRefCitation id="260EFBECFFCEFFD4FD32FD27F689FC84" author="M. H. Schweitzer & J. L. Wittmeyer & J. R. Horner & J. K. Toporski" box="[2375,2412,746,776]" journalOrPublisher="Science" pageId="2" pageNumber="281" pagination="1952" part="307" refId="ref4626" refString="10. M. H. Schweitzer, J. L. Wittmeyer, J. R. Horner, J. K. Toporski, Science 307, 1952 (2005)." type="journal article" year="2005">10</bibRefCitation>
|
|
),
|
|
</emphasis>
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection id="0A85D596FFCEFFD5F2BCFCDBF692FE3F" lastPageId="3" lastPageNumber="282" pageId="2" pageNumber="281" type="discussion">
|
|
<paragraph id="4220861DFFCEFFD5F2BCFCDBF692FE3F" blockId="2.[1735,2432,349,1302]" lastBlockId="3.[1754,2453,359,1398]" lastPageId="3" lastPageNumber="282" pageId="2" pageNumber="281">
|
|
results that are supported by immunological and molecular analyses published in this issue by Schweitzer
|
|
<emphasis id="70EB5A0FFFCEFFD4F318FCA0F818FC07" box="[1901,2045,877,907]" italics="true" pageId="2" pageNumber="281">
|
|
et al. (
|
|
<bibRefCitation id="260EFBECFFCEFFD4F3BEFCA0F80BFC07" author="M. H. Schweitzer" box="[1995,2030,877,907]" journalOrPublisher="Science" pageId="2" pageNumber="281" pagination="277" part="316" refId="ref4660" refString="11. M. H. Schweitzer et al., Science 316, 277 (2007)." type="journal article" year="2007">11</bibRefCitation>
|
|
).
|
|
</emphasis>
|
|
We first looked for tryptic peptide fragments from extracts of fossilized bone that matched identically with sequences from an orthologous protein or proteins from extant taxa, thereby identifying the protein(s) of interest. This is a common procedure for conserved proteins from taxa that share genomic information. Next, we generated a protein sequence database of likely drifts in amino acids in other tryptic peptides by comparing amino acid sequences ofthe orthologs from multiple related extant taxa. This approach produced a manageable number of theoretical protein sequences. The predicted peptide fragmentation pattern from these theoretical protein sequences were then compared with the fragmentation patterns of additional peptides derived from extracts of fossilized bone that did not match peptides in public sequence databases (fig. S1).
|
|
</paragraph>
|
|
</subSubSection>
|
|
</treatment>
|
|
</document> |