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<taxonomicName id="A6E8D62987DB584D11773B351F7B1F02" authority="Thor, 1898" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" infraOrder="Hydrachnidia" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="15EFC458BE5E304272D548CA4E3DEF47" italics="true">Hygrobates longiporus</emphasis>
<bibRefCitation id="306A6A4F3B12A225E030297387970CEC" author="Thor S" journalOrPublisher="Archiv for Mathematik og Naturvidenskab" pagination="1-12" refId="B48" refString="Thor S (1898) Nye hydrachnideformer, fundne i Norge sommeren 1898, Forelobig meddelelse. Archiv for Mathematik og Naturvidenskab 20: 1 - 12." title="Nye hydrachnideformer, fundne i Norge sommeren 1898, Forelobig meddelelse." volume="20" year="1898">Thor, 1898</bibRefCitation>
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complex
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<figureCitation id="6E34D20EB679516EED22A54FCC1DC222" captionStart="Figure 7" captionStartId="F7" captionText="Figure 7. Neighbor-Joining tree of the Hygrobates longiporus complex obtained from 53 nucleotide COI sequences; 23 sequences were taken from Pešić et al. (2022 a, 2023 f), 26 sequences from Portugal are newly generated in this study, and four private sequences (HYDIR 005-23, HYDIR 006-23, HYDIR 019-23, HYDIR 020-23) from Turkey were directly taken from the BOLD. Hygrobates balcanicus from Portugal was used as outgroup. BINs are based on the barcode analysis from 16 May 2024. Country codes: FR = France, GR = Greece, IR = Iran, NO = Norway, PT = Portugal, RS = Serbia, TR = Turkey. Bootstrap values &gt; 50 % from 1000 bootstrap replicates on branches." figureDoi="10.3897/zookeys.1217.131730.figure7" httpUri="https://binary.pensoft.net/fig/1164871">Fig. 7</figureCitation>
</paragraph>
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<heading id="8B3976510EEE28B88D08972CD594EACE" reason="title">Material examined.</heading>
</paragraph>
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,
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: •
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,
<elevation id="CCEC95DC739D36076AEA84AD71F27854" metricMagnitude="2" metricUnit="m" metricValue="3.12" unit="m" value="312.0">
<quantity id="7A25D079EA95A6F9FF33FCFCF9136175" metricMagnitude="2" metricUnit="m" metricValue="3.12" unit="m" value="312.0">312 m</quantity>
a. s. l.
</elevation>
,
<date id="467236C9DAE9599BEED1D080DCFA7C8E" value="2023-08-23">
<collectingDate id="BEBB1C8D8A48172F7EC2F4D900563739" value="2023-08-23">23 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="B397AA8B42983D5DE66A166F44E8E7D8">Ferreira</collectorName>
,
<collectorName id="ADB0EDBDCA9C192DD28705E5B92D2836">Benitez-Bosco</collectorName>
&amp;
<collectorName id="0BEC252488992DD0010E833930EDB3FD">Padilha</collectorName>
,
<specimenCount id="6418A2C9F0377B71E2EC17068B0EE573" count="1" type="female">1 ♀</specimenCount>
, 1 deutonymph (sequenced)
</materialsCitation>
; •
<materialsCitation id="DAAA51EF1309B2DCE95AF80AD2C5A81A" collectingDate="2023-08-24" collectorName="Ferreira &amp; Benitez-Bosco &amp; Padilha" country="Portugal" county="Seia" elevation="1021" latitude="40.401" location="Praia Fluvial de Sabugueiro" longLatPrecision="427" longitude="-7.64" municipality="Rio Alva" specimenCount="3" specimenCount-female="1" specimenCount-male="2" stateProvince="Guarda">
<collectingCounty id="B53A8C2056103623803202B17DECA471">Seia</collectingCounty>
,
<collectingMunicipality id="E62B44731BA9D4C62DCF6977CF0CE7E8">Rio Alva</collectingMunicipality>
,
<location id="B8D009C43C5A13A400742DB085EDD496" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:B8D009C43C5A13A400742DB085EDD496" country="Portugal" county="Seia" latitude="40.401" longLatPrecision="427" longitude="-7.64" municipality="Rio Alva" name="Praia Fluvial de Sabugueiro" stateProvince="Guarda">Praia Fluvial de Sabugueiro</location>
, river,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0EDGBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.640000,40.401000]}">
<geoCoordinate id="C9FEAA3B8810066F99D43AE9AED0A033" degrees="40.401" direction="north" orientation="latitude" precision="55" value="40.401">40.401 ° N</geoCoordinate>
,
<geoCoordinate id="FC63BBCEA6AF0AEAAE2EF4EBFE503949" degrees="7.64" direction="west" orientation="longitude" precision="555" value="-7.64">7.64 ° W</geoCoordinate>
</named-content>
,
<elevation id="01E3A021F8CE50AC25D356B7BEDB5AFC" metricMagnitude="3" metricUnit="m" metricValue="1.021" unit="m" value="1021.0">
<quantity id="F96C1CBA4FCC941102F19EF60BACD435" metricMagnitude="3" metricUnit="m" metricValue="1.021" unit="m" value="1021.0">1021 m</quantity>
a. s. l.
</elevation>
,
<date id="D0A0AA30473EF5C06933027CFEAB38AD" value="2023-08-24">
<collectingDate id="DEEAF8FE13B9CA53D87A21A40E88BC8C" value="2023-08-24">24 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="B5E8361D9E9F88936F073157D667E1C4">Ferreira</collectorName>
,
<collectorName id="3E77FF581EE44B54F7986AF176A7B21A">Benitez-Bosco</collectorName>
&amp;
<collectorName id="8DAA8D287084AD0467E5EF92A78BA59A">Padilha</collectorName>
,
<specimenCount id="918AD36D9AF3B8DFF5A4D5DA3A833049" count="2" type="male">2 ♂</specimenCount>
,
<specimenCount id="65E0D22D08F87E8FBE9D7F97C5A21D14" count="1" type="female">1 ♀</specimenCount>
, 1 deutonymph (sequenced)
</materialsCitation>
; •
<materialsCitation id="00353C8BFD5E33C8CC282F78C8977920" collectingDate="2023-08-21" collectorName="Ferreira &amp; Benitez-Bosco &amp; Padilha" country="Portugal" elevation="1078" latitude="40.373" location="Poco do Inferno" longLatPrecision="69" longitude="-7.516" municipality="Manteigas" specimenCount="2" specimenCount-female="1" specimenCount-male="1" stateProvince="Guarda">
<collectingMunicipality id="1DA709895B8445ACE8AD22D12A40828F">Manteigas</collectingMunicipality>
,
<location id="3D9F14BA3D36D2B7A6C84B0A6742063A" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:3D9F14BA3D36D2B7A6C84B0A6742063A" country="Portugal" latitude="40.373" longLatPrecision="69" longitude="-7.516" municipality="Manteigas" name="Poco do Inferno" stateProvince="Guarda">Poço do Inferno</location>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0ELGBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.516000,40.373000]}">
<geoCoordinate id="DA693B62E848BDC0BF90924126F3EE5A" degrees="40.373" direction="north" orientation="latitude" precision="55" value="40.373">40.373 ° N</geoCoordinate>
,
<geoCoordinate id="C91AD1473F86F0ABE243C328FDB38FA6" degrees="7.516" direction="west" orientation="longitude" precision="55" value="-7.516">7.516 ° W</geoCoordinate>
</named-content>
,
<elevation id="A987A679F3379107A146281A6124AD79" metricMagnitude="3" metricUnit="m" metricValue="1.0779999999999998" unit="m" value="1078.0">
<quantity id="110B32B5AD006440824C5AB5B6007C55" metricMagnitude="3" metricUnit="m" metricValue="1.0779999999999998" unit="m" value="1078.0">1078 m</quantity>
a. s. l.
</elevation>
,
<date id="0D4682408F3CC728F9B345406D13B51E" value="2023-08-21">
<collectingDate id="BC4009895A9BD36E65818D48ED500558" value="2023-08-21">21 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="C54F0D20EFA4B2B584A53FE0C12E4845">Ferreira</collectorName>
,
<collectorName id="5DDB4C0126A1F2D3FF0A28F9D4731E0F">Benitez-Bosco</collectorName>
&amp;
<collectorName id="449403AAA8C6FF3AA00FA760918D00FF">Padilha</collectorName>
,
<specimenCount id="0BDD313B2CC35CA3812974C77CF7F796" count="1" type="male">1 ♂</specimenCount>
,
<specimenCount id="8D3D46C119868332B5DA9FDE67E886B0" count="1" type="female">1 ♀</specimenCount>
(sequenced)
</materialsCitation>
; •
<materialsCitation id="33FAE15DBA32BCE53E2B008ADCFD5D1A" collectingDate="2023-08-24" collectorName="Ferreira &amp; Benitez-Bosco &amp; Padilha" country="Portugal" county="Manteigas" elevation="999" latitude="40.443" location="Covao" longLatPrecision="69" longitude="-7.514" municipality="Mondego" specimenCount="4" specimenCount-female="3" specimenCount-male="1" stateProvince="Guarda">
<collectingCounty id="60D4F3C0A176E9A50B0B9ACD7134983C">Manteigas</collectingCounty>
,
<collectingMunicipality id="5B640E72CF4BA613C42D298894FE2E20">Mondego</collectingMunicipality>
,
<location id="48D07AF8358CCA1549FADBBFAD8EFB92" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:48D07AF8358CCA1549FADBBFAD8EFB92" country="Portugal" county="Manteigas" latitude="40.443" longLatPrecision="69" longitude="-7.514" municipality="Mondego" name="Covao" stateProvince="Guarda">Covão</location>
da ponte,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0ETGBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.514000,40.443000]}">
<geoCoordinate id="5AC263BC6C86B5D489DAA0C86C83A3EF" degrees="40.443" direction="north" orientation="latitude" precision="55" value="40.443">40.443 ° N</geoCoordinate>
,
<geoCoordinate id="3B4FE500DF7956BB83736E8CB7A360DA" degrees="7.514" direction="west" orientation="longitude" precision="55" value="-7.514">7.514 ° W</geoCoordinate>
</named-content>
,
<elevation id="9DF3F5B87D4C922E696E114F771BCE00" metricMagnitude="2" metricUnit="m" metricValue="9.99" unit="m" value="999.0">
<quantity id="FE68879ED4A0A379110C7173206C4628" metricMagnitude="2" metricUnit="m" metricValue="9.99" unit="m" value="999.0">999 m</quantity>
a. s. l.
</elevation>
,
<date id="32FB0849C8E762ADC241702780CA76C0" value="2023-08-24">
<collectingDate id="ECB817F5AC1687DAA16905E4C1F866B5" value="2023-08-24">24 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="BFD03DD7EA0F00A1BC69A624DDDB6A56">Ferreira</collectorName>
,
<collectorName id="BE4BF84044DD3124D0A2FD51324B4322">Benitez-Bosco</collectorName>
&amp;
<collectorName id="A6EBB4021765FCFF4120BF7E9EBEA6A0">Padilha</collectorName>
,
<specimenCount id="4C1D95FF70C454E4BA8CDC02BEE38ED9" count="1" type="male">1 ♂</specimenCount>
,
<specimenCount id="E1E6AE5EBC31312DC6A7A427F90E0F45" count="3" type="female">3 ♀</specimenCount>
, 2 deutonymph (sequenced)
</materialsCitation>
; •
<materialsCitation id="5498FE1125C1A93EC472C0EC219D76C5" collectingDate="2023-08-24" collectorName="Ferreira &amp; Benitez-Bosco &amp; Padilha" country="Portugal" county="Gouveia" elevation="976" latitude="40.454" location="Casais de Folgosinho" longLatPrecision="69" longitude="-7.493" municipality="Rio Mondego" specimenCount="2" specimenCount-female="1" specimenCount-male="1" stateProvince="Guarda">
<collectingCounty id="3DF7C887EB6D97B0EC843C5208842486">Gouveia</collectingCounty>
,
<collectingMunicipality id="94F7B4325A655A91AADA65E29A21C072">Rio Mondego</collectingMunicipality>
,
<location id="2138E217D1CFE8C7C37DE0BB8EFB9D8F" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:2138E217D1CFE8C7C37DE0BB8EFB9D8F" country="Portugal" county="Gouveia" latitude="40.454" longLatPrecision="69" longitude="-7.493" municipality="Rio Mondego" name="Casais de Folgosinho" stateProvince="Guarda">
Casais
<location id="20FC892DF6AB1D97E2942762DCC5ECF7" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:20FC892DF6AB1D97E2942762DCC5ECF7" country="Portugal" county="Gouveia" latitude="40.454" longLatPrecision="69" longitude="-7.493" municipality="Rio Mondego" name="de Folgosinho" stateProvince="Guarda">de Folgosinho</location>
</location>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0E2GBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.493000,40.454000]}">
<geoCoordinate id="B578696093711574E0D07EAF24D5EB8D" degrees="40.454" direction="north" orientation="latitude" precision="55" value="40.454">40.454 ° N</geoCoordinate>
,
<geoCoordinate id="99ED2B3137DDA30F9F52BFFCB1B00AC2" degrees="7.493" direction="west" orientation="longitude" precision="55" value="-7.493">7.493 ° W</geoCoordinate>
</named-content>
,
<elevation id="E56175ACE9E1DE12BFF2DA8A84732F51" metricMagnitude="2" metricUnit="m" metricValue="9.76" unit="m" value="976.0">
<quantity id="E7045364EE087A8AC8ECE9FA711F1D97" metricMagnitude="2" metricUnit="m" metricValue="9.76" unit="m" value="976.0">976 m</quantity>
a. s. l.
</elevation>
,
<date id="A7CB70063ED27FB1CFB2184B52EC3E4C" value="2023-08-24">
<collectingDate id="D79EC2B239E4F82E83739B862A6C79DA" value="2023-08-24">24 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="54A69086E87D4885E74C8355E08D7B4E">Ferreira</collectorName>
,
<collectorName id="B995488A3BDCC234EA4952E299761B7C">Benitez-Bosco</collectorName>
&amp;
<collectorName id="759FCAB238CE8792F0193D6D005D2BFE">Padilha</collectorName>
,
<specimenCount id="D784C973B15820FFDA87BAAAA57E2C80" count="1" type="male">1 ♂</specimenCount>
,
<specimenCount id="CC5BBE54FB21C53D8EDC8EE05248D734" count="1" type="female">1 ♀</specimenCount>
(sequenced)
</materialsCitation>
; •
<materialsCitation id="1164093108857C67F97D7214A18CBD04" collectingDate="2023-08-22" collectorName="Ferreira &amp; Benitez-Bosco &amp; Padilha" country="Portugal" elevation="734" latitude="40.399" location="Poio do Leao" longLatPrecision="69" longitude="-7.541" municipality="Manteigas" specimenCount="1" specimenCount-male="1" stateProvince="Guarda">
<collectingMunicipality id="95A65F935955C06C8BB57A7A10BCDA1E">Manteigas</collectingMunicipality>
,
<location id="793E3773180654940B01C2E80185141A" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:793E3773180654940B01C2E80185141A" country="Portugal" latitude="40.399" longLatPrecision="69" longitude="-7.541" municipality="Manteigas" name="Poio do Leao" stateProvince="Guarda">Poio do Leão</location>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0EDHBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.541000,40.399000]}">
<geoCoordinate id="A00B79C70105ED978A992E645A37EBFD" degrees="40.399" direction="north" orientation="latitude" precision="55" value="40.399">40.399 ° N</geoCoordinate>
,
<geoCoordinate id="7DD8EFD6782BE29727597C10CF5A752E" degrees="7.541" direction="west" orientation="longitude" precision="55" value="-7.541">7.541 ° W</geoCoordinate>
</named-content>
,
<elevation id="F195BBCBB65BDD8D4242FF50C2B93D89" metricMagnitude="2" metricUnit="m" metricValue="7.34" unit="m" value="734.0">
<quantity id="35165B1F95EA3288A80AAB31201839EA" metricMagnitude="2" metricUnit="m" metricValue="7.34" unit="m" value="734.0">734 m</quantity>
a. s. l.
</elevation>
,
<date id="19548B340B489E022E3BA614DD90EDED" value="2023-08-22">
<collectingDate id="716E41691785A6970A79D14107B75BAF" value="2023-08-22">22 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="6FC44261FB2683C4126336E40BF5F055">Ferreira</collectorName>
,
<collectorName id="85FA5EA82FCE6E147B4715F0B8CD50A7">Benitez-Bosco</collectorName>
&amp;
<collectorName id="B7263470A59E707882D0580FD98A5D19">Padilha</collectorName>
,
<specimenCount id="A3A6520B850E0E6D0E323FAC88CE0F3F" count="1" type="male">1 ♂</specimenCount>
(sequenced)
</materialsCitation>
; •
<materialsCitation id="715AFFB7BDD78E1275A1EF64F1FE62FE" collectingDate="2023-08-22" collectorName="Ferreira &amp; Benitez-Bosco &amp; Padilha &amp; Andrade &amp; Stur" country="Portugal" county="Manteigas" elevation="672" latitude="40.403" location="Ponte" longLatPrecision="69" longitude="-7.526" municipality="Zezere" specimenCount="1" specimenCount-male="1" stateProvince="Guarda">
<collectingCounty id="763937795D5DB9F1DACA53656BAEA031">Manteigas</collectingCounty>
,
<collectingMunicipality id="3598D08FEF9C3E1AB9B499134B8D408D">Zêzere</collectingMunicipality>
,
<location id="6724C42DA287FB3FFA49BE2BE0602CD8" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:6724C42DA287FB3FFA49BE2BE0602CD8" country="Portugal" county="Manteigas" latitude="40.403" longLatPrecision="69" longitude="-7.526" municipality="Zezere" name="Ponte" stateProvince="Guarda">Ponte</location>
dos
<location id="A1D4746CA114227F09087569EC80C89A" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:A1D4746CA114227F09087569EC80C89A" country="Portugal" county="Manteigas" latitude="40.403" longLatPrecision="69" longitude="-7.526" municipality="Zezere" name="Frades" stateProvince="Guarda">Frades</location>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0ELHBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.526000,40.403000]}">
<geoCoordinate id="FA585FE4EC41FFC7F7800064154A0184" degrees="40.403" direction="north" orientation="latitude" precision="55" value="40.403">40.403 ° N</geoCoordinate>
,
<geoCoordinate id="D1E7191FB5B592BCF3FF0ECF70A7FD9E" degrees="7.526" direction="west" orientation="longitude" precision="55" value="-7.526">7.526 ° W</geoCoordinate>
</named-content>
,
<elevation id="DD44648A33B95DED4E54409AC9B2F506" metricMagnitude="2" metricUnit="m" metricValue="6.72" unit="m" value="672.0">
<quantity id="EA2A66A3CD9E14A76F899E2AC3A930BE" metricMagnitude="2" metricUnit="m" metricValue="6.72" unit="m" value="672.0">672 m</quantity>
a. s. l.
</elevation>
,
<date id="7ED628C440F15DCB8960CA70CB2184CC" value="2023-08-22">
<collectingDate id="411827F18B5F8BDAE42078D70B54DF7C" value="2023-08-22">22 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="D9CE6A3DA1BE8C64552B8DB288593949">Ferreira</collectorName>
,
<collectorName id="06E3248E42F790AE770B82AD695C27B5">Benitez-Bosco</collectorName>
,
<collectorName id="272A3A7655319E408082B966DE0C364E">Padilha</collectorName>
,
<collectorName id="EDDF2E68E0D344C9C6809EBED588C43A">Andrade</collectorName>
&amp;
<collectorName id="F03CD084DC124F831587F85F7CB35BDF">Stur</collectorName>
<specimenCount id="CE46E02C1B2FFACFDC12FC55AD8FF943" count="1" type="male">1 ♂</specimenCount>
(sequenced)
</materialsCitation>
; •
<materialsCitation id="DC82761A0E1A325223FBCA6BE775E222" collectingDate="2023-08-23" collectorName="Ferreira &amp; Benitez-Bosco &amp; Padilha" country="Portugal" county="Seia" elevation="555" latitude="40.385" location="Central" longLatPrecision="69" longitude="-7.706" municipality="Rio Alva" specimenCount="1" specimenCount-female="1" stateProvince="Guarda">
<collectingCounty id="59CF4268F128F017E0EFD32383E2AE0A">Seia</collectingCounty>
,
<collectingMunicipality id="B842F53F855A3C7B97420BDC09F8ADB6">Rio Alva</collectingMunicipality>
,
<location id="F331AAFCA89E7F066647BA9A65E85DEB" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:F331AAFCA89E7F066647BA9A65E85DEB" country="Portugal" county="Seia" latitude="40.385" longLatPrecision="69" longitude="-7.706" municipality="Rio Alva" name="Central" stateProvince="Guarda">Central</location>
hidroelétrica de
<location id="511029A77512724F9217D4A840D108EB" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:511029A77512724F9217D4A840D108EB" country="Portugal" county="Seia" latitude="40.385" longLatPrecision="69" longitude="-7.706" municipality="Rio Alva" name="Ponte" stateProvince="Guarda">Ponte</location>
dos
<location id="049DD8DCFA6646DF11B4B75CD1692B7B" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:049DD8DCFA6646DF11B4B75CD1692B7B" country="Portugal" county="Seia" latitude="40.385" longLatPrecision="69" longitude="-7.706" municipality="Rio Alva" name="Jugais" stateProvince="Guarda">Jugais</location>
, river,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0ETHBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.706000,40.385000]}">
<geoCoordinate id="01B2977EA4F067C75E4403A38C2102BD" degrees="40.385" direction="north" orientation="latitude" precision="55" value="40.385">40.385 ° N</geoCoordinate>
,
<geoCoordinate id="FA47FCFE122865090E315C646EFADB16" degrees="7.706" direction="west" orientation="longitude" precision="55" value="-7.706">7.706 ° W</geoCoordinate>
</named-content>
,
<elevation id="E1ED0C1EF14867BBEE46B63D280755FB" metricMagnitude="2" metricUnit="m" metricValue="5.55" unit="m" value="555.0">
<quantity id="B86016E7AB57489CBD60ABC6B3A9392D" metricMagnitude="2" metricUnit="m" metricValue="5.55" unit="m" value="555.0">555 m</quantity>
a. s. l.
</elevation>
,
<date id="16D2384EF60F35211A0C5805604C91FD" value="2023-08-23">
<collectingDate id="DB730227AC373BCC90116C5B48D5CE3C" value="2023-08-23">23 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="3F142B12240801ADD116473981C15175">Ferreira</collectorName>
,
<collectorName id="FC158522670B332FA2D66C4C8AECF476">Benitez-Bosco</collectorName>
&amp;
<collectorName id="982D04C5655324989A6EA616FC227EEE">Padilha</collectorName>
,
<specimenCount id="BA9A23BB8C6ACE50C10C867F4B7561AD" count="1" type="female">1 ♀</specimenCount>
(sequenced)
</materialsCitation>
; •
<materialsCitation id="3074CA28EF92A61D8E48AB4AD9C69F71" collectingDate="2023-08-23" collectorName="Ferreira &amp; Benitez-Bosco &amp; Padilha" country="Portugal" elevation="791" latitude="40.395" location="Nossa Senhora do Desterro" longLatPrecision="69" longitude="-7.694" municipality="Rio Alva" specimenCount="1" specimenCount-female="1" stateProvince="Braganca">
<collectingMunicipality id="EE0A8C78538A680660A600FBBBE4A4FA">Rio Alva</collectingMunicipality>
,
<location id="5B7322DF6946B02266270101499AEC28" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:5B7322DF6946B02266270101499AEC28" country="Portugal" latitude="40.395" longLatPrecision="69" longitude="-7.694" municipality="Rio Alva" name="Nossa Senhora do Desterro" stateProvince="Braganca">Nossa Senhora do Desterro</location>
, river,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0E2HBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.694000,40.395000]}">
<geoCoordinate id="C84BAB7A911066D21E19ADD6E455BE50" degrees="40.395" direction="north" orientation="latitude" precision="55" value="40.395">40.395 ° N</geoCoordinate>
,
<geoCoordinate id="87F84E132AC251C439735F57B0124801" degrees="7.694" direction="west" orientation="longitude" precision="55" value="-7.694">7.694 ° W</geoCoordinate>
</named-content>
,
<elevation id="E7D9DE5860919562A0FB065330B501DE" metricMagnitude="2" metricUnit="m" metricValue="7.909999999999999" unit="m" value="791.0">
<quantity id="C86B6EAFD29F598AB6AB1D5C8A99C200" metricMagnitude="2" metricUnit="m" metricValue="7.909999999999999" unit="m" value="791.0">791 m</quantity>
a. s. l.
</elevation>
,
<date id="859A051C78CF80A61A8D9985FC3F784C" value="2023-08-23">
<collectingDate id="0D21D3F9B8AABCA8C434349C1BFEC1CD" value="2023-08-23">23 Aug. 2023</collectingDate>
</date>
, leg.
<collectorName id="B86954D336FCE190DDE00B9A8C14B505">Ferreira</collectorName>
,
<collectorName id="03B127CA7D1AF9CC34FABC11F1D6FC57">Benitez-Bosco</collectorName>
&amp;
<collectorName id="363B244C0BAB4E639370267E44144A97">Padilha</collectorName>
,
<specimenCount id="7AE864621CE89E76DC874DFCCB9EF84A" count="1" type="female">1 ♀</specimenCount>
(sequenced).
<emphasis id="59895D963B1A4EF2BF563D63A7CFBE52" bold="true">
<collectingRegion id="2ECBB5D4621213ACA3E6BC6DD2E19917" country="Portugal" name="Braganca">Bragança</collectingRegion>
</emphasis>
</materialsCitation>
: •
<materialsCitation id="1195BE168630D006F0C78443AB9C1112" collectingDate="2023-07-13" collectorName="Ferreira &amp; Padilha" country="Portugal" elevation="256" latitude="41.665" location="Torre de Dona Chama" longLatPrecision="69" longitude="-7.146" municipality="Mirandela" specimenCount="1" specimenCount-female="1" stateProvince="Vila Real">
<collectingMunicipality id="E800099815BDA4499CC61CD7D5540FCA">Mirandela</collectingMunicipality>
,
<location id="CD4EFD8BBD08A6EE603F56FEC015823D" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:CD4EFD8BBD08A6EE603F56FEC015823D" country="Portugal" latitude="41.665" longLatPrecision="69" longitude="-7.146" municipality="Mirandela" name="Torre de Dona Chama" stateProvince="Vila Real">Torre de Dona Chama</location>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0EFIBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.146000,41.665000]}">
<geoCoordinate id="6C62B37DB82380237FCD5587959B0DBA" degrees="41.665" direction="north" orientation="latitude" precision="55" value="41.665">41.665 ° N</geoCoordinate>
,
<geoCoordinate id="ACFD93A93425884671F6753E069D1982" degrees="7.146" direction="west" orientation="longitude" precision="55" value="-7.146">7.146 ° W</geoCoordinate>
</named-content>
,
<elevation id="356D2C44F719D48016FE1856BDB841E9" metricMagnitude="2" metricUnit="m" metricValue="2.56" unit="m" value="256.0">
<quantity id="64C80E9C13588429008199EDD77DAF00" metricMagnitude="2" metricUnit="m" metricValue="2.56" unit="m" value="256.0">256 m</quantity>
a. s. l.
</elevation>
,
<date id="6A0CD65215ADE15FB79B18B22256D868" value="2023-07-13">
<collectingDate id="EE87672355A9D6E2EC28E4D8F0D3E40C" value="2023-07-13">13 Jul. 2023</collectingDate>
</date>
, leg.
<collectorName id="B88B60724B0B239017867C3681C45779">Ferreira</collectorName>
&amp;
<collectorName id="85A4F83713AD5ADFA741898A5A319C32">Padilha</collectorName>
,
<specimenCount id="7FD762B61C09DC1BA5E7F656496EE210" count="1" type="female">1 ♀</specimenCount>
(sequenced).
<emphasis id="B448402DEDCE1B60BA3467D8F9BD4007" bold="true">
<collectingRegion id="BD8B78E1DA3C0C6A067C52154DB4E7E8" country="Portugal" name="Vila Real">Vila Real</collectingRegion>
</emphasis>
</materialsCitation>
<materialsCitation id="A15C5EC758E52D1601C8F20F4F455968" collectingDate="2023-07-12" collectorName="Ferreira &amp; Padilha" country="Portugal" elevation="421" latitude="41.409" location="Noura" longLatPrecision="69" longitude="-7.417" municipality="Murca" specimenCount="1" specimenCount-male="1" stateProvince="Vila Real">
<collectingMunicipality id="27C1ECC2CF713D13B092C1397B0FE030">Murça</collectingMunicipality>
,
<location id="14FCBEF1C307124FA6A2C2C881469756" LSID="urn:lsid:plazi:treatment:B9105C9F1D025007A1F4C88F5C49A642:14FCBEF1C307124FA6A2C2C881469756" country="Portugal" latitude="41.409" longLatPrecision="69" longitude="-7.417" municipality="Murca" name="Noura" stateProvince="Vila Real">Noura</location>
stream,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0EPIBM" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[-7.417000,41.409000]}">
<geoCoordinate id="0EB9FAEC0467B030CF97CF2D2C1C7D61" degrees="41.409" direction="north" orientation="latitude" precision="55" value="41.409">41.409 ° N</geoCoordinate>
,
<geoCoordinate id="9CA463FA4DE2F4164D47BB1B5FC7A50D" degrees="7.417" direction="west" orientation="longitude" precision="55" value="-7.417">7.417 ° W</geoCoordinate>
</named-content>
,
<elevation id="C74045BF47D7E2DEDB1A36053D853129" metricMagnitude="2" metricUnit="m" metricValue="4.21" unit="m" value="421.0">
<quantity id="B3D75DA58C405A1EA39ACAA3EA96843F" metricMagnitude="2" metricUnit="m" metricValue="4.21" unit="m" value="421.0">421 m</quantity>
a. s. l.
</elevation>
,
<date id="96BB6C0BF84A4F086B455E256A5563D2" value="2023-07-12">
<collectingDate id="686D88D79B483C915E0E9A8D26AED7A9" value="2023-07-12">12 Jul. 2023</collectingDate>
</date>
leg.
<collectorName id="D7DC28F0E7E4775B149F97DB7BC503F7">Ferreira</collectorName>
&amp;
<collectorName id="18B2191687E48DE07E951A74EF0AEA6A">Padilha</collectorName>
,
<specimenCount id="F4592A350BC6318DE5DADFA0B3BD68FB" count="1" type="male">1 ♂</specimenCount>
(sequenced)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="SECID0EUIBM" type="remarks">
<paragraph id="D96117BDD5C151B0CE9A231A3CEE21B5">
<heading id="A9F87EFD57B74F6CA60FF64504293597" reason="title">Remarks.</heading>
</paragraph>
<paragraph id="069A08EB9BEE4DEBE8599B885A9C49B4">
In this study, specimens keying to
<taxonomicName id="BA5F1C4C7113B5BD91A5EC14942903DE" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="771BB68E15EED4E9A78C54F49EAFB265" italics="true">H. longiporus</emphasis>
</taxonomicName>
in
<bibRefCitation id="DF6DD351DDE7E65AE33A3BF1AF510A02" DOI="10.1007/978-3-8274-2689-5" author="Gerecke R &amp; Gledhill T &amp; Pešić V &amp; Smit H" editor="Gerecke R" etAl="et al." firstAuthor="Gerecke" journalOrPublisher="7 / 2-3. Springer-Verlag Berlin, Heidelberg" pagination="1-429" refId="B16" refString="Gerecke R, Gledhill T, Pešić V, Smit H (2016) Chelicerata: Acari III. In: Gerecke R (Ed.) Süßwasserfauna von Mitteleuropa, Bd. 7 / 2-3. Springer-Verlag Berlin, Heidelberg, 1429. https://doi.org/10.1007/978-3-8274-2689-5" title="Chelicerata: Acari III." volumeTitle="Süßwasserfauna von Mitteleuropa, Bd." year="2016">Gerecke et al. (2016)</bibRefCitation>
have DNA barcodes that cluster in three unique BINs (
<ext-link id="A91FCEA06A9A0C34E49EBF66B662BDD9" ext-link-type="uri" xlink_href="http://boldsystems.org/index.php/Public_BarcodeCluster?clusteruri=BOLD:AFV9997" xlink_type="simple">
<collectionCode id="C81A6CE226C458ED63497778C71A296A">BOLD</collectionCode>
: AFV 9997
</ext-link>
,
<ext-link id="1CFD323EBD64A19C67C278E64689790E" ext-link-type="uri" xlink_href="http://boldsystems.org/index.php/Public_BarcodeCluster?clusteruri=BOLD:AFW1423" xlink_type="simple">
<collectionCode id="126AA0B35AF766C8824E69CB90F46A66">BOLD</collectionCode>
: AFW 1423
</ext-link>
,
<ext-link id="05A3D63329EAA23E73319E4D6048B7F4" ext-link-type="uri" xlink_href="http://boldsystems.org/index.php/Public_BarcodeCluster?clusteruri=BOLD:AFV9998" xlink_type="simple">
<collectionCode id="F3E0413FFAEE0D71F80C1DFE8A420F03">BOLD</collectionCode>
: AFV 9998
</ext-link>
) (Fig.
<figureCitation id="651A2E83F7F38EAD3D4B17503587AFE9" captionStart="Figure 7" captionStartId="F7" captionText="Figure 7. Neighbor-Joining tree of the Hygrobates longiporus complex obtained from 53 nucleotide COI sequences; 23 sequences were taken from Pešić et al. (2022 a, 2023 f), 26 sequences from Portugal are newly generated in this study, and four private sequences (HYDIR 005-23, HYDIR 006-23, HYDIR 019-23, HYDIR 020-23) from Turkey were directly taken from the BOLD. Hygrobates balcanicus from Portugal was used as outgroup. BINs are based on the barcode analysis from 16 May 2024. Country codes: FR = France, GR = Greece, IR = Iran, NO = Norway, PT = Portugal, RS = Serbia, TR = Turkey. Bootstrap values &gt; 50 % from 1000 bootstrap replicates on branches." figureDoi="10.3897/zookeys.1217.131730.figure7" httpUri="https://binary.pensoft.net/fig/1164871">7</figureCitation>
). The
<emphasis id="45DF9FFB8E6DEEBF3C9A272C9B0D75C3" italics="true">p</emphasis>
- distance to the nearest neigbour ranged between 11.3813.62 %, far exceeding the species thresholds (6.08 %
<abbrev id="ABBRID0E5JBM" xlink_title="Kimura 2-parameter model">K 2 P</abbrev>
distance) obtained in the study on the
<taxonomicName id="F08F66A111D56E4BF88D98CF84357387" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="5D5BEBB78A617DC3B0A3015C869A6245" italics="true">H. longiporus</emphasis>
</taxonomicName>
complex by
<bibRefCitation id="90286400B77F765C15AE8610A9AC74E6" DOI="10.11158/saa.27.7.7" author="Pešić V &amp; Esen Y &amp; Gülle P &amp; Zawal A &amp; Saboori A &amp; Jovanović M &amp; Bańkowska A &amp; Smit H" issue="7" journalOrPublisher="Systematic &amp; Applied Acarology" pagination="1393-1407" refId="B36" refString="Pešić V, Esen Y, Gülle P, Zawal A, Saboori A, Jovanović M, Bańkowska A, Smit H (2022 a) New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia). Systematic &amp; Applied Acarology 27 (7): 13931407. https://doi.org/10.11158/saa.27.7.7" title="New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia)." volume="27">Pešić et al. (2022 a</bibRefCitation>
).
</paragraph>
<caption id="78DCCC4BA05FA150F23F3F479FB72244" ID-DOI="10.3897/zookeys.1217.131730.figure7" ID-arpha="35463114-B2BF-57B2-AD29-2281BC7A2A41" httpUri="https://binary.pensoft.net/fig/1164871" startId="F7">
<paragraph id="ED92412C30ED53C02A970DF5C5140A39">
<label id="05C1B9F7A4A7F4D0C7871BA8960E1C9F">Figure 7.</label>
</paragraph>
<paragraph id="5E228144560E1EF123C811A46A5E0CDE">
Neighbor-Joining tree of the
<taxonomicName id="800D1891C5FB2797494E4A55AAA2B6BF" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="A6234CACF2154517FA20B6F89B2453B5" italics="true">Hygrobates longiporus</emphasis>
</taxonomicName>
complex obtained from 53 nucleotide
<abbrev id="ABBRID0EELBM" xlink_title="mitochondrial cytochrome c oxidase subunit I">COI</abbrev>
sequences; 23 sequences were taken from
<bibRefCitation id="07CBAEF00D13F75891C1027021A18065" DOI="10.11158/saa.27.7.7" author="Pešić V &amp; Esen Y &amp; Gülle P &amp; Zawal A &amp; Saboori A &amp; Jovanović M &amp; Bańkowska A &amp; Smit H" issue="7" journalOrPublisher="Systematic &amp; Applied Acarology" pagination="1393-1407" refId="B36" refString="Pešić V, Esen Y, Gülle P, Zawal A, Saboori A, Jovanović M, Bańkowska A, Smit H (2022 a) New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia). Systematic &amp; Applied Acarology 27 (7): 13931407. https://doi.org/10.11158/saa.27.7.7" title="New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia)." volume="27">Pešić et al. (2022 a</bibRefCitation>
,
<bibRefCitation id="22251A9AAE2E06B5F2C3CE2892BF57CC" DOI="10.11158/saa.28.4.10" author="Pešić V &amp; Esen E &amp; Gülle P &amp; Zawal A &amp; Jovanović M &amp; Rewicz T &amp; Smit H" issue="4" journalOrPublisher="Systematic &amp; Applied Acarology" pagination="742-754" refId="B43" refString="Pešić V, Esen E, Gülle P, Zawal A, Jovanović M, Rewicz T, Smit H (2023 f) An appraisal of the water mite genus Hygrobates Koch, 1837 from Türkiye, based on morphological and molecular data (Acariformes, Hydrachnidia, Hygrobatidae). Systematic &amp; Applied Acarology 28 (4): 742754. https://doi.org/10.11158/saa.28.4.10" title="An appraisal of the water mite genus Hygrobates Koch, 1837 from Türkiye, based on morphological and molecular data (Acariformes, Hydrachnidia, Hygrobatidae)." volume="28">2023 f</bibRefCitation>
), 26 sequences from Portugal are newly generated in this study, and four private sequences (HYDIR 005-23, HYDIR 006-23, HYDIR 019-23, HYDIR 020-23) from Turkey were directly taken from the
<abbrev id="ABBRID0EQLBM" xlink_title="Barcode of Life Data Systems">BOLD</abbrev>
.
<taxonomicName id="403A4014ACFAD2CEEA16D4E71F23BAFB" authorityName="Pesic" authorityYear="2020" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="balcanicus">
<emphasis id="792D47A8B64BAD749C94521C278F1871" italics="true">Hygrobates balcanicus</emphasis>
</taxonomicName>
from Portugal was used as outgroup. BINs are based on the barcode analysis from 16 May 2024. Country codes: FR = France, GR = Greece, IR = Iran, NO = Norway, PT = Portugal, RS = Serbia, TR = Turkey. Bootstrap values&gt; 50 % from 1000 bootstrap replicates on branches.
</paragraph>
</caption>
<paragraph id="318C556522281ED5322BA0173CB5E7E1">
The identity of
<taxonomicName id="45815C8B39B64678ECBEE7321C06C8B4" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="DE35C77E56361F31B7A1FF633AF054FE" italics="true">H. longiporus</emphasis>
</taxonomicName>
was recently questioned by
<bibRefCitation id="F02E6554625C8F4022340DAD30A7D356" DOI="10.37828/em.2021.49.2" author="Pešić V &amp; Jovanović M &amp; Manović A &amp; Karaouzas I &amp; Smit H" journalOrPublisher="Ecologica Montenegrina" pagination="20-34" refId="B35" refString="Pešić V, Jovanović M, Manović A, Karaouzas I, Smit H (2021 b) New records of water mites from the Balkans revealed by DNA barcoding (Acari, Hydrachnidia). Ecologica Montenegrina 49: 2034. https://doi.org/10.37828/em.2021.49.2" title="New records of water mites from the Balkans revealed by DNA barcoding (Acari, Hydrachnidia)." volume="49">Pešić et al. (2021 b</bibRefCitation>
,
<bibRefCitation id="6DE03925EA9D19C2163BA54B617D3CD5" DOI="10.11158/saa.27.7.7" author="Pešić V &amp; Esen Y &amp; Gülle P &amp; Zawal A &amp; Saboori A &amp; Jovanović M &amp; Bańkowska A &amp; Smit H" issue="7" journalOrPublisher="Systematic &amp; Applied Acarology" pagination="1393-1407" refId="B36" refString="Pešić V, Esen Y, Gülle P, Zawal A, Saboori A, Jovanović M, Bańkowska A, Smit H (2022 a) New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia). Systematic &amp; Applied Acarology 27 (7): 13931407. https://doi.org/10.11158/saa.27.7.7" title="New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia)." volume="27">2022 a</bibRefCitation>
) who found that DNA barcodes of the specimens assigned to the latter species in Europe and
<collectingCountry id="CF7488FB24EEC8B328347F42902BB5AB" name="Turkey">Turkey</collectingCountry>
cluster within four distinct genetic lineages. The first cluster (
<ext-link id="D5941174A011B14BD66375241F96DA8E" ext-link-type="uri" xlink_href="http://boldsystems.org/index.php/Public_BarcodeCluster?clusteruri=BOLD:AEB8359" xlink_type="simple">
<collectionCode id="38D7E63040BCAF93071FBD676878010E">BOLD</collectionCode>
: AEB 8359
</ext-link>
) comprises a large number of
<taxonomicName id="622A23DF9DC31B71B9970C5199E16DD8" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="F099727FE6AFE2BB4875F36FF7CDD8AC" italics="true">longiporus</emphasis>
</taxonomicName>
- like specimens from different parts of Europe, including
<collectingCountry id="4AC7C9442FD0B6A97439990749ED2BCB" name="Norway">Norway</collectingCountry>
, from where the species was originally described (
<bibRefCitation id="DDA49B94BB48544AE5BA0946839B66B3" author="Thor" firstAuthor="Thor" journalOrPublisher="Archiv for Mathematik og Naturvidenskab" pagination="1-12" refId="B48" refString="Thor S (1898) Nye hydrachnideformer, fundne i Norge sommeren 1898, Forelobig meddelelse. Archiv for Mathematik og Naturvidenskab 20: 112." title="Nye hydrachnideformer, fundne i Norge sommeren 1898, Forelobig meddelelse." volume="20" year="1898">Thor 1898</bibRefCitation>
). The second cluster (
<ext-link id="EE8B156E4B72D4D6CCA8EC348F85207D" ext-link-type="uri" xlink_href="http://boldsystems.org/index.php/Public_BarcodeCluster?clusteruri=BOLD:AEM7299" xlink_type="simple">
<collectionCode id="33BFDA86A42A1F827B2255583889A8D3">BOLD</collectionCode>
: AEM 7299
</ext-link>
) is restricted to SE Europe and E
<collectingCountry id="D6BD21C67BF4981BBA5FDEF4ABA01041" name="Turkey">Turkey</collectingCountry>
, while the third cluster (
<ext-link id="9AECDB1B2D3948946AB6397D1C3AB5BE" ext-link-type="uri" xlink_href="http://boldsystems.org/index.php/Public_BarcodeCluster?clusteruri=BOLD:AEO0825" xlink_type="simple">
<collectionCode id="812795714D19ACE5061EC8E96483F829">BOLD</collectionCode>
: AEO 0825
</ext-link>
) is known only from
<collectingCountry id="F01B0C8B1391B951A03A53F40F96BE19" name="France">Corsica</collectingCountry>
. Most likely the latter two clusters represent a cryptic species new to science. The fourth clade, known from northern
<collectingCountry id="8FE58DEC5DBF2E31FA7D3A83E5D28238" name="Iran">Iran</collectingCountry>
and eastern
<collectingCountry id="26ADD56323AA1CD250C60F42DEED252D" name="Turkey">Turkey</collectingCountry>
, recently was described by
<bibRefCitation id="AFE802A4B0955C89F22D633FE22D9BAA" DOI="10.11158/saa.27.7.7" author="Pešić V &amp; Esen Y &amp; Gülle P &amp; Zawal A &amp; Saboori A &amp; Jovanović M &amp; Bańkowska A &amp; Smit H" issue="7" journalOrPublisher="Systematic &amp; Applied Acarology" pagination="1393-1407" refId="B36" refString="Pešić V, Esen Y, Gülle P, Zawal A, Saboori A, Jovanović M, Bańkowska A, Smit H (2022 a) New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia). Systematic &amp; Applied Acarology 27 (7): 13931407. https://doi.org/10.11158/saa.27.7.7" title="New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia)." volume="27">Pešić et al. (2022 a</bibRefCitation>
) as
<taxonomicName id="859335EEEC2495710C0DC0E46577656F" authority="Pesic &amp; Smit, 2022" authorityName="Pesic &amp; Smit" authorityYear="2022" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="thori">
<emphasis id="3095AB54264605660EB6A9BF06A4EFD6" italics="true">H. thori</emphasis>
<bibRefCitation id="4615971EB2415D231C54EF189F15BABB" DOI="10.1080/01647954.2022.2086619" author="Pešić V &amp; Smit H" journalOrPublisher="International Journal of Acarology" pagination="418-428" refId="B29" refString="Pesic V, Smit H (2022) Water mites of Corsica: DNA barcode and morphological evidences. International Journal of Acarology 48: 418 - 428. https: // doi. org / 10.1080 / 01647954.2022. 2086619" title="Water mites of Corsica: DNA barcode and morphological evidences." volume="48" year="2022">Pešić &amp; Smit, 2022</bibRefCitation>
</taxonomicName>
.
</paragraph>
<paragraph id="9CC47B370119B7A9A5109CB7760143EE">
As emphasized by
<bibRefCitation id="E8723F49242BE0F42D9984C680FBC108" DOI="10.11158/saa.27.7.7" author="Pešić V &amp; Esen Y &amp; Gülle P &amp; Zawal A &amp; Saboori A &amp; Jovanović M &amp; Bańkowska A &amp; Smit H" issue="7" journalOrPublisher="Systematic &amp; Applied Acarology" pagination="1393-1407" refId="B36" refString="Pešić V, Esen Y, Gülle P, Zawal A, Saboori A, Jovanović M, Bańkowska A, Smit H (2022 a) New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia). Systematic &amp; Applied Acarology 27 (7): 13931407. https://doi.org/10.11158/saa.27.7.7" title="New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia)." volume="27">Pešić et al. (2022 a</bibRefCitation>
),
<bibRefCitation id="0EF667DE9E50FAEF3DE6DC67502E118C" DOI="10.11158/saa.27.7.7" author="Pešić V &amp; Esen Y &amp; Gülle P &amp; Zawal A &amp; Saboori A &amp; Jovanović M &amp; Bańkowska A &amp; Smit H" issue="7" journalOrPublisher="Systematic &amp; Applied Acarology" pagination="1393-1407" refId="B36" refString="Pešić V, Esen Y, Gülle P, Zawal A, Saboori A, Jovanović M, Bańkowska A, Smit H (2022 a) New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia). Systematic &amp; Applied Acarology 27 (7): 13931407. https://doi.org/10.11158/saa.27.7.7" title="New records of water mites from Turkey and Iran revealed by DNA barcoding, with the description of a new species (Acari, Hydrachnidia)." volume="27">a</bibRefCitation>
larger-scale study of
<taxonomicName id="A2ACCC687D60242CF4CC40C848A3B4AA" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="F88F0902D82950B35B87BA95FA7FF62D" italics="true">H. longiporus</emphasis>
</taxonomicName>
complex is needed to establish a stable taxonomy for this group. The true identity of
<taxonomicName id="FAD71255585444A11EBF5CC27369F633" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="68435EEBBC43A1BFDC10DFD0B430ADF0" italics="true">H. longiporus</emphasis>
</taxonomicName>
should be morphologically redefined with material from
<collectingCountry id="01D36E1E76B7B0A489780D92976A5FD3" name="Norway">Norway</collectingCountry>
. Morphological analysis revealed that in regard to the shape of palp and genital field, Portuguese specimens match the description of
<taxonomicName id="895EB370A42F065AD70CDB04585CE7ED" authorityName="Walter" authorityYear="1926" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="falcilaminatus">
<emphasis id="9A84E206BC267EE8362C412E0C948CCF" italics="true">H. falcilaminatus</emphasis>
</taxonomicName>
, a species originally described from Azrou,
<collectingCountry id="53403B2DF34BB2BC0219B864C011F365" name="Morocco">Morocco</collectingCountry>
on the basis of a single female (
<bibRefCitation id="008501FB2BB885159EC8A46BA7D8EC88" author="Walter" firstAuthor="Walter" issue="7-8" journalOrPublisher="Bulletin de la Société des Sciences Naturelles du Maroc" pagination="129-157" refId="B53" refString="Walter C (1926) Hydracariens du Maroc. Bulletin de la Société des Sciences Naturelles du Maroc 6 (78): 129157." title="Hydracariens du Maroc." volume="6" year="1926">Walter 1926</bibRefCitation>
) and later reported from a wide area in the western Mediterranean (
<collectingCountry id="FCE4D64C736467FB5F0A0747B831A2E5" name="Spain">Spain</collectingCountry>
and
<collectingCountry id="6196E112C9C46441AB8D3AC5BE6E6030" name="France">France</collectingCountry>
). The species was synonymized with
<taxonomicName id="3AB505F4D2414D701CAD0D4C542497F1" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="454E73C5A1CECCBEDFD6B382280C1AD8" italics="true">H. longiporus</emphasis>
</taxonomicName>
by
<bibRefCitation id="20778C00074500589845CA86076274C2" author="Lundblad O" firstAuthor="Lundblad" journalOrPublisher="Arkiv för Zoologi" pagination="1-306" refId="B25" refString="Lundblad O (1956) Zur Kenntnis süd- und mitteleuropäischer Hydrachnellen. Arkiv för Zoologi 10: 1306." title="Zur Kenntnis süd- und mitteleuropäischer Hydrachnellen." volume="10" year="1956">Lundblad (1956)</bibRefCitation>
(see also
<bibRefCitation id="8DD37CDC0CFC1016529955F81081C075" author="Gerecke" firstAuthor="Gerecke" journalOrPublisher="Lauterbornia" pagination="1-304" refId="B14" refString="Gerecke R (1991) Taxonomische, faunistische und ökologische Untersuchungen an Wassermilben (Acari, Actinedida) aus Sizilien unter Berücksichtigung anderer aquatischer Invertebraten. Lauterbornia 7: 1304." title="Taxonomische, faunistische und ökologische Untersuchungen an Wassermilben (Acari, Actinedida) aus Sizilien unter Berücksichtigung anderer aquatischer Invertebraten." volume="7" year="1991">Gerecke 1991</bibRefCitation>
for a discussion about this species). Further research, including a redescription of
<taxonomicName id="6CD6759224C15AE62ADC44CB69350A68" authorityName="Walter" authorityYear="1926" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="falcilaminatus">
<emphasis id="A9868BE4E2CAD9E4E223E31EA4F8E7CF" italics="true">H. falcilaminatus</emphasis>
</taxonomicName>
, is needed to clarify taxonomic status of the Portuguese lineages and relationship with other members of
<taxonomicName id="C94A3E1A23B337A25BA9D5632752449C" authorityName="Thor" authorityYear="1898" class="Arachnida" family="Hygrobatidae" genus="Hygrobates" kingdom="Animalia" order="Trombidiformes" phylum="Arthropoda" rank="species" species="longiporus">
<emphasis id="B413A62709287F80E7AD2FC4C8CCCF4C" italics="true">H. longiporus</emphasis>
</taxonomicName>
complex.
</paragraph>
</subSubSection>
</treatment>
</document>