247 lines
24 KiB
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247 lines
24 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/zookeys.594.5954" ID-GBIF-Dataset="a9298d9b-96c2-478a-8231-f1cd301520df" ID-PMC="PMC4926700" ID-Pensoft-Pub="1313-2970-594-73" ID-PubMed="27408559" ID-ZBK="570A4DC30CA84F7A967F3AED002FC3F4" ModsDocAuthor="" ModsDocDate="2016" ModsDocID="1313-2970-594-73" ModsDocOrigin="ZooKeys 594" ModsDocTitle="The phylogenetic position of a new species of Plakobranchus from West Papua, Indonesia (Mollusca, Opisthobranchia, Sacoglossa)" checkinTime="1464725713712" checkinUser="pensoft" docAuthor="Meyers-Munoz, Maria Angelica, van der Velde, Gerard, van der Meij, Sancia E. T., Stoffels, Bart E. M. W., van Alen, Theo, Tuti, Yosephine & Hoeksema, Bert W." docDate="2016" docId="80FA6C80194ED627FE637204448F8C86" docLanguage="en" docName="ZooKeys 594: 73-98" docOrigin="ZooKeys 594" docSource="http://dx.doi.org/10.3897/zookeys.594.5954" docTitle="Plakobranchus papua Meyers-Munoz & van der Velde, sp. n." docType="treatment" docUuid="E6FB98EC-AD98-4675-9FC4-0E205A6E3A2F" docUuidSource="ZooBank" docVersion="5" lastPageNumber="81" masterDocId="FF9DFFB6FFFA41326B2CFF9EFFF26A12" masterDocTitle="The phylogenetic position of a new species of Plakobranchus from West Papua, Indonesia (Mollusca, Opisthobranchia, Sacoglossa)" masterLastPageNumber="98" masterPageNumber="73" pageNumber="78" updateTime="1668163164565" updateUser="ExternalLinkService">
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<mods:title>The phylogenetic position of a new species of Plakobranchus from West Papua, Indonesia (Mollusca, Opisthobranchia, Sacoglossa)</mods:title>
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>Meyers-Munoz, Maria Angelica</mods:namePart>
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>van der Velde, Gerard</mods:namePart>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>van der Meij, Sancia E. T.</mods:namePart>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>Stoffels, Bart E. M. W.</mods:namePart>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>van Alen, Theo</mods:namePart>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>Tuti, Yosephine</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>Hoeksema, Bert W.</mods:namePart>
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<mods:title>ZooKeys</mods:title>
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<mods:part>
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<mods:date>2016</mods:date>
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<mods:detail type="volume">
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<mods:number>594</mods:number>
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<mods:start>73</mods:start>
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<mods:url>http://dx.doi.org/10.3897/zookeys.594.5954</mods:url>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/zookeys.594.5954</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1313-2970-594-73</mods:identifier>
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<treatment ID-GBIF-Taxon="127900652" LSID="urn:lsid:zoobank.org:act:E6FB98EC-AD98-4675-9FC4-0E205A6E3A2F" httpUri="http://treatment.plazi.org/id/80FA6C80194ED627FE637204448F8C86" lastPageId="9" lastPageNumber="81" pageId="5" pageNumber="78">
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<subSubSection pageId="5" pageNumber="78" type="multiple">
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<paragraph pageId="5" pageNumber="78">Taxon classification Animalia Sacoglossa Plakobranchidae</paragraph>
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</subSubSection>
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<subSubSection pageId="5" pageNumber="78" type="nomenclature">
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<paragraph pageId="5" pageNumber="78">
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<taxonomicName LSID="http://zoobank.org/E6FB98EC-AD98-4675-9FC4-0E205A6E3A2F" authority="Meyers-Munoz & van der Velde" class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus papua" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="papua">
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Plakobranchus papua
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<normalizedToken originalValue="Meyers-Muñoz">Meyers-Munoz</normalizedToken>
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& van der Velde
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</taxonomicName>
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<taxonomicNameLabel pageId="5" pageNumber="78">sp. n.</taxonomicNameLabel>
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Figures 2, 3, 4, 5, 6, 7a
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</paragraph>
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</subSubSection>
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<subSubSection pageId="5" pageNumber="78" type="type material">
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<paragraph pageId="5" pageNumber="78">Type material.</paragraph>
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<paragraph pageId="5" pageNumber="78">Holotype RMNH MOL.336417, length 35 mm (COI, anatomy. Genbank Accession number: KU934191). Paratype RMNH MOL.336418, length 30 mm (COI, anatomy, radula. Genbank Accession number: KU934192). Paratype RMNH MOL.336419, length 34 mm (COI, anatomy. Genbank Accession number: KU934193).</paragraph>
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<caption pageId="5" pageNumber="78">
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<paragraph pageId="5" pageNumber="78">
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Figure 3.
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus papua" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="papua">Plakobranchus papua</taxonomicName>
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. Images taken from live animals (external morphology). a Dorsal view, parapodia folded up on the dorsal body surface; arrow shows short yellow rod-like spots along the parapodial border b Dorsal view with detail of the rolled rhinophore indicated by an arrow c Detail of head, rhinophores, and pedal tentacles d Open parapodia with lamellae containing branches of the digestive gland and showing renopericardial area e Ventral view of bilobed oral prominence, narrow foot, and truncated black tail. Scale bars: a, d, e = 10 mm; b, c = 5 mm
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</paragraph>
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</caption>
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<caption pageId="5" pageNumber="78">
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<paragraph pageId="5" pageNumber="78">
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Figure 4.
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus papua" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="papua">Plakobranchus papua</taxonomicName>
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. Masticatory apparatus. a Pharynx; white arrow points to the buccal ganglion (bg)b Radula with ascus-sac (arrowed). c Detail of the ascus-sac which contain used teeth (arrowed) d Tooth, scanning electronic photograph e Tooth, light microscopy photograph f Detail of the denticles (SEM) g Row of teeth (LM). Scale bars: a, c = 50
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<normalizedToken originalValue="μm">μm</normalizedToken>
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; b = 25
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<normalizedToken originalValue="μm">μm</normalizedToken>
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;
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<normalizedToken originalValue="d–e">d-e</normalizedToken>
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= 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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; f = 5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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; g = 15
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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</paragraph>
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</caption>
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<caption pageId="5" pageNumber="78">
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<paragraph pageId="5" pageNumber="78">
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Figure 5.
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus papua" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="papua">Plakobranchus papua</taxonomicName>
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. Schematic drawing of reproductive system (terminology after
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<bibRefCitation author="Jensen, K" journalOrPublisher="Journal of Molluscan Studies" pageId="13" pageNumber="86" pagination="257 - 296" title="Anatomy of some Indo-Pacific Elysiidae (Opisthobranchia: Sacoglossa (= Ascoglossa )), with a discussion of the generic division and phylogeny." url="10.1093/mollus/58.3.257" volume="58" year="1992">Jensen 1992</bibRefCitation>
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). Scale bar: 250
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<normalizedToken originalValue="μm">μm</normalizedToken>
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. Abbreviations: ag = albumen gland, f = follicles, gr = genital receptacle, gv = gametolytic vesicle ('bursa
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<normalizedToken originalValue="copulatrix’">copulatrix'</normalizedToken>
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), m = mucus gland, p = penial bulb, pr = prostate, s = penial stylet, vd = vas deferens.
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</paragraph>
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</caption>
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<caption pageId="5" pageNumber="78">
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<paragraph pageId="5" pageNumber="78">
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Figure 6.
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus papua" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="papua">Plakobranchus papua</taxonomicName>
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. a Muscular penial bulb and stylet b Detail of the opening of the stylet. Scale bars: 100
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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</paragraph>
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</caption>
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<caption pageId="5" pageNumber="78">
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<paragraph pageId="5" pageNumber="78">
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Figure 7. a Dorsal view of a preserved
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus papua" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="papua">Plakobranchus papua</taxonomicName>
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showing white dots surrounded by a yellow outline. b Preserved
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus ocellatus" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="ocellatus">Plakobranchus ocellatus</taxonomicName>
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, ocellated spots with dark pigment surrounded by a white ring, some with a black ring (arrowed). c
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus ocellatus" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="ocellatus">Plakobranchus ocellatus</taxonomicName>
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, dorsal view of a live animal (Cebu, Philippines, 1999, photo BWH).
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus ocellatus" pageId="5" pageNumber="78" phylum="Mollusca" rank="species" species="ocellatus">Plakobranchus ocellatus</taxonomicName>
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, (d) dorsal view and (e) ventral view of preserved animal. Scale bars: a, b = 5 mm,
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<normalizedToken originalValue="c–e">c-e</normalizedToken>
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= 10 mm.
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="5" pageNumber="78" type="materials_examined">
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<paragraph pageId="5" pageNumber="78">Additional material.</paragraph>
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<paragraph pageId="5" pageNumber="78">
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RMNH MOL.336420, length 28 mm (anatomy, radula). RMNH MOL.336421, length 25 mm (anatomy, radula, penial bulb). RMNH MOL.5010422-5010434, slides of a single specimen, length 30 mm, northeast of Pulau Mansuar (
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<geoCoordinate direction="south" orientation="latitude" precision="1" value="-0.56805">S00°34.083'</geoCoordinate>
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, E130°38.525), Expedition Sta. RAJ.38, 30 November 2007 (histology). RMNH MOL.336423 (n = 7), length 15-26 mm, east side of Pulau Kri, Sorido Resort lagoon, near Jetty (
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<geoCoordinate direction="south" orientation="latitude" precision="1" value="-0.55578333">S00°33.347'</geoCoordinate>
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, E130°41.225), Expedition Sta. RAJ.02, 4 December 2007 (anatomy, penial bulb). RMNH MOL.336424, length 41 mm, south side of Pulau Kri (
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<geoCoordinate direction="south" orientation="latitude" precision="1" value="-0.5589333">S00°33.536'</geoCoordinate>
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,
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<geoCoordinate direction="east" orientation="longitude" precision="1" value="130.68764">E130°41.258'</geoCoordinate>
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), Expedition Sta. RAJ.03, 4 December 2007 (anatomy, penial bulb). RMNH MOL.336425 (n = 6), length 15-26 mm, west side of Pulau Yeben Kecil (
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<geoCoordinate direction="south" orientation="latitude" precision="1" value="-0.48906666">S00°29.344'</geoCoordinate>
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,
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<geoCoordinate direction="east" orientation="longitude" precision="1" value="130.50134">E130°30.081'</geoCoordinate>
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), Expedition Sta. RAJ.48, 6 December 2007 (anatomy, radula).
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</paragraph>
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</subSubSection>
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<subSubSection pageId="5" pageNumber="78" type="type locality">
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<paragraph pageId="5" pageNumber="78">Type locality.</paragraph>
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<paragraph pageId="5" pageNumber="78">Mangrove Creek, south Gam Island (0°30'403"S, 130°38'986"E), West Papua, Indonesia, 25 November 2007.</paragraph>
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</subSubSection>
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<subSubSection pageId="5" pageNumber="78" type="habitat">
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<paragraph pageId="5" pageNumber="78">Habitat.</paragraph>
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<paragraph pageId="5" pageNumber="78">The specimens were collected in sea grass beds and on coral reef sand between 1 and 10 m depth.</paragraph>
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</subSubSection>
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<subSubSection pageId="5" pageNumber="78" type="etymology">
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<paragraph pageId="5" pageNumber="78">Etymology.</paragraph>
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<paragraph pageId="5" pageNumber="78">The specific name papua of this species is based on the name of the Indonesian part of New Guinea (provinces Papua and West Papua) where the type material was collected.</paragraph>
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</subSubSection>
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<subSubSection lastPageId="9" lastPageNumber="82" pageId="5" pageNumber="78" type="description">
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<paragraph pageId="5" pageNumber="78">Description.</paragraph>
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<paragraph lastPageId="6" lastPageNumber="79" pageId="5" pageNumber="78">
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External morphology (Figure 3). Body elongated, dorsoventrally flattened with wide parapodial flaps folding along the dorsal midline (Figure 3a). Rhinophores extended from lateral edges of the head long, smooth, rolled (Figure 3b).
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<pageBreakToken pageId="6" pageNumber="79" start="start">An</pageBreakToken>
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eye pair belonging to the central nervous system (CNS) is visible on the head. Eyes situated very close to each other, in front of a prominent, elongated white spot (Figure 3c). The voluminous renopericardial prominence is short, whitish (Figure 3d), and covered by the parapodial flaps. The folded parapodia show a smooth surface from a dorsal view. The parapodia margin at the mid-line shows short yellow rod-like spots along the complete parapodial border (Figure 3a) such as those visible in '
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus ocellatus" pageId="6" pageNumber="79" phylum="Mollusca" rank="species" species="ocellatus">Plakobranchus ocellatus</taxonomicName>
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' (
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<bibRefCitation author="Waegele, H" journalOrPublisher="Molecular Biology and Evolution" pageId="16" pageNumber="89" pagination="699 - 706" title="Transcriptomic evidence that longevity of acquired plastids in the photosynthetic slugs Elysiatimida and Plakobranchusocellatus does not entail lateral transfer of algal nuclear genes." url="10.1093/molbev/msq239" volume="28" year="2011">
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<normalizedToken originalValue="Wägele">Waegele</normalizedToken>
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et al. 2011
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</bibRefCitation>
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: fig. 1a). When the parapodia are open, the edges appear as soft yellow pectinate margins (Figure 3d). Internally, the parapodia have thick parallel longitudinal lamellae. Only the mid-central lamellae connect directly to the renopericardial prominence and run parallel to each other towards the posterior side of the body. The most external anterior lamellae are shorter, connected through anastomosing lamellae with the most internal lamellae and through them connected with the renopericardial prominence (Figure 3d). These fine dorsal anastomosing lamellae can only be recognized in live and in freshly collected, non-preserved specimens. In preserved contracted specimens it is not possible to distinguish these dorsal anastomosing lamellae. The lamellae possess visible internal granulations corresponding to the digestive gland ramifications, which contain chloroplasts. The genital opening is situated on the right anterior side of the body, just in front of the anterior part of the parapodia, and immediately behind the rhinophore. The anal opening is located on the right anterior side of the pericardium. Ventrally a bilobed oral prominence (Figure 3e) with a very fine, undulating black line boarding the upper lip is present. Pedal tentacles are short (Figure 3c). The narrow foot has a smooth surface and truncated tail (Figure 3e).
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</paragraph>
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<paragraph pageId="6" pageNumber="79">Colouration (Figure 3). Intense ochre body with white spots scattered all over the dorsum, head, and flanks: closest to the head region, on the anterior side corresponding to 1/4 of the body length, the spots are largest, on 2/4 and 4/4 of the body length the dots are smaller, and on the 3/4 of the body length they are larger, but not as large as on the anterior part of the body. Spots are pale white with a thin yellow outline (Figure 7a). Rhinophores black, the outer tips purplish. Internal parapodial flaps ridges bright green to olive green in colour, owing to chloroplasts in the digestive gland. The pericardium region is hyaline white. The pedal tentacles are translucent white. The foot sole is white with a black tail.</paragraph>
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<paragraph pageId="6" pageNumber="79">
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Digestive system (Figure 4). The masticatory apparatus was studied in four preserved specimens (RMNH.MOL.336418, 336420, 336421, 336425). The pharynx is connected to the stomach through a short muscular lightly bent oesophagus. The stomach is approximately 25% smaller than the pharynx. A pair of salivary glands inserted and extended along the oesophagus, reaching the first third of the stomach. The pharynx has prominent dorsal septate muscles. It consists of a large cuticular pharyngeal cavity, the radular sac, and one large ascus, which are also embedded in cuticular material. Uniserial radula, small, approximately 0.50 to 0.75 mm in length with 15 teeth, eight in the ascending series and seven in the descending series (Figure 4b), and an ascus-sac which varies in size (Figures 4b, c). Teeth are sharp with triangular cusps and 10-14 denticles at each margin side, the teeth measuring 70-75
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(Figure 4
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<normalizedToken originalValue="d–g">d-g</normalizedToken>
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).
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</paragraph>
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<paragraph lastPageId="8" lastPageNumber="81" pageId="7" pageNumber="80">
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<pageBreakToken pageId="7" pageNumber="80" start="start">Reproductive</pageBreakToken>
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system (Figures 5-6). Occupies almost the central anterior part of the body, at nearly 1/4 of the body length. The distal part of the reproductive system is situated below the heart and directly behind the central nervous system. The penial bulb, which is only approximately 0.5 mm long (Figure 5: p), is situated below the rhinophores at the same level as the eyes. It possesses a sharp cuticular stylet, which is hollow, with an oblique orifice at the tip (Figure 6). The vas deferens (Figure 5: vd) bends over the mucus gland (Figure 5: m), and is orientated towards the proximal area. The distal part of the vas deferens, which is in direct connection with the penial bulb, is muscular and arched. Its proximal part is thin and coiled and is connected to two spherical genital vesicles (Figure 5: gv). After this intersection the vas deferens continues in a proximal
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<pageBreakToken pageId="8" pageNumber="81" start="start">direction</pageBreakToken>
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, where it appears as a short bulky extension, continuing in a thin duct attached to numerous ramifications, the follicles. Underneath this vesicle one large piriform white genital receptacle is present (Figure 5: gr), connected directly to the mucus gland. The mucus gland is large and divided in two lobes. The distal lobe is smaller, a little narrow and coiled. The prostate (Figure 5: pr) and albumen glands (Figure 5: ag) are extensively branched. The terminology used here is similar to the one in the schematic drawing of the reproductive system of
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<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus ocellatus" pageId="8" pageNumber="81" phylum="Mollusca" rank="species" species="ocellatus">Plakobranchus ocellatus</taxonomicName>
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by
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<bibRefCitation author="Jensen, K" journalOrPublisher="Journal of Molluscan Studies" pageId="13" pageNumber="86" pagination="257 - 296" title="Anatomy of some Indo-Pacific Elysiidae (Opisthobranchia: Sacoglossa (= Ascoglossa )), with a discussion of the generic division and phylogeny." url="10.1093/mollus/58.3.257" volume="58" year="1992">Jensen (1992</bibRefCitation>
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: fig. 22B).
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</paragraph>
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<paragraph pageId="8" pageNumber="81">Central nervous system. The central nervous system is located at the anterior part of the oesophagus and forms a circumoesophageal ring, consisting of a very small pair of buccal ganglia, a large pair of fused cerebro-pleural ganglia, and a pair of pedal ganglia. The CNS consists for the main part of the cerebral and pleural ganglia. The eyes of the CNS are situated very close to each other.</paragraph>
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<paragraph pageId="8" pageNumber="81">
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DNA analyses (Figure 8). The molecular phylogeny reconstruction of the genus
|
||
<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus" pageId="8" pageNumber="81" phylum="Mollusca" rank="genus">Plakobranchus</taxonomicName>
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contains all the currently available sequences on GenBank. The analyses in MrBayes (Bayesian inference) and Phyml (maximum likelihood) resulted in trees in which the specimens were assigned to the same clades. In Phyml the tree formed a polytomy, whereas the analyses in MrBayes showed a topology with highly resolved clades. Ten different clades can be distinguished, of which three are represented by single specimens. The largest clade contains 49 sequences. Our new species groups with
|
||
<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus" pageId="8" pageNumber="81" phylum="Mollusca" rank="genus">Plakobranchus</taxonomicName>
|
||
sp. 1 of
|
||
<bibRefCitation author="Krug, P" journalOrPublisher="Molecular Phylogenetics and Evolution" pageId="14" pageNumber="87" pagination="1101 - 1119" title="Integrative species delimitation in photosynthetic sea slugs reveals twenty candidate species in three nominal taxa studied for drug discovery, plastid symbiosis or biological control." url="10.1016/j.ympev.2013.07.009" volume="69" year="2013">Krug et al. (2013)</bibRefCitation>
|
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from Sulawesi, Indonesia and Panglao, Philippines. Over 580 base pairs there is a difference of 1.2% (7 bp) between our specimens and the specimens of
|
||
<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus" pageId="8" pageNumber="81" phylum="Mollusca" rank="genus">Plakobranchus</taxonomicName>
|
||
sp. 1 from
|
||
<bibRefCitation author="Krug, P" journalOrPublisher="Molecular Phylogenetics and Evolution" pageId="14" pageNumber="87" pagination="1101 - 1119" title="Integrative species delimitation in photosynthetic sea slugs reveals twenty candidate species in three nominal taxa studied for drug discovery, plastid symbiosis or biological control." url="10.1016/j.ympev.2013.07.009" volume="69" year="2013">Krug et al. (2013)</bibRefCitation>
|
||
.
|
||
</paragraph>
|
||
<caption pageId="8" pageNumber="81">
|
||
<paragraph pageId="8" pageNumber="81">
|
||
Figure 8. Phylogram of the
|
||
<taxonomicName class="Gastropoda" family="Plakobranchidae" genus="Plakobranchus" higherTaxonomySource="CoL" kingdom="Animalia" lsidName="Plakobranchus ocellatus" pageId="8" pageNumber="81" phylum="Mollusca" rank="species" species="ocellatus">Plakobranchus ocellatus</taxonomicName>
|
||
species complex, topology derived from MrBayes analysis. Support values represent Bayesian posterior probabilities / ML. Numbers refer to GenBank codes. Red stars represent nodes with Bayesian probability values> 90 and high ML values.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph lastPageId="9" lastPageNumber="82" pageId="8" pageNumber="81">
|
||
The ABGD analysis resulted in prior maximal intraspecific divergence of ca. 0.07. Values higher than the maximal intraspecific divergence resulted in 10 Molecular Operational Taxonomic Units (MOTUs) in both the recursive and initial partition. Each
|
||
<pageBreakToken pageId="9" pageNumber="82" start="start">of</pageBreakToken>
|
||
these
|
||
<normalizedToken originalValue="MOTU’s">MOTU's</normalizedToken>
|
||
corresponds to a clade in the phylogeny reconstruction (Figure 8). The three singletons (GQ996679, KC573731, KC573734) from the ABGD analysis were also retrieved as singletons in the molecular phylogeny.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |