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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.99.90117" ID-Pensoft-Pub="1314-4049-99-45" ID-Pensoft-UUID="47D9DA3E1C56567CA14356476272F738" ModsDocID="1314-4049-99-45" checkinTime="1692098463846" checkinUser="pensoft" docAuthor="Itagaki, Hiyori &amp; Hosoya, Tsuyoshi" docDate="2023" docId="8955027CB3DC5A4EADD1978A63F0D22B" docLanguage="en" docName="MycoKeys 99: 45-85" docOrigin="MycoKeys 99" docPubDate="2023-08-14" docSource="http://dx.doi.org/10.3897/mycokeys.99.90117" docTitle="Neobelonopsis didymospora Itagaki &amp; Hosoya 2023, sp. nov." docType="treatment" docVersion="1" id="47D9DA3E1C56567CA14356476272F738" lastPageNumber="45" masterDocId="47D9DA3E1C56567CA14356476272F738" masterDocTitle="A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan" masterLastPageNumber="85" masterPageNumber="45" pageNumber="45" updateTime="1692098463846" updateUser="pensoft">
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<mods:title id="50F4B6E0AC4859F846A0A9D7BA578F64">A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan</mods:title>
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<mods:name id="9DE1C49574D37620B6B9EC27105CE0EA" type="personal">
<mods:role id="EA8CFB58B243EC282DE46B2BD6F4D888">
<mods:roleTerm id="1188D29A3E0E47C03E2375149FA22CCA">Author</mods:roleTerm>
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<mods:namePart id="F53BFD8C177DBE3D635D04D640CC7F77">Itagaki, Hiyori</mods:namePart>
<mods:nameIdentifier id="98697777EE911FEEE606054A881F0DAC" type="ORCID">https://orcid.org/0000-0001-8678-0826</mods:nameIdentifier>
<mods:affiliation id="AFF3BE8C9CC76CAC87F5F2DCCADDE747">Department of Botany, National Museum of Nature and Science, 4 - 1 - 1, Amakubo, Tsukuba, Ibaraki, 305 - 0005, Japan</mods:affiliation>
<mods:nameIdentifier id="C654787BACF9E79CDA1332A2F89D1E1E" type="email">itagaki@kahaku.go.jp</mods:nameIdentifier>
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<mods:namePart id="51BEF827C450F2B08065D3FE93203320">Hosoya, Tsuyoshi</mods:namePart>
<mods:nameIdentifier id="C1B917C73D81EF9CA53BF041FFACE32E" type="ORCID">https://orcid.org/0000-0001-5360-5677</mods:nameIdentifier>
<mods:affiliation id="18FDFE8674E360CA590617BD0FE3C1D5">Department of Botany, National Museum of Nature and Science, 4 - 1 - 1, Amakubo, Tsukuba, Ibaraki, 305 - 0005, Japan</mods:affiliation>
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<mods:date id="D4EC82895133D028520A81BAA777FF91">2023</mods:date>
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<mods:number id="C52DC02033BE13BABDDD22C85CDDDCB7">2023-08-14</mods:number>
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<mods:number id="E4C217C8F1DC7C3E60FE2B6DC01B0391">99</mods:number>
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<mods:classification id="CD69393001E651FD1E7F72902F8641AD">journal article</mods:classification>
<mods:identifier id="E2529571003D36A2E15DC7AECF58E7F3" type="DOI">http://dx.doi.org/10.3897/mycokeys.99.90117</mods:identifier>
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<treatment id="8955027CB3DC5A4EADD1978A63F0D22B" LSID="urn:lsid:plazi:treatment:8955027CB3DC5A4EADD1978A63F0D22B" httpUri="http://treatment.plazi.org/id/8955027CB3DC5A4EADD1978A63F0D22B" lastPageNumber="45" pageId="0" pageNumber="45">
<subSubSection id="4A02B5B09D53639CA3ECFB56C183E4C3" pageId="0" pageNumber="45" type="nomenclature">
<paragraph id="2B8A2BBD3C9B530302CD3E71E9E833E1" pageId="0" pageNumber="45">
<taxonomicName id="34456BB780D4170736F7E69D24154716" LSID="8955027C-B3DC-5A4E-ADD1-978A63F0D22B" authority="Itagaki &amp; Hosoya" authorityName="Itagaki &amp; Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" higherTaxonomySource="treatment-meta" kingdom="Fungi" lsidName="Neobelonopsis didymospora" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="didymospora" status="sp. nov.">Neobelonopsis didymospora Itagaki &amp; Hosoya</taxonomicName>
<taxonomicNameLabel id="BE5D80D807B6FEEBB0A86A008D35732B" pageId="0" pageNumber="45">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection id="8F2824CFA3DE4E4D7A3682B09133F50B" pageId="0" pageNumber="45" type="description">
<paragraph id="FD2D484552E13093257B9E25318DA31A" pageId="0" pageNumber="45">
<figureCitation id="D37CD867DD9BF8CC68427B514F44AC01" captionStart="Figure 6" captionStartId="F6" captionText="Figure 6. Neobelonopsis didymospora (TNS-F- 13501, holotype) A fresh apothecia on decaying Bamboo culm B dried apothecia on decaying Bamboo culm C fresh apothecia on decaying unidentified wood (TNS-F- 86670) D dried apothecia on decaying unidentified wood (TNS-F- 86670) E vertical section of the apothecium (in LA) F reflective vacuoles in fresh marginal cells (in water) G vertical section at the basal apothecium (in lactic acid) H ascospores (in CB / LA) I asci with ascospores (in CB / LA) J paraphyses (in CB / LA) K fresh paraphyses with long refractive vacuoles (in water) L blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) M crozier at the base of ascus (in CB / LA) N three months old colony on PDA O clusters of conidiophores (in CB / LA) P-S conidiophores with conidia (in CB / LA). Scale bars: 1 mm (A-D); 50 μm (E); 20 μm (O), 10 μm (F-M, P-S)." figureDoi="10.3897/mycokeys.99.90117.figure6" httpUri="https://binary.pensoft.net/fig/892422" pageId="0" pageNumber="45">Figs 6</figureCitation>
<figureCitation id="3602B8D6E799BDD896E2C26A16D5875E" captionStart="Figure 13" captionStartId="F13" captionText="Figure 13. Line-drawings of the Neobelonopsis and Trichobelonium species I vertical section of the apothecium showing the marginal structure of ectal excipulum, medullary excipulum, and hymenium II asci III paraphyses IV ascospores. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure13" httpUri="https://binary.pensoft.net/fig/892429" pageId="0" pageNumber="45">, 13</figureCitation>
<figureCitation id="949D44E33AD3596B4F97395191E0D5FA" captionStart="Figure 14" captionStartId="F14" captionText="Figure 14. Asexual morph and hyphal structures of the Neobelonopsis and Trichobelonium species A conidiophores of N. acutata B monilioid hyphae of N. bicolor C conidiophores with conidia of N. cinnabarina D conidiophores with conidia of N. didymospora E conidiophores with conidia of N. microspora F conidiophores with conidia of N. multiguttata G conidiophores with conidia of N. obtusa H conidiophores with conidia of T. albobarbatum I hyphal mass with melanized appressorium-like structure of T. miscanthi. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure14" httpUri="https://binary.pensoft.net/fig/892430" pageId="0" pageNumber="45">, 14D</figureCitation>
</paragraph>
</subSubSection>
<subSubSection id="F5812AF7CA4EFE3693686110616E819F" pageId="0" pageNumber="45" type="etymology">
<paragraph id="0A8776C6DBA837CDC1E4475928FECE53" pageId="0" pageNumber="45">Etymology.</paragraph>
<paragraph id="09123B59102034CDF668F51D6C8F630D" pageId="0" pageNumber="45">Named after two-celled ascospores.</paragraph>
</subSubSection>
<subSubSection id="5509C9B73C06BE3EB16B077F9B54BABE" pageId="0" pageNumber="45" type="diagnosis">
<paragraph id="FDCF9F95C49CCEA2A7BEAB471F485DB6" pageId="0" pageNumber="45">Diagnosis.</paragraph>
<paragraph id="54327CAE5EEBD0691DE76A0150376CCB" pageId="0" pageNumber="45">
Resembles
<taxonomicName id="59D48715A1C599765385B4EA23929A91" authorityName="Itagaki &amp; Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis bicolor" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="bicolor">
<emphasis id="F0BA78B8340608D60A1D176AB6EC18DF" italics="true" pageId="0" pageNumber="45">Neobelonopsis bicolor</emphasis>
</taxonomicName>
, but distinguishable by sparse, minute guttles in living/dead ascospores, shorter asci, and wider host range including woody bamboos.
</paragraph>
</subSubSection>
<subSubSection id="5C8724666FA9C15E889C6A1F59DA9621" pageId="0" pageNumber="45" type="holotype">
<paragraph id="6377CB6B50A3B7D246982856E73D09E8" pageId="0" pageNumber="45">Holotype.</paragraph>
<paragraph id="D7EB3EC35F0060F152DCA82193391A23" pageId="0" pageNumber="45">TNS-F-13501, Yakushima Island, Kagoshima Pref., Japan, 19 October 2005, on decaying culms of woody bamboos, ex-holotype culture NBRC 115354.</paragraph>
</subSubSection>
<subSubSection id="A92645C3245DBD663CA8057AB4315B45" pageId="0" pageNumber="45" type="description">
<paragraph id="AD57EADC69CAC76401B1F4BC4AC1DF15" pageId="0" pageNumber="45">Description.</paragraph>
<paragraph id="25875D42716D9F3D76A05CF8DBDBDEA8" pageId="0" pageNumber="45">
<emphasis id="78D6C10FB7C95EE0386A7ABA4A636F73" bold="true" italics="true" pageId="0" pageNumber="45">Apothecia</emphasis>
superficial, without subiculum and scuta, 0.1-0.2 mm high, with blackish green (C100M100Y80-100K30) to black receptacle; disc 0.5-1 mm diam., white to bluish gray (C60M30-40Y20K60) when fresh, shrunk to 0.3-0.7 mm diam., cream (Y20K10) or olive (C40M40Y60-100K10) when dried. Ectal excipulum 30-50
<normalizedToken id="D5023439F649623EB90FAC8C8BADA31B" originalValue="µm">µm</normalizedToken>
thick at base, 20-25
<normalizedToken id="5F60E2459B34C5AB1CF691899A89E966" originalValue="µm">µm</normalizedToken>
thick at the upper flank to margin; cortical cells obovoid to clavate, (10-)12-15
<normalizedToken id="9A8092865DEFF5887D8E996EB968D64D" originalValue="×">x</normalizedToken>
7.5-10
<normalizedToken id="95B83E34DB665FDE918E05ABDD080638" originalValue="µm">µm</normalizedToken>
at base, becoming slender and closely packed at the upper flank to margin, containing refractive vacuoles at the protruding cells when mounted fresh in water. Medullary excipulum 25-38
<normalizedToken id="0EDEED6F1589E1EF0C46E8D9FAE5A6D3" originalValue="µm">µm</normalizedToken>
thick, frequently dichotomously branched, radially spreading toward the upper flask.
<emphasis id="32E794C5086275B3E30D7142F188A8CB" bold="true" italics="true" pageId="0" pageNumber="45">Asci</emphasis>
(50-)52-60(-65)
<normalizedToken id="3F8432EF8260789CC8C1AF0BAF6733C8" originalValue="×">x</normalizedToken>
5-7.5
<normalizedToken id="D73952A7181F6B408BB7BE2BCB2A3771" originalValue="µm">µm</normalizedToken>
, arising from croziers, with MLZ + apical pore.
<emphasis id="751947A0DF9DB5DBE00C70A9D354D1EE" bold="true" italics="true" pageId="0" pageNumber="45">Ascospores</emphasis>
10-14(-16)
<normalizedToken id="31727382040E064181F7274F3178FE47" originalValue="×">x</normalizedToken>
2.5-3.5
<normalizedToken id="55DB57A7D47AA7D4E547F0F5F0276D46" originalValue="µm">µm</normalizedToken>
, ellipsoid to fusiform, with subacute to acute extremes, frequently constrict at the septum, (0-)1-2-septate, hyaline, containing scattered small guttules.
<emphasis id="5A6707233E8FCE1FBDF9E4FDB292E7FC" bold="true" italics="true" pageId="0" pageNumber="45">Paraphyses</emphasis>
(45-)53-65
<normalizedToken id="9A3459E40002F6ACBDF0B97F7F5D5487" originalValue="×">x</normalizedToken>
2.5-3(-4)
<normalizedToken id="CCFADA38DEEB97888B50506BA608A8B4" originalValue="µm">µm</normalizedToken>
, simple, (1-)2-3-septate, containing long refractive vacuoles in the apical cells and first 2-3 lower cells.
<emphasis id="746279C5882BD9C09648733B8C407F41" bold="true" italics="true" pageId="0" pageNumber="45">Colony</emphasis>
of NBRC 115354 on PDA flat, entire, dense, cottony to felted, dark brown (C60M80Y80-100K10) to beige (C10M20Y20-40K10) at the center, becoming pale brown toward to the edge, same colors at the reverse side, without soluble pigment and crystals; aerial mycelium sparse to dense, white to beige.
<emphasis id="8E0CA07F736E53BC4E06664AA1C3D4E4" bold="true" italics="true" pageId="0" pageNumber="45">Conidiophores</emphasis>
solitary to occasionally aggregated on aerial hyphae, semi-macronematous, short, arising vertically or laterally from hyphae, pale to dark brown, smooth, thick-walled, sometimes branched 2-3 times, constricted at the septa, 2-3
<normalizedToken id="A881D6985D529BE8BA446C2995FF0732" originalValue="µm">µm</normalizedToken>
width;
<emphasis id="358F71C82BF8A2A8C5A67893B3BCEE6B" bold="true" italics="true" pageId="0" pageNumber="45">phialides</emphasis>
ampulliform, up to 15
<normalizedToken id="EE3C2868E77531503442D17A711ACA19" originalValue="µm">µm</normalizedToken>
long, 3.5
<normalizedToken id="F25AC23E5353CDE6FE5F18BA8EB3BA4E" originalValue="µm">µm</normalizedToken>
width at base, discrete or integrated, terminal or intercalary, hyaline to pale brown, thick-walled, with cylindrical to wide funnel-shape collarettes of 4.5-7.5
<normalizedToken id="8FF954F5D5653457D3BDBC9A87651F64" originalValue="×">x</normalizedToken>
3
<normalizedToken id="768FF617603E2E47E2FE450442FC8616" originalValue="µm">µm</normalizedToken>
;
<emphasis id="45A0730E02028A1AC0C08F65DE82E419" bold="true" italics="true" pageId="0" pageNumber="45">conidia</emphasis>
aseptate, subspherical to ellipsoid, abundantly aggregated in slimy head, 1.5-1.8
<normalizedToken id="814BBD2C293FC9BB5ABC305302DD1776" originalValue="µm">µm</normalizedToken>
diam., hyaline, thin-walled.
</paragraph>
<caption id="4651357CD751EA1CA6491C71EC768523" doi="10.3897/mycokeys.99.90117.figure6" httpUri="https://binary.pensoft.net/fig/892422" pageId="0" pageNumber="45" start="Figure 6" startId="F6">
<paragraph id="E7BDD5974ABD0EA78EE23CF7C940B315" pageId="0" pageNumber="45">
<emphasis id="C818DBDF5213997DBE5F1E3018B8346C" bold="true" pageId="0" pageNumber="45">Figure 6.</emphasis>
<taxonomicName id="745A8C6A7ECA6883621F691956A915E9" authorityName="Itagaki &amp; Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis didymospora" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="didymospora">
<emphasis id="8672B858E5B102BB2D0AB803B6FF1672" italics="true" pageId="0" pageNumber="45">Neobelonopsis didymospora</emphasis>
</taxonomicName>
(TNS-F-13501, holotype)
<emphasis id="8F9927DA38EB95EB031A3CA277669597" bold="true" pageId="0" pageNumber="45">A</emphasis>
fresh apothecia on decaying Bamboo culm
<emphasis id="3A399173EB69536C1914A561A2BEE1F1" bold="true" pageId="0" pageNumber="45">B</emphasis>
dried apothecia on decaying Bamboo culm
<emphasis id="D3A0364C34974793B6C486EE4E47AB2D" bold="true" pageId="0" pageNumber="45">C</emphasis>
fresh apothecia on decaying unidentified wood (TNS-F-86670)
<emphasis id="B5B370B4D9F8F84FDBBA106754DB2CCF" bold="true" pageId="0" pageNumber="45">D</emphasis>
dried apothecia on decaying unidentified wood (TNS-F-86670)
<emphasis id="7821F60A177D7E2DB332626E07E23694" bold="true" pageId="0" pageNumber="45">E</emphasis>
vertical section of the apothecium (in LA)
<emphasis id="B9A16386843E3BBC12471A3BCCACE6EE" bold="true" pageId="0" pageNumber="45">F</emphasis>
reflective vacuoles in fresh marginal cells (in water)
<emphasis id="4A116547EC72426C7722CAF826860E60" bold="true" pageId="0" pageNumber="45">G</emphasis>
vertical section at the basal apothecium (in lactic acid)
<emphasis id="36AC33885F2E9BDD8D8AB5F87445A838" bold="true" pageId="0" pageNumber="45">H</emphasis>
ascospores (in CB/LA)
<emphasis id="51FDF51D20E2D3E3998DD983FE560E91" bold="true" pageId="0" pageNumber="45">I</emphasis>
asci with ascospores (in CB/LA)
<emphasis id="B16291BFADABACABCD35DD58D08EB0B2" bold="true" pageId="0" pageNumber="45">J</emphasis>
paraphyses (in CB/LA)
<emphasis id="421AA1A878DCDBCA7A757B1241361C27" bold="true" pageId="0" pageNumber="45">K</emphasis>
fresh paraphyses with long refractive vacuoles (in water)
<emphasis id="08527390A1680C2246C9C017F5261A21" bold="true" pageId="0" pageNumber="45">L</emphasis>
blue-stained apical pore of ascus (in
<normalizedToken id="5192D07D6EB73B433D3AB3FFA971C42D" originalValue="Melzers">Melzer's</normalizedToken>
solution after 3% KOH pretreatment)
<emphasis id="D2A1951C650525E00233EDC8A6DE7C12" bold="true" pageId="0" pageNumber="45">M</emphasis>
crozier at the base of ascus (in CB/LA)
<emphasis id="2CE3E531B6300B0B878CCC02F4EAF37B" bold="true" pageId="0" pageNumber="45">N</emphasis>
three months old colony on PDA
<emphasis id="256AFAD5FB728C5A1853F93C0214FA6D" bold="true" pageId="0" pageNumber="45">O</emphasis>
clusters of conidiophores (in CB/LA)
<emphasis id="07F2968AA88B5754FA7E22C4C01D6331" bold="true" pageId="0" pageNumber="45">P-S</emphasis>
conidiophores with conidia (in CB/LA). Scale bars: 1 mm (
<emphasis id="89CEE74B146003E9BAE616F654099F13" bold="true" pageId="0" pageNumber="45">A-D</emphasis>
); 50
<normalizedToken id="B5D90540061C43D607B58D754D3FD861" originalValue="μm">μm</normalizedToken>
(
<emphasis id="0338A78FA8CF35045B57526021382D4C" bold="true" pageId="0" pageNumber="45">E</emphasis>
); 20
<normalizedToken id="7E800A56AB371E9C1610E2BA7CCFC620" originalValue="μm">μm</normalizedToken>
(
<emphasis id="39CA7A0A4BD4F4D3DF66002DAED6EC83" bold="true" pageId="0" pageNumber="45">O</emphasis>
), 10
<normalizedToken id="426A100A8366881F4FCAA205768C156B" originalValue="μm">μm</normalizedToken>
(
<emphasis id="F06E7908AB8E12BA1F876561C95A3A5F" bold="true" pageId="0" pageNumber="45">F-M, P-S</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection id="E9679E95E91F4E26861455A6F821E620" pageId="0" pageNumber="45" type="materials_examined">
<paragraph id="473586D4F86E86CA5E369FC09D5FFC49" pageId="0" pageNumber="45">Additional specimens examined.</paragraph>
<paragraph id="416A113DED45FFF2A8DF56DFDCE95AB9" pageId="0" pageNumber="45">
TNS-F-13509, Yakushima Island, Kagoshima Pref.,
<date id="4C12EBF885E888AD2A7BE76A7F405A61" value="2005-10-19">19 October 2005</date>
, on decaying wood of
<taxonomicName id="9E418CF5C946C9A5EECCDD58876B3072" class="Dicotyledoneae" family="Elaeocarpaceae" genus="Elaeocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Elaeocarpus japonicus" order="Malvales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="japonicus">
<emphasis id="F2CB9099585E01E6004F7D7BB49DCDD0" italics="true" pageId="0" pageNumber="45">Elaeocarpus japonicus</emphasis>
</taxonomicName>
, culture NBRC 115651; TNS-F-86178, Shishizuka Pond, Tsuchiura City, Ibaraki Pref.,
<date id="C314128D8F0A62FCB64765853D7EFEE2" value="2018-10-29">29 October 2018</date>
, on decaying wood of
<taxonomicName id="B9F61DE221CB79CDFDAE8B7DA3680506" class="Dicotyledoneae" family="Fabaceae" genus="Albizia" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Albizia julibrissin" order="Rosales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="julibrissin">
<emphasis id="FFC96AF5B4BBE2F0CB7CD69BA23DD8AF" italics="true" pageId="0" pageNumber="45">Albizia julibrissin</emphasis>
</taxonomicName>
, culture NBRC 115657; TNS-F-88720, Shirokanedai, Meguro Ward, Tokyo,
<date id="977F9618770FC0CC4DED854BEF612DD1" value="2018-07-06">6 July 2018</date>
, on dead stem of
<taxonomicName id="2DB94DB77AC03EDC4EDDAB850F195EB6" class="Monocotyledoneae" family="Arecaceae" genus="Trachycarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Trachycarpus fortunei" order="Principes" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="fortunei">
<emphasis id="42730A26181EDFAFE5A40707A526AE08" italics="true" pageId="0" pageNumber="45">Trachycarpus fortunei</emphasis>
</taxonomicName>
; TNS-F-86661 and TNS-F-86652, Yugashima, Izu City, Shizuoka Pref.,
<date id="B2DBF8F08EDFB15D8AFC1D6CB6F9229E" value="2021-10-15">15 October 2021</date>
on decaying culms of woody bamboos and unidentified wood, respectively; TNS-F-86670, Kawazu City, Kamo County, Shizuoka Pref.,
<date id="AC2E296AFFB82A412CCFD16D8F5EEA00" value="2021-10-16">16 October 2021</date>
, on unidentified decaying wood; TNS-F-86718, Mt. Katsuu, Nago City, Okinawa Pref.,
<date id="15AB76CFE4423D25B7808C8B50D19AC4" value="2021-10-27">27 October 2021</date>
, on decaying wood of
<taxonomicName id="16239E1EC471396108F187E16C3E8D59" class="Dicotyledoneae" family="Betulaceae" genus="Alnus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Alnus" order="Fagales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="genus">
<emphasis id="4BB09DB2BE9D23F4FBFDB19461DDB8A1" italics="true" pageId="0" pageNumber="45">Alnus</emphasis>
</taxonomicName>
sp.
</paragraph>
</subSubSection>
<subSubSection id="B4C55E0AC5AF0EED23D392302BECAF67" pageId="0" pageNumber="45" type="notes">
<paragraph id="6ED318C774C618C15811146A5681EFD6" pageId="0" pageNumber="45">Notes.</paragraph>
<paragraph id="580B877BDD8672B234E94818C19BABD7" pageId="0" pageNumber="45">
<taxonomicName id="147FC646DD06E75072A94B43F98C9FB4" authorityName="Itagaki &amp; Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis didymospora" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="didymospora">
<emphasis id="CB37A1C0732AB58519CCC7114F9E267E" italics="true" pageId="0" pageNumber="45">Neobelonopsis didymospora</emphasis>
</taxonomicName>
forms apothecia in autumn (October-December) and has a wide host range, but limited to woody plants, including woody bamboo.
<taxonomicName id="994652D9B6DCD9D776FBABA7C3C70684" authorityName="Itagaki &amp; Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis didymospora" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="didymospora">
<emphasis id="A29BFDBB0D7CD9305B99594347B32093" italics="true" pageId="0" pageNumber="45">Neobelonopsis didymospora</emphasis>
</taxonomicName>
forms its asexual stage only on CMA (Fig.
<figureCitation id="6C04AE46C411DAB9F627E8570C0955C6" captionStart="Figure 6" captionStartId="F6" captionText="Figure 6. Neobelonopsis didymospora (TNS-F- 13501, holotype) A fresh apothecia on decaying Bamboo culm B dried apothecia on decaying Bamboo culm C fresh apothecia on decaying unidentified wood (TNS-F- 86670) D dried apothecia on decaying unidentified wood (TNS-F- 86670) E vertical section of the apothecium (in LA) F reflective vacuoles in fresh marginal cells (in water) G vertical section at the basal apothecium (in lactic acid) H ascospores (in CB / LA) I asci with ascospores (in CB / LA) J paraphyses (in CB / LA) K fresh paraphyses with long refractive vacuoles (in water) L blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) M crozier at the base of ascus (in CB / LA) N three months old colony on PDA O clusters of conidiophores (in CB / LA) P-S conidiophores with conidia (in CB / LA). Scale bars: 1 mm (A-D); 50 μm (E); 20 μm (O), 10 μm (F-M, P-S)." figureDoi="10.3897/mycokeys.99.90117.figure6" httpUri="https://binary.pensoft.net/fig/892422" pageId="0" pageNumber="45">6O, S</figureCitation>
). This fungus is superficially similar to
<taxonomicName id="0D736B1E3FB2B2481074B8D29567E939" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. bicolor" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="bicolor">
<emphasis id="892C787619A632B7081FB744435907EE" italics="true" pageId="0" pageNumber="45">N. bicolor</emphasis>
</taxonomicName>
, but differs in fewer guttules in the cytoplasm.
</paragraph>
<paragraph id="0D399FD8288A4BFA877EEE9532C61DDA" pageId="0" pageNumber="45">
Based on a BLAST search of the GenBank database, the closest hits to the ITS sequences of
<taxonomicName id="01441C9F6303D91210BA551C72691BC4" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. didymospora" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="didymospora">
<emphasis id="6A202CC873BE7C4F4297EAB3274B09D1" italics="true" pageId="0" pageNumber="45">N. didymospora</emphasis>
</taxonomicName>
were three sequences of
<taxonomicName id="8AA154C6E6DFB378BB6CAF3779CDE1D7" authorityName="P.A.Karsten" authorityYear="1871" baseAuthorityName="E.M.Fries" class="Ascomycetes" family="Discomycetes" genus="Mollisia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Mollisia" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="genus">
<emphasis id="F3CE02548372D0E08A50F9493609604C" italics="true" pageId="0" pageNumber="45">Mollisia</emphasis>
</taxonomicName>
sp. from New Zealand collected from the dead frond of
<taxonomicName id="DC641E442FCE7BF7F0B35551398A5D65" authorityName="H.Wendl. &amp; Drude" authorityYear="1878" baseAuthorityName="Soland ex G.Forst." class="Liliopsida" family="Arecaceae" genus="Rhopalostylis" higherTaxonomySource="IPNI" kingdom="Plantae" lsidName="Rhopalostylis sapida" order="Arecales" pageId="0" pageNumber="45" phylum="Magnoliophyta" rank="species" species="sapida">
<emphasis id="4DC00F8FE7954BDC14B6515355CA8C7C" italics="true" pageId="0" pageNumber="45">Rhopalostylis sapida</emphasis>
</taxonomicName>
[MG195516; Identities=553/554 (99.8%), no gaps], fallen unidentified wood [MG195517; Identities=551/554 (99.5%), one gap], and fallen wood of
<taxonomicName id="FC33BDE9398131558105DE62F75BFC5D" authorityName="Lindsay" authorityYear="1868" class="Dicotyledoneae" family="Coriariaceae" genus="Coriaria" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Coriaria arborea" order="Sapindales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="arborea">
<emphasis id="2412D1D0F6400B825CD69A6FE9305871" italics="true" pageId="0" pageNumber="45">Coriaria arborea</emphasis>
</taxonomicName>
[MG195518; Identities=511/511 (100%), no gaps]. The presence of these sequence data suggests that distribution of
<taxonomicName id="B68EA789D4C4EFC2A46DD9B812B9DFDC" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. didymospora" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="didymospora">
<emphasis id="89DFE282B854F48E25A2DCADD694D19B" italics="true" pageId="0" pageNumber="45">N. didymospora</emphasis>
</taxonomicName>
is not limited in Japan, but also in New Zealand.
</paragraph>
</subSubSection>
</treatment>
</document>