98 lines
9.8 KiB
XML
98 lines
9.8 KiB
XML
<document id="415909351CDFA9EEFA377C84C2161532" ID-DOI="10.1016/j.phytochem.2021.112667" ID-ISSN="1873-3700" ID-Zenodo-Dep="8292146" IM.bibliography_approvedBy="valdenar" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="valdenar" IM.treatments_approvedBy="felipe" checkinTime="1693247767738" checkinUser="felipe" docAuthor="Chen, Jinjun, Liu, Zihao, Liu, Yisong, Zhang, Xuewen & Zeng, Jianguo" docDate="2021" docId="039087D9877C031C953900EAA07CFAC2" docLanguage="en" docName="Phytochemistry.184.112667.pdf" docOrigin="Phytochemistry (112667) 184" docSource="http://dx.doi.org/10.1016/j.phytochem.2021.112667" docStyle="DocumentStyle:F36D69FC8B198FBE91029DF9C24697D3.5:Phytochemistry.2020-.journal_article" docStyleId="F36D69FC8B198FBE91029DF9C24697D3" docStyleName="Phytochemistry.2020-.journal_article" docStyleVersion="5" docTitle="Macleaya cordata R. Br." docType="treatment" docVersion="3" lastPageNumber="2" masterDocId="FFA9FFA1877D031D955D0219A137FFAE" masterDocTitle="Preliminary investigations on the pathogenesis-related protein expression profile of the medicinal herb Macleaya cordata and anti-bacterial properties of recombinant proteins" masterLastPageNumber="9" masterPageNumber="1" pageNumber="2" updateTime="1693414541625" updateUser="valdenar">
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<mods:title id="5EFE35FC9D4FDB0F3FF6AEC416F5E7E9">Preliminary investigations on the pathogenesis-related protein expression profile of the medicinal herb Macleaya cordata and anti-bacterial properties of recombinant proteins</mods:title>
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<mods:namePart id="120E3B6F5A6E9E01A39A3D934E1CD601">Chen, Jinjun</mods:namePart>
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<mods:affiliation id="318D89D0FDFB81BFC578967958CED41F">* & College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, 410128, China</mods:affiliation>
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<mods:namePart id="591F5CCCBDD18D612CB3D32AE8264C56">Liu, Zihao</mods:namePart>
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<mods:namePart id="152D1EBBB89FE2018227FE6C0EDF7E28">Liu, Yisong</mods:namePart>
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<mods:namePart id="050473C7217028094059D5B34860C73F">Zhang, Xuewen</mods:namePart>
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<mods:namePart id="4F08F28500690D2AD03B8617A60C2385">Zeng, Jianguo</mods:namePart>
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<mods:title id="D957CCEAC13C56E869EDAAEB92D19E51">Phytochemistry</mods:title>
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<mods:date id="71FBB47BE9279DECD00E57F22D232B91">2021</mods:date>
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<mods:number id="C7E21EB21C32BA33C8CDC6D527C1CD45">2021-04-30</mods:number>
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<mods:identifier id="CD958B7C9A0A6DEF0783950051BBFE8E" type="ISSN">1873-3700</mods:identifier>
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<treatment id="039087D9877C031C953900EAA07CFAC2" LSID="urn:lsid:plazi:treatment:039087D9877C031C953900EAA07CFAC2" httpUri="http://treatment.plazi.org/id/039087D9877C031C953900EAA07CFAC2" lastPageNumber="2" pageId="1" pageNumber="2">
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<subSubSection id="C3236544877C031C953900EAA3D8FCA8" box="[100,751,755,775]" pageId="1" pageNumber="2" type="nomenclature">
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<paragraph id="8B8636CF877C031C953900EAA3D8FCA8" blockId="1.[100,751,755,775]" box="[100,751,755,775]" pageId="1" pageNumber="2">
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<heading id="D0CE81A3877C031C953900EAA3D8FCA8" bold="true" box="[100,751,755,775]" centered="true" fontSize="36" level="1" pageId="1" pageNumber="2" reason="1">
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<emphasis id="B94DEADD877C031C953900EAA3D8FCA8" bold="true" box="[100,751,755,775]" italics="true" pageId="1" pageNumber="2">
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2.1. An overview of the analysis of
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<taxonomicName id="4C394D4C877C031C94F600EDA338FCA8" ID-CoL="3WZNV" ID-ENA="56857" authority="(Willd.) R. Br." authorityName="R. Br." baseAuthorityName="Willd." box="[427,527,755,775]" class="Magnoliopsida" family="Papaveraceae" genus="Macleaya" kingdom="Plantae" order="Ranunculales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="species" species="cordata">M. cordata</taxonomicName>
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(willd.) R. Br. leaf ESTs
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</emphasis>
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</heading>
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</paragraph>
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<subSubSection id="C3236544877C031C95D90132A07CFAC2" pageId="1" pageNumber="2" type="description">
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<paragraph id="8B8636CF877C031C95D90132A049FBFB" blockId="1.[100,770,811,1388]" pageId="1" pageNumber="2">
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The cDNA library from
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<taxonomicName id="4C394D4C877C031C943C0132A0F3FC90" box="[353,452,811,830]" class="Magnoliopsida" family="Papaveraceae" genus="Macleaya" kingdom="Plantae" order="Ranunculales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="species" species="cordata">
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<emphasis id="B94DEADD877C031C943C0132A0F3FC90" bold="true" box="[353,452,811,830]" italics="true" pageId="1" pageNumber="2">M. cordata</emphasis>
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leaf was non-normalized, and the titer was 1.0 ×
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<superScript id="7C4C9B87877C031C945D015EA016FCE1" attach="left" box="[256,289,833,858]" fontSize="6" pageId="1" pageNumber="2">106</superScript>
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cfu/mL with recombinant clones. After the poorquality sequences were discarded, 511 high-quality ESTs were used to investigate the gene expression profile of
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<taxonomicName id="4C394D4C877C031C94A30166A354FC3F" box="[510,611,894,914]" class="Magnoliopsida" family="Papaveraceae" genus="Macleaya" kingdom="Plantae" order="Ranunculales" pageId="1" pageNumber="2" phylum="Tracheophyta" rank="species" species="cordata">
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<emphasis id="B94DEADD877C031C94A30166A354FC3F" bold="true" box="[510,611,894,914]" italics="true" pageId="1" pageNumber="2">M. cordata</emphasis>
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</taxonomicName>
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leaf. The initial sequences were grouped into 364 non-redundancy sequences, of which 78 clusters exhibited more than one EST and 286 singlets (Supplementary Table 1). The average length of the non-redundant sequence was 513 nucleotides (ranging from 105 to 1013 bp) (Supplementary
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<figureCitation id="13022A4A877C031C95390613A180FBB3" box="[100,183,1034,1053]" captionStart="Fig" captionStartId="2.[100,130,1913,1930]" captionTargetBox="[310,1275,1696,1878]" captionTargetId="figure-1257@2.[301,1287,1686,1886]" captionTargetPageId="2" captionText="Fig. 1. The alignment analysis of McDef1-5. The underlined amino acids represent the signal sequence predicted with SignalP. The cysteines are marked in yellow background and the putative disulfide bonds are also shown. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)" figureDoi="http://doi.org/10.5281/zenodo.8292148" httpUri="https://zenodo.org/record/8292148/files/figure.png" pageId="1" pageNumber="2">Figure 1</figureCitation>
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). Additionally, the newly discovered EST sequences of the cDNA library were submitted to Genbank with the accession number from JK752513 to JK752907.
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</paragraph>
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<paragraph id="8B8636CF877C031C95D90647A07CFAC2" blockId="1.[100,770,811,1388]" pageId="1" pageNumber="2">With the development of high-throughput next-generation sequencing technologies, sequencing the transcriptomes of medicinal plants with immense pace has become a hot pursuit, generating more generalized insights. However, de novo assembly is a big challenge, especially for the assembly of cysteine-rich defensins, because these peptides are hypervariable sequences using conserved scaffolds. The cDNA library with ESTs Sanger sequencing approach has been verified to be a rapid and reliable method for uncovering undescribed genes, and the interesting candidates provide the gene templates for subsequent recombinant expression.</paragraph>
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</subSubSection>
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</treatment>
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</document> |