146 lines
16 KiB
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146 lines
16 KiB
XML
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<mods:title id="62C203989971B34AB9479D92F693A116">De novo root transcriptome of a medicinally important rare tree Oroxylum indicum for characterization of the flavonoid biosynthesis pathway</mods:title>
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<mods:name id="B97E2ED618F2D088763601F7D17A2362" type="personal">
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<mods:namePart id="EAE208D1A030E092558F9155FCD0F884">Deshmukh, Aaditi B.</mods:namePart>
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<mods:affiliation id="C89D78FE8297A9B4D74DFCA6F20C8105">∗ & Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University, Ganeshkhind Road, Pune, 411007, Maharashtra, India</mods:affiliation>
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<mods:namePart id="4A6BD6492C479297D63D3B6364218ED9">Datir, Sagar S.</mods:namePart>
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<mods:namePart id="1C18E1A0ADA29A4C1B779E0798BE1E97">Bhonde, Yogesh</mods:namePart>
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<mods:namePart id="593BB0A8D8ADE67C9E393E98FE233E84">Kelkar, Natasha</mods:namePart>
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<mods:namePart id="76C18560E7CAE2CB44215B12D367AE02">Samdani, Pawan</mods:namePart>
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<mods:namePart id="1AD2F8C430B011510DAE4821D1120327">Tamhane, Vaijayanti A.</mods:namePart>
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<mods:title id="31C11E70DC69FC054FD0ECE826B60149">Phytochemistry</mods:title>
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<mods:part id="54F9855161599719A69DAEF0823740E8">
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<mods:date id="BBCCBC4B56A71C2355A1BDA51714354F">2018</mods:date>
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<mods:number id="BFF021432448C7357A6F47C44D87A092">2018-12-31</mods:number>
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<mods:number id="E30FAA84F2F775F2B5417893AE556240">156</mods:number>
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<mods:url id="C8F62AC838B310138CE292D39A7F8A21">http://dx.doi.org/10.1016/j.phytochem.2018.09.013</mods:url>
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<mods:classification id="E26FD8E9D01B698B51DFE8CA2658AFDB">journal article</mods:classification>
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<mods:identifier id="AA55C8B04A862E3B597A243FC853F97E" type="DOI">10.1016/j.phytochem.2018.09.013</mods:identifier>
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<mods:identifier id="2F1E2A3323E1840638EBDE5403A82763" type="ISSN">1873-3700</mods:identifier>
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<mods:identifier id="C452A901D20DED252697020A3148C8D1" type="Zenodo-Dep">10484692</mods:identifier>
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<treatment id="03881B7683597F01FF9BE8B681C6F8D9" LSID="urn:lsid:plazi:treatment:03881B7683597F01FF9BE8B681C6F8D9" httpUri="http://treatment.plazi.org/id/03881B7683597F01FF9BE8B681C6F8D9" lastPageNumber="206" pageId="5" pageNumber="206">
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<subSubSection id="C33BF9EB83597F01FF9BE8B68284FB58" pageId="5" pageNumber="206" type="nomenclature">
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<paragraph id="8B9EAA6083597F01FF9BE8B68284FB58" blockId="5.[100,695,1223,1242]" lastBlockId="5.[100,751,1251,1270]" pageId="5" pageNumber="206">
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<emphasis id="B955767283597F01FF9BE8B68284FB58" bold="true" italics="true" pageId="5" pageNumber="206">
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<heading id="D0D61D0C83597F01FF9BE8B682DCFB74" bold="true" box="[100,695,1223,1242]" fontSize="36" level="1" pageId="5" pageNumber="206" reason="1">2.7. Expression pattern of selected phenylpropanoid and flavonoid</heading>
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<heading id="D0D61D0C83597F01FF9BE8928284FB58" box="[100,751,1251,1270]" centered="true" fontSize="8" level="3" pageId="5" pageNumber="206" reason="8">
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biosynthesis pathway genes in the young and mature roots of
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<taxonomicName id="4C21D1E383597F01FD73E8928284FB58" ID-CoL="99R85" authority="(L.) Kurz" box="[652,751,1251,1270]" class="Magnoliopsida" family="Bignoniaceae" genus="Oroxylum" kingdom="Plantae" order="Lamiales" pageId="5" pageNumber="206" phylum="Tracheophyta" rank="species" species="indicum">O. indicum</taxonomicName>
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</heading>
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</emphasis>
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</paragraph>
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<subSubSection id="C33BF9EB83597F01FF7AE96B81C6F8D9" pageId="5" pageNumber="206" type="description">
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<paragraph id="8B9EAA6083597F01FF7AE96B81C6F8D9" blockId="5.[100,770,1306,1911]" pageId="5" pageNumber="206">
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Relative expression levels of seven genes namely, cinnamyl alcohol dehydrogenase (
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<emphasis id="B955767283597F01FEF8E947813AFAE7" bold="true" box="[263,337,1334,1353]" italics="true" pageId="5" pageNumber="206">OinCAD</emphasis>
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), 4-coumarate–CoA ligase (
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<emphasis id="B955767283597F01FD93E94782DAFAE7" bold="true" box="[620,689,1334,1353]" italics="true" pageId="5" pageNumber="206">Oin4CL</emphasis>
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), transcinnamate 4-monooxygenase (
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<emphasis id="B955767283597F01FE74E92381BFFACB" bold="true" box="[395,468,1362,1381]" italics="true" pageId="5" pageNumber="206">OinC4H</emphasis>
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), chalcone synthase (
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<emphasis id="B955767283597F01FD53E923829FFACB" bold="true" box="[684,756,1362,1381]" italics="true" pageId="5" pageNumber="206">OinCHS</emphasis>
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), flavone synthase (
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<emphasis id="B955767283597F01FEF1E91F8138FA2F" bold="true" box="[270,339,1390,1409]" italics="true" pageId="5" pageNumber="206">OinFNS</emphasis>
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), chalcone isomerase (
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<emphasis id="B955767283597F01FDD4E91F8205FA2F" bold="true" box="[555,622,1390,1409]" italics="true" pageId="5" pageNumber="206">OinCHI</emphasis>
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) and flavanone 3-dioxygenase (
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<emphasis id="B955767283597F01FF04E9FB8129FA33" bold="true" box="[251,322,1418,1437]" italics="true" pageId="5" pageNumber="206">OinF3H</emphasis>
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) were determined by a quantitative real time polymerase chain reaction (qRT-PCR) (
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<figureCitation id="131AB6E583597F01FE2CE9D78263FA17" box="[467,520,1446,1465]" captionStart="Fig" captionStartId="6.[100,130,845,862]" captionTargetBox="[268,1315,156,772]" captionTargetId="graphics-539@6.[327,1313,162,685]" captionTargetPageId="6" captionText="Fig. 4. Expression pattern of selected genes from phenylpropanoid and flavonoid biosynthetic pathway using qRT-PCR. Seven genes involved in phenylpropanoid and flavonoid biosynthesis namely, cinnamyl alcohol dehydrogenase (OinCAD), 4-coumarate–CoA ligase (Oin4CL), Trans-cinnamate 4-monooxygenase (OinC4H), chalcone synthase (OinCHS), flavone synthase (OinFNS), chalcone isomerase (OinCHI) and flavanone 3-dioxygenase (OinF3H) were selected for qRT-PCR analysis. The results represent the means ± standard error of experiments performed in triplicate. The data (n = 5) was analyzed using Student's t-test (*p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001)." figureDoi="http://doi.org/10.5281/zenodo.10484700" httpUri="https://zenodo.org/record/10484700/files/figure.png" pageId="5" pageNumber="206">Fig. 4</figureCitation>
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) in young and old roots of
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<taxonomicName id="4C21D1E383597F01FF9BE9B380A2FA7B" box="[100,201,1474,1493]" class="Magnoliopsida" family="Bignoniaceae" genus="Oroxylum" kingdom="Plantae" order="Lamiales" pageId="5" pageNumber="206" phylum="Tracheophyta" rank="species" species="indicum">
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<emphasis id="B955767283597F01FF9BE9B380A2FA7B" bold="true" box="[100,201,1474,1493]" italics="true" pageId="5" pageNumber="206">O. indicum</emphasis>
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</taxonomicName>
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. The expression level of
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<emphasis id="B955767283597F01FE40E9B38262FA7B" bold="true" box="[447,521,1474,1493]" italics="true" pageId="5" pageNumber="206">OinCAD</emphasis>
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was not significantly different in the two tissues.
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<collectionCode id="ED3032A583597F01FEA5E9AF8103FA5F" box="[346,360,1502,1521]" country="USA" lsid="urn:lsid:biocol.org:col:15406" name="Harvard University - Arnold Arboretum" pageId="5" pageNumber="206" type="Herbarium">A</collectionCode>
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two fold higher expression of
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<emphasis id="B955767283597F01FD6DE9AF82BDFA5F" bold="true" box="[658,726,1502,1521]" italics="true" pageId="5" pageNumber="206">Oin4CL</emphasis>
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was detected in old roots as compared to young roots. The expression of
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<emphasis id="B955767283597F01FF9BEA6780C6F987" bold="true" box="[100,173,1558,1577]" italics="true" pageId="5" pageNumber="206">OinC4H</emphasis>
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was lower in both the young and old root of
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<taxonomicName id="4C21D1E383597F01FDB1EA6782C4F987" box="[590,687,1558,1577]" class="Magnoliopsida" family="Bignoniaceae" genus="Oroxylum" kingdom="Plantae" order="Lamiales" pageId="5" pageNumber="206" phylum="Tracheophyta" rank="species" species="indicum">
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<emphasis id="B955767283597F01FDB1EA6782C4F987" bold="true" box="[590,687,1558,1577]" italics="true" pageId="5" pageNumber="206">O. indicum</emphasis>
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</taxonomicName>
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.
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<emphasis id="B955767283597F01FD45EA678369F987" bold="true" box="[698,770,1558,1577]" italics="true" pageId="5" pageNumber="206">OinCHS</emphasis>
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showed around a four fold higher expression in the old root as compared to the young root (
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<figureCitation id="131AB6E583597F01FEB0EA3C81E8F9CE" box="[335,387,1613,1632]" captionStart="Fig" captionStartId="6.[100,130,845,862]" captionTargetBox="[268,1315,156,772]" captionTargetId="graphics-539@6.[327,1313,162,685]" captionTargetPageId="6" captionText="Fig. 4. Expression pattern of selected genes from phenylpropanoid and flavonoid biosynthetic pathway using qRT-PCR. Seven genes involved in phenylpropanoid and flavonoid biosynthesis namely, cinnamyl alcohol dehydrogenase (OinCAD), 4-coumarate–CoA ligase (Oin4CL), Trans-cinnamate 4-monooxygenase (OinC4H), chalcone synthase (OinCHS), flavone synthase (OinFNS), chalcone isomerase (OinCHI) and flavanone 3-dioxygenase (OinF3H) were selected for qRT-PCR analysis. The results represent the means ± standard error of experiments performed in triplicate. The data (n = 5) was analyzed using Student's t-test (*p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001)." figureDoi="http://doi.org/10.5281/zenodo.10484700" httpUri="https://zenodo.org/record/10484700/files/figure.png" pageId="5" pageNumber="206">Fig. 4</figureCitation>
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).
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<emphasis id="B955767283597F01FE68EA3C81D4F9CE" bold="true" box="[407,447,1613,1632]" italics="true" pageId="5" pageNumber="206">CHS</emphasis>
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is the first rate-limiting enzyme in flavonoid biosynthesis pathway and higher levels of the enzyme is directly correlated with the higher level of flavonoids (Zuk et al., 2016). On the contrary, expression of
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<emphasis id="B955767283597F01FE7EEAD081AFF91A" bold="true" box="[385,452,1697,1716]" italics="true" pageId="5" pageNumber="206">OinCHI</emphasis>
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,
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<emphasis id="B955767283597F01FE2FEAD0827EF91A" bold="true" box="[464,533,1697,1716]" italics="true" pageId="5" pageNumber="206">OinFNS</emphasis>
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and
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<emphasis id="B955767283597F01FDBDEAD082E3F91A" bold="true" box="[578,648,1697,1716]" italics="true" pageId="5" pageNumber="206">OinF3H</emphasis>
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were at least ten fold higher in young roots as compared to old roots of
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<taxonomicName id="4C21D1E383597F01FD61EACC8369F97E" box="[670,770,1725,1744]" class="Magnoliopsida" family="Bignoniaceae" genus="Oroxylum" kingdom="Plantae" order="Lamiales" pageId="5" pageNumber="206" phylum="Tracheophyta" rank="species" species="indicum">
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<emphasis id="B955767283597F01FD61EACC8369F97E" bold="true" box="[670,770,1725,1744]" italics="true" pageId="5" pageNumber="206">O. indicum</emphasis>
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</taxonomicName>
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(
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<figureCitation id="131AB6E583597F01FF93EAA880C9F942" box="[108,162,1753,1772]" captionStart="Fig" captionStartId="6.[100,130,845,862]" captionTargetBox="[268,1315,156,772]" captionTargetId="graphics-539@6.[327,1313,162,685]" captionTargetPageId="6" captionText="Fig. 4. Expression pattern of selected genes from phenylpropanoid and flavonoid biosynthetic pathway using qRT-PCR. Seven genes involved in phenylpropanoid and flavonoid biosynthesis namely, cinnamyl alcohol dehydrogenase (OinCAD), 4-coumarate–CoA ligase (Oin4CL), Trans-cinnamate 4-monooxygenase (OinC4H), chalcone synthase (OinCHS), flavone synthase (OinFNS), chalcone isomerase (OinCHI) and flavanone 3-dioxygenase (OinF3H) were selected for qRT-PCR analysis. The results represent the means ± standard error of experiments performed in triplicate. The data (n = 5) was analyzed using Student's t-test (*p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001)." figureDoi="http://doi.org/10.5281/zenodo.10484700" httpUri="https://zenodo.org/record/10484700/files/figure.png" pageId="5" pageNumber="206">Fig. 4</figureCitation>
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). The higher expression of
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<emphasis id="B955767283597F01FE58EAA881A1F942" bold="true" box="[423,458,1753,1772]" italics="true" pageId="5" pageNumber="206">CHI</emphasis>
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in young roots may be linked to its role in auxin movement leading to plant growth and development (
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<bibRefCitation id="EFB0D79183597F01FF93EB608138F88A" author="Peer, W. A. & Murphy, A. S." box="[108,339,1809,1828]" pageId="5" pageNumber="206" pagination="556 - 563" refId="ref13113" refString="Peer, W. A., Murphy, A. S., 2007. Flavonoids and auxin transport: modulators or regulators? Trends Plant Sci. 12, 556 - 563." type="journal article" year="2007">Peer and Murphy, 2007</bibRefCitation>
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). Moreover, there are possibly different isoforms of the flavonoid biosynthesis genes which display spatial and temporal regulation, as reflected by their differential expression in young and old roots of
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<taxonomicName id="4C21D1E383597F01FEBBEB1581CCF8D9" box="[324,423,1892,1911]" class="Magnoliopsida" family="Bignoniaceae" genus="Oroxylum" kingdom="Plantae" order="Lamiales" pageId="5" pageNumber="206" phylum="Tracheophyta" rank="species" species="indicum">
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<emphasis id="B955767283597F01FEBBEB1581CCF8D9" bold="true" box="[324,423,1892,1911]" italics="true" pageId="5" pageNumber="206">O. indicum</emphasis>
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</taxonomicName>
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.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |