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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.95.98969" ID-Pensoft-Pub="1314-4049-95-209" ID-Pensoft-UUID="C5B9D3498B9758A7A53810DC362F99A2" ModsDocID="1314-4049-95-209" checkinTime="1677749816561" checkinUser="pensoft" docAuthor="Zhu, Ya-Quan, Ma, Chun-Yan, Xue, Han, Piao, Chun-Gen, Li, Yong &amp; Jiang, Ning" docDate="2023" docId="1BC54B142E2C59528A5A3DC760DCB39F" docLanguage="en" docName="MycoKeys 95: 209-228" docOrigin="MycoKeys 95" docPubDate="2023-03-01" docSource="http://dx.doi.org/10.3897/mycokeys.95.98969" docTitle="Diaporthe smilacicola Y. Q. Zhu &amp; Ning Jiang 2023, sp. nov." docType="treatment" docVersion="1" id="C5B9D3498B9758A7A53810DC362F99A2" lastPageNumber="209" masterDocId="C5B9D3498B9758A7A53810DC362F99A2" masterDocTitle="Two new species of Diaporthe (Diaporthaceae, Diaporthales) in China" masterLastPageNumber="228" masterPageNumber="209" pageNumber="209" updateTime="1677749816561" updateUser="pensoft">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Two new species of Diaporthe (Diaporthaceae, Diaporthales) in China</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Zhu, Ya-Quan</mods:namePart>
<mods:affiliation>Key Laboratory of Biodiversity Conservation of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Ma, Chun-Yan</mods:namePart>
<mods:affiliation>Natural Resources and Planning Bureau of Rizhao City, Rizhao 276827, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Xue, Han</mods:namePart>
<mods:affiliation>Key Laboratory of Biodiversity Conservation of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Piao, Chun-Gen</mods:namePart>
<mods:affiliation>Key Laboratory of Biodiversity Conservation of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Li, Yong</mods:namePart>
<mods:affiliation>Key Laboratory of Biodiversity Conservation of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jiang, Ning</mods:namePart>
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-9656-8500</mods:nameIdentifier>
<mods:affiliation>Key Laboratory of Biodiversity Conservation of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China</mods:affiliation>
<mods:nameIdentifier type="email">n.jiang@caf.ac.cn</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2023</mods:date>
<mods:detail type="pubDate">
<mods:number>2023-03-01</mods:number>
</mods:detail>
<mods:detail type="volume">
<mods:number>95</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>209</mods:start>
<mods:end>228</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.95.98969</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.95.98969</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-95-209</mods:identifier>
<mods:identifier type="Pensoft-UUID">C5B9D3498B9758A7A53810DC362F99A2</mods:identifier>
</mods:mods>
<subSection pageId="0" pageNumber="209" type="results">
<treatment LSID="urn:lsid:plazi:treatment:1BC54B142E2C59528A5A3DC760DCB39F" httpUri="http://treatment.plazi.org/id/1BC54B142E2C59528A5A3DC760DCB39F" lastPageNumber="209" pageId="0" pageNumber="209">
<subSubSection pageId="0" pageNumber="209" type="nomenclature">
<paragraph pageId="0" pageNumber="209">
<taxonomicName LSID="1BC54B14-2E2C-5952-8A5A-3DC760DCB39F" authority="Y. Q. Zhu &amp; Ning Jiang" authorityName="Y. Q. Zhu &amp; Ning Jiang" authorityYear="2023" class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe smilacicola" order="Diaporthales" pageId="0" pageNumber="209" phylum="Ascomycota" rank="species" species="smilacicola" status="sp. nov.">Diaporthe smilacicola Y.Q. Zhu &amp; Ning Jiang</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="209">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="209" type="description">
<paragraph pageId="0" pageNumber="209">
<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Morphology of Diaporthe smilacicola A colonies on PDA, MEA and SNA at 25 ° C after 2 weeks B leaf spots on the host surface C conidiomata formed on the PDA D, E conidiogenous cells with attached alpha conidia F-G alpha conidia. Scale bars: 200 µm (C); 10 µm (D-G)." figureDoi="10.3897/mycokeys.95.98969.figure4" httpUri="https://binary.pensoft.net/fig/817930" pageId="0" pageNumber="209">Fig. 4</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="209" type="etymology">
<paragraph pageId="0" pageNumber="209">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="209">
Named after the host genus,
<taxonomicName class="Liliopsida" family="Smilacaceae" genus="Smilax" kingdom="Plantae" lsidName="Smilax" order="Liliales" pageId="0" pageNumber="209" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="209">Smilax</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="209" type="description">
<paragraph pageId="0" pageNumber="209">Description.</paragraph>
<paragraph pageId="0" pageNumber="209">
<emphasis bold="true" italics="true" pageId="0" pageNumber="209">Leaf spots</emphasis>
subcircular to irregular, pale brown to brown, with dark brown margin.
<emphasis bold="true" italics="true" pageId="0" pageNumber="209">Conidiomata</emphasis>
pycnidial, scattered, subglobose to globose, black, erumpent, exuding faint yellow translucent conidial droplets from central ostioles, 150-350
<normalizedToken originalValue="μm">μm</normalizedToken>
diam.
<emphasis bold="true" italics="true" pageId="0" pageNumber="209">Conidiogenous cells</emphasis>
11-16.2
<normalizedToken originalValue="×">x</normalizedToken>
1.8-2.4
<normalizedToken originalValue="μm">μm</normalizedToken>
, hyaline, phialidic, cylindrical, terminal, slightly tapering towards the apex.
<emphasis bold="true" italics="true" pageId="0" pageNumber="209">Alpha conidia</emphasis>
5.7-9.7
<normalizedToken originalValue="×">x</normalizedToken>
2.0-3.5
<normalizedToken originalValue="μm">μm</normalizedToken>
(mean = 7.8
<normalizedToken originalValue="×">x</normalizedToken>
2.6
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 50), hyaline, aseptate, smooth, guttulate, ellipsoidal to oblong ellipsoidal, with both ends obtuse.
<emphasis bold="true" italics="true" pageId="0" pageNumber="209">Beta conidia and gamma conidia</emphasis>
not observed.
<emphasis bold="true" italics="true" pageId="0" pageNumber="209">Sexual morph</emphasis>
not observed.
</paragraph>
<caption doi="10.3897/mycokeys.95.98969.figure4" httpUri="https://binary.pensoft.net/fig/817930" pageId="0" pageNumber="209" start="Figure 4" startId="F4">
<paragraph pageId="0" pageNumber="209">
<emphasis bold="true" pageId="0" pageNumber="209">Figure 4.</emphasis>
Morphology of
<taxonomicName authorityName="Y. Q. Zhu &amp; Ning Jiang" authorityYear="2023" class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe smilacicola" order="Diaporthales" pageId="0" pageNumber="209" phylum="Ascomycota" rank="species" species="smilacicola">
<emphasis italics="true" pageId="0" pageNumber="209">Diaporthe smilacicola</emphasis>
</taxonomicName>
<emphasis bold="true" pageId="0" pageNumber="209">A</emphasis>
colonies on PDA, MEA and SNA at 25 °C after 2 weeks
<emphasis bold="true" pageId="0" pageNumber="209">B</emphasis>
leaf spots on the host surface
<emphasis bold="true" pageId="0" pageNumber="209">C</emphasis>
conidiomata formed on the PDA
<emphasis bold="true" pageId="0" pageNumber="209">D, E</emphasis>
conidiogenous cells with attached alpha conidia
<emphasis bold="true" pageId="0" pageNumber="209">F-G</emphasis>
alpha conidia. Scale bars: 200
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="209">C</emphasis>
); 10
<normalizedToken originalValue="µm">µm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="209">D-G</emphasis>
).
</paragraph>
</caption>
</subSubSection>
<subSubSection pageId="0" pageNumber="209" type="description">
<paragraph pageId="0" pageNumber="209">Culture characters.</paragraph>
<paragraph pageId="0" pageNumber="209">Colonies on PDA flat, with flocculent aerial mycelium and crenate edge, white to gray, reaching a 90 mm diameter after 14 days at 25 °C, forming black conidiomata with black conidial masses; on MEA flat, spreading, with flocculent aerial mycelium forming concentric rings, off-white to luteous, reaching a 90 mm diameter after 14 days at 25 °C; on SNA flat, spreading, with flocculent aerial mycelium forming concentric rings and entire edge, white, reaching a 90 mm diameter after 14 days at 25 °C.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="209" type="materials_examined">
<paragraph pageId="0" pageNumber="209">Materials examined.</paragraph>
<paragraph pageId="0" pageNumber="209">
<materialsCitation collectingDate="2020-11-02" country="China" county="Changsha County" location="Kaihui Town" municipality="Changsha City" specimenCount="1" stateProvince="Hunan Province" typeStatus="holotype">
<collectingCountry name="China">China</collectingCountry>
,
<collectingRegion country="China" name="Hunan">Hunan Province</collectingRegion>
,
<collectingMunicipality>Changsha City</collectingMunicipality>
,
<collectingCounty>Changsha County</collectingCounty>
,
<location LSID="urn:lsid:plazi:treatment:1BC54B142E2C59528A5A3DC760DCB39F:80FDA34D575111FCE30F7C2AD44E33FC" country="China" county="Changsha County" municipality="Changsha City" name="Kaihui Town" stateProvince="Hunan Province">Kaihui Town</location>
, on leaf spots of
<taxonomicName class="Liliopsida" family="Smilacaceae" genus="Smilax" kingdom="Plantae" lsidName="Smilax glabra" order="Liliales" pageId="0" pageNumber="209" phylum="Tracheophyta" rank="species" species="glabra">
<emphasis italics="true" pageId="0" pageNumber="209">Smilax glabra</emphasis>
</taxonomicName>
,
<collectingDate value="2020-11-02">2 November 2020</collectingDate>
, Ning Jiang (
<typeStatus>holotype</typeStatus>
CAF 800070; ex-holotype culture CFCC 54582)
</materialsCitation>
.
<materialsCitation collectingDate="2020-11-02" country="China" location="Shaoshan City" specimenCount="1" stateProvince="Hunan Province" typeStatus="holotype">
<collectingRegion country="China" name="Hunan">Hunan Province</collectingRegion>
,
<location LSID="urn:lsid:plazi:treatment:1BC54B142E2C59528A5A3DC760DCB39F:0C82551F3AA461C18327B3DCFCC9F369" country="China" name="Shaoshan City" stateProvince="Hunan Province">Shaoshan City</location>
, on leaf spots of
<taxonomicName class="Liliopsida" family="Smilacaceae" genus="Smilax" kingdom="Plantae" lsidName="Smilax glabra" order="Liliales" pageId="0" pageNumber="209" phylum="Tracheophyta" rank="species" species="glabra">
<emphasis italics="true" pageId="0" pageNumber="209">Smilax glabra</emphasis>
</taxonomicName>
,
<collectingDate value="2020-11-02">2 November 2020</collectingDate>
, Ning Jiang (cultures CFCC 58764 and CFCC 58765)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="209" type="notes">
<paragraph pageId="0" pageNumber="209">Notes.</paragraph>
<paragraph pageId="0" pageNumber="209">
Three
<taxonomicName authorityName="Nitschke &amp; Pyrenomyc.Germ" authorityYear="1870" class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe" order="Diaporthales" pageId="0" pageNumber="209" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="209">Diaporthe</emphasis>
</taxonomicName>
isolates representing
<taxonomicName lsidName="D. smilacicola" pageId="0" pageNumber="209" rank="species" species="smilacicola">
<emphasis italics="true" pageId="0" pageNumber="209">D. smilacicola</emphasis>
</taxonomicName>
formed a well-supported clade (ML/BI = 100/1), and appear to be distinct from the other
<taxonomicName authorityName="Nitschke &amp; Pyrenomyc.Germ" authorityYear="1870" class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe" order="Diaporthales" pageId="0" pageNumber="209" phylum="Ascomycota" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="209">Diaporthe</emphasis>
</taxonomicName>
species phylogenetically (Fig.
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Phylogram of Diaporthe arecae species complex resulting from a maximum likelihood analysis based on a combined matrix of ITS, cal, his 3, tef 1 and tub 2 loci. Numbers above the branches indicate ML bootstrap values (left, ML BS ≥ 50 %) and Bayesian posterior probabilities (right, BPP ≥ 0.9). Isolates from the present study are marked in bold blue." figureDoi="10.3897/mycokeys.95.98969.figure2" httpUri="https://binary.pensoft.net/fig/817928" pageId="0" pageNumber="209">2</figureCitation>
).
<taxonomicName class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe eres" order="Diaporthales" pageId="0" pageNumber="209" phylum="Ascomycota" rank="species" species="eres">
<emphasis italics="true" pageId="0" pageNumber="209">Diaporthe eres</emphasis>
</taxonomicName>
(=
<taxonomicName lsidName="D. mahothocarpi" pageId="0" pageNumber="209" rank="species" species="mahothocarpi">
<emphasis italics="true" pageId="0" pageNumber="209">D. mahothocarpi</emphasis>
</taxonomicName>
),
<taxonomicName lsidName="D. lithocarpi" pageId="0" pageNumber="209" rank="species" species="lithocarpi">
<emphasis italics="true" pageId="0" pageNumber="209">D. lithocarpi</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="D. smilacicola" pageId="0" pageNumber="209" rank="species" species="smilacicola">
<emphasis italics="true" pageId="0" pageNumber="209">D. smilacicola</emphasis>
</taxonomicName>
have been reported from the host
<taxonomicName lsidName="S. glabra" pageId="0" pageNumber="209" rank="species" species="glabra">
<emphasis italics="true" pageId="0" pageNumber="209">S. glabra</emphasis>
</taxonomicName>
(
<bibRefCitation DOI="https://doi.org/10.1007/s11557-013-0898-2" author="Gao, YH" journalOrPublisher="Mycological Progress" pageId="0" pageNumber="209" pagination="111 - 121" refId="B17" refString="Gao, YH, Sun, W, Su, YY, Cai, L, 2013. Three new species of Phomopsis in Gutianshan nature reserve in China. Mycological Progress 13 (1): 111 - 121, DOI: https://doi.org/10.1007/s11557-013-0898-2" title="Three new species of Phomopsis in Gutianshan nature reserve in China." url="https://doi.org/10.1007/s11557-013-0898-2" volume="13" year="2013">Gao et al. 2013</bibRefCitation>
;
<bibRefCitation DOI="https://doi.org/10.3390/biology10030179" author="Chaisiri, C" journalOrPublisher="Biology (Basel)" pageId="0" pageNumber="209" pagination=": 179" refId="B5" refString="Chaisiri, C, Liu, X, Lin, Y, Fu, Y, Zhu, F, Luo, C, 2021. Phylogenetic and haplotype network analyses of Diaporthe eres species in China based on sequences of multiple loci. Biology (Basel) 10 (3): 179, DOI: https://doi.org/10.3390/biology10030179" title="Phylogenetic and haplotype network analyses of Diaporthe eres species in China based on sequences of multiple loci." url="https://doi.org/10.3390/biology10030179" volume="10" year="2021">Chaisiri et al. 2021</bibRefCitation>
). Morphologically, these three species are similar in conidial shape and size. However,
<taxonomicName class="Ascomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe eres" order="Diaporthales" pageId="0" pageNumber="209" phylum="Ascomycota" rank="species" species="eres">
<emphasis italics="true" pageId="0" pageNumber="209">Diaporthe eres</emphasis>
</taxonomicName>
belongs to
<taxonomicName lsidName="D. eres" pageId="0" pageNumber="209" rank="species" species="eres">
<emphasis italics="true" pageId="0" pageNumber="209">D. eres</emphasis>
</taxonomicName>
species complex, which is different from
<taxonomicName lsidName="D. lithocarpi" pageId="0" pageNumber="209" rank="species" species="lithocarpi">
<emphasis italics="true" pageId="0" pageNumber="209">D. lithocarpi</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="D. smilacicola" pageId="0" pageNumber="209" rank="species" species="smilacicola">
<emphasis italics="true" pageId="0" pageNumber="209">D. smilacicola</emphasis>
</taxonomicName>
in
<taxonomicName lsidName="D. arecae" pageId="0" pageNumber="209" rank="species" species="arecae">
<emphasis italics="true" pageId="0" pageNumber="209">D. arecae</emphasis>
</taxonomicName>
species complex.
<taxonomicName lsidName="D. smilacicola" pageId="0" pageNumber="209" rank="species" species="smilacicola">
<emphasis italics="true" pageId="0" pageNumber="209">D. smilacicola</emphasis>
</taxonomicName>
is obviously different from
<taxonomicName lsidName="D. lithocarpi" pageId="0" pageNumber="209" rank="species" species="lithocarpi">
<emphasis italics="true" pageId="0" pageNumber="209">D. lithocarpi</emphasis>
</taxonomicName>
based on sequence data (22/467 in ITS, 31/393 in
<emphasis italics="true" pageId="0" pageNumber="209">cal</emphasis>
, 52/317 in
<emphasis italics="true" pageId="0" pageNumber="209">tef1</emphasis>
, 19/420 in
<emphasis italics="true" pageId="0" pageNumber="209">tub2</emphasis>
) (Fig.
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Phylogram of Diaporthe arecae species complex resulting from a maximum likelihood analysis based on a combined matrix of ITS, cal, his 3, tef 1 and tub 2 loci. Numbers above the branches indicate ML bootstrap values (left, ML BS ≥ 50 %) and Bayesian posterior probabilities (right, BPP ≥ 0.9). Isolates from the present study are marked in bold blue." figureDoi="10.3897/mycokeys.95.98969.figure2" httpUri="https://binary.pensoft.net/fig/817928" pageId="0" pageNumber="209">2</figureCitation>
).
</paragraph>
</subSubSection>
</treatment>
</subSection>
</document>