361 lines
67 KiB
XML
361 lines
67 KiB
XML
<document id="52CD2B46CBD993C4F896C721E78DC188" ID-CLB-Dataset="7657" ID-DOI="10.1093/zoolinnean/zlz122" ID-GBIF-Dataset="ce6eb74d-6590-47d5-ba3a-a730db9a3baa" ID-Zenodo-Dep="4623663" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1616170722525" checkinUser="valdenar" docAuthor="Møbjerg, Nadja, Jørgensen, Aslak & Kristensen, Reinhardt M." docDate="2020" docId="AA47465C3D3AFF8429BCFAADFAF458D0" docLanguage="en" docName="ZoolJLinnSoc.188.3.663-680.pdf" docOrigin="Zoological Journal of the Linnean Society 188 (3)" docStyle="DocumentStyle:36B3BD6A90C22AB4F7F465C853188CC8.7:ZoolJLinnSoc.2017-2023.journal_article" docStyleId="36B3BD6A90C22AB4F7F465C853188CC8" docStyleName="ZoolJLinnSoc.2017-2023.journal_article" docStyleVersion="7" docTitle="Neoechiniscoides aski MOBJERG & JORGENSEN & KRISTENSEN 2020, SP. NOV." docType="treatment" docVersion="11" lastPageNumber="671" masterDocId="567E3E243D3CFF8C2921FFF4FFF35943" masterDocTitle="Ongoing revision of Echiniscoididae (Heterotardigrada: Echiniscoidea), with the description of a new interstitial species and genus with unique anal structures" masterLastPageNumber="680" masterPageNumber="663" pageNumber="669" updateTime="1698934381141" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="ECCE9E62095CE528A9D9CB85FC1B3957">Ongoing revision of Echiniscoididae (Heterotardigrada: Echiniscoidea), with the description of a new interstitial species and genus with unique anal structures</mods:title>
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<mods:name id="36AB811BAE54BC5CFD181B5161D6E92B" type="personal">
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<mods:roleTerm id="AB285F77FA12A499FA83129B44CF4077">Author</mods:roleTerm>
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<mods:namePart id="18DD75649F36506AD088F40C5666F005">Møbjerg, Nadja</mods:namePart>
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<mods:affiliation id="4EA17BE546B22670AA8AC64D3A741DA9">Department of Biology, August Krogh Building, University of Copenhagen, Denmark</mods:affiliation>
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<mods:nameIdentifier id="A5DC84DD88CB3DCBAC4F18FF017A7FE2" type="email">nmobjerg@bio.ku.dk</mods:nameIdentifier>
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<mods:namePart id="4087CF4F4B0E58A1C00B3D26839F6CF7">Jørgensen, Aslak</mods:namePart>
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<mods:affiliation id="240B4850C54D5AEB1D04E600A36E8307">Department of Biology, August Krogh Building, University of Copenhagen, Denmark</mods:affiliation>
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<mods:namePart id="45184D38D732F7F378D57BE4D4E82095">Kristensen, Reinhardt M.</mods:namePart>
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<mods:affiliation id="6AEAE3FB28D956D8E157ABF30B49CEB3">The Natural History Museum of Denmark, University of Copenhagen, Denmark</mods:affiliation>
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<mods:typeOfResource id="76B6CA52CF546B206695E3BA5D2661C4">text</mods:typeOfResource>
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<mods:title id="0E5F7D3DAD5DA24A3185DD79E9934C5B">Zoological Journal of the Linnean Society</mods:title>
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<mods:part id="7D6CFC712F4B2C19F7F13ED89F7B0CF9">
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<mods:date id="5FF0C39AFCF37E0C366EE90F8CA9D5D1">2020</mods:date>
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<mods:number id="43F77271F50ADF6340762AB6916BA466">2019-09-18</mods:number>
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<mods:number id="AADFD7CDA15107523E62B1DF8B948F42">188</mods:number>
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<mods:start id="6070F5F92B68662491DAC9AD57C07B76">663</mods:start>
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<mods:end id="36DC0865EB391C262BAA03C64CA0C595">680</mods:end>
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<treatment id="AA47465C3D3AFF8429BCFAADFAF458D0" ID-DOI="http://doi.org/10.5281/zenodo.4623691" ID-GBIF-Taxon="180598448" ID-Zenodo-Dep="4623691" LSID="urn:lsid:plazi:treatment:AA47465C3D3AFF8429BCFAADFAF458D0" httpUri="http://treatment.plazi.org/id/AA47465C3D3AFF8429BCFAADFAF458D0" lastPageId="8" lastPageNumber="671" pageId="6" pageNumber="669">
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<subSubSection id="6AF4A4C13D3AFF8A29BCFAADFDB15CCC" pageId="6" pageNumber="669" type="nomenclature">
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<paragraph id="2251F74A3D3AFF8A29BCFAADFDB15CCC" blockId="6.[145,762,1369,1892]" pageId="6" pageNumber="669">
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<taxonomicName id="E5EE8CC93D3AFF8A29BCFAADFE245CCC" ID-CoL="76547746-23ea-49c9-b411-127f67724c29" authority="MOBJERG & JORGENSEN & KRISTENSEN, 2020" authorityName="MOBJERG & JORGENSEN & KRISTENSEN" authorityYear="2020" class="Heterotardigrada" family="Echiniscoididae" genus="Neoechiniscoides" higherTaxonomySource="GBIF" kingdom="Animalia" order="Echiniscoidea" pageId="6" pageNumber="669" phylum="Tardigrada" rank="species" species="aski" status="sp. nov.">
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<heading id="791940263D3AFF8A29BCFAADFD1E5C31" allCaps="true" box="[157,749,1369,1394]" centered="true" fontSize="9" level="2" pageId="6" pageNumber="669" reason="5">
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<emphasis id="109A2B583D3AFF8A29BCFAADFE345C2C" bold="true" box="[157,455,1369,1392]" italics="true" pageId="6" pageNumber="669">NEOECHINISCOIDES ASKI</emphasis>
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MØBJERG, JØRGENSEN &
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</heading>
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<heading id="791940263D3AFF8A2869FA83FE245CCC" allCaps="true" box="[328,471,1399,1424]" centered="true" fontSize="9" level="2" pageId="6" pageNumber="669" reason="4">KRISTENSEN</heading>
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</taxonomicName>
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<emphasis id="109A2B583D3AFF8A28FEFA8FFDB15CCC" bold="true" box="[479,578,1399,1423]" pageId="6" pageNumber="669">
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<taxonomicNameLabel id="0BA996233D3AFF8A28FEFA8FFDB15CCC" box="[479,578,1399,1423]" pageId="6" pageNumber="669" rank="species">SP. NOV.</taxonomicNameLabel>
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</emphasis>
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</paragraph>
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</subSubSection>
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<subSubSection id="6AF4A4C13D3AFF8A29B0FA54FB865B2A" pageId="6" pageNumber="669" type="diagnosis">
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<paragraph id="2251F74A3D3AFF8A29B0FA54FB865B2A" blockId="6.[145,762,1369,1892]" lastBlockId="6.[809,1425,197,618]" pageId="6" pageNumber="669">
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<emphasis id="109A2B583D3AFF8A29B0FA54FEFB5CF4" box="[145,264,1440,1463]" italics="true" pageId="6" pageNumber="669">Diagnosis</emphasis>
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: Interstitial, medium-sized
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<taxonomicName id="E5EE8CC93D3AFF8A2B1FFA55FD0A5CF5" authorityName="ASKI MOBJERG, JORGENSEN & KRISTENSEN" authorityYear="2020" box="[574,761,1441,1462]" class="Heterotardigrada" family="Echiniscoididae" genus="Neoechiniscoides" higherTaxonomySource="GBIF" kingdom="Animalia" order="Echiniscoidea" pageId="6" pageNumber="669" phylum="Tardigrada" rank="genus">
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<emphasis id="109A2B583D3AFF8A2B1FFA55FD0A5CF5" box="[574,761,1441,1462]" italics="true" pageId="6" pageNumber="669">Neoechiniscoides</emphasis>
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</taxonomicName>
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with six to nine claws on each leg and 8,8,8,7 as the most common claw formula. Large black to dark brown eyes. Dorsal cuticle with star-like, polygonal structures arranged in transverse rows that continue ventrally on the head. Abdomen with a smooth cuticle, but polygonal structures are present on lateral anal lobes.The mouth is subterminaland lacksdorsalmouthplates; instead, three circular structures are present surrounding the mouth opening. The stylets and buccal tube are relatively short. Stylet supports are not observed using light microscopy. Calcium carbonate incrustations are found only in the anterior part of the pharyngeal placoids. Cirrus Aand E have a single spiral sculpture at the basis. Internal and external cirri have swollen bases. Spine-shaped setae are present on leg III. Legs Iand II have small domeshaped sensory organs, and leg IV has a papilla similar to the primary clava. Females have a flat and males a more raised, nearly papillary secondary clava. The male gonopore is a five-lobed, nearly penis-like structure (not ovoid). The female gonopore is a six-lobed rosette located on a ring-shaped protrusion. Asmall ventral plate is present in front of the female gonopore, and two small depressions (interpreted as muscle attachment sites) are present lateral to the female gonopore. Males lack this ventral plate; the muscle attachments are present, but placed more anteriorly with respect to the male gonopore. Both sexes have enlarged lateral anal lobes (neochiniscoidid anal system).
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</paragraph>
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||
</subSubSection>
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<subSubSection id="6AF4A4C13D3AFF8A2A08FD6EFBFE5BAC" pageId="6" pageNumber="669" type="etymology">
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<paragraph id="2251F74A3D3AFF8A2A08FD6EFBFE5BAC" blockId="6.[809,1425,666,751]" pageId="6" pageNumber="669">
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<emphasis id="109A2B583D3AFF8A2A08FD6EFC5E5BF1" box="[809,941,666,690]" italics="true" pageId="6" pageNumber="669">Etymology</emphasis>
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: Named for Ask Møbjerg Jørgensen, who in turn is named for the Nordic mythological ‘Tree of Life’, Ask Yggdrasil.
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</paragraph>
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</subSubSection>
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<subSubSection id="6AF4A4C13D3AFF842A08FCEBFD605816" lastPageId="8" lastPageNumber="671" pageId="6" pageNumber="669" type="materials_examined">
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<paragraph id="2251F74A3D3AFF8A2A08FCEBFB6B5AF4" blockId="6.[809,1425,799,951]" pageId="6" pageNumber="669">
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<materialsCitation id="9286FD173D3AFF8A2A08FCEBFB675AF4" ID-GBIF-Occurrence="3057268306" collectionCode="D" country="Denmark" pageId="6" pageNumber="669" specimenCode="NHMD-230293" specimenCount="1" specimenCount-female="1" typeStatus="holotype">
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<emphasis id="109A2B583D3AFF8A2A08FCEBFC685A74" box="[809,923,799,823]" italics="true" pageId="6" pageNumber="669">
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<typeStatus id="FD5549E83D3AFF8A2A08FCEBFC685A74" box="[809,923,799,823]" pageId="6" pageNumber="669" type="holotype">Holotype</typeStatus>
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</emphasis>
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(
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<figureCitation id="BAD5EBCF3D3AFF8A2A8FFCD5FBD85A74" box="[942,1067,801,823]" captionStart="Figure 2" captionStartId="7.[163,241,1675,1697]" captionTargetBox="[265,1371,158,1636]" captionTargetId="figure-26@7.[631,1340,510,914]" captionTargetPageId="7" captionText="Figure 2. Light microscopy of Neoechiniscoides aski. A–C, images of live, moving specimens. A, ventral view of a live adult female, showing the anal system with lateral wings. In live animals, the conspicuous anal complex moves from side to side when the animal walks/runs. Scale bar: 50 µm. B, dorsal view of live adult female used for DNA extraction (28S GenBank accession number KX363645; COI GenBank accession number KX363656). Note the eight to nine mature oocytes. Scale bar: 50 µm. C, dorsal view of live adult male. Scale bar: 50 µm. D, holotype of N. aski (female). Scale bar: 50 µm. E, paratypic allotype of N. aski (male). Scale bar: 50 µm. F, ventrocaudal view of holotypic female showing anal system and the gonopore, which is raised on an elevation (note that seminal receptacles are lacking). Scale bar: 20 µm. G, ventral view of paratypic" figureDoi="http://doi.org/10.5281/zenodo.4623673" httpUri="https://zenodo.org/record/4623673/files/figure.png" pageId="6" pageNumber="669">Figs 2D, F</figureCitation>
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,
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<figureCitation id="BAD5EBCF3D3AFF8A2D1DFCD5FBB95A75" box="[1084,1098,801,822]" captionStart="Figure 4" captionStartId="12.[145,223,1678,1700]" captionTargetBox="[380,1189,196,1637]" captionTargetId="figure-25@12.[379,1191,195,1638]" captionTargetPageId="12" captionText="Figure 4. Neoechiniscoides aski.Drawing of holotypicfemale (NHMD-230293),ventral view.Scale bar:50 µm.Abbreviations: an, anus; bt, buccal tube; cE, cirrus E; cm, claw membrane; ec, external cirrus; ey, eye; go, gonopore; ic, internal cirrus; lc, lateral cirrus (cirrus A); ma, lateral attachment sites associated with the female gonopore; mg, midgut; nc, new claws forming inside claw gland; oo, oocyte; ov, ovary; p1–p3, sensory structures of legs I–III, respectively; pa, ventral cuticular plate; pb, pharyngeal bulb; pc, primary clava; pl, placoid; sc, secondary clava; wl, lateral wing of the anal structure." figureDoi="http://doi.org/10.5281/zenodo.4623679" httpUri="https://zenodo.org/record/4623679/files/figure.png" pageId="6" pageNumber="669">4</figureCitation>
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;
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<tableCitation id="6F6CC2F13D3AFF8A2D78FCD5FB415A74" box="[1113,1202,801,823]" captionStart="Table 1" captionStartId="9.[163,227,196,217]" captionTargetPageId="9" captionText="Table 1. Measurements of selected characters on holotypes and paratypes of Neoechiniscoides aski and Neoechiniscoides pollocki" httpUri="http://table.plazi.org/id/7691A7C23D35FF852982FF30FF0759B4" pageId="6" pageNumber="669" tableUuid="7691A7C23D35FF852982FF30FF0759B4">Table 1</tableCitation>
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):
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<specimenCount id="34E83CC33D3AFF8A2DEFFCD5FADB5A74" box="[1230,1320,801,823]" pageId="6" pageNumber="669" type="female">Female</specimenCount>
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,
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<quantity id="E5165AAF3D3AFF8A2C17FCD5FA625A74" box="[1334,1425,800,823]" metricMagnitude="-1" metricUnit="m" metricValue="3.01" pageId="6" pageNumber="669" unit="mm" value="301.0">301 µm</quantity>
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long, with mature ovary (three oocytes) and welldeveloped six-lobed gonopore. The animal is in moult. Deposited at The Natural History Museum of Denmark (
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<specimenCode id="72485F313D3AFF8A2AEBFC6BFB785AF4" box="[970,1163,927,951]" pageId="6" pageNumber="669">NHMD-230293</specimenCode>
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)
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</materialsCitation>
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.
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</paragraph>
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<paragraph id="2251F74A3D3AFF8A2A08FC1CFA9E5D18" blockId="6.[809,1426,999,1115]" pageId="6" pageNumber="669">
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<materialsCitation id="9286FD173D3AFF8A2A08FC1CFA9A5D18" ID-GBIF-Occurrence="3057268308" collectedFrom="from oxic, sandy sediment with few stones" collectingDate="2013-07-17" collectionCode="N" country="Denmark" latitude="2.083611" location="L'Aber d e R o s c o f f, B r i t t a n y, Fr a n c e" longitude="-4.007367" pageId="6" pageNumber="669" specimenCount="1" typeStatus="type">
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<emphasis id="109A2B583D3AFF8A2A08FC1CFC3F5ABC" box="[809,972,999,1023]" italics="true" pageId="6" pageNumber="669">
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<typeStatus id="FD5549E83D3AFF8A2A08FC1CFC975ABC" box="[809,868,1000,1023]" pageId="6" pageNumber="669">Type</typeStatus>
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locality
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</emphasis>
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: Collected
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<date id="5650D18A3D3AFF8A2D73FC1DFB185ABD" box="[1106,1259,1001,1023]" pageId="6" pageNumber="669" value="2013-07-17">
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<collectingDate id="461428623D3AFF8A2D73FC1DFB185ABD" box="[1106,1259,1001,1023]" pageId="6" pageNumber="669" value="2013-07-17">
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17 July
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<quantity id="E5165AAF3D3AFF8A2D92FC1DFB185ABD" box="[1203,1259,1001,1022]" metricMagnitude="1" metricUnit="m" metricValue="5.11302" pageId="6" pageNumber="669" unit="in" value="2013.0">2013</quantity>
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</collectingDate>
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</date>
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in the
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<location id="2731A1913D3AFF8A2C65FC1DFB555D5E" LSID="urn:lsid:plazi:treatment:AA47465C3D3AFF8429BCFAADFAF458D0:2731A1913D3AFF8A2C65FC1DFB555D5E" country="Denmark" latitude="2.083611" longitude="-4.007367" name="L'Aber d e R o s c o f f, B r i t t a n y, Fr a n c e" pageId="6" pageNumber="669">L’Aber de Roscoff, Brittany, France</location>
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(
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<geoCoordinate id="47DA918D3D3AFF8A2D9EFBFCFA7E5D5E" box="[1215,1421,1031,1054]" pageId="6" pageNumber="669">48°43′17.251″N</geoCoordinate>
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,
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<geoCoordinate id="47DA918D3D3AFF8A2A08FBD3FC2A5D7F" box="[809,985,1061,1084]" degrees="04" direction="west" minutes="00" orientation="longitude" pageId="6" pageNumber="669" precision="1" seconds="26.522" value="-4.007367">04°00′26.522″W</geoCoordinate>
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) by Nadja Møbjergand Aslak Jørgensen. Collected
|
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<collectedFrom id="91F2B4B73D3AFF8A2AB4FBB1FA9A5D18" box="[917,1385,1093,1115]" pageId="6" pageNumber="669">from oxic, sandy sediment with few stones</collectedFrom>
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</materialsCitation>
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.
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</paragraph>
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<paragraph id="2251F74A3D3AFF8A2A08FB7FFBA35CDB" blockId="6.[809,1425,1163,1432]" pageId="6" pageNumber="669">
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<emphasis id="109A2B583D3AFF8A2A08FB7FFBFB5DE0" box="[809,1032,1163,1187]" italics="true" pageId="6" pageNumber="669">Allotypic paratype</emphasis>
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||
(
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<figureCitation id="BAD5EBCF3D3AFF8A2D37FB79FB9E5DE1" box="[1046,1133,1165,1187]" captionStart="Figure 2" captionStartId="7.[163,241,1675,1697]" captionTargetBox="[265,1371,158,1636]" captionTargetId="figure-26@7.[631,1340,510,914]" captionTargetPageId="7" captionText="Figure 2. Light microscopy of Neoechiniscoides aski. A–C, images of live, moving specimens. A, ventral view of a live adult female, showing the anal system with lateral wings. In live animals, the conspicuous anal complex moves from side to side when the animal walks/runs. Scale bar: 50 µm. B, dorsal view of live adult female used for DNA extraction (28S GenBank accession number KX363645; COI GenBank accession number KX363656). Note the eight to nine mature oocytes. Scale bar: 50 µm. C, dorsal view of live adult male. Scale bar: 50 µm. D, holotype of N. aski (female). Scale bar: 50 µm. E, paratypic allotype of N. aski (male). Scale bar: 50 µm. F, ventrocaudal view of holotypic female showing anal system and the gonopore, which is raised on an elevation (note that seminal receptacles are lacking). Scale bar: 20 µm. G, ventral view of paratypic" figureDoi="http://doi.org/10.5281/zenodo.4623673" httpUri="https://zenodo.org/record/4623673/files/figure.png" pageId="6" pageNumber="669">Figs 2E</figureCitation>
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,
|
||
<figureCitation id="BAD5EBCF3D3AFF8A2D58FB79FB745DE0" box="[1145,1159,1165,1187]" captionStart="Figure 5" captionStartId="13.[163,241,1678,1700]" captionTargetBox="[427,1185,205,1632]" captionTargetId="figure-26@13.[420,1185,195,1638]" captionTargetPageId="13" captionText="Figure 5. Neoechiniscoides aski. Drawing of allotypic male (NHMD-230297), dorsal view. Scale bar: 50 µm. Abbreviations: as, anal system; cE, cirrus E; cl, claw; ds, dorsal cuticular attachment sites; ec, external cirrus; fu, furca of the stylet; ic, internal cirrus; lc, lateral cirrus (cirrus A); mg, midgut; p1–p4, sensory structures of legs I–IV, respectively; pc, primary clava; sc, secondary clava; sp, spermatozoa; sv, seminal vesicle; te, testicle." figureDoi="http://doi.org/10.5281/zenodo.4623683" httpUri="https://zenodo.org/record/4623683/files/figure.png" pageId="6" pageNumber="669">5</figureCitation>
|
||
;
|
||
<tableCitation id="6F6CC2F13D3AFF8A2DB3FB79FB165DE1" box="[1170,1253,1165,1187]" captionStart="Table 1" captionStartId="9.[163,227,196,217]" captionTargetPageId="9" captionText="Table 1. Measurements of selected characters on holotypes and paratypes of Neoechiniscoides aski and Neoechiniscoides pollocki" httpUri="http://table.plazi.org/id/7691A7C23D35FF852982FF30FF0759B4" pageId="6" pageNumber="669" tableUuid="7691A7C23D35FF852982FF30FF0759B4">Table 1</tableCitation>
|
||
<emphasis id="109A2B583D3AFF8A2DC5FB79FB005DE1" box="[1252,1267,1165,1186]" italics="true" pageId="6" pageNumber="669">):</emphasis>
|
||
<materialsCitation id="9286FD173D3AFF8A2DDBFB79FBBF5CDB" ID-GBIF-Occurrence="3057268304" collectedFrom="oxic, sandy sediment with few stones" collectingDate="2012-07-06" collectionCode="E" collectorName="Nadja Mobjerg & Aslak Jorgensen" country="France" latitude="48.72146" location="L'Aber de Roscoff" longLatPrecision="1" longitude="-4.007367" pageId="6" pageNumber="669" specimenCode="NHMD-230297" specimenCount="1" specimenCount-male="1" typeStatus="paratype">
|
||
<specimenCount id="34E83CC33D3AFF8A2DDBFB79FAC65DE0" box="[1274,1333,1165,1187]" pageId="6" pageNumber="669" type="male">Male</specimenCount>
|
||
,
|
||
<quantity id="E5165AAF3D3AFF8A2C1FFB79FA625DE0" box="[1342,1425,1165,1187]" metricMagnitude="-1" metricUnit="m" metricValue="2.28" pageId="6" pageNumber="669" unit="mm" value="228.0">228 µm</quantity>
|
||
long, with large secondary clavae, mature spermatozoa in the seminal vesicles and well-developed papillary gonopore. Deposited at The Natural History Museum of Denmark (
|
||
<specimenCode id="72485F313D3AFF8A2D1BFAFCFB055C5D" box="[1082,1270,1288,1310]" pageId="6" pageNumber="669">NHMD-230297</specimenCode>
|
||
). Collected
|
||
<date id="5650D18A3D3AFF8A2CA2FAFCFC6F5C7F" pageId="6" pageNumber="669" value="2012-07-06">
|
||
<collectingDate id="461428623D3AFF8A2CA2FAFCFC6F5C7F" pageId="6" pageNumber="669" value="2012-07-06">
|
||
6 July
|
||
<quantity id="E5165AAF3D3AFF8A2A45FAD3FC6F5C7F" box="[868,924,1319,1340]" metricMagnitude="1" metricUnit="m" metricValue="5.11048" pageId="6" pageNumber="669" unit="in" value="2012.0">2012</quantity>
|
||
</collectingDate>
|
||
</date>
|
||
in the
|
||
<location id="2731A1913D3AFF8A2AD2FAD2FB315C7F" LSID="urn:lsid:plazi:treatment:AA47465C3D3AFF8429BCFAADFAF458D0:2731A1913D3AFF8A2AD2FAD2FB315C7F" box="[1011,1218,1318,1340]" country="France" latitude="48.72146" longLatPrecision="1" longitude="-4.007367" name="L'Aber de Roscoff" pageId="6" pageNumber="669">L’Aber de Roscoff</location>
|
||
,
|
||
<location id="2731A1913D3AFF8A2DEFFAD2FAC15C7F" LSID="urn:lsid:plazi:treatment:AA47465C3D3AFF8429BCFAADFAF458D0:2731A1913D3AFF8A2DEFFAD2FAC15C7F" box="[1230,1330,1318,1340]" country="France" latitude="48.72146" longLatPrecision="1" longitude="-4.007367" name="Brittany" pageId="6" pageNumber="669">Brittany</location>
|
||
,
|
||
<collectingCountry id="5AF9B7DA3D3AFF8A2C1EFAD3FA635C7F" box="[1343,1424,1319,1340]" name="France" pageId="6" pageNumber="669" value="France">France</collectingCountry>
|
||
(
|
||
<geoCoordinate id="47DA918D3D3AFF8A2A10FAB1FC1B5C18" box="[817,1000,1348,1371]" degrees="48" direction="north" minutes="43" orientation="latitude" pageId="6" pageNumber="669" precision="1" seconds="17.251" value="48.72146">48°43′17.251″N</geoCoordinate>
|
||
,
|
||
<geoCoordinate id="47DA918D3D3AFF8A2AD5FAB1FB435C18" box="[1012,1200,1348,1371]" degrees="04" direction="west" minutes="00" orientation="longitude" pageId="6" pageNumber="669" precision="1" seconds="26.522" value="-4.007367">04°00′26.522″W</geoCoordinate>
|
||
) by
|
||
<collectorName id="8F1B929C3D3AFF8A2DC3FAB1FA635C18" box="[1250,1424,1349,1371]" pageId="6" pageNumber="669">Nadja Møbjerg</collectorName>
|
||
and
|
||
<collectorName id="8F1B929C3D3AFF8A2A40FA90FBC75C3A" box="[865,1076,1380,1402]" pageId="6" pageNumber="669">Aslak Jørgensen</collectorName>
|
||
. Collected from
|
||
<collectedFrom id="91F2B4B73D3AFF8A2C27FA90FBBF5CDB" pageId="6" pageNumber="669">oxic, sandy sediment with few stones</collectedFrom>
|
||
</materialsCitation>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="2251F74A3D3AFF842A08FA4AFD605816" blockId="6.[809,1426,1470,1892]" lastBlockId="8.[145,761,197,342]" lastPageId="8" lastPageNumber="671" pageId="6" pageNumber="669">
|
||
<emphasis id="109A2B583D3AFF8A2A08FA4AFC1F5C95" box="[809,1004,1470,1494]" italics="true" pageId="6" pageNumber="669">Other paratypes</emphasis>
|
||
(
|
||
<figureCitation id="BAD5EBCF3D3AFF8A2ADAFA34FB585C96" box="[1019,1195,1472,1494]" captionStart="Figure 2" captionStartId="7.[163,241,1675,1697]" captionTargetBox="[265,1371,158,1636]" captionTargetId="figure-26@7.[631,1340,510,914]" captionTargetPageId="7" captionText="Figure 2. Light microscopy of Neoechiniscoides aski. A–C, images of live, moving specimens. A, ventral view of a live adult female, showing the anal system with lateral wings. In live animals, the conspicuous anal complex moves from side to side when the animal walks/runs. Scale bar: 50 µm. B, dorsal view of live adult female used for DNA extraction (28S GenBank accession number KX363645; COI GenBank accession number KX363656). Note the eight to nine mature oocytes. Scale bar: 50 µm. C, dorsal view of live adult male. Scale bar: 50 µm. D, holotype of N. aski (female). Scale bar: 50 µm. E, paratypic allotype of N. aski (male). Scale bar: 50 µm. F, ventrocaudal view of holotypic female showing anal system and the gonopore, which is raised on an elevation (note that seminal receptacles are lacking). Scale bar: 20 µm. G, ventral view of paratypic" figureDoi="http://doi.org/10.5281/zenodo.4623673" httpUri="https://zenodo.org/record/4623673/files/figure.png" pageId="6" pageNumber="669">Figs 2C, 2G, 2H</figureCitation>
|
||
,
|
||
<figureCitation id="BAD5EBCF3D3AFF8A2D96FA34FB365C96" box="[1207,1221,1472,1493]" captionStart="Figure 3" captionStartId="10.[145,225,1675,1697]" captionTargetBox="[199,1371,195,1635]" captionTargetId="figure-24@10.[199,1371,195,1635]" captionTargetPageId="10" captionText="Figure 3. Scanning electron microscopy of Neoechiniscoides aski. A–C, female. A, habitus of mature female. Scale bar: 10 µm. B, close-up of gonopore with associated cuticular plate and anus. Note muscle attachment sites located lateral to the female gonopore. Scale bar: 10 µm. C, close-up of head region. Note the cuticular sculpture of the head. Scale bar: 10 µm. D–H, male. D, second leg, with dome-shaped sense organ (p2). Scale bar: 1 µm. E, habitus of adult male. Scale bar: 10 µm. F, prominent dorsal cuticular attachment sites of the caudal segment. Scale bar: 1 µm. G, posterioventral part of a male, showing the fourth leg pair, anal system and gonopore. Anterior is to the left. Scale bar: 10 µm. H, close-up of head region of male. Note the relatively large secondary clavae. Scale bar: 1 µm. Abbreviations: an, anus; cE, cirrus E; cl, claw; ec, external" figureDoi="http://doi.org/10.5281/zenodo.4623677" httpUri="https://zenodo.org/record/4623677/files/figure.png" pageId="6" pageNumber="669">3</figureCitation>
|
||
;
|
||
<tableCitation id="6F6CC2F13D3AFF8A2DEEFA34FAD35C95" box="[1231,1312,1472,1494]" captionStart="Table 1" captionStartId="9.[163,227,196,217]" captionTargetPageId="9" captionText="Table 1. Measurements of selected characters on holotypes and paratypes of Neoechiniscoides aski and Neoechiniscoides pollocki" httpUri="http://table.plazi.org/id/7691A7C23D35FF852982FF30FF0759B4" pageId="6" pageNumber="669" tableUuid="7691A7C23D35FF852982FF30FF0759B4">Table 1</tableCitation>
|
||
):
|
||
<materialsCitation id="9286FD173D3AFF8A2C16FA34FB695E06" ID-GBIF-Occurrence="3057268303" collectingDate="2012-07-06" collectingDateMax="2013-07-17" collectingDateMin="2012-07-06" collectionCode="C" country="Denmark" pageId="6" pageNumber="669" specimenCode="NHMD-230294 to 230302, NHMD-230303 to 230305" specimenCount="16" typeStatus="paratype">
|
||
In total,
|
||
<specimenCount id="34E83CC33D3AFF8A2A08FA2BFC3B5CB7" box="[809,968,1502,1524]" pageId="6" pageNumber="669">14 additional</specimenCount>
|
||
paratypes were collected at the same locality as the holotype and allotype in the periodfrom
|
||
<date id="5650D18A3D3AFF8A2CA2FA09FC665F72" pageId="6" pageNumber="669" value="2012-07-06">
|
||
<collectingDate id="461428623D3AFF8A2CA2FA09FC665F72" pageId="6" pageNumber="669" value="2012-07-06">6 July 2012</collectingDate>
|
||
</date>
|
||
to
|
||
<date id="5650D18A3D3AFF8A2A95F9E8FBB15F72" box="[948,1090,1564,1586]" pageId="6" pageNumber="669" value="2013-07-17">
|
||
<collectingDate id="461428623D3AFF8A2A95F9E8FBB15F72" box="[948,1090,1564,1586]" pageId="6" pageNumber="669" value="2013-07-17">17 July 2013</collectingDate>
|
||
</date>
|
||
.
|
||
<specimenCount id="34E83CC33D3AFF8A2D6BF9E8FAFB5F71" box="[1098,1288,1564,1586]" pageId="6" pageNumber="669">Seven specimens</specimenCount>
|
||
(six females and one male) were mounted on microslides in glycerol and sealed with Glyceel (
|
||
<specimenCode id="72485F313D3AFF8A2D72F9ADFA755F2C" box="[1107,1414,1625,1647]" pageId="6" pageNumber="669">NHMD-230294 to 230302</specimenCode>
|
||
).
|
||
<specimenCount id="34E83CC33D3AFF8A2A08F98CFC145FCE" box="[809,999,1656,1677]" pageId="6" pageNumber="669">Seven paratypes</specimenCount>
|
||
(four females and three males) were mounted on three SEM-stubs (
|
||
<specimenCode id="72485F313D3AFF8A2D90F963FC8C5F88" pageId="6" pageNumber="669">NHMD-230303 to 230305</specimenCode>
|
||
) and used for scanning electron microscopy. All specimens were deposited at The Natural History Museum of Denmark. Additionally, digital images of four voucher specimens that have been used for molecular work exist (
|
||
<figureCitation id="BAD5EBCF3D3AFF8A2D63F8C4FB695E06" box="[1090,1178,1840,1862]" captionStart="Figure 2" captionStartId="7.[163,241,1675,1697]" captionTargetBox="[265,1371,158,1636]" captionTargetId="figure-26@7.[631,1340,510,914]" captionTargetPageId="7" captionText="Figure 2. Light microscopy of Neoechiniscoides aski. A–C, images of live, moving specimens. A, ventral view of a live adult female, showing the anal system with lateral wings. In live animals, the conspicuous anal complex moves from side to side when the animal walks/runs. Scale bar: 50 µm. B, dorsal view of live adult female used for DNA extraction (28S GenBank accession number KX363645; COI GenBank accession number KX363656). Note the eight to nine mature oocytes. Scale bar: 50 µm. C, dorsal view of live adult male. Scale bar: 50 µm. D, holotype of N. aski (female). Scale bar: 50 µm. E, paratypic allotype of N. aski (male). Scale bar: 50 µm. F, ventrocaudal view of holotypic female showing anal system and the gonopore, which is raised on an elevation (note that seminal receptacles are lacking). Scale bar: 20 µm. G, ventral view of paratypic" figureDoi="http://doi.org/10.5281/zenodo.4623673" httpUri="https://zenodo.org/record/4623673/files/figure.png" pageId="6" pageNumber="669">Fig. 2B</figureCitation>
|
||
</materialsCitation>
|
||
;
|
||
<materialsCitation id="9286FD173D3AFF842D8DF8C4FDB15998" ID-GBIF-Occurrence="3057268301" collectionCode="B" country="Denmark" lastPageId="8" lastPageNumber="671" pageId="6" pageNumber="669">
|
||
see
|
||
<bibRefCitation id="467F8ABB3D3AFF8A2DFCF8C4FC915E27" author="Mobjerg N & Kristensen RM & Jorgensen A." journalOrPublisher="Zoological Journal of the Linnean Society" pageId="6" pageNumber="669" pagination="804 - 814" part="178" refId="ref11359" refString="Mobjerg N, Kristensen RM, Jorgensen A. 2016. Data from new taxa infer Isoechiniscoides gen. nov. and increase the phylogenetic and evolutionary understanding of echiniscoidid tardigrades (Echiniscoidea: Tardigrada). Zoological Journal of the Linnean Society 178: 804 - 814." title="Data from new taxa infer Isoechiniscoides gen. nov. and increase the phylogenetic and evolutionary understanding of echiniscoidid tardigrades (Echiniscoidea: Tardigrada)" type="journal article" year="2016">
|
||
Møbjerg
|
||
<emphasis id="109A2B583D3AFF8A2C6AF8C4FA795E06" box="[1355,1418,1840,1861]" italics="true" pageId="6" pageNumber="669">et al.</emphasis>
|
||
, 2016
|
||
</bibRefCitation>
|
||
:
|
||
<taxonomicName id="E5EE8CC93D3AFF8A2A52F8BAFBEB5E20" authorityName="Plate" authorityYear="1889" box="[883,1048,1870,1891]" class="Heterotardigrada" family="Echiniscoididae" genus="Echiniscoides" kingdom="Animalia" order="Echiniscoidea" pageId="6" pageNumber="669" phylum="Tardigrada" rank="genus">
|
||
<emphasis id="109A2B583D3AFF8A2A52F8BAFBEB5E20" box="[883,1048,1870,1891]" italics="true" pageId="6" pageNumber="669">Echiniscoides</emphasis>
|
||
</taxonomicName>
|
||
sp.
|
||
<collectionCode id="44FF6F8F3D3AFF8A2D6DF8BAFBAC5E27" box="[1100,1119,1870,1892]" pageId="6" pageNumber="669">B</collectionCode>
|
||
/
|
||
<collectionCode id="44FF6F8F3D3AFF8A2D49F8BAFB885E27" box="[1128,1147,1870,1892]" pageId="6" pageNumber="669">T</collectionCode>
|
||
16, GenBank accession numbers
|
||
<accessionNumber id="3DBD6AA93D34FF842824FF31FE705998" box="[261,387,197,219]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KX363644" pageId="8" pageNumber="671">
|
||
<collectionCode id="44FF6F8F3D34FF842824FF31FEDD5998" box="[261,302,197,219]" pageId="8" pageNumber="671">KX</collectionCode>
|
||
363644
|
||
</accessionNumber>
|
||
and
|
||
<accessionNumber id="3DBD6AA93D34FF8428E5FF31FDB15998" box="[452,578,197,219]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KX363655" pageId="8" pageNumber="671">
|
||
<collectionCode id="44FF6F8F3D34FF8428E5FF31FE1E5998" box="[452,493,197,219]" pageId="8" pageNumber="671">KX</collectionCode>
|
||
363655
|
||
</accessionNumber>
|
||
</materialsCitation>
|
||
;
|
||
<materialsCitation id="9286FD173D34FF842B72FF31FEB7585B" ID-GBIF-Occurrence="3057268305" collectionCode="B" country="Denmark" pageId="8" pageNumber="671" specimenCount="1">
|
||
<taxonomicName id="E5EE8CC93D34FF842B72FF31FD0A5999" authorityName="Plate" authorityYear="1889" box="[595,761,197,218]" class="Heterotardigrada" family="Echiniscoididae" genus="Echiniscoides" kingdom="Animalia" order="Echiniscoidea" pageId="8" pageNumber="671" phylum="Tardigrada" rank="genus">
|
||
<emphasis id="109A2B583D34FF842B72FF31FD0A5999" box="[595,761,197,218]" italics="true" pageId="8" pageNumber="671">Echiniscoides</emphasis>
|
||
</taxonomicName>
|
||
sp. B/T18, GenBank accession numbers
|
||
<accessionNumber id="3DBD6AA93D34FF842B5DFF10FD0B59B9" box="[636,760,228,250]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KX363645" pageId="8" pageNumber="671">KX363645</accessionNumber>
|
||
and
|
||
<accessionNumber id="3DBD6AA93D34FF8429E6FEF6FEB7585B" box="[199,324,258,280]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KX363656" pageId="8" pageNumber="671">KX363656</accessionNumber>
|
||
</materialsCitation>
|
||
;
|
||
<materialsCitation id="9286FD173D34FF842874FEF6FE2A5874" ID-GBIF-Occurrence="3057268302" collectionCode="B" country="Denmark" pageId="8" pageNumber="671" specimenCount="1">
|
||
<taxonomicName id="E5EE8CC93D34FF842874FEF6FE095854" authorityName="Plate" authorityYear="1889" box="[341,506,258,279]" class="Heterotardigrada" family="Echiniscoididae" genus="Echiniscoides" kingdom="Animalia" order="Echiniscoidea" pageId="8" pageNumber="671" phylum="Tardigrada" rank="genus">
|
||
<emphasis id="109A2B583D34FF842874FEF6FE095854" box="[341,506,258,279]" italics="true" pageId="8" pageNumber="671">Echiniscoides</emphasis>
|
||
</taxonomicName>
|
||
sp. B/T20, GenBank accession number
|
||
<accessionNumber id="3DBD6AA93D34FF842843FED5FE2A5874" box="[354,473,289,311]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KX363657" pageId="8" pageNumber="671">KX363657</accessionNumber>
|
||
</materialsCitation>
|
||
;
|
||
<materialsCitation id="9286FD173D34FF8428C7FED5FD7D5816" ID-GBIF-Occurrence="3057268307" collectionCode="B" country="Denmark" pageId="8" pageNumber="671" specimenCount="1">
|
||
and
|
||
<taxonomicName id="E5EE8CC93D34FF842B39FED5FD415875" authorityName="Plate" authorityYear="1889" box="[536,690,289,310]" class="Heterotardigrada" family="Echiniscoididae" genus="Echiniscoides" kingdom="Animalia" order="Echiniscoidea" pageId="8" pageNumber="671" phylum="Tardigrada" rank="genus">
|
||
<emphasis id="109A2B583D34FF842B39FED5FD415875" box="[536,690,289,310]" italics="true" pageId="8" pageNumber="671">Echiniscoides</emphasis>
|
||
</taxonomicName>
|
||
sp. B/ T21, GenBank accession number
|
||
<accessionNumber id="3DBD6AA93D34FF842B2CFEB4FD705815" box="[525,643,320,342]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KX363658" pageId="8" pageNumber="671">KX363658</accessionNumber>
|
||
)
|
||
</materialsCitation>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<caption id="7691A7C23D3BFF8B2982F97FFA515E12" ID-DOI="http://doi.org/10.5281/zenodo.4623673" ID-Zenodo-Dep="4623673" httpUri="https://zenodo.org/record/4623673/files/figure.png" pageId="7" pageNumber="670" startId="7.[163,241,1675,1697]" targetBox="[265,1371,158,1636]" targetPageId="7">
|
||
<paragraph id="2251F74A3D3BFF8B2982F97FFA515E12" blockId="7.[163,1443,1675,1873]" pageId="7" pageNumber="670">
|
||
<emphasis id="109A2B583D3BFF8B2982F97FFEF85FE3" bold="true" box="[163,267,1675,1697]" pageId="7" pageNumber="670">Figure 2.</emphasis>
|
||
Light microscopy of
|
||
<emphasis id="109A2B583D3BFF8B28C9F97FFD235FE2" box="[488,720,1675,1697]" italics="true" pageId="7" pageNumber="670">
|
||
<taxonomicName id="E5EE8CC93D3BFF8B28C9F97FFD385FE2" authorityName="Møbjerg1 & Jørgensen & Kristensen" authorityYear="2020" box="[488,715,1675,1697]" class="Heterotardigrada" family="Echiniscoididae" genus="Neoechiniscoides" higherTaxonomySource="GBIF" kingdom="Animalia" order="Echiniscoidea" pageId="7" pageNumber="670" phylum="Tardigrada" rank="species" species="aski">Neoechiniscoides aski</taxonomicName>
|
||
.
|
||
</emphasis>
|
||
A–C, images of live, moving specimens. A, ventral view of a live adult female, showing the anal system with lateral wings. In live animals, the conspicuous anal complex moves from side to side when the animal walks/runs. Scale bar: 50 µm. B, dorsal view of live adult female used for DNA extraction (
|
||
<emphasis id="109A2B583D3BFF8B2C31F932FACB5F98" box="[1296,1336,1734,1755]" italics="true" pageId="7" pageNumber="670">28S</emphasis>
|
||
GenBank accession number
|
||
<accessionNumber id="3DBD6AA93D3BFF8B2846F917FE255FBA" box="[359,470,1763,1785]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KX363645" pageId="7" pageNumber="670">KX363645</accessionNumber>
|
||
;
|
||
<emphasis id="109A2B583D3BFF8B28C2F917FDFC5FBA" box="[483,527,1763,1785]" italics="true" pageId="7" pageNumber="670">COI</emphasis>
|
||
GenBank accession number
|
||
<accessionNumber id="3DBD6AA93D3BFF8B2A67F917FC465FBA" box="[838,949,1763,1785]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/KX363656" pageId="7" pageNumber="670">KX363656</accessionNumber>
|
||
). Note the eight to nine mature oocytes. Scale bar: 50 µm. C, dorsal view of live adult male. Scale bar: 50 µm. D, holotype of
|
||
<taxonomicName id="E5EE8CC93D3BFF8B2A9FF8F5FBF55E55" authorityName="Møbjerg1 & Jørgensen & Kristensen" authorityYear="2020" box="[958,1030,1792,1814]" class="Heterotardigrada" family="Echiniscoididae" genus="Neoechiniscoides" higherTaxonomySource="GBIF" kingdom="Animalia" order="Echiniscoidea" pageId="7" pageNumber="670" phylum="Tardigrada" rank="species" species="aski">
|
||
<emphasis id="109A2B583D3BFF8B2A9FF8F5FBF55E55" box="[958,1030,1792,1814]" italics="true" pageId="7" pageNumber="670">N. aski</emphasis>
|
||
</taxonomicName>
|
||
(female). Scale bar: 50 µm. E, paratypic allotype of
|
||
<taxonomicName id="E5EE8CC93D3BFF8B2834F8EAFEAF5E77" authorityName="Møbjerg1 & Jørgensen & Kristensen" authorityYear="2020" box="[277,348,1822,1844]" class="Heterotardigrada" family="Echiniscoididae" genus="Neoechiniscoides" higherTaxonomySource="GBIF" kingdom="Animalia" order="Echiniscoidea" pageId="7" pageNumber="670" phylum="Tardigrada" rank="species" species="aski">
|
||
<emphasis id="109A2B583D3BFF8B2834F8EAFEAF5E77" box="[277,348,1822,1844]" italics="true" pageId="7" pageNumber="670">N. aski</emphasis>
|
||
</taxonomicName>
|
||
(male). Scale bar: 50 µm. F, ventrocaudal view of holotypic female showing anal system and the gonopore, which is raised on an elevation (note that seminal receptacles are lacking). Scale bar: 20 µm. G, ventral view of paratypic
|
||
</paragraph>
|
||
</caption>
|
||
<subSubSection id="6AF4A4C13D34FF8429B0FE6BFAF458D0" pageId="8" pageNumber="671" type="description">
|
||
<paragraph id="2251F74A3D34FF8429B0FE6BFAF458D0" blockId="8.[145,762,415,1695]" lastBlockId="8.[809,1425,197,403]" pageId="8" pageNumber="671">
|
||
<emphasis id="109A2B583D34FF8429B0FE6BFEC358F5" box="[145,304,415,438]" italics="true" pageId="8" pageNumber="671">Description</emphasis>
|
||
(
|
||
<figureCitation id="BAD5EBCF3D34FF842869FE54FE4B58F5" box="[328,440,416,438]" captionStart-0="Figure 2" captionStart-1="Figure 3" captionStart-2="Figure 4" captionStart-3="Figure 5" captionStart-4="Figure 6" captionStartId-0="7.[163,241,1675,1697]" captionStartId-1="10.[145,225,1675,1697]" captionStartId-2="12.[145,223,1678,1700]" captionStartId-3="13.[163,241,1678,1700]" captionStartId-4="14.[146,225,1001,1023]" captionTargetBox-0="[265,1371,158,1636]" captionTargetBox-1="[199,1371,195,1635]" captionTargetBox-2="[380,1189,196,1637]" captionTargetBox-3="[427,1185,205,1632]" captionTargetBox-4="[370,1201,196,960]" captionTargetId-0="figure-26@7.[631,1340,510,914]" captionTargetId-1="figure-24@10.[199,1371,195,1635]" captionTargetId-2="figure-25@12.[379,1191,195,1638]" captionTargetId-3="figure-26@13.[420,1185,195,1638]" captionTargetId-4="figure-362@14.[369,1201,195,961]" captionTargetPageId-0="7" captionTargetPageId-1="10" captionTargetPageId-2="12" captionTargetPageId-3="13" captionTargetPageId-4="14" captionText-0="Figure 2. Light microscopy of Neoechiniscoides aski. A–C, images of live, moving specimens. A, ventral view of a live adult female, showing the anal system with lateral wings. In live animals, the conspicuous anal complex moves from side to side when the animal walks/runs. Scale bar: 50 µm. B, dorsal view of live adult female used for DNA extraction (28S GenBank accession number KX363645; COI GenBank accession number KX363656). Note the eight to nine mature oocytes. Scale bar: 50 µm. C, dorsal view of live adult male. Scale bar: 50 µm. D, holotype of N. aski (female). Scale bar: 50 µm. E, paratypic allotype of N. aski (male). Scale bar: 50 µm. F, ventrocaudal view of holotypic female showing anal system and the gonopore, which is raised on an elevation (note that seminal receptacles are lacking). Scale bar: 20 µm. G, ventral view of paratypic" captionText-1="Figure 3. Scanning electron microscopy of Neoechiniscoides aski. A–C, female. A, habitus of mature female. Scale bar: 10 µm. B, close-up of gonopore with associated cuticular plate and anus. Note muscle attachment sites located lateral to the female gonopore. Scale bar: 10 µm. C, close-up of head region. Note the cuticular sculpture of the head. Scale bar: 10 µm. D–H, male. D, second leg, with dome-shaped sense organ (p2). Scale bar: 1 µm. E, habitus of adult male. Scale bar: 10 µm. F, prominent dorsal cuticular attachment sites of the caudal segment. Scale bar: 1 µm. G, posterioventral part of a male, showing the fourth leg pair, anal system and gonopore. Anterior is to the left. Scale bar: 10 µm. H, close-up of head region of male. Note the relatively large secondary clavae. Scale bar: 1 µm. Abbreviations: an, anus; cE, cirrus E; cl, claw; ec, external" captionText-2="Figure 4. Neoechiniscoides aski.Drawing of holotypicfemale (NHMD-230293),ventral view.Scale bar:50 µm.Abbreviations: an, anus; bt, buccal tube; cE, cirrus E; cm, claw membrane; ec, external cirrus; ey, eye; go, gonopore; ic, internal cirrus; lc, lateral cirrus (cirrus A); ma, lateral attachment sites associated with the female gonopore; mg, midgut; nc, new claws forming inside claw gland; oo, oocyte; ov, ovary; p1–p3, sensory structures of legs I–III, respectively; pa, ventral cuticular plate; pb, pharyngeal bulb; pc, primary clava; pl, placoid; sc, secondary clava; wl, lateral wing of the anal structure." captionText-3="Figure 5. Neoechiniscoides aski. Drawing of allotypic male (NHMD-230297), dorsal view. Scale bar: 50 µm. Abbreviations: as, anal system; cE, cirrus E; cl, claw; ds, dorsal cuticular attachment sites; ec, external cirrus; fu, furca of the stylet; ic, internal cirrus; lc, lateral cirrus (cirrus A); mg, midgut; p1–p4, sensory structures of legs I–IV, respectively; pc, primary clava; sc, secondary clava; sp, spermatozoa; sv, seminal vesicle; te, testicle." captionText-4="Figure 6. Drawing of anal system in Isoechiniscoides, Echiniscoides and Neoechiniscoides based on investigations of live animals and preparations on slides for light microscopy. In all three genera, a three-lobed anal system is present and consists of two lateral lobes and a terminal lobe of variable size. The anal opening is indicated in black. In Neoechiniscoides, two wings are present on the lateral lobes. These wings are easily visualized in live animals and using light microscopic preparations, whereas this double structure of the lateral lobes is difficult to visualize in specimens prepared for scanning electron microscopy. Scale bar: 10 µm." figureDoi-0="http://doi.org/10.5281/zenodo.4623673" figureDoi-1="http://doi.org/10.5281/zenodo.4623677" figureDoi-2="http://doi.org/10.5281/zenodo.4623679" figureDoi-3="http://doi.org/10.5281/zenodo.4623683" figureDoi-4="http://doi.org/10.5281/zenodo.4623687" httpUri-0="https://zenodo.org/record/4623673/files/figure.png" httpUri-1="https://zenodo.org/record/4623677/files/figure.png" httpUri-2="https://zenodo.org/record/4623679/files/figure.png" httpUri-3="https://zenodo.org/record/4623683/files/figure.png" httpUri-4="https://zenodo.org/record/4623687/files/figure.png" pageId="8" pageNumber="671">Figs 2–6</figureCitation>
|
||
;
|
||
<tableCitation id="6F6CC2F13D34FF8428EDFE54FDD858F5" box="[460,555,416,438]" captionStart="Table 1" captionStartId="9.[163,227,196,217]" captionTargetPageId="9" captionText="Table 1. Measurements of selected characters on holotypes and paratypes of Neoechiniscoides aski and Neoechiniscoides pollocki" httpUri="http://table.plazi.org/id/7691A7C23D35FF852982FF30FF0759B4" pageId="8" pageNumber="671" tableUuid="7691A7C23D35FF852982FF30FF0759B4">Table 1</tableCitation>
|
||
): The collected specimens have a measured body length ranging from 207 (smallest males) to
|
||
<quantity id="E5165AAF3D34FF842896FE29FDFC58B0" box="[439,527,477,499]" metricMagnitude="-1" metricUnit="m" metricValue="3.01" pageId="8" pageNumber="671" unit="mm" value="301.0">301 µm</quantity>
|
||
(holotypic female). The most common claw formula of
|
||
<taxonomicName id="E5EE8CC93D34FF842B3EFE08FD9D5B52" authorityName="Møbjerg1 & Jørgensen & Kristensen" authorityYear="2020" box="[543,622,508,529]" class="Heterotardigrada" family="Echiniscoididae" genus="Neoechiniscoides" higherTaxonomySource="GBIF" kingdom="Animalia" order="Echiniscoidea" pageId="8" pageNumber="671" phylum="Tardigrada" rank="species" species="aski">
|
||
<emphasis id="109A2B583D34FF842B3EFE08FD9D5B52" box="[543,622,508,529]" italics="true" pageId="8" pageNumber="671">N. aski</emphasis>
|
||
</taxonomicName>
|
||
seems to be 8,8,8,7, but the number of claws varies significantly between and within specimens, as illustrated by the
|
||
<typeStatus id="FD5549E83D34FF8429B0FDACFF005B2D" box="[145,243,600,622]" pageId="8" pageNumber="671" type="holotype">holotype</typeStatus>
|
||
, which has a different number of claws on the left (8,9,8,7) and the right sides (8,8,8,8), respectively (
|
||
<tableCitation id="6F6CC2F13D34FF8429BBFD61FF065BE9" box="[154,245,661,683]" captionStart="Table 1" captionStartId="9.[163,227,196,217]" captionTargetPageId="9" captionText="Table 1. Measurements of selected characters on holotypes and paratypes of Neoechiniscoides aski and Neoechiniscoides pollocki" httpUri="http://table.plazi.org/id/7691A7C23D35FF852982FF30FF0759B4" pageId="8" pageNumber="671" tableUuid="7691A7C23D35FF852982FF30FF0759B4">Table 1</tableCitation>
|
||
). The dorsal cuticle is characterized by a polygonal granulation that continues ventrally on the head. The polygons are between 2 and
|
||
<quantity id="E5165AAF3D34FF842B42FD26FD6E5BAB" box="[611,669,722,744]" metricMagnitude="-3" metricUnit="m" metricValue="5.0" pageId="8" pageNumber="671" unit="mm" value="5.0">5 µm</quantity>
|
||
in size, with the size of the granulation decreasing laterally and in depressions of the cuticle. Each polygon has several fibres that connect to neighbouring polygons. The dorsal cuticle is modified in connection with muscle attachment sites (
|
||
<figureCitation id="BAD5EBCF3D34FF84289BFC98FDFC5AC2" box="[442,527,876,898]" captionStart="Figure 3" captionStartId="10.[145,225,1675,1697]" captionTargetBox="[199,1371,195,1635]" captionTargetId="figure-24@10.[199,1371,195,1635]" captionTargetPageId="10" captionText="Figure 3. Scanning electron microscopy of Neoechiniscoides aski. A–C, female. A, habitus of mature female. Scale bar: 10 µm. B, close-up of gonopore with associated cuticular plate and anus. Note muscle attachment sites located lateral to the female gonopore. Scale bar: 10 µm. C, close-up of head region. Note the cuticular sculpture of the head. Scale bar: 10 µm. D–H, male. D, second leg, with dome-shaped sense organ (p2). Scale bar: 1 µm. E, habitus of adult male. Scale bar: 10 µm. F, prominent dorsal cuticular attachment sites of the caudal segment. Scale bar: 1 µm. G, posterioventral part of a male, showing the fourth leg pair, anal system and gonopore. Anterior is to the left. Scale bar: 10 µm. H, close-up of head region of male. Note the relatively large secondary clavae. Scale bar: 1 µm. Abbreviations: an, anus; cE, cirrus E; cl, claw; ec, external" figureDoi="http://doi.org/10.5281/zenodo.4623677" httpUri="https://zenodo.org/record/4623677/files/figure.png" pageId="8" pageNumber="671">Figs 3F</figureCitation>
|
||
,
|
||
<figureCitation id="BAD5EBCF3D34FF842B3DFC98FDD95AC1" box="[540,554,876,898]" captionStart="Figure 5" captionStartId="13.[163,241,1678,1700]" captionTargetBox="[427,1185,205,1632]" captionTargetId="figure-26@13.[420,1185,195,1638]" captionTargetPageId="13" captionText="Figure 5. Neoechiniscoides aski. Drawing of allotypic male (NHMD-230297), dorsal view. Scale bar: 50 µm. Abbreviations: as, anal system; cE, cirrus E; cl, claw; ds, dorsal cuticular attachment sites; ec, external cirrus; fu, furca of the stylet; ic, internal cirrus; lc, lateral cirrus (cirrus A); mg, midgut; p1–p4, sensory structures of legs I–IV, respectively; pc, primary clava; sc, secondary clava; sp, spermatozoa; sv, seminal vesicle; te, testicle." figureDoi="http://doi.org/10.5281/zenodo.4623683" httpUri="https://zenodo.org/record/4623683/files/figure.png" pageId="8" pageNumber="671">5</figureCitation>
|
||
). Specifically, five dorsal cuticular attachment sites (without polygon structure) were observed on the caudal segment. The buccopharyngeal tube is relatively short, and the buccopharyngeal apparatus appears similar to that of
|
||
<taxonomicName id="E5EE8CC93D34FF8429D0FBF1FEBF5D79" authority="sigismundi (M. Schultze, 1865)" authorityName="sigismundi (M. Schultze" authorityYear="1865" baseAuthorityName="M. Schultze" baseAuthorityYear="1865" class="Heterotardigrada" family="Echiniscoididae" genus="Echiniscoides" kingdom="Animalia" order="Echiniscoidea" pageId="8" pageNumber="671" phylum="Tardigrada" rank="subSpecies" species="sigismundi" subSpecies="sigismundi">
|
||
<emphasis id="109A2B583D34FF8429D0FBF1FD365D59" box="[241,709,1029,1050]" italics="true" pageId="8" pageNumber="671">Echiniscoides sigismundi sigismundi</emphasis>
|
||
(M. Schultze, 1865)
|
||
</taxonomicName>
|
||
. The sensory structures are also of
|
||
<emphasis id="109A2B583D34FF8429B0FBB7FEBE5D14" box="[145,333,1090,1112]" italics="true" pageId="8" pageNumber="671">
|
||
E. s.
|
||
<taxonomicName id="E5EE8CC93D34FF8429EBFBB6FEBE5D14" baseAuthorityName="M. Schultze" baseAuthorityYear="1865" box="[202,333,1090,1111]" class="Heterotardigrada" family="Echiniscoididae" genus="Echiniscoides" kingdom="Animalia" order="Echiniscoidea" pageId="8" pageNumber="671" phylum="Tardigrada" rank="species" species="sigismundi">sigismundi</taxonomicName>
|
||
</emphasis>
|
||
<typeStatus id="FD5549E83D34FF842877FBB7FE785D1B" box="[342,395,1091,1112]" pageId="8" pageNumber="671">type</typeStatus>
|
||
: black to dark brown eyes, two small domes (p1 and p2) on leg Iand II, respectively, and p3 is a seta. The sensory structure on leg IV is similar to the primary clava; both are a papillary structure with a tiny spine. Cirri Aand Eare short, with a few spiral striations at the base. The secondary clava appear different in males compared with females. In females, they seem flatter and are surrounded by a ring of unsculptured cuticle (
|
||
<figureCitation id="BAD5EBCF3D34FF842BBDFACCFD005C0E" box="[668,755,1336,1358]" captionStart="Figure 3" captionStartId="10.[145,225,1675,1697]" captionTargetBox="[199,1371,195,1635]" captionTargetId="figure-24@10.[199,1371,195,1635]" captionTargetPageId="10" captionText="Figure 3. Scanning electron microscopy of Neoechiniscoides aski. A–C, female. A, habitus of mature female. Scale bar: 10 µm. B, close-up of gonopore with associated cuticular plate and anus. Note muscle attachment sites located lateral to the female gonopore. Scale bar: 10 µm. C, close-up of head region. Note the cuticular sculpture of the head. Scale bar: 10 µm. D–H, male. D, second leg, with dome-shaped sense organ (p2). Scale bar: 1 µm. E, habitus of adult male. Scale bar: 10 µm. F, prominent dorsal cuticular attachment sites of the caudal segment. Scale bar: 1 µm. G, posterioventral part of a male, showing the fourth leg pair, anal system and gonopore. Anterior is to the left. Scale bar: 10 µm. H, close-up of head region of male. Note the relatively large secondary clavae. Scale bar: 1 µm. Abbreviations: an, anus; cE, cirrus E; cl, claw; ec, external" figureDoi="http://doi.org/10.5281/zenodo.4623677" httpUri="https://zenodo.org/record/4623677/files/figure.png" pageId="8" pageNumber="671">Figs 3C</figureCitation>
|
||
,
|
||
<figureCitation id="BAD5EBCF3D34FF8429B0FAA2FF6C5C28" box="[145,159,1366,1387]" captionStart="Figure 4" captionStartId="12.[145,223,1678,1700]" captionTargetBox="[380,1189,196,1637]" captionTargetId="figure-25@12.[379,1191,195,1638]" captionTargetPageId="12" captionText="Figure 4. Neoechiniscoides aski.Drawing of holotypicfemale (NHMD-230293),ventral view.Scale bar:50 µm.Abbreviations: an, anus; bt, buccal tube; cE, cirrus E; cm, claw membrane; ec, external cirrus; ey, eye; go, gonopore; ic, internal cirrus; lc, lateral cirrus (cirrus A); ma, lateral attachment sites associated with the female gonopore; mg, midgut; nc, new claws forming inside claw gland; oo, oocyte; ov, ovary; p1–p3, sensory structures of legs I–III, respectively; pa, ventral cuticular plate; pb, pharyngeal bulb; pc, primary clava; pl, placoid; sc, secondary clava; wl, lateral wing of the anal structure." figureDoi="http://doi.org/10.5281/zenodo.4623679" httpUri="https://zenodo.org/record/4623679/files/figure.png" pageId="8" pageNumber="671">4</figureCitation>
|
||
), whereas the male has a slightly more protruding secondary clava that can be seen from the dorsal side (
|
||
<figureCitation id="BAD5EBCF3D34FF8429F0FA60FEDD5CEA" box="[209,302,1428,1450]" captionStart="Figure 3" captionStartId="10.[145,225,1675,1697]" captionTargetBox="[199,1371,195,1635]" captionTargetId="figure-24@10.[199,1371,195,1635]" captionTargetPageId="10" captionText="Figure 3. Scanning electron microscopy of Neoechiniscoides aski. A–C, female. A, habitus of mature female. Scale bar: 10 µm. B, close-up of gonopore with associated cuticular plate and anus. Note muscle attachment sites located lateral to the female gonopore. Scale bar: 10 µm. C, close-up of head region. Note the cuticular sculpture of the head. Scale bar: 10 µm. D–H, male. D, second leg, with dome-shaped sense organ (p2). Scale bar: 1 µm. E, habitus of adult male. Scale bar: 10 µm. F, prominent dorsal cuticular attachment sites of the caudal segment. Scale bar: 1 µm. G, posterioventral part of a male, showing the fourth leg pair, anal system and gonopore. Anterior is to the left. Scale bar: 10 µm. H, close-up of head region of male. Note the relatively large secondary clavae. Scale bar: 1 µm. Abbreviations: an, anus; cE, cirrus E; cl, claw; ec, external" figureDoi="http://doi.org/10.5281/zenodo.4623677" httpUri="https://zenodo.org/record/4623677/files/figure.png" pageId="8" pageNumber="671">Figs 3H</figureCitation>
|
||
,
|
||
<figureCitation id="BAD5EBCF3D34FF84281CFA67FEB85CEA" box="[317,331,1427,1449]" captionStart="Figure 5" captionStartId="13.[163,241,1678,1700]" captionTargetBox="[427,1185,205,1632]" captionTargetId="figure-26@13.[420,1185,195,1638]" captionTargetPageId="13" captionText="Figure 5. Neoechiniscoides aski. Drawing of allotypic male (NHMD-230297), dorsal view. Scale bar: 50 µm. Abbreviations: as, anal system; cE, cirrus E; cl, claw; ds, dorsal cuticular attachment sites; ec, external cirrus; fu, furca of the stylet; ic, internal cirrus; lc, lateral cirrus (cirrus A); mg, midgut; p1–p4, sensory structures of legs I–IV, respectively; pc, primary clava; sc, secondary clava; sp, spermatozoa; sv, seminal vesicle; te, testicle." figureDoi="http://doi.org/10.5281/zenodo.4623683" httpUri="https://zenodo.org/record/4623683/files/figure.png" pageId="8" pageNumber="671">5</figureCitation>
|
||
). In both sexes, the secondary clava attach directly on the head (not as a projection as in
|
||
<emphasis id="109A2B583D34FF8429B0FA26FEB75CA5" box="[145,324,1489,1511]" italics="true" pageId="8" pageNumber="671">
|
||
E. s.
|
||
<taxonomicName id="E5EE8CC93D34FF8429E4FA25FEB75CA5" baseAuthorityName="M. Schultze" baseAuthorityYear="1865" box="[197,324,1489,1510]" class="Heterotardigrada" family="Echiniscoididae" genus="Echiniscoides" kingdom="Animalia" order="Echiniscoidea" pageId="8" pageNumber="671" phylum="Tardigrada" rank="species" species="sigismundi">sigismundi</taxonomicName>
|
||
</emphasis>
|
||
). The gonopore of the female is unique within the
|
||
<taxonomicName id="E5EE8CC93D34FF842833FA04FE3A5F45" authorityName="Kristensen & Hallas" authorityYear="1980" box="[274,457,1520,1542]" class="Heterotardigrada" family="Echiniscoididae" kingdom="Animalia" order="Echiniscoidea" pageId="8" pageNumber="671" phylum="Tardigrada" rank="family">Echiniscoididae</taxonomicName>
|
||
and is formed of six large units positioned on a ring-shaped cuticular protrusion. Two small depressions (
|
||
<figureCitation id="BAD5EBCF3D34FF8428BCF9D9FE015F01" box="[413,498,1581,1603]" captionStart="Figure 3" captionStartId="10.[145,225,1675,1697]" captionTargetBox="[199,1371,195,1635]" captionTargetId="figure-24@10.[199,1371,195,1635]" captionTargetPageId="10" captionText="Figure 3. Scanning electron microscopy of Neoechiniscoides aski. A–C, female. A, habitus of mature female. Scale bar: 10 µm. B, close-up of gonopore with associated cuticular plate and anus. Note muscle attachment sites located lateral to the female gonopore. Scale bar: 10 µm. C, close-up of head region. Note the cuticular sculpture of the head. Scale bar: 10 µm. D–H, male. D, second leg, with dome-shaped sense organ (p2). Scale bar: 1 µm. E, habitus of adult male. Scale bar: 10 µm. F, prominent dorsal cuticular attachment sites of the caudal segment. Scale bar: 1 µm. G, posterioventral part of a male, showing the fourth leg pair, anal system and gonopore. Anterior is to the left. Scale bar: 10 µm. H, close-up of head region of male. Note the relatively large secondary clavae. Scale bar: 1 µm. Abbreviations: an, anus; cE, cirrus E; cl, claw; ec, external" figureDoi="http://doi.org/10.5281/zenodo.4623677" httpUri="https://zenodo.org/record/4623677/files/figure.png" pageId="8" pageNumber="671">Figs 3B</figureCitation>
|
||
,
|
||
<figureCitation id="BAD5EBCF3D34FF8428DFF9D9FDFF5F01" box="[510,524,1581,1602]" captionStart="Figure 4" captionStartId="12.[145,223,1678,1700]" captionTargetBox="[380,1189,196,1637]" captionTargetId="figure-25@12.[379,1191,195,1638]" captionTargetPageId="12" captionText="Figure 4. Neoechiniscoides aski.Drawing of holotypicfemale (NHMD-230293),ventral view.Scale bar:50 µm.Abbreviations: an, anus; bt, buccal tube; cE, cirrus E; cm, claw membrane; ec, external cirrus; ey, eye; go, gonopore; ic, internal cirrus; lc, lateral cirrus (cirrus A); ma, lateral attachment sites associated with the female gonopore; mg, midgut; nc, new claws forming inside claw gland; oo, oocyte; ov, ovary; p1–p3, sensory structures of legs I–III, respectively; pa, ventral cuticular plate; pb, pharyngeal bulb; pc, primary clava; pl, placoid; sc, secondary clava; wl, lateral wing of the anal structure." figureDoi="http://doi.org/10.5281/zenodo.4623679" httpUri="https://zenodo.org/record/4623679/files/figure.png" pageId="8" pageNumber="671">4</figureCitation>
|
||
) are situated close to the gonopore; we interpret these as muscle attachment sites. Asmall cuticular plate is present in front of the gonopore (
|
||
<figureCitation id="BAD5EBCF3D34FF84282AF97DFE965FDD" box="[267,357,1673,1695]" captionStart="Figure 3" captionStartId="10.[145,225,1675,1697]" captionTargetBox="[199,1371,195,1635]" captionTargetId="figure-24@10.[199,1371,195,1635]" captionTargetPageId="10" captionText="Figure 3. Scanning electron microscopy of Neoechiniscoides aski. A–C, female. A, habitus of mature female. Scale bar: 10 µm. B, close-up of gonopore with associated cuticular plate and anus. Note muscle attachment sites located lateral to the female gonopore. Scale bar: 10 µm. C, close-up of head region. Note the cuticular sculpture of the head. Scale bar: 10 µm. D–H, male. D, second leg, with dome-shaped sense organ (p2). Scale bar: 1 µm. E, habitus of adult male. Scale bar: 10 µm. F, prominent dorsal cuticular attachment sites of the caudal segment. Scale bar: 1 µm. G, posterioventral part of a male, showing the fourth leg pair, anal system and gonopore. Anterior is to the left. Scale bar: 10 µm. H, close-up of head region of male. Note the relatively large secondary clavae. Scale bar: 1 µm. Abbreviations: an, anus; cE, cirrus E; cl, claw; ec, external" figureDoi="http://doi.org/10.5281/zenodo.4623677" httpUri="https://zenodo.org/record/4623677/files/figure.png" pageId="8" pageNumber="671">Figs 3B</figureCitation>
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,
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<figureCitation id="BAD5EBCF3D34FF842852F97DFE725FDD" box="[371,385,1673,1694]" captionStart="Figure 4" captionStartId="12.[145,223,1678,1700]" captionTargetBox="[380,1189,196,1637]" captionTargetId="figure-25@12.[379,1191,195,1638]" captionTargetPageId="12" captionText="Figure 4. Neoechiniscoides aski.Drawing of holotypicfemale (NHMD-230293),ventral view.Scale bar:50 µm.Abbreviations: an, anus; bt, buccal tube; cE, cirrus E; cm, claw membrane; ec, external cirrus; ey, eye; go, gonopore; ic, internal cirrus; lc, lateral cirrus (cirrus A); ma, lateral attachment sites associated with the female gonopore; mg, midgut; nc, new claws forming inside claw gland; oo, oocyte; ov, ovary; p1–p3, sensory structures of legs I–III, respectively; pa, ventral cuticular plate; pb, pharyngeal bulb; pc, primary clava; pl, placoid; sc, secondary clava; wl, lateral wing of the anal structure." figureDoi="http://doi.org/10.5281/zenodo.4623679" httpUri="https://zenodo.org/record/4623679/files/figure.png" pageId="8" pageNumber="671">4</figureCitation>
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); males lack this structure. The anal system consists of three lobes: two lateral and a terminal lobe. The anus appears as an opening at the junction between the three lobes. The lateral lobes of the anal systems appear as a double structure, with two wings (
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<figureCitation id="BAD5EBCF3D34FF842A98FEB4FBC45816" box="[953,1079,320,342]" captionStart="Figure 2" captionStartId="7.[163,241,1675,1697]" captionTargetBox="[265,1371,158,1636]" captionTargetId="figure-26@7.[631,1340,510,914]" captionTargetPageId="7" captionText="Figure 2. Light microscopy of Neoechiniscoides aski. A–C, images of live, moving specimens. A, ventral view of a live adult female, showing the anal system with lateral wings. In live animals, the conspicuous anal complex moves from side to side when the animal walks/runs. Scale bar: 50 µm. B, dorsal view of live adult female used for DNA extraction (28S GenBank accession number KX363645; COI GenBank accession number KX363656). Note the eight to nine mature oocytes. Scale bar: 50 µm. C, dorsal view of live adult male. Scale bar: 50 µm. D, holotype of N. aski (female). Scale bar: 50 µm. E, paratypic allotype of N. aski (male). Scale bar: 50 µm. F, ventrocaudal view of holotypic female showing anal system and the gonopore, which is raised on an elevation (note that seminal receptacles are lacking). Scale bar: 20 µm. G, ventral view of paratypic" figureDoi="http://doi.org/10.5281/zenodo.4623673" httpUri="https://zenodo.org/record/4623673/files/figure.png" pageId="8" pageNumber="671">Figs 2A, F</figureCitation>
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,
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<figureCitation id="BAD5EBCF3D34FF842D69FEB4FB7F5816" box="[1096,1164,320,341]" captionStart="Figure 3" captionStartId="10.[145,225,1675,1697]" captionTargetBox="[199,1371,195,1635]" captionTargetId="figure-24@10.[199,1371,195,1635]" captionTargetPageId="10" captionText="Figure 3. Scanning electron microscopy of Neoechiniscoides aski. A–C, female. A, habitus of mature female. Scale bar: 10 µm. B, close-up of gonopore with associated cuticular plate and anus. Note muscle attachment sites located lateral to the female gonopore. Scale bar: 10 µm. C, close-up of head region. Note the cuticular sculpture of the head. Scale bar: 10 µm. D–H, male. D, second leg, with dome-shaped sense organ (p2). Scale bar: 1 µm. E, habitus of adult male. Scale bar: 10 µm. F, prominent dorsal cuticular attachment sites of the caudal segment. Scale bar: 1 µm. G, posterioventral part of a male, showing the fourth leg pair, anal system and gonopore. Anterior is to the left. Scale bar: 10 µm. H, close-up of head region of male. Note the relatively large secondary clavae. Scale bar: 1 µm. Abbreviations: an, anus; cE, cirrus E; cl, claw; ec, external" figureDoi="http://doi.org/10.5281/zenodo.4623677" httpUri="https://zenodo.org/record/4623677/files/figure.png" pageId="8" pageNumber="671">3B, G</figureCitation>
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<figureCitation id="BAD5EBCF3D34FF842DBDFEB4FB595816" box="[1180,1194,320,341]" captionStart="Figure 4" captionStartId="12.[145,223,1678,1700]" captionTargetBox="[380,1189,196,1637]" captionTargetId="figure-25@12.[379,1191,195,1638]" captionTargetPageId="12" captionText="Figure 4. Neoechiniscoides aski.Drawing of holotypicfemale (NHMD-230293),ventral view.Scale bar:50 µm.Abbreviations: an, anus; bt, buccal tube; cE, cirrus E; cm, claw membrane; ec, external cirrus; ey, eye; go, gonopore; ic, internal cirrus; lc, lateral cirrus (cirrus A); ma, lateral attachment sites associated with the female gonopore; mg, midgut; nc, new claws forming inside claw gland; oo, oocyte; ov, ovary; p1–p3, sensory structures of legs I–III, respectively; pa, ventral cuticular plate; pb, pharyngeal bulb; pc, primary clava; pl, placoid; sc, secondary clava; wl, lateral wing of the anal structure." figureDoi="http://doi.org/10.5281/zenodo.4623679" httpUri="https://zenodo.org/record/4623679/files/figure.png" pageId="8" pageNumber="671">4</figureCitation>
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,
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<figureCitation id="BAD5EBCF3D34FF842D9DFECBFB395816" box="[1212,1226,319,341]" captionStart="Figure 5" captionStartId="13.[163,241,1678,1700]" captionTargetBox="[427,1185,205,1632]" captionTargetId="figure-26@13.[420,1185,195,1638]" captionTargetPageId="13" captionText="Figure 5. Neoechiniscoides aski. Drawing of allotypic male (NHMD-230297), dorsal view. Scale bar: 50 µm. Abbreviations: as, anal system; cE, cirrus E; cl, claw; ds, dorsal cuticular attachment sites; ec, external cirrus; fu, furca of the stylet; ic, internal cirrus; lc, lateral cirrus (cirrus A); mg, midgut; p1–p4, sensory structures of legs I–IV, respectively; pc, primary clava; sc, secondary clava; sp, spermatozoa; sv, seminal vesicle; te, testicle." figureDoi="http://doi.org/10.5281/zenodo.4623683" httpUri="https://zenodo.org/record/4623683/files/figure.png" pageId="8" pageNumber="671">5</figureCitation>
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<figureCitation id="BAD5EBCF3D34FF842DFAFECBFB1A5816" box="[1243,1257,319,341]" captionStart="Figure 6" captionStartId="14.[146,225,1001,1023]" captionTargetBox="[370,1201,196,960]" captionTargetId="figure-362@14.[369,1201,195,961]" captionTargetPageId="14" captionText="Figure 6. Drawing of anal system in Isoechiniscoides, Echiniscoides and Neoechiniscoides based on investigations of live animals and preparations on slides for light microscopy. In all three genera, a three-lobed anal system is present and consists of two lateral lobes and a terminal lobe of variable size. The anal opening is indicated in black. In Neoechiniscoides, two wings are present on the lateral lobes. These wings are easily visualized in live animals and using light microscopic preparations, whereas this double structure of the lateral lobes is difficult to visualize in specimens prepared for scanning electron microscopy. Scale bar: 10 µm." figureDoi="http://doi.org/10.5281/zenodo.4623687" httpUri="https://zenodo.org/record/4623687/files/figure.png" pageId="8" pageNumber="671">6</figureCitation>
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). We have no transmission electron microscopy of these lobes, but hypothesize that they might be glandular.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |