271 lines
19 KiB
XML
271 lines
19 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.77.59852" ID-GBIF-Dataset="78bab002-8c3e-4548-8377-cb082fa11d13" ID-PMC="PMC7819953" ID-Pensoft-Pub="1314-4049-77-65" ID-Pensoft-UUID="5D8D5A6329A4584ABC11C425E4B0E47A" ID-PubMed="33519269" ModsDocID="1314-4049-77-65" checkinTime="1610662561303" checkinUser="pensoft" docAuthor="Sun, Wenxiu, Huang, Shengting, Xia, Jiwen, Zhang, Xiuguo & Li, Zhuang" docDate="2021" docId="4498E3D793FF51B48A51F81894354C95" docLanguage="en" docName="MycoKeys 77: 65-95" docOrigin="MycoKeys 77" docPubDate="2021-01-14" docSource="http://dx.doi.org/10.3897/mycokeys.77.59852" docTitle="Diaporthe lutescens S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li 2021, sp. nov." docType="treatment" docVersion="4" id="5D8D5A6329A4584ABC11C425E4B0E47A" lastPageNumber="65" masterDocId="5D8D5A6329A4584ABC11C425E4B0E47A" masterDocTitle="Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species" masterLastPageNumber="95" masterPageNumber="65" pageNumber="65" updateTime="1668136608950" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Sun, Wenxiu</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Huang, Shengting</mods:namePart>
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<mods:affiliation>College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xia, Jiwen</mods:namePart>
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<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7436-7249</mods:nameIdentifier>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Xiuguo</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Li, Zhuang</mods:namePart>
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<mods:affiliation>Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China</mods:affiliation>
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<mods:nameIdentifier type="email">junwuxue@126.com</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2021</mods:date>
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<mods:detail type="pubDate">
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<mods:number>2021-01-14</mods:number>
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</mods:detail>
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<mods:detail type="volume">
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<mods:number>77</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>65</mods:start>
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<mods:end>95</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.77.59852</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.77.59852</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-77-65</mods:identifier>
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<mods:identifier type="Pensoft-UUID">5D8D5A6329A4584ABC11C425E4B0E47A</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="176584079" LSID="urn:lsid:plazi:treatment:4498E3D793FF51B48A51F81894354C95" httpUri="http://treatment.plazi.org/id/4498E3D793FF51B48A51F81894354C95" lastPageNumber="65" pageId="0" pageNumber="65">
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<subSubSection pageId="0" pageNumber="65" type="nomenclature">
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName LSID="4498E3D7-93FF-51B4-8A51-F81894354C95" authority="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe lutescens" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="lutescens" status="sp. nov.">Diaporthe lutescens S.T. Huang, J.W. Xia, X.G. Zhang & Z. Li</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="65">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 7" captionStartId="F8" captionText="Figure 7. Diaporthe lutescens (SAUCC 194.36) a leaves of host plant b, c surface (b) and reverse (c) sides of colony after incubation for 15 days on PDA d conidiomata e-g conidiophores and conidiogenous cells h, i beta conidia. Scale bars: 10 μm (e-i)." figureDoi="10.3897/mycokeys.77.59852.figure7" httpUri="https://binary.pensoft.net/fig/497510" pageId="0" pageNumber="65">Figure 7</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="etymology">
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<paragraph pageId="0" pageNumber="65">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Named after the host
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<taxonomicName class="Liliopsida" family="Arecaceae" genus="Chrysalidocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Chrysalidocarpus lutescens" order="Arecales" pageId="0" pageNumber="65" phylum="Magnoliophyta" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="65">Chrysalidocarpus lutescens</emphasis>
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</taxonomicName>
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on which it was collected.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="diagnosis">
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<paragraph pageId="0" pageNumber="65">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe lutescens" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe lutescens</emphasis>
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</taxonomicName>
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differs from
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<taxonomicName lsidName="D. pterocarpi" pageId="0" pageNumber="65" rank="species" species="pterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pterocarpi</emphasis>
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</taxonomicName>
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(S. Hughes) D. Udayanga et al. and
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<taxonomicName lsidName="D. pseudoinconspicua" pageId="0" pageNumber="65" rank="species" species="pseudoinconspicua">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pseudoinconspicua</emphasis>
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</taxonomicName>
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T.G.L. Oliveira et al. in longer beta conidia and the types of conidia.
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</paragraph>
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<caption doi="10.3897/mycokeys.77.59852.figure7" httpUri="https://binary.pensoft.net/fig/497510" pageId="0" pageNumber="65" start="Figure 7" startId="F8">
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<paragraph pageId="0" pageNumber="65">
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<emphasis bold="true" pageId="0" pageNumber="65">Figure 7.</emphasis>
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe lutescens" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe lutescens</emphasis>
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</taxonomicName>
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(SAUCC194.36)
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<emphasis bold="true" pageId="0" pageNumber="65">a</emphasis>
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leaves of host plant
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<emphasis bold="true" pageId="0" pageNumber="65">b, c</emphasis>
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surface (
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<emphasis bold="true" pageId="0" pageNumber="65">b</emphasis>
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) and reverse (
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<emphasis bold="true" pageId="0" pageNumber="65">c</emphasis>
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) sides of colony after incubation for 15 days on PDA
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<emphasis bold="true" pageId="0" pageNumber="65">d</emphasis>
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conidiomata
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<emphasis bold="true" pageId="0" pageNumber="65">e-g</emphasis>
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conidiophores and conidiogenous cells
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<emphasis bold="true" pageId="0" pageNumber="65">h, i</emphasis>
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beta conidia. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="65">e-i</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="materials_examined">
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<paragraph pageId="0" pageNumber="65">Type.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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China, Yunnan Province: Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, on leaves of
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<taxonomicName class="Liliopsida" family="Arecaceae" genus="Chrysalidocarpus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Chrysalidocarpus lutescens" order="Arecales" pageId="0" pageNumber="65" phylum="Magnoliophyta" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="65">Chrysalidocarpus lutescens</emphasis>
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</taxonomicName>
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. 19 April 2019, S.T. Huang, HSAUP194.36, holotype, ex-holotype living culture SAUCC194.36.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Description.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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Asexual morph: Conidiomata pycnidial, scattered or aggregated, black, erumpent, slightly raised above the surface of the culture medium, subglobose, exuding white creamy conidial droplets from central ostioles after 30 days incubation in light condition at 25 °C on PDA; pycnidial wall consists of black to dark brown, thin-walled cells. Conidiophores 10.2-17.0
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<normalizedToken originalValue="×">x</normalizedToken>
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1.8-3.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, hyaline, unbranched, subcylindrical, septate, smooth, straight or slightly curved, obtuse at the apex, widened at base. Conidiogenous cells 5.7-9.1
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<normalizedToken originalValue="×">x</normalizedToken>
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1.4-2.6
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, phialidic, cylindrical, terminal, straight to sinuous, tapering towards the apex. Beta conidia 20.8-28.8
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<normalizedToken originalValue="×">x</normalizedToken>
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1.2-2.0
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<normalizedToken originalValue="μm">μm</normalizedToken>
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(mean = 25.3
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<normalizedToken originalValue="×">x</normalizedToken>
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1.4
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, n = 20), filiform, hyaline, straight or slightly curved, aseptate, base subtruncate, enlarged towards the apex. Alpha conidia and gamma conidia not observed.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="description">
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<paragraph pageId="0" pageNumber="65">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="65">Pure culture was isolated by subbing hyphal tips growing from surface sterilized infected plant material. Colonies on PDA cover the petri plate diameter after incubation for 15 days in dark conditions at 25 °C, initially white, becoming grayish, reverse pale brown, with concentric rings of dense and sparse hyphae, irregular margin, fluffy aerial mycelium. Pycnidia formed in 15 days.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="65" type="notes">
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<paragraph pageId="0" pageNumber="65">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="65">
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From the phylotree, seen on Fig.
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Phylogram of Diaporthe based on combined ITS, TUB, TEF, CAL and HIS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Strains from this study are shown in red. Three branches were shortened to fit the page size - these are indicated by symbol (//) with indication number showing how many times they are shortened." figureDoi="10.3897/mycokeys.77.59852.figure1" httpUri="https://binary.pensoft.net/fig/497579" pageId="0" pageNumber="65">1</figureCitation>
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,
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe lutescens" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe lutescens</emphasis>
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</taxonomicName>
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forms an independent clade and is phylogenetically distinct from
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<taxonomicName lsidName="D. pterocarpi" pageId="0" pageNumber="65" rank="species" species="pterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pterocarpi</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="D. pseudoinconspicua" pageId="0" pageNumber="65" rank="species" species="pseudoinconspicua">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pseudoinconspicua</emphasis>
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</taxonomicName>
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.
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<taxonomicName authorityName="S. T. Huang, J. W. Xia, X. G. Zhang & Z. Li" authorityYear="2021" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe lutescens" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe lutescens</emphasis>
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</taxonomicName>
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can be distinguished from
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<taxonomicName lsidName="D. pterocarpi" pageId="0" pageNumber="65" rank="species" species="pterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pterocarpi</emphasis>
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</taxonomicName>
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in ITS,
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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,
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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and
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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loci by 77 nucleotide differences in concatenated alignment (43 in ITS, 2 in
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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, 29 in
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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and 17 in
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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), and from
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<taxonomicName lsidName="D. pseudoinconspicua" pageId="0" pageNumber="65" rank="species" species="pseudoinconspicua">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pseudoinconspicua</emphasis>
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</taxonomicName>
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in ITS,
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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,
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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,
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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and
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<emphasis italics="true" pageId="0" pageNumber="65">HIS</emphasis>
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loci by 65 nucleotide differences (18 in ITS, 3 in
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<emphasis italics="true" pageId="0" pageNumber="65">TUB</emphasis>
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, 23 in
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<emphasis italics="true" pageId="0" pageNumber="65">TEF</emphasis>
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, 8 in
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<emphasis italics="true" pageId="0" pageNumber="65">CAL</emphasis>
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and 13 in
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<emphasis italics="true" pageId="0" pageNumber="65">HIS</emphasis>
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). Moreover,
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<taxonomicName lsidName="D. lutescens" pageId="0" pageNumber="65" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="65">D. lutescens</emphasis>
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</taxonomicName>
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differs from
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<taxonomicName lsidName="D. pterocarpi" pageId="0" pageNumber="65" rank="species" species="pterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pterocarpi</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="D. pseudoinconspicua" pageId="0" pageNumber="65" rank="species" species="pseudoinconspicua">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pseudoinconspicua</emphasis>
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</taxonomicName>
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in having longer beta conidia (20.8-28.8
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<normalizedToken originalValue="×">x</normalizedToken>
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1.2-2.0 vs. 16.0-23.4
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<normalizedToken originalValue="×">x</normalizedToken>
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||
1.0-1.4
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<normalizedToken originalValue="μm">μm</normalizedToken>
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, and 20.8-28.8
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<normalizedToken originalValue="×">x</normalizedToken>
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1.2-2.0 vs. 18.0-21.0
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<normalizedToken originalValue="×">x</normalizedToken>
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1.0-1.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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). Furthermore,
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<taxonomicName authorityName="Udayanga, Xing Z. Liu & K.D. Hyde" authorityYear="2012" baseAuthorityName="S. Hughes" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe pterocarpi" order="Diaporthales" pageId="0" pageNumber="65" phylum="Ascomycota" rank="species" species="pterocarpi">
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<emphasis italics="true" pageId="0" pageNumber="65">Diaporthe pterocarpi</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="D. pseudoinconspicua" pageId="0" pageNumber="65" rank="species" species="pseudoinconspicua">
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<emphasis italics="true" pageId="0" pageNumber="65">D. pseudoinconspicua</emphasis>
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</taxonomicName>
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can produce
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<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
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, but
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<taxonomicName lsidName="D. lutescens" pageId="0" pageNumber="65" rank="species" species="lutescens">
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<emphasis italics="true" pageId="0" pageNumber="65">D. lutescens</emphasis>
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</taxonomicName>
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cannot (
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<bibRefCitation author="Crous, PW" journalOrPublisher="Persoonia" pageId="0" pageNumber="65" pagination="240 - 393" refId="B10" refString="Crous, PW, Wingfield, MJ, Burgess, TI, Hardy, GEStJ, Gene, J, Guarro, J, Baseia, IG, Garcia, D, Gusmao, LFP, Souza-Motta, CM, 2018a. Fungal planet description sheets: 716-784. Persoonia 40 (1): 240 - 393" title="Fungal planet description sheets: 716 - 784." volume="40" year="2018 a">Crous et al. 2018a</bibRefCitation>
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;
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<bibRefCitation DOI="https://doi.org/10.1094/PDIS-08-19-1655-PDN" author="Broge, M" journalOrPublisher="Plant Disease" pageId="0" pageNumber="65" pagination="1550 - 1550" refId="B2" refString="Broge, M, Howard, A, Biles, CL, Udayanga, D, Taff, H, Dudley, L, Bruton, BD, 2020. First report of Diaporthe fruit rot of melons caused by D. Pterocarpi in Costa Rica. Plant Disease 104 (5): 1550 - 1550, DOI: https://doi.org/10.1094/PDIS-08-19-1655-PDN" title="First report of Diaporthe fruit rot of melons caused by D. Pterocarpi in Costa Rica." url="https://doi.org/10.1094/PDIS-08-19-1655-PDN" volume="104" year="2020">Broge et al. 2020</bibRefCitation>
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).
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |