289 lines
16 KiB
XML
289 lines
16 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.57.35448" ID-GBIF-Dataset="bf107fa5-ec57-420f-bb6d-8c2853845429" ID-PMC="PMC6717119" ID-Pensoft-Pub="1314-4049-57-113" ID-Pensoft-UUID="5EEB9835D93A5DD7B2C25ABC5216FD7B" ID-PubMed="31523165" ModsDocID="1314-4049-57-113" checkinTime="1566623487220" checkinUser="pensoft" docAuthor="Long, Hui, Zhang, Qian, Hao, Yuan-Yuan, Shao, Xian-Qiang, Wei, Xiao-Xing, Hyde, Kevin D., Wang, Yong & Zhao, De-Gang" docDate="2019" docId="18E3940B229E5A739D4216010B5CBA6D" docLanguage="en" docName="MycoKeys 57: 113-127" docOrigin="MycoKeys 57" docSource="http://dx.doi.org/10.3897/mycokeys.57.35448" docTitle="Diaporthe millettiae H. Long, K. D. Hyde & Yong Wang bis, sp. nov." docType="treatment" docVersion="4" id="5EEB9835D93A5DD7B2C25ABC5216FD7B" lastPageNumber="113" masterDocId="5EEB9835D93A5DD7B2C25ABC5216FD7B" masterDocTitle="Diaporthe species in south-western China" masterLastPageNumber="127" masterPageNumber="113" pageNumber="113" updateTime="1668136291731" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Diaporthe species in south-western China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Long, Hui</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhang, Qian</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hao, Yuan-Yuan</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Shao, Xian-Qiang</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Wei, Xiao-Xing</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hyde, Kevin D.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Wang, Yong</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Zhao, De-Gang</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2019</mods:date>
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<mods:detail type="volume">
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<mods:number>57</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>113</mods:start>
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<mods:end>127</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.57.35448</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.57.35448</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-57-113</mods:identifier>
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</mods:mods>
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<treatment ID-GBIF-Taxon="159244658" LSID="urn:lsid:plazi:treatment:18E3940B229E5A739D4216010B5CBA6D" httpUri="http://treatment.plazi.org/id/18E3940B229E5A739D4216010B5CBA6D" lastPageNumber="113" pageId="0" pageNumber="113">
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<subSubSection pageId="0" pageNumber="113" type="nomenclature">
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<paragraph pageId="0" pageNumber="113">
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<taxonomicName LSID="18e3940b-229e-5a73-9d42-16010b5cba6d" authority="H. Long, K. D. Hyde & Yong Wang bis" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe millettiae" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="millettiae">Diaporthe millettiae H. Long, K.D. Hyde & Yong Wang bis</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="113">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Diaporthe millettiae (GUCC 9167). a-b upper (a) and lower (b) surface of colony on PDA c-d conidiomata e-f conidiophores, conidiogenous loci and conidia g β-conidia h α-conidia. Scale bars: 20 µm (e, f), 10 µm (g, h)." figureDoi="10.3897/mycokeys.57.35448.figure3" httpUri="https://binary.pensoft.net/fig/328113" pageId="0" pageNumber="113">Figure 3</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="113" type="diagnosis">
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<paragraph pageId="0" pageNumber="113">Diagnosis.</paragraph>
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<paragraph pageId="0" pageNumber="113">
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Characterised by larger J-shaped
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<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
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.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="113" type="materials_examined">
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<paragraph pageId="0" pageNumber="113">Type.</paragraph>
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<paragraph pageId="0" pageNumber="113">
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China, Guangxi Province, Nanning City, from leaves of
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<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Millettia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Millettia reticulata" order="Fabales" pageId="0" pageNumber="113" phylum="Tracheophyta" rank="species" species="reticulata">
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<emphasis italics="true" pageId="0" pageNumber="113">Millettia reticulata</emphasis>
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</taxonomicName>
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, 20 September 2016, Y. Wang, HGUP 9167, holotype, ex-type living culture GUCC 9167.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="113" type="description">
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<paragraph pageId="0" pageNumber="113">Description.</paragraph>
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<paragraph pageId="0" pageNumber="113">
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<emphasis italics="true" pageId="0" pageNumber="113">Colonies</emphasis>
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on PDA attaining 9 cm diam. after 10 days; coralloid with feathery branches at margin, adpressed, with apparent aerial mycelium, with numerous irregularly zonate dark stromata, isabelline becoming lighter towards the margin; reverse similar to surface, with zonations.
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<emphasis italics="true" pageId="0" pageNumber="113">Conidiomata</emphasis>
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pycnidial, multilocular, scattered, abundant on PDA after 3 wks, subglobose to irregular, 1.5-1.8 mm diam., ostiolate, with up to 1 mm necks when present.
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<emphasis italics="true" pageId="0" pageNumber="113">Conidiophores</emphasis>
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formed from the inner layer of the locular wall, sometimes reduced to conidiogenous cells, when present 1-septate, hyaline to pale yellowish-brown, cylindrical, 10-23
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<normalizedToken originalValue="×">x</normalizedToken>
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1-2.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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<emphasis italics="true" pageId="0" pageNumber="113">Conidiogenous cells</emphasis>
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cylindrical to flexuous, tapered towards apex, hyaline, 8-18
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-3
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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<emphasis italics="true" pageId="0" pageNumber="113">Alpha conidia</emphasis>
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abundant, fusiform, narrowed towards apex and base, mostly biguttulate, hyaline, 4.5-9
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<normalizedToken originalValue="×">x</normalizedToken>
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2-3.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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<emphasis italics="true" pageId="0" pageNumber="113">Beta conidia</emphasis>
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scarce to abundant, flexuous to J-shaped, hyaline, 17.5-32
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<normalizedToken originalValue="×">x</normalizedToken>
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1-2
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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<emphasis italics="true" pageId="0" pageNumber="113">Perithecia</emphasis>
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not seen.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="113" type="habitat">
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<paragraph pageId="0" pageNumber="113">Habitat and distribution.</paragraph>
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<paragraph pageId="0" pageNumber="113">
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Isolated from leaves of
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<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Millettia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Millettia reticulata" order="Fabales" pageId="0" pageNumber="113" phylum="Tracheophyta" rank="species" species="reticulata">
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<emphasis italics="true" pageId="0" pageNumber="113">Millettia reticulata</emphasis>
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</taxonomicName>
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in China
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="113" type="etymology">
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<paragraph pageId="0" pageNumber="113">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="113">
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Species epithet
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<taxonomicName lsidName="millettiae" pageId="0" pageNumber="113" rank="species" species="millettiae">
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<emphasis italics="true" pageId="0" pageNumber="113">millettiae</emphasis>
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</taxonomicName>
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, referring to the host,
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<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Millettia" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Millettia reticulata" order="Fabales" pageId="0" pageNumber="113" phylum="Tracheophyta" rank="species" species="reticulata">
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<emphasis italics="true" pageId="0" pageNumber="113">Millettia reticulata</emphasis>
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</taxonomicName>
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from which the strain was isolated.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="113" type="notes">
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<paragraph pageId="0" pageNumber="113">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="113">
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Phylogenetic analysis combining four gene loci showed that
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<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe millettiae" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="millettiae">
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<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe millettiae</emphasis>
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</taxonomicName>
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(strain GUCC 9167) displayed a close relationship with
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<taxonomicName lsidName="D. arengae" pageId="0" pageNumber="113" rank="species" species="arengae">
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<emphasis italics="true" pageId="0" pageNumber="113">D. arengae</emphasis>
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</taxonomicName>
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,
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<taxonomicName lsidName="D. pseudomangiferae" pageId="0" pageNumber="113" rank="species" species="pseudomangiferae">
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<emphasis italics="true" pageId="0" pageNumber="113">D. pseudomangiferae</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="D. perseae" pageId="0" pageNumber="113" rank="species" species="perseae">
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<emphasis italics="true" pageId="0" pageNumber="113">D. perseae</emphasis>
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</taxonomicName>
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with high bootstrap values (
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<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Parsimonious tree obtained from a combined analyses of an ITS, β - tubulin, calmoudulin and tef 1 sequence dataset. MP, ML above 50 % and BPP values above 0.90 were placed close to topological nodes and separated by " / ". The bootstrap values below 50 % and BPP values below 0.90 were labelled with " - ". The tree is rooted with Valsa ambiens (CFCC 89894). The branch of our new Diaporthe species is in pink." figureDoi="10.3897/mycokeys.57.35448.figure1" httpUri="https://binary.pensoft.net/fig/328111" pageId="0" pageNumber="113">Figure 1</figureCitation>
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). We compared the DNA base pair differences of the four gene regions, the main differences being in the
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<emphasis italics="true" pageId="0" pageNumber="113">
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<normalizedToken originalValue="β">β</normalizedToken>
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</emphasis>
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-tubulin and
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<emphasis italics="true" pageId="0" pageNumber="113">tef</emphasis>
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1 genes, especially
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<emphasis italics="true" pageId="0" pageNumber="113">tef</emphasis>
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1.
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<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe millettiae" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="millettiae">
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<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe millettiae</emphasis>
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</taxonomicName>
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produced two types of conidia (α,
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<normalizedToken originalValue="β">β</normalizedToken>
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), whereas
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<taxonomicName lsidName="D. pseudomangiferae" pageId="0" pageNumber="113" rank="species" species="pseudomangiferae">
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<emphasis italics="true" pageId="0" pageNumber="113">D. pseudomangiferae</emphasis>
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</taxonomicName>
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only produced
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<emphasis italics="true" pageId="0" pageNumber="113">alpha conidia</emphasis>
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and
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<taxonomicName lsidName="D. perseae" pageId="0" pageNumber="113" rank="species" species="perseae">
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<emphasis italics="true" pageId="0" pageNumber="113">D. perseae</emphasis>
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</taxonomicName>
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produced three types of conidia (α,
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<normalizedToken originalValue="β">β</normalizedToken>
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,
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<normalizedToken originalValue="γ">γ</normalizedToken>
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). The
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<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
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of
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<taxonomicName lsidName="D. arengae" pageId="0" pageNumber="113" rank="species" species="arengae">
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<emphasis italics="true" pageId="0" pageNumber="113">D. arengae</emphasis>
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</taxonomicName>
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were smaller (20-25
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) than those of
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<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe millettiae" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="millettiae">
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<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe millettiae</emphasis>
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</taxonomicName>
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(17.5-32
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<normalizedToken originalValue="×">x</normalizedToken>
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1-2
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<normalizedToken originalValue="μm">μm</normalizedToken>
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). The shape of
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<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
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was also different. Conidiophores of
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<taxonomicName lsidName="D. arengae" pageId="0" pageNumber="113" rank="species" species="arengae">
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<emphasis italics="true" pageId="0" pageNumber="113">D. arengae</emphasis>
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</taxonomicName>
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(10-60
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) with more septa (0-6), were longer than those of
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<taxonomicName lsidName="D. millettiae" pageId="0" pageNumber="113" rank="species" species="millettiae">
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<emphasis italics="true" pageId="0" pageNumber="113">D. millettiae</emphasis>
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</taxonomicName>
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(10-23
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<normalizedToken originalValue="×">x</normalizedToken>
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1-2.5
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<normalizedToken originalValue="μm">μm</normalizedToken>
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; 0-1-septate) (
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<bibRefCitation DOI="https://doi.org/10.3767/003158513X666844" author="Gomes, RR" journalOrPublisher="Persoonia- Molecular Phylogeny and Evolution of Fungi" pageId="0" pageNumber="113" pagination="1 - 41" refId="B13" refString="Gomes, RR, Glienke, C, Videira, SIR, Lombard, L, Groenewald, JZ, Crous, PW, 2013. Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. . Persoonia- Molecular Phylogeny and Evolution of Fungi 31: 1 - 41" title="Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi." url="https://doi.org/10.3767/003158513X666844" volume="31" year="2013">Gomes et al. 2013</bibRefCitation>
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).
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</paragraph>
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<caption doi="10.3897/mycokeys.57.35448.figure3" httpUri="https://binary.pensoft.net/fig/328113" pageId="0" pageNumber="113" start="Figure 3" startId="F3">
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<paragraph pageId="0" pageNumber="113">
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Figure 3.
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<taxonomicName class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Diaporthe millettiae" order="Diaporthales" pageId="0" pageNumber="113" phylum="Ascomycota" rank="species" species="millettiae">
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<emphasis italics="true" pageId="0" pageNumber="113">Diaporthe millettiae</emphasis>
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</taxonomicName>
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(GUCC9167).
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<emphasis bold="true" pageId="0" pageNumber="113">
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<normalizedToken originalValue="a–b">a-b</normalizedToken>
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</emphasis>
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upper (
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<emphasis bold="true" pageId="0" pageNumber="113">a</emphasis>
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) and lower (
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<emphasis bold="true" pageId="0" pageNumber="113">b</emphasis>
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) surface of colony on PDA
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<emphasis bold="true" pageId="0" pageNumber="113">
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<normalizedToken originalValue="c–d">c-d</normalizedToken>
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</emphasis>
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conidiomata
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<emphasis bold="true" pageId="0" pageNumber="113">
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<normalizedToken originalValue="e–f">e-f</normalizedToken>
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</emphasis>
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conidiophores, conidiogenous loci and conidia
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<emphasis bold="true" pageId="0" pageNumber="113">g</emphasis>
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<normalizedToken originalValue="β-conidia">β-conidia</normalizedToken>
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<emphasis bold="true" pageId="0" pageNumber="113">h</emphasis>
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<normalizedToken originalValue="α-conidia">α-conidia</normalizedToken>
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. Scale bars: 20
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="113">e, f</emphasis>
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), 10
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<normalizedToken originalValue="µm">µm</normalizedToken>
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(
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<emphasis bold="true" pageId="0" pageNumber="113">g, h</emphasis>
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).
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</paragraph>
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</caption>
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</subSubSection>
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</treatment>
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</document> |