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<mods:title id="A3EE9DF99DBC6BAFF4F5D1145CB9849C">Phylogeography of the endemic red-tailed cicadas of New Zealand (Hemiptera: Cicadidae: Rhodopsalta), and molecular, morphological and bioacoustical confirmation of the existence of Hudsons Rhodopsalta microdora</mods:title>
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<mods:namePart id="BC43A0515A0349FA0421C453C107654B">Hill, Kathy B R</mods:namePart>
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<paragraph id="8BEB36EBFFE5FFE785A2C82C91C0BE50" blockId="9.[163,444,1813,1837]" box="[163,444,1813,1837]" pageId="9" pageNumber="1228">
<taxonomicName id="4C544D68FFE5FFE785A2C82C91C0BE50" ID-CoL="4SJCX" baseAuthorityName="Fabricius" baseAuthorityYear="1775" box="[163,444,1813,1837]" class="Insecta" family="Cicadidae" genus="Rhodopsalta" kingdom="Animalia" order="Hemiptera" pageId="9" pageNumber="1228" phylum="Arthropoda" rank="species" species="cruentata">Rhodopsalta cruentata</taxonomicName>
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<paragraph id="8BEB36EBFFE5FFE485A2C80794C4BE1B" blockId="9.[163,779,1853,1906]" lastBlockId="10.[809,1425,1812,1896]" lastPageId="10" lastPageNumber="1229" pageId="9" pageNumber="1228">
A sister-group relationship is found between NI and SI
<taxonomicName id="4C544D68FFE5FFE785C5C864912FBE0E" baseAuthorityName="Fabricius" baseAuthorityYear="1775" box="[196,339,1885,1906]" class="Insecta" family="Cicadidae" genus="Rhodopsalta" kingdom="Animalia" order="Hemiptera" pageId="9" pageNumber="1228" phylum="Arthropoda" rank="species" species="cruentata">
<emphasis id="B920EAF9FFE5FFE785C5C864912FBE0E" box="[196,339,1885,1906]" italics="true" pageId="9" pageNumber="1228">R. cruentata</emphasis>
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, with the exception of two
<collectingRegion id="4990F809FFE5FFE7878CC8659377BE0E" box="[653,779,1884,1906]" country="New Zealand" name="Wellington" pageId="9" pageNumber="1228">Wellington</collectingRegion>
specimens that group within the SI clade (
<figureCitation id="136F2A6EFFE5FFE78051C99A95E0BFC4" box="[1360,1436,1699,1721]" captionStart="Figure 4" captionStartId="9.[164,244,1521,1543]" captionTargetBox="[163,1444,195,1481]" captionTargetId="figure-119@9.[154,1459,189,1494]" captionTargetPageId="9" captionText="Figure 4. Bayesian phylogenetic tree based on 2284 bp of concatenated genes (5 and 3 cox1 and EF1α). Six partition subsets were used (by gene and codon position for each gene, respectively). Branch support values represent Bayesian posterior probabilities and RAXML bootstrap percentages from 1000 non-parametric pseudoreplicates. The number of parsimony-informative sites is 300. Grey boxes show mean divergence time estimates and 95% confidence intervals from the *BEAST analysis." figureDoi="http://doi.org/10.5281/zenodo.6985725" httpUri="https://zenodo.org/record/6985725/files/figure.png" pageId="9" pageNumber="1228">Figs 4</figureCitation>
,
<figureCitation id="136F2A6EFFE5FFE7863AC9F89327BFAB" box="[827,859,1729,1751]" captionStart="Figure 5" captionStartId="10.[145,223,1675,1697]" captionTargetBox="[255,1314,195,1636]" captionTargetId="graphics-72@10.[314,1314,195,1635]" captionTargetPageId="10" captionText="Figure 5. Single-gene Bayesian phylogenetic trees based on mitochondrial cox1 (1486 bp; A) and nuclear EF1α (798 bp; B) using CIPRES v.3.3. Branch support values represent Bayesian posterior probabilities and RAXML bootstrap percentages from 1000 non-parametric pseudoreplicates. The number of parsimony informative sites is 239 for cox1 and 61 for EF1α." figureDoi="http://doi.org/10.5281/zenodo.6985727" httpUri="https://zenodo.org/record/6985727/files/figure.png" pageId="9" pageNumber="1228">5A</figureCitation>
). These relationships are informed primarily by the
<emphasis id="B920EAF9FFE5FFE78668C9D993E7BF89" box="[873,923,1760,1781]" italics="true" pageId="9" pageNumber="1228">cox1</emphasis>
gene. Some weak geographical concordance is present. The earliest splits in the NI
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<emphasis id="B920EAF9FFE5FFE78013C9C695DFBE68" box="[1298,1443,1791,1812]" italics="true" pageId="9" pageNumber="1228">R. cruentata</emphasis>
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clade divide a set of samples from north-eastern NI from the remainder, and the majority of south-eastern NI individuals, specifically from
<collectingRegion id="4990F809FFE5FFE781CBC862951DBE0C" box="[1226,1377,1883,1905]" country="New Zealand" name="Hawke's Bay" pageId="9" pageNumber="1228">Hawkes Bay</collectingRegion>
(HB) and Rangitikei (RI), form a weakly supported subclade distinct from the rest of the NI specimens. Within the mainly SI
<taxonomicName id="4C544D68FFE6FFE4843FC86A91ADBE14" baseAuthorityName="Fabricius" baseAuthorityYear="1775" box="[318,465,1875,1896]" class="Insecta" family="Cicadidae" genus="Rhodopsalta" kingdom="Animalia" order="Hemiptera" pageId="10" pageNumber="1229" phylum="Arthropoda" rank="species" species="cruentata">
<emphasis id="B920EAF9FFE6FFE4843FC86A91ADBE14" box="[318,465,1875,1896]" italics="true" pageId="10" pageNumber="1229">R. cruentata</emphasis>
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clade, the earliest splits separate samples from the northern part of the range of the clade [
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(WN),
<collectingRegion id="4990F809FFE6FFE48198C80A954EBE35" box="[1177,1330,1843,1865]" country="New Zealand" name="Marlborough" pageId="10" pageNumber="1229">Marlborough</collectingRegion>
Sounds (SD) and northern Kaikoura (KA)].
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