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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.56.36079" ID-GBIF-Dataset="50ed5503-499b-4b41-8e64-9dff5230e604" ID-PMC="PMC6637038" ID-Pensoft-Pub="1314-4049-56-49" ID-PubMed="31341399" ModsDocAuthor="" ModsDocDate="2019" ModsDocID="1314-4049-56-49" ModsDocOrigin="MycoKeys 56" ModsDocTitle="Nectria-related fungi causing dieback and canker diseases in China, with Neothyronectriacitri sp. nov. described" checkinTime="1562806757137" checkinUser="pensoft" docAuthor="Yang, Qin, Chen, Wen-Yan, Jiang, Ning &amp; Tian, Cheng-Ming" docDate="2019" docId="E51DC920FC26AC7882D9FD0DB6B5ED18" docLanguage="en" docName="MycoKeys 56: 49-66" docOrigin="MycoKeys 56" docSource="http://dx.doi.org/10.3897/mycokeys.56.36079" docTitle="Neothyronectria citri C. M. Tian &amp; Q. Yang, sp. nov." docType="treatment" docVersion="4" lastPageNumber="49" masterDocId="1276872AFFAFFFDCBB64FFFCFF8CD962" masterDocTitle="Nectria-related fungi causing dieback and canker diseases in China, with Neothyronectriacitri sp. nov. described" masterLastPageNumber="66" masterPageNumber="49" pageNumber="49" updateTime="1668136283647" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Nectria-related fungi causing dieback and canker diseases in China, with Neothyronectriacitri sp. nov. described</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Yang, Qin</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Chen, Wen-Yan</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Jiang, Ning</mods:namePart>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Tian, Cheng-Ming</mods:namePart>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2019</mods:date>
<mods:detail type="volume">
<mods:number>56</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>49</mods:start>
<mods:end>66</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.56.36079</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.56.36079</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-56-49</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="158599587" LSID="urn:lsid:plazi:treatment:E51DC920FC26AC7882D9FD0DB6B5ED18" httpUri="http://treatment.plazi.org/id/E51DC920FC26AC7882D9FD0DB6B5ED18" lastPageNumber="49" pageId="0" pageNumber="49">
<subSubSection pageId="0" pageNumber="49" type="nomenclature">
<paragraph pageId="0" pageNumber="49">
<taxonomicName LSID="830779" authority="C. M. Tian &amp; Q. Yang" class="Sordariomycetes" family="Nectriaceae" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria citri" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="citri">Neothyronectria citri C.M. Tian &amp; Q. Yang</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="49">sp. nov.</taxonomicNameLabel>
Figure 4
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="49" type="diagnosis">
<paragraph pageId="0" pageNumber="49">Diagnosis.</paragraph>
<paragraph pageId="0" pageNumber="49">
<taxonomicName class="Sordariomycetes" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria citri" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="citri">Neothyronectria citri</taxonomicName>
differs from its closest phylogenetic neighbour
<taxonomicName class="Sordariomycetes" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria sophorae" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="sophorae">Neothyronectria sophorae</taxonomicName>
in ITS, LSU and tub2 loci, based on the alignments deposited in TreeBASE.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="49" type="holotype">
<paragraph pageId="0" pageNumber="49">Holotype.</paragraph>
<paragraph pageId="0" pageNumber="49">
CHINA. Jiangxi Province: Ganzhou city,
<geoCoordinate direction="north" orientation="latitude" precision="1" value="25.857742">25°51'27.87&quot;N</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="1" value="114.97193">114°58'18.95&quot;E</geoCoordinate>
, on symptomatic branches of
<taxonomicName class="Magnoliopsida" family="Rutaceae" genus="Citrus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Citrus maxima" order="Sapindales" pageId="0" pageNumber="49" phylum="Tracheophyta" rank="species" species="maxima">Citrus maxima</taxonomicName>
(Burm.) Merr. cv. Shatian Yu, 11 May 2018, Q. Yang, Y.M. Liang &amp; Y. Liu (holotype BJFC-S1770 designated here, ex-type culture CFCC 53590).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="49" type="etymology">
<paragraph pageId="0" pageNumber="49">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="49">
Named after the host genus on which it was collected,
<taxonomicName class="Magnoliopsida" family="Rutaceae" genus="Citrus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Citrus" order="Sapindales" pageId="0" pageNumber="49" phylum="Tracheophyta" rank="genus">Citrus</taxonomicName>
.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="49" type="description">
<paragraph pageId="0" pageNumber="49">Description.</paragraph>
<paragraph pageId="0" pageNumber="49">
Mycelium not visible around ascomata or on the host. Stromata erumpent through epidermis, up to 0.6 mm high and 1 mm diam., pseudoparenchymatous, cells forming textura angularis to t. globulosa, intergrading with ascomatal wall. Ascomata superficial on well-developed stromata, scattered to aggregated in groups of 3-10, subglobose to globose, 200-270
<normalizedToken originalValue="μm">μm</normalizedToken>
diam., rarely slightly cupulate upon drying, sometimes with only a depressed apical region, yellowish-brown to grey, apical region slightly darker, no colour change in KOH or LA, sometimes surface scurfy or scaly, bright yellow to greenish-yellow. Ascomatal surface cells forming textura globulosa or t. angularis, sometimes including bright yellow scurf, 9-15
<normalizedToken originalValue="μm">μm</normalizedToken>
diam., walls pigmented, uniformly about 1.5
<normalizedToken originalValue="μm">μm</normalizedToken>
thick. Ascomatal wall 27-46
<normalizedToken originalValue="μm">μm</normalizedToken>
thick, of two regions: outer region 22-35
<normalizedToken originalValue="μm">μm</normalizedToken>
thick, intergrading with stroma, cells forming textura globulosa or t. angularis, walls pigmented, about 1.5
<normalizedToken originalValue="μm">μm</normalizedToken>
thick; inner region 9-15
<normalizedToken originalValue="μm">μm</normalizedToken>
thick, of elongate, thin-walled, hyaline cells, forming textura prismatica. Asci clavate, unitunicate, 53.5-65
<normalizedToken originalValue="×">x</normalizedToken>
8.5-11
<normalizedToken originalValue="μm">μm</normalizedToken>
, with inconspicuous ring at apex, 4-spored. Ascospores allantoid to short-cylindrical, uniseriate, rounded at both ends, (17
<normalizedToken originalValue=")1821(">-)18-21(-</normalizedToken>
23.5)
<normalizedToken originalValue="×">x</normalizedToken>
8
<normalizedToken originalValue="9(">-9(-</normalizedToken>
10)
<normalizedToken originalValue="μm">μm</normalizedToken>
(n = 20), muriform, hyaline to slightly yellowish-brown.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="49" type="description">
<paragraph pageId="0" pageNumber="49">Culture characters.</paragraph>
<paragraph pageId="0" pageNumber="49">Cultures incubated on PDA at 25 °C in darkness. Colony originally flat with white aerial mycelium, becoming pale yellowish due to pigment formation, conidiomata absent.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="49" type="additional specimen examined">
<paragraph pageId="0" pageNumber="49">Additional specimen examined.</paragraph>
<paragraph pageId="0" pageNumber="49">
CHINA. Jiangxi Province: Ganzhou City,
<geoCoordinate direction="north" orientation="latitude" precision="1" value="25.857742">25°51'27.87&quot;N</geoCoordinate>
,
<geoCoordinate direction="east" orientation="longitude" precision="1" value="114.97193">114°58'18.95&quot;E</geoCoordinate>
, on symptomatic branches of
<taxonomicName class="Magnoliopsida" family="Rutaceae" genus="Citrus" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Citrus maxima" order="Sapindales" pageId="0" pageNumber="49" phylum="Tracheophyta" rank="species" species="maxima">Citrus maxima</taxonomicName>
(Burm.) Merr. cv. Shatian Yu, 11 May 2018, Q. Yang, Y.M. Liang &amp; Y. Liu (BJFC-S1771, living culture CFCC 53591).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="49" type="notes">
<paragraph pageId="0" pageNumber="49">Note.</paragraph>
<paragraph pageId="0" pageNumber="49">
<taxonomicName class="Sordariomycetes" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria citri" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="citri">Neothyronectria citri</taxonomicName>
, as described here, is known from an ascomatal sexual morph phylogenetically allied to species of
<taxonomicName class="Sordariomycetes" genus="Allantonectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Allantonectria" pageId="0" pageNumber="49" phylum="Ascomycota" rank="genus">Allantonectria</taxonomicName>
and
<taxonomicName class="Sordariomycetes" family="Thyridiaceae" genus="Thyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Thyronectria" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="genus">Thyronectria</taxonomicName>
(Fig. 1). In this study, two strains representing
<taxonomicName class="Sordariomycetes" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria citri" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="citri">Neothyronectria citri</taxonomicName>
cluster in a well-supported clade and appear most closely related to
<taxonomicName class="Sordariomycetes" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria sophorae" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="sophorae">Neothyronectria sophorae</taxonomicName>
, which was isolated from
<taxonomicName class="Magnoliopsida" family="Fabaceae" genus="Sophora" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Sophora microphylla" order="Fabales" pageId="0" pageNumber="49" phylum="Tracheophyta" rank="species" species="microphylla">Sophora microphylla</taxonomicName>
in New Zealand (
<bibRefCitation author="Crous, PW" journalOrPublisher="Persoonia" pageId="0" pageNumber="49" pagination="218 - 403" title="Fungal Planet description sheets: 469 - 557." url="https://doi.org/10.3767/003158516X694499" volume="37" year="2016">Crous et al. 2016</bibRefCitation>
).
<taxonomicName class="Sordariomycetes" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria citri" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="citri">Neothyronectria citri</taxonomicName>
can be distinguished, based on ITS, LSU and tub2 loci from
<taxonomicName class="Sordariomycetes" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria sophorae" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="sophorae">Neothyronectria sophorae</taxonomicName>
(16/464 in ITS, 9/772 in LSU and 60/494 in tub2).
</paragraph>
<caption pageId="0" pageNumber="49">
<paragraph pageId="0" pageNumber="49">
Figure 4.
<taxonomicName class="Sordariomycetes" genus="Neothyronectria" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Neothyronectria citri" order="Hypocreales" pageId="0" pageNumber="49" phylum="Ascomycota" rank="species" species="citri">Neothyronectria citri</taxonomicName>
(CFCC 53590)
<normalizedToken originalValue="AB">A-B</normalizedToken>
habit of conidiomata on branches C transverse section of conidioma D longitudinal section of conidioma
<normalizedToken originalValue="EF">E-F</normalizedToken>
asci
<normalizedToken originalValue="GH">G-H</normalizedToken>
ascospores. Scale bars: 500
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="BD">B-D</normalizedToken>
); 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<normalizedToken originalValue="EH">E-H</normalizedToken>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>