322 lines
66 KiB
XML
322 lines
66 KiB
XML
<document id="3B2813F40DF2EC5B07EE21D2DFC9DE6E" ID-CLB-Dataset="46229" ID-DOI="10.5281/zenodo.201653" ID-GBIF-Dataset="8a0e568c-6635-48db-97fb-228ef967a81f" ID-ISSN="1175-5326" ID-Zenodo-Dep="201653" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1460396965546" checkinUser="plazi" docAuthor="Chen, Ruimin, Lin, Xiaofeng & Warren, Alan" docDate="2011" docId="B85487AF5F2B0314FF287CFF1CEDFB5E" docLanguage="en" docName="zt03048p068.pdf" docOrigin="Zootaxa 3048" docStyle="DocumentStyle:890A69B780ED73D6DB8551B71C8AC79E.4:Zootaxa.2009-2012.journal_article" docStyleId="890A69B780ED73D6DB8551B71C8AC79E" docStyleName="Zootaxa.2009-2012.journal_article" docStyleVersion="4" docTitle="Amphileptus salignus Chen, Lin & Warren, 2011, n. sp." docType="treatment" docVersion="8" lastPageNumber="65" masterDocId="446DFFD75F2A0317FFBF7D431843FFE7" masterDocTitle="A new pleurostomatid ciliate, Amphileptus salignus n. sp. (Protozoa, Ciliophora), from mangrove wetlands in southern China" masterLastPageNumber="68" masterPageNumber="62" pageNumber="63" updateTime="1698298780247" updateUser="ExternalLinkService">
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<mods:title id="E2B45CA1F41F4571A2FA91EE0242E54B">A new pleurostomatid ciliate, Amphileptus salignus n. sp. (Protozoa, Ciliophora), from mangrove wetlands in southern China</mods:title>
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<mods:name id="81193051DDDFDFFC35E824FC9FE76D73" type="personal">
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<mods:namePart id="0411341C47E4B785FD399CAEE5ED3843">Chen, Ruimin</mods:namePart>
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<mods:namePart id="A31467F9B4710D59E7D995152828A46E">Lin, Xiaofeng</mods:namePart>
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<mods:namePart id="8065FABF6A12EFAC11F608DAA8517245">Warren, Alan</mods:namePart>
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<mods:typeOfResource id="2BF56C6DE2A52AB06EA305506E0D1040">text</mods:typeOfResource>
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<mods:title id="CF1830F3402F74FC4F434C77C5F076F9">Zootaxa</mods:title>
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<mods:part id="C14F9B596F5E37C382D23C51F978CE73">
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<mods:date id="ABA303323A7A68B41B16EA5949A9EC60">2011</mods:date>
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<mods:number id="60487DAA9B75DD61A3475ED73F836C74">3048</mods:number>
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<mods:identifier id="A60696C6EE4EEBB54542891BE40E5305" type="DOI">10.5281/zenodo.201653</mods:identifier>
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<treatment id="B85487AF5F2B0314FF287CFF1CEDFB5E" ID-DOI="http://doi.org/10.5281/zenodo.5662306" ID-GBIF-Taxon="119418245" ID-Zenodo-Dep="5662306" LSID="urn:lsid:plazi:treatment:B85487AF5F2B0314FF287CFF1CEDFB5E" httpUri="http://treatment.plazi.org/id/B85487AF5F2B0314FF287CFF1CEDFB5E" lastPageId="3" lastPageNumber="65" pageId="1" pageNumber="63">
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<subSubSection id="78E765325F2B0316FF287CFF19C2FE1F" pageId="1" pageNumber="63" type="nomenclature">
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<paragraph id="304236B95F2B0316FF287CFF19A5FE32" blockId="1.[151,486,443,504]" box="[151,486,443,470]" pageId="1" pageNumber="63">
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<heading id="6B0A81D55F2B0316FF287CFF19A5FE32" bold="true" box="[151,486,443,470]" fontSize="11" level="1" pageId="1" pageNumber="63" reason="1">
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<emphasis id="0289EAAB5F2B0316FF287CFF19A5FE32" bold="true" box="[151,486,443,470]" pageId="1" pageNumber="63">
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<taxonomicName id="F7FD4D3A5F2B0316FF287CFF19DFFE31" box="[151,412,444,470]" class="Gymnostomatea" family="Amphileptidae" genus="Amphileptus" kingdom="Protozoa" order="Pleurostomatida" pageId="1" pageNumber="63" phylum="Ciliophora" rank="species" species="salignus" status="sp. nov.">
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<emphasis id="0289EAAB5F2B0316FF287CFF19DFFE31" bold="true" box="[151,412,444,470]" italics="true" pageId="1" pageNumber="63">Amphileptus salignus</emphasis>
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</taxonomicName>
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<taxonomicNameLabel id="19BA57D05F2B0316FE1B7CF819A5FE32" box="[420,486,443,469]" pageId="1" pageNumber="63" rank="species">n. sp.</taxonomicNameLabel>
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</emphasis>
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</heading>
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</paragraph>
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<paragraph id="304236B95F2B0316FF287CA319C2FE1F" blockId="1.[151,486,443,504]" box="[151,385,480,504]" pageId="1" pageNumber="63">
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(
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<figureCitation id="A8C62A3C5F2B0316FF207CA3195AFE1F" box="[159,281,480,504]" captionStart-0="FIGURES 1 – 9" captionStart-1="FIGURES 10 – 23" captionStartId-0="2.[151,264,1634,1657]" captionStartId-1="4.[151,264,1496,1519]" captionTargetBox-0="[168,1428,200,1590]" captionTargetBox-1="[151,1436,429,1442]" captionTargetId-0="figure@2.[151,1436,195,1595]" captionTargetId-1="figure@4.[151,1436,429,1443]" captionTargetPageId-0="2" captionTargetPageId-1="4" captionText-0="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." captionText-1="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri-0="https://zenodo.org/record/201654/files/figure.png" httpUri-1="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">Figs. 1–23</figureCitation>
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;
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<tableCitation id="7D7F03025F2B0316FE9A7CA31935FE1F" box="[293,374,480,504]" captionStart="TABLE 1" captionStartId="3.[151,235,152,173]" captionText="TABLE 1. Morphometric data based on two populations of Amphileptus salignus n. sp. (upper rows, Shenzhen population; lower rows, Hong Kong population). All data are based on protargol impregnated specimens." pageId="1" pageNumber="63">Table 1</tableCitation>
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)
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</paragraph>
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</subSubSection>
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<subSubSection id="78E765325F2B0316FF287F6A1C92FD48" pageId="1" pageNumber="63" type="diagnosis">
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<paragraph id="304236B95F2B0316FF287F6A1C92FD48" blockId="1.[151,1437,552,1929]" pageId="1" pageNumber="63">
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<emphasis id="0289EAAB5F2B0316FF287F6A1952FDA5" bold="true" box="[151,273,553,578]" pageId="1" pageNumber="63">Diagnosis.</emphasis>
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Large brackish water
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<taxonomicName id="F7FD4D3A5F2B0316FDA87F691AE0FDA5" box="[535,675,554,578]" class="Gymnostomatea" family="Amphileptidae" genus="Amphileptus" kingdom="Protozoa" order="Pleurostomatida" pageId="1" pageNumber="63" phylum="Ciliophora" rank="genus">
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<emphasis id="0289EAAB5F2B0316FDA87F691AE0FDA5" box="[535,675,554,578]" italics="true" pageId="1" pageNumber="63">Amphileptus</emphasis>
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</taxonomicName>
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180–360 μm long
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<emphasis id="0289EAAB5F2B0316FC3B7F681B90FDA5" box="[900,979,555,578]" italics="true" pageId="1" pageNumber="63">in vivo</emphasis>
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; lanceolate body shape; two ellipsoidal macronuclei and one micronucleus; 24–29 right and 4 left somatic kineties; several contractile vacuoles positioned ventrally in posterior 3/4 of cell and usually one near dorsal margin; two
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<typeStatus id="EF46881B5F2B0316FC6A7F371C53FD6B" box="[981,1040,628,652]" pageId="1" pageNumber="63">types</typeStatus>
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of extrusomes, the longer one barshaped and mostly arranged along the oral slit, the other short-bar-like packed under the pellicle.
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</paragraph>
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</subSubSection>
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<subSubSection id="78E765325F2B0316FF7A7FFF1CE8FCA6" pageId="1" pageNumber="63" type="materials_examined">
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||
<paragraph id="304236B95F2B0316FF7A7FFF1C81FCFA" blockId="1.[151,1437,552,1929]" pageId="1" pageNumber="63">
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||
<emphasis id="0289EAAB5F2B0316FF7A7FFF190EFD33" bold="true" box="[197,333,699,724]" pageId="1" pageNumber="63">
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||
<typeStatus id="EF46881B5F2B0316FF7A7FFF18BDFD33" box="[197,254,700,724]" pageId="1" pageNumber="63">Type</typeStatus>
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slides.
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</emphasis>
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The
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<typeStatus id="EF46881B5F2B0316FE367FFF19A8FD33" box="[393,491,700,724]" pageId="1" pageNumber="63" type="holotype">holotype</typeStatus>
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slide (registration number: CRM
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<date id="444310795F2B0316FCDA7FFF1BF8FD33" box="[869,955,700,724]" pageId="1" pageNumber="63" value="01-09-04">090401</date>
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-01) of protargol-impregnated specimens is deposited in the Laboratory of Protozoology, South
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<collectingCountry id="48EA76295F2B0316FD607FA31B61FD1F" box="[735,802,736,760]" name="China" pageId="1" pageNumber="63">China</collectingCountry>
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Normal University,
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<collectingCountry id="48EA76295F2B0316FBB67FA31C13FD1F" box="[1033,1104,736,760]" name="China" pageId="1" pageNumber="63">China</collectingCountry>
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. One
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<typeStatus id="EF46881B5F2B0316FB2E7FA21CB1FD1E" box="[1169,1266,737,761]" pageId="1" pageNumber="63" type="paratype">paratype</typeStatus>
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slide (registration number:
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<collectionCode id="56ECAE7C5F2B0316FE917E4619E6FCFA" LSID="urn:lsid:biocol.org:col:34665" box="[302,421,773,797]" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34665" name="Natural History Museum, London" pageId="1" pageNumber="63">NHMUK</collectionCode>
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2010:11:4:1) is deposited in the Natural History Museum, London,
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<collectingCountry id="48EA76295F2B0316FB347E451C81FCFA" box="[1163,1218,774,797]" name="United Kingdom" pageId="1" pageNumber="63">U.K.</collectingCountry>
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</paragraph>
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||
<paragraph id="304236B95F2B0316FF7A7E691CE8FCA6" blockId="1.[151,1437,552,1929]" box="[197,1195,809,834]" pageId="1" pageNumber="63">
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<emphasis id="0289EAAB5F2B0316FF7A7E691920FCA5" bold="true" box="[197,355,809,834]" pageId="1" pageNumber="63">
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<typeStatus id="EF46881B5F2B0316FF7A7E691943FCA5" box="[197,256,810,834]" pageId="1" pageNumber="63">Type</typeStatus>
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locality.
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</emphasis>
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Futian mangrove wetland in Shenzhen (
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<geoCoordinate id="55C9507E5F2B0316FC977E6A1BF3FCA6" box="[808,944,809,833]" direction="north" orientation="latitude" pageId="1" pageNumber="63" precision="15" value="22.530277">22°31'49"N</geoCoordinate>
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;
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<geoCoordinate id="55C9507E5F2B0316FC037E6A1C0FFCA6" box="[956,1100,809,833]" direction="east" orientation="longitude" pageId="1" pageNumber="63" precision="15" value="113.99583">113°59'45"E</geoCoordinate>
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),
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<collectingCountry id="48EA76295F2B0316FBDE7E6A1CEBFCA6" box="[1121,1192,809,833]" name="China" pageId="1" pageNumber="63">China</collectingCountry>
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.
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</paragraph>
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</subSubSection>
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<subSubSection id="78E765325F2B0314FF7A7E0D1CEDFB5E" lastPageId="3" lastPageNumber="65" pageId="1" pageNumber="63" type="etymology">
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<paragraph id="304236B95F2B0316FF7A7E0D1D74FC81" blockId="1.[151,1437,552,1929]" box="[197,1335,846,870]" pageId="1" pageNumber="63">
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<emphasis id="0289EAAB5F2B0316FF7A7E0D1908FC81" bold="true" box="[197,331,846,870]" pageId="1" pageNumber="63">Etymology.</emphasis>
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The Latin word
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<taxonomicName id="F7FD4D3A5F2B0316FDB87E0D1A26FC81" box="[519,613,846,870]" class="Gymnostomatea" family="Amphileptidae" genus="Amphileptus" kingdom="Protozoa" order="Pleurostomatida" pageId="1" pageNumber="63" phylum="Ciliophora" rank="species" species="salignus">
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<emphasis id="0289EAAB5F2B0316FDB87E0D1A26FC81" box="[519,613,846,870]" italics="true" pageId="1" pageNumber="63">salignus</emphasis>
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</taxonomicName>
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refers to the cell shape which is like the leaf of a willow (
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<taxonomicName id="F7FD4D3A5F2B0316FB507E0D1D64FC81" box="[1263,1319,846,870]" class="Ascidiacea" family="Polycitoridae" genus="Salix" kingdom="Animalia" order="Enterogona" pageId="1" pageNumber="63" phylum="Chordata" rank="genus">
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<emphasis id="0289EAAB5F2B0316FB507E0D1D64FC81" box="[1263,1319,846,870]" italics="true" pageId="1" pageNumber="63">Salix</emphasis>
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</taxonomicName>
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).
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</paragraph>
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<paragraph id="304236B95F2B0316FF7A7E311C0DFB81" blockId="1.[151,1437,552,1929]" pageId="1" pageNumber="63">
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<emphasis id="0289EAAB5F2B0316FF7A7E311A49FC6C" bold="true" box="[197,522,882,907]" pageId="1" pageNumber="63">Morphological description.</emphasis>
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Cells size highly variable, 180–360 × 55–65 μm
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<emphasis id="0289EAAB5F2B0316FB817E371CC9FC6C" box="[1086,1162,884,907]" italics="true" pageId="1" pageNumber="63">in vivo</emphasis>
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, mostly 200–300 μm in length. Body slightly contractile, shaped like the leaf of willow with posterior end bluntly pointed and neck region, which is about 20% of body length, usually curved slightly to dorsal side (
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<figureCitation id="A8C62A3C5F2B0316FC667EF81C22FC34" box="[985,1121,955,979]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 1, 4, 5</figureCitation>
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,
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<figureCitation id="A8C62A3C5F2B0316FBD27EF81CF7FC34" box="[1133,1204,955,979]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">10, 11</figureCitation>
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). Cell laterally compressed about 3 to 4:1; right side flat, left side slightly vaulted in mid-body region and with four longitudinal furrows that are conspicuous at high magnifications (
|
||
<figureCitation id="A8C62A3C5F2B0316FD6479471B6CFBFB" box="[731,815,1028,1052]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">Fig. 11</figureCitation>
|
||
). Right side densely ciliated, cilia 8–10 μm in length, ciliary rows forming a distinct anterior suture that is detectable
|
||
<emphasis id="0289EAAB5F2B0316FCD879691BF0FBA6" box="[871,947,1066,1089]" italics="true" pageId="1" pageNumber="63">in vivo</emphasis>
|
||
. Left somatic cilia sparsely distributed and difficult to detect
|
||
<emphasis id="0289EAAB5F2B0316FEE1790C19E9FB81" box="[350,426,1103,1126]" italics="true" pageId="1" pageNumber="63">in vivo</emphasis>
|
||
: dorsal brush conspicuous, cilia about 2–3 μm long (
|
||
<figureCitation id="A8C62A3C5F2B0316FC44790D1C03FB81" box="[1019,1088,1102,1126]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Fig. 5</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="304236B95F2B0316FF7A79301CABFA49" blockId="1.[151,1437,552,1929]" pageId="1" pageNumber="63">
|
||
Numerous tiny (<0.5 μm across), colourless dot-like cortical granules densely packed beneath pellicle between ciliary rows on both sides of cell (
|
||
<figureCitation id="A8C62A3C5F2B0316FDA679D41A2AFB48" box="[537,617,1175,1199]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 3</figureCitation>
|
||
,
|
||
<figureCitation id="A8C62A3C5F2B0316FDC979D41AD6FB48" box="[630,661,1175,1199]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">18</figureCitation>
|
||
). Cytoplasm colourless to slightly brown-grayish, often with numerous shining globules (1–2 μm across), which render main part of body more or less opaque, especially at low magnifications (
|
||
<figureCitation id="A8C62A3C5F2B0316FEA1799C19CEFB10" box="[286,397,1247,1271]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 1, 5</figureCitation>
|
||
,
|
||
<figureCitation id="A8C62A3C5F2B0316FE23799C19F8FB10" box="[412,443,1247,1271]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">10</figureCitation>
|
||
). Two kinds of extrusomes (
|
||
<figureCitation id="A8C62A3C5F2B0316FCBB799C1B16FB10" box="[772,853,1247,1271]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 2</figureCitation>
|
||
,
|
||
<figureCitation id="A8C62A3C5F2B0316FCDC799C1C44FB10" box="[867,1031,1247,1271]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">16, 17, 21–23</figureCitation>
|
||
):
|
||
<typeStatus id="EF46881B5F2B0316FBA379A31C0FFB1F" box="[1052,1100,1248,1272]" pageId="1" pageNumber="63">type</typeStatus>
|
||
<date id="444310795F2B0316FBEA79A31CE3FB10" box="[1109,1184,1247,1271]" pageId="1" pageNumber="63" value="1918-01-08">I 8–18</date>
|
||
μm long
|
||
<emphasis id="0289EAAB5F2B0316FAAD79A21D1CFB1F" box="[1298,1375,1249,1272]" italics="true" pageId="1" pageNumber="63">in vivo</emphasis>
|
||
, barshaped, densely arranged in oral area and some distributed in cytoplasm (
|
||
<figureCitation id="A8C62A3C5F2B0316FC7278471C17FAFB" box="[973,1108,1284,1308]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 1, 2, 9</figureCitation>
|
||
,
|
||
<figureCitation id="A8C62A3C5F2B0316FBDE78471CBDFAFB" box="[1121,1278,1284,1308]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">10, 16, 17, 23</figureCitation>
|
||
);
|
||
<typeStatus id="EF46881B5F2B0316FAAC78461D00FAFA" box="[1299,1347,1285,1309]" pageId="1" pageNumber="63">type</typeStatus>
|
||
II short bar-like, about 2 μm long, packed under the pellicle and some scattered in cytoplasm (
|
||
<figureCitation id="A8C62A3C5F2B0316FBD8786A1CF4FAA6" box="[1127,1207,1321,1345]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 2</figureCitation>
|
||
,
|
||
<figureCitation id="A8C62A3C5F2B0316FB7C786A1D27FAA6" box="[1219,1380,1321,1345]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">16, 17, 21, 22</figureCitation>
|
||
), not observed in stained specimens. Food vacuoles few in number, about 4 μm across. Two to seven contractile vacuoles (CV), about 6–12 μm in diameter, largest usually subterminally located, one near dorsal margin at posterior part, the others distributed unevenly along ventral margin in posterior 3/4 of body (
|
||
<figureCitation id="A8C62A3C5F2B0316FC4078D51CC5FA49" box="[1023,1158,1430,1454]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 1, 4, 5</figureCitation>
|
||
,
|
||
<figureCitation id="A8C62A3C5F2B0316FB2D78D51C98FA49" box="[1170,1243,1430,1454]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">10, 19</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="304236B95F2B0316FF7A78FF1CFDF9FB" blockId="1.[151,1437,552,1929]" pageId="1" pageNumber="63">
|
||
Two spherical to ovoid macronuclear nodules located in mid-body, size 30–60 × 18–36 μm after fixation, often appearing as two large transparent areas
|
||
<emphasis id="0289EAAB5F2B0316FDD378A21AF9FA1F" box="[620,698,1505,1528]" italics="true" pageId="1" pageNumber="63">in vivo</emphasis>
|
||
(
|
||
<figureCitation id="A8C62A3C5F2B0316FD73789C1B7FFA10" box="[716,828,1503,1527]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 1, 5</figureCitation>
|
||
,
|
||
<figureCitation id="A8C62A3C5F2B0316FCF4789C1BD7FA10" box="[843,916,1503,1527]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">10, 11</figureCitation>
|
||
). Single spherical micronucleus between two macronuclear nodules, undetectable
|
||
<emphasis id="0289EAAB5F2B0316FD8F7B461A38F9FB" box="[560,635,1541,1564]" italics="true" pageId="1" pageNumber="63">in vivo</emphasis>
|
||
, about 3 μm in length after protargol impregnation.
|
||
</paragraph>
|
||
<paragraph id="304236B95F2B0316FF7A7B6A1D39F9A7" blockId="1.[151,1437,552,1929]" box="[197,1402,1576,1600]" pageId="1" pageNumber="63">Movement by swimming with moderately slow rotation around longitudinal axis or by gliding on substrate.</paragraph>
|
||
<paragraph id="304236B95F2B0316FF7A7B0E19F6F934" blockId="1.[151,1437,552,1929]" pageId="1" pageNumber="63">
|
||
Infraciliature typical of genus (
|
||
<figureCitation id="A8C62A3C5F2B0316FD987B0E1AD5F982" box="[551,662,1613,1637]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 6–8</figureCitation>
|
||
). Two densely ciliated perioral kineties (PK1, PK2): PK1 on left of oral slit, extending to posterior end of cell, about half of kinety length consisting of monokinetids and half dikinetids (
|
||
<figureCitation id="A8C62A3C5F2B0316FF557BD51915F949" box="[234,342,1686,1710]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 6, 8</figureCitation>
|
||
); PK2 right of oral slit, composed of closely spaced dikinetids in anterior half and monokinetids posterior half (
|
||
<figureCitation id="A8C62A3C5F2B0316FE837BF819E4F934" box="[316,423,1723,1747]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 6, 8</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="304236B95F2B0316FF7A7B9C1DD5F86E" blockId="1.[151,1437,552,1929]" pageId="1" pageNumber="63">
|
||
Right somatic kineties closely spaced, 24–29 (mean 27) including PK2, anterior end of each intermediate kinety shortened forming a distinct anterior suture (
|
||
<figureCitation id="A8C62A3C5F2B0316FD6C7A471B54F8FB" box="[723,791,1796,1820]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Fig. 7</figureCitation>
|
||
). Four densely ciliated left somatic kineties, including the left perioral kinety and the dorsal brush kinety, all of which reach anterior end of cell; dorsal brush extending 1/3 of cell-length and composed of regularly spaced dikinetids (
|
||
<figureCitation id="A8C62A3C5F2B0316FC8C7A0E1B38F882" box="[819,891,1869,1893]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Fig. 8</figureCitation>
|
||
). Well-developed nematodesmata, all of which originate from kinetosomes of right perioral kinety, extending along cytopharynx and into cytoplasm (
|
||
<figureCitation id="A8C62A3C5F2B0316FAB17A321D1EF86E" box="[1294,1373,1905,1929]" captionStart="FIGURES 1 – 9" captionStartId="2.[151,264,1634,1657]" captionTargetBox="[168,1428,200,1590]" captionTargetId="figure@2.[151,1436,195,1595]" captionTargetPageId="2" captionText="FIGURES 1 – 9. Amphileptus salignus n. sp. from living cells (1 – 5) and after protargol impregnation (6 – 9). (1) Right view of a typical specimen with pointed posterior end. (2) Two kinds of extrusomes. (3) Cortical granules distributed between ciliary rows. (4) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (5) Left view, note the dorsal brush. (6) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (7 – 8) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig. 7 mark the suture. (9) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK 1 – 2: perioral kineties 1 and 2. Scale bars in (1, 4) — 100 µm, in (2) – 10 µm, in (7, 8) — 80 µm." httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="1" pageNumber="63">Figs. 6</figureCitation>
|
||
,
|
||
<figureCitation id="A8C62A3C5F2B0316FAD67A321DCBF86E" box="[1385,1416,1905,1929]" captionStart="FIGURES 10 – 23" captionStartId="4.[151,264,1496,1519]" captionTargetBox="[151,1436,429,1442]" captionTargetId="figure@4.[151,1436,429,1443]" captionTargetPageId="4" captionText="FIGURES 10 – 23. Photomicrographs showing the morphology of Amphileptus salignus n. sp. from living cells (10 – 11, 16 – 22) and after protargol impregnation (12 – 15, 23). (10) Right view of a typical individual, arrows mark the extrusomes, arrowheads mark the contractile vacuoles. (11) Left view, arrowheads mark the longitudinal ridges. (12) To show the oral structure, arrowheads mark perioral kinety 1, arrow marks perioral kinety 2. (13) To show the nematodesmata (arrowheads). (14) Left view of anterior portion, arrows show the dorsal brush. (15) The distribution of macronuclear nodules (arrows) and long extrusomes (arrowheads). (16, 17) Two kinds of extrusomes, arrowheads show the shorter ones, arrows show the longer ones. (18) Cortical granules (arrowhead). (19) To show the contractile vacuoles, arrowhead marks the one positioned near the dorsal side. (20) To show dorsal cilia (arrowheads) and dorsal brush (arrow). (21) Anterior region of cell, arrowheads point to the shorter extrusomes. (22) Mid-region of cell, arrowheads indicate the shorter extrusomes. (23) To show the longer extrusomes. Scale bars in (10, 11) — 100 µm, in (16, 17) — 20 µm." httpUri="https://zenodo.org/record/201655/files/figure.png" pageId="1" pageNumber="63">13</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="648266315F280315FF287B211BDFF8B7" httpUri="https://zenodo.org/record/201654/files/figure.png" pageId="2" pageNumber="64" targetBox="[168,1428,200,1590]" targetPageId="2">
|
||
<paragraph id="304236B95F280315FF287B211BDFF8B7" blockId="2.[151,1436,1634,1872]" pageId="2" pageNumber="64">
|
||
<emphasis id="0289EAAB5F280315FF287B211979F99F" bold="true" box="[151,314,1634,1657]" pageId="2" pageNumber="64">FIGURES 1–9.</emphasis>
|
||
<taxonomicName id="F7FD4D3A5F280315FEFF7B201A5AF99E" box="[320,537,1635,1657]" class="Gymnostomatea" family="Amphileptidae" genus="Amphileptus" kingdom="Protozoa" order="Pleurostomatida" pageId="2" pageNumber="64" phylum="Ciliophora" rank="species" species="salignus" status="sp. nov.">
|
||
<emphasis id="0289EAAB5F280315FEFF7B201A5AF99E" box="[320,537,1635,1657]" italics="true" pageId="2" pageNumber="64">Amphileptus salignus</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="19BA57D05F280315FDA07B201A1AF99F" box="[543,601,1635,1656]" pageId="2" pageNumber="64" rank="species">
|
||
<emphasis id="0289EAAB5F280315FDA07B201A1AF99F" bold="true" box="[543,601,1635,1656]" pageId="2" pageNumber="64">n. sp.</emphasis>
|
||
</taxonomicNameLabel>
|
||
from living cells (
|
||
<emphasis id="0289EAAB5F280315FCAB7B201B79F99F" bold="true" box="[788,826,1635,1657]" pageId="2" pageNumber="64">1–5</emphasis>
|
||
) and after protargol impregnation (
|
||
<emphasis id="0289EAAB5F280315FB1E7B201C84F99F" bold="true" box="[1185,1223,1635,1657]" pageId="2" pageNumber="64">6–9</emphasis>
|
||
). (
|
||
<emphasis id="0289EAAB5F280315FB5B7B201CB2F99F" bold="true" box="[1252,1265,1635,1656]" pageId="2" pageNumber="64">1</emphasis>
|
||
) Right view of a typical specimen with pointed posterior end. (
|
||
<emphasis id="0289EAAB5F280315FDC97BC11AC1F970" bold="true" box="[630,642,1666,1687]" pageId="2" pageNumber="64">2</emphasis>
|
||
) Two kinds of extrusomes. (
|
||
<emphasis id="0289EAAB5F280315FC0D7BC11BFDF970" bold="true" box="[946,958,1666,1687]" pageId="2" pageNumber="64">3</emphasis>
|
||
) Cortical granules distributed between ciliary rows. (
|
||
<emphasis id="0289EAAB5F280315FF627BE218AAF951" bold="true" box="[221,233,1697,1718]" pageId="2" pageNumber="64">4</emphasis>
|
||
) Left views, to show variations in body shape and in the number and distribution of contractile vacuoles. (
|
||
<emphasis id="0289EAAB5F280315FAA47BE21D64F951" bold="true" box="[1307,1319,1697,1718]" pageId="2" pageNumber="64">5</emphasis>
|
||
) Left view, note the dorsal brush. (
|
||
<emphasis id="0289EAAB5F280315FE3C7BFC19CCF933" bold="true" box="[387,399,1727,1748]" pageId="2" pageNumber="64">6</emphasis>
|
||
) Left-ventral view showing the detailed structure around the cytostome including the well-developed nematodesmata. (
|
||
<emphasis id="0289EAAB5F280315FEF47B9D1933F914" bold="true" box="[331,368,1758,1780]" pageId="2" pageNumber="64">7–8</emphasis>
|
||
) Infraciliature of right (7) and left (8) sides of the same specimen, arrows in Fig.
|
||
<emphasis id="0289EAAB5F280315FB037B9D1C8AF914" bold="true" box="[1212,1225,1758,1779]" pageId="2" pageNumber="64">7</emphasis>
|
||
mark the suture. (
|
||
<emphasis id="0289EAAB5F280315FA387B9D1DD7F914" bold="true" box="[1415,1428,1758,1779]" pageId="2" pageNumber="64">9</emphasis>
|
||
) Macronuclear nodules and distribution of the longer extrusomes, the shorter kind of extrusomes were not observed after protargol impregnation. CV: contractile vacuole, DB: dorsal brush, Ex: extrusome, Ma: macronuclear nodules, PK1–2: perioral kineties 1 and 2. Scale bars in (
|
||
<emphasis id="0289EAAB5F280315FE187A7919F7F8A8" bold="true" box="[423,436,1850,1871]" pageId="2" pageNumber="64">1</emphasis>
|
||
,
|
||
<emphasis id="0289EAAB5F280315FE7E7A79198DF8A8" bold="true" box="[449,462,1850,1871]" pageId="2" pageNumber="64">4</emphasis>
|
||
)—100 µm, in (
|
||
<emphasis id="0289EAAB5F280315FDD27A791A39F8A8" bold="true" box="[621,634,1850,1871]" pageId="2" pageNumber="64">2</emphasis>
|
||
) – 10 µm, in (7, 8)—80 µm.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="648266315F290314FF287DDB1C04FF2B" pageId="3" pageNumber="65">
|
||
<paragraph id="304236B95F290314FF287DDB1C04FF2B" blockId="3.[151,1436,152,625]" pageId="3" pageNumber="65">
|
||
<emphasis id="0289EAAB5F290314FF287DDB1944FF4A" bold="true" box="[151,263,152,173]" pageId="3" pageNumber="65">TABLE 1.</emphasis>
|
||
Morphometric data based on two populations of
|
||
<taxonomicName id="F7FD4D3A5F290314FCB47DDB1BA4FF49" box="[779,999,152,174]" class="Gymnostomatea" family="Amphileptidae" genus="Amphileptus" kingdom="Protozoa" order="Pleurostomatida" pageId="3" pageNumber="65" phylum="Ciliophora" rank="species" species="salignus" status="sp. nov.">
|
||
<emphasis id="0289EAAB5F290314FCB47DDB1BA4FF49" box="[779,999,152,174]" italics="true" pageId="3" pageNumber="65">Amphileptus salignus</emphasis>
|
||
</taxonomicName>
|
||
<taxonomicNameLabel id="19BA57D05F290314FC507DDB1C68FF4A" box="[1007,1067,152,173]" pageId="3" pageNumber="65" rank="species">
|
||
<emphasis id="0289EAAB5F290314FC507DDB1C68FF4A" bold="true" box="[1007,1067,152,173]" pageId="3" pageNumber="65">n. sp.</emphasis>
|
||
</taxonomicNameLabel>
|
||
(upper rows, Shenzhen population; lower rows, Hong Kong population). All data are based on protargol impregnated specimens.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="304236B95F290314FF207DAB1D02FC02" blockId="3.[151,1436,152,625]" lastBlockId="3.[159,1345,893,998]" pageId="3" pageNumber="65">
|
||
Characters Min Max Mean CV SD n Body length in µm 190 350 257.5 0.2 45.7 20 157 360 278.0 0.2 51.0 22 Body width in µm 43 78 56.9 0.2 9.9 20 32 56 47.7 0.1 5.9 22 Number of right somatic kineties a 24 29 27.4 0 1.2 19 24 29 28.0 19 1.7 20 Number of left somatic kineties b 4 4 4 0 0
|
||
<date id="444310795F290314FA977F491B79FDAF" pageId="3" pageNumber="65" value="1920-04-04">20 4 4</date>
|
||
4 0 0 21 Number of macronuclear nodules 2 2 2 0 0
|
||
<date id="444310795F290314FA977F1F1B79FC75" pageId="3" pageNumber="65" value="1920-01-01">20 1 1</date>
|
||
1 0 0 14 Length of micronucleus in µm 1.5 4 2.9 0.2 0.7
|
||
<date id="444310795F290314FA977EE41B7AFC01" pageId="3" pageNumber="65" value="1917-06-03">17 3 6</date>
|
||
4.6 0.2 1.0 14
|
||
</paragraph>
|
||
<paragraph id="304236B95F290314FD107FC61D02FC8E" pageId="3" pageNumber="65">
|
||
<table id="42FDC4195F29FCE8FF287FC61DDFFC8E" box="[151,1436,645,873]" gridcols="7" gridrows="4" pageId="3" pageNumber="65">
|
||
<tr id="8ECD34FB5F29FCE8FF287FC61DDFFD7D" box="[151,1436,645,666]" gridrow="0" pageId="3" pageNumber="65" rowspan-0="1">
|
||
<th id="CD1C5D875F29FCE8FD107FC61A8AFD7D" box="[687,713,645,666]" gridcol="1" gridrow="0" pageId="3" pageNumber="65">2</th>
|
||
<th id="CD1C5D875F29FCE8FC927FC61B05FD7D" box="[813,838,645,666]" gridcol="2" gridrow="0" pageId="3" pageNumber="65">2</th>
|
||
<th id="CD1C5D875F29FCE8FC137FC61B9BFD7D" box="[940,984,645,666]" gridcol="3" gridrow="0" pageId="3" pageNumber="65">2</th>
|
||
<th id="CD1C5D875F29FCE8FB957FC61C08FD7D" box="[1066,1099,645,666]" gridcol="4" gridrow="0" pageId="3" pageNumber="65">0</th>
|
||
<th id="CD1C5D875F29FCE8FB167FC61C8AFD7D" box="[1193,1225,645,666]" gridcol="5" gridrow="0" pageId="3" pageNumber="65">0</th>
|
||
<th id="CD1C5D875F29FCE8FA977FC61DDFFD7D" box="[1320,1436,645,666]" gridcol="6" gridrow="0" pageId="3" pageNumber="65">22</th>
|
||
</tr>
|
||
<tr id="8ECD34FB5F29FCE8FF287FED1DDFFD23" box="[151,1436,686,708]" gridrow="1" pageId="3" pageNumber="65">
|
||
<th id="CD1C5D875F29FCE8FF287FED1A6AFD23" box="[151,553,686,708]" gridcol="0" gridrow="1" pageId="3" pageNumber="65">Length of macronuclear nodules in µm</th>
|
||
<td id="CD1C5D875F29FCE8FD107FED1A8AFD23" box="[687,713,686,708]" gridcol="1" gridrow="1" pageId="3" pageNumber="65">30</td>
|
||
<td id="CD1C5D875F29FCE8FC927FED1B05FD23" box="[813,838,686,708]" gridcol="2" gridrow="1" pageId="3" pageNumber="65">60</td>
|
||
<td id="CD1C5D875F29FCE8FC137FED1B9BFD23" box="[940,984,686,708]" gridcol="3" gridrow="1" pageId="3" pageNumber="65">43.5</td>
|
||
<td id="CD1C5D875F29FCE8FB957FED1C08FD23" box="[1066,1099,686,708]" gridcol="4" gridrow="1" pageId="3" pageNumber="65">0.2</td>
|
||
<td id="CD1C5D875F29FCE8FB167FED1C8AFD23" box="[1193,1225,686,708]" gridcol="5" gridrow="1" pageId="3" pageNumber="65">9.0</td>
|
||
<td id="CD1C5D875F29FCE8FA977FED1DDFFD23" box="[1320,1436,686,708]" gridcol="6" gridrow="1" pageId="3" pageNumber="65">20</td>
|
||
</tr>
|
||
<tr id="8ECD34FB5F29FCE8FF287F9B1DDFFCF0" box="[151,1436,728,791]" gridrow="2" pageId="3" pageNumber="65">
|
||
<th id="CD1C5D875F29FCE8FF287F9B1A6AFCF0" box="[151,553,728,791]" gridcol="0" gridrow="2" pageId="3" pageNumber="65">Width of macronuclear nodules in µm</th>
|
||
<td id="CD1C5D875F29FCE8FD107F9B1A8AFCF0" box="[687,713,728,791]" gridcol="1" gridrow="2" pageId="3" pageNumber="65">28 18</td>
|
||
<td id="CD1C5D875F29FCE8FC927F9B1B05FCF0" box="[813,838,728,791]" gridcol="2" gridrow="2" pageId="3" pageNumber="65">55 36</td>
|
||
<td id="CD1C5D875F29FCE8FC137F9B1B9BFCF0" box="[940,984,728,791]" gridcol="3" gridrow="2" pageId="3" pageNumber="65">42.1 25.4</td>
|
||
<td id="CD1C5D875F29FCE8FB957F9B1C08FCF0" box="[1066,1099,728,791]" gridcol="4" gridrow="2" pageId="3" pageNumber="65">0.2 0.2</td>
|
||
<td id="CD1C5D875F29FCE8FB167F9B1C8AFCF0" box="[1193,1225,728,791]" gridcol="5" gridrow="2" pageId="3" pageNumber="65">8.2 4.2</td>
|
||
<td id="CD1C5D875F29FCE8FA977F9B1DDFFCF0" box="[1320,1436,728,791]" gridcol="6" gridrow="2" pageId="3" pageNumber="65">22 20</td>
|
||
</tr>
|
||
<tr id="8ECD34FB5F29FCE8FF287E681DDFFC8E" box="[151,1436,811,873]" gridrow="3" pageId="3" pageNumber="65">
|
||
<th id="CD1C5D875F29FCE8FF287E681A6AFC8E" box="[151,553,811,873]" gridcol="0" gridrow="3" pageId="3" pageNumber="65">Number of micronucleus</th>
|
||
<td id="CD1C5D875F29FCE8FD107E681A8AFC8E" box="[687,713,811,873]" gridcol="1" gridrow="3" pageId="3" pageNumber="65">11 1</td>
|
||
<td id="CD1C5D875F29FCE8FC927E681B05FC8E" box="[813,838,811,873]" gridcol="2" gridrow="3" pageId="3" pageNumber="65">19 1</td>
|
||
<td id="CD1C5D875F29FCE8FC137E681B9BFC8E" box="[940,984,811,873]" gridcol="3" gridrow="3" pageId="3" pageNumber="65">14.3 1</td>
|
||
<td id="CD1C5D875F29FCE8FB957E681C08FC8E" box="[1066,1099,811,873]" gridcol="4" gridrow="3" pageId="3" pageNumber="65">0.1 0</td>
|
||
<td id="CD1C5D875F29FCE8FB167E681C8AFC8E" box="[1193,1225,811,873]" gridcol="5" gridrow="3" pageId="3" pageNumber="65">1.8 0</td>
|
||
<td id="CD1C5D875F29FCE8FA977E681DDFFC8E" box="[1320,1436,811,873]" gridcol="6" gridrow="3" pageId="3" pageNumber="65">22 13</td>
|
||
</tr>
|
||
</table>
|
||
</paragraph>
|
||
<paragraph id="304236B95F290314FF2079611D02FB86" blockId="3.[159,1345,1058,1121]" pageId="3" pageNumber="65">
|
||
Length of extrusomes in µm 8 18 12.6 0.3 3.5
|
||
<date id="444310795F290314FA9779601B05FB86" pageId="3" pageNumber="65" value="1918-11-11">11 11 18</date>
|
||
14.7 0.2 2.2 20
|
||
</paragraph>
|
||
<paragraph id="304236B95F290314FF2879C11CEDFB5E" blockId="3.[151,1436,1154,1210]" pageId="3" pageNumber="65">a Includes perioral kinety 2; b Includes both perioral kinety 1 and dorsal brush kinety. Max, maximum; Mean, arithmetic mean; Min, minimum; n, number of cells measured; SD, standard deviation. CV, coefficient of variation in %.</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |