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<document id="7F8607B7B385863474FC0D21B37F96C9" ID-DOI="10.12651/JSR.2023.12.3.203" ID-ISSN="2713-8615" ID-Zenodo-Dep="13139732" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1722394104510" checkinUser="felipe" docAuthor="Yeo, Jeong Hyeon &amp; Jung, Pablo Quintela-Alonso and Jae-Ho" docDate="2023" docId="BC7687FC1700812C1437FB2DFC52FBF5" docLanguage="en" docName="JSpeciesRes.12.3.203-211.pdf" docOrigin="Journal of Species Research 12 (3)" docStyle="DocumentStyle:4D398BFF45FF8CA9E08F546BB06AE372.2:JSpeciesRes.2012-.journal_article" docStyleId="4D398BFF45FF8CA9E08F546BB06AE372" docStyleName="JSpeciesRes.2012-.journal_article" docStyleVersion="2" docTitle="Euplotes petzi Wilbert and Song 2008" docType="treatment" docVersion="2" lastPageNumber="207" masterDocId="404FFF8417038128171EFFE0FFA1FF86" masterDocTitle="New record of five Euplotes species (Protozoa, Ciliophora) collected from South Korea" masterLastPageNumber="211" masterPageNumber="203" pageNumber="206" updateTime="1722394507057" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="B6DF189BC568B21FB336A4F9FC2F6E89">New record of five Euplotes species (Protozoa, Ciliophora) collected from South Korea</mods:title>
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<mods:namePart id="6C4176A47CBBB1279A2ADEDE0C326FBE">Yeo, Jeong Hyeon</mods:namePart>
<mods:affiliation id="C2F81A985ED4B5AE71AB8F9286D4F611">Department of Biology, Gangneung-Wonju National University, Gangneung 25457, Republic of Korea</mods:affiliation>
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<mods:namePart id="1CBC6FBA7288A8C6904DBD8857BE2E28">Jung, Pablo Quintela-Alonso and Jae-Ho</mods:namePart>
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<mods:title id="2FD27985CC214F7A496D20A6129EC94B">Journal of Species Research</mods:title>
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<mods:date id="FAAAC6FCEAAA0E990F94A38ED7D035EC">2023</mods:date>
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<treatment id="BC7687FC1700812C1437FB2DFC52FBF5" ID-DOI="http://doi.org/10.5281/zenodo.13139748" ID-Zenodo-Dep="13139748" LSID="urn:lsid:plazi:treatment:BC7687FC1700812C1437FB2DFC52FBF5" httpUri="http://treatment.plazi.org/id/BC7687FC1700812C1437FB2DFC52FBF5" lastPageId="4" lastPageNumber="207" pageId="3" pageNumber="206">
<subSubSection id="7CC565611700812B1437FB2DFAE5FB63" box="[809,1348,1229,1253]" pageId="3" pageNumber="206" type="nomenclature">
<paragraph id="346036EA1700812B1437FB2DFAE5FB63" blockId="3.[809,1348,1229,1253]" box="[809,1348,1229,1253]" pageId="3" pageNumber="206">
<heading id="6F2881861700812B1437FB2DFAE5FB63" bold="true" box="[809,1348,1229,1253]" fontSize="36" level="2" pageId="3" pageNumber="206" reason="3">
<emphasis id="06ABEAF81700812B1437FB2DFAE5FB63" bold="true" box="[809,1348,1229,1253]" pageId="3" pageNumber="206">
4.
<taxonomicName id="F3DF4D691700812B145DFB2DFB4FFB63" ID-CoL="3CWBD" authority="Wilbert and Song, 2008" authorityName="Wilbert and Song" authorityYear="2008" box="[835,1262,1229,1253]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="206" phylum="Ciliophora" rank="species" species="petzi">
<emphasis id="06ABEAF81700812B145DFB2DFC7DFB63" bold="true" box="[835,988,1229,1253]" italics="true" pageId="3" pageNumber="206">Euplotes petzi</emphasis>
<bibRefCitation id="504E4B1B1700812B14FDFB2DFB4FFB63" author="Wilbert, N. &amp; W. Song" box="[995,1262,1229,1253]" pageId="3" pageNumber="206" pagination="979 - 1012" refId="ref6814" refString="Wilbert, N. and W. Song. 2008. A further study on littoral ciliates (Protozoa, Ciliophora) near King George Island, Antarctica, with description of a new genus and seven new species. Journal of Natural History 42: 979 - 1012." type="journal article" year="2008">Wilbert and Song, 2008</bibRefCitation>
</taxonomicName>
(
<figureCitation id="ACE42A6F1700812B13E5FB2DFA9DFB63" box="[1275,1340,1229,1253]" captionStart="Fig" captionStartId="4.[174,207,943,965]" captionTargetBox="[190,1441,262,873]" captionTargetId="figure-467@4.[174,1444,259,910]" captionTargetPageId="4" captionText="Fig. 4. Euplotes petzi after protargol(A, B) and after “wet” silver nitrate impregnation (C). A, B. Ventral (A) and dorsal (B) view of a representative specimen. Note the narrow separation between the two marginal cirri, a distinctive characteristic of this species. C. Dorsal view showing the argyrome in double-patella pattern. AZM, adoral zone of membranelles; CC; caudal cirri; DK, dorsal kineties; FVC, frontoventral cirri; MC, marginal cirri. Scale bars: 30μm." figureDoi="http://doi.org/10.5281/zenodo.13139740" httpUri="https://zenodo.org/record/13139740/files/figure.png" pageId="3" pageNumber="206">Fig. 4</figureCitation>
)
</emphasis>
</heading>
</paragraph>
</subSubSection>
<subSubSection id="7CC565611700812C1437FAEDFBB4FC0F" lastPageId="4" lastPageNumber="207" pageId="3" pageNumber="206" type="materials_examined">
<paragraph id="346036EA1700812B1437FAEDFC61FA00" blockId="3.[809,1414,1293,1415]" pageId="3" pageNumber="206">
<emphasis id="06ABEAF81700812B1437FAEDFBA9FAA3" bold="true" box="[809,1032,1293,1317]" pageId="3" pageNumber="206">Material examined.</emphasis>
<materialsCitation id="84B73CB71700812B1310FAEDFC1CFA00" collectingDate="2021-01-21" country="South Korea" county="Seocheon-gun" latitude="36.015278" location="Marine" longLatPrecision="19" longitude="126.66361" municipality="Janghang-eup" pageId="3" pageNumber="206" specimenCount="1" stateProvince="Chungnam">
<location id="310060311700812B1310FAEDFBFBFAA3" LSID="urn:lsid:plazi:treatment:BC7687FC1700812C1437FB2DFC52FBF5:310060311700812B1310FAEDFBFBFAA3" box="[1038,1114,1293,1317]" country="South Korea" county="Seocheon-gun" latitude="36.015278" longLatPrecision="19" longitude="126.66361" municipality="Janghang-eup" name="Marine" pageId="3" pageNumber="206" stateProvince="Chungnam">Marine</location>
water (salinity 28‰) collected from
<location id="310060311700812B1497FACEFC4AFAC0" LSID="urn:lsid:plazi:treatment:BC7687FC1700812C1437FB2DFC52FBF5:310060311700812B1497FACEFC4AFAC0" box="[905,1003,1326,1350]" country="South Korea" county="Seocheon-gun" latitude="36.015278" longLatPrecision="19" longitude="126.66361" municipality="Janghang-eup" name="West Sea" pageId="3" pageNumber="206" stateProvince="Chungnam">West Sea</location>
,
<collectingMunicipality id="D404AC901700812B14E8FACEFB2BFAC0" box="[1014,1162,1326,1350]" pageId="3" pageNumber="206">Janghang-eup</collectingMunicipality>
,
<collectingCounty id="DD014E661700812B138AFACEFA8DFAC0" box="[1172,1324,1326,1350]" pageId="3" pageNumber="206">Seocheon-gun</collectingCounty>
,
<collectingRegion id="F61BF8081700812B1229FACEFC66FAE0" country="South Korea" name="Chungnam" pageId="3" pageNumber="206">Chungcheongnam-do</collectingRegion>
,
<collectingCountry id="4CC8767A1700812B14CEFAAFFBB1FAE0" box="[976,1040,1359,1382]" name="South Korea" pageId="3" pageNumber="206">Korea</collectingCountry>
(
<geoCoordinate id="51EB502D1700812B1305FAAEFB3BFAE0" box="[1051,1178,1357,1382]" degrees="36" direction="north" minutes="00" orientation="latitude" pageId="3" pageNumber="206" precision="15" seconds="55" value="36.015278">36°0055″N</geoCoordinate>
,
<geoCoordinate id="51EB502D1700812B13BBFAAEFA8DFAE0" box="[1189,1324,1357,1382]" degrees="126" direction="east" minutes="39" orientation="longitude" pageId="3" pageNumber="206" precision="15" seconds="49" value="126.66361">126°3949″E</geoCoordinate>
) on
<date id="4061102A1700812B1244FAAFFC1CFA00" pageId="3" pageNumber="206" value="2021-01-21">
<collectingDate id="5025E9C21700812B1244FAAFFC1CFA00" pageId="3" pageNumber="206" value="2021-01-21">January 21, 2021</collectingDate>
</date>
</materialsCitation>
.
</paragraph>
<caption id="60A066621700812B178EF88BFADBF830" ID-DOI="http://doi.org/10.5281/zenodo.13139738" ID-Zenodo-Dep="13139738" httpUri="https://zenodo.org/record/13139738/files/figure.png" pageId="3" pageNumber="206" startId="3.[144,177,1899,1921]" targetBox="[147,1414,1480,1866]" targetPageId="3" targetType="figure">
<paragraph id="346036EA1700812B178EF88BFADBF830" blockId="3.[144,1415,1899,1975]" pageId="3" pageNumber="206">
<emphasis id="06ABEAF81700812B178EF88BFF6CF806" bold="true" box="[144,205,1899,1921]" pageId="3" pageNumber="206">Fig. 3.</emphasis>
<taxonomicName id="F3DF4D691700812B17CDF88BFE07F807" authorityName="Carter" authorityYear="1972" box="[211,422,1899,1921]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="206" phylum="Ciliophora" rank="species" species="octocarinatus">
<emphasis id="06ABEAF81700812B17CDF88BFE07F807" box="[211,422,1899,1921]" italics="true" pageId="3" pageNumber="206">Euplotes octocarinatus</emphasis>
</taxonomicName>
in vivo (A, B) and after protargol (C, D) and “wet” silver nitrate impregnation (E). A- D. Ventral (A, C) and dorsal (B, D) views of different specimens showing the infraciliature and nuclear apparatus. E. Dorsal argyrome of double-
<taxonomicName id="F3DF4D691700812B120FF866FAF1F81D" authorityName="Ehrenberg" authorityYear="1838" baseAuthorityName="Muller" baseAuthorityYear="1773" box="[1297,1360,1926,1947]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="206" phylum="Ciliophora" rank="species" species="patella">
<emphasis id="06ABEAF81700812B120FF866FAF1F81D" box="[1297,1360,1926,1947]" italics="true" pageId="3" pageNumber="206">patella</emphasis>
</taxonomicName>
type. AZM, adoral zone of membranelles; CC, caudal cirri; DK, dorsal kineties; FVC, frontoventral cirri; MC, marginal cirri. Scale bars: 50 μm.
</paragraph>
</caption>
<paragraph id="346036EA1707812C17A4FC8BFBB4FC0F" blockId="4.[186,669,875,905]" box="[186,1045,875,905]" lastBlockId="4.[1021,1045,875,905]" pageId="4" pageNumber="207">
<emphasis id="06ABEAF81707812C17A4FC8BFBB4FC0F" bold="true" box="[186,1045,875,905]" pageId="4" pageNumber="207">A B C</emphasis>
</paragraph>
</subSubSection>
<caption id="60A066621707812C17B0FC4FFD32FB92" ID-DOI="http://doi.org/10.5281/zenodo.13139740" ID-Zenodo-Dep="13139740" httpUri="https://zenodo.org/record/13139740/files/figure.png" pageId="4" pageNumber="207" startId="4.[174,207,943,965]" targetBox="[190,1441,262,873]" targetPageId="4" targetType="figure">
<paragraph id="346036EA1707812C17B0FC4FFD32FB92" blockId="4.[174,1444,943,1045]" pageId="4" pageNumber="207">
<emphasis id="06ABEAF81707812C17B0FC4FFF4BFC42" bold="true" box="[174,234,943,965]" pageId="4" pageNumber="207">Fig. 4.</emphasis>
<taxonomicName id="F3DF4D691707812C17EEFC4FFED1FC42" authorityName="Wilbert and Song" authorityYear="2008" box="[240,368,943,964]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="207" phylum="Ciliophora" rank="species" species="petzi">
<emphasis id="06ABEAF81707812C17EEFC4FFED1FC42" box="[240,368,943,964]" italics="true" pageId="4" pageNumber="207">Euplotes petzi</emphasis>
</taxonomicName>
after protargol (A, B) and after “wet” silver nitrate impregnation (C). A, B. Ventral (A) and dorsal (B) view of a representative specimen. Note the narrow separation between the two marginal cirri, a distinctive characteristic of this species. C. Dorsal view showing the argyrome in double-
<taxonomicName id="F3DF4D691707812C16FBFC04FD84FC7F" authorityName="Ehrenberg" authorityYear="1838" baseAuthorityName="Muller" baseAuthorityYear="1773" box="[485,549,996,1017]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="207" phylum="Ciliophora" rank="species" species="patella">
<emphasis id="06ABEAF81707812C16FBFC04FD84FC7F" box="[485,549,996,1017]" italics="true" pageId="4" pageNumber="207">patella</emphasis>
</taxonomicName>
pattern. AZM, adoral zone of membranelles; CC; caudal cirri; DK, dorsal kineties; FVC, frontoventral cirri; MC, marginal cirri. Scale bars: 30 μm.
</paragraph>
</caption>
<subSubSection id="7CC565611707812C17B0FBBBFE6BFAD3" pageId="4" pageNumber="207" type="diagnosis">
<paragraph id="346036EA1707812C17B0FBBBFE6BFAD3" blockId="4.[174,779,1115,1978]" pageId="4" pageNumber="207">
<emphasis id="06ABEAF81707812C17B0FBBBFE83FBF5" bold="true" box="[174,290,1115,1139]" pageId="4" pageNumber="207">Diagnosis.</emphasis>
Body size 38.2-46.2 × 25.3-36.7 μm (on average 42.6 × 29.9 μm) after protargol impregnation, shape ellipsoidal; macronucleus hook-shaped, with one spherical micronucleus attached; 35-45 adoral membranelles; 10 frontoventral, 5 transverse, 2 caudal and 2 marginal cirri; invariably 6 dorsal kineties, of which the middle kinety composed of about 8-10 dikinetids; dorsal argyrome pattern in double-
<taxonomicName id="F3DF4D691707812C1659FADDFE2EFAD3" authorityName="Ehrenberg" authorityYear="1838" baseAuthorityName="Muller" baseAuthorityYear="1773" box="[327,399,1341,1365]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="207" phylum="Ciliophora" rank="species" species="patella">
<emphasis id="06ABEAF81707812C1659FADDFE2EFAD3" box="[327,399,1341,1365]" italics="true" pageId="4" pageNumber="207">patella</emphasis>
</taxonomicName>
<typeStatus id="EB6488481707812C168BFADDFE64FAD3" box="[405,453,1341,1365]" pageId="4" pageNumber="207">type</typeStatus>
.
</paragraph>
</subSubSection>
<subSubSection id="7CC565611707812C17B0FABDFDA5FA13" pageId="4" pageNumber="207" type="distribution">
<paragraph id="346036EA1707812C17B0FABDFDA5FA13" blockId="4.[174,779,1115,1978]" pageId="4" pageNumber="207">
<emphasis id="06ABEAF81707812C17B0FABDFEE9FAF3" bold="true" box="[174,328,1373,1397]" pageId="4" pageNumber="207">Distribution.</emphasis>
King George Island (
<bibRefCitation id="504E4B1B1707812C1522FABDFF45FA10" author="Wilbert, N. &amp; W. Song" pageId="4" pageNumber="207" pagination="979 - 1012" refId="ref6814" refString="Wilbert, N. and W. Song. 2008. A further study on littoral ciliates (Protozoa, Ciliophora) near King George Island, Antarctica, with description of a new genus and seven new species. Journal of Natural History 42: 979 - 1012." type="journal article" year="2008">Wilbert and Song, 2008</bibRefCitation>
) and
<collectingCountry id="4CC8767A1707812C1603FA9EFEFCFA13" box="[285,349,1406,1429]" name="South Korea" pageId="4" pageNumber="207">Korea</collectingCountry>
(present study).
</paragraph>
</subSubSection>
<subSubSection id="7CC565611707812C17B0FA7EFC52FBF5" pageId="4" pageNumber="207" type="discussion">
<paragraph id="346036EA1707812C17B0FA7EFDDEF8BF" blockId="4.[174,779,1115,1978]" pageId="4" pageNumber="207">
<emphasis id="06ABEAF81707812C17B0FA7EFE82FA30" bold="true" box="[174,291,1438,1462]" pageId="4" pageNumber="207">Remarks.</emphasis>
The morphology of the Korean and King George Island populations of
<taxonomicName id="F3DF4D691707812C16F5FA5EFE93FA70" authority="(Wilbert and Song, 2008)" baseAuthorityName="Wilbert and Song" baseAuthorityYear="2008" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="207" phylum="Ciliophora" rank="species" species="petzi">
<emphasis id="06ABEAF81707812C16F5FA5EFDDEFA50" box="[491,639,1470,1494]" italics="true" pageId="4" pageNumber="207">Euplotes petzi</emphasis>
(
<bibRefCitation id="504E4B1B1707812C1592FA5EFE8AFA70" author="Wilbert, N. &amp; W. Song" pageId="4" pageNumber="207" pagination="979 - 1012" refId="ref6814" refString="Wilbert, N. and W. Song. 2008. A further study on littoral ciliates (Protozoa, Ciliophora) near King George Island, Antarctica, with description of a new genus and seven new species. Journal of Natural History 42: 979 - 1012." type="journal article" year="2008">Wilbert and Song, 2008</bibRefCitation>
)
</taxonomicName>
is mostly similar except cell size (38-46 × 25-37 μm, and 50-80 × 30-50 μm). A distinctive morphological feature of this species is the narrow separation between the two marginal cirri compared to other
<taxonomicName id="F3DF4D691707812C15AEF9DFFEA0F9F1" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="207" phylum="Ciliophora" rank="species" species="undetermined">
<emphasis id="06ABEAF81707812C15AEF9DFFCAAF9D1" box="[688,779,1599,1623]" italics="true" pageId="4" pageNumber="207">Euplotes</emphasis>
species.
</taxonomicName>
<bibRefCitation id="504E4B1B1707812C1619F9BFFDB5F9F1" author="Di Giuseppe, G. &amp; F. Erra &amp; F. P. Frontini &amp; F. Dini &amp; A. Vallesi &amp; P. Luporini" box="[263,532,1631,1655]" pageId="4" pageNumber="207" pagination="402 - 411" refId="ref5088" refString="Di Giuseppe, G., F. Erra, F. P. Frontini, F. Dini, A. Vallesi and P. Luporini. 2014. Improved description of the bipolar ciliate, Euplotes petzi, and definition of its basal position in the Euplotes phylogenetic tree. European Journal of Protistology 50: 402 - 411." type="journal article" year="2014">
Di Giuseppe
<emphasis id="06ABEAF81707812C168AF980FE6BF9F1" box="[404,458,1631,1655]" italics="true" pageId="4" pageNumber="207">et al.</emphasis>
(2014)
</bibRefCitation>
improved the morphological characterization of
<taxonomicName id="F3DF4D691707812C16D0F960FD81F911" baseAuthorityName="Wilbert and Song" baseAuthorityYear="2008" box="[462,544,1664,1687]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="207" phylum="Ciliophora" rank="species" species="petzi">
<emphasis id="06ABEAF81707812C16D0F960FD81F911" box="[462,544,1664,1687]" italics="true" pageId="4" pageNumber="207">E. petzi</emphasis>
</taxonomicName>
and used SSU rDNA gene sequences to relate this species to
<taxonomicName id="F3DF4D691707812C157EF940FE95F95E" authority="Jiang et al., 2010 b" authorityName="Jiang" authorityYear="2010" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="207" phylum="Ciliophora" rank="species" species="sinicus">
<emphasis id="06ABEAF81707812C157EF940FD6BF931" box="[608,714,1696,1719]" italics="true" pageId="4" pageNumber="207">E. sinicus</emphasis>
<bibRefCitation id="504E4B1B1707812C15CDF940FE95F95E" author="Jiang, J. &amp; Q. Zhang &amp; X. Hu &amp; C. Shao &amp; K. A. S. Al-Rasheid &amp; W. Song" pageId="4" pageNumber="207" pagination="1241 - 1251" refId="ref5506" refString="Jiang, J., Q. Zhang, X. Hu, C. Shao, K. A. S. Al-Rasheid and W. Song. 2010 b. Two new marine ciliates, Euplotes sinicus sp. nov. and Euplotes parabalteatus sp. nov., and a new small subunit rRNA gene sequence of Euplotes rariseta (Ciliophora, Spirotrichea, Euplotida). International Journal of Systematic and Evolutionary Microbiology 60: 1241 - 1251." type="journal article" year="2010">
Jiang
<emphasis id="06ABEAF81707812C17B0F921FF45F95E" box="[174,228,1728,1752]" italics="true" pageId="4" pageNumber="207">et al.</emphasis>
, 2010b
</bibRefCitation>
</taxonomicName>
as the deepest branch at the base of the
<taxonomicName id="F3DF4D691707812C15FBF921FF4EF97E" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="207" phylum="Ciliophora" rank="genus">
<emphasis id="06ABEAF81707812C15FBF921FF4EF97E" italics="true" pageId="4" pageNumber="207">Euplotes</emphasis>
</taxonomicName>
phylogenetic tree. Moreover, their investigation revealed notable similarities between these two species in terms of the cell size and key characteristics.
</paragraph>
<paragraph id="346036EA1707812C17B0F8A1FC52FBF5" blockId="4.[174,779,1115,1978]" lastBlockId="4.[839,1011,1115,1139]" pageId="4" pageNumber="207">
<emphasis id="06ABEAF81707812C17B0F8A1FEC0F8DF" bold="true" box="[174,353,1857,1881]" pageId="4" pageNumber="207">Voucher slides.</emphasis>
One slide with protargol-impregnated specimens (NNIBRPR25661) and one slide with wet silver nitrate-impregnated specimens (NNIBRPR25662) were deposited at the Nakdonggang National Institute of Biological Resources.
</paragraph>
</subSubSection>
</treatment>
</document>