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<document id="8AD28334815715F9B444E909A5BACF02" ID-DOI="10.5281/zenodo.174287" ID-GBIF-Dataset="26ac73b9-1c53-4f82-bc02-78a055ba31e9" ID-ISSN="1175-5326" ID-Zenodo-Dep="174287" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1459794690970" checkinUser="plazi" docAuthor="Hooge, Matthew D. &amp; Rocha, Carlos E. F." docDate="2006" docId="03DE87D4E605FF90FE84117FFEC9FB2B" docLanguage="en" docName="zt01335p050.pdf" docOrigin="Zootaxa 1335" docStyle="DocumentStyle:FA7E419B012A62B0FC3AC15A186C3DAF.3:Zootaxa.2001-2006.journal_article" docStyleId="FA7E419B012A62B0FC3AC15A186C3DAF" docStyleName="Zootaxa.2001-2006.journal_article" docStyleVersion="3" docTitle="Philocelis robrochai Hooge &amp; Rocha, 2006, sp. nov." docType="treatment" docVersion="9" lastPageNumber="45" masterDocId="FFE7FFACE62DFFBCFF8C140EFF97FFE5" masterDocTitle="Acoela (Acoelomorpha) from the northern beaches of the state of São Paulo, Brazil, and a systematic revision of the family Otocelididae" masterLastPageNumber="50" masterPageNumber="1" pageNumber="41" updateTime="1698219545359" updateUser="plazi">
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<mods:title id="F1926EA4CA27E4C9ADAAE7C551A4DF7D">Acoela (Acoelomorpha) from the northern beaches of the state of São Paulo, Brazil, and a systematic revision of the family Otocelididae</mods:title>
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<mods:namePart id="1A196AA2F68C3AA13CA7BE9118C4FA71">Hooge, Matthew D.</mods:namePart>
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<mods:namePart id="0583EE36D6D30F64A63D226D5B29562D">Rocha, Carlos E. F.</mods:namePart>
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<mods:date id="73F7AA1DFC0E53081D75D24DFE818EBB">2006</mods:date>
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<treatment id="03DE87D4E605FF90FE84117FFEC9FB2B" ID-DOI="http://doi.org/10.5281/zenodo.6263461" ID-GBIF-Taxon="119350917" ID-Zenodo-Dep="6263461" LSID="urn:lsid:plazi:treatment:03DE87D4E605FF90FE84117FFEC9FB2B" httpUri="http://treatment.plazi.org/id/03DE87D4E605FF90FE84117FFEC9FB2B" lastPageId="44" lastPageNumber="45" pageId="40" pageNumber="41">
<subSubSection id="C36D6549E605FF94FE84117FFE31FA4B" pageId="40" pageNumber="41" type="nomenclature">
<paragraph id="8BC836C2E605FF94FE84117FFDF5FA6E" blockId="40.[264,610,1393,1454]" box="[264,610,1393,1419]" pageId="40" pageNumber="41">
<heading id="D08081AEE605FF94FE84117FFDF5FA6E" bold="true" box="[264,610,1393,1419]" fontSize="11" level="1" pageId="40" pageNumber="41" reason="1">
<emphasis id="B903EAD0E605FF94FE84117FFDF5FA6E" bold="true" box="[264,610,1393,1419]" pageId="40" pageNumber="41">
<taxonomicName id="4C774D41E605FF94FE84117FFE6AFA6E" box="[264,509,1393,1419]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="40" pageNumber="41" phylum="Xenacoelomorpha" rank="species" species="robrochai" status="sp. nov.">
<emphasis id="B903EAD0E605FF94FE84117FFE6AFA6E" bold="true" box="[264,509,1393,1419]" italics="true" pageId="40" pageNumber="41">Philocelis robrochai</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A23057ABE605FF94FD88117FFDF5FA6E" box="[516,610,1393,1419]" pageId="40" pageNumber="41" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
</heading>
</paragraph>
<paragraph id="8BC836C2E605FF94FE84119AFE31FA4B" blockId="40.[264,610,1393,1454]" box="[264,422,1428,1454]" pageId="40" pageNumber="41">
(
<figureCitation id="134C2A47E605FF94FE9D119AFE09FA4B" box="[273,414,1428,1454]" captionStart-0="FIGURE 27" captionStart-1="FIGURE 28" captionStart-2="FIGURE 29" captionStartId-0="41.[264,367,1796,1820]" captionStartId-1="42.[264,367,1495,1519]" captionStartId-2="43.[264,367,1333,1357]" captionTargetBox-0="[315,1278,326,1774]" captionTargetBox-1="[273,1314,327,1462]" captionTargetBox-2="[264,1322,406,1309]" captionTargetId-0="figure@41.[315,1278,326,1775]" captionTargetId-1="figure@42.[273,1315,326,1471]" captionTargetId-2="figure@43.[264,1323,406,1309]" captionTargetPageId-0="41" captionTargetPageId-1="42" captionTargetPageId-2="43" captionText-0="FIGURE 27. Philocelis robrochai sp. nov.; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs." captionText-1="FIGURE 28. Philocelis robrochai sp. nov.; reconstructions to show arrangement of organs. A. Sagittal reconstruction showing arrangement of female copulatory organ. B. Sagittal reconstruction showing arrangement of male copulatory organ." captionText-2="FIGURE 29. Philocelis robrochai sp. nov.; whole-mounts stained with Alexa- 488 - labeled phalloidin and viewed with confocal microscopy. A. Ventral projection of posterior end of animal. B. Projection of male copulatory organ." httpUri-0="https://zenodo.org/record/174313/files/figure.png" httpUri-1="https://zenodo.org/record/174314/files/figure.png" httpUri-2="https://zenodo.org/record/174315/files/figure.png" pageId="40" pageNumber="41">Figs. 2729</figureCitation>
)
</paragraph>
</subSubSection>
<subSubSection id="C36D6549E605FF94FE8411EAFBD1F883" pageId="40" pageNumber="41" type="materials_examined">
<paragraph id="8BC836C2E605FF94FE8411EAFED5F993" blockId="40.[264,1322,1508,1894]" pageId="40" pageNumber="41">
<emphasis id="B903EAD0E605FF94FE8411EAFE2FFA1B" bold="true" box="[264,440,1508,1534]" pageId="40" pageNumber="41">
<typeStatus id="54CC8860E605FF94FE8411EAFED4FA1B" box="[264,323,1508,1534]" pageId="40" pageNumber="41">Type</typeStatus>
Material
</emphasis>
.
<typeStatus id="54CC8860E605FF94FE4711EAFDABFA1B" box="[459,572,1508,1534]" pageId="40" pageNumber="41" type="holotype">Holotype</typeStatus>
.
<collectionCode id="ED66AE07E605FF94FDCB11EAFD3EFA1B" box="[583,681,1508,1534]" httpUri="http://grbio.org/cool/6yx0-3nm7" name="Museu de Zoologia da Universidade de Sao Paulo" pageId="40" pageNumber="41">MZUSP</collectionCode>
<collectionCode id="ED66AE07E605FF94FD3D11EAFD40FA1B" box="[689,727,1508,1534]" pageId="40" pageNumber="41">PL</collectionCode>
. 196, one set of 2-µm-thick serial sagittal sections of epoxy-embedded specimen stained with toluidine blue.
<typeStatus id="54CC8860E605FF94FC391202FB88F9C3" box="[949,1055,1548,1574]" pageId="40" pageNumber="41" type="paratype">Paratype</typeStatus>
.
<collectionCode id="ED66AE07E605FF94FBA71202FB1AF9C3" box="[1067,1165,1548,1574]" httpUri="http://grbio.org/cool/6yx0-3nm7" name="Museu de Zoologia da Universidade de Sao Paulo" pageId="40" pageNumber="41">MZUSP</collectionCode>
<collectionCode id="ED66AE07E605FF94FB181202FB2DF9C3" box="[1172,1210,1548,1574]" pageId="40" pageNumber="41">PL</collectionCode>
. 197, one set of 2-µm-thick oblique sections of epoxy-embedded specimen stained with toluidine blue.
</paragraph>
<paragraph id="8BC836C2E605FF94FEB4128AFD2CF923" blockId="40.[264,1322,1508,1894]" pageId="40" pageNumber="41">
<emphasis id="B903EAD0E605FF94FEB4128AFE42F97B" bold="true" box="[312,469,1668,1694]" pageId="40" pageNumber="41">
<typeStatus id="54CC8860E605FF94FEB4128AFEE2F97B" box="[312,373,1668,1694]" pageId="40" pageNumber="41">Type</typeStatus>
locality
</emphasis>
. Praia da Vila, Ilhabela, São Paulo,
<collectingCountry id="F3607652E605FF94FC0F128AFC59F97B" box="[899,974,1668,1694]" name="Brazil" pageId="40" pageNumber="41">Brazil</collectingCountry>
, from subtidal coarse-grained sand (
<geoCoordinate id="EE435005E605FF94FEC212A2FE66F923" box="[334,497,1708,1734]" direction="south" orientation="latitude" pageId="40" pageNumber="41" precision="1" value="-23.779055">23°4644.6”S</geoCoordinate>
,
<geoCoordinate id="EE435005E605FF94FE7212A2FD38F923" box="[510,687,1708,1734]" direction="west" orientation="longitude" pageId="40" pageNumber="41" precision="1" value="-45.359055">45°2132.6”W</geoCoordinate>
).
</paragraph>
<paragraph id="8BC836C2E605FF94FEB412DAFBD1F883" blockId="40.[264,1322,1508,1894]" pageId="40" pageNumber="41">
<emphasis id="B903EAD0E605FF94FEB412DAFDEFF90B" bold="true" box="[312,632,1748,1774]" pageId="40" pageNumber="41">Other material examined</emphasis>
. Living specimens in squeeze preparations from Praia de Pequeá, Ilhabela; three sets of 2-µm-thick serial oblique sections of epoxy-embedded specimens stained with toluidine blue; whole mounts for fluorescence imaging of musculature; photographs of living specimens in squeeze preparations.
</paragraph>
</subSubSection>
<caption id="DF08664AE604FF95FE84130AFC9CF884" httpUri="https://zenodo.org/record/174313/files/figure.png" pageId="41" pageNumber="42" targetBox="[315,1278,326,1774]" targetPageId="41">
<paragraph id="8BC836C2E604FF95FE84130AFC9CF884" blockId="41.[264,1323,1796,1889]" pageId="41" pageNumber="42">
<emphasis id="B903EAD0E604FF95FE84130AFE00F8F9" bold="true" box="[264,407,1796,1820]" pageId="41" pageNumber="42">FIGURE 27.</emphasis>
<taxonomicName id="4C774D41E604FF95FE12130BFDEEF8F9" box="[414,633,1797,1820]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="41" pageNumber="42" phylum="Xenacoelomorpha" rank="species" species="robrochai" status="sp. nov.">
<emphasis id="B903EAD0E604FF95FE12130BFDEEF8F9" box="[414,633,1797,1820]" italics="true" pageId="41" pageNumber="42">Philocelis robrochai</emphasis>
</taxonomicName>
<emphasis id="B903EAD0E604FF95FDF3130AFD43F8F9" bold="true" box="[639,724,1796,1820]" pageId="41" pageNumber="42">
<taxonomicNameLabel id="A23057ABE604FF95FDF3130AFD43F8F9" box="[639,724,1796,1820]" pageId="41" pageNumber="42" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs.
</paragraph>
</caption>
<caption id="DF08664AE607FF96FE8411D9FC9FF9D1" httpUri="https://zenodo.org/record/174314/files/figure.png" pageId="42" pageNumber="43" targetBox="[273,1314,327,1462]" targetPageId="42">
<paragraph id="8BC836C2E607FF96FE8411D9FC9FF9D1" blockId="42.[264,1323,1495,1588]" pageId="42" pageNumber="43">
<emphasis id="B903EAD0E607FF96FE8411D9FE0CFA0A" bold="true" box="[264,411,1495,1519]" pageId="42" pageNumber="43">FIGURE 28.</emphasis>
<taxonomicName id="4C774D41E607FF96FE2A11D6FD13FA0A" box="[422,644,1496,1519]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="42" pageNumber="43" phylum="Xenacoelomorpha" rank="species" species="robrochai" status="sp. nov.">
<emphasis id="B903EAD0E607FF96FE2A11D6FD13FA0A" box="[422,644,1496,1519]" italics="true" pageId="42" pageNumber="43">Philocelis robrochai</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A23057ABE607FF96FD0211D9FD7FFA0A" box="[654,744,1495,1519]" pageId="42" pageNumber="43" rank="species">
<emphasis id="B903EAD0E607FF96FD0211D9FD7FFA0A" bold="true" box="[654,744,1495,1519]" pageId="42" pageNumber="43">sp. nov.</emphasis>
</taxonomicNameLabel>
; reconstructions to show arrangement of organs. A. Sagittal reconstruction showing arrangement of female copulatory organ. B. Sagittal reconstruction showing arrangement of male copulatory organ.
</paragraph>
</caption>
<subSubSection id="C36D6549E607FF96FEB4126CFB3DF999" box="[312,1194,1634,1660]" pageId="42" pageNumber="43" type="etymology">
<paragraph id="8BC836C2E607FF96FEB4126CFB3DF999" blockId="42.[264,1323,1634,1900]" box="[312,1194,1634,1660]" pageId="42" pageNumber="43">
<emphasis id="B903EAD0E607FF96FEB4126CFE2AF999" bold="true" box="[312,445,1634,1660]" pageId="42" pageNumber="43">Etymology</emphasis>
. Species name in honor of Mr. Roberto Rocha of San Jose, CA.
</paragraph>
</subSubSection>
<subSubSection id="C36D6549E607FF90FEB41284FCC9FDAB" lastPageId="44" lastPageNumber="45" pageId="42" pageNumber="43" type="description">
<paragraph id="8BC836C2E607FF97FEB41284FD92FEBB" blockId="42.[264,1323,1634,1900]" lastBlockId="43.[264,1322,284,350]" lastPageId="43" lastPageNumber="44" pageId="42" pageNumber="43">
<emphasis id="B903EAD0E607FF96FEB41284FE5EF941" bold="true" box="[312,457,1674,1700]" pageId="42" pageNumber="43">Description</emphasis>
. Living specimens ~500 µm long and ~140 µm wide (
<figureCitation id="134C2A47E607FF96FBF51284FB49F941" box="[1145,1246,1674,1700]" captionStart="FIGURE 27" captionStartId="41.[264,367,1796,1820]" captionTargetBox="[315,1278,326,1774]" captionTargetId="figure@41.[315,1278,326,1775]" captionTargetPageId="41" captionText="FIGURE 27. Philocelis robrochai sp. nov.; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs." httpUri="https://zenodo.org/record/174313/files/figure.png" pageId="42" pageNumber="43">Figs. 27</figureCitation>
A, B). Anterior and posterior ends rounded. Body cylindrical. Epidermis completely ciliated. Rhabdoid glands in distinct rows (
<figureCitation id="134C2A47E607FF96FD2D12D4FD6FF911" box="[673,760,1754,1780]" captionStart="FIGURE 27" captionStartId="41.[264,367,1796,1820]" captionTargetBox="[315,1278,326,1774]" captionTargetId="figure@41.[315,1278,326,1775]" captionTargetPageId="41" captionText="FIGURE 27. Philocelis robrochai sp. nov.; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs." httpUri="https://zenodo.org/record/174313/files/figure.png" pageId="42" pageNumber="43">Fig. 27</figureCitation>
A, C). Mucoid glands present mostly in ventral body wall (
<figureCitation id="134C2A47E607FF96FE19130CFE78F8F9" box="[405,495,1794,1820]" captionStart="FIGURE 28" captionStartId="42.[264,367,1495,1519]" captionTargetBox="[273,1314,327,1462]" captionTargetId="figure@42.[273,1315,326,1471]" captionTargetPageId="42" captionText="FIGURE 28. Philocelis robrochai sp. nov.; reconstructions to show arrangement of organs. A. Sagittal reconstruction showing arrangement of female copulatory organ. B. Sagittal reconstruction showing arrangement of male copulatory organ." httpUri="https://zenodo.org/record/174314/files/figure.png" pageId="42" pageNumber="43">Fig. 28</figureCitation>
A). Body colorless in transmitted light, but digestive syncytium has orange coloration. Frontal organ well developed. Cell bodies of frontal glands positioned 70 µm behind frontal pore (
<figureCitation id="134C2A47E607FF96FDC2135CFD27F889" box="[590,688,1874,1900]" captionStart="FIGURE 27" captionStartId="41.[264,367,1796,1820]" captionTargetBox="[315,1278,326,1774]" captionTargetId="figure@41.[315,1278,326,1775]" captionTargetPageId="41" captionText="FIGURE 27. Philocelis robrochai sp. nov.; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs." httpUri="https://zenodo.org/record/174313/files/figure.png" pageId="42" pageNumber="43">Figs. 27</figureCitation>
B, 28A). Mouth opening on ventral surface, middle of body. Digestive central syncytium extends from frontal glands posteriorly to male copulatory apparatus.
</paragraph>
<caption id="DF08664AE606FF97FE84113BFD38FA77" httpUri="https://zenodo.org/record/174315/files/figure.png" pageId="43" pageNumber="44" targetBox="[264,1322,406,1309]" targetPageId="43">
<paragraph id="8BC836C2E606FF97FE84113BFD38FA77" blockId="43.[264,1323,1333,1426]" pageId="43" pageNumber="44">
<emphasis id="B903EAD0E606FF97FE84113BFE35FAA8" bold="true" box="[264,418,1333,1357]" pageId="43" pageNumber="44">FIGURE 29.</emphasis>
<taxonomicName id="4C774D41E606FF97FE3F1138FD00FAA8" box="[435,663,1334,1357]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="43" pageNumber="44" phylum="Xenacoelomorpha" rank="species" species="robrochai" status="sp. nov.">
<emphasis id="B903EAD0E606FF97FE3F1138FD00FAA8" box="[435,663,1334,1357]" italics="true" pageId="43" pageNumber="44">Philocelis robrochai</emphasis>
</taxonomicName>
<emphasis id="B903EAD0E606FF97FD2B113BFC91FAA8" bold="true" box="[679,774,1333,1357]" pageId="43" pageNumber="44">
<taxonomicNameLabel id="A23057ABE606FF97FD2B113BFC91FAA8" box="[679,774,1333,1357]" pageId="43" pageNumber="44" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
; whole-mounts stained with Alexa-488-labeled phalloidin and viewed with confocal microscopy. A. Ventral projection of posterior end of animal. B. Projection of male copulatory organ.
</paragraph>
</caption>
<paragraph id="8BC836C2E606FF97FEB411C8FED5F935" blockId="43.[264,1323,1478,1904]" pageId="43" pageNumber="44">Body-wall musculature with circular muscles that encircle the body along entire length of animal; straight longitudinal muscles present between frontal organ and anterior edge of mouth; longitudinal muscles with a longitudinal orientation anteriorly that bend medially to cross diagonally over the body (longitudinal-cross-over fibers) present in dorsal and ventral body walls; longitudinal muscles in the anterior half of the body that wrap around the posterior rim of the mouth (U-shaped muscles) present in ventral body wall.</paragraph>
<paragraph id="8BC836C2E606FF97FEB412D0FD63F8AD" blockId="43.[264,1323,1478,1904]" pageId="43" pageNumber="44">
Ovaries paired, ventral; extend from frontal glands posteriorly to bursal nozzle (
<figureCitation id="134C2A47E606FF97FB7C12D0FEBFF8C5" captionStart="FIGURE 27" captionStartId="41.[264,367,1796,1820]" captionTargetBox="[315,1278,326,1774]" captionTargetId="figure@41.[315,1278,326,1775]" captionTargetPageId="41" captionText="FIGURE 27. Philocelis robrochai sp. nov.; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs." httpUri="https://zenodo.org/record/174313/files/figure.png" pageId="43" pageNumber="44">Figs. 27</figureCitation>
B, D, 28A). Testes paired, dorsal; separate from ovary. Testes extend from frontal gland posteriorly to seminal vesicles (
<figureCitation id="134C2A47E606FF97FDF01320FD45F8AD" box="[636,722,1838,1864]" captionStart="FIGURE 28" captionStartId="42.[264,367,1495,1519]" captionTargetBox="[273,1314,327,1462]" captionTargetId="figure@42.[273,1315,326,1471]" captionTargetPageId="42" captionText="FIGURE 28. Philocelis robrochai sp. nov.; reconstructions to show arrangement of organs. A. Sagittal reconstruction showing arrangement of female copulatory organ. B. Sagittal reconstruction showing arrangement of male copulatory organ." httpUri="https://zenodo.org/record/174314/files/figure.png" pageId="43" pageNumber="44">Fig. 28</figureCitation>
B).
</paragraph>
<paragraph id="8BC836C2E606FF90FEB41358FBC2FED3" blockId="43.[264,1323,1478,1904]" lastBlockId="44.[264,1323,284,1230]" lastPageId="44" lastPageNumber="45" pageId="43" pageNumber="44">
Common gonopore positioned ventrally at posterior end of body; opens directly to male copulatory organ anteriorly, and to vagina caudally (
<figureCitation id="134C2A47E601FF90FC211512FB9AFED3" box="[941,1037,284,310]" captionStart="FIGURE 28" captionStartId="42.[264,367,1495,1519]" captionTargetBox="[273,1314,327,1462]" captionTargetId="figure@42.[273,1315,326,1471]" captionTargetPageId="42" captionText="FIGURE 28. Philocelis robrochai sp. nov.; reconstructions to show arrangement of organs. A. Sagittal reconstruction showing arrangement of female copulatory organ. B. Sagittal reconstruction showing arrangement of male copulatory organ." httpUri="https://zenodo.org/record/174314/files/figure.png" pageId="44" pageNumber="45">Figs. 28</figureCitation>
A, B).
</paragraph>
<paragraph id="8BC836C2E601FF90FE84154AFE08FE33" blockId="44.[264,1323,284,1230]" pageId="44" pageNumber="45">
Male copulatory organ composed of a long (~120 µm), curved, conical sclerotized stylet surrounded by a weakly muscular penis sac (
<figureCitation id="134C2A47E601FF90FCAB1562FC1CFE63" box="[807,907,364,390]" captionStart="FIGURE 27" captionStartId="41.[264,367,1796,1820]" captionTargetBox="[315,1278,326,1774]" captionTargetId="figure@41.[315,1278,326,1775]" captionTargetPageId="41" captionText="FIGURE 27. Philocelis robrochai sp. nov.; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs." httpUri="https://zenodo.org/record/174313/files/figure.png" pageId="44" pageNumber="45">Figs. 27</figureCitation>
B, D, 28B, 29A, B). Penis flanked laterally by muscular seminal vesicles with long muscular ducts that connect to proximal end of penis.
</paragraph>
<paragraph id="8BC836C2E601FF90FEB415EAFCC9FDAB" blockId="44.[264,1323,284,1230]" pageId="44" pageNumber="45">
Vagina with thick wall; positioned dorsal to male copulatory organ; extends anteriorly to well-developed seminal bursa (
<figureCitation id="134C2A47E601FF90FD271602FC85FDC3" box="[683,786,524,550]" captionStart="FIGURE 27" captionStartId="41.[264,367,1796,1820]" captionTargetBox="[315,1278,326,1774]" captionTargetId="figure@41.[315,1278,326,1775]" captionTargetPageId="41" captionText="FIGURE 27. Philocelis robrochai sp. nov.; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs." httpUri="https://zenodo.org/record/174313/files/figure.png" pageId="44" pageNumber="45">Figs. 27</figureCitation>
D, 28A). Bursa with bursal nozzle directed ventrally and slightly posteriorly (
<figureCitation id="134C2A47E601FF90FD1B163AFD6FFDAB" box="[663,760,564,590]" captionStart="FIGURE 27" captionStartId="41.[264,367,1796,1820]" captionTargetBox="[315,1278,326,1774]" captionTargetId="figure@41.[315,1278,326,1775]" captionTargetPageId="41" captionText="FIGURE 27. Philocelis robrochai sp. nov.; photomicrographs of living specimens. A. Dorsal view of unsqueezed specimen. B. Dorsal view of squeezed specimen. C. Rhabdoid glands within epidermis. D. Dorsal view of copulatory organs." httpUri="https://zenodo.org/record/174313/files/figure.png" pageId="44" pageNumber="45">Figs. 27</figureCitation>
D, 28A).
</paragraph>
</subSubSection>
<subSubSection id="C36D6549E601FF90FEB41655FEC9FB2B" pageId="44" pageNumber="45" type="discussion">
<paragraph id="8BC836C2E601FF90FEB41655FEC9FB2B" blockId="44.[264,1323,284,1230]" pageId="44" pageNumber="45">
<emphasis id="B903EAD0E601FF90FEB41655FE3DFD90" bold="true" box="[312,426,603,629]" pageId="44" pageNumber="45">Remarks</emphasis>
. Species of the genus
<taxonomicName id="4C774D41E601FF90FD3B1652FCBBFD90" box="[695,812,604,629]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="genus">
<emphasis id="B903EAD0E601FF90FD3B1652FCBBFD90" box="[695,812,604,629]" italics="true" pageId="44" pageNumber="45">Philocelis</emphasis>
</taxonomicName>
are united in having the vagina positioned posterior to the male copulatory organ, a seminal bursa with attached bursal nozzle, and a male copulatory organ composed of a muscular penis-sac surrounding sclerotized stylets. Although the penis stylet of
<taxonomicName id="4C774D41E601FF90FDED16DAFD63FD08" box="[609,756,724,749]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="species" species="robrochai">
<emphasis id="B903EAD0E601FF90FDED16DAFD63FD08" box="[609,756,724,749]" italics="true" pageId="44" pageNumber="45">P. robrochai</emphasis>
</taxonomicName>
is more compact than that of
<taxonomicName id="4C774D41E601FF90FBEE16DAFD8AFCF3" authority="Hooge and Tyler, 2003" authorityName="Hooge and Tyler" authorityYear="2003" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="species" species="brueggemanni">
<emphasis id="B903EAD0E601FF90FBEE16DAFABDFD08" box="[1122,1322,724,749]" italics="true" pageId="44" pageNumber="45">P. brueggemanni</emphasis>
Hooge and Tyler, 2003
</taxonomicName>
,
<taxonomicName id="4C774D41E601FF90FDA016F2FCD7FCF3" authority="Dorjes, 1968" authorityName="Dorjes" authorityYear="1968" box="[556,832,764,790]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="species" species="cellata">
<emphasis id="B903EAD0E601FF90FDA016F2FD0CFCF0" box="[556,667,764,789]" italics="true" pageId="44" pageNumber="45">P. cellata</emphasis>
<bibRefCitation id="EFE64B33E601FF90FD2816F2FCD7FCF3" author="Dorjes" box="[676,832,764,790]" pageId="44" pageNumber="48" refString="Dorjes, J. (1968) Die Acoela (Turbellaria) der deutschen Nordseekuste und ein neues System der Ordnung. Zeitschrift fr Zoologische Systematik und Evolutionsforschung, 6, 56 - 452." type="journal article" year="1968">Dörjes, 1968</bibRefCitation>
</taxonomicName>
, and
<taxonomicName id="4C774D41E601FF90FC0E16F2FB42FCF3" authority="Westblad, 1946" authorityName="Westblad" authorityYear="1946" box="[898,1237,764,790]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="species" species="karlingi">
<emphasis id="B903EAD0E601FF90FC0E16F2FC69FCF0" box="[898,1022,764,789]" italics="true" pageId="44" pageNumber="45">P. karlingi</emphasis>
(
<bibRefCitation id="EFE64B33E601FF90FB8316F2FB5AFCF3" author="Westblad" box="[1039,1229,764,790]" pageId="44" pageNumber="50" refString="Westblad, E. (1946) Studien uber skandinavische Turbellaria Acoela. IV. Arkiv for Zoologi, 38 A, 1 - 56." type="journal article" year="1946">Westblad, 1946</bibRefCitation>
)
</taxonomicName>
, which have widely spaced stylet needles, the overall organization of the male copulatory organ appears to be homologous. Preliminary analysis of both cytochrome oxidase I and 18S rDNA molecular sequence data support a strong relationship between
<taxonomicName id="4C774D41E601FF90FBC5177AFB4DFC68" box="[1097,1242,884,909]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="species" species="robrochai">
<emphasis id="B903EAD0E601FF90FBC5177AFB4DFC68" box="[1097,1242,884,909]" italics="true" pageId="44" pageNumber="45">P. robrochai</emphasis>
</taxonomicName>
and
<taxonomicName id="4C774D41E601FF90FA99177AFE26FC50" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="species" species="brueggemanni">
<emphasis id="B903EAD0E601FF90FA99177AFE26FC50" italics="true" pageId="44" pageNumber="45">P. brueggemanni</emphasis>
</taxonomicName>
(unpublished data). Interestingly, 18S sequence data also supports a close relationship between
<taxonomicName id="4C774D41E601FF90FD9B17CAFD1BFC38" box="[535,652,964,989]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="genus">
<emphasis id="B903EAD0E601FF90FD9B17CAFD1BFC38" box="[535,652,964,989]" italics="true" pageId="44" pageNumber="45">Philocelis</emphasis>
</taxonomicName>
species and
<taxonomicName id="4C774D41E601FF90FCCC17CAFE73FBE3" authority="Graff, 1882" authorityName="Graff" authorityYear="1882" class="Acoelomorpha" family="Actinoposthiidae" genus="Philactinoposthia" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="species" species="saliens">
<emphasis id="B903EAD0E601FF90FCCC17CAFBE4FC38" box="[832,1139,964,989]" italics="true" pageId="44" pageNumber="45">Philactinoposthia saliens</emphasis>
(Graff, 1882) (Actinoposthiidae)
</taxonomicName>
—a relationship that is further supported by similarities in sperm ultrastructure (
<bibRefCitation id="EFE64B33E601FF90FE3B101AFD0CFBCB" author="Petrov" box="[439,667,1044,1070]" pageId="44" pageNumber="50" refString="Petrov, A., Hooge, M., &amp; Tyler, S. (2004) Ultrastructure of sperms in Acoela (Acoelomorpha) and its concordance with molecular systematics. Invertebrate Biology, 123, 183 - 197." type="journal article" year="2004">Petrov et al. 2004</bibRefCitation>
). Several species of
<taxonomicName id="4C774D41E601FF90FC17101AFBFCFBC8" box="[923,1131,1044,1069]" class="Acoelomorpha" family="Actinoposthiidae" genus="Philactinoposthia" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="genus">
<emphasis id="B903EAD0E601FF90FC17101AFBFCFBC8" box="[923,1131,1044,1069]" italics="true" pageId="44" pageNumber="45">Philactinoposthia</emphasis>
</taxonomicName>
appear to have male copulatory organs with a similar composition to that of
<taxonomicName id="4C774D41E601FF90FC591032FBDDFBB0" box="[981,1098,1084,1109]" class="Acoelomorpha" family="Otocelididae" genus="Philocelis" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="genus">
<emphasis id="B903EAD0E601FF90FC591032FBDDFBB0" box="[981,1098,1084,1109]" italics="true" pageId="44" pageNumber="45">Philocelis</emphasis>
</taxonomicName>
, and given that the
<taxonomicName id="4C774D41E601FF90FE84106AFE0AFB9B" box="[264,413,1124,1150]" class="Acoelomorpha" family="Otocelididae" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="family">Otocelididae</taxonomicName>
has been shown to be non-monophyletic (
<bibRefCitation id="EFE64B33E601FF90FC17106AFB0DFB9B" author="Hooge" box="[923,1178,1124,1150]" pageId="44" pageNumber="49" refString="Hooge, M. D. &amp; Tyler, S. (2005) New tools for resolving phylogenies: a systematic revision of the Convolutidae (Acoelomorpha, Acoela). Journal of Zoological Systematics and Evolutionary Research, 43, 100 - 113." type="journal article" year="2005">Hooge &amp; Tyler 2005</bibRefCitation>
), any future revision of the
<taxonomicName id="4C774D41E601FF90FE3B1082FDDAFB43" box="[439,589,1164,1190]" class="Acoelomorpha" family="Otocelididae" kingdom="Animalia" order="Acoela" pageId="44" pageNumber="45" phylum="Xenacoelomorpha" rank="family">Otocelididae</taxonomicName>
should include an examination of the relationship of these two genera.
</paragraph>
</subSubSection>
</treatment>
</document>