276 lines
70 KiB
XML
276 lines
70 KiB
XML
<document id="4D4D100608CB4B4745DCBD94B85443AC" ID-DOI="10.1093/zoolinnean/zlad015" ID-ISSN="0024-4082" ID-Zenodo-Dep="8426115" ID-ZooBank="854433BD-5D76-44C1-9BAB-CAC00F5131C9" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1696940089814" checkinUser="plazi" docAuthor="Li, Ran, Zhuang, Wenbao, Feng, Xiaochen, Al-Farraj, Saleh A., Schrecengost, Anna, Roưerova, Johana, Beinart, Roxanne A. & Hu, Xiaozhong" docDate="2023" docId="0397E660DC5E831DFEB440FDF7ADFA2C" docLanguage="en" docName="zlad015.pdf" docOrigin="Zoological Journal of the Linnean Society 199 (2)" docSource="http://dx.doi.org/10.1093/zoolinnean/zlad015" docStyle="DocumentStyle:4F230B9370E98E256D973D6DFB57F36C.1:ZoolJLinnSoc.2023-.journal_article" docStyleId="4F230B9370E98E256D973D6DFB57F36C" docStyleName="ZoolJLinnSoc.2023-.journal_article" docStyleVersion="1" docTitle="Plagiopyla rariseta Li & Zhuang & Feng & Al-Farraj & Schrecengost & Roưerova & Beinart & Hu 2023, sp.nov." docType="treatment" docVersion="2" lastPageNumber="499" masterDocId="FFAE9E18DC58831BFF8A435DF21CFFA1" masterDocTitle="Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America" masterLastPageNumber="510" masterPageNumber="493" pageNumber="499" updateTime="1696940523517" updateUser="ExternalLinkService">
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<mods:title id="B501A0C010F9F07AC8893175215A2A53">Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America</mods:title>
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<mods:namePart id="395B69709563C3F76385B5588259C7D6">Li, Ran</mods:namePart>
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<mods:affiliation id="56248B8A962BA9C534F94094CA3BBEDF">College of Fisheries, & Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, ºingdao 266003, China & Institute of Evolution and Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
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<mods:roleTerm id="52F4591BFDCA6FDFA8E597C3F990FD54">Author</mods:roleTerm>
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<mods:namePart id="F087AFA46CCA9C2224CEA1CBD0523128">Zhuang, Wenbao</mods:namePart>
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<mods:affiliation id="79AC01B1208BDE86320EF813A485E167">College of Fisheries, & Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, ºingdao 266003, China & Institute of Evolution and Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
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<mods:namePart id="AAD5FB48B8A1AFAD276AB130E483F590">Feng, Xiaochen</mods:namePart>
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<mods:affiliation id="8DCE4CAC0F6895359C9C9C14389DC28C">College of Fisheries, & Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, ºingdao 266003, China & Institute of Evolution and Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
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<mods:namePart id="B2018A7EFC1CEB5CE375DB2059BCB9FB">Al-Farraj, Saleh A.</mods:namePart>
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<mods:affiliation id="75DD1FA309F878C00131515D244B55C5">Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia</mods:affiliation>
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</mods:name>
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<mods:namePart id="ADC38C89CFA3939D7D4A377563890126">Schrecengost, Anna</mods:namePart>
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<mods:affiliation id="A6C69FA25FD64EFCC63FD0BB19503218">Graduate School of Oceanography, University of Rhode Island, 215 South Ferry Rd, Narraganseư, Rhode Island 02882, USA</mods:affiliation>
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<mods:namePart id="CAC5F5AE880313EADADCE20445441495">Roưerova, Johana</mods:namePart>
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<mods:affiliation id="39205BE8158A1FD6D3503BAF7F476A81">Graduate School of Oceanography, University of Rhode Island, 215 South Ferry Rd, Narraganseư, Rhode Island 02882, USA</mods:affiliation>
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<mods:namePart id="547E4ADABC2E32E73FEED9C33F8DEF9F">Beinart, Roxanne A.</mods:namePart>
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<mods:affiliation id="4412C20603D24D52C223671982505C21">Graduate School of Oceanography, University of Rhode Island, 215 South Ferry Rd, Narraganseư, Rhode Island 02882, USA</mods:affiliation>
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<mods:namePart id="3A25B1C13D441420F2ED0114BA83E3A9">Hu, Xiaozhong</mods:namePart>
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<mods:affiliation id="06827EC58CBC6387BCBFEA10F8242B99">College of Fisheries, & Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, ºingdao 266003, China & Institute of Evolution and Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
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<mods:nameIdentifier id="CA459750D1DF2D6373DF445D57A72C0C" type="email">xiaozhonghu@ouc.edu.cn</mods:nameIdentifier>
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<mods:title id="BE4A29FEEDE63730E9E4C0210D7E2E37">Zoological Journal of the Linnean Society</mods:title>
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<mods:part id="DFDA6A48754545FDC567E09020F6F369">
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<mods:date id="2FE9CAED5A95B5B50094E2ADD6A6CC32">2023</mods:date>
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<mods:number id="624BCC4CA2A51797229BDD811CFEAAA7">2023-07-28</mods:number>
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<mods:number id="A68D489D44CEF4A83082F6A39C729C97">199</mods:number>
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<mods:url id="8BBDB8FC15E559A1F316ACD96148F970">http://dx.doi.org/10.1093/zoolinnean/zlad015</mods:url>
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<mods:identifier id="3E1AD97BDB79000FFE49208BB0F3D2BF" type="ISSN">0024-4082</mods:identifier>
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<treatment id="0397E660DC5E831DFEB440FDF7ADFA2C" ID-DOI="http://doi.org/10.5281/zenodo.8426119" ID-Zenodo-Dep="8426119" LSID="urn:lsid:plazi:treatment:0397E660DC5E831DFEB440FDF7ADFA2C" httpUri="http://treatment.plazi.org/id/0397E660DC5E831DFEB440FDF7ADFA2C" lastPageNumber="499" pageId="6" pageNumber="499">
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<paragraph id="8B815776DC5E831DFEB440FDF050FC1A" blockId="6.[318,588,928,955]" box="[318,588,928,955]" pageId="6" pageNumber="499">
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<heading id="D0C9E01ADC5E831DFEB440FDF050FC1A" bold="true" box="[318,588,928,955]" centered="true" fontSize="11" level="2" pageId="6" pageNumber="499" reason="2">
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<emphasis id="B94A8B64DC5E831DFEB440FDF050FC1A" bold="true" box="[318,588,928,955]" pageId="6" pageNumber="499">
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<taxonomicName id="4C3E2CF5DC5E831DFEB440FDF3E0FC1B" authority="Li & Zhuang & Feng & Al-Farraj & Schrecengost & Roưerova & Beinart & Hu, 2023" authorityName="Li & Zhuang & Feng & Al-Farraj & Schrecengost & Roưerova & Beinart & Hu" authorityYear="2023" box="[318,508,928,955]" class="Kinetofragminophora" family="Plagiopylidae" genus="Plagiopyla" kingdom="Chromista" order="Plagiopylida" pageId="6" pageNumber="499" phylum="Ciliophora" rank="species" species="rariseta" status="sp.nov.">
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<emphasis id="B94A8B64DC5E831DFEB440FDF3E0FC1B" bold="true" box="[318,508,928,955]" italics="true" pageId="6" pageNumber="499">Plagiopyla rariseta</emphasis>
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</taxonomicName>
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<taxonomicNameLabel id="A279361FDC5E831DFD8A40FCF050FC1A" box="[512,588,929,955]" pageId="6" pageNumber="499" rank="species">sp.nov.</taxonomicNameLabel>
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</emphasis>
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</heading>
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<subSubSection id="C32404FDDC5E831DFEE9408CF7ADFA2C" pageId="6" pageNumber="499" type="description">
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<paragraph id="8B815776DC5E831DFEE9408CF03BFC4A" blockId="6.[355,551,977,1004]" box="[355,551,977,1004]" pageId="6" pageNumber="499">
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<emphasis id="B94A8B64DC5E831DFEE9408CF03BFC4A" bold="true" box="[355,551,977,1004]" pageId="6" pageNumber="499">
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(
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<figureCitation id="13054BF3DC5E831DFEE4408CF3B7FC4A" box="[366,427,977,1003]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4</figureCitation>
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,
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<figureCitation id="13054BF3DC5E831DFE3F408FF3DFFC4D" box="[437,451,978,1004]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5</figureCitation>
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;
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<tableCitation id="C6BC62CDDC5E831DFE45408FF000FC4A" box="[463,540,977,1004]" captionStart="Table 3" captionStartId="9.[113,168,143,167]" captionText="Table 3. Morphometric characteristics of the Chinese populations of Plagiopyla rariseta (BAINIDI; upper line) and Plagiopyla flontata (SHAZIKOU; lower line)." pageId="6" pageNumber="499">Table 3</tableCitation>
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)
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</emphasis>
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</paragraph>
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<paragraph id="8B815776DC5E831DFF0B474FF3EBFBE8" blockId="6.[129,777,1042,1097]" pageId="6" pageNumber="499">
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<emphasis id="B94A8B64DC5E831DFF0B474FF379FB8B" box="[129,357,1042,1066]" italics="true" pageId="6" pageNumber="499">ZooBank registration:</emphasis>
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<uri id="FFAF5B74DC5E831DFEF1474FF3EFFBE8" pageId="6" pageNumber="499">
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urn:lsid:zoobank.org:act:
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<uuid id="FF986DA3DC5E831DFDF7474FF3EFFBE8" pageId="6" pageNumber="499">DD5C54CE-052D-4C2B-BA78-89C3B604B104</uuid>
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</uri>
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.
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</paragraph>
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<paragraph id="8B815776DC5E831DFF0B4736F0BDFADC" blockId="6.[129,779,1131,1405]" pageId="6" pageNumber="499">
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<emphasis id="B94A8B64DC5E831DFF0B4736F2FEFB23" box="[129,226,1131,1154]" italics="true" pageId="6" pageNumber="499">Diagnosis:</emphasis>
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Cell size 55–80 × 25–45 μm
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<emphasis id="B94A8B64DC5E831DFD984736F04EFB23" box="[530,594,1131,1154]" italics="true" pageId="6" pageNumber="499">in vivo</emphasis>
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, shape ellipsoidal. Length to width ratio 1.4–2.2:
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<quantity id="4CC6FA93DC5E831DFE2147D7F3D7FB03" box="[427,459,1162,1186]" metricMagnitude="-2" metricUnit="m" metricValue="2.54" pageId="6" pageNumber="499" unit="in" value="1.0">
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1
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<emphasis id="B94A8B64DC5E831DFE3247D6F3D7FB03" box="[440,459,1163,1186]" italics="true" pageId="6" pageNumber="499">in</emphasis>
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</quantity>
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||
vivo. 50–58 monokinetidal somatic kineties. Cilia above the oral opening loosely arranged. One globular to slightly ellipsoidal macronucleus and one small, spherical micronucleus. Striated band about 32 μm long, extending to below midbody. One dense ciliary row. Numerous curved extrusomes, 8–11 µm long. Region above oral opening about 30% of cell length. Tube-shaped buccal cavity obliquely extended to the leħ dorsal side, about 80% of cell width, bending upwards in the middle.
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</paragraph>
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<paragraph id="8B815776DC5E831DFF0B46C2F2C7FA54" blockId="6.[129,777,1439,1525]" pageId="6" pageNumber="499">
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<emphasis id="B94A8B64DC5E831DFF0B46C2F2ECFA16" box="[129,240,1439,1463]" italics="true" pageId="6" pageNumber="499">Etymology:</emphasis>
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Composite of the Latin words
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<emphasis id="B94A8B64DC5E831DFDDB46C2F066FA17" box="[593,634,1439,1462]" italics="true" pageId="6" pageNumber="499">rara</emphasis>
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||
(few, sparse) and
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<emphasis id="B94A8B64DC5E831DFF2746E2F2C3FA77" box="[173,223,1471,1494]" italics="true" pageId="6" pageNumber="499">saeta</emphasis>
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(bristle ~ cilia), referring to sparse cilia above the oral opening.
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</paragraph>
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<paragraph id="8B815776DC5E831DFF0B454AF06CF9EF" blockId="6.[129,778,1559,1614]" pageId="6" pageNumber="499">
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<materialsCitation id="3B565D2BDC5E831DFF0B454AF070F9EF" country="China" latitude="36.200863" location="Sediments" longLatPrecision="1" longitude="120.37425" municipality="Bainidi Park" pageId="6" pageNumber="499" specimenCount="1" typeStatus="holotype">
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<typeStatus id="5485E9D4DC5E831DFF0B454AF2ADF98F" box="[129,177,1559,1582]" pageId="6" pageNumber="499">Type</typeStatus>
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locality:
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</emphasis>
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<location id="8EE101ADDC5E831DFEAB454AF390F98E" LSID="urn:lsid:plazi:treatment:0397E660DC5E831DFEB440FDF7ADFA2C:8EE101ADDC5E831DFEAB454AF390F98E" box="[289,396,1559,1583]" country="China" latitude="36.200863" longLatPrecision="1" longitude="120.37425" municipality="Bainidi Park" name="Sediments" pageId="6" pageNumber="499">Sediments</location>
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||
of intertidal zone in
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<collectingMunicipality id="6BE5CD0CDC5E831DFD0A454AF11AF98E" box="[640,774,1559,1583]" pageId="6" pageNumber="499">Bainidi Park</collectingMunicipality>
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, ºingdao,
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<collectingCountry id="F32917E6DC5E831DFF6E456BF33FF9EF" box="[228,291,1590,1614]" name="China" pageId="6" pageNumber="499">China</collectingCountry>
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(
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;
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<geoCoordinate id="EE0A31B1DC5E831DFE46456BF079F9EF" box="[460,613,1589,1614]" degrees="120" direction="east" minutes="22" orientation="longitude" pageId="6" pageNumber="499" precision="1" seconds="27.3" value="120.37425">120°22ʹ27.3″E</geoCoordinate>
|
||
)
|
||
</materialsCitation>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="8B815776DC5E831DFF0B452DF076F8E2" blockId="6.[129,778,1647,1859]" pageId="6" pageNumber="499">
|
||
<emphasis id="B94A8B64DC5E831DFF0B452DF336F926" box="[129,298,1647,1671]" italics="true" pageId="6" pageNumber="499">Type deposition:</emphasis>
|
||
<materialsCitation id="3B565D2BDC5E831DFEB14532F07AF8E2" country="China" location="Laboratory of Protozoology" pageId="6" pageNumber="499" specimenCount="4" typeStatus="holotype">
|
||
The protargol slide (registration number: LR2020071701-01) with the
|
||
<typeStatus id="5485E9D4DC5E831DFE2645D2F014F906" box="[428,520,1679,1703]" pageId="6" pageNumber="499">holotype</typeStatus>
|
||
specimen (
|
||
<figureCitation id="13054BF3DC5E831DFDF445D2F0FCF907" box="[638,736,1679,1703]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4H, I</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFD6045D2F288F967" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5F, G</figureCitation>
|
||
) and several
|
||
<typeStatus id="5485E9D4DC5E831DFE9745F2F36AF966" box="[285,374,1711,1735]" pageId="6" pageNumber="499">paratype</typeStatus>
|
||
specimens, and another slide (registration number: LR2020071701-02) with several
|
||
<typeStatus id="5485E9D4DC5E831DFDE74593F0DAF947" box="[621,710,1742,1766]" pageId="6" pageNumber="499">paratype</typeStatus>
|
||
specimens were deposited in the
|
||
<location id="8EE101ADDC5E831DFE1545B0F0A1F8A4" LSID="urn:lsid:plazi:treatment:0397E660DC5E831DFEB440FDF7ADFA2C:8EE101ADDC5E831DFE1545B0F0A1F8A4" box="[415,701,1773,1797]" country="China" name="Laboratory of Protozoology" pageId="6" pageNumber="499">Laboratory of Protozoology</location>
|
||
, Ocean University of
|
||
<collectingCountry id="F32917E6DC5E831DFE804451F357F885" box="[266,331,1804,1828]" name="China" pageId="6" pageNumber="499">China</collectingCountry>
|
||
, ºingdao,
|
||
<collectingCountry id="F32917E6DC5E831DFE324451F3E5F885" box="[440,505,1804,1828]" name="China" pageId="6" pageNumber="499">China</collectingCountry>
|
||
. The
|
||
<typeStatus id="5485E9D4DC5E831DFDA54451F097F885" box="[559,651,1804,1828]" pageId="6" pageNumber="499">holotype</typeStatus>
|
||
was marked by a black ink circle on the back side of the slide
|
||
</materialsCitation>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="8B815776DC5E831DFF0B443EF788FD5A" blockId="6.[129,778,1891,1977]" lastBlockId="6.[825,1475,144,1421]" pageId="6" pageNumber="499">
|
||
<emphasis id="B94A8B64DC5E831DFF0B443EF01EF8DA" box="[129,514,1891,1915]" italics="true" pageId="6" pageNumber="499">Description of Chinese population:</emphasis>
|
||
Body non-contractile, 55–80 × 25–45 μm
|
||
<emphasis id="B94A8B64DC5E831DFEC644DEF390F83B" box="[332,396,1923,1946]" italics="true" pageId="6" pageNumber="499">in vivo</emphasis>
|
||
, with length to width ratio 1.4–2.2:1. Shape ellipsoidal in both living and stained cells, dorsoventrally flaưened (
|
||
<figureCitation id="13054BF3DC5E831DFC2E43CDF1EEFF09" box="[932,1010,144,168]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4A</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFB8A43CDF626FF06" box="[1024,1082,144,168]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5A, B</figureCitation>
|
||
). Cortex ridged with kinetosomes located at top of ridges (
|
||
<figureCitation id="13054BF3DC5E831DFB9743F2F67BFF66" box="[1053,1127,175,199]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">Fig. 5K</figureCitation>
|
||
). Cytoplasm colourless, including numerous small globular granules and a few food vacuoles with green algae inside (
|
||
<figureCitation id="13054BF3DC5E831DFB8843B3F64DFEA7" box="[1026,1105,238,262]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4A</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFBD543B3F6A9FEA4" box="[1119,1205,238,262]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5A, B, L</figureCitation>
|
||
). One globular to slightly ellipsoidal macronucleus, 18–23 × 18–20 µm
|
||
<emphasis id="B94A8B64DC5E831DFAB74253F796FE84" box="[1341,1418,270,293]" italics="true" pageId="6" pageNumber="499">in vivo</emphasis>
|
||
and 13–22 × 12–21 µm aħer staining, mostly located at midbody (
|
||
<figureCitation id="13054BF3DC5E831DFCCE4211F1B3FEC2" box="[836,943,332,356]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4A, E</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFC364211F1CBFEC5" box="[956,983,332,356]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5E</figureCitation>
|
||
). Single globular micronucleus, relatively small, 1.1–1.7 µm across in stained specimens, always slightly enveloped by macronucleus, hardly observed
|
||
<emphasis id="B94A8B64DC5E831DFB2B42D6F6FFFE03" box="[1185,1251,395,418]" italics="true" pageId="6" pageNumber="499">in vivo</emphasis>
|
||
(
|
||
<figureCitation id="13054BF3DC5E831DFB7F42D7F721FE03" box="[1269,1341,394,418]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">Fig. 5E</figureCitation>
|
||
). Numerous curved extrusomes 8–11 µm long, distributed randomly in endoplasm and relatively densely under the cortex (
|
||
<figureCitation id="13054BF3DC5E831DFA944294F796FE40" box="[1310,1418,457,481]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4A, B</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFA1D4294F7AEFE40" box="[1431,1458,457,481]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5L</figureCitation>
|
||
). Extruded extrusomes slender, needle-shaped, 20–37 µm long (
|
||
<figureCitation id="13054BF3DC5E831DFCCE4155F18CFD81" box="[836,912,520,544]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4C</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFC114155F1A5FD81" box="[923,953,520,544]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5C</figureCitation>
|
||
). Single subterminal contractile vacuole, opens dorsally with two distinct pores (
|
||
<figureCitation id="13054BF3DC5E831DFBE0417AF6CBFD9E" box="[1130,1239,551,575]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4A, D</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFB69417AF740FD9E" box="[1251,1372,551,575]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5A, B, K, N</figureCitation>
|
||
). Striated band on dorsal surface, 29–35 µm long, 3–4 µm wide, extending below midlength. In normal cells, striated band usually rodshaped viewed from dorsal side, parallel to ciliary rows, loop at the front part easily observed in compressed cells (
|
||
<figureCitation id="13054BF3DC5E831DFADE41F9F7A1FD1D" box="[1364,1469,676,700]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4D, I</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFCB3419EF146FD7A" box="[825,858,707,731]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5O</figureCitation>
|
||
). Swims by spiralling counter-clockwise around body axis. Sluggish or even motionless aħer a period of exposure to air.
|
||
</paragraph>
|
||
<paragraph id="8B815776DC5E831DFCDF405FF700FBB4" blockId="6.[825,1475,144,1421]" pageId="6" pageNumber="499">
|
||
Somatic cilia 10–15 µm long, uniformly packed in 50–58 monokinetidal longitudinal kineties (25–31 on ventral, 25–30 on dorsal), except for about two dikinetid-like structures right to frontal region of striated band (
|
||
<figureCitation id="13054BF3DC5E831DFBFD403DF6E5FCD9" box="[1143,1273,864,888]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4G, H, I</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFA89403DF726FCD9" box="[1283,1338,864,888]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5F, G</figureCitation>
|
||
). Cilia above the oral opening arranged loosely (
|
||
<figureCitation id="13054BF3DC5E831DFB2E40DDF6EAFC39" box="[1188,1270,896,920]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4H</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFA894022F701FC36" box="[1283,1309,895,919]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5F</figureCitation>
|
||
). Seven to nine caudal cilia positioned at leħ rear margin, rigid, around 10 µm long, oriented to lower leħ (
|
||
<figureCitation id="13054BF3DC5E831DFBDE40E3F6BCFC77" box="[1108,1184,958,982]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4A</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFB2140E3F6D3FC77" box="[1195,1231,958,982]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5M</figureCitation>
|
||
). One dense ciliary row (DC), 8–11 µm long, comprised of 10–16 kinetosomes, located leħ to cytoproct on dorsal side (
|
||
<figureCitation id="13054BF3DC5E831DFBF540A0F6FDFBB5" box="[1151,1249,1021,1045]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4D, I</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFB6640A0F710FBB4" box="[1260,1292,1021,1045]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5G</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B815776DC5E831DFCDF4741F7ADFA2C" blockId="6.[825,1475,144,1421]" pageId="6" pageNumber="499">
|
||
Oral opening slit-shaped, opened at right ventral surface, surrounded by two lip-like structures (
|
||
<figureCitation id="13054BF3DC5E831DFB104761F718FBF2" box="[1178,1284,1084,1108]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4A, H</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFA844766F790FBF2" box="[1294,1420,1083,1107]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5A, B, F, H, J</figureCitation>
|
||
). No obvious nose-like structure on right side of upper oral lip. Region above oral opening about 30% of cell length. Buccal cavity long and thick, obliquely extended to leħ dorsal side, forming a deep tube, about 80% of cell width, making an upwards bend in middle (
|
||
<figureCitation id="13054BF3DC5E831DFCCE4785F192FB51" box="[836,910,1240,1264]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4F</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFC104785F1F2FB51" box="[922,1006,1240,1264]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5D, H, I</figureCitation>
|
||
). Distance from anterior end of cell to top of buccal cavity 10–12 μm. Oral ciliature much denser than somatic one and discontinuous, leaving gaps around upper and lower lips, with upper gap 2–4 μm wide (
|
||
<figureCitation id="13054BF3DC5E831DFBFF466BF6DAFAEF" box="[1141,1222,1334,1358]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4H</figureCitation>
|
||
,
|
||
<figureCitation id="13054BF3DC5E831DFB59466BF6F1FAEF" box="[1235,1261,1334,1358]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5F</figureCitation>
|
||
). Oral kineties (51– 56) extended from exterior of oral cavity to interior (
|
||
<figureCitation id="13054BF3DC5E831DFADE4608F7A0FACC" box="[1364,1468,1365,1390]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4F, H</figureCitation>
|
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,
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<figureCitation id="13054BF3DC5E831DFCB34628F16DFA2D" box="[825,881,1397,1421]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5D, F</figureCitation>
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). Upper oral lip kineties 5–8 μm long (
|
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<figureCitation id="13054BF3DC5E831DFB734628F743FA2D" box="[1273,1375,1397,1421]" captionStart="Figure 4" captionStartId="7.[115,180,985,1009]" captionTargetBox="[149,1420,154,947]" captionTargetId="figure-510@7.[146,1426,144,950]" captionTargetPageId="7" captionText="Figure 4. Drawings of Plagiopyla rariseta from life (A–C, E) and aħer protargol staining (D, F–I). A, ventral view of a representative individual, arrowheads show caudal cilia. B, C, resting (B) and extruded (C) extrusomes. D, dorsal view, showing position of striated band, cytoproct (red arrow), dense ciliary row (black arrow) and contractile vacuole pores (arrowheads). E, different body shapes. F, details of oral region, showing oral opening and buccal cavity. G, details of dikinetids on right front side of striated band. H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrow indicates striated band, arrowhead shows dense ciliary row, red kinetosomes indicate dikinetids on right front side of striated band. Abbreviations:CV, contractile vacuole; Ex, extrusomes; SB, striated band. Scale bars: 30 μm (A, E, H, I), 25μm (C)." figureDoi="http://doi.org/10.5281/zenodo.8426125" httpUri="https://zenodo.org/record/8426125/files/figure.png" pageId="6" pageNumber="499">Figs 4F, H</figureCitation>
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,
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<figureCitation id="13054BF3DC5E831DFAE04628F7BDFA2D" box="[1386,1441,1397,1421]" captionStart="Figure 5" captionStartId="8.[129,194,1128,1152]" captionTargetBox="[162,1441,146,1100]" captionTargetId="figure-361@8.[160,1443,144,1101]" captionTargetPageId="8" captionText="Figure 5. Photomicrographs of Plagiopyla rariseta from life (A–C, H–O) and aħer protargol staining (D–G). A, B, ventral views of different individuals, showing different body shape.C, extruded extrusomes (arrowheads).D, details of oral opening and buccal cavity tube (arrowhead). E, macronucleus and slightly enveloped micronucleus (arrowhead). F, G, ventral (F) and dorsal (G) views of the holotype to show somatic and oral kineties, arrowhead shows dense ciliary row. H, I, oral region, showing buccal cavity (arrowheads) under different focal planes. J, ventral view of a slightly compressed cell. K, posterior region of cell, arrowhead indicates contractile vacuole, arrow marks cortical ridges.L, curved extrusomes (arrowheads) under the cortex.M, posterior end of cell, showing caudal cilia (arrowhead).N, contractile vacuole pores (arrowheads). O, dorsal view, arrowhead shows striated band. Scale bars: 30 μm." figureDoi="http://doi.org/10.5281/zenodo.8426127" httpUri="https://zenodo.org/record/8426127/files/figure.png" pageId="6" pageNumber="499">5D, F</figureCitation>
|
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).
|
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</paragraph>
|
||
</subSubSection>
|
||
</treatment>
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</document> |