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<document id="15E816934B920E3D496EF7061A87FAE6" ID-DOI="10.1093/zoolinnean/zlad015" ID-ISSN="0024-4082" ID-Zenodo-Dep="8426115" ID-ZooBank="854433BD-5D76-44C1-9BAB-CAC00F5131C9" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1696940089814" checkinUser="plazi" docAuthor="Li, Ran, Zhuang, Wenbao, Feng, Xiaochen, Al-Farraj, Saleh A., Schrecengost, Anna, Roưerova, Johana, Beinart, Roxanne A. &amp; Hu, Xiaozhong" docDate="2023" docId="0397E660DC5B831DFC6D45CBF118FCBA" docLanguage="en" docName="zlad015.pdf" docOrigin="Zoological Journal of the Linnean Society 199 (2)" docSource="http://dx.doi.org/10.1093/zoolinnean/zlad015" docStyle="DocumentStyle:4F230B9370E98E256D973D6DFB57F36C.1:ZoolJLinnSoc.2023-.journal_article" docStyleId="4F230B9370E98E256D973D6DFB57F36C" docStyleName="ZoolJLinnSoc.2023-.journal_article" docStyleVersion="1" docTitle="Trimyema foissneri Li &amp; Zhuang &amp; Feng &amp; Al-Farraj &amp; Schrecengost &amp; Roưerova &amp; Beinart &amp; Hu 2023, sp.nov." docType="treatment" docVersion="2" lastPageNumber="499" masterDocId="FFAE9E18DC58831BFF8A435DF21CFFA1" masterDocTitle="Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America" masterLastPageNumber="510" masterPageNumber="493" pageNumber="496" updateTime="1696940523517" updateUser="ExternalLinkService">
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<mods:title id="BB84F03F128B719D90D8640DC71E3F5F">Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America</mods:title>
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<mods:namePart id="DB5929D36D85052B02BD67BE55FA3DFF">Li, Ran</mods:namePart>
<mods:affiliation id="4E5679B9C4C163932A0AE68D0646A848">College of Fisheries, &amp; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, ºingdao 266003, China &amp; Institute of Evolution and Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
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<mods:namePart id="AACA6530969E64CA7DFC9FDD673F2BB1">Zhuang, Wenbao</mods:namePart>
<mods:affiliation id="637986D3B8B5822D47E1E4B461D36673">College of Fisheries, &amp; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, ºingdao 266003, China &amp; Institute of Evolution and Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
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<mods:namePart id="7BFFE32DDA65179302A133816B9B50C4">Feng, Xiaochen</mods:namePart>
<mods:affiliation id="74EDA43FB95737A4EDCCCFDCD3C21BFC">College of Fisheries, &amp; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, ºingdao 266003, China &amp; Institute of Evolution and Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
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<mods:namePart id="FFFD862E12110161A9008F4FED2E100B">Al-Farraj, Saleh A.</mods:namePart>
<mods:affiliation id="4FA3555E8889355287662B8906CC8FE4">Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia</mods:affiliation>
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<mods:namePart id="70748399FE892A47DC3FA23EC41D5E0C">Schrecengost, Anna</mods:namePart>
<mods:affiliation id="111B13D1BD08028D83FDBCDA3E240849">Graduate School of Oceanography, University of Rhode Island, 215 South Ferry Rd, Narraganseư, Rhode Island 02882, USA</mods:affiliation>
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<mods:namePart id="9728C42344D169F885661370FBAB8FFD">Roưerova, Johana</mods:namePart>
<mods:affiliation id="5DF3C8B7D85E720C04BFEA17A8D5F92B">Graduate School of Oceanography, University of Rhode Island, 215 South Ferry Rd, Narraganseư, Rhode Island 02882, USA</mods:affiliation>
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<mods:namePart id="0D981F7B27009797F4D47C4867F413C4">Beinart, Roxanne A.</mods:namePart>
<mods:affiliation id="6F3103D48A18EA321CF55F98E09EFA7E">Graduate School of Oceanography, University of Rhode Island, 215 South Ferry Rd, Narraganseư, Rhode Island 02882, USA</mods:affiliation>
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<mods:namePart id="B7A9B8785C079361BE90CD00E92BFC9F">Hu, Xiaozhong</mods:namePart>
<mods:affiliation id="D63FEA0FC1D4442349E0D8A484880354">College of Fisheries, &amp; Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, ºingdao 266003, China &amp; Institute of Evolution and Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
<mods:nameIdentifier id="B9B39FAF8CA3AC9B3C00302F6009E100" type="email">xiaozhonghu@ouc.edu.cn</mods:nameIdentifier>
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<mods:date id="5EE05F5178908312B598F6A6B869E4A9">2023</mods:date>
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<taxonomicName id="4C3E2CF5DC5B8318FC6D45CBF6B9F911" authority="Li &amp; Zhuang &amp; Feng &amp; Al-Farraj &amp; Schrecengost &amp; Roưerova &amp; Beinart &amp; Hu, 2023" authorityName="Li &amp; Zhuang &amp; Feng &amp; Al-Farraj &amp; Schrecengost &amp; Roưerova &amp; Beinart &amp; Hu" authorityYear="2023" box="[999,1189,1686,1712]" class="Kinetofragminophora" family="Trimyemidae" genus="Trimyema" kingdom="Chromista" order="Trichostomatida" pageId="3" pageNumber="496" phylum="Ciliophora" rank="species" species="foissneri" status="sp.nov.">
<emphasis id="B94A8B64DC5B8318FC6D45CBF6B9F911" bold="true" box="[999,1189,1686,1712]" italics="true" pageId="3" pageNumber="496">Trimyema foissneri</emphasis>
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<subSubSection id="C32404FDDC5B831DFB874598F118FCBA" lastPageId="6" lastPageNumber="499" pageId="3" pageNumber="496" type="description">
<paragraph id="8B815776DC5B8318FB874598F6CDF97E" blockId="3.[1037,1233,1733,1760]" box="[1037,1233,1733,1760]" pageId="3" pageNumber="496">
<emphasis id="B94A8B64DC5B8318FB874598F6CDF97E" bold="true" box="[1037,1233,1733,1760]" pageId="3" pageNumber="496">
(
<figureCitation id="13054BF3DC5B8318FB9D4598F649F97E" box="[1047,1109,1733,1759]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="3" pageNumber="496">Figs 2</figureCitation>
,
<figureCitation id="13054BF3DC5B8318FBD54598F671F97E" box="[1119,1133,1733,1759]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="3" pageNumber="496">3</figureCitation>
;
<tableCitation id="C6BC62CDDC5B8318FBF2459BF6DAF97E" box="[1144,1222,1733,1760]" captionStart="Table 2" captionStartId="5.[113,168,1304,1328]" captionText="Table 2. Morphometric characteristics of Trimyema foissneri, Chinese population (TANGDAO)." pageId="3" pageNumber="496">Table 2</tableCitation>
)
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<paragraph id="8B815776DC5B8318FCA0445BF6ACF89C" blockId="3.[810,1459,1798,1853]" pageId="3" pageNumber="496">
<emphasis id="B94A8B64DC5B8318FCA0445BF608F8BF" box="[810,1044,1798,1822]" italics="true" pageId="3" pageNumber="496">ZooBank registration:</emphasis>
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urn:lsid:zoobank.org:act:
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.
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<emphasis id="B94A8B64DC5B8318FCA04402F18EF8D7" box="[810,914,1887,1910]" italics="true" pageId="3" pageNumber="496">Diagnosis:</emphasis>
Cell size 2035 × 1020 μm
<emphasis id="B94A8B64DC5B8318FB4F4402F71AF8D7" box="[1221,1286,1887,1910]" italics="true" pageId="3" pageNumber="496">in vivo</emphasis>
, shape fusiform; 2632 longitudinal somatic ciliary rows, forming three oblique ciliary girdles, making a turn slightly more than 360°. Region below posterior end of last girdle about 36% of body length, with spare cilia inserted. Caudal cilium about half body length. Epaulet present, including two to four kineties on right dorsal side with four to six kinetosomes, respectively. Ventrolateral fragment comprised of three or four kinetosomes. Oral opening oval, about 30% of cell length. Oral ciliature composed of two semicircular kineties intersecting spatially and one short kinety.
</paragraph>
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<emphasis id="B94A8B64DC5C831FFF0B40A5F2C9FBB1" bold="true" box="[129,213,1016,1040]" pageId="4" pageNumber="497">Figure 2.</emphasis>
Drawings of
<taxonomicName id="4C3E2CF5DC5C831FFEC640A4F3ECFBB0" authorityName="Li &amp; Zhuang &amp; Feng &amp; Al-Farraj &amp; Schrecengost &amp; Roưerova &amp; Beinart &amp; Hu" authorityYear="2023" box="[332,496,1017,1041]" class="Kinetofragminophora" family="Trimyemidae" genus="Trimyema" kingdom="Chromista" order="Trichostomatida" pageId="4" pageNumber="497" phylum="Ciliophora" rank="species" species="foissneri">
<emphasis id="B94A8B64DC5C831FFEC640A4F3ECFBB0" box="[332,496,1017,1041]" italics="true" pageId="4" pageNumber="497">Trimyema foissneri</emphasis>
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from life (AD) and aħer protargol staining (EI). A, leħ lateral view of a representative individual, arrow shows acontractile vacuole, red arrowhead shows food vacuole, yellow arrowheads show somatic cilia.B, different body shape in different viewing angles. C, leħ lateral view, showing buccal cavity (arrow) and position of macronucleus.D, disposition of the ciliary girdles. E, oral ciliature, red dots showing kinetidal composition at the anterior part of OK1. F, lateral view of oral ciliature, showing the shorter oral kinety 3 (in red). G, macronucleus and micronucleus (arrowheads), the position of the laưer being variable (1, 2). H, I, ventral (H) and dorsal (I) views of the holotype to show somatic and oral kineties, arrows show argentophilic lines connecting longitudinal somatic ciliary rows, arrowheads show epaulet. Abbreviations: CC, caudal cilia; Ma, macronucleus; OK, oral kinety; SK, somatic kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (A, C); 10 μm (H, I).
</paragraph>
</caption>
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<emphasis id="B94A8B64DC5C831FFF0B4555F2ECF981" box="[129,240,1544,1568]" italics="true" pageId="4" pageNumber="497">Etymology:</emphasis>
We dedicate this new species to Prof. Wilhelm Foissner, University of
<collectingRegion id="49FA9994DC5C831FFEFE4575F3D3F9E1" box="[372,463,1576,1600]" country="Austria" name="Salzburg" pageId="4" pageNumber="497">Salzburg</collectingRegion>
,
<collectingCountry id="F32917E6DC5C831FFE514575F03BF9E1" box="[475,551,1576,1600]" name="Austria" pageId="4" pageNumber="497">Austria</collectingCountry>
, in recognition of his significant contribution to the taxonomy of ciliates.
</paragraph>
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<materialsCitation id="3B565D2BDC5C831FFF0B45DAF070F91C" country="China" county="Tangdao Bay" latitude="35.931614" location="Sediments" longLatPrecision="1" longitude="120.20252" municipality="Tangdao Bay Park" pageId="4" pageNumber="497" specimenCount="1" typeStatus="holotype">
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locality:
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of intertidal zone in
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</collectingMunicipality>
, ºingdao,
<collectingCountry id="F32917E6DC5C831FFF6E45FBF33FF91F" box="[228,291,1702,1726]" name="China" pageId="4" pageNumber="497">China</collectingCountry>
(
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;
<geoCoordinate id="EE0A31B1DC5C831FFE4645FBF079F91C" box="[460,613,1700,1726]" degrees="120" direction="east" minutes="12" orientation="longitude" pageId="4" pageNumber="497" precision="1" seconds="09.1" value="120.20252">120°12ʹ09.1″E</geoCoordinate>
)
</materialsCitation>
.
</paragraph>
<paragraph id="8B815776DC5C831FFF0B45BBF3A9F818" blockId="4.[129,778,1765,1977]" pageId="4" pageNumber="497">
<emphasis id="B94A8B64DC5C831FFF0B45BBF334F95C" box="[129,296,1765,1789]" italics="true" pageId="4" pageNumber="497">Type deposition:</emphasis>
<materialsCitation id="3B565D2BDC5C831FFECF45B8F3ADF818" country="China" location="Laboratory of Protozoology" pageId="4" pageNumber="497" specimenCount="4" typeStatus="holotype">
The protargol slide (registration number: LR2020062501-01) with the
<typeStatus id="5485E9D4DC5C831FFE234459F019F8BD" box="[425,517,1796,1820]" pageId="4" pageNumber="497">holotype</typeStatus>
specimen (
<figureCitation id="13054BF3DC5C831FFDF34459F0C6F8BD" box="[633,730,1796,1820]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="4" pageNumber="497">Figs 2H, I</figureCitation>
,
<figureCitation id="13054BF3DC5C831FFD694459F0E3F8BD" box="[739,767,1796,1820]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="4" pageNumber="497">3P</figureCitation>
) and several
<typeStatus id="5485E9D4DC5C831FFF704479F34FF89D" box="[250,339,1828,1852]" pageId="4" pageNumber="497">paratype</typeStatus>
specimens, and another slide (registration number: LR2020062501-02) with
<typeStatus id="5485E9D4DC5C831FFE6F441EF022F8FA" box="[485,574,1859,1883]" pageId="4" pageNumber="497">paratype</typeStatus>
specimens were deposited in the
<location id="8EE101ADDC5C831FFE99443FF02EF8DB" LSID="urn:lsid:plazi:treatment:0397E660DC5B831DFC6D45CBF118FCBA:8EE101ADDC5C831FFE99443FF02EF8DB" box="[275,562,1890,1914]" country="China" name="Laboratory of Protozoology" pageId="4" pageNumber="497">Laboratory of Protozoology</location>
, Ocean University of
<collectingCountry id="F32917E6DC5C831FFF0B44DFF2DEF83B" box="[129,194,1922,1946]" name="China" pageId="4" pageNumber="497">China</collectingCountry>
, ºingdao,
<collectingCountry id="F32917E6DC5C831FFEA744DFF372F83B" box="[301,366,1922,1946]" name="China" pageId="4" pageNumber="497">China</collectingCountry>
. The
<typeStatus id="5485E9D4DC5C831FFE2944DFF3E3F83B" box="[419,511,1922,1946]" pageId="4" pageNumber="497">holotype</typeStatus>
was marked by a black ink circle on back side of the slide
</materialsCitation>
.
</paragraph>
<paragraph id="8B815776DC5C831FFCB347ACF650F97F" blockId="4.[825,1475,1264,1978]" pageId="4" pageNumber="497">
<emphasis id="B94A8B64DC5C831FFCB347ACF6A7FAA9" box="[825,1211,1264,1288]" italics="true" pageId="4" pageNumber="497">Description of Chinese population:</emphasis>
Body non-contractile, 2035 × 1020 μm
<emphasis id="B94A8B64DC5C831FFB9E464DF646FA86" box="[1044,1114,1296,1319]" italics="true" pageId="4" pageNumber="497">in vivo</emphasis>
with a ratio of length to width 1.92.4:1 and decreased to 1.21.7:1 aħer protargol staining. Body fusiform with anterior and posterior end slightly narrowed, dorsal side with slight concavity in posterior-third of body (
<figureCitation id="13054BF3DC5C831FFA124630F16BFA04" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="4" pageNumber="497">Figs 2AC</figureCitation>
,
<figureCitation id="13054BF3DC5C831FFC0C46D0F1EAFA05" box="[902,1014,1421,1445]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="4" pageNumber="497">3AC, J, L</figureCitation>
). Macronucleus spherical to broadly ellipsoidal, centrally located or a liưle downward, 58 × 47 µm
<emphasis id="B94A8B64DC5C831FFCB34691F162FA42" box="[825,894,1484,1507]" italics="true" pageId="4" pageNumber="497">in vivo</emphasis>
, many nucleoli always aưached to its outer surface in stained cells, but hardly observed
<emphasis id="B94A8B64DC5C831FFB1C46B1F6CAF9A2" box="[1174,1238,1516,1539]" italics="true" pageId="4" pageNumber="497">in vivo</emphasis>
(
<figureCitation id="13054BF3DC5C831FFB6246B6F768F9A2" box="[1256,1396,1515,1539]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="4" pageNumber="497">Figs 2A, C, G</figureCitation>
,
<figureCitation id="13054BF3DC5C831FFAF546B6F179F983" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="4" pageNumber="497">3C, D, G, P</figureCitation>
). Single globular micronucleus aưached to, or located near, macronucleus, 12 μm across in stained cells, usually unrecognizable
<emphasis id="B94A8B64DC5C831FFC024517F1D4F9C0" box="[904,968,1610,1633]" italics="true" pageId="4" pageNumber="497">in vivo</emphasis>
(
<figureCitation id="13054BF3DC5C831FFC524514F63BF9C0" box="[984,1063,1609,1633]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="4" pageNumber="497">Figs 2G</figureCitation>
,
<figureCitation id="13054BF3DC5C831FFBB94514F64FF9C0" box="[1075,1107,1609,1633]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="4" pageNumber="497">3D</figureCitation>
). Endoplasm colourless, containing numerous 12 μm across lipid droplets, few acontractile vacuoles and food vacuoles located at posterior part of cell (
<figureCitation id="13054BF3DC5C831FFAE445DAF7A7F901" box="[1390,1467,1671,1696]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="4" pageNumber="497">Figs 2A</figureCitation>
,
<figureCitation id="13054BF3DC5C831FFCB345FAF1B3F91F" box="[825,943,1703,1727]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="4" pageNumber="497">3AC, J, M</figureCitation>
). Swims slowly by rotating around main body axis, always spiralling to the leħ.
</paragraph>
<paragraph id="8B815776DC5C831DFCDF45B8F047FEE4" blockId="4.[825,1475,1264,1978]" lastBlockId="6.[128,778,144,795]" lastPageId="6" lastPageNumber="499" pageId="4" pageNumber="497">
Somatic cilia 510 μm long, arranged in 2632 longitudinal somatic ciliary rows, forming three oblique parallel ciliary girdles, visible in both living and stained individuals (
<figureCitation id="13054BF3DC5C831FFAC44479F178F8FA" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="4" pageNumber="497">Figs 2A, D, H, I</figureCitation>
,
<figureCitation id="13054BF3DC5C831FFCFA4419F1CAF8FA" box="[880,982,1860,1884]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="4" pageNumber="497">3FI, L, P</figureCitation>
, º). Girdles beginning right to several kinetids located at the anterior end of oral kineties, extending downward along the cell surface, slightly more than 360°, with the passing surface a liưle dented (
<figureCitation id="13054BF3DC5C831FFBA544FCF69DF818" box="[1071,1153,1953,1977]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="4" pageNumber="497">Figs 2D</figureCitation>
,
<figureCitation id="13054BF3DC5C831FFB0544FCF6AFF818" box="[1167,1203,1953,1977]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="4" pageNumber="497">3M</figureCitation>
). Region below posterior end of last girdle 511 μm long, 36% of body length, with cilia irregularly and loosely arranged, mixing with mucocysts (
<figureCitation id="13054BF3DC5D831EFFF644EFF2FEF86B" box="[124,226,1970,1994]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="5" pageNumber="498">Figs 2H, I</figureCitation>
,
<figureCitation id="13054BF3DC5D831EFF6444EFF31BF86B" box="[238,263,1970,1994]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="5" pageNumber="498">3P</figureCitation>
, º). One flexible caudal cilium, 1118 μm long, about half cell length (
<figureCitation id="13054BF3DC5D831EFB944429F693F82D" box="[1054,1167,1908,1932]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="5" pageNumber="498">Figs 2AC</figureCitation>
,
<figureCitation id="13054BF3DC5D831EFB144429F6C1F82D" box="[1182,1245,1908,1932]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="5" pageNumber="498">3A, C</figureCitation>
). Two to four somatic kineties on right dorsal side of oral area, composed of four to six kinetosomes each, forming a quadrilateral area so-called epaulet (
<figureCitation id="13054BF3DC5E831DFF5443CDF33AFF09" box="[222,294,144,168]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="6" pageNumber="499">Figs 2I</figureCitation>
,
<figureCitation id="13054BF3DC5E831DFEB943CDF376FF09" box="[307,362,144,168]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">3GI</figureCitation>
). Ventrolateral fragment (VLF) located at right ventral side, about the same level as posterior end of oral kineties, comprised of three or four kinetosomes, arranged obliquely, further from somatic and oral kineties (
<figureCitation id="13054BF3DC5E831DFDEE43B3F0A9FEA7" box="[612,693,238,262]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="6" pageNumber="499">Figs 2H</figureCitation>
,
<figureCitation id="13054BF3DC5E831DFD4A43B3F0C5FEA7" box="[704,729,238,262]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">3P</figureCitation>
, º). Thin argentophilic lines visible aħer staining, connecting longitudinally somatic ciliary rows (
<figureCitation id="13054BF3DC5E831DFE314270F003FEE5" box="[443,543,301,325]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="6" pageNumber="499">Figs 2H, I</figureCitation>
,
<figureCitation id="13054BF3DC5E831DFDA34270F056FEE4" box="[553,586,301,325]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">3N</figureCitation>
).
</paragraph>
<caption id="DF4107FEDC5D831EFFFB40BDF77DFB55" ID-DOI="http://doi.org/10.5281/zenodo.8426123" ID-Zenodo-Dep="8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="5" pageNumber="498" startId="5.[113,176,992,1016]" targetBox="[146,1426,146,963]" targetPageId="5" targetType="figure">
<paragraph id="8B815776DC5D831EFFFB40BDF77DFB55" blockId="5.[113,1451,992,1269]" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFFFB40BDF2DBFC59" bold="true" box="[113,199,992,1016]" pageId="5" pageNumber="498">Figure 3.</emphasis>
Photomicrographs of
<taxonomicName id="4C3E2CF5DC5D831EFE1940BCF026FC59" authorityName="Li &amp; Zhuang &amp; Feng &amp; Al-Farraj &amp; Schrecengost &amp; Roưerova &amp; Beinart &amp; Hu" authorityYear="2023" box="[403,570,992,1016]" class="Kinetofragminophora" family="Trimyemidae" genus="Trimyema" kingdom="Chromista" order="Trichostomatida" pageId="5" pageNumber="498" phylum="Ciliophora" rank="species" species="foissneri">
<emphasis id="B94A8B64DC5D831EFE1940BCF026FC59" box="[403,570,992,1016]" italics="true" pageId="5" pageNumber="498">Trimyema foissneri</emphasis>
</taxonomicName>
from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape. C, right lateral views of a slightly compressed individual, arrowhead marks caudal cilia. D, macronucleus and micronucleus (arrowhead). E, cytopharynx fibres (arrowhead). F, lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead). GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads). J, ventral view, arrowhead indicates oval buccal cavity. K, anterior region, showing buccal cavity (arrowhead). L, lateral view of cell, showing three oblique somatic ciliary girdles. M, anterior part of cell, arrowheads show dents on surface. N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P, º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (red circle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts. Abbreviations: G1, first ciliary girdle; G2, second ciliary girdle; G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment. Scale bars: 15 μm (AC); 10 μm (G, P, º).
</paragraph>
</caption>
<caption id="DF4107FEDC5D831EFFFB4645F1E2FA91" box="[113,1022,1304,1328]" pageId="5" pageNumber="498" startId="5.[113,168,1304,1328]" targetType="table">
<paragraph id="8B815776DC5D831EFFFB4645F1E2FA91" blockId="5.[113,1022,1304,1328]" box="[113,1022,1304,1328]" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFFFB4645F2A3FA91" bold="true" box="[113,191,1304,1328]" pageId="5" pageNumber="498">Table 2.</emphasis>
Morphometric characteristics of
<taxonomicName id="4C3E2CF5DC5D831EFE704645F0E0FA91" authority=", Chinese" authorityName="Chinese" box="[506,764,1304,1328]" class="Kinetofragminophora" family="Trimyemidae" genus="Trimyema" kingdom="Chromista" order="Trichostomatida" pageId="5" pageNumber="498" phylum="Ciliophora" rank="species" species="foissneri">
<emphasis id="B94A8B64DC5D831EFE704645F0B8FA91" box="[506,676,1304,1328]" italics="true" pageId="5" pageNumber="498">Trimyema foissneri</emphasis>
, Chinese
</taxonomicName>
population (TANGDAO).
</paragraph>
</caption>
<paragraph id="8B815776DC5D831EFFFB461BF7AFF8A2" pageId="5" pageNumber="498">
<table id="F93EA5D6DC5D7CE4FFFB461BF7AFF8A2" box="[113,1459,1350,1795]" colsContinueIn="9.[113,1459,217,1541]" gridcols="8" gridrows="13" pageId="5" pageNumber="498">
<tr id="350E5534DC5D7CE4FFFB461BF7AFFAFF" box="[113,1459,1350,1374]" gridrow="0" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB461BF066FAFF" box="[113,634,1350,1374]" gridcol="0" gridrow="0" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFFFB461BF2E1FAFF" bold="true" box="[113,253,1350,1374]" pageId="5" pageNumber="498">Characteristic</emphasis>
</th>
<th id="76DF3C48DC5D7CE4FD4D461BF0EDFAFF" box="[711,753,1350,1374]" gridcol="1" gridrow="0" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFD4D461BF0ECFAFF" bold="true" box="[711,752,1350,1374]" pageId="5" pageNumber="498">Min</emphasis>
</th>
<th id="76DF3C48DC5D7CE4FCB5461BF177FAFF" box="[831,875,1350,1374]" gridcol="2" gridrow="0" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFCB5461BF177FAFC" bold="true" box="[831,875,1350,1373]" pageId="5" pageNumber="498">Max</emphasis>
</th>
<th id="76DF3C48DC5D7CE4FC30461BF1EEFAFF" box="[954,1010,1350,1374]" gridcol="3" gridrow="0" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFC30461BF1EEFAFC" bold="true" box="[954,1010,1350,1373]" pageId="5" pageNumber="498">Mean</emphasis>
</th>
<th id="76DF3C48DC5D7CE4FBCA461BF676FAFF" box="[1088,1130,1350,1374]" gridcol="4" gridrow="0" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFBCA461BF649FAFC" bold="true" box="[1088,1109,1350,1373]" pageId="5" pageNumber="498">M</emphasis>
</th>
<th id="76DF3C48DC5D7CE4FB3D461BF6CAFAFF" box="[1207,1238,1350,1374]" gridcol="5" gridrow="0" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFB3D461BF6CAFAFF" bold="true" box="[1207,1238,1350,1374]" pageId="5" pageNumber="498">SD</emphasis>
</th>
<th id="76DF3C48DC5D7CE4FAAE461BF752FAFF" box="[1316,1358,1350,1374]" gridcol="6" gridrow="0" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFAAE461BF759FAFF" bold="true" box="[1316,1349,1350,1374]" pageId="5" pageNumber="498">CV</emphasis>
</th>
<th id="76DF3C48DC5D7CE4FA11461BF7AFFAFF" box="[1435,1459,1350,1374]" gridcol="7" gridrow="0" pageId="5" pageNumber="498">
<emphasis id="B94A8B64DC5D831EFA11461AF7B1FAFF" bold="true" box="[1435,1453,1351,1374]" italics="true" pageId="5" pageNumber="498">N</emphasis>
</th>
</tr>
<tr id="350E5534DC5D7CE4FFFB462BF7AFFA2F" box="[113,1459,1398,1422]" gridrow="1" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB462BF066FA2F" box="[113,634,1398,1422]" gridcol="0" gridrow="1" pageId="5" pageNumber="498">Body, length</th>
<td id="76DF3C48DC5D7CE4FD4D462BF0EDFA2F" box="[711,753,1398,1422]" gridcol="1" gridrow="1" pageId="5" pageNumber="498">18.0</td>
<td id="76DF3C48DC5D7CE4FCB5462BF177FA2F" box="[831,875,1398,1422]" gridcol="2" gridrow="1" pageId="5" pageNumber="498">28.0</td>
<td id="76DF3C48DC5D7CE4FC30462BF1EEFA2F" box="[954,1010,1398,1422]" gridcol="3" gridrow="1" pageId="5" pageNumber="498">22.6</td>
<td id="76DF3C48DC5D7CE4FBCA462BF676FA2F" box="[1088,1130,1398,1422]" gridcol="4" gridrow="1" pageId="5" pageNumber="498">23.0</td>
<td id="76DF3C48DC5D7CE4FB3D462BF6CAFA2F" box="[1207,1238,1398,1422]" gridcol="5" gridrow="1" pageId="5" pageNumber="498">2.2</td>
<td id="76DF3C48DC5D7CE4FAAE462BF752FA2F" box="[1316,1358,1398,1422]" gridcol="6" gridrow="1" pageId="5" pageNumber="498">9.6</td>
<td id="76DF3C48DC5D7CE4FA11462BF7AFFA2F" box="[1435,1459,1398,1422]" gridcol="7" gridrow="1" pageId="5" pageNumber="498">24</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB46C5F7AFFA11" box="[113,1459,1432,1456]" gridrow="2" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB46C5F066FA11" box="[113,634,1432,1456]" gridcol="0" gridrow="2" pageId="5" pageNumber="498">Body, width</th>
<td id="76DF3C48DC5D7CE4FD4D46C5F0EDFA11" box="[711,753,1432,1456]" gridcol="1" gridrow="2" pageId="5" pageNumber="498">12.0</td>
<td id="76DF3C48DC5D7CE4FCB546C5F177FA11" box="[831,875,1432,1456]" gridcol="2" gridrow="2" pageId="5" pageNumber="498">20.0</td>
<td id="76DF3C48DC5D7CE4FC3046C5F1EEFA11" box="[954,1010,1432,1456]" gridcol="3" gridrow="2" pageId="5" pageNumber="498">15.1</td>
<td id="76DF3C48DC5D7CE4FBCA46C5F676FA11" box="[1088,1130,1432,1456]" gridcol="4" gridrow="2" pageId="5" pageNumber="498">15.0</td>
<td id="76DF3C48DC5D7CE4FB3D46C5F6CAFA11" box="[1207,1238,1432,1456]" gridcol="5" gridrow="2" pageId="5" pageNumber="498">2.2</td>
<td id="76DF3C48DC5D7CE4FAAE46C5F752FA11" box="[1316,1358,1432,1456]" gridcol="6" gridrow="2" pageId="5" pageNumber="498">14.4</td>
<td id="76DF3C48DC5D7CE4FA1146C5F7AFFA11" box="[1435,1459,1432,1456]" gridcol="7" gridrow="2" pageId="5" pageNumber="498">24</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB46E7F7AFFA73" box="[113,1459,1466,1490]" gridrow="3" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB46E7F066FA73" box="[113,634,1466,1490]" gridcol="0" gridrow="3" pageId="5" pageNumber="498">Body length:width, ratio</th>
<td id="76DF3C48DC5D7CE4FD4D46E7F0EDFA73" box="[711,753,1466,1490]" gridcol="1" gridrow="3" pageId="5" pageNumber="498">1.2</td>
<td id="76DF3C48DC5D7CE4FCB546E7F177FA73" box="[831,875,1466,1490]" gridcol="2" gridrow="3" pageId="5" pageNumber="498">1.7</td>
<td id="76DF3C48DC5D7CE4FC3046E7F1EEFA73" box="[954,1010,1466,1490]" gridcol="3" gridrow="3" pageId="5" pageNumber="498">1.5</td>
<td id="76DF3C48DC5D7CE4FBCA46E7F676FA73" box="[1088,1130,1466,1490]" gridcol="4" gridrow="3" pageId="5" pageNumber="498">1.5</td>
<td id="76DF3C48DC5D7CE4FB3D46E7F6CAFA73" box="[1207,1238,1466,1490]" gridcol="5" gridrow="3" pageId="5" pageNumber="498">0.1</td>
<td id="76DF3C48DC5D7CE4FAAE46E7F752FA73" box="[1316,1358,1466,1490]" gridcol="6" gridrow="3" pageId="5" pageNumber="498">9.1</td>
<td id="76DF3C48DC5D7CE4FA1146E7F7AFFA73" box="[1435,1459,1466,1490]" gridcol="7" gridrow="3" pageId="5" pageNumber="498">24</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB4681F7AFFA55" box="[113,1459,1500,1524]" gridrow="4" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB4681F066FA55" box="[113,634,1500,1524]" gridcol="0" gridrow="4" pageId="5" pageNumber="498">Macronucleus, length</th>
<td id="76DF3C48DC5D7CE4FD4D4681F0EDFA55" box="[711,753,1500,1524]" gridcol="1" gridrow="4" pageId="5" pageNumber="498">6.0</td>
<td id="76DF3C48DC5D7CE4FCB54681F177FA55" box="[831,875,1500,1524]" gridcol="2" gridrow="4" pageId="5" pageNumber="498">10.0</td>
<td id="76DF3C48DC5D7CE4FC304681F1EEFA55" box="[954,1010,1500,1524]" gridcol="3" gridrow="4" pageId="5" pageNumber="498">7.4</td>
<td id="76DF3C48DC5D7CE4FBCA4681F676FA55" box="[1088,1130,1500,1524]" gridcol="4" gridrow="4" pageId="5" pageNumber="498">7.0</td>
<td id="76DF3C48DC5D7CE4FB3D4681F6CAFA55" box="[1207,1238,1500,1524]" gridcol="5" gridrow="4" pageId="5" pageNumber="498">1.2</td>
<td id="76DF3C48DC5D7CE4FAAE4681F752FA55" box="[1316,1358,1500,1524]" gridcol="6" gridrow="4" pageId="5" pageNumber="498">17.2</td>
<td id="76DF3C48DC5D7CE4FA114681F7AFFA55" box="[1435,1459,1500,1524]" gridcol="7" gridrow="4" pageId="5" pageNumber="498">23</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB46A0F7AFF9B7" box="[113,1459,1533,1558]" gridrow="5" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB46A0F066F9B7" box="[113,634,1533,1558]" gridcol="0" gridrow="5" pageId="5" pageNumber="498">Macronucleus, width</th>
<td id="76DF3C48DC5D7CE4FD4D46A0F0EDF9B7" box="[711,753,1533,1558]" gridcol="1" gridrow="5" pageId="5" pageNumber="498">4.0</td>
<td id="76DF3C48DC5D7CE4FCB546A0F177F9B7" box="[831,875,1533,1558]" gridcol="2" gridrow="5" pageId="5" pageNumber="498">8.0</td>
<td id="76DF3C48DC5D7CE4FC3046A0F1EEF9B7" box="[954,1010,1533,1558]" gridcol="3" gridrow="5" pageId="5" pageNumber="498">6.0</td>
<td id="76DF3C48DC5D7CE4FBCA46A0F676F9B7" box="[1088,1130,1533,1558]" gridcol="4" gridrow="5" pageId="5" pageNumber="498">6.0</td>
<td id="76DF3C48DC5D7CE4FB3D46A0F6CAF9B7" box="[1207,1238,1533,1558]" gridcol="5" gridrow="5" pageId="5" pageNumber="498">1.0</td>
<td id="76DF3C48DC5D7CE4FAAE46A0F752F9B7" box="[1316,1358,1533,1558]" gridcol="6" gridrow="5" pageId="5" pageNumber="498">17.4</td>
<td id="76DF3C48DC5D7CE4FA1146A0F7AFF9B7" box="[1435,1459,1533,1558]" gridcol="7" gridrow="5" pageId="5" pageNumber="498">23</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB4542F7AFF999" box="[113,1459,1567,1592]" gridrow="6" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB4542F066F999" box="[113,634,1567,1592]" gridcol="0" gridrow="6" pageId="5" pageNumber="498">Micronucleus, diameter</th>
<td id="76DF3C48DC5D7CE4FD4D4542F0EDF999" box="[711,753,1567,1592]" gridcol="1" gridrow="6" pageId="5" pageNumber="498">1.0</td>
<td id="76DF3C48DC5D7CE4FCB54542F177F999" box="[831,875,1567,1592]" gridcol="2" gridrow="6" pageId="5" pageNumber="498">2.0</td>
<td id="76DF3C48DC5D7CE4FC304542F1EEF999" box="[954,1010,1567,1592]" gridcol="3" gridrow="6" pageId="5" pageNumber="498">1.5</td>
<td id="76DF3C48DC5D7CE4FBCA4542F676F999" box="[1088,1130,1567,1592]" gridcol="4" gridrow="6" pageId="5" pageNumber="498">1.5</td>
<td id="76DF3C48DC5D7CE4FB3D4542F6CAF999" box="[1207,1238,1567,1592]" gridcol="5" gridrow="6" pageId="5" pageNumber="498">0.5</td>
<td id="76DF3C48DC5D7CE4FAAE4542F752F999" box="[1316,1358,1567,1592]" gridcol="6" gridrow="6" pageId="5" pageNumber="498">33.3</td>
<td id="76DF3C48DC5D7CE4FA114542F7AFF999" box="[1435,1459,1567,1592]" gridcol="7" gridrow="6" pageId="5" pageNumber="498">16</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB451CF7AFF9F8" box="[113,1459,1601,1625]" gridrow="7" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB451CF066F9F8" box="[113,634,1601,1625]" gridcol="0" gridrow="7" pageId="5" pageNumber="498">Oral field, length</th>
<td id="76DF3C48DC5D7CE4FD4D451CF0EDF9F8" box="[711,753,1601,1625]" gridcol="1" gridrow="7" pageId="5" pageNumber="498">4.0</td>
<td id="76DF3C48DC5D7CE4FCB5451CF177F9F8" box="[831,875,1601,1625]" gridcol="2" gridrow="7" pageId="5" pageNumber="498">8.0</td>
<td id="76DF3C48DC5D7CE4FC30451CF1EEF9F8" box="[954,1010,1601,1625]" gridcol="3" gridrow="7" pageId="5" pageNumber="498">6.6</td>
<td id="76DF3C48DC5D7CE4FBCA451CF676F9F8" box="[1088,1130,1601,1625]" gridcol="4" gridrow="7" pageId="5" pageNumber="498">7.0</td>
<td id="76DF3C48DC5D7CE4FB3D451CF6CAF9F8" box="[1207,1238,1601,1625]" gridcol="5" gridrow="7" pageId="5" pageNumber="498">1.0</td>
<td id="76DF3C48DC5D7CE4FAAE451CF752F9F8" box="[1316,1358,1601,1625]" gridcol="6" gridrow="7" pageId="5" pageNumber="498">14.8</td>
<td id="76DF3C48DC5D7CE4FA11451CF7AFF9F8" box="[1435,1459,1601,1625]" gridcol="7" gridrow="7" pageId="5" pageNumber="498">24</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB453EF7AFF9DA" box="[113,1459,1635,1659]" gridrow="8" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB453EF066F9DA" box="[113,634,1635,1659]" gridcol="0" gridrow="8" pageId="5" pageNumber="498">Cytopharyngeal basket, length</th>
<td id="76DF3C48DC5D7CE4FD4D453EF0EDF9DA" box="[711,753,1635,1659]" gridcol="1" gridrow="8" pageId="5" pageNumber="498">11.0</td>
<td id="76DF3C48DC5D7CE4FCB5453EF177F9DA" box="[831,875,1635,1659]" gridcol="2" gridrow="8" pageId="5" pageNumber="498">17.0</td>
<td id="76DF3C48DC5D7CE4FC30453EF1EEF9DA" box="[954,1010,1635,1659]" gridcol="3" gridrow="8" pageId="5" pageNumber="498">13.9</td>
<td id="76DF3C48DC5D7CE4FBCA453EF676F9DA" box="[1088,1130,1635,1659]" gridcol="4" gridrow="8" pageId="5" pageNumber="498">14.0</td>
<td id="76DF3C48DC5D7CE4FB3D453EF6CAF9DA" box="[1207,1238,1635,1659]" gridcol="5" gridrow="8" pageId="5" pageNumber="498">1.5</td>
<td id="76DF3C48DC5D7CE4FAAE453EF752F9DA" box="[1316,1358,1635,1659]" gridcol="6" gridrow="8" pageId="5" pageNumber="498">10.4</td>
<td id="76DF3C48DC5D7CE4FA11453EF7AFF9DA" box="[1435,1459,1635,1659]" gridcol="7" gridrow="8" pageId="5" pageNumber="498">23</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB45D8F7AFF93C" box="[113,1459,1669,1693]" gridrow="9" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB45D8F066F93C" box="[113,634,1669,1693]" gridcol="0" gridrow="9" pageId="5" pageNumber="498">End of last ciliary girdle to posterior body end, distance</th>
<td id="76DF3C48DC5D7CE4FD4D45D8F0EDF93C" box="[711,753,1669,1693]" gridcol="1" gridrow="9" pageId="5" pageNumber="498">5.0</td>
<td id="76DF3C48DC5D7CE4FCB545D8F177F93C" box="[831,875,1669,1693]" gridcol="2" gridrow="9" pageId="5" pageNumber="498">11.0</td>
<td id="76DF3C48DC5D7CE4FC3045D8F1EEF93C" box="[954,1010,1669,1693]" gridcol="3" gridrow="9" pageId="5" pageNumber="498">8.1</td>
<td id="76DF3C48DC5D7CE4FBCA45D8F676F93C" box="[1088,1130,1669,1693]" gridcol="4" gridrow="9" pageId="5" pageNumber="498">8.0</td>
<td id="76DF3C48DC5D7CE4FB3D45D8F6CAF93C" box="[1207,1238,1669,1693]" gridcol="5" gridrow="9" pageId="5" pageNumber="498">1.5</td>
<td id="76DF3C48DC5D7CE4FAAE45D8F752F93C" box="[1316,1358,1669,1693]" gridcol="6" gridrow="9" pageId="5" pageNumber="498">19.2</td>
<td id="76DF3C48DC5D7CE4FA1145D8F7AFF93C" box="[1435,1459,1669,1693]" gridcol="7" gridrow="9" pageId="5" pageNumber="498">24</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB45FAF7AFF91E" box="[113,1459,1703,1727]" gridrow="10" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB45FAF066F91E" box="[113,634,1703,1727]" gridcol="0" gridrow="10" pageId="5" pageNumber="498">Anterior body end to macronucleus, distance</th>
<td id="76DF3C48DC5D7CE4FD4D45FAF0EDF91E" box="[711,753,1703,1727]" gridcol="1" gridrow="10" pageId="5" pageNumber="498">4.0</td>
<td id="76DF3C48DC5D7CE4FCB545FAF177F91E" box="[831,875,1703,1727]" gridcol="2" gridrow="10" pageId="5" pageNumber="498">15.0</td>
<td id="76DF3C48DC5D7CE4FC3045FAF1EEF91E" box="[954,1010,1703,1727]" gridcol="3" gridrow="10" pageId="5" pageNumber="498">8.8</td>
<td id="76DF3C48DC5D7CE4FBCA45FAF676F91E" box="[1088,1130,1703,1727]" gridcol="4" gridrow="10" pageId="5" pageNumber="498">9.0</td>
<td id="76DF3C48DC5D7CE4FB3D45FAF6CAF91E" box="[1207,1238,1703,1727]" gridcol="5" gridrow="10" pageId="5" pageNumber="498">2.3</td>
<td id="76DF3C48DC5D7CE4FAAE45FAF752F91E" box="[1316,1358,1703,1727]" gridcol="6" gridrow="10" pageId="5" pageNumber="498">25.5</td>
<td id="76DF3C48DC5D7CE4FA1145FAF7AFF91E" box="[1435,1459,1703,1727]" gridcol="7" gridrow="10" pageId="5" pageNumber="498">24</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB4595F7AFF940" box="[113,1459,1736,1761]" gridrow="11" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB4595F066F940" box="[113,634,1736,1761]" gridcol="0" gridrow="11" pageId="5" pageNumber="498">Longitudinal somatic ciliary rows, number</th>
<td id="76DF3C48DC5D7CE4FD4D4595F0EDF940" box="[711,753,1736,1761]" gridcol="1" gridrow="11" pageId="5" pageNumber="498">26.0</td>
<td id="76DF3C48DC5D7CE4FCB54595F177F940" box="[831,875,1736,1761]" gridcol="2" gridrow="11" pageId="5" pageNumber="498">32.0</td>
<td id="76DF3C48DC5D7CE4FC304595F1EEF940" box="[954,1010,1736,1761]" gridcol="3" gridrow="11" pageId="5" pageNumber="498">29.0</td>
<td id="76DF3C48DC5D7CE4FBCA4595F676F940" box="[1088,1130,1736,1761]" gridcol="4" gridrow="11" pageId="5" pageNumber="498">29.0</td>
<td id="76DF3C48DC5D7CE4FB3D4595F6CAF940" box="[1207,1238,1736,1761]" gridcol="5" gridrow="11" pageId="5" pageNumber="498">1.6</td>
<td id="76DF3C48DC5D7CE4FAAE4595F752F940" box="[1316,1358,1736,1761]" gridcol="6" gridrow="11" pageId="5" pageNumber="498">5.3</td>
<td id="76DF3C48DC5D7CE4FA114595F7AFF940" box="[1435,1459,1736,1761]" gridcol="7" gridrow="11" pageId="5" pageNumber="498">24</td>
</tr>
<tr id="350E5534DC5D7CE4FFFB45B7F7AFF8A2" box="[113,1459,1770,1795]" gridrow="12" pageId="5" pageNumber="498">
<th id="76DF3C48DC5D7CE4FFFB45B7F066F8A2" box="[113,634,1770,1795]" gridcol="0" gridrow="12" pageId="5" pageNumber="498">Somatic ciliary girdles, number</th>
<td id="76DF3C48DC5D7CE4FD4D45B7F0EDF8A2" box="[711,753,1770,1795]" gridcol="1" gridrow="12" pageId="5" pageNumber="498">3.0</td>
<td id="76DF3C48DC5D7CE4FCB545B7F177F8A2" box="[831,875,1770,1795]" gridcol="2" gridrow="12" pageId="5" pageNumber="498">3.0</td>
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<td id="76DF3C48DC5D7CE4FA1145B7F7AFF8A2" box="[1435,1459,1770,1795]" gridcol="7" gridrow="12" pageId="5" pageNumber="498">24</td>
</tr>
</table>
</paragraph>
<paragraph id="8B815776DC5D831EFFFB4442F0ACF8E9" blockId="5.[113,1401,1823,1864]" pageId="5" pageNumber="498">
<tableNote id="76D856F8DC5D831EFFFB4442F0ACF8E9" pageId="5" pageNumber="498" targetBox="[113,1459,1350,1795]" targetPageId="5">
All data are based on protargol-stained specimens.Measurements in μm.Abbreviations:CV,coefficient of variation in %; M, median; Max, maximum;Min, minimum;Mean, arithmetic mean;
<emphasis id="B94A8B64DC5D831EFF7F4468F31FF8E6" box="[245,259,1845,1863]" italics="true" pageId="5" pageNumber="498">N</emphasis>
, number of specimens examined;SD, standard deviation.
</tableNote>
</paragraph>
<paragraph id="8B815776DC5E831DFF164211F118FCBA" blockId="6.[128,778,144,795]" pageId="6" pageNumber="499">
Oral region oval in shape, 48 μm long, about 30% of cell length (
<figureCitation id="13054BF3DC5E831DFF5E4236F358FE22" box="[212,324,363,387]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="6" pageNumber="499">Figs 2AC</figureCitation>
,
<figureCitation id="13054BF3DC5E831DFED84236F37BFE22" box="[338,359,363,387]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">3J</figureCitation>
). Funnel-like buccal cavity, transversely extending from lower half of oral region to dorsal side (
<figureCitation id="13054BF3DC5E831DFD3F42D6F118FE02" box="[693,772,395,419]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="6" pageNumber="499">Figs 2C</figureCitation>
,
<figureCitation id="13054BF3DC5E831DFF0B42F7F2BCFE63" box="[129,160,426,450]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">3K</figureCitation>
). Oral ciliature including three monokinetidal oral kineties on leħ margin of oral region. Two longer oral kineties in a semicircle. Oral kinety 2 (OK2) slightly shorter than oral kinety 1 (OK1). OK1 and OK2 crossed at about midportion or slightly below (
<figureCitation id="13054BF3DC5E831DFF58417AF37DFD9E" box="[210,353,551,575]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="6" pageNumber="499">Figs 2E, F, H</figureCitation>
,
<figureCitation id="13054BF3DC5E831DFEFA417AF3AEFD9E" box="[368,434,551,575]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">3F, O</figureCitation>
–º). Oral kinety 3 (OK3) especially short, hard to distinguish on ventral side, but sometimes recognized in leħ lateral view (
<figureCitation id="13054BF3DC5E831DFE5F413BF03FFDDF" box="[469,547,614,638]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="6" pageNumber="499">Figs 2F</figureCitation>
,
<figureCitation id="13054BF3DC5E831DFDBE413BF053FDDF" box="[564,591,614,638]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">3F</figureCitation>
). Kinetidal composition at the anterior part of OK1 is highly variable, usually formed by three dikinetids but a few individuals appear in other combinations such as two dikinetids or two dikinetids with one trikinetid (
<figureCitation id="13054BF3DC5E831DFEAD41BEF380FD5A" box="[295,412,739,763]" captionStart="Figure2" captionStartId="4.[129,192,1016,1040]" captionTargetBox="[161,1418,144,989]" captionTargetId="figure-446@4.[161,1441,144,989]" captionTargetPageId="4" captionText="Figure2. Drawings of Trimyema foissneri from life (AD) and aħer protargol staining (EI).A, leħ lateral view of a representative individual,arrow shows acontractile vacuole,red arrowhead shows food vacuole, yellow arrowheads showsomatic cilia.B,different body shapein different viewing angles.C, leħ lateral view, showingbuccal cavity (arrow)and position of macronucleus.D, disposition of the ciliary girdles.E, oral ciliature,red dots showingkinetidal composition at the anterior part ofOK1.F,lateral view of oral ciliature,showing theshorter oral kinety 3 (inred).G, macronucleus andmicronucleus (arrowheads),the position of the laưer being variable (1, 2). H, I, ventral(H) anddorsal (I) views of the holotype to show somatic andoral kineties, arrowsshow argentophilic lines connecting longitudinal somaticciliary rows, arrowheads showepaulet.Abbreviations:CC, caudal cilia;Ma, macronucleus;OK, oral kinety; SK, somatickineties; VLF,ventrolateral fragment.Scale bars:15μm (A, C);10 μm (H, I)." figureDoi="http://doi.org/10.5281/zenodo.8426121" httpUri="https://zenodo.org/record/8426121/files/figure.png" pageId="6" pageNumber="499">Figs 2E, H</figureCitation>
,
<figureCitation id="13054BF3DC5E831DFE2641BEF3D1FD5A" box="[428,461,739,763]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">3O</figureCitation>
). Cytopharyngeal fibres thin, S-shaped, only visible in stained cells, 1117 μm long (
<figureCitation id="13054BF3DC5E831DFD27405FF0EFFCBA" box="[685,755,770,795]" captionStart="Figure 3" captionStartId="5.[113,176,992,1016]" captionTargetBox="[146,1426,146,963]" captionTargetId="figure-290@5.[145,1428,144,964]" captionTargetPageId="5" captionText="Figure 3. Photomicrographs of Trimyema foissneri from life (AC, JM) and aħer protargol staining (DI, Nº). A, B, right lateral views of different individuals, showing different body shape.C, right lateral views of a slightlycompressed individual, arrowhead marks caudal cilia.D, macronucleus and micronucleus (arrowhead).E, cytopharynx fibres (arrowhead).F,lateral view of anterior part of cell, showing two longer oral kineties and a shorter one (arrowhead).GI, right lateral (G, I) and dorsal (H) view, showing epaulet (arrowheads).J,ventral view,arrowhead indicates oval buccal cavity.K, anterior region, showing buccal cavity (arrowhead).L, lateral view of cell, showing three oblique somatic ciliary girdles.M, anterior part of cell, arrowheads show dents on surface.N, argentophilic lines (arrowhead) connected longitudinal somatic ciliary rows. O, details of oral region, arrowheads indicate kinetids at anterior part of oral kinety 1. P,º, ventral view of the holotype (P) and a paratype (º), showing somatic kineties, oral kineties and different ventro-lateral fragment (redcircle), black arrowhead shows first oral kineties, arrow shows second oral kineties, red arrowheads show mucocysts.Abbreviations:G1, first ciliary girdle; G2, second ciliary girdle;G3, third ciliary girdle; Ma, macronucleus; OK1, first oral kineties; OK2, second oral kineties; VLF, ventrolateral fragment.Scale bars: 15 μm (AC); 10 μm (G, P, º)." figureDoi="http://doi.org/10.5281/zenodo.8426123" httpUri="https://zenodo.org/record/8426123/files/figure.png" pageId="6" pageNumber="499">Fig. 3E</figureCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>