126 lines
15 KiB
XML
126 lines
15 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/zookeys.1015.54387" ID-GBIF-Dataset="7f0a1826-ba89-4ecb-adbb-275211293c87" ID-PMC="PMC7878468" ID-Pensoft-Pub="1313-2970-1015-1" ID-Pensoft-UUID="AF1641758561525C8D40BBF3F895FA8A" ID-PubMed="33613041" ID-ZooBank="F6BD92139DB74564AA003C61B2F43B2D" ModsDocID="1313-2970-1015-1" checkinTime="1612497320802" checkinUser="pensoft" docAuthor="Abe, Hirokazu & Sato-Okoshi, Waka" docDate="2021" docId="47575CD0108E517296BEA0A2071A912C" docLanguage="en" docName="ZooKeys 1015: 1-86" docOrigin="ZooKeys 1015" docPubDate="2021-02-04" docSource="http://dx.doi.org/10.3897/zookeys.1015.54387" docTitle="Spiophanes uschakowi Zachs 1933" docType="treatment" docVersion="5" id="AF1641758561525C8D40BBF3F895FA8A" lastPageNumber="1" masterDocId="AF1641758561525C8D40BBF3F895FA8A" masterDocTitle="Molecular identification and larval morphology of spionid polychaetes (Annelida, Spionidae) from northeastern Japan" masterLastPageNumber="86" masterPageNumber="1" pageNumber="1" updateTime="1668149773418" updateUser="ExternalLinkService">
|
|
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
|
|
<mods:titleInfo>
|
|
<mods:title>Molecular identification and larval morphology of spionid polychaetes (Annelida, Spionidae) from northeastern Japan</mods:title>
|
|
</mods:titleInfo>
|
|
<mods:name type="personal">
|
|
<mods:role>
|
|
<mods:roleTerm>Author</mods:roleTerm>
|
|
</mods:role>
|
|
<mods:namePart>Abe, Hirokazu</mods:namePart>
|
|
<mods:nameIdentifier type="ORCID">https://orcid.org/0000-0002-7753-9368</mods:nameIdentifier>
|
|
<mods:affiliation>Department of Biology, Center for Liberal Arts & Sciences, Iwate Medical University, Idaidori 1 - 1 - 1, Yahaba-cho, Shiwa-gun, Iwate 028 - 3694, Japan</mods:affiliation>
|
|
<mods:nameIdentifier type="email">habe@iwate-med.ac.jp</mods:nameIdentifier>
|
|
</mods:name>
|
|
<mods:name type="personal">
|
|
<mods:role>
|
|
<mods:roleTerm>Author</mods:roleTerm>
|
|
</mods:role>
|
|
<mods:namePart>Sato-Okoshi, Waka</mods:namePart>
|
|
<mods:affiliation>Laboratory of Biological Oceanography, Graduate School of Agricultural Science, Tohoku University, Aramaki-Aza-Aoba 468 - 1, Aoba-ku, Sendai 980 - 8572, Japan</mods:affiliation>
|
|
</mods:name>
|
|
<mods:typeOfResource>text</mods:typeOfResource>
|
|
<mods:relatedItem type="host">
|
|
<mods:titleInfo>
|
|
<mods:title>ZooKeys</mods:title>
|
|
</mods:titleInfo>
|
|
<mods:part>
|
|
<mods:date>2021</mods:date>
|
|
<mods:detail type="pubDate">
|
|
<mods:number>2021-02-04</mods:number>
|
|
</mods:detail>
|
|
<mods:detail type="volume">
|
|
<mods:number>1015</mods:number>
|
|
</mods:detail>
|
|
<mods:extent unit="page">
|
|
<mods:start>1</mods:start>
|
|
<mods:end>86</mods:end>
|
|
</mods:extent>
|
|
</mods:part>
|
|
</mods:relatedItem>
|
|
<mods:location>
|
|
<mods:url>http://dx.doi.org/10.3897/zookeys.1015.54387</mods:url>
|
|
</mods:location>
|
|
<mods:classification>journal article</mods:classification>
|
|
<mods:identifier type="DOI">http://dx.doi.org/10.3897/zookeys.1015.54387</mods:identifier>
|
|
<mods:identifier type="Pensoft-Pub">1313-2970-1015-1</mods:identifier>
|
|
<mods:identifier type="ZooBank">F6BD92139DB74564AA003C61B2F43B2D</mods:identifier>
|
|
<mods:identifier type="Pensoft-UUID">AF1641758561525C8D40BBF3F895FA8A</mods:identifier>
|
|
</mods:mods>
|
|
<treatment ID-GBIF-Taxon="177212509" LSID="urn:lsid:plazi:treatment:47575CD0108E517296BEA0A2071A912C" httpUri="http://treatment.plazi.org/id/47575CD0108E517296BEA0A2071A912C" lastPageNumber="1" pageId="0" pageNumber="1">
|
|
<subSubSection pageId="0" pageNumber="1" type="nomenclature">
|
|
<paragraph pageId="0" pageNumber="1">
|
|
<taxonomicName LSID="47575CD0-108E-5172-96BE-A0A2071A912C" authority="Zachs, 1933" authorityName="Zachs" authorityYear="1933" class="Polychaeta" family="Spionidae" genus="Spiophanes" higherTaxonomySource="treatment-meta" kingdom="Animalia" lsidName="Spiophanes uschakowi" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="species" species="uschakowi">Spiophanes aff. uschakowi Zachs, 1933</taxonomicName>
|
|
<figureCitation captionStart="Figure 4" captionStartId="F5" captionText="Figure 4. Light micrographs showing morphologies of living spionid larvae of Aonides, Laonice, Rhynchospio, Scolelepis, and Spiophanes A-C Aonides cf. oxycephala, dorsal view of early planktonic (A), 8 - chaetiger (B), and 18 - chaetiger larvae (C) D Laonice sp. 1, dorsal view of early planktonic larva E Laonice sp. 2, dorsal view of 12 - chaetiger larva F Poecilochaetus sp., dorsal view of 17 - chaetiger larva G, H Rhynchospio aff. asiatica, dorsal view of 6 - chaetiger (G) and 12 - chaetiger larvae (H) I Scolelepis cf. kudenovi, dorsal view of 7 - chaetiger larva J, K Scolelepis sp. 1, dorsal view of 17 - chaetiger (J) and 19 - chaetiger larvae (K) L Scolelepis sp. 2, dorsal view of 20 - chaetiger larva M, N Spiophanes uschakowi, dorsal (M) and lateral view (N) of 18 - chaetiger larvae O, P Spiophanes aff. uschakowi, dorsal view of 16 - chaetiger larva (O) and 27 - chaetiger juvenile (P). Scale bars: 300 μm." figureDoi="10.3897/zookeys.1015.54387.figure4" httpUri="https://binary.pensoft.net/fig/505191" pageId="0" pageNumber="1">Fig. 4O, P</figureCitation>
|
|
</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="larval morphology">
|
|
<paragraph pageId="0" pageNumber="1">Larval morphology.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">Overall shape slender. Prostomium broad and slightly notched anteriorly. In late larvae, a pair of prominent antero-lateral processes on prostomium developed. Two pairs of red or dark red eyes present, lateral ones situated somewhat anteriorly. Lateral parts of peristomium moderately demarcated from prostomium, palps on lateral-most parts of peristomium. Nototrochs occur from chaetiger II onwards. Pharynx and proctodaeum black in color internally. Pygidium acquires dorsal cirri in late larvae. Some brownish, yellowish, or greenish pigmentation occurred on various locations of body in late larvae.</paragraph>
|
|
</subSubSection>
|
|
<subSubSection pageId="0" pageNumber="1" type="remarks">
|
|
<paragraph pageId="0" pageNumber="1">Remarks.</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
Adult individuals of this species were collected from muddy bottom sediments at 22 m depth in Onagawa Bay in April and May 2012 by using a Smith-McIntyre grab sampler and from bottom sediments of the shallow subtidal zone in Sasuhama in February 2012. Adult morphology agrees with the description of
|
|
<taxonomicName lsidName="S. uschakowi" pageId="0" pageNumber="1" rank="species" species="uschakowi">S. cf. uschakowi</taxonomicName>
|
|
by
|
|
<bibRefCitation DOI="https://doi.org/10.11646/zootaxa.2278.1.1" author="Meissner, K" journalOrPublisher="Zootaxa" pageId="0" pageNumber="1" pagination="1 - 25" refId="B112" refString="Meissner, K, Blank, M, 2009. Spiophanes norrisi sp. nov. (Polychaeta: Spionidae) - a new species from the NE Pacific coast, separated from the Spiophanes bombyx complex based on both morphological and genetic studies. Zootaxa 2278: 1 - 25, DOI: https://doi.org/10.11646/zootaxa.2278.1.1" title="Spiophanes norrisi sp. nov. (Polychaeta: Spionidae) - a new species from the NE Pacific coast, separated from the Spiophanes bombyx complex based on both morphological and genetic studies." url="https://doi.org/10.11646/zootaxa.2278.1.1" volume="2278" year="2009">
|
|
<normalizedToken originalValue="Meißner">Meissner</normalizedToken>
|
|
and Blank (2009)
|
|
</bibRefCitation>
|
|
as well as with that of
|
|
<taxonomicName lsidName="S. bombyx" pageId="0" pageNumber="1" rank="species" species="bombyx">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">S. bombyx</emphasis>
|
|
</taxonomicName>
|
|
by
|
|
<bibRefCitation author="Imajima, M" journalOrPublisher="Zoology" pageId="0" pageNumber="1" pagination="115 - 137" refId="B84" refString="Imajima, M, 1991b. Spionidae (Annelida, Polychaeta) from Japan VII. The genus Spiophanes. Bulletin of the National Science Museum, Tokyo, Series A. Zoology 17: 115 - 137" title="Spionidae (Annelida, Polychaeta) from Japan VII. The genus Spiophanes. Bulletin of the National Science Museum, Tokyo, Series A." volume="17" year="1991 b">Imajima (1991b)</bibRefCitation>
|
|
. However, the 18S and 16S rRNA gene sequences of this species did not match those of
|
|
<taxonomicName lsidName="S. uschakowi" pageId="0" pageNumber="1" rank="species" species="uschakowi">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">S. uschakowi</emphasis>
|
|
</taxonomicName>
|
|
obtained from DDBJ/EMBL/GenBank (KM998760): there was a 0.29% (5/1750 bp) and 0.88% (3/342) difference, respectively between these two species. Therefore, this species was referred to
|
|
<taxonomicName lsidName="S. uschakowi" pageId="0" pageNumber="1" rank="species" species="uschakowi">S. aff. uschakowi</taxonomicName>
|
|
. The larvae and adults were confirmed to 100% match using molecular data (Fig.
|
|
<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Maximum Likelihood tree inferred from nuclear 18 S and mitochondrial 16 S rRNA gene sequences of spionids obtained from Japan in the present and previous studies (provided in Table 1). The gene sequences of adult and larval spionid polychaetes are indicated by solid squares and circles in front of each species name, respectively. SH-aLRT / approximate Bayes support / ultrafast bootstrap support values of ≥ 80 %, ≥ 0.95, ≥ 95 %, respectively are given beside the respective nodes. Nodes with red circles indicate triple high support values of SH-aLRT ≥ 80, approximate Bayes support ≥ 0.95, and ultrafast bootstrap support ≥ 95. The scale bar represents the number of substitutions per site. Sequences of Laonome sp. and Sabella pavonina Savigny, 1822 obtained from DDBJ / EMBL / GenBank were used for outgroup rooting." figureDoi="10.3897/zookeys.1015.54387.figure2" httpUri="https://binary.pensoft.net/fig/505188" pageId="0" pageNumber="1">2</figureCitation>
|
|
).
|
|
</paragraph>
|
|
<paragraph pageId="0" pageNumber="1">
|
|
Planktonic larvae of this species were collected in Onagawa Bay in November 2011 and in Sasuhama in February 2012. The larval morphology of
|
|
<taxonomicName lsidName="S. uschakowi" pageId="0" pageNumber="1" rank="species" species="uschakowi">S. aff. uschakowi</taxonomicName>
|
|
was different from that of
|
|
<taxonomicName lsidName="S. uschakowi" pageId="0" pageNumber="1" rank="species" species="uschakowi">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">S. uschakowi</emphasis>
|
|
</taxonomicName>
|
|
in the following aspects: prostomium of the former is broad and slightly notched anteriorly, whereas that of the latter is relatively small and anteriorly rounded; the peristomium of the former is well demarcated from the prostomium, but that of the latter is relatively less demarcated; nototrochs of the former occur from chaetiger II onwards, whereas those of the latter occur from chaetiger IV onwards; pigmentation inside the pharynx is black in the former but brown in the latter; and black pigmentation in the proctodaeum is present in the former but absent in the latter. Black pigmentation in the pharynx and proctodaeum were also reported in the larvae of
|
|
<taxonomicName lsidName="S. bombyx" pageId="0" pageNumber="1" rank="species" species="bombyx">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">S. bombyx</emphasis>
|
|
</taxonomicName>
|
|
(
|
|
<bibRefCitation author="Hannerz, L" journalOrPublisher="Zoologiska bidrag fran Uppsala" pageId="0" pageNumber="1" pagination="1 - 204" refId="B67" refString="Hannerz, L, 1956. Larval development of the polychaete families Spionidae Sars, Disomidae Mensil, and Poecilochaetidae n. fam. in the Gullmar Fjord (Sweden). Zoologiska bidrag fran Uppsala 31: 1 - 204" title="Larval development of the polychaete families Spionidae Sars, Disomidae Mensil, and Poecilochaetidae n. fam. in the Gullmar Fjord (Sweden)." volume="31" year="1956">Hannerz 1956</bibRefCitation>
|
|
),
|
|
<taxonomicName lsidName="S. bombyx" pageId="0" pageNumber="1" rank="species" species="bombyx">S. cf. bombyx</taxonomicName>
|
|
(
|
|
<bibRefCitation DOI="https://doi.org/10.1201/9781482280159" author="Blake, JA" editor="Rouse, G" journalOrPublisher="Science Publishers, Enfield" pageId="0" pageNumber="1" pagination="565 - 638" refId="B22" refString="Blake, JA, 2006. Chapter 13. Spionida. In: Rouse, G, Pleijel, F, Eds., Reproductive Biology and Phylogeny of Annelida. Science Publishers, Enfield: 565 - 638, DOI: https://doi.org/10.1201/9781482280159" title="Chapter 13. Spionida." url="https://doi.org/10.1201/9781482280159" volumeTitle="Reproductive Biology and Phylogeny of Annelida." year="2006">Blake 2006</bibRefCitation>
|
|
), and
|
|
<taxonomicName lsidName="S. duplex" pageId="0" pageNumber="1" rank="species" species="duplex">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">S. duplex</emphasis>
|
|
</taxonomicName>
|
|
(Chamberlin, 1919) (
|
|
<bibRefCitation DOI="https://doi.org/10.1201/9781482280159" author="Blake, JA" editor="Rouse, G" journalOrPublisher="Science Publishers, Enfield" pageId="0" pageNumber="1" pagination="565 - 638" refId="B22" refString="Blake, JA, 2006. Chapter 13. Spionida. In: Rouse, G, Pleijel, F, Eds., Reproductive Biology and Phylogeny of Annelida. Science Publishers, Enfield: 565 - 638, DOI: https://doi.org/10.1201/9781482280159" title="Chapter 13. Spionida." url="https://doi.org/10.1201/9781482280159" volumeTitle="Reproductive Biology and Phylogeny of Annelida." year="2006">Blake 2006</bibRefCitation>
|
|
). However, the illustrations of
|
|
<taxonomicName lsidName="S. bombyx" pageId="0" pageNumber="1" rank="species" species="bombyx">S. cf. bombyx</taxonomicName>
|
|
provided by
|
|
<bibRefCitation DOI="https://doi.org/10.1201/9781482280159" author="Blake, JA" editor="Rouse, G" journalOrPublisher="Science Publishers, Enfield" pageId="0" pageNumber="1" pagination="565 - 638" refId="B22" refString="Blake, JA, 2006. Chapter 13. Spionida. In: Rouse, G, Pleijel, F, Eds., Reproductive Biology and Phylogeny of Annelida. Science Publishers, Enfield: 565 - 638, DOI: https://doi.org/10.1201/9781482280159" title="Chapter 13. Spionida." url="https://doi.org/10.1201/9781482280159" volumeTitle="Reproductive Biology and Phylogeny of Annelida." year="2006">Blake (2006</bibRefCitation>
|
|
: fig. 13.10C, D) are seemingly more similar to larvae of
|
|
<taxonomicName authorityName="Hartman" authorityYear="1936" class="Polychaeta" family="Spionidae" genus="Rhynchospio" kingdom="Animalia" lsidName="Rhynchospio" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Rhynchospio</emphasis>
|
|
</taxonomicName>
|
|
than to those of
|
|
<taxonomicName authorityName="Grube" authorityYear="1860" class="Polychaeta" family="Spionidae" genus="Spiophanes" kingdom="Animalia" lsidName="Spiophanes" order="Spionida" pageId="0" pageNumber="1" phylum="Annelida" rank="genus">
|
|
<emphasis italics="true" pageId="0" pageNumber="1">Spiophanes</emphasis>
|
|
</taxonomicName>
|
|
.
|
|
</paragraph>
|
|
</subSubSection>
|
|
</treatment>
|
|
</document> |