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<document id="B571E876F6D7BA3B67BD6728770DDF59" ID-CLB-Dataset="55976" ID-DOI="10.1093/zoolinnean/zlab074" ID-GBIF-Dataset="1accd17e-31dd-4bff-ad55-6dfb8872ca43" ID-ISSN="0024-4082" ID-Zenodo-Dep="6461266" ID-ZooBank="53637D76-285D-4AB8-9E52-6CDB6F6738D3" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1649833648776" checkinUser="plazi" docAuthor="González-Miguéns, Rubén, Soler-Zamora, Carmen, Villar-Depablo, Mar, Todorov, Milcho &amp; Lara, Enrique" docDate="2022" docId="1C22923F294E0972FC6D4E0AFD48FC49" docLanguage="en" docName="zlab074.pdf" docOrigin="Zoological Journal of the Linnean Society 194 (4)" docStyle="DocumentStyle:36B3BD6A90C22AB4F7F465C853188CC8.7:ZoolJLinnSoc.2017-2023.journal_article" docStyleId="36B3BD6A90C22AB4F7F465C853188CC8" docStyleName="ZoolJLinnSoc.2017-2023.journal_article" docStyleVersion="7" docTitle="Arcella guadarramensis González-Miguéns, Soler-Zamora, Villar-Depablo, Todorov &amp; Lara, 2022, SP. NOV." docType="treatment" docVersion="6" lastPageNumber="1066" masterDocId="E01BEA47295B0964FF434D5AFFE6FFC5" masterDocTitle="Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology" masterLastPageNumber="1071" masterPageNumber="1044" pageNumber="1065" updateTime="1701998958500" updateUser="plazi">
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<mods:title id="68C71FC49F259A1D832FE21032557410">Multiple convergences in the evolutionary history of the testate amoeba family Arcellidae (Amoebozoa: Arcellinida: Sphaerothecina): when the ecology rules the morphology</mods:title>
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<mods:namePart id="DCD64FC54567F5307217644FFBED071D">González-Miguéns, Rubén</mods:namePart>
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<mods:namePart id="51DB5FC93601786B81E963D2061925E6">Soler-Zamora, Carmen</mods:namePart>
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<mods:namePart id="D63F3EE2A2B877A5CAD23801AAEA0675">Villar-Depablo, Mar</mods:namePart>
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<mods:namePart id="595F3DFCFD3284B5E1CF2521ECEA226A">Todorov, Milcho</mods:namePart>
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<mods:namePart id="618527545E808132B44138E48D4E62E3">Lara, Enrique</mods:namePart>
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<mods:title id="46E1F6E57BA6B93A3889D7FF7F18E55D">Zoological Journal of the Linnean Society</mods:title>
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<mods:date id="E90BA6622A6E93E83DA40DE40EF5E8EA">2022</mods:date>
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<mods:number id="7383B3DDA72A872E73A27CB83744D994">2021-10-26</mods:number>
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<mods:number id="4BC7D4B33249208BEA68B20F4B7DC74D">194</mods:number>
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<mods:identifier id="17AC77021B379CC47D8D5934126CCA17" type="DOI">10.1093/zoolinnean/zlab074</mods:identifier>
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<treatment id="1C22923F294E0972FC6D4E0AFD48FC49" ID-DOI="http://doi.org/10.5281/zenodo.6461317" ID-GBIF-Taxon="194598678" ID-Zenodo-Dep="6461317" LSID="urn:lsid:plazi:treatment:1C22923F294E0972FC6D4E0AFD48FC49" httpUri="http://treatment.plazi.org/id/1C22923F294E0972FC6D4E0AFD48FC49" lastPageId="22" lastPageNumber="1066" pageId="21" pageNumber="1065">
<subSubSection id="DC9170A2294E0971FC6D4E0AFB55FC42" pageId="21" pageNumber="1065" type="nomenclature">
<paragraph id="94342329294E0971FC6D4E0AFB55FC42" blockId="21.[814,1420,848,903]" pageId="21" pageNumber="1065">
<heading id="CF7C9445294E0971FC6D4E0AFB55FC42" centered="true" fontSize="9" level="2" pageId="21" pageNumber="1065" reason="3">
<taxonomicName id="538B58AA294E0971FC6D4E0AFBA2FC43" authorityName="González-Miguéns &amp; Soler-Zamora &amp; Villar-Depablo &amp; Todorov &amp; Lara" authorityYear="2022" class="Lobosa" family="Arcellidae" genus="Arcella" kingdom="Protozoa" order="Arcellinida" pageId="21" pageNumber="1071" phylum="Amoebozoa" rank="species" species="guadarramensis" status="sp. nov.">
<emphasis id="A6FFFF3B294E0971FC6D4E0AFB9AFCA3" bold="true" box="[814,1148,848,872]" italics="true" pageId="21" pageNumber="1065">ARCELLA GUADARRAMENSIS</emphasis>
GONZÁLEZ- MIGUÉNS &amp; LARA
</taxonomicName>
,
<taxonomicNameLabel id="BDCC4240294E0971FB124E28FB55FC42" box="[1105,1203,878,903]" pageId="21" pageNumber="1065" rank="species">
<emphasis id="A6FFFF3B294E0971FB124E28FB4AFC40" bold="true" box="[1105,1196,879,903]" pageId="21" pageNumber="1065">SP. NOV</emphasis>
.
</taxonomicNameLabel>
</heading>
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294E0971FB714ECFFA66FC37" pageId="21" pageNumber="1065" type="description">
<paragraph id="94342329294E0971FB714ECFFB61FC6B" blockId="21.[1074,1159,917,942]" box="[1074,1159,917,942]" pageId="21" pageNumber="1065">
(
<figureCitation id="0CB03FAC294E0971FB784ECFFB99FC6B" box="[1083,1151,917,942]" captionStart="Figure 8" captionStartId="16.[163,242,882,904]" captionTargetBox="[163,1442,186,842]" captionTargetId="figure-418@16.[162,1442,185,843]" captionTargetPageId="16" captionText="Figure 8. Arcella guadarramensis: scanning electron micrographs of the aboral and oral sides of the test. The images on the right represent a detail of the test and the structure of the aperture. On the left, a photograph of a typical habitat for this species, and original drawing of the closest resembling species, Galeripora artocrea (Leidy, 1879)." figureDoi="http://doi.org/10.5281/zenodo.6461282" httpUri="https://zenodo.org/record/6461282/files/figure.png" pageId="21" pageNumber="1065">FIG. 8</figureCitation>
)
</paragraph>
<paragraph id="94342329294E0971FC6A4EE4FA66FC37" blockId="21.[809,1426,958,1010]" pageId="21" pageNumber="1065">
<emphasis id="A6FFFF3B294E0971FC6A4EE4FB91FC16" box="[809,1143,958,979]" italics="true" pageId="21" pageNumber="1065">Z o o b a n k r e g i s t r a t i o n:</emphasis>
u r n: l s i d: z o o b a n k. org:act:
<uuid id="E02D19FC294E0971FC344E86FA9AFC37" box="[887,1404,988,1010]" pageId="21" pageNumber="1065">57A3452C-3C46-44F2-A5C9-48496E34BB57</uuid>
.
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294E0971FC6A4941FBB8FBF4" box="[809,1118,1051,1073]" pageId="21" pageNumber="1065" type="materials_examined">
<paragraph id="94342329294E0971FC6A4941FBB8FBF4" blockId="21.[809,1118,1051,1073]" box="[809,1118,1051,1073]" pageId="21" pageNumber="1065">
<materialsCitation id="24E32974294E0971FC6A4941FBB8FBF4" ID-GBIF-Occurrence="3751567304" box="[809,1118,1051,1073]" collectionCode="MA-Algae" pageId="21" pageNumber="1065" specimenCode="MA-Algae11251" specimenCount="1" typeStatus="holotype">
<emphasis id="A6FFFF3B294E0971FC6A4941FC75FBF5" box="[809,915,1051,1072]" italics="true" pageId="21" pageNumber="1065">
<typeStatus id="4B309D8B294E0971FC6A4941FC6BFBF5" box="[809,909,1051,1072]" pageId="21" pageNumber="1065" type="holotype">Holotype</typeStatus>
:
</emphasis>
<specimenCode id="C42D8B52294E0971FCDC4941FBBFFBF4" box="[927,1113,1051,1073]" collectionCode="MA-Algae" pageId="21" pageNumber="1065">MA-Algae11251</specimenCode>
.
</materialsCitation>
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294E0971FC6A4900FC8DF9F9" pageId="21" pageNumber="1065" type="diagnosis">
<paragraph id="94342329294E0971FC6A4900FC8DF9F9" blockId="21.[809,1425,1114,1596]" pageId="21" pageNumber="1065">
<emphasis id="A6FFFF3B294E0971FC6A4900FBEFFBAA" box="[809,1033,1114,1135]" italics="true" pageId="21" pageNumber="1065">Specific diagnosis:</emphasis>
Test diameter: clade L: 114.60 125.90 µm, average 120.30 µm (
<emphasis id="A6FFFF3B294E0971FBC84923FB79FB4B" box="[1163,1183,1145,1166]" italics="true" pageId="21" pageNumber="1065">N</emphasis>
= 6); aperture 19.60 30.00 µm, average 23.63 µm. clade M 141.50149.95 µm, average 146.06 µm (
<emphasis id="A6FFFF3B294E0971FB5049ECFBC1FB0E" box="[1043,1063,1206,1227]" italics="true" pageId="21" pageNumber="1065">N</emphasis>
= 4); aperture 31.2534.50 µm, average 33.08 µm. Besides a difference in size, both clades have an identical morphology. Colour ranges from transparent to yellow-orange. Subhemispherical test shape, with flattened edges and dimples in the surface that gives the test a golf ball shape. No ribs or keels on the aboral side. Hexagonal building units are visible, which gives the test a rough appearance; little pores can be seen at the vertices. Building units can also be appreciated at the oral side of the test, with pores at the vertices and a central aperture. The aperture is invaginated outwards forming a short ring or lip.
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294E0971FC6A4B28FB9AF8C7" pageId="21" pageNumber="1065" type="discussion">
<paragraph id="94342329294E0971FC6A4B28FB9AF8C7" blockId="21.[809,1425,1650,1794]" pageId="21" pageNumber="1065">
<emphasis id="A6FFFF3B294E0971FC6A4B28FBBDF942" box="[809,1115,1650,1671]" italics="true" pageId="21" pageNumber="1065">Intraspecific variability:</emphasis>
The building units may vary slightly in shape. Some building units may be collapsed, giving a rough surface. There may be certain deformations in the test that prevent it from having a perfectly circular morphology.
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294E0972FC6A4A76FF34FE56" lastPageId="22" lastPageNumber="1066" pageId="21" pageNumber="1065" type="diagnosis">
<paragraph id="94342329294E0972FC6A4A76FF34FE56" blockId="21.[809,1425,1835,1888]" lastBlockId="22.[163,780,197,403]" lastPageId="22" lastPageNumber="1066" pageId="21" pageNumber="1065">
<emphasis id="A6FFFF3B294E0971FC6A4A76FC3DF89A" italics="true" pageId="21" pageNumber="1065">
Diagnosis with closely related species:
<taxonomicName id="538B58AA294E0971FA754A71FC3DF89A" authorityName="González-Miguéns &amp; Soler-Zamora &amp; Villar-Depablo &amp; Todorov &amp; Lara" authorityYear="2022" class="Lobosa" family="Arcellidae" genus="Arcella" kingdom="Protozoa" order="Arcellinida" pageId="21" pageNumber="1065" phylum="Amoebozoa" rank="species" species="guadarramensis">Arcella guadarramensis</taxonomicName>
</emphasis>
can be diagnosed by its specific sequences of the mtDNA markers and by its phylogenetic placement.
<taxonomicName id="538B58AA294D0972FFE04DBEFE5FFF3C" authorityName="González-Miguéns &amp; Soler-Zamora &amp; Villar-Depablo &amp; Todorov &amp; Lara" authorityYear="2022" box="[163,441,228,249]" class="Lobosa" family="Arcellidae" genus="Arcella" kingdom="Protozoa" order="Arcellinida" pageId="22" pageNumber="1066" phylum="Amoebozoa" rank="species" species="guadarramensis">
<emphasis id="A6FFFF3B294D0972FFE04DBEFE5FFF3C" box="[163,441,228,249]" italics="true" pageId="22" pageNumber="1066">Arcella guadarramensis</emphasis>
</taxonomicName>
differs morphologically from similar-looking
<taxonomicName id="538B58AA294D0972FE204C59FE39FED2" authorityName="González-Miguéns &amp; Soler-Zamora &amp; Villar-Depablo &amp; Todorov &amp; Lara" authorityYear="2022" box="[355,479,258,280]" class="Lobosa" family="Arcellidae" genus="Galeripora" kingdom="Protozoa" order="Arcellinida" pageId="22" pageNumber="1066" phylum="Amoebozoa" rank="species" species="succelli">
<emphasis id="A6FFFF3B294D0972FE204C59FE90FEDD" box="[355,374,259,280]" italics="true" pageId="22" pageNumber="1066">G</emphasis>
.
<emphasis id="A6FFFF3B294D0972FEC44C58FE39FED2" box="[391,479,258,279]" italics="true" pageId="22" pageNumber="1066">succelli</emphasis>
</taxonomicName>
by (1) its morphometric differences with
<taxonomicName id="538B58AA294D0972FE294C7BFE05FEF3" authorityName="González-Miguéns &amp; Soler-Zamora &amp; Villar-Depablo &amp; Todorov &amp; Lara" authorityYear="2022" box="[362,483,289,311]" class="Lobosa" family="Arcellidae" genus="Galeripora" kingdom="Protozoa" order="Arcellinida" pageId="22" pageNumber="1066" phylum="Amoebozoa" rank="species" species="succelli">
<emphasis id="A6FFFF3B294D0972FE294C7BFE9BFEF3" box="[362,381,289,310]" italics="true" pageId="22" pageNumber="1066">G</emphasis>
.
<emphasis id="A6FFFF3B294D0972FECE4C7BFE05FEF3" box="[397,483,289,310]" italics="true" pageId="22" pageNumber="1066">succelli</emphasis>
</taxonomicName>
(see Morphometrics and morphology;
<figureCitation id="0CB03FAC294D0972FE7B4C1AFE9FFE90" box="[312,377,320,342]" captionStart="Figure 2" captionStartId="9.[145,224,1219,1241]" captionTargetBox="[228,1342,193,1173]" captionTargetId="figure-249@9.[225,1345,190,1180]" captionTargetPageId="9" captionText="Figure 2. A, scatterplot of the scores of linear discriminants with x-axis representing discriminant function 1 (LD1) and y-axis representing discriminant function 2 (LD2). Colours represent the different mitochondrial clades and symbols refer to the different sections after Deflandre (1928): squares are for Section 1 Vulgares, circles for Section 2 Carinatae and triangles for Section 3 Aplanatae. The drawings represent the different morphotypes. B, the table represents the results of a linear discriminant analysis which determines the relationship between predicted and observed specimens cells for each mitochondrial clade." figureDoi="http://doi.org/10.5281/zenodo.6461270" httpUri="https://zenodo.org/record/6461270/files/figure.png" pageId="22" pageNumber="1066">Fig. 2</figureCitation>
), both, clade N and O, are notably smaller than
<taxonomicName id="538B58AA294D0972FE744C05FE40FEB6" authorityName="González-Miguéns &amp; Soler-Zamora &amp; Villar-Depablo &amp; Todorov &amp; Lara" authorityYear="2022" box="[311,422,350,372]" class="Lobosa" family="Arcellidae" genus="Galeripora" kingdom="Protozoa" order="Arcellinida" pageId="22" pageNumber="1066" phylum="Amoebozoa" rank="species" species="succelli">
<emphasis id="A6FFFF3B294D0972FE744C05FE40FEB6" box="[311,422,350,372]" italics="true" pageId="22" pageNumber="1066">G. succelli</emphasis>
</taxonomicName>
and (2) the rough outlook of the test.
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294D0972FFE04C94FD81FE21" box="[163,615,462,484]" pageId="22" pageNumber="1066" type="biology_ecology">
<paragraph id="94342329294D0972FFE04C94FD81FE21" blockId="22.[163,615,462,484]" box="[163,615,462,484]" pageId="22" pageNumber="1066">
<emphasis id="A6FFFF3B294D0972FFE04C94FEE7FE26" box="[163,257,462,483]" italics="true" pageId="22" pageNumber="1066">Habitat:</emphasis>
Wet
<taxonomicName id="538B58AA294D0972FE024C94FE58FE26" box="[321,446,462,483]" class="Sphagnopsida" family="Sphagnaceae" genus="Sphagnum" kingdom="Plantae" order="Sphagnales" pageId="22" pageNumber="1066" phylum="Bryophyta" rank="genus">
<emphasis id="A6FFFF3B294D0972FE024C94FE58FE26" box="[321,446,462,483]" italics="true" pageId="22" pageNumber="1066">Sphagnum</emphasis>
</taxonomicName>
moss, in a fen.
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294D0972FFE04F4CFE8DFD8F" pageId="22" pageNumber="1066" type="materials_examined">
<paragraph id="94342329294D0972FFE04F4CFE8DFD8F" blockId="22.[163,777,533,586]" pageId="22" pageNumber="1066">
<emphasis id="A6FFFF3B294D0972FFE04F4CFEA1FDEF" box="[163,327,533,555]" italics="true" pageId="22" pageNumber="1066">
<typeStatus id="4B309D8B294D0972FFE04F4CFF3DFDEE" box="[163,219,534,555]" pageId="22" pageNumber="1066">Type</typeStatus>
locality:
</emphasis>
<collectingCountry id="EC9C63B9294D0972FE164F4FFE78FDEE" box="[341,414,533,555]" name="Spain" pageId="22" pageNumber="1066">Spain</collectingCountry>
,
<collectingRegion id="564FEDCB294D0972FEEE4F4FFDEFFDEE" box="[429,521,533,555]" country="Spain" name="Madrid" pageId="22" pageNumber="1066">Madrid</collectingRegion>
, Puerto de Canencia (
<geoCoordinate id="F1BF45EE294D0972FFE94F6EFEE3FD8F" box="[170,261,564,586]" degrees="40" direction="north" minutes="52" orientation="latitude" pageId="22" pageNumber="1066" precision="925" value="40.866665">40°52N</geoCoordinate>
<geoCoordinate id="F1BF45EE294D0972FE4F4F6EFEB9FD8F" box="[268,351,564,586]" degrees="3" direction="west" minutes="45" orientation="longitude" pageId="22" pageNumber="1066" precision="925" value="-3.75">3°45W</geoCoordinate>
).
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294D0972FFE04F21FD90FCCE" pageId="22" pageNumber="1066" type="etymology">
<paragraph id="94342329294D0972FFE04F21FD90FCCE" blockId="22.[163,780,634,779]" pageId="22" pageNumber="1066">
<emphasis id="A6FFFF3B294D0972FFE04F21FED1FD55" box="[163,311,634,656]" italics="true" pageId="22" pageNumber="1066">Etymology:</emphasis>
The name is derived from River Guadarrama, a river with a name of Arabic roots:
<emphasis id="A6FFFF3B294D0972FD8F4FC3FCE3FD6B" box="[716,773,665,686]" italics="true" pageId="22" pageNumber="1066">wadi</emphasis>
, river, and
<emphasis id="A6FFFF3B294D0972FE594FE2FE9EFD08" box="[282,376,696,717]" italics="true" pageId="22" pageNumber="1066">ar-rama</emphasis>
, sandy. We propose this name as a reference to the
<typeStatus id="4B309D8B294D0972FE164F8DFE60FD29" box="[341,390,727,748]" pageId="22" pageNumber="1066">type</typeStatus>
locality in Sierra de Guadarrama, a mountain range named after this river.
</paragraph>
</subSubSection>
<subSubSection id="DC9170A2294D0972FFF84E60FD48FC49" pageId="22" pageNumber="1066" type="discussion">
<paragraph id="94342329294D0972FFF84E60FD48FC49" blockId="22.[163,779,825,908]" pageId="22" pageNumber="1066">
We provide a key (Supporting Information, Table S3) and a new figure (Supporting Information,
<figureCitation id="0CB03FAC294D0972FDD64E02FD03FCA8" box="[661,741,856,878]" captionStart="Figure 3" captionStartId="11.[145,225,892,914]" captionTargetBox="[145,1425,195,852]" captionTargetId="figure-509@11.[145,1425,195,852]" captionTargetPageId="11" captionText="Figure 3. Galeripora succelli: scanning electron micrographs of the aboral and oral sides of the test. The images on the right represent a detail of the test and the structure of the aperture. Below left, a photograph of a typical habitat for the species, a peat bog. Above left, original drawing of the closest known resembling species, Galeripora artocrea (Leidy, 1879)." figureDoi="http://doi.org/10.5281/zenodo.6461272" httpUri="https://zenodo.org/record/6461272/files/figure.png" pageId="22" pageNumber="1066">Fig. S3</figureCitation>
) to facilitate the identification of the new species.
</paragraph>
</subSubSection>
</treatment>
</document>