282 lines
55 KiB
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282 lines
55 KiB
XML
<document id="C6FC446805FA43E8C6AAC0E53D9C53E2" ID-DOI="10.1093/zoolinnean/zlad044" ID-ISSN="0024-4082" ID-Zenodo-Dep="8326400" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_approvedBy="valdenar" IM.taxonomicNames_approvedBy="valdenar" IM.treatments_approvedBy="felipe" checkinTime="1693804868235" checkinUser="plazi" docAuthor="Song, Wenya, Dong, Jingyi, Lu, Xiaoteng, Al-Farraj, Saleh A., Song, Weibo, Hines, Hunter N. & Luo, Xiaotian" docDate="2023" docId="03D68792FFF0FF81037A3271FC1D8CF8" docLanguage="en" docName="zlad044.pdf" docOrigin="Zoological Journal of the Linnean Society 199 (1)" docSource="http://dx.doi.org/10.1093/zoolinnean/zlad044" docStyle="DocumentStyle:4F230B9370E98E256D973D6DFB57F36C.1:ZoolJLinnSoc.2023-.journal_article" docStyleId="4F230B9370E98E256D973D6DFB57F36C" docStyleIdOld="36B3BD6A90C22AB4F7F465C853188CC8" docStyleName="ZoolJLinnSoc.2023-.journal_article" docStyleNameOld="ZoolJLinnSoc.2017-.journal_article" docStyleOld="DocumentStyle:36B3BD6A90C22AB4F7F465C853188CC8.7:ZoolJLinnSoc.2017-.journal_article" docStyleVersion="1" docStyleVersionOld="7" docTitle="Deviata Eigner 1995" docType="treatment" docVersion="3" lastPageNumber="271" masterDocId="FFEFFFEAFFF6FF89076A3676FF8A8B56" masterDocTitle="Morphological, ontogenetic, and molecular investigations of freshwater hypotrich ciliates from China revealed a new genus Heterodeviata gen. nov. (Protista: Ciliophora), and a novel limnetic population of Deviata multilineae" masterLastPageNumber="279" masterPageNumber="263" pageNumber="269" updateTime="1694436162373" updateUser="valdenar">
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<mods:title id="93CE9027CC555677ABA638B08BB247F5">Morphological, ontogenetic, and molecular investigations of freshwater hypotrich ciliates from China revealed a new genus Heterodeviata gen. nov. (Protista: Ciliophora), and a novel limnetic population of Deviata multilineae</mods:title>
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<mods:namePart id="FF8367C4A2240AA4087BB362D6FDD9D7">Song, Wenya</mods:namePart>
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<mods:affiliation id="642DA3BB862CEC93ED58F8AC664CDFF1">Institute of Evolution & Marine Biodiversity, Ocean University of China, ºingdao 266003, China & Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</mods:affiliation>
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<mods:namePart id="7EECD17B45F71A7CD5D577C4C167FDFD">Dong, Jingyi</mods:namePart>
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<mods:affiliation id="57824BB34E7D2C2D9C7EBB9B436043D9">Institute of Evolution & Marine Biodiversity, Ocean University of China, ºingdao 266003, China</mods:affiliation>
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<mods:namePart id="FD540BECFC1771C69FD7B7C2293C0618">Lu, Xiaoteng</mods:namePart>
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<mods:affiliation id="B2300E2712CF19F5EDD6956E242FC591">Department of Biology, Shenzhen MSU-BIT University, Shenzhen 518172, China</mods:affiliation>
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<mods:namePart id="E12DFA340F0E874442B59832B7960B50">Al-Farraj, Saleh A.</mods:namePart>
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<mods:affiliation id="BCB16E9C538718D298440A8202ADD863">Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia</mods:affiliation>
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<mods:namePart id="0798DA5B46EEE745AD6AFB6743042BBF">Song, Weibo</mods:namePart>
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<mods:affiliation id="E9604B96D9ED248B7B5D8C72ACF496B6">Institute of Evolution & Marine Biodiversity, Ocean University of China, ºingdao 266003, China & Laoshan Laboratory, ºingdao 266237, China</mods:affiliation>
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<mods:namePart id="F9093E2BC06C4F1E0DAF7525DD5562F9">Hines, Hunter N.</mods:namePart>
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<mods:affiliation id="70CD92644FA330FCEC14A0FD2B8E4A54">Harbor Branch Oceanographic Institute, Florida Atlantic University, Fort Pierce, Florida, 34946, USA</mods:affiliation>
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<mods:namePart id="F00750DCCD575B001045BFB42AACE75C">Luo, Xiaotian</mods:namePart>
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<mods:affiliation id="294DF506588207B7C55B117C21ABD4C6">Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</mods:affiliation>
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<mods:nameIdentifier id="F7708CE5FDF2181AC8C355630D94713D" type="email">luoxiaotian@ihb.ac.cn</mods:nameIdentifier>
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<mods:date id="CAEBCA71CA83D89EC65076A8ED5E1CF4">2023</mods:date>
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<mods:identifier id="A9608C86F0556549962A95087A73AB4F" type="DOI">10.1093/zoolinnean/zlad044</mods:identifier>
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<subSubSection id="C365650FFFF0FF8F037A3271FB618F77" box="[1040,1259,1031,1057]" pageId="6" pageNumber="269" type="nomenclature">
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<paragraph id="8BC03684FFF0FF8F037A3271FB618F77" blockId="6.[1040,1259,1031,1057]" box="[1040,1259,1031,1057]" pageId="6" pageNumber="269">
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<heading id="D08881E8FFF0FF8F037A3271FB618F77" bold="true" box="[1040,1259,1031,1057]" centered="true" fontSize="11" level="2" pageId="6" pageNumber="269" reason="2">
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<taxonomicName id="4C7F4D07FFF0FF8F037A3271FB618F77" ID-CoL="43W8" ID-ENA="1513334" authority="Eigner, 1995" authorityName="Eigner" authorityYear="1995" box="[1040,1259,1031,1057]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="6" pageNumber="269" phylum="Ciliophora" rank="genus">
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<emphasis id="B90BEA96FFF0FF8F037A3271FB618F77" bold="true" box="[1040,1259,1031,1057]" pageId="6" pageNumber="269">
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<emphasis id="B90BEA96FFF0FF8F037A3271FBEA8F77" bold="true" box="[1040,1120,1031,1057]" italics="true" pageId="6" pageNumber="269">Deviata</emphasis>
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<bibRefCitation id="EFEE4B75FFF0FF8F030F3271FB618F77" author="Eigner P" box="[1125,1259,1031,1057]" pageId="6" pageNumber="269" pagination="341 - 366" refId="ref11239" refString="Eigner P. Divisional morphogenesis in Deviata abbrevescens nov. gen., nov. spec, Neogeneia hortualis nov. gen., nov. spec, and Kahliella simplex (Horvath) corliss and redefinition of the Kahliellidae (ciliophora, hypotrichida). European Journal of Protistology 1995; 31: 341 - 366." type="journal article" year="1995">Eigner, 1995</bibRefCitation>
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</emphasis>
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</taxonomicName>
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</heading>
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</paragraph>
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<paragraph id="8BC03684FFF0FF8F04EA3244FBCC8F98" blockId="6.[825,1475,1073,1982]" pageId="6" pageNumber="269">
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<taxonomicName id="4C7F4D07FFF0FF8F04EA3244FB758F1A" authority="Zhang et al., 2022" authorityName="Zhang" authorityYear="2022" box="[896,1279,1073,1100]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="6" pageNumber="269" phylum="Ciliophora" rank="species" species="multilineae">
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<emphasis id="B90BEA96FFF0FF8F04EA3244FBBD8F1D" box="[896,1079,1073,1099]" italics="true" pageId="6" pageNumber="269">Deviata multilineae</emphasis>
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<bibRefCitation id="EFEE4B75FFF0FF8F03513247FB758F1A" author="Zhang T & Chen Z" box="[1083,1279,1073,1100]" pageId="6" pageNumber="269" pagination="107623" refId="ref13218" refString="Zhang T, Tang º, Chen Z et al. Insights into the phylogeny of the family Deviatidae (Protozoa, Ciliophora, Hypotrichia) based on multi-gene, morphological and ontogenetic information, with the establishment of a new species Deviata multilineae n. sp. Molecular Phylogenetics and Evolution 2022; 177: 107623." type="journal article" year="2022">Zhang et al., 2022</bibRefCitation>
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</taxonomicName>
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<emphasis id="B90BEA96FFF0FF8F026E3244FB008F26" italics="true" pageId="6" pageNumber="269">
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(
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<figureCitation id="13442A01FFF0FF8F02643244FAF88F1A" box="[1294,1394,1074,1100]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="6" pageNumber="269">Fig.7A–N</figureCitation>
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) Deposition of voucher specimens
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</emphasis>
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: Three voucher slides (No. SWY2020051501/1–3) with protargol-stained specimens were deposited in the Laboratory of Protozoology, Ocean University of
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<collectingCountry id="F3687614FFF0FF8F043932C0FC1E8F98" box="[851,916,1206,1230]" name="China" pageId="6" pageNumber="269">China</collectingCountry>
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, ºingdao,
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<collectingCountry id="F3687614FFF0FF8F036A32C0FBCB8F98" box="[1024,1089,1206,1230]" name="China" pageId="6" pageNumber="269">China</collectingCountry>
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.
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</paragraph>
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<paragraph id="8BC03684FFF0FF8F043F32A3FB6D8C77" blockId="6.[825,1475,1073,1982]" pageId="6" pageNumber="269">
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Cell size 150–175 × 40–60 μm (160 × 50 μm on average)
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<emphasis id="B90BEA96FFF0FF8F04533283FCF78E5A" box="[825,893,1269,1292]" italics="true" pageId="6" pageNumber="269">in vivo</emphasis>
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(
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<emphasis id="B90BEA96FFF0FF8F04FB3283FC2E8E5A" box="[913,932,1269,1292]" italics="true" pageId="6" pageNumber="269">N</emphasis>
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= 6), with ratio of length to width
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<emphasis id="B90BEA96FFF0FF8F02423283FAE18E5A" box="[1320,1387,1269,1292]" italics="true" pageId="6" pageNumber="269">in vivo</emphasis>
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ranging from 2.8 to 3.9; about 165 × 83 μm (140–185 × 55–116 μm), length:width ratio about 2.0:1 (1.6–2.9:1) asser protargol impregnation. Cell flexible but not contractile, outline elongate elliptical, usually with anterior part wider than the posterior one (
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<figureCitation id="13442A01FFF0FF8F042E33E7FC638EFF" box="[836,1001,1425,1449]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="6" pageNumber="269">Fig. 7A–D, F–H</figureCitation>
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). Algae in food vacuole rendered cell yellow/ greenish at low magnification (
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<figureCitation id="13442A01FFF0FF8F03EA33C6FA808E9E" box="[1152,1290,1456,1481]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="6" pageNumber="269">Fig. 7A, F–H</figureCitation>
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). Cytoplasm colourless, with numerous colourless globules (0.75–1.5 μm) and many transparent colourless inclusions (2–11 μm), most being spherical and located in the middle of the cell (
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<figureCitation id="13442A01FFF0FF8F026E3078FAD18D70" box="[1284,1371,1550,1574]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="6" pageNumber="269">Fig. 7E, I</figureCitation>
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). Cortical granules absent. Four macronuclear nodules, ellipsoidal-shaped, 15–18 × 13–16 μm
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<emphasis id="B90BEA96FFF0FF8F037A3038FBDB8D33" box="[1040,1105,1614,1637]" italics="true" pageId="6" pageNumber="269">in vivo</emphasis>
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, usually two micronuclei, each one usually located between a pair of macronuclear nodules (
|
||
<figureCitation id="13442A01FFF0FF8F02F6301AFC1A8DF5" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="6" pageNumber="269">Fig. 7I, K, N</figureCitation>
|
||
). Contractile vacuole about 15 μm when full, located at the equatorial level, near less cell margin (
|
||
<figureCitation id="13442A01FFF0FF8F026D30DDFA3B8D95" box="[1287,1457,1707,1731]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="6" pageNumber="269">Fig. 7A, B–D, H</figureCitation>
|
||
). Collecting canals absent. Locomotion by slowly floating in water, sometimes crawling around the substratum. No cell division, cysts, or conjugation were observed.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C365650FFFF0FF81043F315EFC1D8CF8" lastPageId="8" lastPageNumber="271" pageId="6" pageNumber="269" type="description">
|
||
<paragraph id="8BC03684FFF0FF8E043F315EFC2D8D39" blockId="6.[825,1475,1073,1982]" lastBlockId="7.[810,1460,1561,1741]" lastPageId="7" lastPageNumber="270" pageId="6" pageNumber="269">
|
||
The adoral zone of membranelles occupies 28% of cell length in protargol preparations, composed of 23–35 membranelles (
|
||
<tableCitation id="C6FD033FFFF0FF8F042E3111FC108C29" box="[836,922,1895,1919]" captionStart="Table 1" captionStartId="5.[113,168,143,167]" captionText="Table 1. Morphometric data of Heterodeviata sinica (upper line) and Deviata multilineae (lower line)" pageId="6" pageNumber="269">Table 1</tableCitation>
|
||
). Paroral and endoral consisted of single-rowed dikinetids, nearly parallel to each other in most cells (intersected in two out of 15 cells examined), with the former shorter than the laưer (
|
||
<figureCitation id="13442A01FFF1FF8E0673306FFE088D66" box="[281,386,1561,1585]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="7" pageNumber="270">Fig. 7J, M</figureCitation>
|
||
). The cytopharyngeal fibres extend posteriorly from the cytostome (
|
||
<figureCitation id="13442A01FFF1FF8E06AF304EFD8D8D06" box="[453,519,1592,1616]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="7" pageNumber="270">Fig. 7J</figureCitation>
|
||
). Length of cirri
|
||
<emphasis id="B90BEA96FFF1FF8E05D3304FFD708D06" box="[697,762,1593,1616]" italics="true" pageId="7" pageNumber="270">in vivo</emphasis>
|
||
about 11–13 μm. Three frontal cirri, usually one buccal cirrus located right to the anterior end of the paroral and anterior of the endoral (two buccal cirri observed in three out of 20 cells), three to seven parabuccal cirri behind the right frontal cirrus (
|
||
<figureCitation id="13442A01FFF1FF8E071630A3FE828DBA" box="[124,264,1749,1773]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="7" pageNumber="270">Fig. 7J, M, N</figureCitation>
|
||
). Three frontoventral cirral rows, frontoventral cirral row 1 (FVR1) commences at right of right frontal cirrus and terminates at posterior 15% of cell length. In six out of 15 cells, FVR1 consisting of two parts: the anterior part terminates behind the proximal end of AZM, the posterior part commences at the right of several last cirri of anterior part (
|
||
<figureCitation id="13442A01FFF1FF8E05D93107FF0F8CFE" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="7" pageNumber="270">Fig. 7J, N</figureCitation>
|
||
). Another two frontoventral cirral rows terminate at the rear end of the cell (
|
||
<figureCitation id="13442A01FFF1FF8E067031C6FEF78C91" box="[282,381,1968,1992]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="7" pageNumber="270">Fig. 7J, N</figureCitation>
|
||
). Eight to eleven less marginal rows, most of them located on the dorsal side; two or three right marginal rows, most of them distributed on the ventral side (
|
||
<figureCitation id="13442A01FFF1FF8E02E7304EFC1D8D39" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="7" pageNumber="270">Fig. 7J, K, L, N</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<caption id="DF00660CFFF1FF8E071B337BFADC8EBF" ID-DOI="http://doi.org/10.5281/zenodo.8326408" ID-Zenodo-Dep="8326408" httpUri="https://zenodo.org/record/8326408/files/figure.png" pageId="7" pageNumber="270" startId="7.[113,178,1293,1317]" targetBox="[154,1418,144,1264]" targetPageId="7" targetType="figure">
|
||
<paragraph id="8BC03684FFF1FF8E071B337BFA178EC3" blockId="7.[113,1447,1293,1513]" pageId="7" pageNumber="270">
|
||
<emphasis id="B90BEA96FFF1FF8E071B337BFF438E73" bold="true" box="[113,201,1293,1317]" pageId="7" pageNumber="270">Figure 4.</emphasis>
|
||
Early to middle morphogenetic stages of
|
||
<taxonomicName id="4C7F4D07FFF1FF8E0525337BFC8C8E73" authorityName="Song & Dong & Lu & Al-Farraj & Song & Hines & Luo" authorityYear="2023" box="[591,774,1293,1317]" genus="Heterodeviata" pageId="7" pageNumber="270" rank="species" species="sinica">
|
||
<emphasis id="B90BEA96FFF1FF8E0525337BFC8C8E73" box="[591,774,1293,1317]" italics="true" pageId="7" pageNumber="270">Heterodeviata sinica</emphasis>
|
||
</taxonomicName>
|
||
asser protargol impregnation. A, macronuclear nodules of a very early divider (ventral view), showing replication band of macronuclear nodules (arrows). B, oral primordium of the opisthe (arrow). C, developed oral primordia (arrow). D–F, ventrolateral (D, E) and dorsolateral (F) view of the same early divider, showing incomplete adoral zone of membranelles (arrow in D, E), the intrakinetally formed marginal anlagen and dorsal kinety anlage 1. G, H, ventral (G) and dorsal (H) view of the same early-middle divider, showing the dorsal kinety 2 anlage formed to the right of the right marginal anlage 2. I, J, ventral (I) and dorsal
|
||
</paragraph>
|
||
<paragraph id="8BC03684FFF1FF8E071B33EFFADC8EBF" blockId="7.[113,1447,1293,1513]" pageId="7" pageNumber="270">(J) view of the same middle divider, denoting anlagen I–IV differentiating into cirri, arrowhead in (I) shows the parabuccal cirrus. DḎ1, 2, dorsal kineties anlagen 1, 2; FC, frontal cirri; FVR, frontoventral cirral row; LMA1, 2, less marginal anlagen 1, 2; Ma, macronuclear nodules; Mi, micronuclei; RMA1, 2, right marginal anlagen 1, 2; UMA, undulating membrane anlage. Scale bars: 20 μm (A–C), 50 μm (D–J).</paragraph>
|
||
</caption>
|
||
<paragraph id="8BC03684FFF1FF8E042F3001FAFD8D9B" blockId="7.[810,1460,1561,1741]" pageId="7" pageNumber="270">
|
||
Three long dorsal kineties (DK), dorsal bristles inconspicuous
|
||
<emphasis id="B90BEA96FFF1FF8E044030E1FCE18DF8" box="[810,875,1687,1710]" italics="true" pageId="7" pageNumber="270">in vivo</emphasis>
|
||
. DK1 with 9–17 dikinetids, DK2 with 17–27 dikinetids, DK3 with 11–17 dikinetids. Caudal cirri absent (
|
||
<figureCitation id="13442A01FFF1FF8E027730C3FAED8D9B" box="[1309,1383,1717,1741]" captionStart="Figure 7" captionStartId="10.[129,194,1159,1183]" captionTargetBox="[171,1422,147,1129]" captionTargetId="figure-365@10.[171,1432,144,1131]" captionTargetPageId="10" captionText="Figure 7. Deviata multilineae from life (A–I) and asser protargol impregnation (J–N). A, F, ventral view of a representative individual, arrowhead in (F) marks the algae in the cell. B–D, G, H, ventral views of different cells, arrowheads indicate contractile vacuole.E, ventral view, showing the detail of cirri and their surrounded globules.I, denoting the macronuclear nodules and the cytoplasm.J, K, N, ventral (J, N) and dorsal (K) view of the same representative cell, showing infraciliature and nuclear apparatus, arrowhead in (J) marks the buccal cirrus, arrows and arrowheads in (K, N) indicate macronuclear nodules and micronuclei, respectively.L, dorsal view to show the less marginal rows on dorsal side. M, ventral view, arrowhead indicates buccal cirrus. 1–3, dorsal kineties 1–3; E, endoral; FC, frontal cirri; FVR1–3, frontoventral cirral rows 1–3; LMR, less marginal row; Ma, macronuclear nodules; P, paroral; PBC, parabuccal cirri; RMR, right marginal row. Scale bars: 50 μm." figureDoi="http://doi.org/10.5281/zenodo.8326414" httpUri="https://zenodo.org/record/8326414/files/figure.png" pageId="7" pageNumber="270">Fig. 7K</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BC03684FFF1FF8E04E1309BFB588C70" blockId="7.[810,1459,1773,1992]" pageId="7" pageNumber="270">
|
||
<emphasis id="B90BEA96FFF1FF8E04E1309BFB588C70" bold="true" pageId="7" pageNumber="270">
|
||
18S rRNA gene sequence and phylogenetic analyses (
|
||
<figureCitation id="13442A01FFF1FF8E0318317BFB3A8C71" box="[1138,1200,1805,1831]" captionStart="Figure 8" captionStartId="11.[113,178,996,1020]" captionTargetBox="[155,1418,144,969]" captionTargetId="figure-362@11.[154,1418,144,969]" captionTargetPageId="11" captionText="Figure 8. Nucleotide differences between deviatids based on 18S rRNA gene sequences.In (A), the lower less values of the table indicate the sequence similarity, the upper right numbers are numbers of nucleotide differences.The numbers in the header (B) indicate the unmatched site positions. In A, B, the newly obtained sequences are in red.‘**’ indicates the sequence Perisincirra sp. (KY855575) which was likely misidentified and can be considered conspecific with Deviata brasiliensis. ‘H’,Heterodeviata." figureDoi="http://doi.org/10.5281/zenodo.8326416" httpUri="https://zenodo.org/record/8326416/files/figure.png" pageId="7" pageNumber="270">Figs 8</figureCitation>
|
||
,
|
||
<figureCitation id="13442A01FFF1FF8E03D3317BFB4D8C71" box="[1209,1223,1805,1831]" captionStart="Figure 9" captionStartId="12.[129,194,1089,1113]" captionTargetBox="[172,1430,147,1059]" captionTargetId="figure-439@12.[169,1433,144,1062]" captionTargetPageId="12" captionText="Figure 9. Comparison of Heterodeviata with related genera within Deviatidae and Kahllidae that possess three frontal cirri, at least one short or long frontal ventral cirral row right of the cell midline, at least one less marginal row, and one dorsomarginal kinety.Figures in the upper and lower row show the dorsal kinety paưern and cirral paưern of corresponding genera, respectively. Old (parental) structures (marginal cirri or dorsal kineties) are depicted by contour.Abbreviations:BC, buccal cirri; CC, caudal cirri; DK, dorsal kinety; FC, frontal cirri; FVR, frontoventral cirral row; LMR, less marginal row; PBC, parabuccal cirri; RMR, right marginal row; TC, transverse cirri." figureDoi="http://doi.org/10.5281/zenodo.8326418" httpUri="https://zenodo.org/record/8326418/files/figure.png" pageId="7" pageNumber="270">9</figureCitation>
|
||
)
|
||
</emphasis>
|
||
</paragraph>
|
||
<paragraph id="8BC03684FFF1FF8E04403145FC288C9E" blockId="7.[810,1459,1773,1992]" pageId="7" pageNumber="270">
|
||
The two new 18S rRNA gene sequences obtained in this study were deposited in GenBank. Their lengths, GC-content, and accession numbers are as follows:
|
||
<taxonomicName id="4C7F4D07FFF1FF8E03CD3104FAF98CDC" authorityName="Song & Dong & Lu & Al-Farraj & Song & Hines & Luo" authorityYear="2023" box="[1191,1395,1906,1930]" genus="Heterodeviata" pageId="7" pageNumber="270" rank="species" species="sinica">
|
||
<emphasis id="B90BEA96FFF1FF8E03CD3104FAF98CDC" box="[1191,1395,1906,1930]" italics="true" pageId="7" pageNumber="270">Heterodeviata sinica</emphasis>
|
||
</taxonomicName>
|
||
1571 bp, 45.59%, OP537913;
|
||
<taxonomicName id="4C7F4D07FFF1FF8E035A31E4FB7B8CFF" box="[1072,1265,1937,1961]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="7" pageNumber="270" phylum="Ciliophora" rank="species" species="multilineae">
|
||
<emphasis id="B90BEA96FFF1FF8E035A31E4FB7B8CFF" box="[1072,1265,1937,1961]" italics="true" pageId="7" pageNumber="270">Deviata multilineae</emphasis>
|
||
</taxonomicName>
|
||
1630 bp, 45.77%, OP537914.
|
||
</paragraph>
|
||
<caption id="DF00660CFFFEFF8107EB328CFA3C8D59" ID-DOI="http://doi.org/10.5281/zenodo.8326410" ID-Zenodo-Dep="8326410" httpUri="https://zenodo.org/record/8326410/files/figure.png" pageId="8" pageNumber="271" startId="8.[129,194,1274,1298]" targetBox="[169,1433,144,1247]" targetPageId="8" targetType="figure">
|
||
<paragraph id="8BC03684FFFEFF8107EB328CFA3C8D59" blockId="8.[129,1474,1274,1551]" pageId="8" pageNumber="271">
|
||
<emphasis id="B90BEA96FFFEFF8107EB328CFF528E45" bold="true" box="[129,216,1274,1299]" pageId="8" pageNumber="271">Figure 5.</emphasis>
|
||
Morphogenetic process of
|
||
<taxonomicName id="4C7F4D07FFFEFF8106B3328DFD058E44" authorityName="Song & Dong & Lu & Al-Farraj & Song & Hines & Luo" authorityYear="2023" box="[473,655,1275,1299]" genus="Heterodeviata" pageId="8" pageNumber="271" rank="species" species="sinica">
|
||
<emphasis id="B90BEA96FFFEFF8106B3328DFD058E44" box="[473,655,1275,1299]" italics="true" pageId="8" pageNumber="271">Heterodeviata sinica</emphasis>
|
||
</taxonomicName>
|
||
asser protargol impregnation. A, showing the oral primordium of the opisthe. B, ventral view of a very early divider, showing oral primordium of the opisthe (arrowhead). C–E, ventro-lateral (C, D) and dorsolateral (E) views of the same early divider, in this stage, anlagen I–IV (C), two right marginal anlagen (E) formed, adoral zone of membranelles incompletely formed (arrowhead in C), and arrowhead in (D) shows the parental endoral beginning to disintegrate. F, G, ventral (F) and dorsal (G) views of two middle dividers, arrowheads in (G) showing dorsal kinety anlage 2. H, I, ventral (H) and dorsal view (I) of two middle dividers, in this stage, anlagen I–IV dedifferentiating into cirri (H), and two bristles of dorsal kinety 2 formed (arrowheads in H, I). J, dorsal view of a late divider, showing one macronuclear nodule dedifferentiated into two ones. K, L, ventral views of two late dividers, arrowheads in (K) and (L) indicate the buccal and parabuccal cirrus, separately. I–IV, anlagen I–IV; DK1, dorsal kinety 1; DḎ1, dorsal kinety anlage 1; FVR, frontoventral cirral row; LMA1, 2, less marginal anlagen 1, 2; LMR1, 2, less marginal rows 1, 2; Ma, macronuclear nodules; Mi, micronuclei; OP, oral primordium; RMA1, 2, right marginal anlagen 1, 2; RMR1, 2, right marginal rows 1, 2; UM, undulating membranes. Scale bars: 50 μm (F, L), 20 μm (G, I).
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BC03684FFFEFF8107F63048FE568CF9" blockId="8.[129,780,1598,1968]" pageId="8" pageNumber="271">
|
||
Phylogenetic trees based on 18S rRNA gene sequence data using BIandMLanalysesaremostlycongruent, therefore,onlytheMLtree isshownwithsupportsfrombothalgorithms (
|
||
<figureCitation id="13442A01FFFEFF81055D300BFDF08DC3" box="[567,634,1661,1685]" captionStart="Figure 2" captionStartId="3.[113,178,1044,1068]" captionTargetBox="[154,1415,146,1016]" captionTargetId="figure-446@3.[154,1418,143,1016]" captionTargetPageId="3" captionText="Figure 2. A, maximum likelihood tree inferred from 18S rRNA gene sequences, showing the phylogenetic positions of the two newly sequenced species. Numbers near the nodes represent the ML bootstrap support and BI posterior probability values. Fully supported (100%/1.00) branches are marked with solid circles. ‘Asterisks’ indicate disagreement between the ML and BI trees.Sequences newly obtained are in pink. The scale bar corresponds to one substitution per 100 nucleotide positions. B, the tree is made referring to the ML tree in a radiation view, showing the likely systematic relationship of Heterodeviata with related genera. C, topology of species within Deviatidae in Bayesian inference (BI) tree.‘**’ indicates the sequence Perisincirra sp. (KY855575) is probably misidentified, which should be conspecific with Deviata brasiliensis." figureDoi="http://doi.org/10.5281/zenodo.8326404" httpUri="https://zenodo.org/record/8326404/files/figure.png" pageId="8" pageNumber="271">Fig.2A</figureCitation>
|
||
). Accordingto the 18S rRNA gene tree, all deviatids group together in a large clade with high to full support (97%ML/1.00 BI), in which
|
||
<taxonomicName id="4C7F4D07FFFEFF8105E330CAFF3E8DA5" authorityName="Song & Dong & Lu & Al-Farraj & Song & Hines & Luo" authorityYear="2023" genus="Heterodeviata" pageId="8" pageNumber="271" rank="species" species="sinica">
|
||
<emphasis id="B90BEA96FFFEFF8105E330CAFF3E8DA5" italics="true" pageId="8" pageNumber="271">Heterodeviata sinica</emphasis>
|
||
</taxonomicName>
|
||
is placed in a subclade including
|
||
<taxonomicName id="4C7F4D07FFFEFF81069B30AAFD2E8DA5" box="[497,676,1755,1779]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="8" pageNumber="271" phylum="Ciliophora" rank="species" species="parabacilliformis">
|
||
<emphasis id="B90BEA96FFFEFF81069B30AAFD2E8DA5" box="[497,676,1755,1779]" italics="true" pageId="8" pageNumber="271">D. parabacilliformis</emphasis>
|
||
</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C7F4D07FFFEFF8105C430AAFC8E8DA5" box="[686,772,1756,1779]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="8" pageNumber="271" phylum="Ciliophora" rank="species" species="rositae">
|
||
<emphasis id="B90BEA96FFFEFF8105C430AAFC8E8DA5" box="[686,772,1756,1779]" italics="true" pageId="8" pageNumber="271">D. rositae</emphasis>
|
||
</taxonomicName>
|
||
, and
|
||
<taxonomicName id="4C7F4D07FFFEFF8107C1308CFE158C44" box="[171,415,1786,1810]" genus="Pseudosincirra" pageId="8" pageNumber="271" rank="species" species="longicirrata">
|
||
<emphasis id="B90BEA96FFFEFF8107C1308CFE158C44" box="[171,415,1786,1810]" italics="true" pageId="8" pageNumber="271">Pseudosincirra longicirrata</emphasis>
|
||
</taxonomicName>
|
||
with low support (69%ML/0.54BI). However, the position of
|
||
<taxonomicName id="4C7F4D07FFFEFF81061C316CFE4D8C67" authorityName="Song & Dong & Lu & Al-Farraj & Song & Hines & Luo" authorityYear="2023" box="[374,455,1818,1841]" genus="Heterodeviata" pageId="8" pageNumber="271" rank="species" species="sinica">
|
||
<emphasis id="B90BEA96FFFEFF81061C316CFE4D8C67" box="[374,455,1818,1841]" italics="true" pageId="8" pageNumber="271">H. sinica</emphasis>
|
||
</taxonomicName>
|
||
is not robust due to the low support for the cluster of
|
||
<taxonomicName id="4C7F4D07FFFEFF81063F314CFE2C8C07" authorityName="Song & Dong & Lu & Al-Farraj & Song & Hines & Luo" authorityYear="2023" box="[341,422,1850,1873]" genus="Heterodeviata" pageId="8" pageNumber="271" rank="species" species="sinica">
|
||
<emphasis id="B90BEA96FFFEFF81063F314CFE2C8C07" box="[341,422,1850,1873]" italics="true" pageId="8" pageNumber="271">H. sinica</emphasis>
|
||
</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C7F4D07FFFEFF8106DB314CFDEE8C07" box="[433,612,1849,1873]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="8" pageNumber="271" phylum="Ciliophora" rank="species" species="parabacilliformis">
|
||
<emphasis id="B90BEA96FFFEFF8106DB314CFDEE8C07" box="[433,612,1849,1873]" italics="true" pageId="8" pageNumber="271">D. parabacilliformis</emphasis>
|
||
</taxonomicName>
|
||
, and
|
||
<taxonomicName id="4C7F4D07FFFEFF8105F0314CFD7A8C07" box="[666,752,1850,1873]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="8" pageNumber="271" phylum="Ciliophora" rank="species" species="rositae">
|
||
<emphasis id="B90BEA96FFFEFF8105F0314CFD7A8C07" box="[666,752,1850,1873]" italics="true" pageId="8" pageNumber="271">D. rositae</emphasis>
|
||
</taxonomicName>
|
||
in the ML tree (50%) and the incongruent topology in the BI tree (
|
||
<figureCitation id="13442A01FFFEFF81058C312EFF148CC6" captionStart="Figure 2" captionStartId="3.[113,178,1044,1068]" captionTargetBox="[154,1415,146,1016]" captionTargetId="figure-446@3.[154,1418,143,1016]" captionTargetPageId="3" captionText="Figure 2. A, maximum likelihood tree inferred from 18S rRNA gene sequences, showing the phylogenetic positions of the two newly sequenced species. Numbers near the nodes represent the ML bootstrap support and BI posterior probability values. Fully supported (100%/1.00) branches are marked with solid circles. ‘Asterisks’ indicate disagreement between the ML and BI trees.Sequences newly obtained are in pink. The scale bar corresponds to one substitution per 100 nucleotide positions. B, the tree is made referring to the ML tree in a radiation view, showing the likely systematic relationship of Heterodeviata with related genera. C, topology of species within Deviatidae in Bayesian inference (BI) tree.‘**’ indicates the sequence Perisincirra sp. (KY855575) is probably misidentified, which should be conspecific with Deviata brasiliensis." figureDoi="http://doi.org/10.5281/zenodo.8326404" httpUri="https://zenodo.org/record/8326404/files/figure.png" pageId="8" pageNumber="271">Fig. 2C</figureCitation>
|
||
). Furthermore, the systematic relationship of
|
||
<taxonomicName id="4C7F4D07FFFEFF810532310EFD528CC6" authorityName="Song & Dong & Lu & Al-Farraj & Song & Hines & Luo" authorityYear="2023" box="[600,728,1912,1936]" genus="Heterodeviata" pageId="8" pageNumber="271" rank="genus">
|
||
<emphasis id="B90BEA96FFFEFF810532310EFD528CC6" box="[600,728,1912,1936]" italics="true" pageId="8" pageNumber="271">Heterodeviata</emphasis>
|
||
</taxonomicName>
|
||
with related genera is shown in
|
||
<figureCitation id="13442A01FFFEFF81061331E1FE5C8CF9" box="[377,470,1943,1967]" captionStart="Figure 2" captionStartId="3.[113,178,1044,1068]" captionTargetBox="[154,1415,146,1016]" captionTargetId="figure-446@3.[154,1418,143,1016]" captionTargetPageId="3" captionText="Figure 2. A, maximum likelihood tree inferred from 18S rRNA gene sequences, showing the phylogenetic positions of the two newly sequenced species. Numbers near the nodes represent the ML bootstrap support and BI posterior probability values. Fully supported (100%/1.00) branches are marked with solid circles. ‘Asterisks’ indicate disagreement between the ML and BI trees.Sequences newly obtained are in pink. The scale bar corresponds to one substitution per 100 nucleotide positions. B, the tree is made referring to the ML tree in a radiation view, showing the likely systematic relationship of Heterodeviata with related genera. C, topology of species within Deviatidae in Bayesian inference (BI) tree.‘**’ indicates the sequence Perisincirra sp. (KY855575) is probably misidentified, which should be conspecific with Deviata brasiliensis." figureDoi="http://doi.org/10.5281/zenodo.8326404" httpUri="https://zenodo.org/record/8326404/files/figure.png" pageId="8" pageNumber="271">Figure 2B</figureCitation>
|
||
.
|
||
</paragraph>
|
||
<paragraph id="8BC03684FFFEFF81043F3048FC1D8CF8" blockId="8.[825,1474,1598,1967]" pageId="8" pageNumber="271">
|
||
Comparing the two new 18S rRNA sequences with seven available deviatid sequences (
|
||
<figureCitation id="13442A01FFFEFF81031F3028FB3B8D20" box="[1141,1201,1630,1654]" captionStart="Figure 8" captionStartId="11.[113,178,996,1020]" captionTargetBox="[155,1418,144,969]" captionTargetId="figure-362@11.[154,1418,144,969]" captionTargetPageId="11" captionText="Figure 8. Nucleotide differences between deviatids based on 18S rRNA gene sequences.In (A), the lower less values of the table indicate the sequence similarity, the upper right numbers are numbers of nucleotide differences.The numbers in the header (B) indicate the unmatched site positions. In A, B, the newly obtained sequences are in red.‘**’ indicates the sequence Perisincirra sp. (KY855575) which was likely misidentified and can be considered conspecific with Deviata brasiliensis. ‘H’,Heterodeviata." figureDoi="http://doi.org/10.5281/zenodo.8326416" httpUri="https://zenodo.org/record/8326416/files/figure.png" pageId="8" pageNumber="271">Fig. 8</figureCitation>
|
||
),
|
||
<taxonomicName id="4C7F4D07FFFEFF8103A33028FA1C8D23" authorityName="Song & Dong & Lu & Al-Farraj & Song & Hines & Luo" authorityYear="2023" box="[1225,1430,1630,1654]" genus="Heterodeviata" pageId="8" pageNumber="271" rank="species" species="sinica">
|
||
<emphasis id="B90BEA96FFFEFF8103A33028FA1C8D23" box="[1225,1430,1630,1654]" italics="true" pageId="8" pageNumber="271">Heterodeviata sinica</emphasis>
|
||
</taxonomicName>
|
||
differs from the other deviatid sequences by 31–38 nucleotide positions (corresponding to 97.5–97.9% similarity). It is noteworthy that for the sequence of
|
||
<taxonomicName id="4C7F4D07FFFEFF8103F730CAFABD8D85" box="[1181,1335,1724,1747]" class="Hypotrichea" family="Oxytrichidae" genus="Perisincirra" kingdom="Chromista" order="Oxytrichida" pageId="8" pageNumber="271" phylum="Ciliophora" rank="species" species="undetermined">
|
||
<emphasis id="B90BEA96FFFEFF8103F730CAFA9B8D85" box="[1181,1297,1724,1747]" italics="true" pageId="8" pageNumber="271">Perisincirra</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
(KY855575, submiưed by Nitla from Pisa University), the morphological characterization was unavailable, but there were no nucleotides differences from
|
||
<taxonomicName id="4C7F4D07FFFEFF81034F316CFB638C64" box="[1061,1257,1818,1842]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="8" pageNumber="271" phylum="Ciliophora" rank="species" species="brasiliensis">
|
||
<emphasis id="B90BEA96FFFEFF81034F316CFB638C64" box="[1061,1257,1818,1842]" italics="true" pageId="8" pageNumber="271">Deviata brasiliensis</emphasis>
|
||
</taxonomicName>
|
||
, which suggests that
|
||
<taxonomicName id="4C7F4D07FFFEFF810453314CFC598C06" box="[825,979,1849,1873]" class="Hypotrichea" family="Oxytrichidae" genus="Perisincirra" kingdom="Chromista" order="Oxytrichida" pageId="8" pageNumber="271" phylum="Ciliophora" rank="species" species="undetermined">
|
||
<emphasis id="B90BEA96FFFEFF810453314CFC278C07" box="[825,941,1850,1873]" italics="true" pageId="8" pageNumber="271">Perisincirra</emphasis>
|
||
sp.
|
||
</taxonomicName>
|
||
(KY855575) was misidentified, and probably is conspecific with
|
||
<taxonomicName id="4C7F4D07FFFEFF810495312FFB488C26" box="[1023,1218,1880,1904]" class="Hypotrichea" family="Kahliellidae" genus="Deviata" kingdom="Chromista" order="Urostylida" pageId="8" pageNumber="271" phylum="Ciliophora" rank="species" species="brasiliensis">
|
||
<emphasis id="B90BEA96FFFEFF810495312FFB488C26" box="[1023,1218,1880,1904]" italics="true" pageId="8" pageNumber="271">Deviata brasiliensis</emphasis>
|
||
</taxonomicName>
|
||
. More detailed information about the nine deviatid sequences are presented within
|
||
<figureCitation id="13442A01FFFEFF81045331E1FC1A8CF9" box="[825,912,1943,1967]" captionStart="Figure 8" captionStartId="11.[113,178,996,1020]" captionTargetBox="[155,1418,144,969]" captionTargetId="figure-362@11.[154,1418,144,969]" captionTargetPageId="11" captionText="Figure 8. Nucleotide differences between deviatids based on 18S rRNA gene sequences.In (A), the lower less values of the table indicate the sequence similarity, the upper right numbers are numbers of nucleotide differences.The numbers in the header (B) indicate the unmatched site positions. In A, B, the newly obtained sequences are in red.‘**’ indicates the sequence Perisincirra sp. (KY855575) which was likely misidentified and can be considered conspecific with Deviata brasiliensis. ‘H’,Heterodeviata." figureDoi="http://doi.org/10.5281/zenodo.8326416" httpUri="https://zenodo.org/record/8326416/files/figure.png" pageId="8" pageNumber="271">Figure 8</figureCitation>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |