treatments-xml/data/03/BE/35/03BE357FFF901678FF2D44BAFDE7B09F.xml

<document id="427668B6817E0CE4304A90A4176FBFBD" ID-CLB-Dataset="124036" ID-DOI="10.11646/phytotaxa.558.2.3" ID-GBIF-Dataset="79526b70-f091-4497-b12e-f79c81ca9c25" ID-ISSN="1179-3163" ID-Zenodo-Dep="7002943" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1660732614044" checkinUser="plazi" docAuthor="Lestari, Anis S. &amp; Thilini Chethana, K. W." docDate="2022" docId="03BE357FFF901678FF2D44BAFDE7B09F" docLanguage="en" docName="phytotaxa.558.2.3.pdf" docOrigin="Phytotaxa 558 (2)" docSource="http://dx.doi.org/10.11646/phytotaxa.558.2.3" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.7:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="7" docTitle="Calycina montana Lestari &amp; Thilini Chethana 2022, comb. nov." docType="treatment" docVersion="7" lastPageNumber="193" masterDocId="FF874D07FF951670FFA5445CFFEFB24B" masterDocTitle="Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales)" masterLastPageNumber="202" masterPageNumber="185" pageNumber="190" updateTime="1699367151215" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="76F39F47CD2AE02A4B69D8DA14FF931C">Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales)</mods:title>
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<mods:namePart id="1DCE910FA7A826A5797504BE881E7D4D">Lestari, Anis S.</mods:namePart>
<mods:nameIdentifier id="C76571DED8B2042E6D5AC825A740CC0A" type="ORCID">0000-0002-1606-9884</mods:nameIdentifier>
<mods:affiliation id="32A1B164AAA627EACFBFEA8B1F44DC3D">School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Research Organization for Life Sciences, National Research and Innovation Agency (BRIN), Cibinong 16911, Indonesia &amp; anislestari 1 @ gmail. com; https: // orcid. org / 0000 - 0002 - 1606 - 9884</mods:affiliation>
<mods:nameIdentifier id="8F96CADA9C667B1A08F99E4A52A08B4F" type="email">anislestari1@gmail.com</mods:nameIdentifier>
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<mods:namePart id="29B4375453408FCC29BC39B1A09F4C5D">Thilini Chethana, K. W.</mods:namePart>
<mods:nameIdentifier id="66046D1FFCAC3F8DE44019EEF695714E" type="ORCID">0000-0002-5816-9269</mods:nameIdentifier>
<mods:affiliation id="5B6CF2D5A8A0CB3309B8991C2C9ED8AE">School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; tchethi @ yahoo. com; https: // orcid. org / 0000 - 0002 - 5816 - 9269</mods:affiliation>
<mods:nameIdentifier id="CDFC5F052EE2526D21E78D185483FE39" type="email">tchethi@yahoo.com</mods:nameIdentifier>
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<mods:date id="8A29F57247EE390D081A2EA824EC0564">2022</mods:date>
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<mods:number id="A57F4982C8AC0E57F717E9CD9FA2D47F">2022-08-15</mods:number>
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<mods:number id="7F744351DD20BDB2C7E9B92B35536699">558</mods:number>
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<treatment id="03BE357FFF901678FF2D44BAFDE7B09F" ID-DOI="http://doi.org/10.5281/zenodo.7007988" ID-GBIF-Taxon="199504785" ID-Zenodo-Dep="7007988" LSID="urn:lsid:plazi:treatment:03BE357FFF901678FF2D44BAFDE7B09F" httpUri="http://treatment.plazi.org/id/03BE357FFF901678FF2D44BAFDE7B09F" lastPageId="8" lastPageNumber="193" pageId="5" pageNumber="190">
<subSubSection id="C30DD7E2FF901675FF2D44BAFB1EB34A" box="[136,1265,230,257]" pageId="5" pageNumber="190" type="nomenclature">
<paragraph id="8BA88469FF901675FF2D44BAFB1EB34A" blockId="5.[136,1265,230,257]" box="[136,1265,230,257]" pageId="5" pageNumber="190">
<heading id="D0E03305FF901675FF2D44BAFB1EB34A" box="[136,1265,230,257]" fontSize="11" level="2" pageId="5" pageNumber="190" reason="3">
<taxonomicName id="4C17FFEAFF901675FF2D44BAFC78B34A" authority="(W. Y. Zhuang &amp; H. D. Zheng) Lestari &amp; Chethana" authorityName="Lestari &amp; Thilini Chethana" authorityYear="2022" baseAuthorityName="W. Y. Zhuang &amp; H. D. Zheng" baseAuthorityYear="2022" box="[136,919,230,257]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="5" pageNumber="190" phylum="Ascomycota" rank="species" species="montana" status="comb. nov.">
<emphasis id="B963587BFF901675FF2D44BAFEB2B34A" bold="true" box="[136,349,230,257]" italics="true" pageId="5" pageNumber="190">Calycina montana</emphasis>
(W.Y. Zhuang &amp; H.D. Zheng) Lestari &amp; Chethana
</taxonomicName>
, 
<emphasis id="B963587BFF901675FC0744BAFBF1B34A" bold="true" box="[930,1054,230,257]" italics="true" pageId="5" pageNumber="190">
<taxonomicNameLabel id="A250E500FF901675FC0744BAFBF1B34A" box="[930,1054,230,257]" pageId="5" pageNumber="190" rank="species">comb. nov.</taxonomicNameLabel>
</emphasis>
(
<figureCitation id="132C98ECFF901675FBEA44BBFB06B34A" box="[1103,1257,231,257]" captionStart-0="FIGURE 2" captionStart-1="FIGURE 3" captionStart-2="FIGURE 4" captionStartId-0="5.[136,229,1319,1341]" captionStartId-1="6.[136,229,1910,1932]" captionStartId-2="7.[136,229,1902,1924]" captionTargetBox-0="[153,1434,388,1288]" captionTargetBox-1="[205,1380,202,1874]" captionTargetBox-2="[204,1383,192,1871]" captionTargetId-0="figure-60@5.[151,1436,386,1291]" captionTargetId-1="figure-24@6.[183,1404,190,1885]" captionTargetId-2="figure-17@7.[183,1403,190,1878]" captionTargetPageId-0="5" captionTargetPageId-1="6" captionTargetPageId-2="7" captionText-0="FIGURE 2. Calycina montana (MFLU 22-0055, new geographical record).a Rotten wood substrates covered with mosses. b–c Apothecia on the substrate. d Close-up of an apothecium sectioning. e Close-up of hymenium at the margin. f Phialide cells on lower flank surface. g–h Conidiogenous cells bearing conidiospore i–j A conidiogenous cell (j Mounted in Congo red). Scale bars: b = 950 μm, c = 470 μm, d = 315 μm, e = 141 μm, f = 40 μm, g = 17 μm, h–j = 13 μm." captionText-1="FIGURE 3. Calycina montana (MFLU 22-0055, new geographical record). a Close up of excipulum cells. b Ectal excipulum cells (b mounted in Congo Red). c Medullary excipulum cells. d–e Filiform paraphyses with refractive guttules. f–g Asci (mounted in Congo red). h Asci (alive state). i Close up of crozier. j Tip of the ascus (J+, in Melzerr’s agent with KOH treatment). k–m Ascospores (m mounted in Congo red). Scale bars: a = 40 μm, b–c = 25 μm, d–h = 14 μm, i = 10 μm, j–m = 4 μm." captionText-2="FIGURE 4. Calycina montana (HMAS 275566, holotype). a A herbarium package. b An apothecium on the woody substrate. c–d Cross sections of an apothecium (d mounted in Congo red). e Close up of hymenium at the margin. f Close up of ectal excipulum cells. g–h Filiform paraphyses (dead state). i–l Asci (k mounted in Melzer’s agent, l mounted in Congo red). m Tip of the ascus (J+, in Melzer’s agent with KOH treatment). n Close up of crozier at the base of an immature ascus. o–p Ascospores (o mounted in Congo red). Scale bars: b = 500 μm, c–d = 380 μm, e = 43 μm, f = 25 μm, g–h = 23 μm, i–l = 19 μm, m = 4 μm, n = 8 μm, m, o–p = 4 μm." figureDoi-0="http://doi.org/10.5281/zenodo.7002947" figureDoi-1="http://doi.org/10.5281/zenodo.7002949" figureDoi-2="http://doi.org/10.5281/zenodo.7002953" httpUri-0="https://zenodo.org/record/7002947/files/figure.png" httpUri-1="https://zenodo.org/record/7002949/files/figure.png" httpUri-2="https://zenodo.org/record/7002953/files/figure.png" pageId="5" pageNumber="190">FIGURE 2–4</figureCitation>
)
</heading>
</paragraph>
</subSubSection>
<subSubSection id="C30DD7E2FF901675FF2D4552FCD9B30C" pageId="5" pageNumber="190" type="reference_group">
<paragraph id="8BA88469FF901675FF2D4552FB01B368" blockId="5.[136,1262,270,364]" box="[136,1262,270,292]" pageId="5" pageNumber="190">
Basionym: 
<treatmentCitation id="0AB6A278FF901675FF534552FB01B368" author="W. Y. Zhuang &amp; H. D. Zheng, Zhuang, Zheng &amp; Ren" box="[246,1262,270,292]" issue="4" journal="Mycosystema" page="407" pageId="5" pageNumber="190" score="1.97023359760351E-6" volume="36" year="2017">
<taxonomicName id="4C17FFEAFF901675FF534552FBD3B36F" ID-CoL="LX2J" authority="W. Y. Zhuang &amp; H. D. Zheng, Zhuang, Zheng &amp; Ren, Mycosystema" authorityName="W. Y. Zhuang &amp; H. D. Zheng, Zhuang, Zheng &amp; Ren, Mycosystema" box="[246,1084,270,292]" class="Leotiomycetes" family="Pezizellaceae" genus="Bisporella" kingdom="Fungi" order="Rhytismatales" pageId="5" pageNumber="190" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF901675FF534552FE5CB36F" box="[246,435,270,292]" italics="true" pageId="5" pageNumber="190">Bisporella montana</emphasis>
W.Y. Zhuang &amp; H.D. Zheng, Zhuang, Zheng &amp; Ren, Mycosystema
</taxonomicName>
36(4): 407 (2017)
</treatmentCitation>
</paragraph>
<paragraph id="8BA88469FF901675FF2D456EFCD9B30C" blockId="5.[136,1262,270,364]" box="[136,822,306,328]" pageId="5" pageNumber="190">Index Fungorum number: IF 570309, Facesoffungi number: FoF 10812</paragraph>
</subSubSection>
<subSubSection id="C30DD7E2FF901678FF2D450AFDE7B09F" lastPageId="8" lastPageNumber="193" pageId="5" pageNumber="190" type="materials_examined">
<paragraph id="8BA88469FF901675FF2D450AFE6EB327" blockId="5.[136,1262,270,364]" box="[136,385,342,364]" pageId="5" pageNumber="190">
<materialsCitation id="3B7F8E34FF901675FF2D450AFE6EB327" ID-GBIF-Occurrence="3890242304" box="[136,385,342,364]" collectionCode="HMAS" pageId="5" pageNumber="190" specimenCode="HMAS 275566" specimenCount="1" typeStatus="holotype">
<typeStatus id="54AC3ACBFF901675FF2D450AFF0AB327" box="[136,229,342,364]" pageId="5" pageNumber="190" type="holotype">Holotype</typeStatus>
: 
<specimenCode id="DBB12C12FF901675FF4B450AFE6EB327" box="[238,385,342,364]" collectionCode="HMAS" country="China" httpUri="http://grbio.org/cool/qht3-g0qs" name="Chinese Academy of Sciences" pageId="5" pageNumber="190" type="Herbarium">HMAS 275566</specimenCode>
</materialsCitation>
</paragraph>
<caption id="DF68D4E1FF901675FF2D417BFD2DB7E2" ID-DOI="http://doi.org/10.5281/zenodo.7002947" ID-Zenodo-Dep="7002947" httpUri="https://zenodo.org/record/7002947/files/figure.png" pageId="5" pageNumber="190" startId="5.[136,229,1319,1341]" targetBox="[153,1434,388,1288]" targetPageId="5">
<paragraph id="8BA88469FF901675FF2D417BFD2DB7E2" blockId="5.[136,1453,1319,1449]" pageId="5" pageNumber="190">
<emphasis id="B963587BFF901675FF2D417BFE5AB776" bold="true" box="[136,437,1319,1341]" pageId="5" pageNumber="190">
FIGURE 2. 
<taxonomicName id="4C17FFEAFF901675FEA5417BFE5AB776" authorityName="Lestari &amp; Thilini Chethana" authorityYear="2022" baseAuthorityName="W. Y. Zhuang &amp; H. D. Zheng" baseAuthorityYear="2022" box="[256,437,1319,1341]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="5" pageNumber="190" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF901675FEA5417BFE5AB776" bold="true" box="[256,437,1319,1341]" italics="true" pageId="5" pageNumber="190">Calycina montana</emphasis>
</taxonomicName>
</emphasis>
(MFLU 22-0055, new geographical record). 
<emphasis id="B963587BFF901675FCC54174FC83B776" bold="true" box="[864,876,1320,1341]" pageId="5" pageNumber="190">a</emphasis>
Rotten wood substrates covered with mosses. 
<emphasis id="B963587BFF901675FA874174FAAAB776" bold="true" box="[1314,1349,1320,1341]" pageId="5" pageNumber="190">b–c</emphasis>
Apothecia on the substrate. 
<emphasis id="B963587BFF901675FE8E4110FED7B72A" bold="true" box="[299,312,1356,1377]" pageId="5" pageNumber="190">d</emphasis>
Close-up of an apothecium sectioning. 
<emphasis id="B963587BFF901675FD1D4110FD2CB72A" bold="true" box="[696,707,1356,1377]" pageId="5" pageNumber="190">e</emphasis>
Close-up of hymenium at the margin. 
<emphasis id="B963587BFF901675FB9D4117FBAFB72A" bold="true" box="[1080,1088,1355,1377]" pageId="5" pageNumber="190">f</emphasis>
Phialide cells on lower flank surface. 
<emphasis id="B963587BFF901675FF2D412CFF42B7CE" bold="true" box="[136,173,1392,1413]" pageId="5" pageNumber="190">g–h</emphasis>
Conidiogenous cells bearing conidiospore 
<emphasis id="B963587BFF901675FDE94133FD89B7CE" bold="true" box="[588,614,1391,1413]" pageId="5" pageNumber="190">i–j</emphasis>
A conidiogenous cell (
<emphasis id="B963587BFF901675FCE64133FCA4B7CE" bold="true" box="[835,843,1391,1413]" pageId="5" pageNumber="190">j</emphasis>
Mounted in Congo red). Scale bars: b = 950 μm, c = 470 μm, d = 315 μm, e = 141 μm, f = 40 μm, g = 17 μm, h–j = 13 μm.
</paragraph>
</caption>
<paragraph id="8BA88469FF901675FF2D4184FDF6B5A1" blockId="5.[136,1452,1496,2026]" pageId="5" pageNumber="190">
<emphasis id="B963587BFF901675FF2D4184FF03B7B9" box="[136,236,1496,1522]" italics="true" pageId="5" pageNumber="190">Saprobic</emphasis>
on decayed woods covered with mosses. 
<emphasis id="B963587BFF901675FD114184FCB5B7B9" bold="true" box="[692,858,1496,1522]" pageId="5" pageNumber="190">Sexual morph</emphasis>
: 
<emphasis id="B963587BFF901675FCC24184FC36B7B9" box="[871,985,1496,1522]" italics="true" pageId="5" pageNumber="190">Apothecia</emphasis>
1.1–1.6 × 
<quantity id="4CEF298CFF901675FBE94184FB3CB7B9" box="[1100,1235,1496,1522]" metricMagnitude="-3" metricUnit="m" metricValue="1.0" metricValueMax="1.4" metricValueMin="0.6" pageId="5" pageNumber="190" unit="mm" value="1.0" valueMax="1.4" valueMin="0.6">0.6–1.4 mm</quantity>
, gregarious, arising singly or in clusters, amber colored, stipitate. 
<emphasis id="B963587BFF901675FD2541A0FD57B45D" box="[640,696,1532,1558]" italics="true" pageId="5" pageNumber="190">Stipe</emphasis>
0.6–1.2 x 
<quantity id="4CEF298CFF901675FC8941A0FC5DB45D" box="[812,946,1532,1558]" metricMagnitude="-4" metricUnit="m" metricValue="3" metricValueMax="4.0" metricValueMin="2.0" pageId="5" pageNumber="190" unit="mm" value="0.3" valueMax="0.4" valueMin="0.2">0.2–0.4 mm</quantity>
in dried condition. 
<emphasis id="B963587BFF901675FB2F41A0FAEAB45D" box="[1162,1285,1532,1558]" italics="true" pageId="5" pageNumber="190">Receptacle</emphasis>
discoid, bright amber color to hyaline. 
<emphasis id="B963587BFF901675FE2A427DFE0DB471" box="[399,482,1569,1594]" italics="true" pageId="5" pageNumber="190">Margin</emphasis>
hyaline with brown outer layer at the flank. 
<emphasis id="B963587BFF901675FC68427DFBEFB471" box="[973,1024,1569,1594]" italics="true" pageId="5" pageNumber="190">Disc</emphasis>
concave. 
<emphasis id="B963587BFF901675FBD5427CFACBB471" box="[1136,1316,1568,1594]" italics="true" pageId="5" pageNumber="190">Ectal excipulum</emphasis>
55–68 µm (= 59.8 µm, n = 10) at the flank near the stipe, composed of gelatinized thick-walled, hyaline cells of 
<emphasis id="B963587BFF901675FB554219FA4BB415" box="[1264,1444,1605,1630]" italics="true" pageId="5" pageNumber="190">textura porrecta</emphasis>
. 
<emphasis id="B963587BFF901675FF2D4234FE96B4C9" box="[136,377,1640,1666]" italics="true" pageId="5" pageNumber="190">Medullary excipulum</emphasis>
36–80 µm (= 57.7 µm, n = 10) at the flank near the stipe, distinctively separate from ectal excipulum, composed of thin-walled, hyaline cells of 
<emphasis id="B963587BFF901675FD4C42D1FC6DB4ED" box="[745,898,1676,1702]" italics="true" pageId="5" pageNumber="190">textura oblita</emphasis>
, hypha with refractive guttules. 
<emphasis id="B963587BFF901675FB5742D1FA84B4ED" box="[1266,1387,1677,1702]" italics="true" pageId="5" pageNumber="190">Hymenium</emphasis>
141– 180 µm (= 168.9 µm, n = 15), amber colored. 
<emphasis id="B963587BFF901675FD0142ECFCC8B481" box="[676,807,1712,1738]" italics="true" pageId="5" pageNumber="190">Paraphyses</emphasis>
1.8–2.5 µm in diam. (= 2.1 µm, n = 15) at the terminal, filiform, septate, branched at the 1/3 length from the base, guttules. 
<emphasis id="B963587BFF901675FCD34288FC49B4A5" box="[886,934,1748,1774]" italics="true" pageId="5" pageNumber="190">Asci</emphasis>
101–118 × 4.7–6.3 µm (= 108.6 × 5.5 µm, n = 15), unitunicate, 8-spored, cylindrical and subclavate at the top, rounded (in alive state) to conical apex (dead state), J+ (eu-amyloid, in Melzer’s with and without KOH treatment), long substipitate base, arising from croziers. 
<emphasis id="B963587BFF901675FA884340FA43B57D" box="[1325,1452,1820,1846]" italics="true" pageId="5" pageNumber="190">Ascospores</emphasis>
7.1–9.2 × 4.1–5 µm (= 8 × 4.4 µm, n = 20), ellipsoid, 0-1 septate, partially biseriate to uniseriate, hyaline, rounded at both ends with guttules. 
<emphasis id="B963587BFF901675FE3D4338FDA1B535" bold="true" box="[408,590,1892,1918]" pageId="5" pageNumber="190">Asexual morph</emphasis>
: 
<emphasis id="B963587BFF901675FDF94339FCEEB535" box="[604,769,1893,1918]" italics="true" pageId="5" pageNumber="190">Hypomycetous</emphasis>
, on the lower flank and receptacle of the apothecia’s surface. 
<emphasis id="B963587BFF901675FF2D43D4FE83B5E9" box="[136,364,1928,1954]" italics="true" pageId="5" pageNumber="190">Conidiogenous cells</emphasis>
12–19 × 3.7–5.1 (= 14.3 × 4.3 µm, n = 10) µm, phialidic, black to dark brown. 
<emphasis id="B963587BFF901675FAB443D4FA43B5E9" box="[1297,1452,1928,1954]" italics="true" pageId="5" pageNumber="190">Conidiophore</emphasis>
1.5–3.7 × 1.6–2.4 (= 3 × 1.9, n = 5) µm, black to dark brown. 
<taxonomicName id="4C17FFEAFF901675FCF543F0FC45B58D" box="[848,938,1964,1990]" class="Arthoniomycetes" family="Arthoniaceae" genus="Conidia" higherTaxonomySource="GBIF" kingdom="Fungi" order="Arthoniales" pageId="5" pageNumber="190" phylum="Ascomycota" rank="genus">
<emphasis id="B963587BFF901675FCF543F0FC45B58D" box="[848,938,1964,1990]" italics="true" pageId="5" pageNumber="190">Conidia</emphasis>
</taxonomicName>
3.2–3.8 × 1.7–2 (= 3.5 × 1.9 µm, n = 2) µm ellipsoid, aseptate, hyaline, smooth.
</paragraph>
<caption id="DF68D4E1FF931676FF2D432AFC24B5BC" ID-DOI="http://doi.org/10.5281/zenodo.7002949" ID-Zenodo-Dep="7002949" httpUri="https://zenodo.org/record/7002949/files/figure.png" pageId="6" pageNumber="191" startId="6.[136,229,1910,1932]" targetBox="[205,1380,202,1874]" targetPageId="6">
<paragraph id="8BA88469FF931676FF2D432AFC24B5BC" blockId="6.[136,1452,1909,2040]" pageId="6" pageNumber="191">
<emphasis id="B963587BFF931676FF2D432AFE52B5C0" bold="true" box="[136,445,1909,1932]" pageId="6" pageNumber="191">
FIGURE 3. 
<taxonomicName id="4C17FFEAFF931676FEA34329FE52B5C0" authorityName="Lestari &amp; Thilini Chethana" authorityYear="2022" baseAuthorityName="W. Y. Zhuang &amp; H. D. Zheng" baseAuthorityYear="2022" box="[262,445,1909,1931]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="6" pageNumber="191" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF931676FEA34329FE52B5C0" bold="true" box="[262,445,1909,1931]" italics="true" pageId="6" pageNumber="191">Calycina montana</emphasis>
</taxonomicName>
</emphasis>
(MFLU 22-0055, new geographical record). 
<emphasis id="B963587BFF931676FCDD432AFC6BB5C0" bold="true" box="[888,900,1910,1931]" pageId="6" pageNumber="191">a</emphasis>
Close up of excipulum cells. 
<emphasis id="B963587BFF931676FB0C432AFB59B5C0" bold="true" box="[1193,1206,1910,1931]" pageId="6" pageNumber="191">b</emphasis>
Ectal excipulum cells (
<emphasis id="B963587BFF931676FA3B432AFA44B5C0" bold="true" box="[1438,1451,1910,1931]" pageId="6" pageNumber="191">b</emphasis>
mounted in Congo Red). 
<emphasis id="B963587BFF931676FEDC43C6FE6BB5E4" bold="true" box="[377,388,1946,1967]" pageId="6" pageNumber="191">c</emphasis>
Medullary excipulum cells. 
<emphasis id="B963587BFF931676FD3143C6FD57B5E4" bold="true" box="[660,696,1946,1967]" pageId="6" pageNumber="191">d–e</emphasis>
Filiform paraphyses with refractive guttules. 
<emphasis id="B963587BFF931676FBCE43C6FB64B5FB" bold="true" box="[1131,1163,1946,1968]" pageId="6" pageNumber="191">f–g</emphasis>
Asci (mounted in Congo red). 
<emphasis id="B963587BFF931676FF2D43E2FF7AB598" bold="true" box="[136,149,1982,2003]" pageId="6" pageNumber="191">h</emphasis>
Asci (alive state). 
<emphasis id="B963587BFF931676FEED43E2FEA0B59F" bold="true" box="[328,335,1982,2004]" pageId="6" pageNumber="191">i</emphasis>
Close up of crozier. 
<emphasis id="B963587BFF931676FDB343E2FDF1B59F" bold="true" box="[534,542,1982,2004]" pageId="6" pageNumber="191">j</emphasis>
Tip of the ascus (J+, in Melzerr’s agent with KOH treatment). 
<emphasis id="B963587BFF931676FBDD43E2FB49B598" bold="true" box="[1144,1190,1982,2003]" pageId="6" pageNumber="191">k–m</emphasis>
Ascospores (m mounted in Congo red). Scale bars: a = 40 μm, b–c = 25 μm, d–h = 14 μm, i = 10 μm, j–m = 4 μm.
</paragraph>
</caption>
<caption id="DF68D4E1FF921677FF2D4332FB42BA58" ID-DOI="http://doi.org/10.5281/zenodo.7002953" ID-Zenodo-Dep="7002953" httpUri="https://zenodo.org/record/7002953/files/figure.png" pageId="7" pageNumber="192" startId="7.[136,229,1902,1924]" targetBox="[204,1383,192,1871]" targetPageId="7">
<paragraph id="8BA88469FF921677FF2D4332FB42BA58" blockId="7.[136,1452,1901,2068]" pageId="7" pageNumber="192">
<emphasis id="B963587BFF921677FF2D4332FE57B5C8" bold="true" box="[136,440,1901,1924]" pageId="7" pageNumber="192">
FIGURE 4. 
<taxonomicName id="4C17FFEAFF921677FEA64331FE57B5C8" authorityName="Lestari &amp; Thilini Chethana" authorityYear="2022" baseAuthorityName="W. Y. Zhuang &amp; H. D. Zheng" baseAuthorityYear="2022" box="[259,440,1901,1923]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="7" pageNumber="192" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF921677FEA64331FE57B5C8" bold="true" box="[259,440,1901,1923]" italics="true" pageId="7" pageNumber="192">Calycina montana</emphasis>
</taxonomicName>
</emphasis>
(HMAS 275566, 
<emphasis id="B963587BFF921677FDC14332FD53B5C8" bold="true" box="[612,700,1902,1923]" pageId="7" pageNumber="192">holotype</emphasis>
). 
<emphasis id="B963587BFF921677FD754332FD33B5C8" bold="true" box="[720,732,1902,1923]" pageId="7" pageNumber="192">a</emphasis>
A herbarium package. 
<emphasis id="B963587BFF921677FC1D4332FC2AB5C8" bold="true" box="[952,965,1902,1923]" pageId="7" pageNumber="192">b</emphasis>
An apothecium on the woody substrate. 
<emphasis id="B963587BFF921677FAEE4332FA80B5C8" bold="true" box="[1355,1391,1902,1923]" pageId="7" pageNumber="192">c–d</emphasis>
Cross sections of an apothecium (
<emphasis id="B963587BFF921677FE3343CEFE4CB5EC" bold="true" box="[406,419,1938,1959]" pageId="7" pageNumber="192">d</emphasis>
mounted in Congo red). 
<emphasis id="B963587BFF921677FD3E43CEFD49B5EC" bold="true" box="[667,678,1938,1959]" pageId="7" pageNumber="192">e</emphasis>
Close up of hymenium at the margin. 
<emphasis id="B963587BFF921677FB8643CEFBC4B5E3" bold="true" box="[1059,1067,1938,1960]" pageId="7" pageNumber="192">f</emphasis>
Close up of ectal excipulum cells. 
<emphasis id="B963587BFF921677FA2343CEFA44B5EC" bold="true" box="[1414,1451,1938,1959]" pageId="7" pageNumber="192">g–h</emphasis>
Filiform paraphyses (dead state). 
<emphasis id="B963587BFF921677FE6343EAFE30B587" bold="true" box="[454,479,1974,1996]" pageId="7" pageNumber="192">i–l</emphasis>
Asci (
<emphasis id="B963587BFF921677FDB943EAFDC6B580" bold="true" box="[540,553,1974,1995]" pageId="7" pageNumber="192">k</emphasis>
mounted in Melzer’s agent, 
<emphasis id="B963587BFF921677FC9243EAFCD1B580" bold="true" box="[823,830,1974,1995]" pageId="7" pageNumber="192">l</emphasis>
mounted in Congo red). 
<emphasis id="B963587BFF921677FB8F43EAFBD1B580" bold="true" box="[1066,1086,1974,1995]" pageId="7" pageNumber="192">m</emphasis>
Tip of the ascus (J+, in Melzer’s agent with KOH treatment). 
<emphasis id="B963587BFF921677FEC74386FE80B5A4" bold="true" box="[354,367,2010,2031]" pageId="7" pageNumber="192">n</emphasis>
Close up of crozier at the base of an immature ascus. 
<emphasis id="B963587BFF921677FCDD4386FC72B5A4" bold="true" box="[888,925,2010,2031]" pageId="7" pageNumber="192">o–p</emphasis>
Ascospores (
<emphasis id="B963587BFF921677FBBA4386FBC4B5A4" bold="true" box="[1055,1067,2010,2031]" pageId="7" pageNumber="192">o</emphasis>
mounted in Congo red). Scale bars: b = 500 μm, c–d = 380 μm, e = 43 μm, f = 25 μm, g–h = 23 μm, i–l = 19 μm, m = 4 μm, n = 8 μm, m, o–p = 4 μm.
</paragraph>
</caption>
<paragraph id="8BA88469FF9D1678FF1844C2FDD5B36F" blockId="8.[136,1452,158,725]" pageId="8" pageNumber="193">
<materialsCitation id="3B7F8E34FF9D1678FF1844C2FA43B297" ID-GBIF-Occurrence="3890242301" collectingDate="1999-09-05" collectingDateMax="2021-10-20" collectingDateMin="1999-09-05" collectionCode="MFLU, HMAS" collectorName="Anis S. Lestari" country="China" county="Material" location="Chiang Mai Province" municipality="Thailand" pageId="8" pageNumber="193" specimenCode="MFLU 22-0055, MFLU 22-0056, HMAS 275566" specimenCount="1" stateProvince="Yunnan" typeStatus="holotype">
<collectingCounty id="62C9FCE5FF9D1678FF1844C2FEF3B2F3" box="[189,284,158,184]" pageId="8" pageNumber="193">Material</collectingCounty>
examined: 
<collectingMunicipality id="6BCC1E13FF9D1678FE0144C2FDE5B2F3" box="[420,522,158,184]" pageId="8" pageNumber="193">Thailand</collectingMunicipality>
, 
<location id="8EC8D2B2FF9D1678FDBD44C2FCE0B2F3" LSID="urn:lsid:plazi:treatment:03BE357FFF901678FF2D44BAFDE7B09F:8EC8D2B2FF9D1678FDBD44C2FCE0B2F3" box="[536,783,158,184]" country="China" county="Material" municipality="Thailand" name="Chiang Mai Province" pageId="8" pageNumber="193" stateProvince="Yunnan">Chiang Mai Province</location>
, 
<location id="8EC8D2B2FF9D1678FCB844C2FBEDB2F3" LSID="urn:lsid:plazi:treatment:03BE357FFF901678FF2D44BAFDE7B09F:8EC8D2B2FF9D1678FCB844C2FBEDB2F3" box="[797,1026,158,184]" country="China" county="Material" municipality="Thailand" name="Chomthong District" pageId="8" pageNumber="193" stateProvince="Yunnan">Chomthong District</location>
, 
<location id="8EC8D2B2FF9D1678FBB544C2FB45B2F3" LSID="urn:lsid:plazi:treatment:03BE357FFF901678FF2D44BAFDE7B09F:8EC8D2B2FF9D1678FBB544C2FB45B2F3" box="[1040,1194,158,184]" country="China" county="Material" municipality="Thailand" name="Doi Inthanon" pageId="8" pageNumber="193" stateProvince="Yunnan">Doi Inthanon</location>
, 
<location id="8EC8D2B2FF9D1678FB1D44C3FAB3B2F2" LSID="urn:lsid:plazi:treatment:03BE357FFF901678FF2D44BAFDE7B09F:8EC8D2B2FF9D1678FB1D44C3FAB3B2F2" box="[1208,1372,159,185]" country="China" county="Material" municipality="Thailand" name="Kew Mae Pan" pageId="8" pageNumber="193" stateProvince="Yunnan">Kew Mae Pan</location>
nature trail, 
<date id="FFA9A2A9FF9D1678FF63449EFE63B297" box="[198,396,194,220]" pageId="8" pageNumber="193" value="2021-10-20">
<collectingDate id="EFED5B41FF9D1678FF63449EFE63B297" box="[198,396,194,220]" pageId="8" pageNumber="193" value="2021-10-20">20 October 2021</collectingDate>
</date>
, 
<collectorName id="26E2E1BFFF9D1678FE3D449EFDA6B297" box="[408,585,194,220]" pageId="8" pageNumber="193">Anis S. Lestari</collectorName>
, KMP3 21 (
<specimenCode id="DBB12C12FF9D1678FD7C449EFC7BB296" box="[729,916,194,221]" collectionCode="MFLU" country="Thailand" httpUri="http://grbio.org/cool/672j-mk16" name="Mae Fah Laung University Herbarium" pageId="8" pageNumber="193">MFLU 22-0055</specimenCode>
), KMP3 41 (
<specimenCode id="DBB12C12FF9D1678FB88449EFB09B296" box="[1069,1254,194,221]" collectionCode="MFLU" country="Thailand" httpUri="http://grbio.org/cool/672j-mk16" name="Mae Fah Laung University Herbarium" pageId="8" pageNumber="193">MFLU 22-0056</specimenCode>
) (
<figureCitation id="132C98ECFF9D1678FB5A449EFA8DB297" box="[1279,1378,194,220]" captionStart="FIGURE 2" captionStartId="5.[136,229,1319,1341]" captionTargetBox="[153,1434,388,1288]" captionTargetId="figure-60@5.[151,1436,386,1291]" captionTargetPageId="5" captionText="FIGURE 2. Calycina montana (MFLU 22-0055, new geographical record).a Rotten wood substrates covered with mosses. b–c Apothecia on the substrate. d Close-up of an apothecium sectioning. e Close-up of hymenium at the margin. f Phialide cells on lower flank surface. g–h Conidiogenous cells bearing conidiospore i–j A conidiogenous cell (j Mounted in Congo red). Scale bars: b = 950 μm, c = 470 μm, d = 315 μm, e = 141 μm, f = 40 μm, g = 17 μm, h–j = 13 μm." figureDoi="http://doi.org/10.5281/zenodo.7002947" httpUri="https://zenodo.org/record/7002947/files/figure.png" pageId="8" pageNumber="193">Figure 2</figureCitation>
&amp; 
<figureCitation id="132C98ECFF9D1678FA29449EFA74B297" box="[1420,1435,194,220]" captionStart="FIGURE 3" captionStartId="6.[136,229,1910,1932]" captionTargetBox="[205,1380,202,1874]" captionTargetId="figure-24@6.[183,1404,190,1885]" captionTargetPageId="6" captionText="FIGURE 3. Calycina montana (MFLU 22-0055, new geographical record). a Close up of excipulum cells. b Ectal excipulum cells (b mounted in Congo Red). c Medullary excipulum cells. d–e Filiform paraphyses with refractive guttules. f–g Asci (mounted in Congo red). h Asci (alive state). i Close up of crozier. j Tip of the ascus (J+, in Melzerr’s agent with KOH treatment). k–m Ascospores (m mounted in Congo red). Scale bars: a = 40 μm, b–c = 25 μm, d–h = 14 μm, i = 10 μm, j–m = 4 μm." figureDoi="http://doi.org/10.5281/zenodo.7002949" httpUri="https://zenodo.org/record/7002949/files/figure.png" pageId="8" pageNumber="193">3</figureCitation>
);
</materialsCitation>
<materialsCitation id="3B7F8E34FF9D1678FF2D44BAFDD9B36F" ID-GBIF-Occurrence="3890242303" collectingDate="1999-09-05" collectingDateMax="2021-10-20" collectingDateMin="1999-09-05" collectionCode="MFLU, HMAS" collectorName="X. Q. Zhang &amp; D. Z. Ren" country="China" county="Material" location="Chiang Mai Province" municipality="Thailand" pageId="8" pageNumber="193" specimenCode="MFLU 22-0055, MFLU 22-0056, HMAS 275566" specimenCount="1" stateProvince="Yunnan" typeStatus="holotype">
<collectingCountry id="F300C4F9FF9D1678FF2D44BAFF0BB34B" box="[136,228,230,256]" name="China" pageId="8" pageNumber="193">CHINA</collectingCountry>
, 
<collectingRegion id="49D34A8BFF9D1678FF4B44BBFEA8B34A" box="[238,327,231,257]" country="China" name="Yunnan" pageId="8" pageNumber="193">Yunnan</collectingRegion>
, Shangrila, Pingbian, Daweishan on rotten hard wood, 
<date id="FFA9A2A9FF9D1678FC1344BAFB69B34B" box="[950,1158,230,257]" pageId="8" pageNumber="193" value="1999-09-05">
<collectingDate id="EFED5B41FF9D1678FC1344BAFB69B34B" box="[950,1158,230,257]" pageId="8" pageNumber="193" value="1999-09-05">5 September 1999</collectingDate>
</date>
, 
<collectorName id="26E2E1BFFF9D1678FB3444BAFAF7B34B" box="[1169,1304,230,256]" pageId="8" pageNumber="193">X.Q. Zhang</collectorName>
&amp; 
<collectorName id="26E2E1BFFF9D1678FA9944BBFA48B34A" box="[1340,1447,231,257]" pageId="8" pageNumber="193">D.Z. Ren</collectorName>
, (
<specimenCode id="DBB12C12FF9D1678FF354556FEAFB36E" box="[144,320,266,293]" collectionCode="HMAS" country="China" httpUri="http://grbio.org/cool/qht3-g0qs" name="Chinese Academy of Sciences" pageId="8" pageNumber="193" type="Herbarium">HMAS 275566</specimenCode>
, 
<emphasis id="B963587BFF9D1678FEEE4556FE5DB36F" bold="true" box="[331,434,266,292]" pageId="8" pageNumber="193">
<typeStatus id="54AC3ACBFF9D1678FEEE4556FE5DB36F" box="[331,434,266,292]" pageId="8" pageNumber="193" type="holotype">holotype</typeStatus>
</emphasis>
) (
<figureCitation id="132C98ECFF9D1678FE6F4556FDC2B36F" box="[458,557,266,292]" captionStart="FIGURE 4" captionStartId="7.[136,229,1902,1924]" captionTargetBox="[204,1383,192,1871]" captionTargetId="figure-17@7.[183,1403,190,1878]" captionTargetPageId="7" captionText="FIGURE 4. Calycina montana (HMAS 275566, holotype). a A herbarium package. b An apothecium on the woody substrate. c–d Cross sections of an apothecium (d mounted in Congo red). e Close up of hymenium at the margin. f Close up of ectal excipulum cells. g–h Filiform paraphyses (dead state). i–l Asci (k mounted in Melzer’s agent, l mounted in Congo red). m Tip of the ascus (J+, in Melzer’s agent with KOH treatment). n Close up of crozier at the base of an immature ascus. o–p Ascospores (o mounted in Congo red). Scale bars: b = 500 μm, c–d = 380 μm, e = 43 μm, f = 25 μm, g–h = 23 μm, i–l = 19 μm, m = 4 μm, n = 8 μm, m, o–p = 4 μm." figureDoi="http://doi.org/10.5281/zenodo.7002953" httpUri="https://zenodo.org/record/7002953/files/figure.png" pageId="8" pageNumber="193">Figure 4</figureCitation>
)
</materialsCitation>
.
</paragraph>
<paragraph id="8BA88469FF9D1678FF184572FEEAB326" blockId="8.[136,1452,158,725]" pageId="8" pageNumber="193">
GenBank numbers: 
<collectionCode id="ED061CACFF9D1678FE3E4573FE06B302" box="[411,489,303,329]" country="Thailand" httpUri="http://grbio.org/cool/672j-mk16" name="Mae Fah Laung University Herbarium" pageId="8" pageNumber="193">MFLU</collectionCode>
22-0055: 
<collectionCode id="ED061CACFF9D1678FDFF4572FD6BB302" box="[602,644,302,329]" pageId="8" pageNumber="193">ITS</collectionCode>
– 
<accessionNumber id="9444198AFF9D1678FD374572FCFEB302" box="[658,785,302,329]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/ON156524" pageId="8" pageNumber="193">ON156524</accessionNumber>
, 
<collectionCode id="ED061CACFF9D1678FCBE4572FCBEB302" box="[795,849,302,329]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15590" lsid="urn:lsid:biocol.org:col:15590" name="Louisiana State University - Herbarium" pageId="8" pageNumber="193" type="Herbarium">LSU</collectionCode>
– 
<accessionNumber id="9444198AFF9D1678FCC44572FC31B302" box="[865,990,302,329]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/ON156523" pageId="8" pageNumber="193">ON156523</accessionNumber>
; 
<collectionCode id="ED061CACFF9D1678FC4D4573FBD9B302" box="[1000,1078,303,329]" country="Thailand" httpUri="http://grbio.org/cool/672j-mk16" name="Mae Fah Laung University Herbarium" pageId="8" pageNumber="193">MFLU</collectionCode>
22-0056: 
<collectionCode id="ED061CACFF9D1678FB024572FB3EB303" box="[1191,1233,302,328]" pageId="8" pageNumber="193">ITS</collectionCode>
– 
<accessionNumber id="9444198AFF9D1678FB454572FAB1B303" box="[1248,1374,302,328]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/ON176689" pageId="8" pageNumber="193">ON176689</accessionNumber>
, 
<collectionCode id="ED061CACFF9D1678FACD4572FA72B303" box="[1384,1437,302,328]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15590" lsid="urn:lsid:biocol.org:col:15590" name="Louisiana State University - Herbarium" pageId="8" pageNumber="193" type="Herbarium">LSU</collectionCode>
– 
<accessionNumber id="9444198AFF9D1678FF2D450EFEEAB326" box="[136,261,338,365]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/ON325438" pageId="8" pageNumber="193">ON325438</accessionNumber>
</paragraph>
<paragraph id="8BA88469FF9D1678FF18452BFDE7B09F" blockId="8.[136,1452,158,725]" pageId="8" pageNumber="193">
Notes: In the current study, 
<taxonomicName id="4C17FFEAFF9D1678FE5B452AFD33B3DB" box="[510,732,374,400]" class="Leotiomycetes" family="Pezizellaceae" genus="Bisporella" kingdom="Fungi" order="Rhytismatales" pageId="8" pageNumber="193" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF9D1678FE5B452AFD33B3DB" box="[510,732,374,400]" italics="true" pageId="8" pageNumber="193">Bisporella montana</emphasis>
</taxonomicName>
(
<collectionCode id="ED061CACFF9D1678FD48452AFCAFB3DB" box="[749,832,374,400]" country="China" httpUri="http://grbio.org/cool/qht3-g0qs" name="Chinese Academy of Sciences" pageId="8" pageNumber="193" type="Herbarium">HMAS</collectionCode>
275566, 
<typeStatus id="54AC3ACBFF9D1678FC09452AFBFFB3DB" box="[940,1040,374,400]" pageId="8" pageNumber="193" type="holotype">holotype</typeStatus>
) and our specimens clustered under 
<taxonomicName id="4C17FFEAFF9D1678FF2D45C6FF02B3FF" baseAuthorityName="Baral" baseAuthorityYear="1989" box="[136,237,410,436]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="8" pageNumber="193" phylum="Ascomycota" rank="genus">
<emphasis id="B963587BFF9D1678FF2D45C6FF02B3FF" box="[136,237,410,436]" italics="true" pageId="8" pageNumber="193">Calycina</emphasis>
</taxonomicName>
, as 
<taxonomicName id="4C17FFEAFF9D1678FEB945C6FE04B3FF" authorityName="Lestari &amp; Thilini Chethana" authorityYear="2022" baseAuthorityName="W. Y. Zhuang &amp; H. D. Zheng" baseAuthorityYear="2022" box="[284,491,410,436]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="8" pageNumber="193" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF9D1678FEB945C6FE04B3FF" box="[284,491,410,436]" italics="true" pageId="8" pageNumber="193">Calycina montana</emphasis>
</taxonomicName>
. 
<taxonomicName id="4C17FFEAFF9D1678FE5F45C6FD25B3FF" authorityName="Lestari &amp; Thilini Chethana" authorityYear="2022" baseAuthorityName="W. Y. Zhuang &amp; H. D. Zheng" baseAuthorityYear="2022" box="[506,714,410,436]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="8" pageNumber="193" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF9D1678FE5F45C6FD25B3FF" box="[506,714,410,436]" italics="true" pageId="8" pageNumber="193">Calycina montana</emphasis>
</taxonomicName>
(
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275566), previously known as 
<taxonomicName id="4C17FFEAFF9D1678FB3645C6FA9EB3FF" box="[1171,1393,410,436]" class="Leotiomycetes" family="Pezizellaceae" genus="Bisporella" kingdom="Fungi" order="Rhytismatales" pageId="8" pageNumber="193" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF9D1678FB3645C6FA9EB3FF" box="[1171,1393,410,436]" italics="true" pageId="8" pageNumber="193">Bisporella montana</emphasis>
</taxonomicName>
, was discovered in a mountainous area in 
<collectingCountry id="F300C4F9FF9D1678FD8245E2FD85B393" box="[551,618,446,472]" name="China" pageId="8" pageNumber="193">China</collectingCountry>
as a saprobe on woody substrates (
<bibRefCitation id="EF86F998FF9D1678FC5C45E2FB3AB393" author="Zhuang, W. Y. &amp; Zheng, H. D. &amp; Ren, F." box="[1017,1237,446,473]" pageId="8" pageNumber="193" pagination="401 - 420" refId="ref12820" refString="Zhuang, W. Y., Zheng, H. D. &amp; Ren, F. (2017) Taxonomy of the genus Bisporella (Helotiales) in China with seven new species and four new records. Mycosystema 36: 401 - 420. https: // doi. org / 10.13346 / j. mycosystema. 160193" type="journal article" year="2017">
Zhuang 
<emphasis id="B963587BFF9D1678FBFD45E3FB65B393" box="[1112,1162,446,472]" italics="true" pageId="8" pageNumber="193">et al</emphasis>
. 2017
</bibRefCitation>
). The characters of both specimens, especially the amber-colored apothecia and gelatinized 
<emphasis id="B963587BFF9D1678FC6945BFFB6AB3B7" box="[972,1157,483,508]" italics="true" pageId="8" pageNumber="193">textura porrecta</emphasis>
cells of ectal excipulum, fit the generic concept of 
<taxonomicName id="4C17FFEAFF9D1678FE10465AFD58B06B" authority="(Baral 1993)" baseAuthorityName="Baral" baseAuthorityYear="1993" box="[437,695,518,544]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="8" pageNumber="193" phylum="Ascomycota" rank="genus">
<emphasis id="B963587BFF9D1678FE10465AFDF5B06B" box="[437,538,518,544]" italics="true" pageId="8" pageNumber="193">Calycina</emphasis>
(
<bibRefCitation id="EF86F998FF9D1678FD8E465AFD40B06B" author="Baral, H. O." box="[555,687,518,544]" pageId="8" pageNumber="193" pagination="3 - 22" refId="ref7624" refString="Baral, H. O. (1993) Beitrage zur taxonomie der discomyceten III. Zeitschrift Fur Mykologie 59: 3 - 22. [in Germany]" type="journal article" year="1993">Baral 1993</bibRefCitation>
)
</taxonomicName>
. Another character which further confirms the combination is the appearance of a calycina-like apical ring shown clearly in our collection (
<figureCitation id="132C98ECFF9D1678FC714676FBD1B00F" box="[980,1086,554,580]" captionStart="FIGURE 3" captionStartId="6.[136,229,1910,1932]" captionTargetBox="[205,1380,202,1874]" captionTargetId="figure-24@6.[183,1404,190,1885]" captionTargetPageId="6" captionText="FIGURE 3. Calycina montana (MFLU 22-0055, new geographical record). a Close up of excipulum cells. b Ectal excipulum cells (b mounted in Congo Red). c Medullary excipulum cells. d–e Filiform paraphyses with refractive guttules. f–g Asci (mounted in Congo red). h Asci (alive state). i Close up of crozier. j Tip of the ascus (J+, in Melzerr’s agent with KOH treatment). k–m Ascospores (m mounted in Congo red). Scale bars: a = 40 μm, b–c = 25 μm, d–h = 14 μm, i = 10 μm, j–m = 4 μm." figureDoi="http://doi.org/10.5281/zenodo.7002949" httpUri="https://zenodo.org/record/7002949/files/figure.png" pageId="8" pageNumber="193">Figure 3j</figureCitation>
) and the type specimen (
<figureCitation id="132C98ECFF9D1678FAC54676FF5FB023" captionStart="FIGURE 4" captionStartId="7.[136,229,1902,1924]" captionTargetBox="[204,1383,192,1871]" captionTargetId="figure-17@7.[183,1403,190,1878]" captionTargetPageId="7" captionText="FIGURE 4. Calycina montana (HMAS 275566, holotype). a A herbarium package. b An apothecium on the woody substrate. c–d Cross sections of an apothecium (d mounted in Congo red). e Close up of hymenium at the margin. f Close up of ectal excipulum cells. g–h Filiform paraphyses (dead state). i–l Asci (k mounted in Melzer’s agent, l mounted in Congo red). m Tip of the ascus (J+, in Melzer’s agent with KOH treatment). n Close up of crozier at the base of an immature ascus. o–p Ascospores (o mounted in Congo red). Scale bars: b = 500 μm, c–d = 380 μm, e = 43 μm, f = 25 μm, g–h = 23 μm, i–l = 19 μm, m = 4 μm, n = 8 μm, m, o–p = 4 μm." figureDoi="http://doi.org/10.5281/zenodo.7002953" httpUri="https://zenodo.org/record/7002953/files/figure.png" pageId="8" pageNumber="193">
Figure 
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</figureCitation>
). Based on phylogenetic analyses using 
<collectionCode id="ED061CACFF9D1678FDD84612FD48B023" box="[637,679,590,616]" pageId="8" pageNumber="193">ITS</collectionCode>
and 
<collectionCode id="ED061CACFF9D1678FD444612FCF9B023" box="[737,790,590,616]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15590" lsid="urn:lsid:biocol.org:col:15590" name="Louisiana State University - Herbarium" pageId="8" pageNumber="193" type="Herbarium">LSU</collectionCode>
sequence data, our collection clusters with the type strain with high statistical support of 99% 
<collectionCode id="ED061CACFF9D1678FD85462FFDA6B0C6" box="[544,585,627,653]" country="France" name="Musee de Lectoure" pageId="8" pageNumber="193">ML</collectionCode>
and 0.99 BI (
<figureCitation id="132C98ECFF9D1678FD47462EFCADB0C7" box="[738,834,626,652]" captionStart="FIGURE 1" captionStartId="4.[136,229,1993,2015]" captionTargetBox="[311,1278,617,1968]" captionTargetId="figure-211@4.[293,1295,605,1968]" captionTargetPageId="4" captionText="FIGURE 1. The phylogram of combined ITS and LSU sequence data for genera in Pezizellaceae. Maximum likelihood bootstrap values greater than 70% and posterior probability values greater than 0.95 given near the nodes. The new geographical record highlighted in red and type strains are in bold. The tree is rooted to Chlorencoelia torta (JAC 14068, KUSF 52256 and ICMP 21732)." figureDoi="http://doi.org/10.5281/zenodo.7002945" httpUri="https://zenodo.org/record/7002945/files/figure.png" pageId="8" pageNumber="193">Figure 1</figureCitation>
). Across the 466 base pairs of 
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(+ 
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) gene of our 
<taxonomicName id="4C17FFEAFF9D1678FF2D46CAFEB8B0FB" authorityName="Lestari &amp; Thilini Chethana" authorityYear="2022" baseAuthorityName="W. Y. Zhuang &amp; H. D. Zheng" baseAuthorityYear="2022" box="[136,343,662,688]" class="Leotiomycetes" family="Pezizellaceae" genus="Calycina" kingdom="Fungi" order="Rhytismatales" pageId="8" pageNumber="193" phylum="Ascomycota" rank="species" species="montana">
<emphasis id="B963587BFF9D1678FF2D46CAFEB8B0FB" box="[136,343,662,688]" italics="true" pageId="8" pageNumber="193">Calycina montana</emphasis>
</taxonomicName>
strains (
<collectionCode id="ED061CACFF9D1678FE1D46CAFDE7B0FB" box="[440,520,662,688]" country="Thailand" httpUri="http://grbio.org/cool/672j-mk16" name="Mae Fah Laung University Herbarium" pageId="8" pageNumber="193">MFLU</collectionCode>
22-0055 and 
<collectionCode id="ED061CACFF9D1678FD0046CBFD1CB0FA" box="[677,755,663,689]" country="Thailand" httpUri="http://grbio.org/cool/672j-mk16" name="Mae Fah Laung University Herbarium" pageId="8" pageNumber="193">MFLU</collectionCode>
22-0056) and the ex-type 
<collectionCode id="ED061CACFF9D1678FB8546CAFB9EB0FB" box="[1056,1137,662,688]" country="China" httpUri="http://grbio.org/cool/qht3-g0qs" name="Chinese Academy of Sciences" pageId="8" pageNumber="193" type="Herbarium">HMAS</collectionCode>
275566, only one base pair difference (0.22 %) was observed.
</paragraph>
</subSubSection>
</treatment>
</document>