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<document id="308D0153DCAE8DD210F5C7CD76B67759" ID-CLB-Dataset="298878" ID-DOI="10.4467/16890027AP.15.004.2191" ID-GBIF-Dataset="7659f67c-19dc-4006-8569-71eecf062002" ID-ISSN="1689-0027" ID-Zenodo-Dep="10994283" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="karina" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="karina" IM.treatments_approvedBy="karina" checkinTime="1713466879163" checkinUser="felipe" docAuthor="Wylezich, Claudia, Walochnik, Julia, Corsaro, Daniele, Michel, Rolf &amp; Kudryavtsev, Alexander" docDate="2015" docId="03B787916341FFFEFF3AF9B4A89BFC73" docLanguage="en" docName="ActaProtozool.54.1.45-51.pdf" docOrigin="Acta Protozoologica 54 (1)" docSource="https://www.mendeley.com/catalogue/757ccadd-6278-3e79-a24d-67b5e2845435/" docStyle="DocumentStyle:64ABA4BEA387EE5C24A0DDEB32CC92E7.3:ActaProtozool.2014-.journal_article" docStyleId="64ABA4BEA387EE5C24A0DDEB32CC92E7" docStyleName="ActaProtozool.2014-.journal_article" docStyleVersion="3" docTitle="Sappinia platani Wylezich &amp; Walochnik &amp; Corsaro &amp; Michel &amp; Kudryavtsev 2015, sp. nov." docType="treatment" docVersion="5" lastPageNumber="50" masterDocId="FF8EFFE96344FFFBFF9EFFA7AD35FFE0" masterDocTitle="Electron Microscopical Investigations of a New Species of the Genus Sappinia (Thecamoebidae, Amoebozoa), Sappinia platani sp. nov., Reveal a Dictyosome in this Genus" masterLastPageNumber="51" masterPageNumber="45" pageNumber="50" updateTime="1719450946863" updateUser="karina" zenodo-license-document="CC-BY-4.0">
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<mods:title id="41DAFDEA9681B6485F14CE790F0D0F26">Electron Microscopical Investigations of a New Species of the Genus Sappinia (Thecamoebidae, Amoebozoa), Sappinia platani sp. nov., Reveal a Dictyosome in this Genus</mods:title>
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<mods:namePart id="8B7BCC98D7E462D3F30E96CF0EA14BD0">Wylezich, Claudia</mods:namePart>
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<mods:namePart id="50178DD0A3AB08BCFBB2BF9B6397B838">Walochnik, Julia</mods:namePart>
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<mods:namePart id="C44E5BED5965DAB4599BA6B6F46C0741">Michel, Rolf</mods:namePart>
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<treatment id="03B787916341FFFEFF3AF9B4A89BFC73" ID-DOI="http://doi.org/10.5281/zenodo.12521610" ID-GBIF-Taxon="233761193" ID-Zenodo-Dep="12521610" LSID="urn:lsid:plazi:treatment:03B787916341FFFEFF3AF9B4A89BFC73" httpUri="http://treatment.plazi.org/id/03B787916341FFFEFF3AF9B4A89BFC73" lastPageNumber="50" pageId="5" pageNumber="50">
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<paragraph id="8BA136876341FFFEFF3AF9B4AF6CF9B0" blockId="5.[127,771,1555,1616]" pageId="5" pageNumber="50">
<heading id="D0E981EB6341FFFEFF3AF9B4AF6CF9B0" fontSize="11" level="2" pageId="5" pageNumber="50" reason="5">
<emphasis id="B96AEA956341FFFEFF3AF9B4AF6CF9B0" bold="true" pageId="5" pageNumber="50">
<taxonomicName id="4C1E4D046341FFFEFF3AF9B4AC47F9CE" authority="Wylezich &amp; Walochnik &amp; Corsaro &amp; Michel &amp; Kudryavtsev, 2015" authorityName="Wylezich &amp; Walochnik &amp; Corsaro &amp; Michel &amp; Kudryavtsev" authorityYear="2015" box="[164,370,1555,1582]" class="Discosea" family="Stenamoebidae" genus="Sappinia" higherTaxonomySource="GBIF" kingdom="Protozoa" order="Thecamoebida" pageId="5" pageNumber="50" phylum="Amoebozoa" rank="species" species="platani" status="sp. nov.">
<emphasis id="B96AEA956341FFFEFF3AF9B4AC47F9CE" bold="true" box="[164,370,1555,1582]" italics="true" pageId="5" pageNumber="50">Sappinia platani</emphasis>
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<taxonomicNameLabel id="A25957EE6341FFFEFEE0F9B4ACD7F9CD" box="[382,482,1555,1581]" pageId="5" pageNumber="50" rank="species">sp. nov.</taxonomicNameLabel>
Wylezich, Walochnik, Corsaro, Michel et Kudryavtsev, 2015
</emphasis>
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<subSubSection id="C304650C6341FFFEFF3AF9C0AC19F878" pageId="5" pageNumber="50" type="description">
<paragraph id="8BA136876341FFFEFF3AF9C0AC19F878" blockId="5.[127,773,1639,1944]" pageId="5" pageNumber="50">
<emphasis id="B96AEA956341FFFEFF3AF9C0AC0AF961" bold="true" box="[164,319,1639,1665]" pageId="5" pageNumber="50">Description:</emphasis>
Locomotive forms oblong, of a lingulate morphotype; often irregular, length 5776 µm (n = 10), breadth 2338 µm (n = 10). Two nuclei in each trophic cell; nuclei vesicular, with large, central nucleoli. Cysts formed by two individual cells, 2330 µm in diameter. Cell coat
<quantity id="4CE69B626341FFFEFE28F8B2AF0EF8D0" box="[438,571,1813,1840]" metricMagnitude="-8" metricUnit="m" metricValue="9.0" metricValueMax="12.0" metricValueMin="6.0" pageId="5" pageNumber="50" unit="nm" value="90.0" valueMax="120.0" valueMin="60.0">60120 nm</quantity>
thick, consisting of a loose layer of bent, filamentous structures. Standing forms as typical for
<taxonomicName id="4C1E4D046341FFFEFE3CF8FCAF24F895" authorityName="Dangeard" authorityYear="1896" box="[418,529,1883,1909]" class="Discosea" family="Stenamoebidae" genus="Sappinia" higherTaxonomySource="GBIF" kingdom="Protozoa" pageId="5" pageNumber="50" phylum="Amoebozoa" rank="species" species="pedata">
<emphasis id="B96AEA956341FFFEFE3CF8FCAF24F895" box="[418,529,1883,1909]" italics="true" pageId="5" pageNumber="50">S. pedata</emphasis>
</taxonomicName>
were extremely seldom observed.
</paragraph>
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<subSubSection id="C304650C6341FFFEFCD1FF78A918FE2B" pageId="5" pageNumber="50" type="materials_examined">
<paragraph id="8BA136876341FFFEFCD1FF78AEA9FE65" blockId="5.[808,1455,223,915]" pageId="5" pageNumber="50">
<heading id="D0E981EB6341FFFEFCD1FF78AEA9FE65" fontSize="11" level="2" pageId="5" pageNumber="50" reason="5">
<emphasis id="B96AEA956341FFFEFCD1FF78A933FF19" bold="true" box="[847,1030,223,249]" pageId="5" pageNumber="50">Type material:</emphasis>
The type strain PL-247 is deposited with CCAP under the accession number 1575/4.
<emphasis id="B96AEA956341FFFEFCD1FE82A935FEDF" bold="true" box="[847,1024,293,319]" pageId="5" pageNumber="50">Type locality:</emphasis>
Specimen of the type strain was collected at the bark of sycamore tree in Koblenz,
<collectingCountry id="F30976176341FFFEFCB7FECCAEADFE65" box="[809,920,363,389]" name="Germany" pageId="5" pageNumber="50">Germany</collectingCountry>
.
</heading>
</paragraph>
<paragraph id="8BA136876341FFFEFCD1FE29A918FE2B" blockId="5.[808,1455,223,915]" pageId="5" pageNumber="50">
<heading id="D0E981EB6341FFFEFCD1FE29A918FE2B" fontSize="11" level="2" pageId="5" pageNumber="50" reason="5">
<emphasis id="B96AEA956341FFFEFCD1FE29A92FFE48" bold="true" box="[847,1050,398,424]" pageId="5" pageNumber="50">Type sequence:</emphasis>
EU881943 (SSU rRNA gene,
<bibRefCitation id="EF8F4B766341FFFEFCB7FE16A914FE2B" author="Wylezich C. &amp; Walochnik J. &amp; Michel R." box="[809,1057,433,460]" pageId="5" pageNumber="50" pagination="869 - 873" refId="ref4712" refString="Wylezich C., Walochnik J., Michel R. (2009) High genetic diversity of Sappinia - like strains (Amoebozoa, Thecamoebidae) revealed by SSU rRNA investigations. Parasitol. Res. 105: 869 - 873" type="journal article" year="2009">
Wylezich
<emphasis id="B96AEA956341FFFEFC01FE15AEE7FE2B" box="[927,978,433,459]" italics="true" pageId="5" pageNumber="50">et al</emphasis>
. 2009
</bibRefCitation>
).
</heading>
</paragraph>
</subSubSection>
<subSubSection id="C304650C6341FFFEFCD1FE73AEB8FDB7" pageId="5" pageNumber="50" type="etymology">
<paragraph id="8BA136876341FFFEFCD1FE73AEB8FDB7" blockId="5.[808,1455,223,915]" pageId="5" pageNumber="50">
<emphasis id="B96AEA956341FFFEFCD1FE73AEEAFE0E" bold="true" box="[847,991,468,494]" pageId="5" pageNumber="50">Etymology:</emphasis>
The specific epithet is derived from the sycamore tree
<taxonomicName id="4C1E4D046341FFFEFB98FE50A833FDF1" box="[1030,1286,503,529]" class="Magnoliopsida" family="Platanaceae" genus="Platanus" kingdom="Plantae" order="Proteales" pageId="5" pageNumber="50" phylum="Tracheophyta" rank="species" species="occidentalis">
<emphasis id="B96AEA956341FFFEFB98FE50A833FDF1" box="[1030,1286,503,529]" italics="true" pageId="5" pageNumber="50">Platanus occidentalis</emphasis>
</taxonomicName>
(Plantae, Angiospermae,
<taxonomicName id="4C1E4D046341FFFEFC5EFDBDA91BFDD5" box="[960,1070,538,565]" class="Magnoliopsida" kingdom="Plantae" order="Proteales" pageId="5" pageNumber="50" phylum="Tracheophyta" rank="order">Proteales</taxonomicName>
) from which the
<typeStatus id="54A588256341FFFEFB65FDBCA81BFDD5" box="[1275,1326,539,565]" pageId="5" pageNumber="50">type</typeStatus>
strain was isolated.
</paragraph>
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<subSubSection id="C304650C6341FFFEFCD1FDC7AED3FCCA" pageId="5" pageNumber="50" type="diagnosis">
<paragraph id="8BA136876341FFFEFCD1FDC7AED3FCCA" blockId="5.[808,1455,223,915]" pageId="5" pageNumber="50">
<emphasis id="B96AEA956341FFFEFCD1FDC7A950FD9A" bold="true" box="[847,1125,608,634]" pageId="5" pageNumber="50">Differential diagnosis:</emphasis>
The species presented here differs from
<taxonomicName id="4C1E4D046341FFFEFC5AFD24A961FD7D" baseAuthorityName="Hartmann and Nagler" baseAuthorityYear="1908" box="[964,1108,643,669]" class="Discosea" family="Stenamoebidae" genus="Sappinia" higherTaxonomySource="GBIF" kingdom="Protozoa" pageId="5" pageNumber="50" phylum="Amoebozoa" rank="species" species="diploidea">
<emphasis id="B96AEA956341FFFEFC5AFD24A961FD7D" box="[964,1108,643,669]" italics="true" pageId="5" pageNumber="50">S. diploidea</emphasis>
</taxonomicName>
in having slightly larger locomotive forms and smaller cysts, presence of a dictyosome, and a different
<typeStatus id="54A588256341FFFEFBCDFD6DA9B3FD04" box="[1107,1158,714,740]" pageId="5" pageNumber="50">type</typeStatus>
of a cell coat.
<taxonomicName id="4C1E4D046341FFFEFAA3FD6EA89AFD03" authorityName="Wylezich &amp; Walochnik &amp; Corsaro &amp; Michel &amp; Kudryavtsev" authorityYear="2015" box="[1341,1455,713,739]" class="Discosea" family="Stenamoebidae" genus="Sappinia" higherTaxonomySource="GBIF" kingdom="Protozoa" pageId="5" pageNumber="50" phylum="Amoebozoa" rank="species" species="platani">
<emphasis id="B96AEA956341FFFEFAA3FD6EA89AFD03" box="[1341,1455,713,739]" italics="true" pageId="5" pageNumber="50">S. platani</emphasis>
</taxonomicName>
differs from both known species based in SSU rRNA gene sequences.
</paragraph>
</subSubSection>
<subSubSection id="C304650C6341FFFEFCD1FC95A89BFC73" pageId="5" pageNumber="50" type="discussion">
<paragraph id="8BA136876341FFFEFCD1FC95A89BFC73" blockId="5.[808,1455,223,915]" pageId="5" pageNumber="50">
<emphasis id="B96AEA956341FFFEFCD1FC95AEFFFCAC" bold="true" box="[847,970,818,844]" pageId="5" pageNumber="50">Remarks:</emphasis>
The granuloplasm of the strain investigated here contains dictyosomes and endocytobionts of the genus
<taxonomicName id="4C1E4D046341FFFEFC07FCDFA915FC72" box="[921,1056,888,914]" class="Sphingobacteriia" family="Sphingobacteriaceae" genus="Pedobacter" kingdom="Bacteria" order="Sphingobacteriales" pageId="5" pageNumber="50" phylum="Bacteroidetes" rank="genus">
<emphasis id="B96AEA956341FFFEFC07FCDFA915FC72" box="[921,1056,888,914]" italics="true" pageId="5" pageNumber="50">Pedobacter</emphasis>
</taxonomicName>
(Bacteroidetes, Sphingobacteria).
</paragraph>
</subSubSection>
</treatment>
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