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<document id="7CB3CD5E1E9C74D4A6ADA7C759BEF291" ID-DOI="10.11646/phytotaxa.317.3.2" ID-ISSN="1179-3163" ID-Zenodo-Dep="13698495" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1725570356103" checkinUser="felipe" docAuthor="Solís, Karina, Barriuso, Juan José, Garcés-Claver, Ana &amp; González, Vicente" docDate="2017" docId="038087D65125FFBAA3F6172DFCDBF828" docLanguage="en" docName="phytotaxa.317.3.2.pdf" docOrigin="Phytotaxa 317 (3)" docSource="http://dx.doi.org/10.11646/phytotaxa.317.3.2" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Tulasnella tubericola K. Solis, J. Barriuso, A. Garces-Claver &amp; V. Gonzalez 2017, sp. nov." docType="treatment" docVersion="1" lastPageNumber="181" masterDocId="FFB9FFAE5123FFBCA37E143BFF80FFFB" masterDocTitle="Tulasnella tubericola (Tulasnellaceae, Cantharellales, Basidiomycota): a new Rhizoctonia-like fungus associated with mycorrhizal evergreen oak plants artificially inoculated with black truffle (Tuber melanosporum) in Spain" masterLastPageNumber="187" masterPageNumber="175" pageNumber="181" updateTime="1725577492520" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="404CA3B1C82695DC85BC11781367BA60">Tulasnella tubericola (Tulasnellaceae, Cantharellales, Basidiomycota): a new Rhizoctonia-like fungus associated with mycorrhizal evergreen oak plants artificially inoculated with black truffle (Tuber melanosporum) in Spain</mods:title>
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<mods:namePart id="4B3D5953BEF40D0970F3DA2F1044A49C">Solís, Karina</mods:namePart>
<mods:affiliation id="93F7B259D49906C4F53709791591E6E8">Universidad de Zaragoza / IA 2 (Instituto Agroalimentario de Aragón) &amp; Instituto Nacional de Investigaciones Agropecuarias (INIAP) Quito, Ecuador</mods:affiliation>
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<mods:namePart id="9AD73768BB1AE514E8CE5E82FBDF60FF">Barriuso, Juan José</mods:namePart>
<mods:affiliation id="3B9EF4F2C9DF922F59BB3AA661349205">Universidad de Zaragoza / IA 2 (Instituto Agroalimentario de Aragón)</mods:affiliation>
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<mods:title id="089A073F3CD591C47E0F493394280CC6">Phytotaxa</mods:title>
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<paragraph id="8B9636C05125FFBAA3F6172DFB21FCD4" blockId="6.[136,1185,790,815]" box="[136,1185,790,815]" pageId="6" pageNumber="181">
<heading id="D0DE81AC5125FFBAA3F6172DFB21FCD4" box="[136,1185,790,815]" fontSize="11" level="2" pageId="6" pageNumber="181" reason="3">
<taxonomicName id="4C294D435125FFBAA3F6172DFC65FCD4" authority="K. Solis, J. Barriuso, A. Garces-Claver &amp; V. Gonzalez" authorityName="K. Solis, J. Barriuso, A. Garces-Claver &amp; V. Gonzalez" authorityYear="2017" box="[136,997,790,815]" class="Agaricomycetes" family="Tulasnellaceae" genus="Tulasnella" kingdom="Fungi" order="Cantharellales" pageId="6" pageNumber="181" phylum="Basidiomycota" rank="species" species="tubericola" status="sp. nov.">
<emphasis id="B95DEAD25125FFBAA3F6172DFEFEFCD4" bold="true" box="[136,382,790,815]" italics="true" pageId="6" pageNumber="181">Tulasnella tubericola</emphasis>
K. Solís, J. Barriuso, A. Garcés-Claver &amp; V. González
</taxonomicName>
,
<taxonomicNameLabel id="A26E57A95125FFBAA08E172DFBC6FCD4" box="[1008,1094,790,815]" pageId="6" pageNumber="181" rank="species">
<emphasis id="B95DEAD25125FFBAA08E172DFBBFFCD4" box="[1008,1087,790,815]" italics="true" pageId="6" pageNumber="181">sp. nov</emphasis>
.
</taxonomicNameLabel>
(
<figureCitation id="13122A455125FFBAA728172DFB16FCD4" box="[1110,1174,790,815]" captionStart="FIGURE 1" captionStartId="7.[136,229,1426,1447]" captionTargetBox="[161,1436,171,1397]" captionTargetId="figure-14@7.[151,1436,171,1397]" captionTargetPageId="7" captionText="FIGURE 1. Tulasnella tubericola CECT 20958 (holotype). a-c. morphological aspect in PDA culture (front view) at several temperatures; d. runner hyphae; e. monilioid-like hyphal elements; f. chains of monilioid cells; g. bi- and tri-nucleate hyphae" figureDoi="http://doi.org/10.5281/zenodo.13698497" httpUri="https://zenodo.org/record/13698497/files/figure.png" pageId="6" pageNumber="181">Fig. 1</figureCitation>
)
</heading>
</paragraph>
<paragraph id="8B9636C05125FFBAA3F61707FEE6FCAA" blockId="6.[136,1452,828,993]" box="[136,358,828,849]" pageId="6" pageNumber="181">MycoBank MB812221</paragraph>
</subSubSection>
<subSubSection id="C333654B5125FFBAA3F6175BFAB7F900" pageId="6" pageNumber="181" type="materials_examined">
<paragraph id="8B9636C05125FFBAA3F6175BFADCFC1A" blockId="6.[136,1452,828,993]" pageId="6" pageNumber="181">
Type:—
<collectingCountry id="F33E76505125FFBAA3A8175BFE9DFC8E" box="[214,285,864,885]" name="Spain" pageId="6" pageNumber="181">SPAIN</collectingCountry>
:
<collectingRegion id="49EDF8225125FFBAA259175BFE12FC8E" box="[295,402,864,885]" country="Spain" name="Aragon" pageId="6" pageNumber="181">ARAGÓN</collectingRegion>
: Zaragoza, Centro de Investigación y Tecnología Agroalimentaria de Aragón (
<collectionCode id="ED38AE055125FFBAA7EF175BFB4CFC8E" box="[1169,1228,864,885]" country="0" httpUri="http://biocol.org/urn:lsid:biocol.org:col:14197" lsid="urn:lsid:biocol.org:col:14197" name="The Citadel" pageId="6" pageNumber="181" type="Herbarium">CITA</collectionCode>
),
<geoCoordinate id="EE1D50075125FFBAA7A0175BFA09FC8E" box="[1246,1417,864,885]" degrees="41" direction="north" minutes="43" orientation="latitude" pageId="6" pageNumber="181" precision="1" seconds="28.48" value="41.72458">41° 43 28.48“ N</geoCoordinate>
,
<geoCoordinate id="EE1D50075125FFBAA6E8175BFEBFFC62" degrees="0" direction="west" minutes="48" orientation="longitude" pageId="6" pageNumber="181" precision="1" seconds="31.09" value="-0.8086361">0° 48 31.09“ W</geoCoordinate>
, 230 msl, in greenhouse, in culture pots, colonies in
<collectionCode id="ED38AE055125FFBAA04717BFFCE8FC62" box="[825,872,900,921]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="6" pageNumber="181" type="Herbarium">PDA</collectionCode>
isolated from ectomycorrhizal tips of one-year
<taxonomicName id="4C294D435125FFBAA64C17BFFEC4FC46" class="Magnoliopsida" family="Fagaceae" genus="Quercus" kingdom="Plantae" order="Fagales" pageId="6" pageNumber="181" phylum="Tracheophyta" rank="subSpecies" species="ilex" subSpecies="ballota">
<emphasis id="B95DEAD25125FFBAA64C17BFFA2CFC62" box="[1330,1452,900,921]" italics="true" pageId="6" pageNumber="181">Quercus ilex</emphasis>
subsp.
<emphasis id="B95DEAD25125FFBAA27E1793FEC4FC46" box="[256,324,936,957]" italics="true" pageId="6" pageNumber="181">ballota</emphasis>
</taxonomicName>
plants artificially mycorrhized with
<taxonomicName id="4C294D435125FFBAA1DD1793FCF0FC46" box="[675,880,936,957]" class="Agaricomycetes" family="Paxillaceae" genus="Tuber" kingdom="Fungi" order="Boletales" pageId="6" pageNumber="181" phylum="Basidiomycota" rank="species" species="melanosporum">
<emphasis id="B95DEAD25125FFBAA1DD1793FCF0FC46" box="[675,880,936,957]" italics="true" pageId="6" pageNumber="181">Tuber melanosporum</emphasis>
</taxonomicName>
,
<date id="FF9710005125FFBAA0051793FC76FC46" box="[891,1014,936,957]" pageId="6" pageNumber="181" value="2015-04-09">9 April 2015</date>
,
<emphasis id="B95DEAD25125FFBAA77E1793FBD1FC46" box="[1024,1105,936,957]" italics="true" pageId="6" pageNumber="181">
<collectionCode id="ED38AE055125FFBAA77E1793FB93FC46" box="[1024,1043,936,957]" country="United Kingdom" lsid="urn:lsid:biocol.org:col:15867" name="Royal Botanic Gardens" pageId="6" pageNumber="181" type="Herbarium">K</collectionCode>
. Solís,
</emphasis>
<typeStatus id="549288625125FFBAA7291793FB2BFC46" box="[1111,1195,936,957]" pageId="6" pageNumber="181" type="holotype">holotype</typeStatus>
strain EP-15 (deposited as living culture in the Spanish Type Culture Collection under the number
<collectionCode id="ED38AE055125FFBAA01117F7FC2CFC1A" box="[879,940,972,993]" country="Spain" httpUri="http://grbio.org/cool/n6ez-ekac" name="Coleccion Espanola de Cultivos Tipo" pageId="6" pageNumber="181">CECT</collectionCode>
20958), ITS sequence GenBank KX929166.
</paragraph>
<paragraph id="8B9636C05125FFBAA3F61029FD05FB40" blockId="6.[136,1452,1042,2003]" pageId="6" pageNumber="181">
Diagnosis:—This species is characterized by the relatively fast growing-rate of its colonies in
<collectionCode id="ED38AE055125FFBAA7A41029FA90FBD0" box="[1242,1296,1042,1067]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="6" pageNumber="181" type="Herbarium">PDA</collectionCode>
medium that exhibits whitish-cream to pale orange-ochraceous tones, its radially filamentous appearance when young that become glossy in older cultures, thin-walled runner hyphae, the production of both typically barrel-shaped to globose monilioid cells together with terminal, finger-like, clavate or even subcapitate monilioid hyphal ends produced in restricted areas of aged cultures and its unique ITS sequence.
</paragraph>
<paragraph id="8B9636C05125FFBAA3C310FDFAB7F900" blockId="6.[136,1452,1042,2003]" pageId="6" pageNumber="181">
<emphasis id="B95DEAD25125FFBAA3C310FDFEA1FB24" box="[189,289,1222,1247]" italics="true" pageId="6" pageNumber="181">Colonies</emphasis>
in
<collectionCode id="ED38AE055125FFBAA23D10FDFEF9FB24" box="[323,377,1222,1247]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="6" pageNumber="181" type="Herbarium">PDA</collectionCode>
and
<collectionCode id="ED38AE055125FFBAA2D110FDFE6DFB24" box="[431,493,1222,1247]" country="United Kingdom" httpUri="http://biocol.org/urn:lsid:biocol.org:col:12899" lsid="urn:lsid:biocol.org:col:12899" name="Crayford Manor House Adult Education Centre" pageId="6" pageNumber="181" type="Herbarium">CMA</collectionCode>
circular, filamentous, slow to moderately fast growing; in
<collectionCode id="ED38AE055125FFBAA70710FDFB2FFB24" box="[1145,1199,1222,1247]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="6" pageNumber="181" type="Herbarium">PDA</collectionCode>
colour at first white to cream-white, becoming pale cream to straw-ochraceous (2.5
<collectionCode id="ED38AE055125FFBAA05810D1FCBBFAF8" box="[806,827,1258,1283]" country="USA" lsid="urn:lsid:biocol.org:col:14792" name="Yale University" pageId="6" pageNumber="181" type="Herbarium">Y</collectionCode>
/8/3) in older cultures, paler towards the centre (5
<collectionCode id="ED38AE055125FFBAA62410D1FAF1FAF8" box="[1370,1393,1258,1283]" country="USA" lsid="urn:lsid:biocol.org:col:14792" name="Yale University" pageId="6" pageNumber="181" type="Herbarium">Y</collectionCode>
/8/3), flat to slightly raised profile with scarce aerial or tufted hyphae; radially filamentous appearance with adpressed hyphae in young colonies, granular to furfuraceous in older colonies, exhibiting a mucoid, shinny or glossy nature in some
<collectionCode id="ED38AE055125FFBAA3B4116DFE80FA94" box="[202,256,1366,1391]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="6" pageNumber="181" type="Herbarium">PDA</collectionCode>
cultures; true
<emphasis id="B95DEAD25125FFBAA2DD116DFD84FA94" box="[419,516,1366,1391]" italics="true" pageId="6" pageNumber="181">sclerotia</emphasis>
absent but slightly raised aerial tufts made up of disperse groups of monilioid cells present in old cultures; margin diffuse to irregular, partially immersed in the substrate, filiform to moderately lobate. Reverse of the colony whitish-cream at first, then becoming pale orange-cream (2.5
<collectionCode id="ED38AE055125FFBAA75911A5FBBCFA4C" box="[1063,1084,1438,1463]" country="USA" lsid="urn:lsid:biocol.org:col:14792" name="Yale University" pageId="6" pageNumber="181" type="Herbarium">Y</collectionCode>
/8/4) to light ochre (10
<collectionCode id="ED38AE055125FFBAA64D11A5FADDFA4C" box="[1331,1373,1438,1463]" pageId="6" pageNumber="181">YR</collectionCode>
8/6) in older cultures, paler in the centre of the colony.
<emphasis id="B95DEAD25125FFBAA1DA11F9FCCFFA20" box="[676,847,1474,1499]" italics="true" pageId="6" pageNumber="181">Runner hyphae</emphasis>
mostly binucleate (although tri-nucleate elements are also present in older cultures), regularly septate, hyaline, thin-walled, (2.1)2.34.1(4.3) μm in diameter, cylindrical, sometimes laterally bifurcate in right angles and eventually constricted at the base of ramifications, frequently with monilioid-like, terminal finger-like, flexuose to flexuose-nodulose, clavate to fusoid or even subcapitate projections of (3.5)56.5 (9) × (25)3075(80) μm, sometimes with divaricate branching ends.
<emphasis id="B95DEAD25125FFBAA75F1269FB49F990" box="[1057,1225,1618,1643]" italics="true" pageId="6" pageNumber="181">Monilioid cells</emphasis>
binucleate, variable in shape and arrangement, ranging from ellipsoid to subglobose and mostly globose elements, of (1.,9)1215(15.5) μm in diameter, sometimes with apiculate projections, in short and branched (mostly “
<collectionCode id="ED38AE055125FFBAA71512A1FBFEF948" box="[1131,1150,1690,1715]" name="Tavera, Department of Geology and Geophysics" pageId="6" pageNumber="181">T</collectionCode>
” shaped) chains, up to 20 elements, developed in loose clusters forming tufts along the entire colony. Growth rate in
<collectionCode id="ED38AE055125FFBAA7111285FB25F92C" box="[1135,1189,1726,1751]" country="Sri Lanka" httpUri="http://biocol.org/urn:lsid:biocol.org:col:13944" lsid="urn:lsid:biocol.org:col:13944" name="Royal Botanic Gardens" pageId="6" pageNumber="181" type="Herbarium">PDA</collectionCode>
at 28ºC,
<quantity id="4CD19B255125FFBAA6741285FA11F92C" box="[1290,1425,1726,1751]" metricMagnitude="-4" metricUnit="m" metricValue="2.0" metricValueMax="3.0" metricValueMin="1.0" pageId="6" pageNumber="181" unit="mm" value="0.2" valueMax="0.3" valueMin="0.1">0.10.3 mm</quantity>
/h.
<emphasis id="B95DEAD25125FFBAA3F612D9FE96F900" box="[136,278,1762,1787]" italics="true" pageId="6" pageNumber="181">Basidiomata</emphasis>
absent from seedlings samples or not produced after teleomorph-inducing techniques applied.
</paragraph>
</subSubSection>
<subSubSection id="C333654B5125FFBAA3C3133DFCDBF828" pageId="6" pageNumber="181" type="etymology">
<paragraph id="8B9636C05125FFBAA3C3133DFBB7F8B8" blockId="6.[136,1452,1042,2003]" pageId="6" pageNumber="181">
<emphasis id="B95DEAD25125FFBAA3C3133DFEC8F8E4" bold="true" box="[189,328,1798,1823]" pageId="6" pageNumber="181">Etymology:</emphasis>
—The specific epithet refers to the frequent association of this species with the ectomycorrhizal root tips of evergreen oak artificially inoculated with black truffle,
<taxonomicName id="4C294D435125FFBAA03F1311FBB0F8B8" box="[833,1072,1834,1859]" class="Agaricomycetes" family="Paxillaceae" genus="Tuber" kingdom="Fungi" order="Boletales" pageId="6" pageNumber="181" phylum="Basidiomycota" rank="species" species="melanosporum">
<emphasis id="B95DEAD25125FFBAA03F1311FBB0F8B8" box="[833,1072,1834,1859]" italics="true" pageId="6" pageNumber="181">Tuber melanosporum</emphasis>
</taxonomicName>
.
</paragraph>
<paragraph id="8B9636C05125FFBAA3C31375FCDBF828" blockId="6.[136,1452,1042,2003]" pageId="6" pageNumber="181">
<emphasis id="B95DEAD25125FFBAA3C31375FE26F89C" bold="true" box="[189,422,1870,1895]" pageId="6" pageNumber="181">Additional isolates:</emphasis>
—Colonies in PDA (isolates EP1 to EP14) isolated from
<taxonomicName id="4C294D435125FFBAA74A1375FAA4F89C" box="[1076,1316,1870,1895]" class="Agaricomycetes" family="Paxillaceae" genus="Tuber" kingdom="Fungi" order="Boletales" pageId="6" pageNumber="181" phylum="Basidiomycota" rank="species" species="melanosporum">
<emphasis id="B95DEAD25125FFBAA74A1375FAA4F89C" box="[1076,1316,1870,1895]" italics="true" pageId="6" pageNumber="181">Tuber melanosporum</emphasis>
</taxonomicName>
mycorhizal tips coming from 9 one-year old mycorrhizal plants of
<taxonomicName id="4C294D435125FFBAA18E1349FC5FF870" box="[752,991,1906,1931]" class="Magnoliopsida" family="Fagaceae" genus="Quercus" kingdom="Plantae" order="Fagales" pageId="6" pageNumber="181" phylum="Tracheophyta" rank="subSpecies" species="ilex" subSpecies="ballota">
<emphasis id="B95DEAD25125FFBAA18E1349FCBBF870" box="[752,827,1906,1931]" italics="true" pageId="6" pageNumber="181">Q. ilex</emphasis>
subsp.
<emphasis id="B95DEAD25125FFBAA0EE1349FC5FF870" box="[912,991,1906,1931]" italics="true" pageId="6" pageNumber="181">ballota</emphasis>
</taxonomicName>
artificially co-inoculated with a strain of
<taxonomicName id="4C294D435125FFBAA3F613ADFE19F854" box="[136,409,1942,1967]" class="Agaricomycetes" family="Botryobasidiaceae" genus="Trichoderma" kingdom="Fungi" order="Cantharellales" pageId="6" pageNumber="181" phylum="Basidiomycota" rank="species" species="harzianum">
<emphasis id="B95DEAD25125FFBAA3F613ADFE19F854" box="[136,409,1942,1967]" italics="true" pageId="6" pageNumber="181">Trichoderma harzianum</emphasis>
</taxonomicName>
, growing in pots in greenhouse under controlled conditions at the Centro de Investigación y Tecnología Agroalimentaria de Aragón-CITA, Zaragoza (
<collectingCountry id="F33E76505125FFBAA0741381FCD0F828" box="[778,848,1978,2003]" name="Spain" pageId="6" pageNumber="181">Spain</collectingCountry>
).
</paragraph>
</subSubSection>
</treatment>
</document>