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<mods:title id="5454A33E0A12DF61A831DF4B8F07BD38">Phylogenetic position of the diatom genus Geissleria Lange-Bertalot &amp; Metzeltin and description of two new species from Siberian mountain lakes</mods:title>
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<mods:namePart id="3C8C5EA29CFFDD7F44362E150E2C1081">Kulikovskiy, Maxim</mods:namePart>
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<mods:namePart id="D9B077E9DF1119310066A3A90968A9D8">Gusev, Evgeniy</mods:namePart>
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<mods:namePart id="F69BE0E10B67CC075F0E4B3C639067F9">Andreeva, Svetlana</mods:namePart>
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<mods:namePart id="7EF3C8F73DE649E050818B5BDE0DBBD3">Annenkova, Natalia</mods:namePart>
<mods:affiliation id="09306927FB3510A0356AE40EB49411CC">Limnological Institute Siberian Division, Russian Academy of Sciences, Ulan-Batorskaya 3, RUS- 664033, Irkutsk, Russia &amp; Aquatic Ecology, Department of Biology, Lund University, Ecology Building, 22362 Lund, Sweden</mods:affiliation>
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<treatment id="03BB6E293C26D73AFF4BFB86FD9A05E1" ID-DOI="http://doi.org/10.5281/zenodo.5150635" ID-GBIF-Taxon="183841549" ID-Zenodo-Dep="5150635" LSID="urn:lsid:plazi:treatment:03BB6E293C26D73AFF4BFB86FD9A05E1" httpUri="http://treatment.plazi.org/id/03BB6E293C26D73AFF4BFB86FD9A05E1" lastPageNumber="252" pageId="3" pageNumber="252">
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<paragraph id="8BADDF3F3C26D73AFF4BFB86FBC10609" blockId="3.[136,1138,1108,1135]" box="[136,1138,1108,1135]" pageId="3" pageNumber="252">
<heading id="D0E568533C26D73AFF4BFB86FBC10609" box="[136,1138,1108,1135]" fontSize="11" level="2" pageId="3" pageNumber="252" reason="3">
<taxonomicName id="4C12A4BC3C26D73AFF4BFB86FC200609" authority="Kulikovskiy, Gusev, Andreeva &amp; Annenkova" authorityName="Kulikovskiy, Gusev, Andreeva &amp; Annenkova" authorityYear="2014" box="[136,915,1108,1135]" class="Bacillariophyceae" family="Naviculaceae" genus="Geissleria" higherTaxonomySource="GBIF" kingdom="Chromista" order="Naviculales" pageId="3" pageNumber="252" phylum="Ochrophyta" rank="species" species="frolikhiensis" status="sp. nov.">
<emphasis id="B966032D3C26D73AFF4BFB86FE200608" bold="true" box="[136,403,1108,1135]" italics="true" pageId="3" pageNumber="252">Geissleria frolikhiensis</emphasis>
Kulikovskiy, Gusev, Andreeva &amp; Annenkova
</taxonomicName>
<emphasis id="B966032D3C26D73AFC59FB87FC5C0609" box="[922,1007,1109,1134]" italics="true" pageId="3" pageNumber="252">
<taxonomicNameLabel id="A255BE563C26D73AFC59FB87FC5C0609" box="[922,1007,1109,1134]" pageId="3" pageNumber="252" rank="species">sp. nov.</taxonomicNameLabel>
</emphasis>
<figureCitation id="1329C3BA3C26D73AFC36FB86FBC10609" box="[1013,1138,1108,1134]" captionStart="FIGURES 2042" captionStartId="5.[136,243,1216,1238]" captionTargetBox="[151,1435,197,1122]" captionTargetId="figure-17@5.[151,1435,197,1161]" captionTargetPageId="5" captionText="FIGURES 2042. Geissleria frolikhiensis sp. nov. Figs 2042: Figs 2035: Valve view. Figs 3639: Live cells with chloroplast structure, explanation in the text. Fig. 40: External valve view, note biseriate striae near axial area (arrow). Figs 41, 42: Internal valve view, note props in areolae and elongated subpolar pores (annulus). (2039: LM; 4042: SEM). Scale bar for LM: 10 μm." figureDoi="http://doi.org/10.5281/zenodo.5144895" httpUri="https://zenodo.org/record/5144895/files/figure.png" pageId="3" pageNumber="252">Figs 2042</figureCitation>
</heading>
</paragraph>
<paragraph id="8BADDF3F3C26D73AFF4BFB72FEBE06BD" blockId="3.[136,1452,1184,1566]" pageId="3" pageNumber="252">
LM, live cells (
<figureCitation id="1329C3BA3C26D73AFEDDFB72FE2306D1" box="[286,400,1184,1206]" captionStart="FIGURES 2042" captionStartId="5.[136,243,1216,1238]" captionTargetBox="[151,1435,197,1122]" captionTargetId="figure-17@5.[151,1435,197,1161]" captionTargetPageId="5" captionText="FIGURES 2042. Geissleria frolikhiensis sp. nov. Figs 2042: Figs 2035: Valve view. Figs 3639: Live cells with chloroplast structure, explanation in the text. Fig. 40: External valve view, note biseriate striae near axial area (arrow). Figs 41, 42: Internal valve view, note props in areolae and elongated subpolar pores (annulus). (2039: LM; 4042: SEM). Scale bar for LM: 10 μm." figureDoi="http://doi.org/10.5281/zenodo.5144895" httpUri="https://zenodo.org/record/5144895/files/figure.png" pageId="3" pageNumber="252">Figs 3639</figureCitation>
): The single, large chloroplast is divided into two plates lying one against each valve, connected by a broad column.
</paragraph>
</subSubSection>
<subSubSection id="C3088CB43C26D73AFF4BFB3AFD850479" pageId="3" pageNumber="252" type="description">
<paragraph id="8BADDF3F3C26D73AFF4BFB3AFB650746" blockId="3.[136,1452,1184,1566]" pageId="3" pageNumber="252">
LM (
<figureCitation id="1329C3BA3C26D73AFF7BFB3AFE9B0699" box="[184,296,1256,1278]" captionStart="FIGURES 2042" captionStartId="5.[136,243,1216,1238]" captionTargetBox="[151,1435,197,1122]" captionTargetId="figure-17@5.[151,1435,197,1161]" captionTargetPageId="5" captionText="FIGURES 2042. Geissleria frolikhiensis sp. nov. Figs 2042: Figs 2035: Valve view. Figs 3639: Live cells with chloroplast structure, explanation in the text. Fig. 40: External valve view, note biseriate striae near axial area (arrow). Figs 41, 42: Internal valve view, note props in areolae and elongated subpolar pores (annulus). (2039: LM; 4042: SEM). Scale bar for LM: 10 μm." figureDoi="http://doi.org/10.5281/zenodo.5144895" httpUri="https://zenodo.org/record/5144895/files/figure.png" pageId="3" pageNumber="252">Figs 2035</figureCitation>
): Valves elliptical to linear-elliptical with ends obtusely rounded. Length 8.09.5 µm, breadth 5 µm. Raphe filiform.Axial area very narrow, linear. Central area small. Isolated pore lacking. Striae radiate throughout,
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10 µm.
</paragraph>
<paragraph id="8BADDF3F3C26D73AFF4BFAE2FE8307D5" blockId="3.[136,1452,1184,1566]" pageId="3" pageNumber="252">
SEM, external view (
<figureCitation id="1329C3BA3C26D73AFE94FAE2FE120721" box="[343,417,1328,1350]" captionStart="FIGURES 2042" captionStartId="5.[136,243,1216,1238]" captionTargetBox="[151,1435,197,1122]" captionTargetId="figure-17@5.[151,1435,197,1161]" captionTargetPageId="5" captionText="FIGURES 2042. Geissleria frolikhiensis sp. nov. Figs 2042: Figs 2035: Valve view. Figs 3639: Live cells with chloroplast structure, explanation in the text. Fig. 40: External valve view, note biseriate striae near axial area (arrow). Figs 41, 42: Internal valve view, note props in areolae and elongated subpolar pores (annulus). (2039: LM; 4042: SEM). Scale bar for LM: 10 μm." figureDoi="http://doi.org/10.5281/zenodo.5144895" httpUri="https://zenodo.org/record/5144895/files/figure.png" pageId="3" pageNumber="252">Fig. 40</figureCitation>
): The raphe slit is straight with visible sternum. Isolated pore absent. Central raphe ends straight. Terminal raphe ends deflected towards the secondary side of the valve and continue onto mantle. Striae uniseriate or irregularly biseriate near axial area. Annulus structure is not recognizable with striae similar to ones of striae. Areolae present as short slits,
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10 µm of stria length.
</paragraph>
<paragraph id="8BADDF3F3C26D73AFF4BFA12FD850479" blockId="3.[136,1452,1184,1566]" pageId="3" pageNumber="252">
SEM, internal view (
<figureCitation id="1329C3BA3C26D73AFE92FA12FE7107B1" box="[337,450,1472,1494]" captionStart="FIGURES 2042" captionStartId="5.[136,243,1216,1238]" captionTargetBox="[151,1435,197,1122]" captionTargetId="figure-17@5.[151,1435,197,1161]" captionTargetPageId="5" captionText="FIGURES 2042. Geissleria frolikhiensis sp. nov. Figs 2042: Figs 2035: Valve view. Figs 3639: Live cells with chloroplast structure, explanation in the text. Fig. 40: External valve view, note biseriate striae near axial area (arrow). Figs 41, 42: Internal valve view, note props in areolae and elongated subpolar pores (annulus). (2039: LM; 4042: SEM). Scale bar for LM: 10 μm." figureDoi="http://doi.org/10.5281/zenodo.5144895" httpUri="https://zenodo.org/record/5144895/files/figure.png" pageId="3" pageNumber="252">Figs 41, 42</figureCitation>
): Raphe slit lying in internal sternum. Central raphe endings straight, terminal ends terminate in evident helictoglossae. Elongated subpolar areolae (about 4, two of which are striae) with warty outgrowths. Internally no hymens or occlusions are observed on the areolae.
</paragraph>
</subSubSection>
<subSubSection id="C3088CB43C26D73AFF4BF99EFC4104ED" pageId="3" pageNumber="252" type="materials_examined">
<paragraph id="8BADDF3F3C26D73AFF4BF99EFC4104ED" blockId="3.[136,1452,1612,1927]" pageId="3" pageNumber="252">
<emphasis id="B966032D3C26D73AFF4BF99EFF7F0401" bold="true" box="[136,204,1612,1638]" pageId="3" pageNumber="252">Type:</emphasis>
<materialsCitation id="3B7AD5623C26D73AFF2BF99EFC5D04ED" ID-GBIF-Occurrence="3329672301" collectingDate="2012-07-26" collectionCode="IBIW, COLO" collectorName="Lake Frolikha, N &amp; M. Kulikovskiy" country="Russia" location="Lake Frolikha" pageId="3" pageNumber="252" specimenCount="1" typeStatus="holotype">
<collectingCountry id="F3059FAF3C26D73AFF2BF99EFEE30401" box="[232,336,1612,1638]" name="Russia" pageId="3" pageNumber="252">RUSSIA</collectingCountry>
:
<collectorName id="26E7BAE93C26D73AFE9DF99EFD950400" box="[350,550,1612,1639]" pageId="3" pageNumber="252">
<location id="8ECD89E43C26D73AFE9DF99EFDB00401" LSID="urn:lsid:plazi:treatment:03BB6E293C26D73AFF4BFB86FD9A05E1:8ECD89E43C26D73AFE9DF99EFDB00401" box="[350,515,1612,1638]" country="Russia" name="Lake Frolikha" pageId="3" pageNumber="252">Lake Frolikha</location>
, N
</collectorName>
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E
<superScript id="7C6772773C26D73AFD70F99EFC860400" attach="right" box="[691,821,1612,1639]" fontSize="6" pageId="3" pageNumber="252">109058.649</superScript>
,
<date id="FFACF9FF3C26D73AFC80F99EFC770401" box="[835,964,1612,1638]" pageId="3" pageNumber="252" value="2012-07-26">
<collectingDate id="EFE800173C26D73AFC80F99EFC770401" box="[835,964,1612,1638]" pageId="3" pageNumber="252" value="2012-07-26">26.07.2012</collectingDate>
</date>
, leg.
<collectorName id="26E7BAE93C26D73AFBC4F99FFB0F0401" box="[1031,1212,1612,1639]" pageId="3" pageNumber="252">M. Kulikovskiy</collectorName>
(
<collectionCode id="ED0347FA3C26D73AFB0DF99EFABC0401" box="[1230,1295,1612,1638]" country="Russia" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15311" lsid="urn:lsid:biocol.org:col:15311" name="I. D. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences" pageId="3" pageNumber="252" type="Herbarium">IBIW</collectionCode>
slide no. 80/ B369,
<typeStatus id="54A9619D3C26D73AFF10F9A2FE8604ED" box="[211,309,1648,1674]" pageId="3" pageNumber="252" type="holotype">holotype</typeStatus>
designated here (=
<figureCitation id="1329C3BA3C26D73AFDC9F9A2FDEF04ED" box="[522,604,1648,1674]" captionStart="FIGURES 2042" captionStartId="5.[136,243,1216,1238]" captionTargetBox="[151,1435,197,1122]" captionTargetId="figure-17@5.[151,1435,197,1161]" captionTargetPageId="5" captionText="FIGURES 2042. Geissleria frolikhiensis sp. nov. Figs 2042: Figs 2035: Valve view. Figs 3639: Live cells with chloroplast structure, explanation in the text. Fig. 40: External valve view, note biseriate striae near axial area (arrow). Figs 41, 42: Internal valve view, note props in areolae and elongated subpolar pores (annulus). (2039: LM; 4042: SEM). Scale bar for LM: 10 μm." figureDoi="http://doi.org/10.5281/zenodo.5144895" httpUri="https://zenodo.org/record/5144895/files/figure.png" pageId="3" pageNumber="252">Fig. 20</figureCitation>
)),
<collectionCode id="ED0347FA3C26D73AFDB8F9A2FD7404ED" box="[635,711,1648,1674]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:15540" lsid="urn:lsid:biocol.org:col:15540" name="University of Colorado Herbarium" pageId="3" pageNumber="252" type="Herbarium">COLO</collectionCode>
slide 80/B369p is isotype
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="C3088CB43C26D73AFF7EF946FD9A05E1" pageId="3" pageNumber="252" type="etymology">
<paragraph id="8BADDF3F3C26D73AFF7EF946FD6A04C9" blockId="3.[136,1452,1612,1927]" box="[189,729,1684,1711]" pageId="3" pageNumber="252">
<emphasis id="B966032D3C26D73AFF7EF946FEFB04C9" bold="true" box="[189,328,1684,1710]" pageId="3" pageNumber="252">Etymology:</emphasis>
—Epithet refers to the
<typeStatus id="54A9619D3C26D73AFD8BF947FDCB04C8" box="[584,632,1685,1711]" pageId="3" pageNumber="252">type</typeStatus>
locality.
</paragraph>
<paragraph id="8BADDF3F3C26D73AFF7EF96AFD9A05E1" blockId="3.[136,1452,1612,1927]" pageId="3" pageNumber="252">
<emphasis id="B966032D3C26D73AFF7EF96AFED604B5" bold="true" box="[189,357,1720,1746]" pageId="3" pageNumber="252">Observations:</emphasis>
This species is similar to
<taxonomicName id="4C12A4BC3C26D73AFD4CF96AFA1804B4" authority="(Hustedt) Lange-Bertalot &amp; Metzeltin (1996: 64)" authorityName="Lange-Bertalot &amp; Metzeltin" authorityPageNumber="64" authorityYear="1996" baseAuthorityName="Hustedt" box="[655,1451,1720,1747]" class="Bacillariophyceae" family="Naviculaceae" genus="Geissleria" higherTaxonomySource="GBIF" kingdom="Chromista" order="Naviculales" pageId="3" pageNumber="252" phylum="Ochrophyta" rank="species" species="acceptata">
<emphasis id="B966032D3C26D73AFD4CF96AFCC404B5" box="[655,887,1720,1746]" italics="true" pageId="3" pageNumber="252">Geissleria acceptata</emphasis>
(Hustedt)
<bibRefCitation id="EF83A2CE3C26D73AFC32F96AFA1804B4" author="Lange-Bertalot, H. &amp; Metzeltin, D." box="[1009,1451,1720,1747]" pageId="3" pageNumber="252" pagination="1 - 390" refId="ref6930" refString="Lange-Bertalot, H. &amp; Metzeltin, D. (1996) Indicators of oligotrophy - 800 taxa representative of three ecologically distinct lake types, Carbonate buffered - Oligodystrophic - Weakly buffered soft water. Iconographia Diatomologica 2: 1 - 390." type="journal article" year="1996">Lange-Bertalot &amp; Metzeltin (1996: 64)</bibRefCitation>
</taxonomicName>
but differs by rather large valves and more elliptical valves (not linear-elliptical).
<taxonomicName id="4C12A4BC3C26D73AFBE5F90EFB050491" authorityName="Lange-Bertalot &amp; Metzeltin" authorityYear="1996" baseAuthorityName="Hustedt" box="[1062,1206,1756,1782]" class="Bacillariophyceae" family="Naviculaceae" genus="Geissleria" higherTaxonomySource="GBIF" kingdom="Chromista" order="Naviculales" pageId="3" pageNumber="252" phylum="Ochrophyta" rank="species" species="acceptata">
<emphasis id="B966032D3C26D73AFBE5F90EFB050491" box="[1062,1206,1756,1782]" italics="true" pageId="3" pageNumber="252">G. acceptata</emphasis>
</taxonomicName>
possesses an isolated pore, a structure that is absent in
<taxonomicName id="4C12A4BC3C26D73AFDC3F8D2FD1E057D" authority="Kulikovskiy &amp; Gusev &amp; Andreeva &amp; Annenkova, 2014" authorityName="Kulikovskiy &amp; Gusev &amp; Andreeva &amp; Annenkova" authorityYear="2014" box="[512,685,1792,1818]" class="Bacillariophyceae" family="Naviculaceae" genus="Geissleria" higherTaxonomySource="GBIF" kingdom="Chromista" order="Naviculales" pageId="3" pageNumber="252" phylum="Ochrophyta" rank="species" species="frolikhiensis" status="sp. nov.">
<emphasis id="B966032D3C26D73AFDC3F8D2FD1E057D" box="[512,685,1792,1818]" italics="true" pageId="3" pageNumber="252">G. frolikhiensis</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A255BE563C26D73AFD76F8D3FCBF057C" box="[693,780,1793,1819]" pageId="3" pageNumber="252" rank="species">sp. nov.</taxonomicNameLabel>
(
<bibRefCitation id="EF83A2CE3C26D73AFCD8F8D2FC41057D" author="Novais, M. H. &amp; Wetzel, C. E. &amp; Van de Vijver, B. &amp; Morais, M. M. &amp; Hoffmann, L. &amp; Ector L." box="[795,1010,1792,1818]" pageId="3" pageNumber="252" pagination="117 - 148" refId="ref7094" refString="Novais, M. H., Wetzel, C. E., Van de Vijver, B., Morais, M. M., Hoffmann, L. &amp; Ector L. (2013) New species and combinations in the genus Geissleria (Bacilllariophyceae). Cryptogamie, Algologie 34 (2): 117 - 148. http: // dx. doi. org / 10.7872 / crya. v 34. iss 2.2013.117" type="journal article" year="2013">
Novais
<emphasis id="B966032D3C26D73AFCB7F8D3FC1D057D" box="[884,942,1792,1818]" italics="true" pageId="3" pageNumber="252">et al.</emphasis>
2013
</bibRefCitation>
). Other similar species are absent from Europe or elsewhere (see
<bibRefCitation id="EF83A2CE3C26D73AFE76F8F6FD100559" author="Lange-Bertalot, H." box="[437,675,1828,1854]" pageId="3" pageNumber="252" pagination="1 - 526" refId="ref6900" refString="Lange-Bertalot, H. (2001) Navicula sensu stricto. 10 genera separated from Navicula sensu lato, Frustulia. Diatom of Europe 2: 1 - 526." type="journal article" year="2001">Lange-Bertalot 2001</bibRefCitation>
,
<bibRefCitation id="EF83A2CE3C26D73AFD72F8F6FC3F0559" author="Novais, M. H. &amp; Wetzel, C. E. &amp; Van de Vijver, B. &amp; Morais, M. M. &amp; Hoffmann, L. &amp; Ector L." box="[689,908,1828,1854]" pageId="3" pageNumber="252" pagination="117 - 148" refId="ref7094" refString="Novais, M. H., Wetzel, C. E., Van de Vijver, B., Morais, M. M., Hoffmann, L. &amp; Ector L. (2013) New species and combinations in the genus Geissleria (Bacilllariophyceae). Cryptogamie, Algologie 34 (2): 117 - 148. http: // dx. doi. org / 10.7872 / crya. v 34. iss 2.2013.117" type="journal article" year="2013">
Novais
<emphasis id="B966032D3C26D73AFCC9F8F7FCF50559" box="[778,838,1828,1854]" italics="true" pageId="3" pageNumber="252">et al.</emphasis>
2013
</bibRefCitation>
).
<taxonomicName id="4C12A4BC3C26D73AFC62F8F6FE320505" authority="Kulikovskiy, Lange-Bertalot &amp; Metzeltin (2012: 117)" authorityName="Kulikovskiy, Lange-Bertalot &amp; Metzeltin" authorityPageNumber="117" authorityYear="2012" class="Bacillariophyceae" family="Naviculaceae" genus="Geissleria" higherTaxonomySource="GBIF" kingdom="Chromista" order="Naviculales" pageId="3" pageNumber="252" phylum="Ochrophyta" rank="species" species="irregularis">
<emphasis id="B966032D3C26D73AFC62F8F6FB8D0559" box="[929,1086,1828,1854]" italics="true" pageId="3" pageNumber="252">G. irregularis</emphasis>
<bibRefCitation id="EF83A2CE3C26D73AFB8AF8F6FE320505" author="Kulikovskiy, M. S. &amp; Lange-Bertalot, H. &amp; Metzeltin, D. &amp; Witkowski, A." pageId="3" pageNumber="252" pagination="7 - 608" refId="ref6581" refString="Kulikovskiy, M. S., Lange-Bertalot, H., Metzeltin, D. &amp; Witkowski, A. (2012 a) Lake Baikal: hotspot of endemic diatoms I. Iconographia Diatomologica, V. 23, A. R. G. Gantner Verlag K. G., pp. 7 - 608." type="book chapter" year="2012">Kulikovskiy, Lange-Bertalot &amp; Metzeltin (2012: 117)</bibRefCitation>
</taxonomicName>
from Lake Baikal differs by asymmetrical valves to the apical axis, central area. No similar species are known from Lake Baikal.
</paragraph>
</subSubSection>
</treatment>
</document>