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<document id="1F33EEBDCC35208E8916DFCB580FA6DF" ID-DOI="10.3897/mycokeys.107.125549" ID-publisher-id="125549" URI-arpha="3EA0E723-E6FC-58B0-8491-ED4CC2FBAB87" XM.bibliography_approvedBy="admin" XM.materialsCitations_approvedBy="admin" XM.taxonomicNames_approvedBy="admin" XM.treatmentCitations_approvedBy="admin" XM.treatments_approvedBy="admin" article-type="research-article" checkinTime="1723223129036" checkinUser="pensoft" docAuthor="Tedersoo, Leho, Magurno, Franco, Alkahtani, Saad &amp; Mikryukov, Vladimir" docDate="2024" docId="4E91B05A4FD25067B51FB76F2A2DE7F7" docLanguage="en" docName="MycoKeys 107: 249-271" docOrigin="MycoKeys 107" docSource="https://mycokeys.pensoft.net/article/125549/download/xml/" docStyle="DocumentStyle:PensoftTaxPub.0000.journal_article.mycokeys" docStyleName="PensoftTaxPub.0000.journal_article.mycokeys" docTitle="Kungsaengena shadiae Tedersoo 2024, sp. nov." docType="treatment" docVersion="3" dtd-version="3.0" lastPageNumber="271" masterDocId="3EA0E723E6FC58B08491ED4CC2FBAB87" masterDocTitle="Phylogenetic classification of arbuscular mycorrhizal fungi: new species and higher-ranking taxa in Glomeromycota and Mucoromycota (class Endogonomycetes)" masterLastPageNumber="271" masterPageNumber="249" pageNumber="249" updateTime="1723229325626" updateUser="admin">
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<mods:titleInfo id="87E6888D6F9D939E9ED24FBCAE503BFF">
<mods:title id="EEAC15454FB5190E19D5CC8748F0B057">Phylogenetic classification of arbuscular mycorrhizal fungi: new species and higher-ranking taxa in Glomeromycota and Mucoromycota (class Endogonomycetes)</mods:title>
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<mods:name id="471DD30D78F328F75BBE5AF0ACEF95E3" type="personal">
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<mods:namePart id="D51B7E472AE8BCB610898FB0B626E699">Tedersoo, Leho</mods:namePart>
<mods:affiliation id="6BF81BF80C708094FFE58EA2E121F8D3">Mycology and Microbiology Center, University of Tartu, 2 Liivi, 50409 Tartu, Estonia &amp; Department of Zoology, College of Science, King Saud University, 12371 Riyadh, Saudi Arabia</mods:affiliation>
</mods:name>
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<mods:namePart id="DAC41AE2F0CEF0096D4C3BED6E8CA65F">Magurno, Franco</mods:namePart>
<mods:nameIdentifier id="13E527D1E84700CD2BD37DA7BF8656AC" type="ORCID">0000-0002-3117-8149</mods:nameIdentifier>
<mods:affiliation id="5DA372B08FE68593CA5D3A1984F470B1">Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40 - 032 Katowice, Poland</mods:affiliation>
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<mods:namePart id="44ACE0ADE6D107A297BFC594177317F0">Alkahtani, Saad</mods:namePart>
<mods:nameIdentifier id="7D1A5417F5BD73D7E45D679A34234516" type="ORCID">0000-0001-7381-5110</mods:nameIdentifier>
<mods:affiliation id="87B96D4A1992669BC16481279B7B8DD7">Department of Zoology, College of Science, King Saud University, 12371 Riyadh, Saudi Arabia</mods:affiliation>
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<mods:namePart id="C9BFC1CEAF1CB7AF3946A373C7711E78">Mikryukov, Vladimir</mods:namePart>
<mods:nameIdentifier id="2C1A9B2989CA174022ED2C7BD59C40C6" type="ORCID">0000-0003-2786-2690</mods:nameIdentifier>
<mods:affiliation id="D9842BE9F44050CD3EB17232E3DAA146">Mycology and Microbiology Center, University of Tartu, 2 Liivi, 50409 Tartu, Estonia</mods:affiliation>
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<mods:typeOfResource id="30EF91A746CEEC1BE8B90B72D74076F9">text</mods:typeOfResource>
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<mods:title id="144AA5E7CDA2E66D5F0D724DB9D323F0">MycoKeys</mods:title>
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<mods:date id="D0D0357C10A7E91A981EA4AC65C1C3E2">2024</mods:date>
<mods:detail id="91ACAB94B3E6E7B6EC96167AAF907B39" type="pubDate">
<mods:number id="E9934B92ED1A700C120E910BFE1D2582">2024-08-09</mods:number>
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<mods:number id="5E64A6EB953ADD3AC042FD0D6A983667">107</mods:number>
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<mods:classification id="EF1D543E53A79EC6555D1D3C0A10C672">journal article</mods:classification>
<mods:identifier id="FB4CA2F19E28DA78BCBC3DDA4A9ED317" type="DOI">10.3897/mycokeys.107.125549</mods:identifier>
</mods:mods>
<treatment id="4E91B05A4FD25067B51FB76F2A2DE7F7" ID-DOI="http://doi.org/10.5281/zenodo.13286504" ID-Zenodo-Dep="13286504" ID-arpha="4E91B05A-4FD2-5067-B51F-B76F2A2DE7F7" ID-mycobank="853583" LSID="urn:lsid:plazi:treatment:4E91B05A4FD25067B51FB76F2A2DE7F7" httpUri="http://treatment.plazi.org/id/4E91B05A4FD25067B51FB76F2A2DE7F7">
<subSubSection id="BEEE5E1084F5D3A1B48F8D0DFA18AF21" type="nomenclature">
<paragraph id="29EACDEF408BADEE52488E27F6DA8113">
<taxonomicName id="0EA39076C718BFE86FF09D06659BF80D" authority="Tedersoo" authorityName="Tedersoo" authorityYear="2024" class="Magnoliopsida" family="Kungsaengenaceae" genus="Kungsaengena" kingdom="Fungi" order="Kungsaengenales" phylum="Tracheophyta" rank="species" species="shadiae" status="sp. nov.">
<emphasis id="5A6FCB0B0E9DCEB928BA72C898973FFE" italics="true">Kungsaengena shadiae</emphasis>
Tedersoo
</taxonomicName>
<taxonomicNameLabel id="5AD16B4FEFDBF1B5FBAB42082BB57853" rank="species">sp. nov.</taxonomicNameLabel>
</paragraph>
</subSubSection>
<subSubSection id="SECID0EY3BI" type="diagnosis">
<paragraph id="630EA3A7C8126C578F554F2F51F544D3">
<heading id="C263B25DF7F8102A0B2C1467FF1AAD7B" reason="title">Diagnosis.</heading>
</paragraph>
<paragraph id="B1665B8396307A1699D39F349424D30D">
separation from other species of
<taxonomicName id="1ECA517AA264BC7BE181C76BE1263AC5" class="Magnoliopsida" family="Dipterocarpaceae" genus="Kungsaengena" kingdom="Plantae" order="Malvales" phylum="Tracheophyta" rank="genus">
<emphasis id="224E96D2A405613B3B66E67E4F6E805A" italics="true">Kungsaengena</emphasis>
</taxonomicName>
based on the
<abbrev id="ABBRID0EF4BI" xlink_title="internal transcribed spacer">ITS</abbrev>
region (ITS 2 positions 2544 tgggaacccatttcgtcgga; one mismatch allowed) and LSU (positions 665694 cgttggggctgggacgcccgtcgctcgcac; one mismatch allowed) as indicated in Fig.
<figureCitation id="66866D517C56561635D741BB283D56F7" captionStart="Figure 7" captionStartId="F7" captionText="Figure 7. Diagnostic barcodes for Kungsaengena shadiae relative to closely-related taxa in ITS 2 and LSU." figureDoi="10.3897/mycokeys.107.125549.figure7" httpUri="https://binary.pensoft.net/fig/1111394">7</figureCitation>
.
</paragraph>
<caption id="E0DB5DE119E9E5A828D516F1BFFBC5C1" ID-DOI="10.3897/mycokeys.107.125549.figure7" ID-arpha="F98448CE-5B6C-5F97-8C36-6B08013F35FD" httpUri="https://binary.pensoft.net/fig/1111394" startId="F7">
<paragraph id="2689A5309D8940B840550C64D2877F52">
<label id="6FDBA814B81A90B01D896FD6B78106C1">Figure 7.</label>
</paragraph>
<paragraph id="84A6D6E69A3E531FA1937EFA7BF0BE56">
Diagnostic barcodes for
<taxonomicName id="4F304C8DB14F1EC51F3CB21D4EE14847" class="Magnoliopsida" family="Dipterocarpaceae" genus="Kungsaengena" kingdom="Plantae" order="Malvales" phylum="Tracheophyta" rank="species" species="shadiae">
<emphasis id="A7AA22F632B2B7BD8E3908D00DF24E45" italics="true">Kungsaengena shadiae</emphasis>
</taxonomicName>
relative to closely-related taxa in ITS 2 and LSU.
</paragraph>
</caption>
</subSubSection>
<subSubSection id="SECID0EF5BI" type="material">
<paragraph id="C37B0CE2A7EB455AFD7F916166A862DC">
<heading id="2C9472B92DAD316BFA6C5B9B76B140A1" reason="title">Type.</heading>
</paragraph>
<paragraph id="5131572E32140B95B0E8CB97B70C6C73">
<materialsCitation id="CB6EE2DD07B71D90988CA84D017423B7" collectedFrom="wet grassland" collectionCode="TUE, EUK" country="Estonia" latitude="58.35555" location="Haage" longLatPrecision="1" longitude="26.61277" specimenCode="TUE 128324, EUK 1603402, TUE 028324" specimenCount="2" typeStatus="holotype">
<abbrev id="ABBRID0EL5BI" xlink_title="environmental DNA">eDNA</abbrev>
sample
<specimenCode id="2265D6F146A10D57D8431381AE04493D">TUE 128324</specimenCode>
(
<emphasis id="A5B2698DA21231E30D6E05F6D2347F2F" bold="true" italics="true">
<typeStatus id="B587DC5505571DF1D7C5CB5089EA449C" type="holotype">holotype</typeStatus>
</emphasis>
);
<abbrev id="ABBRID0ES5BI" xlink_title="environmental DNA">eDNA</abbrev>
sequence
<specimenCode id="B96986B7FCA572F463AF87E328DE3495">EUK 1603402</specimenCode>
(
<emphasis id="CDEB75691384BD3B50A130859021CF37" bold="true" italics="true">
<typeStatus id="27CAB84BC4617D7E3F95E26526D421D8" type="lectotype">lectotype</typeStatus>
</emphasis>
);
<abbrev id="ABBRID0EZ5BI" xlink_title="Global Soil Mycobiome consortium">GSMc</abbrev>
plot G 5763,
<collectedFrom id="FE4E5A9F33B6991DE1FF082FC04DFEC1">wet grassland</collectedFrom>
(soil sample
<specimenCode id="9C9BFCFD847780BCD1909126C6371FF2">TUE 028324</specimenCode>
) in
<location id="C332FB74C0B2AF2F7A45630CF9E97044" LSID="urn:lsid:plazi:treatment:4E91B05A4FD25067B51FB76F2A2DE7F7:C332FB74C0B2AF2F7A45630CF9E97044" country="Estonia" latitude="58.35555" longLatPrecision="1" longitude="26.61277" name="Haage">Haage</location>
,
<collectingCountry id="C695619EB59DE563D91786DEDED5181C" name="Estonia">Estonia</collectingCountry>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0EA6BI" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[26.612770,58.355550]}">
<geoCoordinate id="EB3CF456D3B0142BF82616621B64052F" degrees="58.35555" direction="north" orientation="latitude" precision="1" value="58.35555">58.35555 ° N</geoCoordinate>
,
<geoCoordinate id="C3BF4B42EBB9317DD723CDC6CFB1C77B" degrees="26.61277" direction="east" orientation="longitude" precision="1" value="26.61277">26.61277 ° E</geoCoordinate>
</named-content>
)
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="SECID0EF6BI" type="description">
<paragraph id="75CDAF8A59C343C022EA950E996B6BF8">
<heading id="D555630CAEDFEC8934024395FFC2B38C" reason="title">Description.</heading>
</paragraph>
<paragraph id="3A0C6E9D22ECEF363676E49CFC2B494D">
other sequences: EUK 1604022 (
<abbrev id="ABBRID0EL6BI" xlink_title="Global Soil Mycobiome consortium">GSMc</abbrev>
plot G 5906, football field soil in Karksi-Nuia,
<collectingCountry id="B02456841F6E32EEF5A0042184187555" name="Estonia">Estonia</collectingCountry>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0ES6BI" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[25.559590,58.100880]}">
<geoCoordinate id="39A1D080416041431163383D63543DDF" degrees="58.10088" direction="north" orientation="latitude" precision="1" value="58.10088">58.10088 ° N</geoCoordinate>
,
<geoCoordinate id="CC88921F097331DA26CE6C07522E3F04" degrees="25.55959" direction="east" orientation="longitude" precision="1" value="25.55959">25.55959 ° E</geoCoordinate>
</named-content>
); EUK 1604023 (
<abbrev id="ABBRID0EX6BI" xlink_title="Global Soil Mycobiome consortium">GSMc</abbrev>
plot G 5844, wet pasture soil in Tuhala,
<collectingCountry id="7DA17979A6E93184B70D5298D96CBE8F" name="Estonia">Estonia</collectingCountry>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0E56BI" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[25.002830,59.230030]}">
<geoCoordinate id="8E7C9273AFD956E56AFDF797BD4F52E5" degrees="59.23003" direction="north" orientation="latitude" precision="1" value="59.23003">59.23003 ° N</geoCoordinate>
,
<geoCoordinate id="F1EE0DA743E3786103B6EC940B88E654" degrees="25.00283" direction="east" orientation="longitude" precision="1" value="25.00283">25.00283 ° E</geoCoordinate>
</named-content>
); EUK 1604025 (
<abbrev id="ABBRID0EDACI" xlink_title="Global Soil Mycobiome consortium">GSMc</abbrev>
plot G 4444,
<collectingCountry id="3C585DDBE963DD80E6A9751452C1E8AA" name="Estonia">Estonia</collectingCountry>
, mixed forest soil in Altnurga,
<collectingCountry id="14C9001A407D2D266DBBC4405275701F" name="Estonia">Estonia</collectingCountry>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0EKACI" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[26.283210,58.536760]}">
<geoCoordinate id="ECD7B0EBA78DDCCF5C97E5342856886C" degrees="58.53676" direction="north" orientation="latitude" precision="1" value="58.53676">58.53676 ° N</geoCoordinate>
,
<geoCoordinate id="B6212567788F91868E1664649B8777E0" degrees="26.28321" direction="east" orientation="longitude" precision="1" value="26.28321">26.28321 ° E</geoCoordinate>
</named-content>
); and
<ext-link id="D507E427532E0CC49048440C858CF294" ext-link-type="gen" xlink_href="OU942286" xlink_type="simple">OU 942286</ext-link>
(grassland soil in Kungsängen,
<collectingCountry id="2AF34BC341B542A126E1B54745C9F906" name="Sweden">Sweden</collectingCountry>
,
<named-content content-type="dwc:verbatimCoordinates" id="NCID0EXACI" specific-use="{&quot;type&quot;:&quot;Point&quot;,&quot;coordinates&quot;:[17.661000,59.837000]}">
<geoCoordinate id="9F4C806D177B09B7022D1AA54C6782F5" degrees="59.837" direction="north" orientation="latitude" precision="55" value="59.837">59.837 ° N</geoCoordinate>
,
<geoCoordinate id="AB160248565E2F429264F78AB2F13A63" degrees="17.661" direction="east" orientation="longitude" precision="55" value="17.661">17.661 ° E</geoCoordinate>
</named-content>
), isolated by Shadi Eshghi Sahraei (
<bibRefCitation id="131748C3D7BB144FE27BC325CBC004CA" DOI="10.1002/ece3.8676" author="Eshghi Sahraei" etAl="et al." firstAuthor="Eshghi Sahraei" refId="B25" refString="Eshghi Sahraei S, Furneaux B, Kluting K, Zakieh M, Rydin H, Hytteborn H, Rosling A (2022) Effects of operational taxonomic unit inference methods on soil microeukaryote community analysis using long-read metabarcoding. Ecology and Evolution 12 (3): e 8676. https://doi.org/10.1002/ece3.8676" year="2022">Eshghi Sahraei et al. 2022</bibRefCitation>
).
</paragraph>
</subSubSection>
<subSubSection id="SECID0EGBCI" type="etymology">
<paragraph id="BA169EC58D4F3E8C5D999E4C542DDA74">
<heading id="E2EF222D3E27741563A01628079A69AB" reason="title">Etymology.</heading>
</paragraph>
<paragraph id="6007D282F4BDEF5950E965942FD4DD3E">
<emphasis id="8FB715DC62DCF447694A4AD6DC11E4D7" italics="true">Kungsängen</emphasis>
(Swedish) refers to type locality; and
<emphasis id="2166DCE4661D73871EE6AAECF4A16DFC" italics="true">Shadi</emphasis>
(Persian) refers to the first name of Shadi Eshghi Sahraei who analysed materials collected from the type locality.
</paragraph>
</subSubSection>
<subSubSection id="SECID0E2BCI" type="notes">
<paragraph id="9B704600660BE315AF253656CC1D62D5">
<heading id="746526BA2E37FA9654B2C7301E4A7111" reason="title">Notes.</heading>
</paragraph>
<paragraph id="6DB550A50D69C01499DA53F233C52AF1">
Found from the Baltic States and
<collectingCountry id="5E5BD4B8D47AC0680ABCE3D9ACC4D36C" name="Sweden">Sweden</collectingCountry>
, with
<abbrev id="ABBRID0EBCCI" xlink_title="internal transcribed spacer">ITS</abbrev>
and LSU sequences differing up to 15 % and 1 %, respectively. The
<abbrev id="ABBRID0EFCCI" xlink_title="internal transcribed spacer">ITS</abbrev>
region is infested with microsatellite-like regions and homopolymers, and many sequence variants have long deletions in multiple positions.
<taxonomicName id="78811596C612EBE9D308D27C76B7791C" class="Magnoliopsida" family="Dipterocarpaceae" genus="Kungsaengena" kingdom="Plantae" order="Malvales" phylum="Tracheophyta" rank="species" species="shadiae">
<emphasis id="7137B8718D69F5C429127A918AE712C3" italics="true">K. shadiae</emphasis>
</taxonomicName>
seems to be generalist in terms of habitat type.
</paragraph>
</subSubSection>
</treatment>
</document>