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<document id="313F2DCD7A56B955118AC5CAF0A5A10E" ID-CLB-Dataset="269412" ID-DOI="10.4467/16890027AP.15.003.2190" ID-GBIF-Dataset="dfc981c6-93a7-4e20-b6d4-8520d8f93ce0" ID-ISSN="1689-0027" ID-Zenodo-Dep="8356852" IM.bibliography_approvedBy="diego" IM.illustrations_approvedBy="diego" IM.tables_approvedBy="diego" IM.treatments_approvedBy="diego" checkinTime="1695063305810" checkinUser="felipe" docAuthor="Pan, Xuming, Huang, Jie, Fan, Xinpeng, Ma, Honggang, Al-Rasheid, Khaled A. S., Miao, Miao &amp; Gao, Feng" docDate="2015" docId="03B9BA0AFFB1FFF1FCC03C45FAB31C14" docLanguage="en" docName="ActaProtozool.54.1.31-43.pdf" docOrigin="Acta Protozoologica 54 (1)" docSource="https://www.mendeley.com/catalogue/322bdcf8-809d-3b96-97f8-c8858a43d243/" docStyle="DocumentStyle:64ABA4BEA387EE5C24A0DDEB32CC92E7.2:ActaProtozool.2014-.journal_article" docStyleId="64ABA4BEA387EE5C24A0DDEB32CC92E7" docStyleName="ActaProtozool.2014-.journal_article" docStyleVersion="2" docTitle="Pleuronema elegans Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao 2015, spec. nov." docType="treatment" docVersion="4" lastPageNumber="36" masterDocId="FF80C272FFB0FFF4FFE93B43FFE91D09" masterDocTitle="Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov." masterLastPageNumber="43" masterPageNumber="31" pageNumber="32" updateTime="1695667487477" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="52F2F239C36A87343D2F32A14705D630">Morphology and Phylogeny of Four Marine Scuticociliates (Protista, Ciliophora), with Descriptions of Two New Species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov.</mods:title>
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<mods:namePart id="7465EDF277CB41867799EE6A5AAA31EC">Pan, Xuming</mods:namePart>
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<mods:namePart id="FFAEDD6289747EE821F4DF8D30ACAE1E">Huang, Jie</mods:namePart>
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<mods:namePart id="F8ED465A68342A3B708664DD9E9BCF3F">Fan, Xinpeng</mods:namePart>
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<mods:namePart id="8C320D01B48CD67BE5876FF95824DA7D">Ma, Honggang</mods:namePart>
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<mods:namePart id="387D8FAFF77DAF29EA4044CA120222C3">Al-Rasheid, Khaled A. S.</mods:namePart>
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<mods:namePart id="A3C0B7CCAB5CE5BBA12F7DFA05ECA2C6">Miao, Miao</mods:namePart>
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<mods:namePart id="97E27FA515A21BFD712BD66F578E640C">Gao, Feng</mods:namePart>
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<treatment id="03B9BA0AFFB1FFF1FCC03C45FAB31C14" ID-DOI="http://doi.org/10.5281/zenodo.8377645" ID-Zenodo-Dep="8377645" LSID="urn:lsid:plazi:treatment:03B9BA0AFFB1FFF1FCC03C45FAB31C14" httpUri="http://treatment.plazi.org/id/03B9BA0AFFB1FFF1FCC03C45FAB31C14" lastPageId="5" lastPageNumber="36" pageId="1" pageNumber="32">
<subSubSection id="C30A5897FFB1FFF5FCC03C45FA821A29" box="[809,1387,1798,1825]" pageId="1" pageNumber="32" type="nomenclature">
<paragraph id="8BAF0B1CFFB1FFF5FCC03C45FA821A29" blockId="1.[809,1387,1727,1825]" box="[809,1387,1798,1825]" pageId="1" pageNumber="32">
<heading id="D0E7BC70FFB1FFF5FCC03C45FA821A29" bold="true" box="[809,1387,1798,1825]" fontSize="11" level="2" pageId="1" pageNumber="32" reason="4">
<emphasis id="B964D70EFFB1FFF5FCC03C45FA821A29" bold="true" box="[809,1387,1798,1825]" pageId="1" pageNumber="32">
<taxonomicName id="4C10709FFFB1FFF5FCC03C45FBF61A28" authority="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao, 2015" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[809,1055,1798,1825]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="1" pageNumber="32" phylum="Ciliophora" rank="species" species="elegans" status="spec. nov.">
<emphasis id="B964D70EFFB1FFF5FCC03C45FBF61A28" bold="true" box="[809,1055,1798,1825]" italics="true" pageId="1" pageNumber="32">Pleuronema elegans</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A2576A75FFB1FFF5FBCE3C45FB491A28" box="[1063,1184,1798,1825]" pageId="1" pageNumber="32" rank="species">spec. nov.</taxonomicNameLabel>
(
<figureCitation id="132B1799FFB1FFF5FB593C45FB101A29" box="[1200,1273,1798,1824]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="1" pageNumber="32">Fig. 2</figureCitation>
,
<tableCitation id="C6923EA7FFB1FFF5FAEF3C45FA891A29" box="[1286,1376,1798,1824]" captionStart="Table 1" captionStartId="4.[132,188,223,244]" captionTargetPageId="4" captionText="Table 1. Morphometric characterization of Pleuronema elegans spec. nov. (upper row), P. setigerum Calkins, 1902 (second row), P. grolierei Wang et al., 2008 (third row) and Uronema orientalis spec. nov. (lower row). Data based on protargol stained specimens.All measurements in µm. Abbreviations: CV coefficient of variation in %; Max maximum; Mean arithmetic mean; Min minimum; n number of individuals examined; SD standard deviation." httpUri="http://table.plazi.org/id/DF6F5B94FFB4FFF0FF6D3B9CFD8E1C40" pageId="1" pageNumber="32" tableUuid="DF6F5B94FFB4FFF0FF6D3B9CFD8E1C40">Table 1</tableCitation>
)
</emphasis>
</heading>
</paragraph>
</subSubSection>
<subSubSection id="C30A5897FFB1FFF6FCA63C7BFE7F1B1D" lastPageId="2" lastPageNumber="33" pageId="1" pageNumber="32" type="diagnosis">
<paragraph id="8BAF0B1CFFB1FFF6FCA63C7BFE7F1B1D" blockId="1.[809,1453,1848,1945]" lastBlockId="2.[132,779,1391,1903]" lastPageId="2" lastPageNumber="33" pageId="1" pageNumber="32">
<emphasis id="B964D70EFFB1FFF5FCA63C7BFC381A5B" bold="true" box="[847,977,1848,1874]" pageId="1" pageNumber="32">Diagnosis:</emphasis>
Size
<emphasis id="B964D70EFFB1FFF5FBF03C7AFB821A5B" box="[1049,1131,1849,1874]" italics="true" pageId="1" pageNumber="32">in vivo</emphasis>
90115 × 4560 µm with a distinctly pointed posterior end; contractile vacuole located dorsally near posterior end; about 10 prolonged caudal cilia; consistently two preoral and 18 or 19 somatic kineties; membranelle 1 with a length about 50% that of the anterior part of membranelle 2 which is double-rowed with its posterior end straight but not hook-shaped; marine habitat.
</paragraph>
</subSubSection>
<caption id="DF6F5B94FFB2FFF6FF6D3FCFFF0519FF" ID-DOI="http://doi.org/10.5281/zenodo.8356854" ID-Zenodo-Dep="8356854" httpUri="https://zenodo.org/record/8356854/files/figure.png" pageId="2" pageNumber="33" startId="2.[132,167,1164,1186]" targetBox="[352,1239,243,1116]" targetPageId="2" targetType="figure">
<paragraph id="8BAF0B1CFFB2FFF6FF6D3FCFFF0519FF" blockId="2.[132,1463,1163,1270]" pageId="2" pageNumber="33">
<emphasis id="B964D70EFFB2FFF6FF6D3FCFFF2F19A8" bold="true" box="[132,198,1164,1186]" pageId="2" pageNumber="33">Fig. 1.</emphasis>
Map and photographs of biotopes (AD) in which the samples were collected.
<emphasis id="B964D70EFFB2FFF6FC093FCFFC1819A8" bold="true" box="[992,1009,1164,1185]" pageId="2" pageNumber="33">A</emphasis>
no. 1 swimming beach, Qingdao (36°06N; 120°32E);
<emphasis id="B964D70EFFB2FFF6FF1A3FEAFEEA19B7" bold="true" box="[243,259,1193,1214]" pageId="2" pageNumber="33">B</emphasis>
beach near Sculpture Garden, Qingdao (36°4N; 120°29E);
<emphasis id="B964D70EFFB2FFF6FCB73FEBFC8619B7" bold="true" box="[862,879,1192,1214]" pageId="2" pageNumber="33">C</emphasis>
coastal area of no. 1 swimming beach, Qingdao (36°06N; 120°32E), the site that is further into the sea than that of (A);
<emphasis id="B964D70EFFB2FFF6FD123F86FCE519D3" bold="true" box="[763,780,1221,1242]" pageId="2" pageNumber="33">D</emphasis>
a mangrove wetland in Shenzhen, Guangdong Province (22°30N; 114°37E).
</paragraph>
</caption>
<subSubSection id="C30A5897FFB2FFF6FF433D5EFD081A20" pageId="2" pageNumber="33" type="materials_examined">
<paragraph id="8BAF0B1CFFB2FFF6FF433D5EFE221B53" blockId="2.[132,779,1391,1903]" pageId="2" pageNumber="33">
<materialsCitation id="3B780141FFB2FFF6FF433D5EFE2E1B53" country="China" latitude="36.1" location="Swimming" longLatPrecision="1189" longitude="120.53333" municipality="Qingdao" pageId="2" pageNumber="33" specimenCount="1" typeStatus="holotype">
<emphasis id="B964D70EFFB2FFF6FF433D5EFEBA1B3F" bold="true" box="[170,339,1564,1591]" pageId="2" pageNumber="33">
<typeStatus id="54ABB5BEFFB2FFF6FF433D5EFF0E1B3E" box="[170,231,1565,1591]" pageId="2" pageNumber="33">Type</typeStatus>
locality:
</emphasis>
<location id="8ECF5DC7FFB2FFF6FEB03D5FFE331B3F" LSID="urn:lsid:plazi:treatment:03B9BA0AFFB1FFF1FCC03C45FAB31C14:8ECF5DC7FFB2FFF6FEB03D5FFE331B3F" box="[345,474,1564,1590]" country="China" latitude="36.1" longLatPrecision="1189" longitude="120.53333" municipality="Qingdao" name="Swimming" pageId="2" pageNumber="33">Swimming</location>
beach,
<collectingMunicipality id="6BCB9166FFB2FFF6FDDD3D5FFD751B3F" box="[564,668,1564,1590]" pageId="2" pageNumber="33">Qingdao</collectingMunicipality>
, northern
<collectingCountry id="F3074B8CFFB2FFF6FF6D3D7CFF231B50" box="[132,202,1599,1625]" name="China" pageId="2" pageNumber="33">China</collectingCountry>
(
<geoCoordinate id="EE246DDBFFB2FFF6FF333D7DFEA91B53" box="[218,320,1598,1626]" degrees="36" direction="north" minutes="06" orientation="latitude" pageId="2" pageNumber="33" precision="925" value="36.1">36°06N</geoCoordinate>
;
<geoCoordinate id="EE246DDBFFB2FFF6FEA53D7DFE561B53" box="[332,447,1598,1626]" degrees="120" direction="east" minutes="32" orientation="longitude" pageId="2" pageNumber="33" precision="925" value="120.53333">120°32E</geoCoordinate>
)
</materialsCitation>
.
</paragraph>
<paragraph id="8BAF0B1CFFB2FFF6FF433D21FD081A20" blockId="2.[132,779,1391,1903]" pageId="2" pageNumber="33">
<emphasis id="B964D70EFFB2FFF6FF433D21FEA71B75" bold="true" box="[170,334,1634,1660]" pageId="2" pageNumber="33">Type slides:</emphasis>
<materialsCitation id="3B780141FFB2FFF6FE8F3D21FD341A20" collectionCode="NHMUK, OUC" country="China" county="London" location="Laboratory of Protozoology" municipality="Natural History Museum" pageId="2" pageNumber="33" specimenCount="1" typeStatus="holotype">
The
<typeStatus id="54ABB5BEFFB2FFF6FE453D21FDFA1B75" box="[428,531,1634,1660]" pageId="2" pageNumber="33">holotype</typeStatus>
slide (registration number: PXM-20120515) and
<specimenCount id="9D16C095FFB2FFF6FE1B3DC6FD631B96" box="[498,650,1669,1695]" pageId="2" pageNumber="33" type="generic" typeStatus="paratype">one paratype</specimenCount>
slide (registration number:
<collectionCode id="ED0193D9FFB2FFF6FEBD3DEBFE2A1BCB" box="[340,451,1704,1730]" country="United Kingdom" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34665" lsid="urn:lsid:biocol.org:col:34665" name="Natural History Museum, London" pageId="2" pageNumber="33" type="Museum">NHMUK</collectionCode>
2013.8.15.1) with protargol stained-specimens are deposited in the
<location id="8ECF5DC7FFB2FFF6FD8D3D89FECA1A0F" LSID="urn:lsid:plazi:treatment:03B9BA0AFFB1FFF1FCC03C45FAB31C14:8ECF5DC7FFB2FFF6FD8D3D89FECA1A0F" country="China" county="London" municipality="Natural History Museum" name="Laboratory of Protozoology" pageId="2" pageNumber="33">Laboratory of Protozoology</location>
,
<location id="8ECF5DC7FFB2FFF6FEDA3DAEFDEC1A0F" LSID="urn:lsid:plazi:treatment:03B9BA0AFFB1FFF1FCC03C45FAB31C14:8ECF5DC7FFB2FFF6FEDA3DAEFDEC1A0F" box="[307,517,1772,1799]" country="China" county="London" municipality="Natural History Museum" name="Ocean University" pageId="2" pageNumber="33">Ocean University</location>
of
<collectingCountry id="F3074B8CFFB2FFF6FDDA3DAFFD901A0F" box="[563,633,1772,1798]" name="China" pageId="2" pageNumber="33">China</collectingCountry>
(
<collectionCode id="ED0193D9FFB2FFF6FD653DAEFD241A0E" box="[652,717,1773,1799]" pageId="2" pageNumber="33">OUC</collectionCode>
) and the
<collectingMunicipality id="6BCB9166FFB2FFF6FF463C4CFE311A23" box="[175,472,1807,1834]" pageId="2" pageNumber="33">Natural History Museum</collectingMunicipality>
,
<collectingCounty id="62CE7390FFB2FFF6FE0A3C4CFDA81A20" box="[483,577,1807,1833]" pageId="2" pageNumber="33">London</collectingCounty>
, respectively
</materialsCitation>
.
</paragraph>
</subSubSection>
<subSubSection id="C30A5897FFB2FFF6FF433C71FE591A67" pageId="2" pageNumber="33" type="etymology">
<paragraph id="8BAF0B1CFFB2FFF6FF433C71FE591A67" blockId="2.[132,779,1391,1903]" pageId="2" pageNumber="33">
<emphasis id="B964D70EFFB2FFF6FF433C71FED21A45" bold="true" box="[170,315,1842,1868]" pageId="2" pageNumber="33">Etymology:</emphasis>
This new form named
<taxonomicName id="4C10709FFFB2FFF6FDB73C71FD511A45" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[606,696,1842,1868]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="2" pageNumber="33" phylum="Ciliophora" rank="species" species="elegans">
<emphasis id="B964D70EFFB2FFF6FDB73C71FD511A45" box="[606,696,1842,1868]" italics="true" pageId="2" pageNumber="33">elegans</emphasis>
</taxonomicName>
refers to its elegant body shape.
</paragraph>
</subSubSection>
<subSubSection id="C30A5897FFB2FFF1FCBF3E2CFEA219B8" lastPageId="5" lastPageNumber="36" pageId="2" pageNumber="33" type="description">
<paragraph id="8BAF0B1CFFB2FFF1FCBF3E2CFE661C49" blockId="2.[816,1465,1391,1938]" lastBlockId="5.[123,772,223,1940]" lastPageId="5" lastPageNumber="36" pageId="2" pageNumber="33">
<emphasis id="B964D70EFFB2FFF6FCBF3E2CFC181880" bold="true" box="[854,1009,1391,1417]" pageId="2" pageNumber="33">Description:</emphasis>
The body is about 100 × 50 µm
<emphasis id="B964D70EFFB2FFF6FA493E33FC8818A5" italics="true" pageId="2" pageNumber="33">in vivo</emphasis>
, slender oval in outline, with a distinctly pointed posterior end (
<figureCitation id="132B1799FFB2FFF6FC0C3EF6FB7A18C6" box="[997,1171,1461,1487]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="2" pageNumber="33">Figs 2A, H, I</figureCitation>
). Buccal field cavity is about 70% of body length with a conspicuous, saillike, undulating membrane (
<figureCitation id="132B1799FFB2FFF6FB633EB9FB081B1D" box="[1162,1249,1530,1556]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="2" pageNumber="33">Fig. 2I</figureCitation>
). Pellicle is rigid and slightly notched with closely arranged extrusomes, which is about 3 µm long (
<figureCitation id="132B1799FFB2FFF6FB9B3D7CFB391B50" box="[1138,1232,1599,1625]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="2" pageNumber="33">Fig. 2B</figureCitation>
). Cytoplasm is colourless to slightly grayish, packed with large amounts of green ingested algae and shining globules of varying size, food vacuoles which are usually large and filled with bacteria, and blue irregularly-shaped crystals (&lt;6 μm in diameter) (
<figureCitation id="132B1799FFB2FFF6FBD93DAEFB251A0E" box="[1072,1228,1773,1799]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="2" pageNumber="33">Figs 2A, H, I</figureCitation>
). One spherical ma- cronucleus, about 32 × 32 µm, located in anterior half of cell. No micronucleus is observed (
<figureCitation id="132B1799FFB2FFF6FB123C71FABE1A45" box="[1275,1367,1842,1868]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="2" pageNumber="33">Fig. 2P</figureCitation>
). Single contractile vacuole is about 10 µm in diameter, located slightly dorsally near posterior end of cell (
<figureCitation id="132B1799FFB2FFF6FAAE3C34FA431A98" box="[1351,1450,1911,1937]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="2" pageNumber="33">Fig. 2A</figureCitation>
). Somatic cilia is about 12 µm long (
<figureCitation id="132B1799FFB5FFF1FDCA3B9CFD401DF3" box="[547,681,223,250]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Figs 2A, L</figureCitation>
). There are about ten prolonged caudal cilia, each is about 30 µm in length (
<figureCitation id="132B1799FFB5FFF1FECA3A65FE6B1C49" box="[291,386,294,320]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Fig. 2K</figureCitation>
).
</paragraph>
<caption id="DF6F5B94FFB4FFF0FF6D3B9CFD8E1C40" ID-Table-UUID="DF6F5B94FFB4FFF0FF6D3B9CFD8E1C40" httpUri="http://table.plazi.org/id/DF6F5B94FFB4FFF0FF6D3B9CFD8E1C40" pageId="4" pageNumber="35" startId="4.[132,188,223,244]" targetBox="[132,1397,362,1430]" targetIsTable="true" targetPageId="4" targetType="table">
<paragraph id="8BAF0B1CFFB4FFF0FF6D3B9CFD8E1C40" blockId="4.[132,1463,223,329]" pageId="4" pageNumber="35">
<emphasis id="B964D70EFFB4FFF0FF6D3B9CFF3F1DFD" bold="true" box="[132,214,223,244]" pageId="4" pageNumber="35">Table 1.</emphasis>
Morphometric characterization of
<taxonomicName id="4C10709FFFB4FFF0FDC23B9CFD191DFD" authority="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao, 2015" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[555,752,223,244]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="4" pageNumber="35" phylum="Ciliophora" rank="species" species="elegans" status="spec. nov.">
<emphasis id="B964D70EFFB4FFF0FDC23B9CFD191DFD" box="[555,752,223,244]" italics="true" pageId="4" pageNumber="35">Pleuronema elegans</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A2576A75FFB4FFF0FD1E3BA3FCB11DFC" box="[759,856,224,245]" pageId="4" pageNumber="35" rank="species">spec. nov.</taxonomicNameLabel>
(upper row),
<taxonomicName id="4C10709FFFB4FFF0FC363BA3FB0B1DFD" authority="Calkins, 1902" authorityName="Calkins" authorityYear="1902" box="[991,1250,223,245]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="4" pageNumber="35" phylum="Ciliophora" rank="species" species="setigerum">
<emphasis id="B964D70EFFB4FFF0FC363BA3FBBF1DFC" box="[991,1110,224,245]" italics="true" pageId="4" pageNumber="35">P. setigerum</emphasis>
Calkins, 1902
</taxonomicName>
(second row),
<taxonomicName id="4C10709FFFB4FFF0FA9E3BA3FE8B1C19" authority="Wang et al., 2008" authorityName="Wang" authorityYear="2008" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="4" pageNumber="35" phylum="Ciliophora" rank="species" species="grolierei">
<emphasis id="B964D70EFFB4FFF0FA9E3BA3FF5F1C19" italics="true" pageId="4" pageNumber="35">P. grolierei</emphasis>
Wang
<emphasis id="B964D70EFFB4FFF0FF1F3BBFFEC91C19" box="[246,288,251,273]" italics="true" pageId="4" pageNumber="35">et al</emphasis>
., 2008
</taxonomicName>
(third row) and
<taxonomicName id="4C10709FFFB4FFF0FE123BBFFD5F1C19" authority="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao, 2015" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[507,694,251,273]" class="Oligohymenophorea" family="Uronematidae" genus="Uronema" kingdom="Chromista" order="Hymenostomatida" pageId="4" pageNumber="35" phylum="Ciliophora" rank="species" species="orientalis" status="spec. nov.">
<emphasis id="B964D70EFFB4FFF0FE123BBFFD5F1C19" box="[507,694,251,273]" italics="true" pageId="4" pageNumber="35">Uronema orientalis</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A2576A75FFB4FFF0FD523BBFFCF31C18" box="[699,794,252,273]" pageId="4" pageNumber="35" rank="species">spec. nov.</taxonomicNameLabel>
(lower row). Data based on protargol stained specimens. All measurements in µm. Abbreviations: CV coefficient of variation in %; Max maximum; Mean arithmetic mean; Min minimum; n number of individuals examined; SD standard deviation.
</paragraph>
</caption>
<paragraph id="8BAF0B1CFFB4FFF0FF6D3A29FA9C189F" pageId="4" pageNumber="35">
<table id="F910F9BCFFB4000BFF6D3A29FA9C189F" box="[132,1397,362,1430]" gridcols="7" gridrows="29" pageId="4" pageNumber="35">
<tr id="3520095EFFB4000BFF6D3A29FA9C1C74" box="[132,1397,362,381]" gridrow="0" pageId="4" pageNumber="35">
<th id="76F16022FFB4000BFF6D3A29FE6A1C74" box="[132,387,362,381]" gridcol="0" gridrow="0" pageId="4" pageNumber="35">Character</th>
<th id="76F16022FFB4000BFDB33A29FD681C74" box="[602,641,362,381]" gridcol="1" gridrow="0" pageId="4" pageNumber="35">Min</th>
<th id="76F16022FFB4000BFD1A3A29FCF21C74" box="[755,795,362,381]" gridcol="2" gridrow="0" pageId="4" pageNumber="35">Max</th>
<th id="76F16022FFB4000BFC943A29FC501C74" box="[893,953,362,381]" gridcol="3" gridrow="0" pageId="4" pageNumber="35">Mean</th>
<th id="76F16022FFB4000BFBC33A29FBAF1C74" box="[1066,1094,362,381]" gridcol="4" gridrow="0" pageId="4" pageNumber="35">SD</th>
<th id="76F16022FFB4000BFB2A3A29FB071C74" box="[1219,1262,362,381]" gridcol="5" gridrow="0" pageId="4" pageNumber="35">CV</th>
<th id="76F16022FFB4000BFA893A29FA9C1C74" box="[1376,1397,362,381]" gridcol="6" gridrow="0" pageId="4" pageNumber="35">n</th>
</tr>
<tr id="3520095EFFB4000BFF6D3ACCFA9C1CAB" box="[132,1397,399,418]" gridrow="1" pageId="4" pageNumber="35">
<th id="76F16022FFB4000BFF6D3ACCFE6A1CAB" box="[132,387,399,418]" gridcol="0" gridrow="1" pageId="4" pageNumber="35">Body length</th>
<td id="76F16022FFB4000BFDB33ACCFD681CAB" box="[602,641,399,418]" gridcol="1" gridrow="1" pageId="4" pageNumber="35">104</td>
<td id="76F16022FFB4000BFD1A3ACCFCF21CAB" box="[755,795,399,418]" gridcol="2" gridrow="1" pageId="4" pageNumber="35">122</td>
<td id="76F16022FFB4000BFC943ACCFC501CAB" box="[893,953,399,418]" gridcol="3" gridrow="1" pageId="4" pageNumber="35">111.7</td>
<td id="76F16022FFB4000BFBC33ACCFBAF1CAB" box="[1066,1094,399,418]" gridcol="4" gridrow="1" pageId="4" pageNumber="35">4.8</td>
<td id="76F16022FFB4000BFB2A3ACCFB071CAB" box="[1219,1262,399,418]" gridcol="5" gridrow="1" pageId="4" pageNumber="35">4.3</td>
<td id="76F16022FFB4000BFA893ACCFA9C1CAB" box="[1376,1397,399,418]" gridcol="6" gridrow="1" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3AF6FA9C1CC1" box="[132,1397,437,456]" gridrow="2" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33AF6FD681CC1" box="[602,641,437,456]" gridcol="1" gridrow="2" pageId="4" pageNumber="35">42</td>
<td id="76F16022FFB4000BFD1A3AF6FCF21CC1" box="[755,795,437,456]" gridcol="2" gridrow="2" pageId="4" pageNumber="35">58</td>
<td id="76F16022FFB4000BFC943AF6FC501CC1" box="[893,953,437,456]" gridcol="3" gridrow="2" pageId="4" pageNumber="35">50.2</td>
<td id="76F16022FFB4000BFBC33AF6FBAF1CC1" box="[1066,1094,437,456]" gridcol="4" gridrow="2" pageId="4" pageNumber="35">5.1</td>
<td id="76F16022FFB4000BFB2A3AF6FB071CC1" box="[1219,1262,437,456]" gridcol="5" gridrow="2" pageId="4" pageNumber="35">10.2</td>
<td id="76F16022FFB4000BFA893AF6FA9C1CC1" box="[1376,1397,437,456]" gridcol="6" gridrow="2" pageId="4" pageNumber="35">19</td>
</tr>
<tr id="3520095EFFB4000BFF6D3A99FA9C1CE4" box="[132,1397,474,493]" gridrow="3" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33A99FD681CE4" box="[602,641,474,493]" gridcol="1" gridrow="3" pageId="4" pageNumber="35">32</td>
<td id="76F16022FFB4000BFD1A3A99FCF21CE4" box="[755,795,474,493]" gridcol="2" gridrow="3" pageId="4" pageNumber="35">45</td>
<td id="76F16022FFB4000BFC943A99FC501CE4" box="[893,953,474,493]" gridcol="3" gridrow="3" pageId="4" pageNumber="35">38.4</td>
<td id="76F16022FFB4000BFBC33A99FBAF1CE4" box="[1066,1094,474,493]" gridcol="4" gridrow="3" pageId="4" pageNumber="35">7.1</td>
<td id="76F16022FFB4000BFB2A3A99FB071CE4" box="[1219,1262,474,493]" gridcol="5" gridrow="3" pageId="4" pageNumber="35">3.7</td>
<td id="76F16022FFB4000BFA893A99FA9C1CE4" box="[1376,1397,474,493]" gridcol="6" gridrow="3" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3ABCFA9C1F1A" box="[132,1397,511,531]" gridrow="4" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33ABCFD681F1A" box="[602,641,511,531]" gridcol="1" gridrow="4" pageId="4" pageNumber="35">74</td>
<td id="76F16022FFB4000BFD1A3ABCFCF21F1A" box="[755,795,511,531]" gridcol="2" gridrow="4" pageId="4" pageNumber="35">82</td>
<td id="76F16022FFB4000BFC943ABCFC501F1A" box="[893,953,511,531]" gridcol="3" gridrow="4" pageId="4" pageNumber="35">80.3</td>
<td id="76F16022FFB4000BFBC33ABCFBAF1F1A" box="[1066,1094,511,531]" gridcol="4" gridrow="4" pageId="4" pageNumber="35">5.3</td>
<td id="76F16022FFB4000BFB2A3ABCFB071F1A" box="[1219,1262,511,531]" gridcol="5" gridrow="4" pageId="4" pageNumber="35">6.2</td>
<td id="76F16022FFB4000BFA893ABCFA9C1F1A" box="[1376,1397,511,531]" gridcol="6" gridrow="4" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3966FA9C1F31" box="[132,1397,549,568]" gridrow="5" pageId="4" pageNumber="35">
<th id="76F16022FFB4000BFF6D3966FE6A1F31" box="[132,387,549,568]" gridcol="0" gridrow="5" pageId="4" pageNumber="35">Body width</th>
<td id="76F16022FFB4000BFDB33966FD681F31" box="[602,641,549,568]" gridcol="1" gridrow="5" pageId="4" pageNumber="35">58</td>
<td id="76F16022FFB4000BFD1A3966FCF21F31" box="[755,795,549,568]" gridcol="2" gridrow="5" pageId="4" pageNumber="35">66</td>
<td id="76F16022FFB4000BFC943966FC501F31" box="[893,953,549,568]" gridcol="3" gridrow="5" pageId="4" pageNumber="35">62.4</td>
<td id="76F16022FFB4000BFBC33966FBAF1F31" box="[1066,1094,549,568]" gridcol="4" gridrow="5" pageId="4" pageNumber="35">2.7</td>
<td id="76F16022FFB4000BFB2A3966FB071F31" box="[1219,1262,549,568]" gridcol="5" gridrow="5" pageId="4" pageNumber="35">4.3</td>
<td id="76F16022FFB4000BFA893966FA9C1F31" box="[1376,1397,549,568]" gridcol="6" gridrow="5" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3909FA9C1F57" box="[132,1397,586,606]" gridrow="6" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33909FD681F57" box="[602,641,586,606]" gridcol="1" gridrow="6" pageId="4" pageNumber="35">26</td>
<td id="76F16022FFB4000BFD1A3909FCF21F57" box="[755,795,586,606]" gridcol="2" gridrow="6" pageId="4" pageNumber="35">35</td>
<td id="76F16022FFB4000BFC943909FC501F57" box="[893,953,586,606]" gridcol="3" gridrow="6" pageId="4" pageNumber="35">30.4</td>
<td id="76F16022FFB4000BFBC33909FBAF1F57" box="[1066,1094,586,606]" gridcol="4" gridrow="6" pageId="4" pageNumber="35">2.9</td>
<td id="76F16022FFB4000BFB2A3909FB071F57" box="[1219,1262,586,606]" gridcol="5" gridrow="6" pageId="4" pageNumber="35">9.5</td>
<td id="76F16022FFB4000BFA893909FA9C1F57" box="[1376,1397,586,606]" gridcol="6" gridrow="6" pageId="4" pageNumber="35">19</td>
</tr>
<tr id="3520095EFFB4000BFF6D3933FA9C1F8A" box="[132,1397,624,643]" gridrow="7" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33933FD681F8A" box="[602,641,624,643]" gridcol="1" gridrow="7" pageId="4" pageNumber="35">18</td>
<td id="76F16022FFB4000BFD1A3933FCF21F8A" box="[755,795,624,643]" gridcol="2" gridrow="7" pageId="4" pageNumber="35">24</td>
<td id="76F16022FFB4000BFC943933FC501F8A" box="[893,953,624,643]" gridcol="3" gridrow="7" pageId="4" pageNumber="35">21.1</td>
<td id="76F16022FFB4000BFBC33933FBAF1F8A" box="[1066,1094,624,643]" gridcol="4" gridrow="7" pageId="4" pageNumber="35">4.3</td>
<td id="76F16022FFB4000BFB2A3933FB071F8A" box="[1219,1262,624,643]" gridcol="5" gridrow="7" pageId="4" pageNumber="35">4.6</td>
<td id="76F16022FFB4000BFA893933FA9C1F8A" box="[1376,1397,624,643]" gridcol="6" gridrow="7" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D39D6FA9C1FA0" box="[132,1397,661,681]" gridrow="8" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB339D6FD681FA0" box="[602,641,661,681]" gridcol="1" gridrow="8" pageId="4" pageNumber="35">28</td>
<td id="76F16022FFB4000BFD1A39D6FCF21FA0" box="[755,795,661,681]" gridcol="2" gridrow="8" pageId="4" pageNumber="35">39</td>
<td id="76F16022FFB4000BFC9439D6FC501FA0" box="[893,953,661,681]" gridcol="3" gridrow="8" pageId="4" pageNumber="35">34.2</td>
<td id="76F16022FFB4000BFBC339D6FBAF1FA0" box="[1066,1094,661,681]" gridcol="4" gridrow="8" pageId="4" pageNumber="35">6.1</td>
<td id="76F16022FFB4000BFB2A39D6FB071FA0" box="[1219,1262,661,681]" gridcol="5" gridrow="8" pageId="4" pageNumber="35">5.6</td>
<td id="76F16022FFB4000BFA8939D6FA9C1FA0" box="[1376,1397,661,681]" gridcol="6" gridrow="8" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D39F8FA9C1FC7" box="[132,1397,699,718]" gridrow="9" pageId="4" pageNumber="35">
<th id="76F16022FFB4000BFF6D39F8FE6A1FC7" box="[132,387,699,718]" gridcol="0" gridrow="9" pageId="4" pageNumber="35">Number of somatic kineties</th>
<td id="76F16022FFB4000BFDB339F8FD681FC7" box="[602,641,699,718]" gridcol="1" gridrow="9" pageId="4" pageNumber="35">18</td>
<td id="76F16022FFB4000BFD1A39F8FCF21FC7" box="[755,795,699,718]" gridcol="2" gridrow="9" pageId="4" pageNumber="35">19</td>
<td id="76F16022FFB4000BFC9439F8FC501FC7" box="[893,953,699,718]" gridcol="3" gridrow="9" pageId="4" pageNumber="35">18.2</td>
<td id="76F16022FFB4000BFBC339F8FBAF1FC7" box="[1066,1094,699,718]" gridcol="4" gridrow="9" pageId="4" pageNumber="35">0.4</td>
<td id="76F16022FFB4000BFB2A39F8FB071FC7" box="[1219,1262,699,718]" gridcol="5" gridrow="9" pageId="4" pageNumber="35">2.2</td>
<td id="76F16022FFB4000BFA8939F8FA9C1FC7" box="[1376,1397,699,718]" gridcol="6" gridrow="9" pageId="4" pageNumber="35">20</td>
</tr>
<tr id="3520095EFFB4000BFF6D39A2FA9C1FFD" box="[132,1397,737,756]" gridrow="10" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB339A2FD681FFD" box="[602,641,737,756]" gridcol="1" gridrow="10" pageId="4" pageNumber="35">20</td>
<td id="76F16022FFB4000BFD1A39A2FCF21FFD" box="[755,795,737,756]" gridcol="2" gridrow="10" pageId="4" pageNumber="35">20</td>
<td id="76F16022FFB4000BFC9439A2FC501FFD" box="[893,953,737,756]" gridcol="3" gridrow="10" pageId="4" pageNumber="35">20.0</td>
<td id="76F16022FFB4000BFBC339A2FBAF1FFD" box="[1066,1094,737,756]" gridcol="4" gridrow="10" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFB2A39A2FB071FFD" box="[1219,1262,737,756]" gridcol="5" gridrow="10" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFA8939A2FA9C1FFD" box="[1376,1397,737,756]" gridcol="6" gridrow="10" pageId="4" pageNumber="35">18</td>
</tr>
<tr id="3520095EFFB4000BFF6D3845FA9C1E10" box="[132,1397,774,793]" gridrow="11" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33845FD681E10" box="[602,641,774,793]" gridcol="1" gridrow="11" pageId="4" pageNumber="35">12</td>
<td id="76F16022FFB4000BFD1A3845FCF21E10" box="[755,795,774,793]" gridcol="2" gridrow="11" pageId="4" pageNumber="35">14</td>
<td id="76F16022FFB4000BFC943845FC501E10" box="[893,953,774,793]" gridcol="3" gridrow="11" pageId="4" pageNumber="35">12.8</td>
<td id="76F16022FFB4000BFBC33845FBAF1E10" box="[1066,1094,774,793]" gridcol="4" gridrow="11" pageId="4" pageNumber="35">3.2</td>
<td id="76F16022FFB4000BFB2A3845FB071E10" box="[1219,1262,774,793]" gridcol="5" gridrow="11" pageId="4" pageNumber="35">2.7</td>
<td id="76F16022FFB4000BFA893845FA9C1E10" box="[1376,1397,774,793]" gridcol="6" gridrow="11" pageId="4" pageNumber="35">18</td>
</tr>
<tr id="3520095EFFB4000BFF6D3868FA9C1E37" box="[132,1397,811,830]" gridrow="12" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33868FD681E37" box="[602,641,811,830]" gridcol="1" gridrow="12" pageId="4" pageNumber="35">18</td>
<td id="76F16022FFB4000BFD1A3868FCF21E37" box="[755,795,811,830]" gridcol="2" gridrow="12" pageId="4" pageNumber="35">22</td>
<td id="76F16022FFB4000BFC943868FC501E37" box="[893,953,811,830]" gridcol="3" gridrow="12" pageId="4" pageNumber="35">20.2</td>
<td id="76F16022FFB4000BFBC33868FBAF1E37" box="[1066,1094,811,830]" gridcol="4" gridrow="12" pageId="4" pageNumber="35">6.2</td>
<td id="76F16022FFB4000BFB2A3868FB071E37" box="[1219,1262,811,830]" gridcol="5" gridrow="12" pageId="4" pageNumber="35">2.6</td>
<td id="76F16022FFB4000BFA893868FA9C1E37" box="[1376,1397,811,830]" gridcol="6" gridrow="12" pageId="4" pageNumber="35">19</td>
</tr>
<tr id="3520095EFFB4000BFF6D3812FA9C1E6D" box="[132,1397,849,868]" gridrow="13" pageId="4" pageNumber="35">
<th id="76F16022FFB4000BFF6D3812FE6A1E6D" box="[132,387,849,868]" gridcol="0" gridrow="13" pageId="4" pageNumber="35">Number of preoral kineties</th>
<td id="76F16022FFB4000BFDB33812FD681E6D" box="[602,641,849,868]" gridcol="1" gridrow="13" pageId="4" pageNumber="35">2</td>
<td id="76F16022FFB4000BFD1A3812FCF21E6D" box="[755,795,849,868]" gridcol="2" gridrow="13" pageId="4" pageNumber="35">2</td>
<td id="76F16022FFB4000BFC943812FC501E6D" box="[893,953,849,868]" gridcol="3" gridrow="13" pageId="4" pageNumber="35">2.0</td>
<td id="76F16022FFB4000BFBC33812FBAF1E6D" box="[1066,1094,849,868]" gridcol="4" gridrow="13" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFB2A3812FB071E6D" box="[1219,1262,849,868]" gridcol="5" gridrow="13" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFA893812FA9C1E6D" box="[1376,1397,849,868]" gridcol="6" gridrow="13" pageId="4" pageNumber="35">15</td>
</tr>
<tr id="3520095EFFB4000BFF6D3834FA9C1E83" box="[132,1397,887,906]" gridrow="14" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33834FD681E83" box="[602,641,887,906]" gridcol="1" gridrow="14" pageId="4" pageNumber="35"></td>
<td id="76F16022FFB4000BFD1A3834FCF21E83" box="[755,795,887,906]" gridcol="2" gridrow="14" pageId="4" pageNumber="35"></td>
<td id="76F16022FFB4000BFC943834FC501E83" box="[893,953,887,906]" gridcol="3" gridrow="14" pageId="4" pageNumber="35"></td>
<td id="76F16022FFB4000BFBC33834FBAF1E83" box="[1066,1094,887,906]" gridcol="4" gridrow="14" pageId="4" pageNumber="35"></td>
<td id="76F16022FFB4000BFB2A3834FB071E83" box="[1219,1262,887,906]" gridcol="5" gridrow="14" pageId="4" pageNumber="35"></td>
<td id="76F16022FFB4000BFA893834FA9C1E83" box="[1376,1397,887,906]" gridcol="6" gridrow="14" pageId="4" pageNumber="35"></td>
</tr>
<tr id="3520095EFFB4000BFF6D38DFFA9C1EA6" box="[132,1397,924,943]" gridrow="15" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB338DFFD681EA6" box="[602,641,924,943]" gridcol="1" gridrow="15" pageId="4" pageNumber="35">5</td>
<td id="76F16022FFB4000BFD1A38DFFCF21EA6" box="[755,795,924,943]" gridcol="2" gridrow="15" pageId="4" pageNumber="35">6</td>
<td id="76F16022FFB4000BFC9438DFFC501EA6" box="[893,953,924,943]" gridcol="3" gridrow="15" pageId="4" pageNumber="35">5.2</td>
<td id="76F16022FFB4000BFBC338DFFBAF1EA6" box="[1066,1094,924,943]" gridcol="4" gridrow="15" pageId="4" pageNumber="35">3.6</td>
<td id="76F16022FFB4000BFB2A38DFFB071EA6" box="[1219,1262,924,943]" gridcol="5" gridrow="15" pageId="4" pageNumber="35">2.7</td>
<td id="76F16022FFB4000BFA8938DFFA9C1EA6" box="[1376,1397,924,943]" gridcol="6" gridrow="15" pageId="4" pageNumber="35">16</td>
</tr>
<tr id="3520095EFFB4000BFF6D3882FA9C1EDD" box="[132,1397,961,980]" gridrow="16" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33882FD681EDD" box="[602,641,961,980]" gridcol="1" gridrow="16" pageId="4" pageNumber="35">1</td>
<td id="76F16022FFB4000BFD1A3882FCF21EDD" box="[755,795,961,980]" gridcol="2" gridrow="16" pageId="4" pageNumber="35">1</td>
<td id="76F16022FFB4000BFC943882FC501EDD" box="[893,953,961,980]" gridcol="3" gridrow="16" pageId="4" pageNumber="35">1.0</td>
<td id="76F16022FFB4000BFBC33882FBAF1EDD" box="[1066,1094,961,980]" gridcol="4" gridrow="16" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFB2A3882FB071EDD" box="[1219,1262,961,980]" gridcol="5" gridrow="16" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFA893882FA9C1EDD" box="[1376,1397,961,980]" gridcol="6" gridrow="16" pageId="4" pageNumber="35">12</td>
</tr>
<tr id="3520095EFFB4000BFF6D38A4FA9C1EF3" box="[132,1397,999,1018]" gridrow="17" pageId="4" pageNumber="35">
<th id="76F16022FFB4000BFF6D38A4FE6A1EF3" box="[132,387,999,1018]" gridcol="0" gridrow="17" pageId="4" pageNumber="35">Number of kinety rows in M3</th>
<td id="76F16022FFB4000BFDB338A4FD681EF3" box="[602,641,999,1018]" gridcol="1" gridrow="17" pageId="4" pageNumber="35">3</td>
<td id="76F16022FFB4000BFD1A38A4FCF21EF3" box="[755,795,999,1018]" gridcol="2" gridrow="17" pageId="4" pageNumber="35">3</td>
<td id="76F16022FFB4000BFC9438A4FC501EF3" box="[893,953,999,1018]" gridcol="3" gridrow="17" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFBC338A4FBAF1EF3" box="[1066,1094,999,1018]" gridcol="4" gridrow="17" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFB2A38A4FB071EF3" box="[1219,1262,999,1018]" gridcol="5" gridrow="17" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFA8938A4FA9C1EF3" box="[1376,1397,999,1018]" gridcol="6" gridrow="17" pageId="4" pageNumber="35">15</td>
</tr>
<tr id="3520095EFFB4000BFF6D3F4FFA9C1916" box="[132,1397,1036,1055]" gridrow="18" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33F4FFD681916" box="[602,641,1036,1055]" gridcol="1" gridrow="18" pageId="4" pageNumber="35">3</td>
<td id="76F16022FFB4000BFD1A3F4FFCF21916" box="[755,795,1036,1055]" gridcol="2" gridrow="18" pageId="4" pageNumber="35">3</td>
<td id="76F16022FFB4000BFC943F4FFC501916" box="[893,953,1036,1055]" gridcol="3" gridrow="18" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFBC33F4FFBAF1916" box="[1066,1094,1036,1055]" gridcol="4" gridrow="18" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFB2A3F4FFB071916" box="[1219,1262,1036,1055]" gridcol="5" gridrow="18" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFA893F4FFA9C1916" box="[1376,1397,1036,1055]" gridcol="6" gridrow="18" pageId="4" pageNumber="35">14</td>
</tr>
<tr id="3520095EFFB4000BFF6D3F71FA9C194C" box="[132,1397,1074,1093]" gridrow="19" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33F71FD68194C" box="[602,641,1074,1093]" gridcol="1" gridrow="19" pageId="4" pageNumber="35">3</td>
<td id="76F16022FFB4000BFD1A3F71FCF2194C" box="[755,795,1074,1093]" gridcol="2" gridrow="19" pageId="4" pageNumber="35">3</td>
<td id="76F16022FFB4000BFC943F71FC50194C" box="[893,953,1074,1093]" gridcol="3" gridrow="19" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFBC33F71FBAF194C" box="[1066,1094,1074,1093]" gridcol="4" gridrow="19" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFB2A3F71FB07194C" box="[1219,1262,1074,1093]" gridcol="5" gridrow="19" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFA893F71FA9C194C" box="[1376,1397,1074,1093]" gridcol="6" gridrow="19" pageId="4" pageNumber="35">14</td>
</tr>
<tr id="3520095EFFB4000BFF6D3F14FA9C1963" box="[132,1397,1111,1130]" gridrow="20" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33F14FD681963" box="[602,641,1111,1130]" gridcol="1" gridrow="20" pageId="4" pageNumber="35">2</td>
<td id="76F16022FFB4000BFD1A3F14FCF21963" box="[755,795,1111,1130]" gridcol="2" gridrow="20" pageId="4" pageNumber="35">2</td>
<td id="76F16022FFB4000BFC943F14FC501963" box="[893,953,1111,1130]" gridcol="3" gridrow="20" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFBC33F14FBAF1963" box="[1066,1094,1111,1130]" gridcol="4" gridrow="20" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFB2A3F14FB071963" box="[1219,1262,1111,1130]" gridcol="5" gridrow="20" pageId="4" pageNumber="35">0</td>
<td id="76F16022FFB4000BFA893F14FA9C1963" box="[1376,1397,1111,1130]" gridcol="6" gridrow="20" pageId="4" pageNumber="35">13</td>
</tr>
<tr id="3520095EFFB4000BFF6D3F3EFA9C1999" box="[132,1397,1149,1168]" gridrow="21" pageId="4" pageNumber="35">
<th id="76F16022FFB4000BFF6D3F3EFE6A1999" box="[132,387,1149,1168]" gridcol="0" gridrow="21" pageId="4" pageNumber="35">Length of macronucleus</th>
<td id="76F16022FFB4000BFDB33F3EFD681999" box="[602,641,1149,1168]" gridcol="1" gridrow="21" pageId="4" pageNumber="35">29</td>
<td id="76F16022FFB4000BFD1A3F3EFCF21999" box="[755,795,1149,1168]" gridcol="2" gridrow="21" pageId="4" pageNumber="35">36</td>
<td id="76F16022FFB4000BFC943F3EFC501999" box="[893,953,1149,1168]" gridcol="3" gridrow="21" pageId="4" pageNumber="35">32.4</td>
<td id="76F16022FFB4000BFBC33F3EFBAF1999" box="[1066,1094,1149,1168]" gridcol="4" gridrow="21" pageId="4" pageNumber="35">2</td>
<td id="76F16022FFB4000BFB2A3F3EFB071999" box="[1219,1262,1149,1168]" gridcol="5" gridrow="21" pageId="4" pageNumber="35">6.2</td>
<td id="76F16022FFB4000BFA893F3EFA9C1999" box="[1376,1397,1149,1168]" gridcol="6" gridrow="21" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3FE1FA9C19BC" box="[132,1397,1186,1205]" gridrow="22" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33FE1FD6819BC" box="[602,641,1186,1205]" gridcol="1" gridrow="22" pageId="4" pageNumber="35">16</td>
<td id="76F16022FFB4000BFD1A3FE1FCF219BC" box="[755,795,1186,1205]" gridcol="2" gridrow="22" pageId="4" pageNumber="35">19</td>
<td id="76F16022FFB4000BFC943FE1FC5019BC" box="[893,953,1186,1205]" gridcol="3" gridrow="22" pageId="4" pageNumber="35">16.5</td>
<td id="76F16022FFB4000BFBC33FE1FBAF19BC" box="[1066,1094,1186,1205]" gridcol="4" gridrow="22" pageId="4" pageNumber="35">0.9</td>
<td id="76F16022FFB4000BFB2A3FE1FB0719BC" box="[1219,1262,1186,1205]" gridcol="5" gridrow="22" pageId="4" pageNumber="35">5.5</td>
<td id="76F16022FFB4000BFA893FE1FA9C19BC" box="[1376,1397,1186,1205]" gridcol="6" gridrow="22" pageId="4" pageNumber="35">19</td>
</tr>
<tr id="3520095EFFB4000BFF6D3F8BFA9C19D2" box="[132,1397,1224,1243]" gridrow="23" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33F8BFD6819D2" box="[602,641,1224,1243]" gridcol="1" gridrow="23" pageId="4" pageNumber="35">9</td>
<td id="76F16022FFB4000BFD1A3F8BFCF219D2" box="[755,795,1224,1243]" gridcol="2" gridrow="23" pageId="4" pageNumber="35">12</td>
<td id="76F16022FFB4000BFC943F8BFC5019D2" box="[893,953,1224,1243]" gridcol="3" gridrow="23" pageId="4" pageNumber="35">11.2</td>
<td id="76F16022FFB4000BFBC33F8BFBAF19D2" box="[1066,1094,1224,1243]" gridcol="4" gridrow="23" pageId="4" pageNumber="35">0.8</td>
<td id="76F16022FFB4000BFB2A3F8BFB0719D2" box="[1219,1262,1224,1243]" gridcol="5" gridrow="23" pageId="4" pageNumber="35">4.9</td>
<td id="76F16022FFB4000BFA893F8BFA9C19D2" box="[1376,1397,1224,1243]" gridcol="6" gridrow="23" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3FAEFA9C1809" box="[132,1397,1261,1280]" gridrow="24" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33FAEFD681809" box="[602,641,1261,1280]" gridcol="1" gridrow="24" pageId="4" pageNumber="35">14</td>
<td id="76F16022FFB4000BFD1A3FAEFCF21809" box="[755,795,1261,1280]" gridcol="2" gridrow="24" pageId="4" pageNumber="35">17</td>
<td id="76F16022FFB4000BFC943FAEFC501809" box="[893,953,1261,1280]" gridcol="3" gridrow="24" pageId="4" pageNumber="35">14.8</td>
<td id="76F16022FFB4000BFBC33FAEFBAF1809" box="[1066,1094,1261,1280]" gridcol="4" gridrow="24" pageId="4" pageNumber="35">7.1</td>
<td id="76F16022FFB4000BFB2A3FAEFB071809" box="[1219,1262,1261,1280]" gridcol="5" gridrow="24" pageId="4" pageNumber="35">6.2</td>
<td id="76F16022FFB4000BFA893FAEFA9C1809" box="[1376,1397,1261,1280]" gridcol="6" gridrow="24" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3E51FA9C182F" box="[132,1397,1298,1318]" gridrow="25" pageId="4" pageNumber="35">
<th id="76F16022FFB4000BFF6D3E51FE6A182F" box="[132,387,1298,1318]" gridcol="0" gridrow="25" pageId="4" pageNumber="35">Width of macronucleus</th>
<td id="76F16022FFB4000BFDB33E51FD68182F" box="[602,641,1298,1318]" gridcol="1" gridrow="25" pageId="4" pageNumber="35">28</td>
<td id="76F16022FFB4000BFD1A3E51FCF2182F" box="[755,795,1298,1318]" gridcol="2" gridrow="25" pageId="4" pageNumber="35">32</td>
<td id="76F16022FFB4000BFC943E51FC50182F" box="[893,953,1298,1318]" gridcol="3" gridrow="25" pageId="4" pageNumber="35">31</td>
<td id="76F16022FFB4000BFBC33E51FBAF182F" box="[1066,1094,1298,1318]" gridcol="4" gridrow="25" pageId="4" pageNumber="35">1.6</td>
<td id="76F16022FFB4000BFB2A3E51FB07182F" box="[1219,1262,1298,1318]" gridcol="5" gridrow="25" pageId="4" pageNumber="35">5.2</td>
<td id="76F16022FFB4000BFA893E51FA9C182F" box="[1376,1397,1298,1318]" gridcol="6" gridrow="25" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3E7BFA9C1842" box="[132,1397,1336,1355]" gridrow="26" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33E7BFD681842" box="[602,641,1336,1355]" gridcol="1" gridrow="26" pageId="4" pageNumber="35">14</td>
<td id="76F16022FFB4000BFD1A3E7BFCF21842" box="[755,795,1336,1355]" gridcol="2" gridrow="26" pageId="4" pageNumber="35">16</td>
<td id="76F16022FFB4000BFC943E7BFC501842" box="[893,953,1336,1355]" gridcol="3" gridrow="26" pageId="4" pageNumber="35">14.7</td>
<td id="76F16022FFB4000BFBC33E7BFBAF1842" box="[1066,1094,1336,1355]" gridcol="4" gridrow="26" pageId="4" pageNumber="35">0.7</td>
<td id="76F16022FFB4000BFB2A3E7BFB071842" box="[1219,1262,1336,1355]" gridcol="5" gridrow="26" pageId="4" pageNumber="35">4.8</td>
<td id="76F16022FFB4000BFA893E7BFA9C1842" box="[1376,1397,1336,1355]" gridcol="6" gridrow="26" pageId="4" pageNumber="35">19</td>
</tr>
<tr id="3520095EFFB4000BFF6D3E1EFA9C1879" box="[132,1397,1373,1392]" gridrow="27" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33E1EFD681879" box="[602,641,1373,1392]" gridcol="1" gridrow="27" pageId="4" pageNumber="35">10</td>
<td id="76F16022FFB4000BFD1A3E1EFCF21879" box="[755,795,1373,1392]" gridcol="2" gridrow="27" pageId="4" pageNumber="35">13</td>
<td id="76F16022FFB4000BFC943E1EFC501879" box="[893,953,1373,1392]" gridcol="3" gridrow="27" pageId="4" pageNumber="35">8.5</td>
<td id="76F16022FFB4000BFBC33E1EFBAF1879" box="[1066,1094,1373,1392]" gridcol="4" gridrow="27" pageId="4" pageNumber="35">3.1</td>
<td id="76F16022FFB4000BFB2A3E1EFB071879" box="[1219,1262,1373,1392]" gridcol="5" gridrow="27" pageId="4" pageNumber="35">5.7</td>
<td id="76F16022FFB4000BFA893E1EFA9C1879" box="[1376,1397,1373,1392]" gridcol="6" gridrow="27" pageId="4" pageNumber="35">21</td>
</tr>
<tr id="3520095EFFB4000BFF6D3EC0FA9C189F" box="[132,1397,1411,1430]" gridrow="28" pageId="4" pageNumber="35" rowspan-0="1">
<td id="76F16022FFB4000BFDB33EC0FD68189F" box="[602,641,1411,1430]" gridcol="1" gridrow="28" pageId="4" pageNumber="35">14</td>
<td id="76F16022FFB4000BFD1A3EC0FCF2189F" box="[755,795,1411,1430]" gridcol="2" gridrow="28" pageId="4" pageNumber="35">18</td>
<td id="76F16022FFB4000BFC943EC0FC50189F" box="[893,953,1411,1430]" gridcol="3" gridrow="28" pageId="4" pageNumber="35">15.9</td>
<td id="76F16022FFB4000BFBC33EC0FBAF189F" box="[1066,1094,1411,1430]" gridcol="4" gridrow="28" pageId="4" pageNumber="35">2.7</td>
<td id="76F16022FFB4000BFB2A3EC0FB07189F" box="[1219,1262,1411,1430]" gridcol="5" gridrow="28" pageId="4" pageNumber="35">4.8</td>
<td id="76F16022FFB4000BFA893EC0FA9C189F" box="[1376,1397,1411,1430]" gridcol="6" gridrow="28" pageId="4" pageNumber="35">21</td>
</tr>
</table>
</paragraph>
<caption id="DF6F5B94FFB4FFF0FF6D3D02FD481A67" ID-DOI="http://doi.org/10.5281/zenodo.8377647" ID-Zenodo-Dep="8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="4" pageNumber="35" startId="4.[132,167,1601,1623]" targetBox="[113,1445,330,1889]" targetPageId="3" targetType="figure">
<paragraph id="8BAF0B1CFFB4FFF0FF6D3D02FD481A67" blockId="4.[131,1464,1601,1903]" pageId="4" pageNumber="35">
<emphasis id="B964D70EFFB4FFF0FF6D3D02FF2C1B5F" bold="true" box="[132,197,1601,1623]" pageId="4" pageNumber="35">Fig. 2.</emphasis>
<taxonomicName id="4C10709FFFB4FFF0FF243D02FE781B5F" authority="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao, 2015" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[205,401,1601,1622]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="4" pageNumber="35" phylum="Ciliophora" rank="species" species="elegans" status="spec. nov.">
<emphasis id="B964D70EFFB4FFF0FF243D02FE781B5F" box="[205,401,1601,1622]" italics="true" pageId="4" pageNumber="35">Pleuronema elegans</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A2576A75FFB4FFF0FE7E3D02FE101B5F" box="[407,505,1601,1622]" pageId="4" pageNumber="35" rank="species">spec. nov.</taxonomicNameLabel>
<emphasis id="B964D70EFFB4FFF0FDE93D02FDA81B5F" box="[512,577,1601,1622]" italics="true" pageId="4" pageNumber="35">in vivo</emphasis>
(A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C).
<emphasis id="B964D70EFFB4FFF0FAC33D02FAB21B5F" bold="true" box="[1322,1371,1601,1622]" pageId="4" pageNumber="35">A, H</emphasis>
ventral view of a representative individual;
<emphasis id="B964D70EFFB4FFF0FE0A3D1EFE1A1B7B" bold="true" box="[483,499,1629,1650]" pageId="4" pageNumber="35">B</emphasis>
detailed view of cortex to demonstrate arrangement of extrusomes;
<emphasis id="B964D70EFFB4FFF0FB753D1EFB441B7A" bold="true" box="[1180,1197,1629,1651]" pageId="4" pageNumber="35">C</emphasis>
part of silverline system;
<emphasis id="B964D70EFFB4FFF0FF6C3D3AFF7F1B87" bold="true" box="[133,150,1657,1678]" pageId="4" pageNumber="35">D</emphasis>
swimming trace;
<emphasis id="B964D70EFFB4FFF0FEBE3D3AFE6B1B87" bold="true" box="[343,386,1657,1678]" pageId="4" pageNumber="35">E, F</emphasis>
ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus;
<emphasis id="B964D70EFFB4FFF0FA843D3AFA691B86" bold="true" box="[1389,1408,1657,1679]" pageId="4" pageNumber="35">G</emphasis>
detailed structure of the buccal area, arrow shows the straight posterior end of M2a;
<emphasis id="B964D70EFFB4FFF0FC583DD6FC531BA3" bold="true" box="[945,954,1685,1706]" pageId="4" pageNumber="35">I</emphasis>
ventral view, arrow marks paroral membrane and arrowhead shows ingested algae;
<emphasis id="B964D70EFFB4FFF0FE3B3DF2FE371BCF" bold="true" box="[466,478,1713,1734]" pageId="4" pageNumber="35">J</emphasis>
ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2;
<emphasis id="B964D70EFFB4FFF0FF6D3D8EFF7E1BEB" bold="true" box="[132,151,1741,1762]" pageId="4" pageNumber="35">K</emphasis>
ventral view, arrowheads point to caudal cilia;
<emphasis id="B964D70EFFB4FFF0FD6A3D8EFD7A1BEB" bold="true" box="[643,659,1741,1762]" pageId="4" pageNumber="35">L</emphasis>
ventral view, arrowheads mark somatic cilia;
<emphasis id="B964D70EFFB4FFF0FB863D8EFB6F1BEB" bold="true" box="[1135,1158,1741,1762]" pageId="4" pageNumber="35">M</emphasis>
infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies;
<emphasis id="B964D70EFFB4FFF0FCB13DAAFC801BF7" bold="true" box="[856,873,1769,1790]" pageId="4" pageNumber="35">N</emphasis>
posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3;
<emphasis id="B964D70EFFB4FFF0FBAD3C46FBBE1A12" bold="true" box="[1092,1111,1797,1819]" pageId="4" pageNumber="35">O</emphasis>
anterior region, arrowhead shows the anterior part of membranelle 2;
<emphasis id="B964D70EFFB4FFF0FE083C62FE191A3F" bold="true" box="[481,496,1825,1846]" pageId="4" pageNumber="35">P</emphasis>
macronucleus;
<emphasis id="B964D70EFFB4FFF0FD753C62FD461A3E" bold="true" box="[668,687,1825,1847]" pageId="4" pageNumber="35">Q</emphasis>
detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm.
</paragraph>
</caption>
<paragraph id="8BAF0B1CFFB5FFF1FF4A3A0AFCEA1CA0" blockId="5.[123,772,223,1940]" pageId="5" pageNumber="36">
The cell swims moderately fast while rotating about main body axis, sometimes lying motionless along substrate such as bottom of Petri dish or detritus (
<figureCitation id="132B1799FFB5FFF1FD7E3ACCFD1F1CA0" box="[663,758,399,425]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Fig. 2D</figureCitation>
).
</paragraph>
<paragraph id="8BAF0B1CFFB5FFF1FF4B3AF1FEBA1FA9" blockId="5.[123,772,223,1940]" pageId="5" pageNumber="36">
There are eighteen or 19 somatic kineties, which are composed of dikinetids in anterior 60% of body and monokinetids in posterior third, extending almost the entire length of the cell, terminating anteriorly at a small glabrous apical plate (
<figureCitation id="132B1799FFB5FFF1FE06397CFD9E1F53" box="[495,631,575,602]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Figs 2E, F</figureCitation>
). There are consistently two preoral kineties to the left of the buccal field (
<figureCitation id="132B1799FFB5FFF1FF5639C5FEAF1FA9" box="[191,326,646,672]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Figs 2G, N</figureCitation>
).
</paragraph>
<paragraph id="8BAF0B1CFFB5FFF1FF4A39EAFD0F1909" blockId="5.[123,772,223,1940]" pageId="5" pageNumber="36">
Oral apparatus is typical for genus: M1 comprises two longitudinal rows of basal bodies, the length of which is about 50% that of the anterior part of M2a (
<figureCitation id="132B1799FFB5FFF1FF6C3851FED31E24" box="[133,314,786,813]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Figs 2G, M, O</figureCitation>
). M2a is double-rowed with its posterior end straight; posterior part of M2b is V-shaped, and is distinctly separated from M2a (
<figureCitation id="132B1799FFB5FFF1FDFF381AFD4D1E7D" box="[534,676,857,884]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Figs 2G, N</figureCitation>
). M3 is three-rowed with a similar length to that of M1 (
<figureCitation id="132B1799FFB5FFF1FD26383FFF221EB0" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Figs 2G, Q</figureCitation>
). Length of paroral membrane is about 70% of body length. Silverline system is typical for the genus with a near-hexagonal honeycomb pattern (
<figureCitation id="132B1799FFB5FFF1FD9538A5FD301909" box="[636,729,998,1024]" captionStart="Fig" captionStartId="4.[132,167,1601,1623]" captionTargetId="figure-6@3.[110,1448,302,1889]" captionTargetPageId="3" captionText="Fig. 2. Pleuronema elegans spec. nov. in vivo (A, B, D, HL), after protargol (EG, MQ) and silver nitrate staining (C). A, H ventral view of a representative individual; B detailed view of cortex to demonstrate arrangement of extrusomes; C part of silverline system; D swimming trace; E, F ventral (E) and dorsal (F) views of the same specimen, showing infraciliature and nuclear apparatus; G detailed structure of the buccal area, arrow shows the straight posterior end of M2a; I ventral view, arrow marks paroral membrane and arrowhead shows ingested algae; J ventral view, arrow shows membranelle 1, arrowheads mark the anterior part of membranelle 2; K ventral view, arrowheads point to caudal cilia; L ventral view, arrowheads mark somatic cilia; M infraciliature of ventral side, arrow indicates membranelle 1, arrowhead refers to paired basal bodies; N posterior region, arrow shows V-shaped posterior part of membranelle 2, arrowheads point to preoral kineties, double-arrowhead indicates membranelle 3; O anterior region, arrowhead shows the anterior part of membranelle 2; P macronucleus; Q detailed view of membranelle 3 (arrowhead). M1, 3 membranelles 1 and 3; M2a the anterior part of membranelle 2; M2b the posterior part of membranelle 2; Ma macronucleus; PK preoral kinety; PM paroral membrane. Scale bars: A, E, F, H, I = 50 μm, J = 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8377647" httpUri="https://zenodo.org/record/8377647/files/figure.png" pageId="5" pageNumber="36">Fig. 2C</figureCitation>
).
</paragraph>
<paragraph id="8BAF0B1CFFB5FFF1FF4A3F4AFEA219B8" blockId="5.[123,772,223,1940]" pageId="5" pageNumber="36">
<emphasis id="B964D70EFFB5FFF1FF4A3F4AFE1E192A" bold="true" box="[163,503,1033,1059]" pageId="5" pageNumber="36">SSU rRNA gene sequence:</emphasis>
The SSU rRNA gene sequence of
<taxonomicName id="4C10709FFFB5FFF1FEFE3F6FFDE2194F" authority="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao, 2015" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[279,523,1068,1094]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="elegans" status="spec. nov.">
<emphasis id="B964D70EFFB5FFF1FEFE3F6FFDE2194F" box="[279,523,1068,1094]" italics="true" pageId="5" pageNumber="36">Pleuronema elegans</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A2576A75FFB5FFF1FDFF3F6EFD67194E" box="[534,654,1069,1095]" pageId="5" pageNumber="36" rank="species">spec. nov.</taxonomicNameLabel>
has been deposited in the GenBank database with the accession number, length and G+C content as follows: KF840518, 1661 bp, 42.75%.
</paragraph>
</subSubSection>
<subSubSection id="C30A5897FFB5FFF1FF4A3FFAFAB31C14" pageId="5" pageNumber="36" type="discussion">
<paragraph id="8BAF0B1CFFB5FFF1FF4A3FFAFE96188D" blockId="5.[123,772,223,1940]" pageId="5" pageNumber="36">
<emphasis id="B964D70EFFB5FFF1FF4A3FFAFE1919DA" bold="true" box="[163,496,1209,1235]" pageId="5" pageNumber="36">Remarks and comparison:</emphasis>
Based on its conspicuously pointed posterior end and marine habitat,
<taxonomicName id="4C10709FFFB5FFF1FD2D3F9FFEDF1810" authority="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao, 2015" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="elegans" status="spec. nov.">
<emphasis id="B964D70EFFB5FFF1FD2D3F9FFEDF1810" italics="true" pageId="5" pageNumber="36">Pleuronema elegans</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A2576A75FFB5FFF1FED23E43FE471813" box="[315,430,1280,1306]" pageId="5" pageNumber="36" rank="species">spec. nov.</taxonomicNameLabel>
most resembles two nominal species:
<taxonomicName id="4C10709FFFB5FFF1FF0D3E60FDA61834" authority="Wang et al., 2008" authorityName="Wang" authorityYear="2008" box="[228,591,1314,1341]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="czapikae">
<emphasis id="B964D70EFFB5FFF1FF0D3E60FE851835" box="[228,364,1314,1340]" italics="true" pageId="5" pageNumber="36">P. czapikae</emphasis>
Wang
<emphasis id="B964D70EFFB5FFF1FE2D3E60FE121835" box="[452,507,1314,1340]" italics="true" pageId="5" pageNumber="36">et al</emphasis>
., 2008
</taxonomicName>
and
<taxonomicName id="4C10709FFFB5FFF1FD663E60FE731869" authority="Czapik &amp; Jordan, 1977" authorityName="Czapik &amp; Jordan" authorityYear="1977" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="tardum">
<emphasis id="B964D70EFFB5FFF1FD663E60FCEA1835" box="[655,771,1314,1340]" italics="true" pageId="5" pageNumber="36">P. tardum</emphasis>
<bibRefCitation id="EF8176EDFFB5FFF1FF943E05FE731869" author="Czapik A. &amp; Jordan A." box="[125,410,1350,1376]" pageId="5" pageNumber="36" pagination="157 - 164" refId="ref6929" refString="Czapik A., Jordan A. (1977) Two new psammobiotic ciliates Hippocomos loricatus gen. n. sp. and Pleuronema tardum n. sp. (Hymenostomata, Pleuronematina). Acta Protozool. 16: 157 - 164" type="journal article" year="1977">Czapik &amp; Jordan, 1977</bibRefCitation>
</taxonomicName>
and can be readily separated from other congeners.
</paragraph>
<paragraph id="8BAF0B1CFFB5FFF1FF4A3ECFFE871A0E" blockId="5.[123,772,223,1940]" pageId="5" pageNumber="36">
<taxonomicName id="4C10709FFFB5FFF1FF4A3ECFFE7F18AF" authority="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao, 2015" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[163,406,1420,1446]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="elegans" status="spec. nov.">
<emphasis id="B964D70EFFB5FFF1FF4A3ECFFE7F18AF" box="[163,406,1420,1446]" italics="true" pageId="5" pageNumber="36">Pleuronema elegans</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A2576A75FFB5FFF1FE493ECEFDF118AE" box="[416,536,1421,1447]" pageId="5" pageNumber="36" rank="species">spec. nov.</taxonomicNameLabel>
can be clearly distinguished from
<taxonomicName id="4C10709FFFB5FFF1FEA83EF3FD4918C0" authority="Wang et al., 2008" authorityName="Wang" authorityYear="2008" box="[321,672,1455,1482]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="czapikae">
<emphasis id="B964D70EFFB5FFF1FEA83EF3FE2F18C0" box="[321,454,1455,1481]" italics="true" pageId="5" pageNumber="36">P. czapikae</emphasis>
Wang
<emphasis id="B964D70EFFB5FFF1FDF33EF3FDA418C0" box="[538,589,1455,1481]" italics="true" pageId="5" pageNumber="36">et al</emphasis>
., 2008
</taxonomicName>
through its different body shape (slender oval in outline, with a distinctly pointed posterior end in
<taxonomicName id="4C10709FFFB5FFF1FDBD3EB4FD3A1B19" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[596,723,1526,1552]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="elegans">
<emphasis id="B964D70EFFB5FFF1FDBD3EB4FD3A1B19" box="[596,723,1526,1552]" italics="true" pageId="5" pageNumber="36">P. elegans</emphasis>
</taxonomicName>
vs. elongate-elliptical in outline, almost parallel-sided with both ends slightly pointed in
<taxonomicName id="4C10709FFFB5FFF1FE273D7EFDBB1B5F" box="[462,594,1596,1622]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="czapikae">
<emphasis id="B964D70EFFB5FFF1FE273D7EFDBB1B5F" box="[462,594,1596,1622]" italics="true" pageId="5" pageNumber="36">P. czapikae</emphasis>
</taxonomicName>
), fewer somatic kineties (1819 vs.
<quantity id="4CE8A6F9FFB5FFF1FE633D1CFE1C1B70" box="[394,501,1631,1658]" metricMagnitude="-1" metricUnit="m" metricValue="8.128" metricValueMax="8.89" metricValueMin="7.366" pageId="5" pageNumber="36" unit="in" value="32.0" valueMax="35.0" valueMin="29.0">2935 in</quantity>
<taxonomicName id="4C10709FFFB5FFF1FE163D23FD6C1B70" box="[511,645,1631,1657]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="czapikae">
<emphasis id="B964D70EFFB5FFF1FE163D23FD6C1B70" box="[511,645,1631,1657]" italics="true" pageId="5" pageNumber="36">P. czapikae</emphasis>
</taxonomicName>
) and M2a double-rowed with its posterior end straight (vs. mostly two-rowed but with a short section that is single-rowed, posterior end invariably hook-shaped in
<taxonomicName id="4C10709FFFB5FFF1FD983D89FD131BEA" box="[625,762,1737,1763]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="czapikae">
<emphasis id="B964D70EFFB5FFF1FD983D89FD131BEA" box="[625,762,1737,1763]" italics="true" pageId="5" pageNumber="36">P. czapikae</emphasis>
</taxonomicName>
) (
<bibRefCitation id="EF8176EDFFB5FFF1FF6D3DAFFE8B1A0E" author="Wang Y. &amp; Song W. &amp; Hu X. &amp; Warren A. &amp; Chen X. &amp; Al-Rasheid K. A. S." box="[132,354,1772,1799]" pageId="5" pageNumber="36" pagination="35 - 45" refId="ref9425" refString="Wang Y., Song W., Hu X., Warren A., Chen X., Al-Rasheid K. A. S. (2008 b) Descriptions of two new marine species of Pleuronema, P. czapikae sp. n. and P. wiackowskii sp. n. (Ciliophora: Scuticociliatida), from the Yellow Sea, North China. Acta Protozool. 47: 35 - 45" type="journal article" year="2008">
Wang
<emphasis id="B964D70EFFB5FFF1FF3B3DAEFEEC1A0F" box="[210,261,1772,1798]" italics="true" pageId="5" pageNumber="36">et al</emphasis>
. 2008b
</bibRefCitation>
).
</paragraph>
<paragraph id="8BAF0B1CFFB5FFF1FF4A3C4CFAB31C14" blockId="5.[123,772,223,1940]" lastBlockId="5.[809,1455,223,285]" pageId="5" pageNumber="36">
<taxonomicName id="4C10709FFFB5FFF1FF4A3C4CFE7C1A20" authority="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao, 2015" authorityName="Pan &amp; Huang &amp; Fan &amp; Ma &amp; Al-Rasheid &amp; Miao &amp; Gao" authorityYear="2015" box="[163,405,1807,1833]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="elegans" status="spec. nov.">
<emphasis id="B964D70EFFB5FFF1FF4A3C4CFE7C1A20" box="[163,405,1807,1833]" italics="true" pageId="5" pageNumber="36">Pleuronema elegans</emphasis>
</taxonomicName>
<taxonomicNameLabel id="A2576A75FFB5FFF1FE773C53FDFF1A23" box="[414,534,1808,1834]" pageId="5" pageNumber="36" rank="species">spec. nov.</taxonomicNameLabel>
differs from
<taxonomicName id="4C10709FFFB5FFF1FD513C53FF591A45" authorityName="Czapik &amp; Jordan" authorityYear="1977" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="tardum">
<emphasis id="B964D70EFFB5FFF1FD513C53FF591A45" italics="true" pageId="5" pageNumber="36">P. tardum</emphasis>
</taxonomicName>
in having more preoral kineties (two vs. one in
<taxonomicName id="4C10709FFFB5FFF1FF943C14FF041A79" authorityName="Czapik &amp; Jordan" authorityYear="1977" box="[125,237,1878,1904]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="tardum">
<emphasis id="B964D70EFFB5FFF1FF943C14FF041A79" box="[125,237,1878,1904]" italics="true" pageId="5" pageNumber="36">P. tardum</emphasis>
</taxonomicName>
), less somatic kineties (1819 vs. 4050) and a different ratio of M1 and M3 to the anterior part of M2a (50% vs. M1 and M3 very short and M2a extreme- ly long in
<taxonomicName id="4C10709FFFB5FFF1FC483A40FBF81C15" authorityName="Czapik &amp; Jordan" authorityYear="1977" box="[929,1041,258,284]" class="Oligohymenophorea" family="Pleuronematidae" genus="Pleuronema" kingdom="Chromista" order="Pleuronematida" pageId="5" pageNumber="36" phylum="Ciliophora" rank="species" species="tardum">
<emphasis id="B964D70EFFB5FFF1FC483A40FBF81C15" box="[929,1041,258,284]" italics="true" pageId="5" pageNumber="36">P. tardum</emphasis>
</taxonomicName>
) (
<bibRefCitation id="EF8176EDFFB5FFF1FBC33A41FAA41C14" author="Czapik A. &amp; Jordan A." box="[1066,1357,258,285]" pageId="5" pageNumber="36" pagination="157 - 164" refId="ref6929" refString="Czapik A., Jordan A. (1977) Two new psammobiotic ciliates Hippocomos loricatus gen. n. sp. and Pleuronema tardum n. sp. (Hymenostomata, Pleuronematina). Acta Protozool. 16: 157 - 164" type="journal article" year="1977">
Czapik and
<collectingCountry id="F3074B8CFFB5FFF1FB513A41FAEF1C15" box="[1208,1286,258,284]" name="Jordan" pageId="5" pageNumber="36">Jordan</collectingCountry>
1977
</bibRefCitation>
).
</paragraph>
</subSubSection>
</treatment>
</document>