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<document id="562FE6AC9B1F20A7F1137FFB02C6EE71" ID-DOI="10.5281/zenodo.10396362" ID-ISSN="1942-1354" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1707168658757" checkinUser="felipe" docAuthor="Zhang, Jing, Cong, Qian &amp; Grishin, Nick V." docDate="2023" docId="03810139FFC1BB4FC0CAFB7DE762B329" docLanguage="en" docName="InsectaMundi.2023.1026.1-115.pdf" docOrigin="Insecta Mundi 2023 (26)" docSource="http://dx.doi.org/10.5281/zenodo.10396362" docStyle="DocumentStyle:F9976E9B7F8A094D890004515A7A0592.3:InsectaMundi.2020-.journal_article" docStyleId="F9976E9B7F8A094D890004515A7A0592" docStyleName="InsectaMundi.2020-.journal_article" docStyleVersion="3" docTitle="Myrinia maculosa Zhang &amp; Cong &amp; Grishin 2023, new species" docType="treatment" docVersion="1" lastPageNumber="30" masterDocId="FFB87941FFDFBB50C00AFF56E355B262" masterDocTitle="Supplementary Materials and Appendix" masterLastPageNumber="115" masterPageNumber="1" pageNumber="29" updateTime="1707169288913" updateUser="GgImagineBatch" zenodo-license-document="CC-BY-NC-3.0">
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<mods:title id="E7A7401CDBFD69C7431415BB42BA5016">Supplementary Materials and Appendix</mods:title>
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<mods:name id="35629A20759EC0560F1F791692E59271" type="personal">
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<mods:namePart id="F267B48736471FA8823ADF4FA0FC5668">Zhang, Jing</mods:namePart>
<mods:affiliation id="CF6A2640FC38D1DD13DDC5EAF08E7E27">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="C5C712924A3399603AAA4443864A0198">Cong, Qian</mods:namePart>
<mods:affiliation id="E4C252D41CDB1F5329D16F0858DC17C3">McDermott Center for Human Growth and Development and Department of Biophysics University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 8816 USA</mods:affiliation>
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<mods:namePart id="A235801F84BE3B29E1F98027534F13FE">Grishin, Nick V.</mods:namePart>
<mods:affiliation id="F32CF21AFCA0395B90B9DD7566F6552F">Departments of Biophysics and Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Blvd., Dallas, TX, 75390 - 9050 USA</mods:affiliation>
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<mods:title id="C47DDCD2171DE7D5F7968514A7F19FA7">Insecta Mundi</mods:title>
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<mods:date id="5B6A58437238E69022526B856E6CC81A">2023</mods:date>
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<treatment id="03810139FFC1BB4FC0CAFB7DE762B329" LSID="urn:lsid:plazi:treatment:03810139FFC1BB4FC0CAFB7DE762B329" httpUri="http://treatment.plazi.org/id/03810139FFC1BB4FC0CAFB7DE762B329" lastPageId="31" lastPageNumber="30" pageId="30" pageNumber="29">
<subSubSection id="C332E3A4FFC1BB4EC0CAFB7DE0F0B608" pageId="30" pageNumber="29" type="nomenclature">
<paragraph id="8B97B02FFFC1BB4EC0CAFB7DE1E6B62A" blockId="30.[192,933,1067,1164]" box="[192,691,1067,1096]" pageId="30" pageNumber="29">
<heading id="D0DF0743FFC1BB4EC0CAFB7DE1E6B62A" box="[192,691,1067,1096]" fontSize="12" level="2" pageId="30" pageNumber="29" reason="2">
<emphasis id="B95C6C3DFFC1BB4EC0CAFB7DE1E6B62A" bold="true" box="[192,691,1067,1096]" pageId="30" pageNumber="29">
<taxonomicName id="4C28CBACFFC1BB4EC0CAFB7DE147B62A" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[192,530,1067,1096]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="30" pageNumber="29" phylum="Arthropoda" rank="species" species="maculosa" status="sp. nov.">
<emphasis id="B95C6C3DFFC1BB4EC0CAFB7DE2F3B62A" bold="true" box="[192,422,1067,1096]" italics="true" pageId="30" pageNumber="29">Myrinia maculosa</emphasis>
Grishin
</taxonomicName>
,
<taxonomicNameLabel id="A26FD146FFC1BB4EC214FB7AE1E6B62A" box="[542,691,1067,1096]" pageId="30" pageNumber="29" rank="species">new species</taxonomicNameLabel>
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https://zoobank.org/
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<subSubSection id="C332E3A4FFC1BB4EC0CAFB24E156B6EE" box="[192,515,1137,1164]" pageId="30" pageNumber="29" type="description">
<paragraph id="8B97B02FFFC1BB4EC0CAFB24E156B6EE" blockId="30.[192,933,1067,1164]" box="[192,515,1137,1164]" pageId="30" pageNumber="29">
(
<figureCitation id="1313ACAAFFC1BB4EC0C2FB24E252B6EE" box="[200,263,1138,1164]" captionStart="Figure 2" captionStartId="20.[192,261,1419,1443]" captionTargetBox="[193,1440,268,1379]" captionTargetId="figure-157@20.[192,1440,266,1382]" captionTargetPageId="20" captionText="Figure 2. Phylogenetic trees of Achlyodini inferred from protein-coding regions in a) the Z chromosome and b) the mitochondrial genome (in segments). See Fig. 1 legend for other notations." pageId="30" pageNumber="29">Fig. 2</figureCitation>
part, 6566, 279281)
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFC1BB4EC0CAFBCDE7D8B4AB" pageId="30" pageNumber="29" type="diagnosis">
<paragraph id="8B97B02FFFC1BB4EC0CAFBCDE23FB78A" blockId="30.[192,1442,1179,1512]" pageId="30" pageNumber="29">
<emphasis id="B95C6C3DFFC1BB4EC0CAFBCDE2A5B6D7" bold="true" box="[192,496,1179,1206]" pageId="30" pageNumber="29">Definition and diagnosis.</emphasis>
Phylogenetic analysis reveals that a specimen from
<collectingCountry id="F33FF0BFFFC1BB4EC45AFBCAE79CB6D4" box="[1104,1225,1180,1206]" name="Guatemala" pageId="30" pageNumber="29">Guatemala</collectingCountry>
identified as
<taxonomicName id="4C28CBACFFC1BB4EC563FBCAE2B2B6BA" authority="(Mabille, 1898)" baseAuthorityName="Mabille" baseAuthorityYear="1898" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="30" pageNumber="29" phylum="Arthropoda" rank="species" species="myris">
<emphasis id="B95C6C3DFFC1BB4EC563FBCAE267B6BA" italics="true" pageId="30" pageNumber="29">Myrinia myris</emphasis>
(Mabille, 1898)
</taxonomicName>
(
<typeStatus id="54930E8DFFC1BB4EC1FDFBE8E17CB6BA" box="[503,553,1214,1240]" pageId="30" pageNumber="29">type</typeStatus>
locality in
<collectingCountry id="F33FF0BFFFC1BB4EC2A6FBE8E1A4B6BA" box="[684,753,1214,1240]" name="Brazil" pageId="30" pageNumber="29">Brazil</collectingCountry>
:
<taxonomicName id="4C28CBACFFC1BB4EC2F6FBE8E0F0B6BA" authority="Catarina" authorityName="Catarina" box="[764,933,1214,1240]" class="Insecta" family="Hesperiidae" genus="Nisoniades" kingdom="Animalia" order="Lepidoptera" pageId="30" pageNumber="29" phylum="Arthropoda" rank="species" species="santa">Santa Catarina</taxonomicName>
) is genetically differentiated from it (
<figureCitation id="1313ACAAFFC1BB4EC544FBE8E6C7B6BA" box="[1358,1426,1214,1240]" captionStart="Figure 2" captionStartId="20.[192,261,1419,1443]" captionTargetBox="[193,1440,268,1379]" captionTargetId="figure-157@20.[192,1440,266,1382]" captionTargetPageId="20" captionText="Figure 2. Phylogenetic trees of Achlyodini inferred from protein-coding regions in a) the Z chromosome and b) the mitochondrial genome (in segments). See Fig. 1 legend for other notations." pageId="30" pageNumber="29">Fig. 2</figureCitation>
): e.g., their COI barcodes differ by 3.2% (21 bp), and therefore represents a new species. The new species keys to
<taxonomicName id="4C28CBACFFC1BB4EC0EAFA55E212B77E" baseAuthorityName="Mabille" baseAuthorityYear="1898" box="[224,327,1283,1308]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="30" pageNumber="29" phylum="Arthropoda" rank="species" species="myris">
<emphasis id="B95C6C3DFFC1BB4EC0EAFA55E212B77E" box="[224,327,1283,1308]" italics="true" pageId="30" pageNumber="29">M. myris</emphasis>
</taxonomicName>
(E.24.2) in
<bibRefCitation id="EFB9CDDEFFC1BB4EC1DEFA54E13DB77F" author="Evans WH" box="[468,616,1282,1309]" pageId="30" pageNumber="29" pagination="26 - 53" refId="ref77110" refString="Evans WH. 1953. A catalogue of the American Hesperiidae indicating the classification and nomenclature adopted in the British Museum (Natural History). Part III. Pyrginae. Section 2. The Trustees of the British Museum (Natural History); London. v + 246 p., pl. 26 - 53." type="book chapter" year="1953">Evans (1953)</bibRefCitation>
and differs from it by more contrasting wing patterns: dark bands and spots stand out on paler ground color stronger than in
<taxonomicName id="4C28CBACFFC1BB4EC34BFA73E0FDB75C" baseAuthorityName="Mabille" baseAuthorityYear="1898" box="[833,936,1317,1342]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="30" pageNumber="29" phylum="Arthropoda" rank="species" species="myris">
<emphasis id="B95C6C3DFFC1BB4EC34BFA73E0FDB75C" box="[833,936,1317,1342]" italics="true" pageId="30" pageNumber="29">M. myris</emphasis>
</taxonomicName>
, e.g., forewing subapical dark spots. Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly528.33.1:T420A, aly707.3.1:G46A, aly315.8.2:G129C, aly536.208.9:A36G, aly4645.9.3:T87A, aly728.25.1:T72T (not A), aly
<date id="FF9696EFFFC1BB4EC3B5FADCE775B7C7" box="[959,1056,1418,1445]" pageId="30" pageNumber="29" value="1475-03-02">1475.3.2</date>
:G1077G (not A), aly600.8.1:G81G (not A), aly1720.13.9:C206C (not A), aly1350.21.3:T54T (not C), and COI barcode: A208G, A238A, T287C, A298T, T601C.
</paragraph>
<paragraph id="8B97B02FFFC1BB4EC0CAFAAFE7D8B4AB" blockId="30.[192,1440,1528,1737]" pageId="30" pageNumber="29">
<emphasis id="B95C6C3DFFC1BB4EC0CAFAAFE11BB471" bold="true" box="[192,590,1528,1555]" pageId="30" pageNumber="29">
Barcode sequence of the
<typeStatus id="54930E8DFFC1BB4EC1EAFAAFE11CB471" box="[480,585,1529,1555]" pageId="30" pageNumber="29" type="holotype">holotype</typeStatus>
.
</emphasis>
Sample NVG-18061H04, GenBank OR837651, 658 base pairs: AACTTTATATTTTATTTTCGGAATTTGAGCAGGAATAGTTGGAACATCTTTAAGTTTATTAATTCGTACTGAATTAGGTAATCCAGGATCATTAATT GGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGTGGATTTGGTAATT GATTAGTACCTTTGATATTAGGAGCTCCAGATATAGCTTTCCCACGAATAAATAATATAAGATTTTGACTTTTACCTCCATCTTTAATATTACTAAT TTCAAGTAGAATTGTAGAAAATGGAGCAGGAACAGGATGAACTGTTTATCCTCCTTTATCAGCTAATATTGCTCATCAAGGATCTTCTGTAGATTTA GCTATTTTTTCTCTACATTTAGCAGGAATTTCTTCAATTTTAGGAGCTATTAATTTTATTACAACAATTATTAATATACGTATTAATAACTTATCAT TTGATCAAATACCATTATTTGTATGAGCAGTAGGAATTACAGCTTTATTATTATTATTATCTTTACCTGTATTAGCAGGTGCTATTACTATACTTTT AACAGATCGAAATTTAAACACATCTTTTTTTGATCCAGCTGGAGGAGGAGATCCTATTTTATATCAACATTTATTT
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFC1BB4EC0CAF98AE0C3B5E4" pageId="30" pageNumber="29" type="materials_examined">
<paragraph id="8B97B02FFFC1BB4EC0CAF98AE67DB53F" blockId="30.[191,1441,1755,1887]" pageId="30" pageNumber="29">
<emphasis id="B95C6C3DFFC1BB4EC0CAF98AE239B497" bold="true" box="[192,364,1755,1782]" pageId="30" pageNumber="29">Type material.</emphasis>
<materialsCitation id="3B40BA72FFC1BB4EC17FF98AE67DB53F" collectionCode="DC, USNM, V" collectorName="Grishin &amp; QR Code" country="Guatemala" location="National Museum of Natural History" municipality="Smithsonian Institution" pageId="30" pageNumber="29" specimenCount="2" specimenCount-male="2" stateProvince="Retalhuleu" typeStatus="holotype">
<emphasis id="B95C6C3DFFC1BB4EC17FF98AE2BCB494" bold="true" box="[373,489,1756,1782]" pageId="30" pageNumber="29">
<typeStatus id="54930E8DFFC1BB4EC17FF98AE2B1B494" box="[373,484,1756,1782]" pageId="30" pageNumber="29" type="holotype">Holotype</typeStatus>
:
</emphasis>
<specimenCount id="9D2E7BA6FFC1BB4EC1F9F98BE15DB49B" box="[499,520,1757,1785]" count="1" pageId="30" pageNumber="29" type="male"></specimenCount>
deposited in the
<location id="8EF7E6F4FFC1BB4EC2DFF98AE72AB494" LSID="urn:lsid:plazi:treatment:03810139FFC1BB4FC0CAFB7DE762B329:8EF7E6F4FFC1BB4EC2DFF98AE72AB494" box="[725,1151,1756,1782]" country="Guatemala" municipality="Smithsonian Institution" name="National Museum of Natural History" pageId="30" pageNumber="29" stateProvince="Retalhuleu">National Museum of Natural History</location>
,
<collectingMunicipality id="6BF32A55FFC1BB4EC486F98AE6C9B494" box="[1164,1436,1756,1782]" pageId="30" pageNumber="29">Smithsonian Institution</collectingMunicipality>
, Washington,
<collectionCode id="ED3928EAFFC1BB4EC159F9A8E228B57A" box="[339,381,1790,1816]" pageId="30" pageNumber="29">DC</collectionCode>
, USA (
<collectionCode id="ED3928EAFFC1BB4EC1C3F9A8E14BB57A" box="[457,542,1790,1816]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:34871" lsid="urn:lsid:biocol.org:col:34871" name="Smithsonian Institution, National Museum of Natural History" pageId="30" pageNumber="29" type="Museum">USNM</collectionCode>
), illustrated in
<figureCitation id="1313ACAAFFC1BB4EC2CBF9A8E062B57B" box="[705,823,1790,1817]" captionStart="Figures 6384" captionStartId="98.[192,271,1810,1834]" captionTargetBox="[196,1434,233,1782]" captionTargetId="figure-99@98.[192,1440,229,1789]" captionTargetPageId="98" captionText="Figures 6384. Holotypes, data in text. 6364) Myrinia aragua sp. n. 6566) Myrinia maculosa sp. n. 6768) Myrinia manchada sp. n. 6970) Polyctor (Fenops) lamperus sp. n. 7172) Nisoniades (Nisoniades) lutum sp. n. 7374) Bolla (Stolla) vena sp. n. 7576) Staphylus (Vulga) vula sp. n. 7778) Staphylus (Vulga) vulga sp. n. 7980) Staphylus (Staphylus) rotundalus sp. n. 8182) Staphylus (Staphylus) yucatanus sp. n. 8384) Heliopetes (Heliopetes) lana sp. n." pageId="30" pageNumber="29">Fig. 6566</figureCitation>
, bears the following four rectangular labels, three white: [fromLThiel | SSebastian |
<collectingRegion id="49EC7ECDFFC1BB4EC1E3F876E137B558" box="[489,610,1824,1850]" country="Guatemala" name="Retalhuleu" pageId="30" pageNumber="29">Retalhuleu</collectingRegion>
|
<collectingCountry id="F33FF0BFFFC1BB4EC27FF876E1BBB558" box="[629,750,1824,1850]" name="Guatemala" pageId="30" pageNumber="29">Guatemala</collectingCountry>
], [DNA sample ID: | NVG-18061H04 | c/o Nick
<collectionCode id="ED3928EAFFC1BB4EC512F876E67EB558" box="[1304,1323,1824,1850]" country="Canada" lsid="urn:lsid:biocol.org:col:13946" name="Royal British Columbia Museum - Herbarium" pageId="30" pageNumber="29" type="Museum">V</collectionCode>
.
<collectorName id="26DDD5F9FFC1BB4EC53EF876E6DFB558" box="[1332,1418,1824,1850]" pageId="30" pageNumber="29">Grishin</collectorName>
], [USNMENT | {
<collectorName id="26DDD5F9FFC1BB4EC17AF814E288B53E" box="[368,477,1858,1884]" pageId="30" pageNumber="29">QR Code</collectorName>
} | 01466905], and one red [
<typeStatus id="54930E8DFFC1BB4EC316F814E0F8B53E" box="[796,941,1858,1884]" pageId="30" pageNumber="29" type="holotype">HOLOTYPE</typeStatus>
<specimenCount id="9D2E7BA6FFC1BB4EC3BEF815E09CB53D" box="[948,969,1859,1887]" count="1" pageId="30" pageNumber="29" type="male"></specimenCount>
|
<taxonomicName id="4C28CBACFFC1BB4EC3D7F814E762B53E" authorityName="Evans" authorityYear="1953" box="[989,1079,1858,1884]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="30" pageNumber="29" phylum="Arthropoda" rank="genus">Myrinia</taxonomicName>
|
<taxonomicName id="4C28CBACFFC1BB4EC441F814E647B53E" authority="Grishin" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[1099,1298,1858,1884]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="30" pageNumber="29" phylum="Arthropoda" rank="species" species="maculosa">maculosa Grishin</taxonomicName>
].
</materialsCitation>
</paragraph>
<paragraph id="8B97B02FFFC1BB4EC0CAF83AE0C3B5E4" blockId="30.[192,918,1900,1926]" box="[192,918,1900,1926]" pageId="30" pageNumber="29">
<emphasis id="B95C6C3DFFC1BB4EC0CAF83AE20EB5E4" bold="true" box="[192,347,1900,1926]" pageId="30" pageNumber="29">
<typeStatus id="54930E8DFFC1BB4EC0CAF83AE3ACB5E4" box="[192,249,1900,1926]" pageId="30" pageNumber="29">Type</typeStatus>
locality.
</emphasis>
<collectingCountry id="F33FF0BFFFC1BB4EC16BF83AE288B5E4" box="[353,477,1900,1926]" name="Guatemala" pageId="30" pageNumber="29">Guatemala</collectingCountry>
:
<collectingRegion id="49EC7ECDFFC1BB4EC1EDF83AE1B9B5E4" box="[487,748,1900,1926]" country="Guatemala" name="Retalhuleu" pageId="30" pageNumber="29">Retalhuleu department</collectingRegion>
, San Sebastián.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFC0BB4FC0CAFFB0E797B343" pageId="31" pageNumber="30" type="etymology">
<paragraph id="8B97B02FFFC0BB4FC0CAFFB0E797B343" blockId="31.[192,1440,230,290]" pageId="31" pageNumber="30">
<emphasis id="B95C6C3DFFC0BB4FC0CAFFB0E211B362" bold="true" box="[192,324,230,256]" pageId="31" pageNumber="30">Etymology.</emphasis>
In Latin,
<taxonomicName id="4C28CBACFFC0BB4FC1B9FFB0E14EB362" authorityName="Zhang &amp; Cong &amp; Grishin" authorityYear="2023" box="[435,539,230,256]" class="Insecta" family="Hesperiidae" genus="Myrinia" kingdom="Animalia" order="Lepidoptera" pageId="31" pageNumber="30" phylum="Arthropoda" rank="species" species="maculosa">
<emphasis id="B95C6C3DFFC0BB4FC1B9FFB0E14EB362" box="[435,539,230,256]" italics="true" pageId="31" pageNumber="30">maculosa</emphasis>
</taxonomicName>
means spotted, speckled, dappled, mottled, or variegated. It reflects a more contrasting and spottier wing pattern compared to its closest relatives. The name is an adjective.
</paragraph>
</subSubSection>
<subSubSection id="C332E3A4FFC0BB4FC0CAFE67E762B329" box="[192,1079,305,331]" pageId="31" pageNumber="30" type="distribution">
<paragraph id="8B97B02FFFC0BB4FC0CAFE67E762B329" blockId="31.[192,1079,305,331]" box="[192,1079,305,331]" pageId="31" pageNumber="30">
<emphasis id="B95C6C3DFFC0BB4FC0CAFE67E20EB329" bold="true" box="[192,347,305,331]" pageId="31" pageNumber="30">Distribution.</emphasis>
Currently known only from the
<typeStatus id="54930E8DFFC0BB4FC2CDFE67E07CB329" box="[711,809,305,331]" pageId="31" pageNumber="30" type="holotype">holotype</typeStatus>
collected in
<collectingCountry id="F33FF0BFFFC0BB4FC3BDFE67E766B329" box="[951,1075,305,331]" name="Guatemala" pageId="31" pageNumber="30">Guatemala</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
</document>