192 lines
16 KiB
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192 lines
16 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.75.57192" ID-GBIF-Dataset="70ebf204-5c89-4782-8357-853357218fdd" ID-PMC="PMC7669824" ID-Pensoft-Pub="1314-4049-75-31" ID-Pensoft-UUID="C7E17CB78AA959F4AC1E9E9A425599B2" ID-PubMed="33223920" ModsDocID="1314-4049-75-31" checkinTime="1604986494284" checkinUser="pensoft" docAuthor="Lv, Shi-Long, Chai, Chun-Yue, Wang, Yun, Yan, Zhen-Li & Hui, Feng-Li" docDate="2020" docId="3FC2579255DD5C8496FCB8FFA2876FD7" docLanguage="en" docName="MycoKeys 75: 31-49" docOrigin="MycoKeys 75" docSource="http://dx.doi.org/10.3897/mycokeys.75.57192" docTitle="Spathaspora elongata C. Y. Chai & F. L. Hui 2020, sp. nov." docType="treatment" docVersion="4" id="C7E17CB78AA959F4AC1E9E9A425599B2" lastPageNumber="31" masterDocId="C7E17CB78AA959F4AC1E9E9A425599B2" masterDocTitle="Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China" masterLastPageNumber="49" masterPageNumber="31" pageNumber="31" updateTime="1668136577485" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>Five new additions to the genus Spathaspora (Saccharomycetales, Debaryomycetaceae) from southwest China</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Lv, Shi-Long</mods:namePart>
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<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Chai, Chun-Yue</mods:namePart>
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<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Wang, Yun</mods:namePart>
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<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Yan, Zhen-Li</mods:namePart>
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<mods:affiliation>State Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Enterprise Group Co. Ltd., Nanyang 473000, China</mods:affiliation>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hui, Feng-Li</mods:namePart>
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<mods:affiliation>School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China</mods:affiliation>
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<mods:nameIdentifier type="email">fenglihui@yeah.net</mods:nameIdentifier>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2020</mods:date>
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<mods:detail type="volume">
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<mods:number>75</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>31</mods:start>
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<mods:end>49</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.75.57192</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.75.57192</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-75-31</mods:identifier>
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<mods:identifier type="Pensoft-UUID">C7E17CB78AA959F4AC1E9E9A425599B2</mods:identifier>
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<treatment ID-GBIF-Taxon="168961854" LSID="urn:lsid:plazi:treatment:3FC2579255DD5C8496FCB8FFA2876FD7" httpUri="http://treatment.plazi.org/id/3FC2579255DD5C8496FCB8FFA2876FD7" lastPageNumber="31" pageId="0" pageNumber="31">
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<subSubSection pageId="0" pageNumber="31" type="nomenclature">
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<paragraph pageId="0" pageNumber="31">
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<taxonomicName LSID="3FC25792-55DD-5C84-96FC-B8FFA2876FD7" authority="C. Y. Chai & F. L. Hui" authorityName="C. Y. Chai & F. L. Hui" authorityYear="2020" class="Saccharomycetes" family="Debaryomycetaceae" genus="Spathaspora" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spathaspora elongata" order="Saccharomycetales" pageId="0" pageNumber="31" phylum="Ascomycota" rank="species" species="elongata" status="sp. nov.">Spathaspora elongata C.Y. Chai & F.L. Hui</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="31">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Morphology of Spathaspora elongata (NYNU 18115, holotype) a budding cells on YM broth after 3 d b Pseudohyphae on CM agar after 14 d c ascus and ascospore (arrow) on dilute V 8 agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure2" httpUri="https://binary.pensoft.net/fig/473337" pageId="0" pageNumber="31">Figure 2</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="materials_examined">
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<paragraph pageId="0" pageNumber="31">Type.</paragraph>
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<paragraph pageId="0" pageNumber="31">China, Yunnan Province, Jinghong City, Mengyang Town, in rotting wood from a tropical rainforest, August 2018, K.F. Liu & Z.W. Xi (holotype, NYNU 18115T preserved in a metabolically-inactive state), ex-holotype: CICC 33353; CBS 16002.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="etymology">
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<paragraph pageId="0" pageNumber="31">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="31">
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<taxonomicName class="Magnoliopsida" family="Asteraceae" genus="Elongata" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Elongata" order="Asterales" pageId="0" pageNumber="31" phylum="Tracheophyta" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="31">Elongata</emphasis>
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</taxonomicName>
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refers to the elongate ascospores of this yeast.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="description">
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<paragraph pageId="0" pageNumber="31">Description.</paragraph>
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<paragraph pageId="0" pageNumber="31">
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After 3 days of culture in YM broth at 25 °C, the cells are ovoid (3-4
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<normalizedToken originalValue="×">x</normalizedToken>
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3-7
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<normalizedToken originalValue="μm">μm</normalizedToken>
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) and occur singly or in pairs (Fig.
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Morphology of Spathaspora elongata (NYNU 18115, holotype) a budding cells on YM broth after 3 d b Pseudohyphae on CM agar after 14 d c ascus and ascospore (arrow) on dilute V 8 agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure2" httpUri="https://binary.pensoft.net/fig/473337" pageId="0" pageNumber="31">2a</figureCitation>
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). Budding is multilateral. Sediment is formed after a month, but a pellicle is not observed. After 3 days of growth on YM agar at 25 °C, colonies are white to cream-coloured, butyrous and smooth with entire margins. After 14 days at 25 °C, on Dalmau plate culture on CM agar, pseudohyphae are present, but true hyphae are not formed (Fig.
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Morphology of Spathaspora elongata (NYNU 18115, holotype) a budding cells on YM broth after 3 d b Pseudohyphae on CM agar after 14 d c ascus and ascospore (arrow) on dilute V 8 agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure2" httpUri="https://binary.pensoft.net/fig/473337" pageId="0" pageNumber="31">2b</figureCitation>
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). Sporulation occurs on dilute (1:19) V8 agar after 14 days at 25 °C. Unconjugated asci are formed from single cells with one elongated ascospore which are tapered and curved at the ends (Fig.
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<figureCitation captionStart="Figure 2" captionStartId="F2" captionText="Figure 2. Morphology of Spathaspora elongata (NYNU 18115, holotype) a budding cells on YM broth after 3 d b Pseudohyphae on CM agar after 14 d c ascus and ascospore (arrow) on dilute V 8 agar after 14 d. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.75.57192.figure2" httpUri="https://binary.pensoft.net/fig/473337" pageId="0" pageNumber="31">2c</figureCitation>
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). Glucose, galactose, maltose and sucrose are weakly fermented. Xylose fermentation is absent using Durham tubes, but ethanol is produced from xylose when determined with alcohol oxidase and peroxidase tests. Glucose, d-ribose, d-xylose, d-arabinose, sucrose, maltose, trehalose, methyl
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<normalizedToken originalValue="α-d-glucoside">α-d-glucoside</normalizedToken>
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, cellobiose, salicin, arbutin, inulin, ribitol, d-glucitol, d-mannitol, 2-keto-d-gluconate, succinate, citrate and ethanol are assimilated. No growth occurs with galactose, l-sorbose, d-glucosamine, l-arabinose, l-rhamnose, melibiose, lactose, raffinose, melezitose, glycerol, erythritol, xylitol, galactitol,
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<emphasis italics="true" pageId="0" pageNumber="31">myo</emphasis>
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-inositol, d-glucono-1, 5-lactone, 5-keto-d-gluconate, d-gluconate, d-glucuronate, dl-lactate or methanol. For the assimilation of nitrogen compounds, growth on ethylamine, l-lysine, glucosamine or d-tryptophan is present, whereas growth on nitrate, nitrite, cadaverine, creatine, creatinine or imidazole is absent. Growth is observed at 37 °C but not at 40 °C. Growth in the presence of 1% acetic acid is present, but growth in the presence of 10% sodium chloride (NaCl) plus 5% glucose and 0.01% cycloheximide is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.
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</paragraph>
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<caption doi="10.3897/mycokeys.75.57192.figure2" httpUri="https://binary.pensoft.net/fig/473337" pageId="0" pageNumber="31" start="Figure 2" startId="F2">
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<paragraph pageId="0" pageNumber="31">
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<emphasis bold="true" pageId="0" pageNumber="31">Figure 2.</emphasis>
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Morphology of
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<taxonomicName authorityName="C. Y. Chai & F. L. Hui" authorityYear="2020" class="Saccharomycetes" family="Saccharomycetaceae" genus="Spathaspora" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spathaspora elongata" order="Saccharomycetales" pageId="0" pageNumber="31" phylum="Ascomycota" rank="species" species="elongata">
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<emphasis italics="true" pageId="0" pageNumber="31">Spathaspora elongata</emphasis>
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</taxonomicName>
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(NYNU 18115, holotype)
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<emphasis bold="true" pageId="0" pageNumber="31">a</emphasis>
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budding cells on YM broth after 3 d
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<emphasis bold="true" pageId="0" pageNumber="31">b</emphasis>
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Pseudohyphae on CM agar after 14 d
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<emphasis bold="true" pageId="0" pageNumber="31">c</emphasis>
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ascus and ascospore (arrow) on dilute V8 agar after 14 d. Scale bars: 10
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<normalizedToken originalValue="μm">μm</normalizedToken>
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.
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</paragraph>
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</caption>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="additional isolates examined">
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<paragraph pageId="0" pageNumber="31">Additional isolates examined.</paragraph>
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<paragraph pageId="0" pageNumber="31">China, Yunnan Province, Jinghong City, Mengyang Town, in rotting wood from a tropical rainforest, August 2018, K.F. Liu & Z.W. Xi, NYNU 181112, NYNU 181120 and NYNU 181158.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="31" type="notes">
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<paragraph pageId="0" pageNumber="31">Notes.</paragraph>
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<paragraph pageId="0" pageNumber="31">
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Four strains, representing
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<taxonomicName lsidName="Sp. elongata" pageId="0" pageNumber="31" rank="species" species="elongata">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. elongata</emphasis>
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</taxonomicName>
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, clustered in a well-supported phylogenetic clade that is closely related to
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<taxonomicName lsidName="Sp. mengyangensis" pageId="0" pageNumber="31" rank="species" species="mengyangensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. mengyangensis</emphasis>
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</taxonomicName>
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, another new species proposed in this paper and
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<taxonomicName lsidName="C. subhashii" pageId="0" pageNumber="31" rank="species" species="subhashii">
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<emphasis italics="true" pageId="0" pageNumber="31">C. subhashii</emphasis>
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</taxonomicName>
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. The nucleotide differences between
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<taxonomicName lsidName="Sp. elongata" pageId="0" pageNumber="31" rank="species" species="elongata">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. elongata</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="Sp. mengyangensis" pageId="0" pageNumber="31" rank="species" species="mengyangensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. mengyangensis</emphasis>
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</taxonomicName>
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were 2.5% substitutions in the D1/D2 domain and 5.2% substitutions in the ITS region (
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<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2016.11.007" author="Groenewald, DVM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="31" pagination="91 - 105" refId="B9" refString="Groenewald, DVM, Szoeke, S, Cardinali, G, Eberhardt, U, Stielow, B, Vries, M de, Verkleij, MJM, Crous, PW, Boekhout, T, Robert, V, 2016. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Studies in Mycology 85: 91 - 105, DOI: https://doi.org/10.1016/j.simyco.2016.11.007" title="DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation." url="https://doi.org/10.1016/j.simyco.2016.11.007" volume="85" year="2016">Groenewald et al. 2016</bibRefCitation>
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). Similarly,
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<taxonomicName lsidName="Sp. elongata" pageId="0" pageNumber="31" rank="species" species="elongata">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. elongata</emphasis>
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</taxonomicName>
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and
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<taxonomicName lsidName="C. subhashii" pageId="0" pageNumber="31" rank="species" species="subhashii">
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<emphasis italics="true" pageId="0" pageNumber="31">C. subhashii</emphasis>
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</taxonomicName>
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showed differences of 3.9% substitutions in the D1/D2 domain and 5.9% substitutions in the ITS region (
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<bibRefCitation DOI="https://doi.org/10.1016/j.simyco.2016.11.007" author="Groenewald, DVM" journalOrPublisher="Studies in Mycology" pageId="0" pageNumber="31" pagination="91 - 105" refId="B9" refString="Groenewald, DVM, Szoeke, S, Cardinali, G, Eberhardt, U, Stielow, B, Vries, M de, Verkleij, MJM, Crous, PW, Boekhout, T, Robert, V, 2016. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Studies in Mycology 85: 91 - 105, DOI: https://doi.org/10.1016/j.simyco.2016.11.007" title="DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation." url="https://doi.org/10.1016/j.simyco.2016.11.007" volume="85" year="2016">Groenewald et al. 2016</bibRefCitation>
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). Physiologically,
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<taxonomicName lsidName="Sp. elongata" pageId="0" pageNumber="31" rank="species" species="elongata">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. elongata</emphasis>
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</taxonomicName>
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can be differentiated from its close relative,
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<taxonomicName lsidName="Sp. mengyangensis" pageId="0" pageNumber="31" rank="species" species="mengyangensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. mengyangensis</emphasis>
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</taxonomicName>
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, based on its growth in citrate and the presence of 1% acetic acid, which are present for
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<taxonomicName lsidName="Sp. elongata" pageId="0" pageNumber="31" rank="species" species="elongata">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. elongata</emphasis>
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</taxonomicName>
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and absent for
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<taxonomicName lsidName="Sp. mengyangensis" pageId="0" pageNumber="31" rank="species" species="mengyangensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. mengyangensis</emphasis>
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</taxonomicName>
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. Moreover,
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<taxonomicName lsidName="Sp. elongata" pageId="0" pageNumber="31" rank="species" species="elongata">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. elongata</emphasis>
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</taxonomicName>
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weakly ferments glucose, galactose, maltose and sucrose and grows at 37 °C, but
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<taxonomicName lsidName="Sp. mengyangensis" pageId="0" pageNumber="31" rank="species" species="mengyangensis">
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<emphasis italics="true" pageId="0" pageNumber="31">Sp. mengyangensis</emphasis>
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</taxonomicName>
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does not.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |