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115 KiB
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433 lines
115 KiB
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<document id="54D99C6AECA79B3BBDE6BDA3627307C7" ID-DOI="10.11646/phytotaxa.277.1.1" ID-ISSN="1179-3163" ID-Zenodo-Dep="13652678" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1725383090839" checkinUser="felipe" docAuthor="Gabriel, Daniela, Draisma, Stefano G. A., Sauvage, Thomas, Schmidt, William E., Schils, Tom, Lim, Phaik-Eem, Harris, D. James & Fredericq, Suzanne" docDate="2016" docId="03ED87E31B5FFFB8FF306F74FA80FC58" docLanguage="en" docName="phytotaxa.277.1.1.pdf" docOrigin="Phytotaxa 277 (1)" docSource="http://dx.doi.org/10.11646/phytotaxa.277.1.1" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.13:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="13" docTitle="Gibsmithia eilatensis Gabriel and Fredericq 2016, sp. nov." docType="treatment" docVersion="2" lastPageNumber="17" masterDocId="FFD4FF9B1B55FFA8FFB86873FFC0FFD1" masterDocTitle="Multilocus phylogeny reveals Gibsmithia hawaiiensis (Dumontiaceae, Rhodophyta) to be a species complex from the Indo-Pacific, with the proposal of G. eilatensis sp. nov." masterLastPageNumber="20" masterPageNumber="1" pageNumber="11" updateTime="1725394376524" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="A714487C7D21B42A54B526C0D656E9CE">Multilocus phylogeny reveals Gibsmithia hawaiiensis (Dumontiaceae, Rhodophyta) to be a species complex from the Indo-Pacific, with the proposal of G. eilatensis sp. nov.</mods:title>
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<mods:namePart id="EF2F44CA3ED3A8B6E6AF18253D8C5A1B">Gabriel, Daniela</mods:namePart>
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<mods:affiliation id="13704C12FA01FE1C06E585051A173310">CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Pólo dos Açores - Departamento de Biologia da Universidade dos Açores, R. Mãe de Deus 13 A, 9501 - 801 Ponta Delgada, Portugal & Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504 - 3602, USA</mods:affiliation>
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<mods:namePart id="29DC4A298629A863273523B99431B818">Draisma, Stefano G. A.</mods:namePart>
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<mods:affiliation id="E6BEEF6250C11B86F58B62E0CCC24370">Center of Excellence for Biodiversity of Peninsular Thailand, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand</mods:affiliation>
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<mods:namePart id="0D3990A8DF9576393FBBB614948F60BF">Sauvage, Thomas</mods:namePart>
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<mods:affiliation id="6C750F5DE45FD43C6EFE11F6D375ADCB">Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504 - 3602, USA</mods:affiliation>
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<mods:namePart id="13E952E47207610878C04C6505101C1C">Schmidt, William E.</mods:namePart>
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<mods:affiliation id="8A9F5AC0CF6FBF491F41C79628457ADB">Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504 - 3602, USA</mods:affiliation>
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<mods:namePart id="C3D6A24362E972D40EF0AF80584C4310">Schils, Tom</mods:namePart>
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<mods:affiliation id="6811D6A3525F967A3FACBD380E3C79FE">University of Guam Marine Laboratory, UOG Station, Mangilao, GU 96923, Guam, USA</mods:affiliation>
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<mods:namePart id="D27EB1D6677BFF53EE4FCD0810B89E15">Lim, Phaik-Eem</mods:namePart>
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<mods:affiliation id="E71E92220D288A48D171CD5628A74903">Institute of Ocean and Earth Sciences, University of Malaya, Kuala Lumpur 50603, Malaysia</mods:affiliation>
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<mods:namePart id="7674CD725BAD18369586349FDA2ACEEC">Harris, D. James</mods:namePart>
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<mods:affiliation id="705985A5BAA8E2253E328054245AFB03">CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Pólo dos Açores - Departamento de Biologia da Universidade dos Açores, R. Mãe de Deus 13 A, 9501 - 801 Ponta Delgada, Portugal</mods:affiliation>
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<mods:namePart id="36C4AA91F1A4CC34A183EDCD34E25639">Fredericq, Suzanne</mods:namePart>
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<mods:affiliation id="1747AB9EFC03C828EC226271BE16CD7A">Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504 - 3602, USA</mods:affiliation>
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<mods:title id="36FE71D3854B48EBD3F8E095CA366D4F">Phytotaxa</mods:title>
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<mods:date id="8958B1BD8B6C1A4C940D929AEB481D01">2016</mods:date>
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<taxonomicName id="4C444D761B5FFFA2FF306F74FD4FF8F2" authority="Gabriel and Fredericq" authorityName="Gabriel and Fredericq" authorityYear="2016" box="[136,655,1799,1827]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="10" pageNumber="11" phylum="Rhodophyta" rank="species" species="eilatensis" status="sp. nov.">
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<emphasis id="B930EAE71B5FFFA2FF306F74FEBAF8F3" bold="true" box="[136,378,1799,1826]" italics="true" pageId="10" pageNumber="11">Gibsmithia eilatensis</emphasis>
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Gabriel and Fredericq
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,
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<taxonomicNameLabel id="A203579C1B5FFFA2FD246F7BFD32F8F3" box="[668,754,1800,1826]" pageId="10" pageNumber="11" rank="species">sp. nov.</taxonomicNameLabel>
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<figureCitation id="137F2A701B5FFFA2FF306F5DFEC8F896" box="[136,264,1838,1863]" captionStart="FIGURE 1" captionStartId="1.[136,229,1922,1943]" captionTargetBox="[196,1391,190,1897]" captionTargetId="figure-14@1.[196,1391,190,1897]" captionTargetPageId="1" captionText="FIGURE 1. In situ habits of Gibsmithia hawaiiensis complex.A) DG234, Eilat, Israel. B) SGAD0509563, Kepulauan Seribu, Indonesia. C) SGAD0911460, Ternate, Indonesia. D) TIG001, Pulau Tiga, Malaysia. E) SGAD1205119, Johor, Malaysia. F) SGAD0712185, Raja Ampat, Indonesia. G) SGAD1206005, Labuan, Malaysia. Four individuals, each comprising one stipe and one to a few lobes. H) LEM03, Bitung, Indonesia. Scale bar 1 cm. Photo credits: A) T. Sauvage; B) A. Gittenberger; C, E–G) S.G.A. Draisma; D) M.A.S. Hussein; H) B.T. Reijnen." figureDoi="http://doi.org/10.5281/zenodo.13652682" httpUri="https://zenodo.org/record/13652682/files/figure.png" pageId="10" pageNumber="11">Figures 1A</figureCitation>
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,
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<figureCitation id="137F2A701B5FFFA2FEAB6F5DFEE3F896" box="[275,291,1838,1863]" captionStart="FIGURE 4" captionStartId="11.[136,229,690,711]" captionTargetBox="[170,1423,262,656]" captionTargetId="figure-38@11.[151,1436,242,665]" captionTargetPageId="11" captionText="FIGURE 4. In vivo habits of G. eilatensis sp. nov. Specimens growing: A) attached to coral reef surface (DG219; isotype), B) between coral heads (DG210) and C) under a coral wall (DG234; holotype). Scale bar = 1.0 cm" figureDoi="http://doi.org/10.5281/zenodo.13652692" httpUri="https://zenodo.org/record/13652692/files/figure.png" pageId="10" pageNumber="11">4</figureCitation>
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,
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<figureCitation id="137F2A701B5FFFA2FE976F5DFE99F896" box="[303,345,1838,1863]" captionStart-0="FIGURE 6" captionStart-1="FIGURE 7" captionStart-2="FIGURE 8" captionStartId-0="12.[136,229,1808,1829]" captionStartId-1="13.[136,229,1701,1722]" captionStartId-2="15.[136,229,1808,1829]" captionTargetBox-0="[196,1391,190,1782]" captionTargetBox-1="[228,1359,190,1676]" captionTargetBox-2="[153,1433,572,1779]" captionTargetId-0="figure-15@12.[196,1391,190,1784]" captionTargetId-1="figure-14@13.[228,1359,190,1676]" captionTargetId-2="figure-194@15.[151,1436,569,1782]" captionTargetPageId-0="12" captionTargetPageId-1="13" captionTargetPageId-2="15" captionText-0="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." captionText-1="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." captionText-2="FIGURE 8. Gibsmithia eilatensis sp. nov. A–B) Mature corncob-like spermatangial heads. A) Slide DG220-1. Scale bar 50 μm. B) Slide DG220-1, scale bar 20 μm. C) Young and mature tetrasporangia. Slide DG219-1. Scale bar 20 μm. D–E) Terminal and lateral tetrasporangial initials growing on lateral assimilatory filaments, and decussate and cruciate tetrasporangia. D) Slide DG219-1. Scale bar 50 μm. E) Slide DG219-1. Scale bar 20 μm." figureDoi-0="http://doi.org/10.5281/zenodo.13652698" figureDoi-1="http://doi.org/10.5281/zenodo.13652702" figureDoi-2="http://doi.org/10.5281/zenodo.13652706" httpUri-0="https://zenodo.org/record/13652698/files/figure.png" httpUri-1="https://zenodo.org/record/13652702/files/figure.png" httpUri-2="https://zenodo.org/record/13652706/files/figure.png" pageId="10" pageNumber="11">6–8</figureCitation>
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:—
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.
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:
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N 29
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|
||
<elevation id="0069D1C61B5FFFA2FC226F0BFBC6F85C" box="[922,1030,1912,1933]" metricMagnitude="1" metricUnit="m" metricValue="2.5" pageId="10" pageNumber="11" unit="m" value="25.0">
|
||
<quantity id="4CBC9B101B5FFFA2FC226F0BFC0AF85C" box="[922,970,1912,1933]" metricMagnitude="1" metricUnit="m" metricValue="2.5" pageId="10" pageNumber="11" unit="m" value="25.0">25 m</quantity>
|
||
depth
|
||
</elevation>
|
||
,
|
||
<date id="FFFA10351B5FFFA2FBB66F0BFB49F85C" box="[1038,1161,1912,1933]" pageId="10" pageNumber="11" value="2011-07-24">
|
||
<collectingDate id="EFBEE9DD1B5FFFA2FBB66F0BFB49F85C" box="[1038,1161,1912,1933]" pageId="10" pageNumber="11" value="2011-07-24">24 July 2011</collectingDate>
|
||
</date>
|
||
, DG234, female gametophyte.
|
||
<figureCitation id="137F2A701B5FFFA2FF056FEFFED2F860" box="[189,274,1948,1969]" captionStart="FIGURE 1" captionStartId="1.[136,229,1922,1943]" captionTargetBox="[196,1391,190,1897]" captionTargetId="figure-14@1.[196,1391,190,1897]" captionTargetPageId="1" captionText="FIGURE 1. In situ habits of Gibsmithia hawaiiensis complex.A) DG234, Eilat, Israel. B) SGAD0509563, Kepulauan Seribu, Indonesia. C) SGAD0911460, Ternate, Indonesia. D) TIG001, Pulau Tiga, Malaysia. E) SGAD1205119, Johor, Malaysia. F) SGAD0712185, Raja Ampat, Indonesia. G) SGAD1206005, Labuan, Malaysia. Four individuals, each comprising one stipe and one to a few lobes. H) LEM03, Bitung, Indonesia. Scale bar 1 cm. Photo credits: A) T. Sauvage; B) A. Gittenberger; C, E–G) S.G.A. Draisma; D) M.A.S. Hussein; H) B.T. Reijnen." figureDoi="http://doi.org/10.5281/zenodo.13652682" httpUri="https://zenodo.org/record/13652682/files/figure.png" pageId="10" pageNumber="11">Figs. 1A</figureCitation>
|
||
,
|
||
<figureCitation id="137F2A701B5FFFA2FEA46FEFFEFAF860" box="[284,314,1948,1969]" captionStart="FIGURE 4" captionStartId="11.[136,229,690,711]" captionTargetBox="[170,1423,262,656]" captionTargetId="figure-38@11.[151,1436,242,665]" captionTargetPageId="11" captionText="FIGURE 4. In vivo habits of G. eilatensis sp. nov. Specimens growing: A) attached to coral reef surface (DG219; isotype), B) between coral heads (DG210) and C) under a coral wall (DG234; holotype). Scale bar = 1.0 cm" figureDoi="http://doi.org/10.5281/zenodo.13652692" httpUri="https://zenodo.org/record/13652692/files/figure.png" pageId="10" pageNumber="11">4C</figureCitation>
|
||
,
|
||
<figureCitation id="137F2A701B5FFFA2FEFC6FEFFEBDF860" box="[324,381,1948,1969]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="10" pageNumber="11">6A, T</figureCitation>
|
||
.
|
||
<collectorName id="26B153231B5FFFA2FE3E6FEFFE17F860" box="[390,471,1948,1969]" pageId="10" pageNumber="11">Sauvage</collectorName>
|
||
and
|
||
<collectorName id="26B153231B5FFFA2FDBE6FEFFDADF860" box="[518,621,1948,1969]" pageId="10" pageNumber="11">D. Gabriel</collectorName>
|
||
, deposited in
|
||
<collectionCode id="ED55AE301B5FFFA2FD4A6FEFFCE1F860" box="[754,801,1948,1969]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:14554" lsid="urn:lsid:biocol.org:col:14554" name="University of Louisiana at Lafayette" pageId="10" pageNumber="11" type="Herbarium">LAF</collectionCode>
|
||
.
|
||
</materialsCitation>
|
||
</paragraph>
|
||
<paragraph id="8BFB36F51B5FFFA2FF306FB3FD5EF828" blockId="10.[136,1453,1911,2077]" pageId="10" pageNumber="11">
|
||
<typeStatus id="54FF88571B5FFFA2FF306FB3FF2EF804" box="[136,238,1984,2005]" pageId="10" pageNumber="11" type="isotype">ISOTYPE</typeStatus>
|
||
:—
|
||
<collectingCountry id="F35376651B5FFFA2FEB26FB3FEA1F804" box="[266,353,1984,2005]" name="Israel" pageId="10" pageNumber="11">ISRAEL</collectingCountry>
|
||
. Red Sea: Eilat, Coral Beach,
|
||
<geoCoordinate id="EE7050321B5FFFA2FD3D6FB3FCC8F804" box="[645,776,1983,2005]" degrees="29" direction="north" minutes="30" orientation="latitude" pageId="10" pageNumber="11" precision="15" seconds="14" value="29.50389">
|
||
N 29
|
||
<superScript id="7C319BBD1B5FFFA2FD0D6FCCFD7CF81A" attach="left" box="[693,700,1983,1995]" fontSize="5" pageId="10" pageNumber="11">o</superScript>
|
||
30’ 14”
|
||
</geoCoordinate>
|
||
<geoCoordinate id="EE7050321B5FFFA2FCB66FB3FC50F804" box="[782,912,1983,2005]" degrees="34" direction="east" minutes="55" orientation="longitude" pageId="10" pageNumber="11" precision="15" seconds="09" value="34.919167">
|
||
E 34
|
||
<superScript id="7C319BBD1B5FFFA2FC836FCCFC82F81A" attach="left" box="[827,834,1983,1995]" fontSize="5" pageId="10" pageNumber="11">o</superScript>
|
||
55’ 09”
|
||
</geoCoordinate>
|
||
,
|
||
<quantity id="4CBC9B101B5FFFA2FC226FB3FC7FF804" box="[922,959,1984,2005]" metricMagnitude="0" metricUnit="m" metricValue="5.0" pageId="10" pageNumber="11" unit="m" value="5.0">5 m</quantity>
|
||
depth,
|
||
<date id="FFFA10351B5FFFA2FBB06FB3FB46F804" box="[1032,1158,1984,2005]" pageId="10" pageNumber="11" value="2011-07-22">22 July 2011</date>
|
||
, DG219, tetrasporophyte.
|
||
<figureCitation id="137F2A701B5FFFA2FA3D6FB3FF36F828" captionStart="FIGURE 4" captionStartId="11.[136,229,690,711]" captionTargetBox="[170,1423,262,656]" captionTargetId="figure-38@11.[151,1436,242,665]" captionTargetPageId="11" captionText="FIGURE 4. In vivo habits of G. eilatensis sp. nov. Specimens growing: A) attached to coral reef surface (DG219; isotype), B) between coral heads (DG210) and C) under a coral wall (DG234; holotype). Scale bar = 1.0 cm" figureDoi="http://doi.org/10.5281/zenodo.13652692" httpUri="https://zenodo.org/record/13652692/files/figure.png" pageId="10" pageNumber="11">Fig. 4A, T</figureCitation>
|
||
. Sauvage and D. Gabriel, deposited in
|
||
<collectionCode id="ED55AE301B5FFFA2FDD36F97FD5AF828" box="[619,666,2020,2041]" country="USA" httpUri="http://biocol.org/urn:lsid:biocol.org:col:14554" lsid="urn:lsid:biocol.org:col:14554" name="University of Louisiana at Lafayette" pageId="10" pageNumber="11" type="Herbarium">LAF</collectionCode>
|
||
.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C35E657E1B5FFFA3FF30607BFBD3FF08" lastPageId="11" lastPageNumber="12" pageId="10" pageNumber="11" type="etymology">
|
||
<paragraph id="8BFB36F51B5FFFA3FF30607BFBD3FF08" blockId="10.[136,1453,1911,2077]" lastBlockId="11.[136,1452,160,217]" lastPageId="11" lastPageNumber="12" pageId="10" pageNumber="11">
|
||
<materialsCitation id="3B2C3CA81B5FFFA3FF30607BFBE5FF64" collectorName="Red Sea & Eilat & Coral Beach, DG" country="Israel" lastPageId="11" lastPageNumber="12" location="The" municipality="Red Sea." pageId="10" pageNumber="11" specimenCount="1">
|
||
ETYMOLOGY:
|
||
<location id="8E9B602E1B5FFFA2FE92607BFE8FF7CC" LSID="urn:lsid:plazi:treatment:03ED87E31B5FFFB8FF306F74FA80FC58:8E9B602E1B5FFFA2FE92607BFE8FF7CC" box="[298,335,2056,2077]" country="Israel" municipality="Red Sea." name="The" pageId="10" pageNumber="11">The</location>
|
||
specific epithet refers to the
|
||
<typeStatus id="54FF88571B5FFFA2FDDE607BFD4FF7CC" box="[614,655,2056,2077]" pageId="10" pageNumber="11">type</typeStatus>
|
||
locality in the
|
||
<collectingMunicipality id="6B9FAC8F1B5FFFA2FCA7607BFCB6F7CC" box="[799,886,2056,2077]" pageId="10" pageNumber="11">Red Sea.</collectingMunicipality>
|
||
MATERIAL EXAMINED:—
|
||
<collectingCountry id="F35376651B5EFFA3FE1068D3FE3EFF64" box="[424,510,160,181]" name="Israel" pageId="11" pageNumber="12">ISRAEL</collectingCountry>
|
||
.
|
||
<collectorName id="26B153231B5EFFA3FDB268D3FD9FFF64" box="[522,607,160,181]" pageId="11" pageNumber="12">Red Sea</collectorName>
|
||
:
|
||
<collectorName id="26B153231B5EFFA3FDD268D3FD5AFF64" box="[618,666,160,181]" pageId="11" pageNumber="12">Eilat</collectorName>
|
||
,
|
||
<collectorName id="26B153231B5EFFA3FD1E68D3FC91FF64" box="[678,849,160,181]" pageId="11" pageNumber="12">Coral Beach, DG</collectorName>
|
||
219 (tetrasporophyte)
|
||
</materialsCitation>
|
||
; DG210 (female gametophyte); DG234 (female gametophyte); DG220 (male gametophyte). All specimens are deposited at LAF.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<caption id="DF3B667D1B5EFFA3FF306AC1FC07FD3A" ID-DOI="http://doi.org/10.5281/zenodo.13652692" ID-Zenodo-Dep="13652692" httpUri="https://zenodo.org/record/13652692/files/figure.png" pageId="11" pageNumber="12" startId="11.[136,229,690,711]" targetBox="[170,1423,262,656]" targetPageId="11" targetType="figure">
|
||
<paragraph id="8BFB36F51B5EFFA3FF306AC1FC07FD3A" blockId="11.[136,1451,690,747]" pageId="11" pageNumber="12">
|
||
<emphasis id="B930EAE71B5EFFA3FF306AC1FF3FFD16" bold="true" box="[136,255,690,711]" pageId="11" pageNumber="12">FIGURE 4.</emphasis>
|
||
<taxonomicName id="4C444D761B5EFFA3FEBD6AC1FE88FD16" box="[261,328,690,711]" class="Aves" family="Phasianidae" genus="Indian" kingdom="Animalia" order="Galliformes" pageId="11" pageNumber="12" phylum="Chordata" rank="species" species="vivo">
|
||
<emphasis id="B930EAE71B5EFFA3FEBD6AC1FE88FD16" box="[261,328,690,711]" italics="true" pageId="11" pageNumber="12">In vivo</emphasis>
|
||
</taxonomicName>
|
||
habits of
|
||
<emphasis id="B930EAE71B5EFFA3FE126AC1FDB5FD16" box="[426,629,690,711]" italics="true" pageId="11" pageNumber="12">
|
||
<taxonomicName id="4C444D761B5EFFA3FE126AC1FDE4FD16" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[426,548,690,711]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="11" pageNumber="12" phylum="Rhodophyta" rank="species" species="eilatensis" status="sp. nov.">G. eilatensis</taxonomicName>
|
||
<taxonomicNameLabel id="A203579C1B5EFFA3FD926AC1FDB5FD16" box="[554,629,690,711]" pageId="11" pageNumber="12" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
Specimens growing: A) attached to coral reef surface (DG219; isotype), B) between coral heads (DG210) and C) under a coral wall (DG234; holotype). Scale bar = 1.0 cm
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DF3B667D1B5EFFA3FF306EB7FEC9F8F0" ID-DOI="http://doi.org/10.5281/zenodo.13652696" ID-Zenodo-Dep="13652696" httpUri="https://zenodo.org/record/13652696/files/figure.png" pageId="11" pageNumber="12" startId="11.[136,243,1732,1753]" targetBox="[164,1422,783,1697]" targetPageId="11" targetType="figure">
|
||
<paragraph id="8BFB36F51B5EFFA3FF306EB7FEC9F8F0" blockId="11.[136,1453,1732,1825]" pageId="11" pageNumber="12">
|
||
<emphasis id="B930EAE71B5EFFA3FF306EB7FECBF908" bold="true" box="[136,267,1732,1753]" pageId="11" pageNumber="12">FIGURES 5.</emphasis>
|
||
Range of habits of
|
||
<taxonomicName id="4C444D761B5EFFA3FE716EB7FD6BF908" authorityName="Doty" authorityYear="1963" box="[457,683,1732,1753]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="11" pageNumber="12" phylum="Rhodophyta" rank="species" species="hawaiiensis">
|
||
<emphasis id="B930EAE71B5EFFA3FE716EB7FD6BF908" box="[457,683,1732,1753]" italics="true" pageId="11" pageNumber="12">Gibsmithia hawaiiensis</emphasis>
|
||
</taxonomicName>
|
||
from the Hawaiian Islands (dry herbarium specimens). Main Hawaiian Islands: A) DG261, Hawaii; B) DG255, Hawaii; C) DG253, Oahu; D) DG267, Oahu. Northwestern Hawaiian Islands: E) DG258; F) DG218. Scale bar = 1.0 cm.
|
||
</paragraph>
|
||
</caption>
|
||
<subSubSection id="C35E657E1B5EFFA3FF306F22FCD8F82B" pageId="11" pageNumber="12" type="biology_ecology">
|
||
<paragraph id="8BFB36F51B5EFFA3FF306F22FB2CF863" blockId="11.[136,1452,1873,2042]" pageId="11" pageNumber="12">
|
||
ECOLOGY: Plants growing individually, subtidally to
|
||
<quantity id="4CBC9B101B5EFFA3FCB76F22FC8EF8BB" box="[783,846,1873,1898]" metricMagnitude="1" metricUnit="m" metricValue="2.5" pageId="11" pageNumber="12" unit="m" value="25.0">25 m</quantity>
|
||
depth, usually in cavities of coral colonies in reef environments.
|
||
<taxonomicName id="4C444D761B5EFFA3FE896F06FE00F85F" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[305,448,1909,1934]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="11" pageNumber="12" phylum="Rhodophyta" rank="species" species="eilatensis">
|
||
<emphasis id="B930EAE71B5EFFA3FE896F06FE00F85F" box="[305,448,1909,1934]" italics="true" pageId="11" pageNumber="12">G. eilatensis</emphasis>
|
||
</taxonomicName>
|
||
was the only species of gelatinous red algae observed in the area during the collection period (mid-June to late July). Signs of herbivory or senescence were observed in specimen DG219.
|
||
</paragraph>
|
||
<paragraph id="8BFB36F51B5EFFA3FF056FCDFCD8F82B" blockId="11.[136,1452,1873,2042]" pageId="11" pageNumber="12">
|
||
GEOGRAPHIC DISTRIBUTION:
|
||
<taxonomicName id="4C444D761B5EFFA3FDF56FCEFD1AF807" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[589,730,1981,2006]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="11" pageNumber="12" phylum="Rhodophyta" rank="species" species="eilatensis">
|
||
<emphasis id="B930EAE71B5EFFA3FDF56FCEFD1AF807" box="[589,730,1981,2006]" italics="true" pageId="11" pageNumber="12">G. eilatensis</emphasis>
|
||
</taxonomicName>
|
||
was collected all along the shores of Eilat in the Gulf of Aqaba (Red Sea) and its occurrence outside this area is unknown.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<caption id="DF3B667D1B59FFA4FF306F63FC4DF7F0" ID-DOI="http://doi.org/10.5281/zenodo.13652698" ID-Zenodo-Dep="13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="12" pageNumber="13" startId="12.[136,229,1808,1829]" targetBox="[196,1391,190,1782]" targetPageId="12" targetType="figure">
|
||
<paragraph id="8BFB36F51B59FFA4FF306F63FC4DF7F0" blockId="12.[136,1452,1808,2081]" pageId="12" pageNumber="13">
|
||
<emphasis id="B930EAE71B59FFA4FF306F63FF3EF8F4" bold="true" box="[136,254,1808,1829]" pageId="12" pageNumber="13">FIGURE 6.</emphasis>
|
||
<emphasis id="B930EAE71B59FFA4FEBC6F63FDE0F8F4" box="[260,544,1808,1829]" italics="true" pageId="12" pageNumber="13">
|
||
<taxonomicName id="4C444D761B59FFA4FEBC6F63FE10F8F4" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[260,464,1808,1829]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="12" pageNumber="13" phylum="Rhodophyta" rank="species" species="eilatensis" status="sp. nov.">Gibsmithia eilatensis</taxonomicName>
|
||
<taxonomicNameLabel id="A203579C1B59FFA4FE6E6F63FDE0F8F4" box="[470,544,1808,1829]" pageId="12" pageNumber="13" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<caption id="DF3B667D1B58FFA5FF306ED6FEEBF7F3" ID-DOI="http://doi.org/10.5281/zenodo.13652702" ID-Zenodo-Dep="13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="13" pageNumber="14" startId="13.[136,229,1701,1722]" targetBox="[228,1359,190,1676]" targetPageId="13" targetType="figure">
|
||
<paragraph id="8BFB36F51B58FFA5FF306ED6FEEBF7F3" blockId="13.[136,1453,1701,2082]" pageId="13" pageNumber="14">
|
||
<emphasis id="B930EAE71B58FFA5FF306ED6FEC1F96B" bold="true" box="[136,257,1701,1722]" pageId="13" pageNumber="14">FIGURE 7.</emphasis>
|
||
<emphasis id="B930EAE71B58FFA5FEB26ED6FDEFF96B" box="[266,559,1701,1722]" italics="true" pageId="13" pageNumber="14">
|
||
<taxonomicName id="4C444D761B58FFA5FEB26ED6FE19F96B" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[266,473,1701,1722]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="13" pageNumber="14" phylum="Rhodophyta" rank="species" species="eilatensis" status="sp. nov.">Gibsmithia eilatensis</taxonomicName>
|
||
<taxonomicNameLabel id="A203579C1B58FFA5FE5A6ED6FDEFF96B" box="[482,559,1701,1722]" pageId="13" pageNumber="14" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<subSubSection id="C35E657E1B5BFFB8FF0568D3FA80FC58" lastPageId="16" lastPageNumber="17" pageId="14" pageNumber="15" type="description">
|
||
<paragraph id="8BFB36F51B5BFFA6FF0568D3FD64FCCC" blockId="14.[136,1452,160,2093]" pageId="14" pageNumber="15">DESCRIPTION: Thalli of furry gelatinous clusters of unbranched to pseudodichotomously branched blush, pink to white lobes, with unbranched cartilaginous stalk, and round, dark to light pink holdfast. Medullary filaments abundant, colorless, of elongate cells subtending assimilatory filaments comprised of main percurrent filaments and straight cortical filaments comprised of 20–35 rectilinear to semispherical cells, sparingly branched laterals, and thin rhizoidal filaments growing thallus inwards, intertwined with medullary filaments. Seirospore filaments present. Plants dioecious, with isomorphic tetrasporophytes. Carpogonial branches borne along the middle portion of cortical filaments, straight, 5–(8–10)–15 cells long, with the terminal 5–7 cells modified; basal cells of carpogonial branches occasionally bearing short unbranched vegetative laterals; conical carpogonium cut off by oblique division, offcentered on large hypogynous cell; subhypogynous cell smaller and rounder than the cells flanking it; trichogyne straight, elongate; hypogynous cell in functional carpogonial branches extend laterally, producing a bulge. Auxiliary cell branch 13–(19–20)–24 cells long, with 3–(5)–7 modified cells, rounder than surrounding cells, darkly staining; basal 3–10 cells with unbranched filaments surrounding carposporangia; 4–12 terminal cells rectilinear, decreasing in size towards apex. Connecting filaments septate, often diploidizing multiple auxiliary cells (>5) nearby. Gonimoblast initials cut off from bulge at junction of outgoing connecting filament with auxiliary cell. Spermatangia borne radially on 3–(5)–6 terminal cortical cells, forming corncob-like structures; spermatangial heads borne in a similar position of tetrasporangia. Tetrasporangia sessile, isolated, usually borne on the adaxial side of percurrent filaments, terminal or lateral on short lateral branches, in patches along the upper part of gelatinous branches, 10–20 cells below the surface; tetrasporangia decussately to cruciately divided.</paragraph>
|
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<paragraph id="8BFB36F51B5BFFA6FF056B5BFCFCFB20" blockId="14.[136,1452,160,2093]" pageId="14" pageNumber="15">
|
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<emphasis id="B930EAE71B5BFFA6FF056B5BFDF2FC90" box="[189,562,808,833]" italics="true" pageId="14" pageNumber="15">Habit and vegetative morphology.</emphasis>
|
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Thalli consist of furry, gelatinous clusters of unbranched to pseudodichotomously branched lobes (
|
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<figureCitation id="137F2A701B5BFFA6FE876B3FFE42FCB4" box="[319,386,844,869]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Fig. 6</figureCitation>
|
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) that are predominantly blush in color (
|
||
<figureCitation id="137F2A701B5BFFA6FC846B3FFC40FCB4" box="[828,896,844,869]" captionStart="FIGURE 4" captionStartId="11.[136,229,690,711]" captionTargetBox="[170,1423,262,656]" captionTargetId="figure-38@11.[151,1436,242,665]" captionTargetPageId="11" captionText="FIGURE 4. In vivo habits of G. eilatensis sp. nov. Specimens growing: A) attached to coral reef surface (DG219; isotype), B) between coral heads (DG210) and C) under a coral wall (DG234; holotype). Scale bar = 1.0 cm" figureDoi="http://doi.org/10.5281/zenodo.13652692" httpUri="https://zenodo.org/record/13652692/files/figure.png" pageId="14" pageNumber="15">Fig. 4</figureCitation>
|
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), occasionally pink to whitish (
|
||
<figureCitation id="137F2A701B5BFFA6FB656B3FFAF5FCB4" box="[1245,1333,844,869]" captionStart="FIGURE 1" captionStartId="1.[136,229,1922,1943]" captionTargetBox="[196,1391,190,1897]" captionTargetId="figure-14@1.[196,1391,190,1897]" captionTargetPageId="1" captionText="FIGURE 1. In situ habits of Gibsmithia hawaiiensis complex.A) DG234, Eilat, Israel. B) SGAD0509563, Kepulauan Seribu, Indonesia. C) SGAD0911460, Ternate, Indonesia. D) TIG001, Pulau Tiga, Malaysia. E) SGAD1205119, Johor, Malaysia. F) SGAD0712185, Raja Ampat, Indonesia. G) SGAD1206005, Labuan, Malaysia. Four individuals, each comprising one stipe and one to a few lobes. H) LEM03, Bitung, Indonesia. Scale bar 1 cm. Photo credits: A) T. Sauvage; B) A. Gittenberger; C, E–G) S.G.A. Draisma; D) M.A.S. Hussein; H) B.T. Reijnen." figureDoi="http://doi.org/10.5281/zenodo.13652682" httpUri="https://zenodo.org/record/13652682/files/figure.png" pageId="14" pageNumber="15">Fig. 1A</figureCitation>
|
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). Exserted cortical filaments from the lobes’ surface are prominently visible underwater (
|
||
<figureCitation id="137F2A701B5BFFA6FC496B03FB8AFC58" box="[1009,1098,880,905]" captionStart="FIGURE 1" captionStartId="1.[136,229,1922,1943]" captionTargetBox="[196,1391,190,1897]" captionTargetId="figure-14@1.[196,1391,190,1897]" captionTargetPageId="1" captionText="FIGURE 1. In situ habits of Gibsmithia hawaiiensis complex.A) DG234, Eilat, Israel. B) SGAD0509563, Kepulauan Seribu, Indonesia. C) SGAD0911460, Ternate, Indonesia. D) TIG001, Pulau Tiga, Malaysia. E) SGAD1205119, Johor, Malaysia. F) SGAD0712185, Raja Ampat, Indonesia. G) SGAD1206005, Labuan, Malaysia. Four individuals, each comprising one stipe and one to a few lobes. H) LEM03, Bitung, Indonesia. Scale bar 1 cm. Photo credits: A) T. Sauvage; B) A. Gittenberger; C, E–G) S.G.A. Draisma; D) M.A.S. Hussein; H) B.T. Reijnen." figureDoi="http://doi.org/10.5281/zenodo.13652682" httpUri="https://zenodo.org/record/13652682/files/figure.png" pageId="14" pageNumber="15">Fig. 1A</figureCitation>
|
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). The unbranched cartilaginous stalk (
|
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<figureCitation id="137F2A701B5BFFA6FF756BE7FED7FC7C" box="[205,279,916,941]" captionStart="FIGURE 4" captionStartId="11.[136,229,690,711]" captionTargetBox="[170,1423,262,656]" captionTargetId="figure-38@11.[151,1436,242,665]" captionTargetPageId="11" captionText="FIGURE 4. In vivo habits of G. eilatensis sp. nov. Specimens growing: A) attached to coral reef surface (DG219; isotype), B) between coral heads (DG210) and C) under a coral wall (DG234; holotype). Scale bar = 1.0 cm" figureDoi="http://doi.org/10.5281/zenodo.13652692" httpUri="https://zenodo.org/record/13652692/files/figure.png" pageId="14" pageNumber="15">Figs 4</figureCitation>
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,
|
||
<figureCitation id="137F2A701B5BFFA6FE9C6BE7FE88FC7C" box="[292,328,916,941]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">6A</figureCitation>
|
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) is attached to the substratum by a round, dark to light pink holdfast. Cortical filaments are cut off alternately and sparingly from widely divergent main assimilatory filaments referred to as percurrent filaments and consist of rectilinear to spherical cells decreasing in size towards the surface (5–166.25) × (3.75–11.25) μm (
|
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<figureCitation id="137F2A701B5BFFA6FAC26BAFFF0EFBC8" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Figs 6B–C</figureCitation>
|
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). Abundant colorless medullary cells are elongated and transition into distal assimilatory percurrent filaments (
|
||
<figureCitation id="137F2A701B5BFFA6FF286C57FF27FBEC" box="[144,231,1060,1085]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Fig. 6C</figureCitation>
|
||
) whose cells measure 36.25–300 × 3.75–11.25 μm. Cells of lower cortical filaments cut off narrow rhizoidal filaments that connect to nearby rhizoidal cells (
|
||
<figureCitation id="137F2A701B5BFFA6FD0A6C3BFCCDFBB0" box="[690,781,1096,1121]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Fig. 6B</figureCitation>
|
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). Hair-like structures (6D) of variable length (12.5–120) × 1.25 μm occasionally extend from the apical cells of cortical filaments and staining darkly with aniline blue when short and become almost colorless when long. Rhizoidal filaments cut off from lower cortical cells grow thallus inward where they become intertwined with medullary filaments. Straight, small-celled, scarcely branched and unbranched seirospore filaments are present (
|
||
<figureCitation id="137F2A701B5BFFA6FE436CABFD90FB20" box="[507,592,1240,1265]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Fig. 6E</figureCitation>
|
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) in tetrasporophytes.
|
||
</paragraph>
|
||
<paragraph id="8BFB36F51B5BFFA7FF056C8FFDEAFDF0" blockId="14.[136,1452,160,2093]" lastBlockId="15.[136,1452,160,545]" lastPageId="15" lastPageNumber="16" pageId="14" pageNumber="15">
|
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<emphasis id="B930EAE71B5BFFA6FF056C8FFE26FAC4" box="[189,486,1276,1301]" italics="true" pageId="14" pageNumber="15">Reproductive morphology.</emphasis>
|
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Thalli are dioecious, with isomorphic tetrasporophytes. Female pre-fertilization stages are scattered among elongated, narrow assimilatory filaments (
|
||
<figureCitation id="137F2A701B5BFFA6FCFE6D53FC5EFAE8" box="[838,926,1312,1337]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Fig. 6C</figureCitation>
|
||
). Carpogonial branches are formed laterally on assimilatory filaments, replacing normal vegetative filaments (
|
||
<figureCitation id="137F2A701B5BFFA6FCFB6D37FC7DFA8C" box="[835,957,1348,1373]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Figs 6F–G</figureCitation>
|
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) in which the 5–9 upper cells transform into spherical cells while the subtending lower cells remain narrow and elliptic like other vegetative cells (
|
||
<figureCitation id="137F2A701B5BFFA6FAAB6D1BFA60FA50" box="[1299,1440,1384,1409]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Figs 6F–6G</figureCitation>
|
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). Transformed cells of the carpogonial filaments remain unbranched while the basal and lowermost untransformed cells may cut off short unbranched lateral cell strands growing distally and obliquely (
|
||
<figureCitation id="137F2A701B5BFFA6FBD96DC3FB23FA18" box="[1121,1251,1456,1481]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Figs 7A–B</figureCitation>
|
||
). The unfertilized carpogonium is conical with a long, straight trichogyne (
|
||
<figureCitation id="137F2A701B5BFFA6FD466DA7FCB9FA3C" box="[766,889,1492,1517]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Figs 6F–G</figureCitation>
|
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,
|
||
<figureCitation id="137F2A701B5BFFA6FC3C6DA7FC0AFA3C" box="[900,970,1492,1517]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">7A–B</figureCitation>
|
||
), separated by an oblique division from the hypogynous cell. The hypogynous cell extends laterally and obliquely (
|
||
<figureCitation id="137F2A701B5BFFA6FC116D8BFBE7F9C0" box="[937,1063,1528,1553]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Figs 6F–G</figureCitation>
|
||
). The subelliptical subhypogynous cell is very small (
|
||
<figureCitation id="137F2A701B5BFFA6FEED6E6FFE10F9E4" box="[341,464,1564,1589]" captionStart="FIGURE 6" captionStartId="12.[136,229,1808,1829]" captionTargetBox="[196,1391,190,1782]" captionTargetId="figure-15@12.[196,1391,190,1784]" captionTargetPageId="12" captionText="FIGURE 6. Gibsmithia eilatensis sp. nov. A) Holotype, female gametophyte, DG234, Eilat, Israel. B) Assimilatory filaments comprised of main percurrent filaments (arrow), cortical filaments (arrowhead) and rhizoidal filaments (double arrowhead). Rhizoidal filaments with cells connected to neighboring rhizoidal cells. Slide DG219-2. Scale bar 200 μm. C) Female pre-fertilization stages among elongated, narrow assimilatory filaments. Slide DG234-3. Scale bar 200 μm. D) Hairlike extensions from apical cortical cells. Slide DG210-2. Scale bar 50 μm. E) Branched and unbranched “seirospore” filaments. Slide DG210-5. Scale bar 50 μm. F) Carpogonial branch laterally positioned on assimilatory filaments, with straight and obliquely (arrow) directed trichogynes. Slide DG210-1. Scale bar 50 μm. G) Carpogonial branch with laterally extended hypogynous cell (double arrowhead), small subhypogynous cell (arrowhead) and conical carpogonium (arrow) with straight trichogyne. Slide DG210-4. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652698" httpUri="https://zenodo.org/record/13652698/files/figure.png" pageId="14" pageNumber="15">Figs 6F–G</figureCitation>
|
||
,
|
||
<figureCitation id="137F2A701B5BFFA6FE636E6FFDC0F9E4" box="[475,512,1564,1589]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">7A</figureCitation>
|
||
), with the cell subtending it the largest cell of the carpogonial branch. Auxiliary cell filaments (
|
||
<figureCitation id="137F2A701B5BFFA6FEB96E33FE99F988" box="[257,345,1600,1625]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Fig. 7C</figureCitation>
|
||
) are homologous in position and origin to the carpogonial branches and cortical filaments. Three to seven transformed intercalary cells of an auxiliary cell filaments (
|
||
<figureCitation id="137F2A701B5BFFA6FC196E17FBDEF9AC" box="[929,1054,1636,1661]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Figs 7C–E</figureCitation>
|
||
) become darkly staining, enlarged and roundish, and flank below and above an auxiliary cell that remains small, cytoplasm-poor and colorless (
|
||
<figureCitation id="137F2A701B5BFFA6FAC26EFBFF0CF914" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Figs 7D–F</figureCitation>
|
||
). Some of these dark cells or untransformed cells below bear unbranched laterals growing distally and obliquely (
|
||
<figureCitation id="137F2A701B5BFFA6FF286EA3FF2AF938" box="[144,234,1744,1769]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Fig. 7C</figureCitation>
|
||
). After presumed fertilization, the carpogonium produces a lateral extension that follows the orientation of the expanding hypogynous cell (
|
||
<figureCitation id="137F2A701B5BFFA6FE466E87FD4EF8DC" box="[510,654,1780,1805]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Figs 7A–7B</figureCitation>
|
||
). Upon reaching an auxiliary cell, an unsegmented, hyaline incoming connecting filament connects laterally to the upper side of the auxiliary cell (
|
||
<figureCitation id="137F2A701B5BFFA6FC4B6F6BFB8EF8E0" box="[1011,1102,1816,1841]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Fig. 7D</figureCitation>
|
||
). It is assumed that during this process a product of the fertilization nucleus is transferred from the connecting filament to the auxiliary cell. Following this diploidization of an auxiliary cell, the incoming connecting filament continues to grow as an outgoing connecting filament (
|
||
<figureCitation id="137F2A701B5BFFA6FF4B6FF7FE8BF84C" box="[243,331,1924,1949]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Fig. 7D</figureCitation>
|
||
). When reaching another auxiliary cell, the former outgoing filament acts as a new incoming filament, developing cross walls at the site of a new diploidization (
|
||
<figureCitation id="137F2A701B5BFFA6FCAF6FDBFCADF810" box="[791,877,1960,1985]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Fig. 7E</figureCitation>
|
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) that leads to the formation of a short segment that partly fuses with the auxiliary cell and then continues as an outgoing connecting filament (
|
||
<figureCitation id="137F2A701B5BFFA6FBC56FBFFB14F834" box="[1149,1236,1996,2021]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Fig. 7E</figureCitation>
|
||
). A single roundish gonimoblast initial (
|
||
<figureCitation id="137F2A701B5BFFA6FED16F83FE20F7D8" box="[361,480,2032,2057]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="14" pageNumber="15">Figs 7E–F</figureCitation>
|
||
) is cut off from the short segment formed on the point of contact between the auxiliary cell and the connecting filament. The outgoing connecting filament continues to reach nearby auxiliary cells and before reaching a new auxiliary cell develops a cross wall, forms a short segment that contains part of the divided fertilization nucleus which fuses with the auxiliary cell (
|
||
<figureCitation id="137F2A701B5AFFA7FDC468B7FD37FF0C" box="[636,759,196,221]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="15" pageNumber="16">Figs 7E–F</figureCitation>
|
||
). Following the formation of the first gonimoblast initial, the fertilization nucleus in the generative segment cuts off additional gonimoblast cells that each will continue to divide laterally and radially resulting in small clusters of cells (
|
||
<figureCitation id="137F2A701B5AFFA7FD4F697FFCB8FEF4" box="[759,888,268,293]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="15" pageNumber="16">Figs 7G–H</figureCitation>
|
||
). The issuing of a connecting filament primordium from the carpogonium was only observed once (
|
||
<figureCitation id="137F2A701B5AFFA7FD1E6943FD3DFE98" box="[678,765,304,329]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="15" pageNumber="16">Fig. 7B</figureCitation>
|
||
), prior to its fusion with the hypogynous cell and cells below the subhypogynous cell. Mature auxiliary cell branches are 13–(19–20)–24 cells long (
|
||
<figureCitation id="137F2A701B5AFFA7FBD26927FB2CFEBC" box="[1130,1260,340,365]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="15" pageNumber="16">Figs 7C–H</figureCitation>
|
||
). The lowermost 3–10 cells of the auxiliary cell filament typically cut off short-celled unbranched vegetative filaments that surround the gonimoblast cells (7G–H) as they mature into carposporangia. The upper 4–12 terminal cells of the auxiliary cell filament are rectilinear, decreasing in size toward the apex (
|
||
<figureCitation id="137F2A701B5AFFA7FCF869B3FBC3FE08" box="[832,1027,448,473]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="15" pageNumber="16">Figs 7C, 7E, 7H</figureCitation>
|
||
). Gonimoblast cells resulting from a single diploidization event mature gradually in a synchronized fashion, resulting in same-sized cells (
|
||
<figureCitation id="137F2A701B5AFFA7FB566997FA88FE2C" box="[1262,1352,484,509]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="15" pageNumber="16">Fig. 7H</figureCitation>
|
||
). Mature carposporophytes were not observed.
|
||
</paragraph>
|
||
<caption id="DF3B667D1B5AFFA7FF306F63FDF5F840" ID-DOI="http://doi.org/10.5281/zenodo.13652706" ID-Zenodo-Dep="13652706" httpUri="https://zenodo.org/record/13652706/files/figure.png" pageId="15" pageNumber="16" startId="15.[136,229,1808,1829]" targetBox="[153,1433,572,1779]" targetPageId="15" targetType="figure">
|
||
<paragraph id="8BFB36F51B5AFFA7FF306F63FDF5F840" blockId="15.[136,1452,1808,1937]" pageId="15" pageNumber="16">
|
||
<emphasis id="B930EAE71B5AFFA7FF306F63FEC0F8F4" bold="true" box="[136,256,1808,1829]" pageId="15" pageNumber="16">FIGURE 8.</emphasis>
|
||
<emphasis id="B930EAE71B5AFFA7FEB16F63FDEBF8F4" box="[265,555,1808,1829]" italics="true" pageId="15" pageNumber="16">
|
||
<taxonomicName id="4C444D761B5AFFA7FEB16F63FE17F8F4" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[265,471,1808,1829]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="15" pageNumber="16" phylum="Rhodophyta" rank="species" species="eilatensis" status="sp. nov.">Gibsmithia eilatensis</taxonomicName>
|
||
<taxonomicNameLabel id="A203579C1B5AFFA7FE676F63FDEBF8F4" box="[479,555,1808,1829]" pageId="15" pageNumber="16" rank="species">sp. nov.</taxonomicNameLabel>
|
||
</emphasis>
|
||
A–B) Mature corncob-like spermatangial heads. A) Slide DG220-1. Scale bar 50 μm. B) Slide DG220-1, scale bar 20 μm. C) Young and mature tetrasporangia. Slide DG219-1. Scale bar 20 μm. D–E) Terminal and lateral tetrasporangial initials growing on lateral assimilatory filaments, and decussate and cruciate tetrasporangia. D) Slide DG219-1. Scale bar 50 μm. E) Slide DG219-1. Scale bar 20 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8BFB36F51B5AFFB8FF056FB2FBB6FEF4" blockId="15.[136,1452,1985,2082]" lastBlockId="16.[136,1452,160,905]" lastPageId="16" lastPageNumber="17" pageId="15" pageNumber="16">
|
||
Young clusters of spermatangia are formed on terminal and lateral cells of assimilatory cortical filaments on dioecious gametophytes (
|
||
<figureCitation id="137F2A701B5AFFA7FE156F96FDC0F82F" box="[429,512,2021,2046]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="15" pageNumber="16">Fig. 7I</figureCitation>
|
||
). The spermatangial initials are cut off radially as multiple small protrusions on 3–(5)–6 consecutive cells in a single filament. When the lateral cell filament is 7 cells long, the 6–celled spermatangial branch is pedicellate (
|
||
<figureCitation id="137F2A701B45FFB8FE3968D3FDC2FF68" box="[385,514,160,185]" captionStart="FIGURE 8" captionStartId="15.[136,229,1808,1829]" captionTargetBox="[153,1433,572,1779]" captionTargetId="figure-194@15.[151,1436,569,1782]" captionTargetPageId="15" captionText="FIGURE 8. Gibsmithia eilatensis sp. nov. A–B) Mature corncob-like spermatangial heads. A) Slide DG220-1. Scale bar 50 μm. B) Slide DG220-1, scale bar 20 μm. C) Young and mature tetrasporangia. Slide DG219-1. Scale bar 20 μm. D–E) Terminal and lateral tetrasporangial initials growing on lateral assimilatory filaments, and decussate and cruciate tetrasporangia. D) Slide DG219-1. Scale bar 50 μm. E) Slide DG219-1. Scale bar 20 μm." figureDoi="http://doi.org/10.5281/zenodo.13652706" httpUri="https://zenodo.org/record/13652706/files/figure.png" pageId="16" pageNumber="17">Figs 8A–B</figureCitation>
|
||
); if 6 cells long the corncob-like structure is sessile, and if more than 6 cells long, the male reproductive structure is terminally positioned on a lateral branch (
|
||
<figureCitation id="137F2A701B45FFB8FC4268B7FB8CFF0C" box="[1018,1100,196,221]" captionStart="FIGURE 7" captionStartId="13.[136,229,1701,1722]" captionTargetBox="[228,1359,190,1676]" captionTargetId="figure-14@13.[228,1359,190,1676]" captionTargetPageId="13" captionText="FIGURE 7. Gibsmithia eilatensis sp. nov. Early post-fertilization stages. A) Vegetative lateral branch (arrowhead) on basal cell of carpogonial filament, and curved hypogynous cell (arrow). Slide DG210-4. Scale bar 50 μm. B) Presumed early fertilization stage showing lateral extension of carpogonium and hypogynous cell (arrowhead). Slide DG210-1. Scale bar 50 μm. C) Auxiliary cell branch. Slide DG234-4. Scale bar 50 μm. D) Unsegmented incoming connecting filament (ic) partly and laterally fused to auxiliary cell, and unseptated outgoing connecting filament (oc). Slide DG234-2. Scale bar 100 μm. E) Incoming connecting filament (ic) segment partly fused with auxiliary cell, and outgoing connecting filament (oc) segment (arrow) partly fused with nearby auxiliary cell. Slide DG234- 3. Scale bar 50 μm. F) Gonimoblast initial (arrow) developed from the short segment partly fused to auxiliary cell, between incoming connecting filament (ic) and outgoing connecting filament (oc). Slide DG234-3. Scale bar 50 μm. G–H) Multiple gonimoblast initials cut off bilaterally from connecting filament segment (arrow) fused to auxiliary cell. G) Slide DG234-3. Scale bar 50 μm. H) Slide DG234-5. Scale bar 50 μm. I) Young clusters of spermatangia (arrow) formed terminally and laterally in assimilatory filaments. Slide DG220-1. Scale bar 50 μm." figureDoi="http://doi.org/10.5281/zenodo.13652702" httpUri="https://zenodo.org/record/13652702/files/figure.png" pageId="16" pageNumber="17">Fig. 7I</figureCitation>
|
||
). Mature spermatangial heads, 25–(50–81.25) × 11.25(15–18.75) μm (
|
||
<figureCitation id="137F2A701B45FFB8FD86689BFD7DFED0" box="[574,701,232,257]" captionStart="FIGURE 8" captionStartId="15.[136,229,1808,1829]" captionTargetBox="[153,1433,572,1779]" captionTargetId="figure-194@15.[151,1436,569,1782]" captionTargetPageId="15" captionText="FIGURE 8. Gibsmithia eilatensis sp. nov. A–B) Mature corncob-like spermatangial heads. A) Slide DG220-1. Scale bar 50 μm. B) Slide DG220-1, scale bar 20 μm. C) Young and mature tetrasporangia. Slide DG219-1. Scale bar 20 μm. D–E) Terminal and lateral tetrasporangial initials growing on lateral assimilatory filaments, and decussate and cruciate tetrasporangia. D) Slide DG219-1. Scale bar 50 μm. E) Slide DG219-1. Scale bar 20 μm." figureDoi="http://doi.org/10.5281/zenodo.13652706" httpUri="https://zenodo.org/record/13652706/files/figure.png" pageId="16" pageNumber="17">Figs 8A–B</figureCitation>
|
||
), are oblong and borne in a similar position as the tetrasporangia on cortical filaments. Spermatangia are abundant, measuring (2–2.5) × (1.5–2) μm (
|
||
<figureCitation id="137F2A701B45FFB8FBA9697FFBA9FEF4" box="[1041,1129,268,293]" captionStart="FIGURE 8" captionStartId="15.[136,229,1808,1829]" captionTargetBox="[153,1433,572,1779]" captionTargetId="figure-194@15.[151,1436,569,1782]" captionTargetPageId="15" captionText="FIGURE 8. Gibsmithia eilatensis sp. nov. A–B) Mature corncob-like spermatangial heads. A) Slide DG220-1. Scale bar 50 μm. B) Slide DG220-1, scale bar 20 μm. C) Young and mature tetrasporangia. Slide DG219-1. Scale bar 20 μm. D–E) Terminal and lateral tetrasporangial initials growing on lateral assimilatory filaments, and decussate and cruciate tetrasporangia. D) Slide DG219-1. Scale bar 50 μm. E) Slide DG219-1. Scale bar 20 μm." figureDoi="http://doi.org/10.5281/zenodo.13652706" httpUri="https://zenodo.org/record/13652706/files/figure.png" pageId="16" pageNumber="17">Fig. 8B</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8BFB36F51B45FFB8FF056943FEC5FE64" blockId="16.[136,1452,160,905]" pageId="16" pageNumber="17">
|
||
Tetrasporangial initials (
|
||
<figureCitation id="137F2A701B45FFB8FE756943FD8AFE98" box="[461,586,304,329]" captionStart="FIGURE 8" captionStartId="15.[136,229,1808,1829]" captionTargetBox="[153,1433,572,1779]" captionTargetId="figure-194@15.[151,1436,569,1782]" captionTargetPageId="15" captionText="FIGURE 8. Gibsmithia eilatensis sp. nov. A–B) Mature corncob-like spermatangial heads. A) Slide DG220-1. Scale bar 50 μm. B) Slide DG220-1, scale bar 20 μm. C) Young and mature tetrasporangia. Slide DG219-1. Scale bar 20 μm. D–E) Terminal and lateral tetrasporangial initials growing on lateral assimilatory filaments, and decussate and cruciate tetrasporangia. D) Slide DG219-1. Scale bar 50 μm. E) Slide DG219-1. Scale bar 20 μm." figureDoi="http://doi.org/10.5281/zenodo.13652706" httpUri="https://zenodo.org/record/13652706/files/figure.png" pageId="16" pageNumber="17">Figs 8C–D</figureCitation>
|
||
) are cut off unilaterally in short series either obliquely from the upper side of cells on short laterals on cortical filaments, or are terminal on these short laterals in the upper part of the gelatinous branches. Tetrasporangia are usually decussately divided (
|
||
<figureCitation id="137F2A701B45FFB8FCB2690BFC9FFE40" box="[778,863,376,401]" captionStart="FIGURE 8" captionStartId="15.[136,229,1808,1829]" captionTargetBox="[153,1433,572,1779]" captionTargetId="figure-194@15.[151,1436,569,1782]" captionTargetPageId="15" captionText="FIGURE 8. Gibsmithia eilatensis sp. nov. A–B) Mature corncob-like spermatangial heads. A) Slide DG220-1. Scale bar 50 μm. B) Slide DG220-1, scale bar 20 μm. C) Young and mature tetrasporangia. Slide DG219-1. Scale bar 20 μm. D–E) Terminal and lateral tetrasporangial initials growing on lateral assimilatory filaments, and decussate and cruciate tetrasporangia. D) Slide DG219-1. Scale bar 50 μm. E) Slide DG219-1. Scale bar 20 μm." figureDoi="http://doi.org/10.5281/zenodo.13652706" httpUri="https://zenodo.org/record/13652706/files/figure.png" pageId="16" pageNumber="17">Fig. 8E</figureCitation>
|
||
), sometimes cruciate (
|
||
<figureCitation id="137F2A701B45FFB8FBEE690BFB6CFE40" box="[1110,1196,376,401]" captionStart="FIGURE 8" captionStartId="15.[136,229,1808,1829]" captionTargetBox="[153,1433,572,1779]" captionTargetId="figure-194@15.[151,1436,569,1782]" captionTargetPageId="15" captionText="FIGURE 8. Gibsmithia eilatensis sp. nov. A–B) Mature corncob-like spermatangial heads. A) Slide DG220-1. Scale bar 50 μm. B) Slide DG220-1, scale bar 20 μm. C) Young and mature tetrasporangia. Slide DG219-1. Scale bar 20 μm. D–E) Terminal and lateral tetrasporangial initials growing on lateral assimilatory filaments, and decussate and cruciate tetrasporangia. D) Slide DG219-1. Scale bar 50 μm. E) Slide DG219-1. Scale bar 20 μm." figureDoi="http://doi.org/10.5281/zenodo.13652706" httpUri="https://zenodo.org/record/13652706/files/figure.png" pageId="16" pageNumber="17">Fig. 8C</figureCitation>
|
||
), (15–18.75) × (11.25– 13.75) μm.
|
||
</paragraph>
|
||
<paragraph id="8BFB36F51B45FFB8FF0569B3FF21FCB4" blockId="16.[136,1452,160,905]" pageId="16" pageNumber="17">
|
||
NOTE: The present description was based on a small number of samples found after extensive searches along the coast of Eilat. Although all three life history phases were observed for
|
||
<taxonomicName id="4C444D761B45FFB8FC2B6997FBDEFE2C" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[915,1054,484,509]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="16" pageNumber="17" phylum="Rhodophyta" rank="species" species="eilatensis">
|
||
<emphasis id="B930EAE71B45FFB8FC2B6997FBDEFE2C" box="[915,1054,484,509]" italics="true" pageId="16" pageNumber="17">G. eilatensis</emphasis>
|
||
</taxonomicName>
|
||
, connecting filament formation and mature cystocarps were not observed in our extensive squash preparations. Unlike those of
|
||
<taxonomicName id="4C444D761B45FFB8FB276A7BFA87FDF0" authorityName="Doty" authorityYear="1963" box="[1183,1351,520,545]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="16" pageNumber="17" phylum="Rhodophyta" rank="species" species="hawaiiensis">
|
||
<emphasis id="B930EAE71B45FFB8FB276A7BFA87FDF0" box="[1183,1351,520,545]" italics="true" pageId="16" pageNumber="17">G. hawaiiensis</emphasis>
|
||
</taxonomicName>
|
||
, cortical filaments in
|
||
<taxonomicName id="4C444D761B45FFB8FEAD6A5FFE61FD94" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[277,417,556,581]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="16" pageNumber="17" phylum="Rhodophyta" rank="species" species="eilatensis">
|
||
<emphasis id="B930EAE71B45FFB8FEAD6A5FFE61FD94" box="[277,417,556,581]" italics="true" pageId="16" pageNumber="17">G. eilatensis</emphasis>
|
||
</taxonomicName>
|
||
are rarely cut off oppositely from the main percurrent filaments and are usually unbranched or sparingly alternately branched (
|
||
<tableCitation id="C6C6034E1B45FFB8FDAB6A23FDADFDB8" box="[531,621,592,617]" captionStart="TABLE 2" captionStartId="16.[136,216,954,975]" captionText="TABLE 2. Anatomical comparison between Gibsmithia eilatensis sp. nov. and G. hawaiiensis Doty 1963. Based on observations from Doty (1963) and the present study." pageId="16" pageNumber="17">Table 2</tableCitation>
|
||
). Seirospores are found in chains as in
|
||
<taxonomicName id="4C444D761B45FFB8FB806A23FB21FDB8" authorityName="Doty" authorityYear="1963" box="[1080,1249,592,617]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="16" pageNumber="17" phylum="Rhodophyta" rank="species" species="hawaiiensis">
|
||
<emphasis id="B930EAE71B45FFB8FB806A23FB21FDB8" box="[1080,1249,592,617]" italics="true" pageId="16" pageNumber="17">G. hawaiiensis</emphasis>
|
||
</taxonomicName>
|
||
, but occasionally present short branchlets. Carpogonial branches bear an enlarged hypogynous cell and a very small subhypogynous cell, while all cells of the carpogonial branch are similar in size and shape in
|
||
<taxonomicName id="4C444D761B45FFB8FC7B6AEBFBABFD60" authorityName="Doty" authorityYear="1963" box="[963,1131,664,689]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="16" pageNumber="17" phylum="Rhodophyta" rank="species" species="hawaiiensis">
|
||
<emphasis id="B930EAE71B45FFB8FC7B6AEBFBABFD60" box="[963,1131,664,689]" italics="true" pageId="16" pageNumber="17">G. hawaiiensis</emphasis>
|
||
</taxonomicName>
|
||
. Auxiliary cell branches are also composed of similarly shaped modified cells in
|
||
<taxonomicName id="4C444D761B45FFB8FD756ACFFCB2FD04" authorityName="Doty" authorityYear="1963" box="[717,882,700,725]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="16" pageNumber="17" phylum="Rhodophyta" rank="species" species="hawaiiensis">
|
||
<emphasis id="B930EAE71B45FFB8FD756ACFFCB2FD04" box="[717,882,700,725]" italics="true" pageId="16" pageNumber="17">G. hawaiiensis</emphasis>
|
||
</taxonomicName>
|
||
, while auxiliary cell is smaller in
|
||
<taxonomicName id="4C444D761B45FFB8FB506ACFFAB4FD04" authorityName="Gabriel & Draisma & Sauvage & Schmidt & Schils & Lim & Harris & Fredericq" authorityYear="2016" box="[1256,1396,700,725]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="16" pageNumber="17" phylum="Rhodophyta" rank="species" species="eilatensis">
|
||
<emphasis id="B930EAE71B45FFB8FB506ACFFAB4FD04" box="[1256,1396,700,725]" italics="true" pageId="16" pageNumber="17">G. eilatensis</emphasis>
|
||
</taxonomicName>
|
||
. The origin of the gonimoblast initials is from the point of junction between the connecting filament and the auxiliary cell, contrasting with the generitype, where gonimoblasts are initiated from the auxiliary cell. Tetrasporangia are mostly borne on short lateral branches on inner cells of cortical filaments, while in
|
||
<taxonomicName id="4C444D761B45FFB8FC4E6B5BFB5EFC90" authorityName="Doty" authorityYear="1963" box="[1014,1182,808,833]" family="Dumontiaceae" genus="Gibsmithia" kingdom="Plantae" order="Cryptonemiales" pageId="16" pageNumber="17" phylum="Rhodophyta" rank="species" species="hawaiiensis">
|
||
<emphasis id="B930EAE71B45FFB8FC4E6B5BFB5EFC90" box="[1014,1182,808,833]" italics="true" pageId="16" pageNumber="17">G. hawaiiensis</emphasis>
|
||
</taxonomicName>
|
||
they have a 2–3-celled pedicel.
|
||
</paragraph>
|
||
<paragraph id="8BFB36F51B45FFB8FF056B03FA80FC58" blockId="16.[136,1452,160,905]" box="[189,1344,880,905]" pageId="16" pageNumber="17">
|
||
REPRESENTATIVE SEQUENCES: KM406844 (COI-5P), KM392352 (
|
||
<emphasis id="B930EAE71B45FFB8FC4A6B03FBD7FC58" box="[1010,1047,880,905]" italics="true" pageId="16" pageNumber="17">rbc</emphasis>
|
||
L) and KM392317 (UPA).
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |