244 lines
31 KiB
XML
244 lines
31 KiB
XML
<document id="BD3A2919E2158F37E5613BEA3E9D3EF0" ID-DOI="10.11646/phytotaxa.64.1.3" ID-ISSN="1179-3163" ID-Zenodo-Dep="5062002" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1625237296819" checkinUser="felipe" docAuthor="Novis, Phil M., Braidwood, Jasmine & Kilroy, Cathy" docDate="2012" docId="039987EEFFA52F16FF015301FDE4FCE3" docLanguage="en" docName="Phytotaxa.64.11-45.pdf" docOrigin="Phytotaxa 64 (1)" docSource="http://biotaxa.org/Phytotaxa/article/view/phytotaxa.64.1.3" docStyle="DocumentStyle:13F189B31CB4CCCE9197361A4E100104.3:Phytotaxa.2011-2013.journal_article" docStyleId="13F189B31CB4CCCE9197361A4E100104" docStyleName="Phytotaxa.2011-2013.journal_article" docStyleVersion="3" docTitle="Nitzschia palea Smith 1856" docType="treatment" docVersion="3" lastPageNumber="26" masterDocId="FFA0FF96FFA82F19FF96555FFFF4FFA0" masterDocTitle="Small diatoms (Bacillariophyta) in cultures from the Styx River, New Zealand, including descriptions of three new species" masterLastPageNumber="45" masterPageNumber="11" pageNumber="24" updateTime="1698984801949" updateUser="plazi" zenodo-license-document="CLOSED">
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<mods:title id="677BD4DBFC5599F5F047972ECD9BCB0D">Small diatoms (Bacillariophyta) in cultures from the Styx River, New Zealand, including descriptions of three new species</mods:title>
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<mods:namePart id="0F1B971C7180EE03B9407BB422C77052">Novis, Phil M.</mods:namePart>
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<mods:affiliation id="452039F88F3387BA03933EF4BCA98CD5">Allan Herbarium, Landcare Research, P. O. Box 40, Lincoln 7640, New Zealand</mods:affiliation>
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<mods:namePart id="CC405B655A28F8E21DBCC50F72449754">Braidwood, Jasmine</mods:namePart>
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<mods:affiliation id="62CBA9D4D54A31659A48A92AD97997F8">Allan Herbarium, Landcare Research, P. O. Box 40, Lincoln 7640, New Zealand</mods:affiliation>
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<mods:namePart id="9429E4778F7D402614D89E8DDBEB80D7">Kilroy, Cathy</mods:namePart>
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<mods:affiliation id="2EEFC1AAA973324739CFC56C281FF065">National Institute of Water and Atmospheric Research, P. O. Box 8602, Christchurch, New Zealand</mods:affiliation>
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<mods:title id="46458C5EA290D6C28B284B3A749A7FF8">Phytotaxa</mods:title>
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<mods:date id="BA7FF04DB9C964A9A55ED067AE07861B">2012</mods:date>
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<mods:number id="E6545E1C50C66D4A052279D5C33824DD">2012-08-24</mods:number>
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<subSubSection id="C32A6573FFA52F14FF015301FD8EF8E2" pageId="13" pageNumber="24" type="nomenclature">
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<paragraph id="8B8F36F8FFA52F14FF015301FCADF9DB" blockId="13.[151,857,1630,1659]" box="[151,857,1630,1659]" pageId="13" pageNumber="24">
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<heading id="D0C78194FFA52F14FF015301FCADF9DB" bold="true" box="[151,857,1630,1659]" fontSize="12" level="1" pageId="13" pageNumber="24" reason="1">
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<emphasis id="B944EAEAFFA52F14FF015301FCADF9DB" bold="true" box="[151,857,1630,1659]" pageId="13" pageNumber="24">
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<taxonomicName id="4C304D7BFFA52F14FF015301FD9FF9DB" authority="(Kutz.) Smith 1856" authorityName="Smith" authorityYear="1856" baseAuthorityName="Kutz." box="[151,619,1630,1659]" class="Monogenea" family="Capsalidae" genus="Nitzschia" kingdom="Animalia" order="Capsalidea" pageId="13" pageNumber="24" phylum="Platyhelminthes" rank="species" species="palea">
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<emphasis id="B944EAEAFFA52F14FF015301FE94F9DA" bold="true" box="[151,352,1630,1658]" italics="true" pageId="13" pageNumber="24">Nitzschia palea</emphasis>
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(Kütz.)
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<bibRefCitation id="EFA14B09FFA52F14FE475301FD9FF9DB" author="Smith, W." box="[465,619,1630,1659]" pageId="13" pageNumber="24" refId="ref20254" refString="Smith, W. (1856) A synopsis of the British Diatomaceae; with remarks on their structure, functions and distribution; and instructions for collecting and preserving specimens. Vol. 2. John van Voorst, London, 107 pp." type="book" year="1856">Smith 1856</bibRefCitation>
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</taxonomicName>
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(
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<figureCitation id="130B2A7DFFA52F14FDEA5301FCE4F9DB" box="[636,784,1630,1659]" captionStart="FIGURE 52–64" captionStartId="14.[151,244,1803,1824]" captionTargetBox="[151,1436,887,1782]" captionTargetId="figure-397@14.[151,1436,887,1782]" captionTargetPageId="14" captionText="FIGURE 52–64. Nitzschia palea, strain LCR-S:6:4:1, CHR618410. Scales: Fig. 52, 10 µm; Fig. 61, 5 µm (use for Figs 53–62); Fig. 63, 0.5 µm; Fig. 64, 1 µm. Fig. 52. Live cells viewed by LM. Figs 53–62. Cleaned frustules viewed by LM, showing size variation and raphe canals on opposite margins (Figs 53, 54 are single valves; raphe is visible in Fig. 53). Fig. 63. TEM of cleaned frustule, centre of valve, showing continuous raphe with no central termini." figureDoi="http://doi.org/10.5281/zenodo.5062016" httpUri="https://zenodo.org/record/5062016/files/figure.png" pageId="13" pageNumber="24">Figs 52–64</figureCitation>
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,
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<figureCitation id="130B2A7DFFA52F14FC885301FCA4F9DB" box="[798,848,1630,1659]" captionStart="FIGURE 162" captionStartId="28.[151,244,1590,1611]" captionTargetBox="[154,1433,626,1529]" captionTargetId="figure-383@28.[151,1436,617,1569]" captionTargetPageId="28" captionText="FIGURE 162. Bayesian phylogenies of Nitzschia strains from the Styx River, for rbcL (left) and 18S rDNA (right). Support numbers above branches are Bayesian posterior probabilities / Maximum Parsimony bootstrap values. Scale bar = 0.1 subsitutions/site. See text for substitution models and description of data sets." figureDoi="http://doi.org/10.5281/zenodo.5062040" httpUri="https://zenodo.org/record/5062040/files/figure.png" pageId="13" pageNumber="24">162</figureCitation>
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)
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</emphasis>
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</heading>
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</paragraph>
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<paragraph id="8B8F36F8FFA52F14FF0153F4FD8EF8E2" blockId="13.[151,1436,1707,1858]" pageId="13" pageNumber="24">
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Valves linear-lanceolate,
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<quantity id="4CC89B1DFFA52F14FE3D53F4FDDEF962" box="[427,554,1707,1730]" metricMagnitude="-3" metricUnit="m" metricValue="3.6" metricValueMax="4.4" metricValueMin="2.8" pageId="13" pageNumber="24" unit="mm" value="3.6" valueMax="4.4" valueMin="2.8">2.8–4.4 µm</quantity>
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wide,
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<quantity id="4CC89B1DFFA52F14FDEC53F4FCE6F962" box="[634,786,1707,1730]" metricMagnitude="-2" metricUnit="m" metricValue="2.875" metricValueMax="3.3299999999999996" metricValueMin="2.42" pageId="13" pageNumber="24" unit="mm" value="28.75" valueMax="33.3" valueMin="24.2">24.2–33.3 µm</quantity>
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long, with rostrate to subcapitate ends. Striae transverse, uniseriate,
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<quantity id="4CC89B1DFFA52F14FEAB5394FE6FF942" box="[317,411,1739,1762]" metricMagnitude="0" metricUnit="m" metricValue="1.0922" metricValueMax="1.143" metricValueMin="1.0413999999999999" pageId="13" pageNumber="24" unit="in" value="43.0" valueMax="45.0" valueMin="41.0">41–45 in</quantity>
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<quantity id="4CC89B1DFFA52F14FE355394FE1FF942" box="[419,491,1739,1762]" metricMagnitude="-2" metricUnit="m" metricValue="1.0" pageId="13" pageNumber="24" unit="mm" value="10.0">10 µm</quantity>
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, comprising round areolae. Fibulae spaced approximately evenly along the keel,
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<quantity id="4CC89B1DFFA52F14FACC5394FF28F8A2" metricMagnitude="-1" metricUnit="m" metricValue="3.683" metricValueMax="4.318" metricValueMin="3.048" pageId="13" pageNumber="24" unit="in" value="14.5" valueMax="17.0" valueMin="12.0">12–17 in</quantity>
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<quantity id="4CC89B1DFFA52F14FF7053B4FEC6F8A2" box="[230,306,1771,1794]" metricMagnitude="-2" metricUnit="m" metricValue="1.0" pageId="13" pageNumber="24" unit="mm" value="10.0">10 µm</quantity>
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; raphe continuous, polar ends hook-shaped over valve apices; central node and central raphe endings absent (
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<figureCitation id="130B2A7DFFA52F14FE8C5254FE9FF882" box="[282,363,1803,1826]" captionStart="FIGURE 52–64" captionStartId="14.[151,244,1803,1824]" captionTargetBox="[151,1436,887,1782]" captionTargetId="figure-397@14.[151,1436,887,1782]" captionTargetPageId="14" captionText="FIGURE 52–64. Nitzschia palea, strain LCR-S:6:4:1, CHR618410. Scales: Fig. 52, 10 µm; Fig. 61, 5 µm (use for Figs 53–62); Fig. 63, 0.5 µm; Fig. 64, 1 µm. Fig. 52. Live cells viewed by LM. Figs 53–62. Cleaned frustules viewed by LM, showing size variation and raphe canals on opposite margins (Figs 53, 54 are single valves; raphe is visible in Fig. 53). Fig. 63. TEM of cleaned frustule, centre of valve, showing continuous raphe with no central termini." figureDoi="http://doi.org/10.5281/zenodo.5062016" httpUri="https://zenodo.org/record/5062016/files/figure.png" pageId="13" pageNumber="24">Fig. 63</figureCitation>
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). Living cells contained 2–4 plate-like parietal chloroplasts, arranged around the frustule perimeter and separated at the cell centre (
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<figureCitation id="130B2A7DFFA52F14FD8B5274FD99F8E2" box="[541,621,1835,1858]" captionStart="FIGURE 52–64" captionStartId="14.[151,244,1803,1824]" captionTargetBox="[151,1436,887,1782]" captionTargetId="figure-397@14.[151,1436,887,1782]" captionTargetPageId="14" captionText="FIGURE 52–64. Nitzschia palea, strain LCR-S:6:4:1, CHR618410. Scales: Fig. 52, 10 µm; Fig. 61, 5 µm (use for Figs 53–62); Fig. 63, 0.5 µm; Fig. 64, 1 µm. Fig. 52. Live cells viewed by LM. Figs 53–62. Cleaned frustules viewed by LM, showing size variation and raphe canals on opposite margins (Figs 53, 54 are single valves; raphe is visible in Fig. 53). Fig. 63. TEM of cleaned frustule, centre of valve, showing continuous raphe with no central termini." figureDoi="http://doi.org/10.5281/zenodo.5062016" httpUri="https://zenodo.org/record/5062016/files/figure.png" pageId="13" pageNumber="24">Fig. 52</figureCitation>
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).
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</paragraph>
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||
</subSubSection>
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||
<subSubSection id="C32A6573FFA52F14FF01522EFD57F879" pageId="13" pageNumber="24" type="description">
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<paragraph id="8B8F36F8FFA52F14FF01522EFCA0F82C" blockId="13.[151,1436,1905,2009]" box="[151,852,1905,1932]" pageId="13" pageNumber="24">
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<emphasis id="B944EAEAFFA52F14FF01522EFEE9F82B" bold="true" box="[151,285,1905,1931]" pageId="13" pageNumber="24">Reference:</emphasis>
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—
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<bibRefCitation id="EFA14B09FFA52F14FEAC522DFDE9F82C" author="Trobajo, R. & Clavero, E. & Chepurnov, V. A. & Sabbe, K. & Mann, D. G. & Ishihara, S. & Cox, E. J." box="[314,541,1906,1932]" pageId="13" pageNumber="24" pagination="443 - 459" refId="ref20591" refString="Trobajo, R., Clavero, E., Chepurnov, V. A., Sabbe, K., Mann, D. G., Ishihara, S. & Cox, E. J. (2009) Morphological, genetic and mating diversity within the widespread bioindicator Nitzschia palea (Bacillariophyceae). Phycologia 48: 443 - 459." type="journal article" year="2009">
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Trobajo
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<emphasis id="B944EAEAFFA52F14FE0B522DFE2CF82B" box="[413,472,1906,1931]" italics="true" pageId="13" pageNumber="24">et al.</emphasis>
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2009
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</bibRefCitation>
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, p. 449, table 3, figs 2–26.
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</paragraph>
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<paragraph id="8B8F36F8FFA52F14FF5052C7FD57F879" blockId="13.[151,1436,1905,2009]" pageId="13" pageNumber="24">
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<emphasis id="B944EAEAFFA52F14FF5052C7FE28F812" bold="true" box="[198,476,1944,1970]" pageId="13" pageNumber="24">Specimens examined:</emphasis>
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—CHR618410!, CHR618411!, CHR618412! (cleaned frustules from cultures LCR-S:6:4:1, 12:1:2, and 29:2 respectively).
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</paragraph>
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</subSubSection>
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<subSubSection id="C32A6573FFA62F16FF5055C8FDE4FCE3" lastPageId="15" lastPageNumber="26" pageId="14" pageNumber="25" type="distribution">
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<paragraph id="8B8F36F8FFA62F17FF5055C8FCE6FF5F" blockId="14.[151,1436,151,835]" pageId="14" pageNumber="25">
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<emphasis id="B944EAEAFFA62F17FF5055C8FE9DFF11" bold="true" box="[198,361,151,177]" pageId="14" pageNumber="25">Distribution:</emphasis>
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—The species is regarded as cosmopolitan and a useful bioindicator. However, the cryptic diversity discovered within it suggests that its growth preferences could currently be misunderstood. It appeared in cultures from 2 sites in the Styx (2 and 7).
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</paragraph>
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<paragraph id="8B8F36F8FFA62F17FF505454FDE4FCE3" blockId="14.[151,1436,151,835]" pageId="14" pageNumber="25">
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<emphasis id="B944EAEAFFA62F17FF505454FE65FE85" bold="true" box="[198,401,267,293]" pageId="14" pageNumber="25">Molecular data:</emphasis>
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—
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<emphasis id="B944EAEAFFA62F17FE395453FE28FE85" box="[431,476,268,293]" italics="true" pageId="14" pageNumber="25">Rbc</emphasis>
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L fragments were obtained for 3 strains from the Styx: strain LCR-S:6:4:1, 576 bp; strain LCR-S:12:1:2, 575 bp; and strain LCR-S:29:2, 571 bp. The first and last strains shared identical sequences; the other strain differed by 1 bp. The closest matches outside these strains were strain
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<collectingCountry id="F3277668FFA62F17FA9B5406FAA4FED3" box="[1293,1360,345,371]" name="Spain" pageId="14" pageNumber="25">Spain</collectingCountry>
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C and the diatom-derived endosymbiont of
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<emphasis id="B944EAEAFFA62F17FDC654DFFCD4FE39" box="[592,800,384,409]" italics="true" pageId="14" pageNumber="25">
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<taxonomicName id="4C304D7BFFA62F17FDC654DFFD32FE39" authority="Novis & Braidwood & Kilroy, 2012" authorityName="Novis & Braidwood & Kilroy" authorityYear="2012" box="[592,710,384,409]" class="Dinophyceae" family="Kryptoperidiniaceae" genus="Durinskia" higherTaxonomySource="GBIF" kingdom="Chromista" order="Peridiniales" pageId="14" pageNumber="42" phylum="Myzozoa" rank="genus" status="gen. nov.">Durinskia</taxonomicName>
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baltica
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</emphasis>
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(Levander)
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<bibRefCitation id="EFA14B09FFA62F17FC2554DFFB64FE3A" author="Carty, S. & Cox, E. R." box="[947,1168,384,410]" pageId="14" pageNumber="25" pagination="197 - 204" refId="ref17514" refString="Carty, S. & Cox, E. R. (1986) Kansodinium gen. nov. and Durinskia gen. nov.: two genera of freshwater dinoflagellates (Pyrrhophyta). Phycologia 25: 197 - 204." type="journal article" year="1986">Carty & Cox 1986</bibRefCitation>
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(p-distances = 0.009). However, the Styx strains formed a robust monophyletic group in both the Bayesian and MPB analyses, separate from all other strains of
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<taxonomicName id="4C304D7BFFA62F17FD8D5492FD8AFE46" authorityName="Smith" authorityYear="1856" baseAuthorityName="Kutz." box="[539,638,461,486]" class="Monogenea" family="Capsalidae" genus="Nitzschia" kingdom="Animalia" order="Capsalidea" pageId="14" pageNumber="25" phylum="Platyhelminthes" rank="species" species="palea">
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<emphasis id="B944EAEAFFA62F17FD8D5492FD8AFE46" box="[539,638,461,486]" italics="true" pageId="14" pageNumber="25">N. palea</emphasis>
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</taxonomicName>
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in Genbank (
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<figureCitation id="130B2A7DFFA62F17FC895492FC72FE47" box="[799,902,461,487]" captionStart="FIGURE 162" captionStartId="28.[151,244,1590,1611]" captionTargetBox="[154,1433,626,1529]" captionTargetId="figure-383@28.[151,1436,617,1569]" captionTargetPageId="28" captionText="FIGURE 162. Bayesian phylogenies of Nitzschia strains from the Styx River, for rbcL (left) and 18S rDNA (right). Support numbers above branches are Bayesian posterior probabilities / Maximum Parsimony bootstrap values. Scale bar = 0.1 subsitutions/site. See text for substitution models and description of data sets." figureDoi="http://doi.org/10.5281/zenodo.5062040" httpUri="https://zenodo.org/record/5062040/files/figure.png" pageId="14" pageNumber="25">Fig. 162</figureCitation>
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). The overall dataset was 1470 bp long, with 214 variable sites (103 parsimony informative, 42 occurring within the Styx fragments). The model chosen and implemented in the Bayesian analysis was T92+G. One fragment of 18S sequence was obtained, for strain LCR-S:6:4:1, 1243 bp. The closest matches to this sequence were the
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<taxonomicName id="4C304D7BFFA62F17FBB2571EFB73FDFA" authorityName="Smith" authorityYear="1856" baseAuthorityName="Kutz." box="[1060,1159,577,602]" class="Monogenea" family="Capsalidae" genus="Nitzschia" kingdom="Animalia" order="Capsalidea" pageId="14" pageNumber="25" phylum="Platyhelminthes" rank="species" species="palea">
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<emphasis id="B944EAEAFFA62F17FBB2571EFB73FDFA" box="[1060,1159,577,602]" italics="true" pageId="14" pageNumber="25">N. palea</emphasis>
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</taxonomicName>
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clones NPAL2, 3, 8, 9, and 12, and NZSP (from
|
||
<collectingCountry id="F3277668FFA62F17FE565737FDA3FD22" box="[448,599,616,642]" name="Luxembourg" pageId="14" pageNumber="25">Luxembourg</collectingCountry>
|
||
and
|
||
<collectingCountry id="F3277668FFA62F17FD065737FD06FD22" box="[656,754,616,642]" name="Ukraine" pageId="14" pageNumber="25">Ukraine</collectingCountry>
|
||
; all p-distance = 0.001). The p-distance between the Styx strain and the remaining
|
||
<specimenCount id="9D36FD71FFA62F17FE2857D1FE05FD08" box="[446,497,654,680]" pageId="14" pageNumber="25" type="generic">
|
||
3
|
||
<taxonomicName id="4C304D7BFFA62F17FE4357D0FE05FD08" authorityName="Smith" authorityYear="1856" baseAuthorityName="Kutz." box="[469,497,655,680]" class="Monogenea" family="Capsalidae" genus="Nitzschia" kingdom="Animalia" order="Capsalidea" pageId="14" pageNumber="25" phylum="Platyhelminthes" rank="species" species="palea">
|
||
<emphasis id="B944EAEAFFA62F17FE4357D0FE05FD08" box="[469,497,655,680]" italics="true" pageId="14" pageNumber="25">N.</emphasis>
|
||
</taxonomicName>
|
||
</specimenCount>
|
||
palea clones in the database was 0.002. The Styx strain formed a robust clade with all these clones in both Bayesian and MPB analyses, but relationships within this clade were not resolved (presumably due to insufficient variation). The overall dataset was 1707 bp long, with 91 variable sites (39 parsimony informative, 28 occurring in the Styx fragment). The model chosen and implemented in the Bayesian analysis was K2+G+I.
|
||
</paragraph>
|
||
<caption id="DF4F6670FFA62F17FF015254FBA9F813" ID-DOI="http://doi.org/10.5281/zenodo.5062016" ID-Zenodo-Dep="5062016" httpUri="https://zenodo.org/record/5062016/files/figure.png" pageId="14" pageNumber="25" startId="14.[151,244,1803,1824]" targetBox="[151,1436,887,1782]" targetPageId="14">
|
||
<paragraph id="8B8F36F8FFA62F17FF015254FE61F89E" blockId="14.[151,1436,1803,2004]" pageId="14" pageNumber="25">
|
||
<emphasis id="B944EAEAFFA62F17FF015254FEC8F880" bold="true" box="[151,316,1803,1824]" pageId="14" pageNumber="25">FIGURE 52–64.</emphasis>
|
||
<taxonomicName id="4C304D7BFFA62F17FED55253FE23F881" authorityName="Smith" authorityYear="1856" baseAuthorityName="Kutz." box="[323,471,1804,1825]" class="Monogenea" family="Capsalidae" genus="Nitzschia" kingdom="Animalia" order="Capsalidea" pageId="14" pageNumber="25" phylum="Platyhelminthes" rank="species" species="palea">
|
||
<emphasis id="B944EAEAFFA62F17FED55253FE23F881" box="[323,471,1804,1825]" italics="true" pageId="14" pageNumber="25">Nitzschia palea</emphasis>
|
||
</taxonomicName>
|
||
, strain LCR-S:6:4:1, CHR618410. Scales: Fig. 52, 10 µm; Fig. 61, 5 µm (use for Figs 53–62); Fig. 63, 0.5 µm; Fig. 64, 1 µm.
|
||
</paragraph>
|
||
<paragraph id="8B8F36F8FFA62F17FF015219FE14F8FB" blockId="14.[151,1436,1803,2004]" box="[151,480,1862,1883]" pageId="14" pageNumber="25">
|
||
<emphasis id="B944EAEAFFA62F17FF015219FF17F8FB" bold="true" box="[151,227,1862,1883]" pageId="14" pageNumber="25">Fig. 52.</emphasis>
|
||
Live cells viewed by LM.
|
||
</paragraph>
|
||
<paragraph id="8B8F36F8FFA62F17FF01523CFE17F836" blockId="14.[151,1436,1803,2004]" pageId="14" pageNumber="25">
|
||
<emphasis id="B944EAEAFFA62F17FF01523CFEF8F8D8" bold="true" box="[151,268,1891,1912]" pageId="14" pageNumber="25">Figs 53–62.</emphasis>
|
||
Cleaned frustules viewed by LM, showing size variation and raphe canals on opposite margins (Figs 53, 54 are single valves; raphe is visible in Fig. 53).
|
||
</paragraph>
|
||
<paragraph id="8B8F36F8FFA62F17FF0152C1FBA9F813" blockId="14.[151,1436,1803,2004]" box="[151,1117,1950,1971]" pageId="14" pageNumber="25">
|
||
<emphasis id="B944EAEAFFA62F17FF0152C1FF17F813" bold="true" box="[151,227,1950,1971]" pageId="14" pageNumber="25">Fig. 63.</emphasis>
|
||
TEM of cleaned frustule, centre of valve, showing continuous raphe with no central termini.
|
||
</paragraph>
|
||
</caption>
|
||
<paragraph id="8B8F36F8FFA62F17FF0152E0FC46F874" blockId="14.[151,1436,1803,2004]" box="[151,946,1983,2004]" pageId="14" pageNumber="25">
|
||
<emphasis id="B944EAEAFFA62F17FF0152E0FF17F874" bold="true" box="[151,227,1983,2004]" pageId="14" pageNumber="25">
|
||
<figureCitation id="130B2A7DFFA62F17FF0152E0FF29F874" box="[151,221,1983,2004]" captionStart="FIGURE 52–64" captionStartId="14.[151,244,1803,1824]" captionTargetBox="[151,1436,887,1782]" captionTargetId="figure-397@14.[151,1436,887,1782]" captionTargetPageId="14" captionText="FIGURE 52–64. Nitzschia palea, strain LCR-S:6:4:1, CHR618410. Scales: Fig. 52, 10 µm; Fig. 61, 5 µm (use for Figs 53–62); Fig. 63, 0.5 µm; Fig. 64, 1 µm. Fig. 52. Live cells viewed by LM. Figs 53–62. Cleaned frustules viewed by LM, showing size variation and raphe canals on opposite margins (Figs 53, 54 are single valves; raphe is visible in Fig. 53). Fig. 63. TEM of cleaned frustule, centre of valve, showing continuous raphe with no central termini." figureDoi="http://doi.org/10.5281/zenodo.5062016" httpUri="https://zenodo.org/record/5062016/files/figure.png" pageId="14" pageNumber="25">Fig. 64</figureCitation>
|
||
.
|
||
</emphasis>
|
||
SEM of cleaned frustule, showing fine areolae and lack of median fibulae.
|
||
</paragraph>
|
||
<paragraph id="8B8F36F8FFA72F16FF5055C8FCBCFD22" blockId="15.[151,1436,151,642]" pageId="15" pageNumber="26">
|
||
<emphasis id="B944EAEAFFA72F16FF5055C8FE98FF11" bold="true" box="[198,364,151,177]" pageId="15" pageNumber="26">Observations</emphasis>
|
||
:—The morphology of the Styx strains is closest to the strain
|
||
<collectingCountry id="F3277668FFA72F16FBCD55C7FB69FF12" box="[1115,1181,152,178]" name="Japan" pageId="15" pageNumber="26">Japan</collectingCountry>
|
||
A2 of
|
||
<bibRefCitation id="EFA14B09FFA72F16FB6F55C7FF10FF78" author="Trobajo, R. & Clavero, E. & Chepurnov, V. A. & Sabbe, K. & Mann, D. G. & Ishihara, S. & Cox, E. J." pageId="15" pageNumber="26" pagination="443 - 459" refId="ref20591" refString="Trobajo, R., Clavero, E., Chepurnov, V. A., Sabbe, K., Mann, D. G., Ishihara, S. & Cox, E. J. (2009) Morphological, genetic and mating diversity within the widespread bioindicator Nitzschia palea (Bacillariophyceae). Phycologia 48: 443 - 459." type="journal article" year="2009">
|
||
Trobajo
|
||
<emphasis id="B944EAEAFFA72F16FAC855C7FA68FF11" box="[1374,1436,152,177]" italics="true" pageId="15" pageNumber="26">et al.</emphasis>
|
||
(2009)
|
||
</bibRefCitation>
|
||
in terms of fibulae and striae per
|
||
<quantity id="4CC89B1DFFA72F16FDF955E1FD49FF78" box="[623,701,190,216]" metricMagnitude="-2" metricUnit="m" metricValue="1.0" pageId="15" pageNumber="26" unit="mm" value="10.0">10 µm</quantity>
|
||
, and closest to strain
|
||
<collectingCountry id="F3277668FFA72F16FC2E55E1FC0EFF78" box="[952,1018,190,216]" name="Spain" pageId="15" pageNumber="26">Spain</collectingCountry>
|
||
A
|
||
<quantity id="4CC89B1DFFA72F16FB8E55E1FBB0FF78" box="[1048,1092,190,216]" metricMagnitude="-2" metricUnit="m" metricValue="7.62" pageId="15" pageNumber="26" unit="in" value="3.0">3 in</quantity>
|
||
length range. Unfortunately, an
|
||
<emphasis id="B944EAEAFFA72F16FF2A55BAFF10FF5E" box="[188,228,229,254]" italics="true" pageId="15" pageNumber="26">rbc</emphasis>
|
||
L sequence for
|
||
<collectingCountry id="F3277668FFA72F16FE3E55BAFE1AFF5F" box="[424,494,229,255]" name="Japan" pageId="15" pageNumber="26">Japan</collectingCountry>
|
||
A2 is not available, and
|
||
<collectingCountry id="F3277668FFA72F16FCBC55BAFC9BFF5F" box="[810,879,229,255]" name="Spain" pageId="15" pageNumber="26">Spain</collectingCountry>
|
||
A3 is not united with the Styx strains in the phylogenetic analysis. One possible conclusion from our molecular analyses is that the Styx strains form a monophyletic group as an artefact of their short sequence lengths; however, analyses in which the dataset is completely cropped to the length of the Styx sequences maintain the relationships.
|
||
<bibRefCitation id="EFA14B09FFA72F16FBF45406FAB8FED3" author="Trobajo, R. & Clavero, E. & Chepurnov, V. A. & Sabbe, K. & Mann, D. G. & Ishihara, S. & Cox, E. J." box="[1122,1356,345,371]" pageId="15" pageNumber="26" pagination="443 - 459" refId="ref20591" refString="Trobajo, R., Clavero, E., Chepurnov, V. A., Sabbe, K., Mann, D. G., Ishihara, S. & Cox, E. J. (2009) Morphological, genetic and mating diversity within the widespread bioindicator Nitzschia palea (Bacillariophyceae). Phycologia 48: 443 - 459." type="journal article" year="2009">
|
||
Trobajo
|
||
<emphasis id="B944EAEAFFA72F16FB525406FB0AFED2" box="[1220,1278,345,370]" italics="true" pageId="15" pageNumber="26">et al.</emphasis>
|
||
(2009
|
||
</bibRefCitation>
|
||
,
|
||
<bibRefCitation id="EFA14B09FFA72F16FACE5406FA60FED3" author="Trobajo, R. & Mann, D. G. & Clavero, E. & Evans, K. M. & Vanormelingan, P. & McGregor, R. C." box="[1368,1428,345,371]" pageId="15" pageNumber="26" pagination="413 - 425" refId="ref20657" refString="Trobajo, R., Mann, D. G., Clavero, E., Evans, K. M., Vanormelingan, P., McGregor, R. C. (2010) The use of partial cox 1, rbc L and LSU rDNA sequences for phylogenetics and species identification within the Nitzschia palea species complex (Bacillariophyceae). European Journal of Phycology 45: 413 - 425." type="journal article" year="2010">2010</bibRefCitation>
|
||
) propose that
|
||
<taxonomicName id="4C304D7BFFA72F16FEA154DFFE6BFE39" authorityName="Smith" authorityYear="1856" baseAuthorityName="Kutz." box="[311,415,384,409]" class="Monogenea" family="Capsalidae" genus="Nitzschia" kingdom="Animalia" order="Capsalidea" pageId="15" pageNumber="26" phylum="Platyhelminthes" rank="species" species="palea">
|
||
<emphasis id="B944EAEAFFA72F16FEA154DFFE6BFE39" box="[311,415,384,409]" italics="true" pageId="15" pageNumber="26">N. palea</emphasis>
|
||
</taxonomicName>
|
||
will need to be split into 3 or more species; problematically, this seems to require species to be defined purely according to molecular data. This would create serious problems for those required to identify these diatoms by LM or even SEM (i.e. most identifications), leading to the continued use of the name
|
||
<taxonomicName id="4C304D7BFFA72F16FEBA54ABFE64FDAD" authorityName="Smith" authorityYear="1856" baseAuthorityName="Kutz." box="[300,400,500,525]" class="Monogenea" family="Capsalidae" genus="Nitzschia" kingdom="Animalia" order="Capsalidea" pageId="15" pageNumber="26" phylum="Platyhelminthes" rank="species" species="palea">
|
||
<emphasis id="B944EAEAFFA72F16FEBA54ABFE64FDAD" box="[300,400,500,525]" italics="true" pageId="15" pageNumber="26">N. palea</emphasis>
|
||
</taxonomicName>
|
||
by default. This species shows the most convincing biogeographic structure of all the taxa determined in this study, thanks to its relatively extensive sampling. However, it is possible that the
|
||
<collectingCountry id="F3277668FFA72F16FAE45745FA68FD94" box="[1394,1436,538,564]" name="United Kingdom" pageId="15" pageNumber="26">UK</collectingCountry>
|
||
strain of
|
||
<bibRefCitation id="EFA14B09FFA72F16FE96571EFE00FDFB" author="Trobajo, R. & Mann, D. G. & Clavero, E. & Evans, K. M. & Vanormelingan, P. & McGregor, R. C." box="[256,500,577,603]" pageId="15" pageNumber="26" pagination="413 - 425" refId="ref20657" refString="Trobajo, R., Mann, D. G., Clavero, E., Evans, K. M., Vanormelingan, P., McGregor, R. C. (2010) The use of partial cox 1, rbc L and LSU rDNA sequences for phylogenetics and species identification within the Nitzschia palea species complex (Bacillariophyceae). European Journal of Phycology 45: 413 - 425." type="journal article" year="2010">
|
||
Trobajo
|
||
<emphasis id="B944EAEAFFA72F16FEF5571EFE6AFDFA" box="[355,414,577,602]" italics="true" pageId="15" pageNumber="26">et al.</emphasis>
|
||
(2010)
|
||
</bibRefCitation>
|
||
would form a clade with the Styx strains, since it occupies a similar position in a
|
||
<emphasis id="B944EAEAFFA72F16FF3D5737FF27FD21" box="[171,211,616,641]" italics="true" pageId="15" pageNumber="26">cox</emphasis>
|
||
1 analysis (
|
||
<emphasis id="B944EAEAFFA72F16FECF5737FE74FD21" box="[345,384,616,641]" italics="true" pageId="15" pageNumber="26">rbc</emphasis>
|
||
L data are not available for this strain).
|
||
</paragraph>
|
||
<paragraph id="8B8F36F8FFA72F16FF015784FE64FD58" blockId="15.[151,400,731,760]" box="[151,400,731,760]" pageId="15" pageNumber="26">
|
||
Order
|
||
<taxonomicName id="4C304D7BFFA72F16FF7F5784FE64FD58" box="[233,400,731,760]" class="Bacillariophyceae" higherTaxonomySource="GBIF" kingdom="Chromista" order="Cymbellales" pageId="15" pageNumber="26" phylum="Bacillariophyta" rank="order">
|
||
<emphasis id="B944EAEAFFA72F16FF7F5784FE64FD58" bold="true" box="[233,400,731,760]" pageId="15" pageNumber="26">Cymbellales</emphasis>
|
||
</taxonomicName>
|
||
</paragraph>
|
||
<paragraph id="8B8F36F8FFA72F16FF015679FDE4FCE3" blockId="15.[151,528,806,835]" box="[151,528,806,835]" pageId="15" pageNumber="26">
|
||
Family
|
||
<taxonomicName id="4C304D7BFFA72F16FF6F5679FDE4FCE3" authorityName="F.T.Kutzing" authorityYear="1844" box="[249,528,806,835]" class="Bacillariophyceae" family="Gomphonemataceae" higherTaxonomySource="GBIF" kingdom="Chromista" order="Cymbellales" pageId="15" pageNumber="26" phylum="Bacillariophyta" rank="family">
|
||
<emphasis id="B944EAEAFFA72F16FF6F5679FDE4FCE3" bold="true" box="[249,528,806,835]" pageId="15" pageNumber="26">Gomphonemataceae</emphasis>
|
||
</taxonomicName>
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |