352 lines
36 KiB
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352 lines
36 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.99.90117" ID-Pensoft-Pub="1314-4049-99-45" ID-Pensoft-UUID="47D9DA3E1C56567CA14356476272F738" ModsDocID="1314-4049-99-45" checkinTime="1692098463846" checkinUser="pensoft" docAuthor="Itagaki, Hiyori & Hosoya, Tsuyoshi" docDate="2023" docId="685E05E945D055BF8AA56A7EEF95BD73" docLanguage="en" docName="MycoKeys 99: 45-85" docOrigin="MycoKeys 99" docPubDate="2023-08-14" docSource="http://dx.doi.org/10.3897/mycokeys.99.90117" docTitle="Neobelonopsis multiguttata Itagaki & Hosoya 2023, sp. nov." docType="treatment" docVersion="1" id="47D9DA3E1C56567CA14356476272F738" lastPageNumber="45" masterDocId="47D9DA3E1C56567CA14356476272F738" masterDocTitle="A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan" masterLastPageNumber="85" masterPageNumber="45" pageNumber="45" updateTime="1692098463846" updateUser="pensoft">
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<mods:titleInfo id="7680665CB487CA4EC7AC0D368089AE7D">
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<mods:title id="10176A56F48A908DBC4B8F9B7DFED4EC">A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan</mods:title>
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<mods:name id="CB8BC5461FF1E7D930EF1F2A4056AEE3" type="personal">
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<mods:roleTerm id="A1EEE7D7FAF8EEC1D34341AB151B87C3">Author</mods:roleTerm>
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<mods:namePart id="B6D2B32390F2E7843E271C7153A74072">Itagaki, Hiyori</mods:namePart>
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<mods:nameIdentifier id="F5CEFC84BBACBE7E2072B50628C77442" type="ORCID">https://orcid.org/0000-0001-8678-0826</mods:nameIdentifier>
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<mods:affiliation id="1D078A5F3D1F07BD1E25E53EF782C695">Department of Botany, National Museum of Nature and Science, 4 - 1 - 1, Amakubo, Tsukuba, Ibaraki, 305 - 0005, Japan</mods:affiliation>
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<mods:nameIdentifier id="6B51498D0BAE621D73BC7830D4ED6186" type="email">itagaki@kahaku.go.jp</mods:nameIdentifier>
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<mods:name id="0342A73DD1C3BF50B029DC41F1437D9F" type="personal">
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</mods:role>
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<mods:namePart id="B5C906C5217D51948AD066129BFED93D">Hosoya, Tsuyoshi</mods:namePart>
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<mods:nameIdentifier id="7B8BA380519D9588A31F43EF20A38B90" type="ORCID">https://orcid.org/0000-0001-5360-5677</mods:nameIdentifier>
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<mods:affiliation id="B5861E3ECA5EEB51C8A2958D2D1C69D3">Department of Botany, National Museum of Nature and Science, 4 - 1 - 1, Amakubo, Tsukuba, Ibaraki, 305 - 0005, Japan</mods:affiliation>
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<mods:typeOfResource id="65E58544DFF5220961D4443CE383073D">text</mods:typeOfResource>
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<mods:title id="1327466CCC84387D324D7E98DAC0D932">MycoKeys</mods:title>
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<mods:date id="8A457B1E5F0D35FE9F53CBD4A1724E20">2023</mods:date>
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<mods:number id="89DDB67B45840D933E974D30557747E3">2023-08-14</mods:number>
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<mods:number id="831C7361590D5DB3DAFB84322BF5B9D2">99</mods:number>
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<mods:start id="FD0D8E787D1B2E6E8A2F5B8BF7114FDD">45</mods:start>
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<mods:url id="6FD18BE3449A566A2D0B3586837EE66B">http://dx.doi.org/10.3897/mycokeys.99.90117</mods:url>
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<mods:classification id="CF30E59C35E9E3F671D37B1501641A14">journal article</mods:classification>
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<mods:identifier id="0D7A5CF4DD35AEE0DF3C7505558F56EC" type="DOI">http://dx.doi.org/10.3897/mycokeys.99.90117</mods:identifier>
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<mods:identifier id="FDC02A48240DDD806290CBE12800C0C3" type="Pensoft-Pub">1314-4049-99-45</mods:identifier>
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<treatment id="685E05E945D055BF8AA56A7EEF95BD73" LSID="urn:lsid:plazi:treatment:685E05E945D055BF8AA56A7EEF95BD73" httpUri="http://treatment.plazi.org/id/685E05E945D055BF8AA56A7EEF95BD73" lastPageNumber="45" pageId="0" pageNumber="45">
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<subSubSection id="982A5AD062EA7E908345CBEEB8831EAB" pageId="0" pageNumber="45" type="nomenclature">
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<paragraph id="FE0A98EE4D03631D12BB1F86931DDFCB" pageId="0" pageNumber="45">
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<taxonomicName id="EBEC1BD5029574B3982B7271AE0016D2" LSID="685E05E9-45D0-55BF-8AA5-6A7EEF95BD73" authority="Itagaki & Hosoya" authorityName="Itagaki & Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" higherTaxonomySource="treatment-meta" kingdom="Fungi" lsidName="Neobelonopsis multiguttata" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="multiguttata" status="sp. nov.">Neobelonopsis multiguttata Itagaki & Hosoya</taxonomicName>
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<taxonomicNameLabel id="4E5BA6A56710A846C9BCE48F6D750A9D" pageId="0" pageNumber="45">sp. nov.</taxonomicNameLabel>
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</paragraph>
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||
</subSubSection>
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||
<subSubSection id="BBC13E77E5ABC8C57836321A26903F62" pageId="0" pageNumber="45" type="description">
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<paragraph id="5D6398F215A3F327D74F925384A7679F" pageId="0" pageNumber="45">
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<figureCitation id="19FBE1693D4690D17F4DD5A724ACC893" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Neobelonopsis multiguttata (TNS-F- 86402, holotype) A fresh apothecia on decaying culm of Sasa kurilensis B dried apothecia C stromata with sparse subiculum D vertical section of apothecium (in LA) E ascospores (in CB / LA) F asci with ascospores (in CB / LA) G paraphyses (in CB / LA) H blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) I croziers at the base of asci (in CB / LA) J texture of stroma (in LA) K subicular hyphae (in lactic acid) L three months old colony on PDA M conidia (in CB / LA) N-R conidiophores, arrows in N indicated conidiogenous cells (in CB / LA). Scale bars: 0.5 mm (A-C); 50 μm (D, K); 20 μm (N), 10 μm (E-J, M, O-R)." figureDoi="10.3897/mycokeys.99.90117.figure8" httpUri="https://binary.pensoft.net/fig/892424" pageId="0" pageNumber="45">Figs 8</figureCitation>
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<figureCitation id="326DF6C50469228BF55EF3799ED429D6" captionStart="Figure 13" captionStartId="F13" captionText="Figure 13. Line-drawings of the Neobelonopsis and Trichobelonium species I vertical section of the apothecium showing the marginal structure of ectal excipulum, medullary excipulum, and hymenium II asci III paraphyses IV ascospores. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure13" httpUri="https://binary.pensoft.net/fig/892429" pageId="0" pageNumber="45">, 13</figureCitation>
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<figureCitation id="AF5DF8045FAEBC551A89E9E0AF8CB6AE" captionStart="Figure 14" captionStartId="F14" captionText="Figure 14. Asexual morph and hyphal structures of the Neobelonopsis and Trichobelonium species A conidiophores of N. acutata B monilioid hyphae of N. bicolor C conidiophores with conidia of N. cinnabarina D conidiophores with conidia of N. didymospora E conidiophores with conidia of N. microspora F conidiophores with conidia of N. multiguttata G conidiophores with conidia of N. obtusa H conidiophores with conidia of T. albobarbatum I hyphal mass with melanized appressorium-like structure of T. miscanthi. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure14" httpUri="https://binary.pensoft.net/fig/892430" pageId="0" pageNumber="45">, 14F</figureCitation>
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</paragraph>
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||
</subSubSection>
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||
<subSubSection id="FFFCE013DB82E6228C80A2E37C1BB349" pageId="0" pageNumber="45" type="etymology">
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<paragraph id="50DC3C476C5EF7AE99BC1D100FA659ED" pageId="0" pageNumber="45">Etymology.</paragraph>
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<paragraph id="BDD3D3B37DCCF54BD84680AFB6ABA445" pageId="0" pageNumber="45">Named after the abundant number of guttules in the ascospores.</paragraph>
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</subSubSection>
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<subSubSection id="B2A304C6E2DFFE45B887B5F2F58F8945" pageId="0" pageNumber="45" type="diagnosis">
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<paragraph id="07FB4C566D032D12A8CAB7DA0B54196F" pageId="0" pageNumber="45">Diagnosis.</paragraph>
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<paragraph id="CAA0E489E847D6CE8270CEEF4FB1848D" pageId="0" pageNumber="45">
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Resembles
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<taxonomicName id="C2375B1C8CBC1F9EDF6FEAA28CD7E03C" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. acutata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="acutata">
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<emphasis id="C2D00D483E768CB665FD7606A1FD9D4A" italics="true" pageId="0" pageNumber="45">N. acutata</emphasis>
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</taxonomicName>
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, but distinguishable by more sparsely formed conidiophores, longer asci, and longer ascospores with rounded extremes (vs. more acute in
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<taxonomicName id="95CC7D68FD767086EA4DF26F2DB33228" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. acutata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="acutata">
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<emphasis id="DCB27591E178A49532B1F3DBA1BEF6D3" italics="true" pageId="0" pageNumber="45">N. acutata</emphasis>
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</taxonomicName>
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).
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</paragraph>
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</subSubSection>
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<subSubSection id="218355F55ACAED88EACF414AC2418179" pageId="0" pageNumber="45" type="holotype">
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<paragraph id="8E500B6D1C3665FD962C52414A6A7E3C" pageId="0" pageNumber="45">Holotype.</paragraph>
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<paragraph id="8D92135BBE128BFB54811FA5C6D1EFF7" pageId="0" pageNumber="45">
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TNS-F-86402, Sugadaira Research Station, Mountain Science Center, Ueda City, Nagano Pref., 5 June 2021, on decaying culms of
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<taxonomicName id="3664C21F80F032FA1A7223BD1CFA481C" authorityName="Makino & Shibata" authorityYear="1901" baseAuthorityName="Rupr." class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa kurilensis" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="kurilensis">
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<emphasis id="52C4CEB3B3C855C36D1E78C6CD8A822A" italics="true" pageId="0" pageNumber="45">Sasa kurilensis</emphasis>
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</taxonomicName>
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, ex-holotype culture NBRC 115371.
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</paragraph>
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</subSubSection>
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<subSubSection id="B583D2FAF86C17699A4A0B9952ECD8A6" pageId="0" pageNumber="45" type="description">
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<paragraph id="F4D7A7E80F9C061D95D5C3712343B079" pageId="0" pageNumber="45">Description.</paragraph>
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<paragraph id="BC7FFE6423A6F3358645D7705B707EC5" pageId="0" pageNumber="45">
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<emphasis id="B4A84941CCF99E69824EDD79EB6FDC56" bold="true" italics="true" pageId="0" pageNumber="45">Apothecia</emphasis>
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developed from scuta.
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<emphasis id="21141C3FA3516DE302C39D01721BF0CB" bold="true" italics="true" pageId="0" pageNumber="45">Scuta</emphasis>
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superficial, scattered to gregarious, flat discoid, approximately 0.2 mm diam., blackish brown (C80M100Y80-100K60),
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<emphasis id="A5A2E55C4C181DA21042E3AF05903890" bold="true" italics="true" pageId="0" pageNumber="45">textura epidermoidea</emphasis>
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, consisting of thick-walled cells.
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<emphasis id="B15C65172E011BFB030FFFF4AEE85A5F" bold="true" italics="true" pageId="0" pageNumber="45">Apothecia</emphasis>
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0.2-0.4 mm high, with dark brown (C80M80Y80-100K60) receptacle; disc 0.5-1.6 mm diam., white to pale gray (K10) when fresh, shrunk to 0.4-1.3 mm diam., pale yellow (Y10) when dried. Ectal excipulum 37-50
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<normalizedToken id="8AE4ED07065EB3B5F8ABF1318AC7FC44" originalValue="µm">µm</normalizedToken>
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thick at base, 25-35
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<normalizedToken id="7995DBF40977FB57486C9BAF55C24F6A" originalValue="µm">µm</normalizedToken>
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thick at the upper flank to margin; cortical cells hemispherical to short clavate, 12-17
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<normalizedToken id="BE0C149D629256DE43501C7D81FEC2D6" originalValue="×">x</normalizedToken>
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9-10(-12)
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<normalizedToken id="A10F55F66C0F35C0C82BC5314D5E888A" originalValue="µm">µm</normalizedToken>
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at base, becoming slender and closely packed toward the upper flank to margin. Medullary excipulum 37-87
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<normalizedToken id="6C450CFA9B632C3DC2470CBB538CE670" originalValue="µm">µm</normalizedToken>
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thick.
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<emphasis id="3BFA3B989041707537C6E3BA3C726BE9" bold="true" italics="true" pageId="0" pageNumber="45">Asci</emphasis>
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(63-)78-98(-105)
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<normalizedToken id="0112CE7ED56C2B324ABC88BA560477AF" originalValue="×">x</normalizedToken>
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5-8
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<normalizedToken id="CF94CCF33C800360F683A933B5EEAB34" originalValue="µm">µm</normalizedToken>
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, arising from croziers, with MLZ + apical pore.
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<emphasis id="264A404C64B4A6C67FFF9A41ED3BF92F" bold="true" italics="true" pageId="0" pageNumber="45">Ascospores</emphasis>
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(12-)17-26(-27.5)
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<normalizedToken id="500C92E8BFDA400106B7E4597F741111" originalValue="×">x</normalizedToken>
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2.5-3.5
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<normalizedToken id="5943BE9446412A01ACB667095CA6E17E" originalValue="µm">µm</normalizedToken>
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, long ellipsoid to fusiform with rounded extremes, (1-)3-septate, containing abundant guttules.
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<emphasis id="CEE6327129EFBDED477832304EB5BD1C" bold="true" italics="true" pageId="0" pageNumber="45">Paraphyses</emphasis>
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(62-)74-90(-100)
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<normalizedToken id="AEC515BCE80FF23858176E27C41FE9A9" originalValue="×">x</normalizedToken>
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2.5-3
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<normalizedToken id="E9FC0CA64EFE967D10A0240B3DF9B39F" originalValue="µm">µm</normalizedToken>
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, simple, with long apical cell.
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<emphasis id="7425E793A627485796C390DD1D900880" bold="true" italics="true" pageId="0" pageNumber="45">Subiculum</emphasis>
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sparsely developed, covering the surface of substrates in patches, shiny brown; subicular hyphae straight to curved, usually constricted at septum, fascicular, 3-5
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<normalizedToken id="717FC60EFC51127232B43C97A7CD4FF2" originalValue="µm">µm</normalizedToken>
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width with 0.5-1
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<normalizedToken id="8A2DA52CE84AD25780ED91B9F6C3A0F1" originalValue="µm">µm</normalizedToken>
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thick-walls, septate every 15-25(-50)
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<normalizedToken id="ADD9C658A8032B4C61C3B16B14EF688B" originalValue="µm">µm</normalizedToken>
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, branched at right angle, covered by gelatinous substance.
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<emphasis id="3C535D0B7C082A205C78E3C83AA5145B" bold="true" italics="true" pageId="0" pageNumber="45">Colony</emphasis>
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of NBRC 115371 on PDA flat to slightly winkled, entire to undulate, floccose to woolly, grayish brown (C20-30M40Y40K60) from the surface, forming indistinct section observed clearer from the reverse, without soluble pigment and crystals; aerial mycelium moderately abundant at the center, sparse at the edge, pale gray (K10-30) to white.
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<emphasis id="FD30515FDA6DA266A5E6A5460512BC78" bold="true" italics="true" pageId="0" pageNumber="45">Conidiophores</emphasis>
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semi-macronematous, short, arising vertically from aerial hyphae, pale to dark brown, smooth, thick-walled, constricted, occasionally loosely branched;
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<emphasis id="1BB14AA3C7F98CABB0566966AA795F65" bold="true" italics="true" pageId="0" pageNumber="45">phialides</emphasis>
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cylindrical to ampulliform, up to 16
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<normalizedToken id="3BA915D40913D1D04C91A5721D80A33E" originalValue="µm">µm</normalizedToken>
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long, 3
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<normalizedToken id="467047C2DC9AE466DD4C3C6A698CEBA5" originalValue="µm">µm</normalizedToken>
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width at base, discrete, arranged terminal or intercalary, pale brown, thick-walled, with cylindrical funnel-shape collarettes of 4.5-6.5
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<normalizedToken id="BD94A75D7DA4259CC2ACC2E99569BB7C" originalValue="×">x</normalizedToken>
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2-3
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<normalizedToken id="6D548EE778EE6DFDBEB96AC2E4DF6047" originalValue="µm">µm</normalizedToken>
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;
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<emphasis id="1D012ECBCB950752FBBEE8073E5CE0B7" bold="true" italics="true" pageId="0" pageNumber="45">conidia</emphasis>
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aseptate, spherical to subspherical, abundantly aggregated in slimy heads, 1.5-1.7
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<normalizedToken id="5B8B5CF39E8B33993DEB95B832BE62D9" originalValue="µm">µm</normalizedToken>
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diam., hyaline, thin-walled.
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</paragraph>
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</subSubSection>
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<subSubSection id="C1CDD2F0F2D8EC2AC80AF922A99F0AA4" pageId="0" pageNumber="45" type="materials_examined">
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<paragraph id="EB2B97D0F4ED809DCB5E0B9286F0C85C" pageId="0" pageNumber="45">Additional specimens examined.</paragraph>
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<paragraph id="C4CF26887749C813A5DE7849C338CBF5" pageId="0" pageNumber="45">
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TNS-F-18023, Shirakamisanchi, Aomori Pref.,
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<date id="42843291F16026C43FEE50644E33B34F" value="2006-05-24">24 May 2006</date>
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, on decaying culms of
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<taxonomicName id="959EDF95838B3EB203E2445722F40F46" class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="genus">
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<emphasis id="A56515FF8580F2420D79773BF7DF0A1A" italics="true" pageId="0" pageNumber="45">Sasa</emphasis>
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</taxonomicName>
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sp.; TNS-F-39229, Mt. Tsukuba, Tsukuba City, Ibaraki Pref.,
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<date id="C6FE45D6F296A7A48F31B84ADFA0F951" value="2011-04-22">22 April 2011</date>
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, on decaying culms of
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<taxonomicName id="C57C28F9E8D8E11BF28662E616913435" class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="genus">
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<emphasis id="A8C4CCC5D96E073BE18DD0597F69289B" italics="true" pageId="0" pageNumber="45">Sasa</emphasis>
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</taxonomicName>
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sp.; TNS-F-54941, Omama Town, Midori City, Gunma Pref.,
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<date id="3496F4C03D4B7E4EC8AA3DE8D9C62B12" value="2018-05-09">9 May 2018</date>
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, on decaying culms of
|
||
<taxonomicName id="7E51D817AFCE90EAAD301F326C63125A" class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="genus">
|
||
<emphasis id="F307C252362E03576B3AF7574EC612A7" italics="true" pageId="0" pageNumber="45">Sasa</emphasis>
|
||
</taxonomicName>
|
||
sp.; TNS-F-61278, Mt. Tsukuba, Tsukuba City, Ibaraki Pref.,
|
||
<date id="DF78F4D981ECD25B5362B1342D9DCF0E" value="2014-04-16">16 April 2014</date>
|
||
, on fallen cupules of
|
||
<taxonomicName id="B9390EE9D5104286075021304F2A898E" class="Dicotyledoneae" family="Fagaceae" genus="Fagus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Fagus crenata" order="Fagales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="crenata">
|
||
<emphasis id="F0F9A9779EC5A874B8999A09016C30CA" italics="true" pageId="0" pageNumber="45">Fagus crenata</emphasis>
|
||
</taxonomicName>
|
||
; TNS-F-61280, Hakone Town, Ashigara-shimo County, Kanagawa Pref.,
|
||
<date id="FE44E74EDC496EF8A17337D388D2924F" value="2014-05-23">23 May 2014</date>
|
||
, on fallen cupules of
|
||
<taxonomicName id="A6C98D81DD3A1AC973E50A03C83BC677" lsidName="F. crenata" pageId="0" pageNumber="45" rank="species" species="crenata">
|
||
<emphasis id="0AA97E3D6D46E385984B0BD307DCA4C0" italics="true" pageId="0" pageNumber="45">F. crenata</emphasis>
|
||
</taxonomicName>
|
||
Blume, culture NBRC 115655; TNS-F-81133, Sugadaira Research Station, Montane Science Center, Ueda City, Nagano Pref.,
|
||
<date id="AC2D64449C2E9AB8CCCEB1F4E8929AF7" value="2017-06">June 2017</date>
|
||
, on decaying culms of
|
||
<taxonomicName id="9177B3169B600973A98C1B7127ECECA4" authorityName="Makino & Shibata" authorityYear="1901" baseAuthorityName="Rupr." class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa kurilensis" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="kurilensis">
|
||
<emphasis id="56FE0442B4D1BD4230AC8EC146964F71" italics="true" pageId="0" pageNumber="45">Sasa kurilensis</emphasis>
|
||
</taxonomicName>
|
||
; TNS-F-86224, Sekimoto Town, Kita-ibaraki City, Ibaraki Pref.,
|
||
<date id="C730AF730B710BAEF4214590B1E0C12B" value="2020-06-03">3 June 2020</date>
|
||
, on dead branches on living
|
||
<taxonomicName id="E26801C11E5C90DA4A4D64557729C1A9" authorityName="Siebold & Zucc. ex Zabel" authorityYear="1885" baseAuthorityName="Thunb." class="Dicotyledoneae" family="Rosaceae" genus="Stephanandra" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Stephanandra incisa" order="Rosales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="incisa">
|
||
<emphasis id="A31262E9D4488F48DCEB6E03E6971C5E" italics="true" pageId="0" pageNumber="45">Stephanandra incisa</emphasis>
|
||
</taxonomicName>
|
||
, culture NBRC 115365; TNS-F-86426, Mt. Amari, Asahi Town, Nirasaki City, Yamanashi Pref.,
|
||
<date id="6E56B2230C4B1588DDBA14430EF18232" value="2021-06-14">14 June 2021</date>
|
||
, on decaying culms of
|
||
<taxonomicName id="19330362AD91AC0F03DA540F945162C9" class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="genus">
|
||
<emphasis id="7019CCFB6E24CE89283E054462D61168" italics="true" pageId="0" pageNumber="45">Sasa</emphasis>
|
||
</taxonomicName>
|
||
sp.; TNS-F-86465, Nagataki Town, Noumi City, Ishikawa Pref.,
|
||
<date id="475AE6EF7E84A49656A1E8BA573B04F3" value="2021-06-18">18 June 2021</date>
|
||
, on decaying culms of
|
||
<taxonomicName id="7F2EB50EF59BAEC804CD91B5858E6FE9" class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa palmata" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="palmata">
|
||
<emphasis id="3B5CF8CFC60BF50476F8AE4D7C413367" italics="true" pageId="0" pageNumber="45">Sasa palmata</emphasis>
|
||
</taxonomicName>
|
||
, culture NBRC 115661.
|
||
</paragraph>
|
||
<caption id="AE287EF274BD0B1363A850152E7D6ACF" doi="10.3897/mycokeys.99.90117.figure8" httpUri="https://binary.pensoft.net/fig/892424" pageId="0" pageNumber="45" start="Figure 8" startId="F8">
|
||
<paragraph id="241842A748BF285FC41CBBA0DA5033FE" pageId="0" pageNumber="45">
|
||
<emphasis id="D05F18E35E2EDF32868BA6E99B4DE7E6" bold="true" pageId="0" pageNumber="45">Figure 8.</emphasis>
|
||
<taxonomicName id="E52E5C55F2F596BE953163AC5414CB4B" authorityName="Itagaki & Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis multiguttata" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="multiguttata">
|
||
<emphasis id="2A3602255BC260857D126A71EB47225D" italics="true" pageId="0" pageNumber="45">Neobelonopsis multiguttata</emphasis>
|
||
</taxonomicName>
|
||
(TNS-F-86402,
|
||
<typeStatus id="87B51DFFF7F2FF65D01D682738B5AA1C">holotype</typeStatus>
|
||
)
|
||
<emphasis id="96E4F170EB8D4F4EABDC60ED807A9525" bold="true" pageId="0" pageNumber="45">A</emphasis>
|
||
fresh apothecia on decaying culm of
|
||
<taxonomicName id="D6B69103A8643E489DB822B8220A10F8" authorityName="Makino & Shibata" authorityYear="1901" baseAuthorityName="Rupr." class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa kurilensis" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="kurilensis">
|
||
<emphasis id="77664A9EF626CA4F9F6D1F424EC079C6" italics="true" pageId="0" pageNumber="45">Sasa kurilensis</emphasis>
|
||
</taxonomicName>
|
||
<emphasis id="D62DB0E9866B48A081EDCF64B7A7FF87" bold="true" pageId="0" pageNumber="45">B</emphasis>
|
||
dried apothecia
|
||
<emphasis id="4D0269A5788D0207FB14F2A58E0366DF" bold="true" pageId="0" pageNumber="45">C</emphasis>
|
||
stromata with sparse subiculum
|
||
<emphasis id="6D05ABA9AF4A03962D2500CCEAE033E8" bold="true" pageId="0" pageNumber="45">D</emphasis>
|
||
vertical section of apothecium (in LA)
|
||
<emphasis id="F331681FA848BB4434E8A0645DF70A45" bold="true" pageId="0" pageNumber="45">E</emphasis>
|
||
ascospores (in CB/LA)
|
||
<emphasis id="60291B6D2A61A1DB5D6A4011303EE526" bold="true" pageId="0" pageNumber="45">F</emphasis>
|
||
asci with ascospores (in CB/LA)
|
||
<emphasis id="CD4212D592EDCF6DB5EFC2EDCE6C2DA5" bold="true" pageId="0" pageNumber="45">G</emphasis>
|
||
paraphyses (in CB/LA)
|
||
<emphasis id="2671CDA9A13CF8CB98419464F3A6A06B" bold="true" pageId="0" pageNumber="45">H</emphasis>
|
||
blue-stained apical pore of ascus (in
|
||
<normalizedToken id="D62CE6FFAFF342199D53D81FE688F26C" originalValue="Melzer’s">Melzer's</normalizedToken>
|
||
solution after 3% KOH pretreatment)
|
||
<emphasis id="7732C3CBD9B0B0BF5CA91DEBA1CB8EDF" bold="true" pageId="0" pageNumber="45">I</emphasis>
|
||
croziers at the base of asci (in CB/LA)
|
||
<emphasis id="E8C6A659A40CA4FF2CECD8116320798E" bold="true" pageId="0" pageNumber="45">J</emphasis>
|
||
texture of stroma (in LA)
|
||
<emphasis id="C84422B55AE2D41EE40C17B8476D5A9E" bold="true" pageId="0" pageNumber="45">K</emphasis>
|
||
subicular hyphae (in lactic acid)
|
||
<emphasis id="6B624B509C4766E92CBE477A8CB83862" bold="true" pageId="0" pageNumber="45">L</emphasis>
|
||
three months old colony on PDA
|
||
<emphasis id="13010CE24E20ECEB9CBC7491EA32C173" bold="true" pageId="0" pageNumber="45">M</emphasis>
|
||
conidia (in CB/LA)
|
||
<emphasis id="FACB81FCF2B7391B5F75F5BC020DC4DF" bold="true" pageId="0" pageNumber="45">N-R</emphasis>
|
||
conidiophores, arrows in
|
||
<emphasis id="9D798A191C7400E51EC279F7953E524A" bold="true" pageId="0" pageNumber="45">N</emphasis>
|
||
indicated conidiogenous cells (in CB/LA). Scale bars:
|
||
<quantity id="51EE5C675A4F3B39E54DC08B872D1CBA" metricMagnitude="-4" metricUnit="m" metricValue="5.0" unit="mm" value="0.5">0.5 mm</quantity>
|
||
(
|
||
<emphasis id="D595C49E09E1AD0D3C93FC0CB27A2F22" bold="true" pageId="0" pageNumber="45">A-C</emphasis>
|
||
); 50
|
||
<normalizedToken id="211BBC76CF6863AF1476CF3E41379D1F" originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis id="EAC4F36CB55181231CCCEF443FD71732" bold="true" pageId="0" pageNumber="45">D, K</emphasis>
|
||
); 20
|
||
<normalizedToken id="112F0890CFD9C8465BD6D67BB06F40EA" originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis id="CBECD3F4EDD95B8732BD78C7ECEEF03E" bold="true" pageId="0" pageNumber="45">N</emphasis>
|
||
), 10
|
||
<normalizedToken id="0A6F84C086976DB595593D9ABD8BA96D" originalValue="μm">μm</normalizedToken>
|
||
(
|
||
<emphasis id="AECDB0F86175D529C1ECB074D16AB175" bold="true" pageId="0" pageNumber="45">E-J, M, O-R</emphasis>
|
||
).
|
||
</paragraph>
|
||
</caption>
|
||
</subSubSection>
|
||
<subSubSection id="0DDA4A58DA797597C620B337BA5B40DC" pageId="0" pageNumber="45" type="notes">
|
||
<paragraph id="856133A554B504FE42E0C8296EB4F207" pageId="0" pageNumber="45">Notes.</paragraph>
|
||
<paragraph id="615B7554F8F4C31E4ED9F90A01D6A2BA" pageId="0" pageNumber="45">
|
||
<taxonomicName id="392AFAABF5DB9BEC60610A822753F9F1" authorityName="Itagaki & Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis multiguttata" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="multiguttata">
|
||
<emphasis id="C5780FCE7FA36F6E8BDB45D7A10CAB4C" italics="true" pageId="0" pageNumber="45">Neobelonopsis multiguttata</emphasis>
|
||
</taxonomicName>
|
||
has a wide host range, such as
|
||
<taxonomicName id="56B63ADA849477292820262BE768B34B" class="Monocotyledoneae" family="Poaceae" genus="Sasa" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Sasa" order="Graminales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="genus">
|
||
<emphasis id="47CCC60ADFCB62CC1E4DDBB18C167C7A" italics="true" pageId="0" pageNumber="45">Sasa</emphasis>
|
||
</taxonomicName>
|
||
spp.,
|
||
<taxonomicName id="C00DAD7A7DEAD167E059CA8B1D8E7341" class="Dicotyledoneae" family="Fagaceae" genus="Fagus" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Fagus crenata" order="Fagales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="crenata">
|
||
<emphasis id="2DD20BE46FA43328830EED1A97E98EC9" italics="true" pageId="0" pageNumber="45">Fagus crenata</emphasis>
|
||
</taxonomicName>
|
||
, and
|
||
<taxonomicName id="343E6AB222524BEBC1628EE74CD70C94" authorityName="Siebold & Zucc. ex Zabel" authorityYear="1885" baseAuthorityName="Thunb." class="Dicotyledoneae" family="Rosaceae" genus="Stephanandra" higherTaxonomySource="GBIF" kingdom="Plantae" lsidName="Stephanandra incisa" order="Rosales" pageId="0" pageNumber="45" phylum="Angiospermae" rank="species" species="incisa">
|
||
<emphasis id="73D8FA030F6D0BEC06868C6F5A203598" italics="true" pageId="0" pageNumber="45">Stephanandra incisa</emphasis>
|
||
</taxonomicName>
|
||
, and occurs on various substrates, such as culms, branches, and cupules.
|
||
<taxonomicName id="CEF6FA74D3C6F6085D0D8353AFEBA631" authorityName="Itagaki & Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis multiguttata" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="multiguttata">
|
||
<emphasis id="3C50B0BC3E5893F1A7F8A8A1077BAAAA" italics="true" pageId="0" pageNumber="45">Neobelonopsis multiguttata</emphasis>
|
||
</taxonomicName>
|
||
was found in spring and its morphology overlaps with
|
||
<taxonomicName id="59323C1F02A46FE7002E676DF6A445BB" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. bicolor" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="bicolor">
|
||
<emphasis id="7170E5E81162410B25D74F1CBFFFFA49" italics="true" pageId="0" pageNumber="45">N. bicolor</emphasis>
|
||
</taxonomicName>
|
||
in the dimensions of apothecia and paraphyses. However, the ITS sequence similarity with
|
||
<taxonomicName id="0523F918ECFE04A90EBD33EE2EF8F4D4" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. acutata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="acutata">
|
||
<emphasis id="AD5E98D16DE09EE9A427BF2D5EE8F4C0" italics="true" pageId="0" pageNumber="45">N. acutata</emphasis>
|
||
</taxonomicName>
|
||
is only 93.8%. Further, the two species form phylogenetically distinct clades (Fig.
|
||
<figureCitation id="B5B8DB5D6C6275560F2B5BCAE9AA3E32" captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Maximum likelihood tree inferred from ITS-LSU-RPB 1 concatenate sequences used in Tanney and Seifert (2020), together with sequences from additional taxa of Neobelonopsis and two new species of Trichobelonium. Significant branch supported by SH-aLRT (> 80 %) / Ultrafast BS (> 95 %) / BPP (> 0.95) are indicated. Type species are in red. Collection numbers are shown at the beginning of the species name (type strains are in boldface). The tree is rooted with Cadophora dextrinospora and C. fastigiata." figureDoi="10.3897/mycokeys.99.90117.figure1" httpUri="https://binary.pensoft.net/fig/892417" pageId="0" pageNumber="45">1</figureCitation>
|
||
). The conidiophores of
|
||
<taxonomicName id="A9DAC57BEA3C3AB204AB78B3055D401F" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. multiguttata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="multiguttata">
|
||
<emphasis id="0A05E930BBC49DDE4AC9BBB852413238" italics="true" pageId="0" pageNumber="45">N. multiguttata</emphasis>
|
||
</taxonomicName>
|
||
on CMA are discrete (Figs
|
||
<figureCitation id="90F03412D8E1FBFF7C6699D99B79F7D5" captionStart="Figure 8" captionStartId="F8" captionText="Figure 8. Neobelonopsis multiguttata (TNS-F- 86402, holotype) A fresh apothecia on decaying culm of Sasa kurilensis B dried apothecia C stromata with sparse subiculum D vertical section of apothecium (in LA) E ascospores (in CB / LA) F asci with ascospores (in CB / LA) G paraphyses (in CB / LA) H blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) I croziers at the base of asci (in CB / LA) J texture of stroma (in LA) K subicular hyphae (in lactic acid) L three months old colony on PDA M conidia (in CB / LA) N-R conidiophores, arrows in N indicated conidiogenous cells (in CB / LA). Scale bars: 0.5 mm (A-C); 50 μm (D, K); 20 μm (N), 10 μm (E-J, M, O-R)." figureDoi="10.3897/mycokeys.99.90117.figure8" httpUri="https://binary.pensoft.net/fig/892424" pageId="0" pageNumber="45">8N-R</figureCitation>
|
||
,
|
||
<figureCitation id="0422FE701EC2BB257980037227057AD9" captionStart="Figure 14" captionStartId="F14" captionText="Figure 14. Asexual morph and hyphal structures of the Neobelonopsis and Trichobelonium species A conidiophores of N. acutata B monilioid hyphae of N. bicolor C conidiophores with conidia of N. cinnabarina D conidiophores with conidia of N. didymospora E conidiophores with conidia of N. microspora F conidiophores with conidia of N. multiguttata G conidiophores with conidia of N. obtusa H conidiophores with conidia of T. albobarbatum I hyphal mass with melanized appressorium-like structure of T. miscanthi. Scale bars: 10 μm." figureDoi="10.3897/mycokeys.99.90117.figure14" httpUri="https://binary.pensoft.net/fig/892430" pageId="0" pageNumber="45">14F</figureCitation>
|
||
), rather than aggregated as in
|
||
<taxonomicName id="D80CF6023219BFE4DCCC3E9E64AE03FE" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. acutata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="acutata">
|
||
<emphasis id="587F1125D24E6619157DA340785149B0" italics="true" pageId="0" pageNumber="45">N. acutata</emphasis>
|
||
</taxonomicName>
|
||
(Fig.
|
||
<figureCitation id="2503C4FC2E550747E20D4FABA35FD5FE" captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Neobelonopsis acutata (TNS-F- 86648, holotype) A fresh apothecia on the decaying culm of Miscanthus sinensis B dried apothecia C stromata with sparse subiculum D one month old colony on PDA E vertical section of the apothecium (in LA) F refractive vacuoles in fresh marginal cells (in water) G asci with ascospores (in CB / LA) H paraphyses (in CB / LA) I paraphyses with a long refractive vacuole (in water) J blue-stained apical pore of ascus (in Melzer's solution after 3 % KOH pretreatment) K croziers at the base of ascus (in CB / LA) L ascospores (in CB / LA) M conidia (in water) N, O conidiophores (in water) P bulbile (in CB / LA) Q subicular hyphae (in CB / LA). Scale bars: 1 mm (A, B); 0.2 mm (C); 25 μm (E, Q); 10 μm (F-P)." figureDoi="10.3897/mycokeys.99.90117.figure3" httpUri="https://binary.pensoft.net/fig/892419" pageId="0" pageNumber="45">3N, O</figureCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="5CD7777002814E230776F3395803E08F" pageId="0" pageNumber="45">
|
||
Based on a BLAST search for the ITS sequences of
|
||
<taxonomicName id="DD06912111E7500225836E7E191B755D" authorityName="Itagaki & Hosoya" authorityYear="2023" family="Mollisiaceae" genus="Neobelonopsis" kingdom="Fungi" lsidName="Neobelonopsis multiguttata" order="Helotiales" pageId="0" pageNumber="45" rank="species" species="multiguttata">
|
||
<emphasis id="466E1B977E8C715C95755D91585EA30E" italics="true" pageId="0" pageNumber="45">Neobelonopsis multiguttata</emphasis>
|
||
</taxonomicName>
|
||
in the GenBank database, the closest hit was
|
||
<taxonomicName id="BC549F5EF3145722AD140890A75DDD83" class="Leotiomycetes" family="Mollisiaceae" genus="Ascomycota" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Ascomycota" pageId="0" pageNumber="45" phylum="Ascomycota" rank="genus">
|
||
<emphasis id="41D899854511FE6660B8F69290834170" italics="true" pageId="0" pageNumber="45">Ascomycota</emphasis>
|
||
</taxonomicName>
|
||
sp. (MK842071), isolated from the needles and roots of pine trees in South Korea [Identities=531/531 (100%), no gaps]. The endophytic isolate was recognized as
|
||
<taxonomicName id="699BA2A811228FEEE00F43D2A309F8CC" authorityName="P.A.Karsten" authorityYear="1871" baseAuthorityName="E.M.Fries" class="Ascomycetes" family="Discomycetes" genus="Mollisia" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="Mollisia" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="genus">
|
||
<emphasis id="C69D68926DC8A00CF9543B4700697442" italics="true" pageId="0" pageNumber="45">Mollisia</emphasis>
|
||
</taxonomicName>
|
||
sp. by
|
||
<bibRefCitation id="94D5E961B60FD6D110FBBA8E6C5E2364" DOI="https://doi.org/10.3390/f12030302" author="Rim, SO" journalOrPublisher="Forests" pageId="0" pageNumber="45" pagination=": 302" refId="B43" refString="Rim, SO, Roy, M, Jeon, J, Montecillo, JAV, Park, S-C, Bae, H, 2021. Diversity and Communities of Fungal Endophytes from Four Pinus Species in Korea. Forests 12 (3): 302, DOI: https://doi.org/10.3390/f12030302" title="Diversity and Communities of Fungal Endophytes from Four Pinus Species in Korea." url="https://doi.org/10.3390/f12030302" volume="12" year="2021">Rim et al. (2021)</bibRefCitation>
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. This result suggests that
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<taxonomicName id="93653A1B6898D72513E142E99BF9E595" class="Leotiomycetes" family="Mollisiaceae" higherTaxonomySource="GBIF" kingdom="Fungi" lsidName="N. multiguttata" order="Helotiales" pageId="0" pageNumber="45" phylum="Ascomycota" rank="species" species="multiguttata">
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<emphasis id="60BA21B3B80D0F8BAFDF1C71C0145FB8" italics="true" pageId="0" pageNumber="45">N. multiguttata</emphasis>
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</taxonomicName>
|
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has an endophytic phase as part of its life cycle.
|
||
</paragraph>
|
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</subSubSection>
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</treatment>
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</document> |