209 lines
14 KiB
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209 lines
14 KiB
XML
<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.60.38040" ID-GBIF-Dataset="65d938c8-cdd5-47f8-803b-bfcd4920bd64" ID-PMC="PMC6879665" ID-Pensoft-Pub="1314-4049-60-69" ID-Pensoft-UUID="858D1D355F5851868AFCE4A14F1394B8" ID-PubMed="31798310" ModsDocID="1314-4049-60-69" checkinTime="1574358804276" checkinUser="pensoft" docAuthor="Wei, De-Ping, Wanasinghe, Dhanushka N., Hyde, Kevin D., Mortimer, Peter E., Xu, Jianchu, Xiao, Yuan-Pin, Bhunjun, Chitrabhanu S. & To-anun, Chaiwat" docDate="2019" docId="DC1DBEE168F253E1A4BA5AF04F7CCBDE" docLanguage="en" docName="MycoKeys 60: 69-92" docOrigin="MycoKeys 60" docSource="http://dx.doi.org/10.3897/mycokeys.60.38040" docTitle="Simplicillium formicae D. P. Wei & K. D. Hyde, sp. nov." docType="treatment" docVersion="4" id="858D1D355F5851868AFCE4A14F1394B8" lastPageNumber="69" masterDocId="858D1D355F5851868AFCE4A14F1394B8" masterDocTitle="The genus Simplicillium" masterLastPageNumber="92" masterPageNumber="69" pageNumber="69" updateTime="1668136349009" updateUser="ExternalLinkService">
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<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
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<mods:titleInfo>
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<mods:title>The genus Simplicillium</mods:title>
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</mods:titleInfo>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Wei, De-Ping</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Wanasinghe, Dhanushka N.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Hyde, Kevin D.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Mortimer, Peter E.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xu, Jianchu</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Xiao, Yuan-Pin</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>Bhunjun, Chitrabhanu S.</mods:namePart>
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</mods:name>
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<mods:name type="personal">
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<mods:role>
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<mods:roleTerm>Author</mods:roleTerm>
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</mods:role>
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<mods:namePart>To-anun, Chaiwat</mods:namePart>
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</mods:name>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:relatedItem type="host">
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<mods:titleInfo>
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<mods:title>MycoKeys</mods:title>
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</mods:titleInfo>
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<mods:part>
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<mods:date>2019</mods:date>
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<mods:detail type="volume">
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<mods:number>60</mods:number>
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</mods:detail>
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<mods:extent unit="page">
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<mods:start>69</mods:start>
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<mods:end>92</mods:end>
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</mods:extent>
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</mods:part>
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</mods:relatedItem>
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<mods:location>
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<mods:url>http://dx.doi.org/10.3897/mycokeys.60.38040</mods:url>
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</mods:location>
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<mods:classification>journal article</mods:classification>
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<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.60.38040</mods:identifier>
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<mods:identifier type="Pensoft-Pub">1314-4049-60-69</mods:identifier>
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<mods:identifier type="Pensoft-UUID">858D1D355F5851868AFCE4A14F1394B8</mods:identifier>
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<treatment ID-GBIF-Taxon="160511187" LSID="urn:lsid:plazi:treatment:DC1DBEE168F253E1A4BA5AF04F7CCBDE" httpUri="http://treatment.plazi.org/id/DC1DBEE168F253E1A4BA5AF04F7CCBDE" lastPageNumber="69" pageId="0" pageNumber="69">
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<subSubSection pageId="0" pageNumber="69" type="nomenclature">
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<paragraph pageId="0" pageNumber="69">
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<taxonomicName LSID="DC1DBEE1-68F2-53E1-A4BA-5AF04F7CCBDE" authority="D. P. Wei & K. D. Hyde" class="Sordariomycetes" family="Cordycipitaceae" genus="Simplicillium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Simplicillium formicae" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="formicae">Simplicillium formicae D.P. Wei & K.D. Hyde</taxonomicName>
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<taxonomicNameLabel pageId="0" pageNumber="69">sp. nov.</taxonomicNameLabel>
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<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Simplicillium formicae (from HKAS 102459, holotype) a superficial hyphae associated with the ant host b-e flask-shaped synnemata f-k phialides bearing conidia l-p conidia. Scale bars: 1000 µm (a); 500 µm (b); 100 µm (d); 30 µm (e, f); 15 µm (j, k); 10 µm (l-p) (e stained with cotton blue solution)." figureDoi="10.3897/mycokeys.60.38040.figure3" httpUri="https://binary.pensoft.net/fig/357983" pageId="0" pageNumber="69" tableDoi="10.3897/mycokeys.60.38040.figure3">Figure 3</figureCitation>
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,
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<figureCitation captionStart="Figure 4" captionStartId="F4" captionText="Figure 4. Simplicillium formicae (MFLUCC 18 - 1379, ex-type living culture) a upper and reverse view of cultures on PDA after 30 days e-g phialides indicated with black arrow c, d, h-j conidial mass on the tip of phialides k-m conidia. Scale bars: 10 µm (c, d, f, g); 20 µm (e); 3 µm (h-j); 1 µm (k-m) (e-j stained with cotton blue solution)." figureDoi="10.3897/mycokeys.60.38040.figure4" httpUri="https://binary.pensoft.net/fig/357984" pageId="0" pageNumber="69" tableDoi="10.3897/mycokeys.60.38040.figure4">4</figureCitation>
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="69" type="etymology">
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<paragraph pageId="0" pageNumber="69">Etymology.</paragraph>
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<paragraph pageId="0" pageNumber="69">
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the epithet refers to its
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<normalizedToken originalValue="host–ant">host-ant</normalizedToken>
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.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="69" type="holotype">
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<paragraph pageId="0" pageNumber="69">Holotype.</paragraph>
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<paragraph pageId="0" pageNumber="69">HKAS 102459; living culture: MFLUCC 18-1379.</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="69" type="description">
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<paragraph pageId="0" pageNumber="69">Description.</paragraph>
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<paragraph pageId="0" pageNumber="69">
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Parasitic on ant (
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<taxonomicName class="Insecta" family="Formicidae" genus="Formicidae" higherTaxonomySource="GBIF" kingdom="Animalia" lsidName="Formicidae" order="Hymenoptera" pageId="0" pageNumber="69" phylum="Arthropoda" rank="genus">Formicidae</taxonomicName>
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).
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<emphasis bold="true" italics="true" pageId="0" pageNumber="69">Asexual morph</emphasis>
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: Hyphomycetous.
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<emphasis italics="true" pageId="0" pageNumber="69">Mycelium</emphasis>
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rarely septate, hyaline, smooth-walled, covering the whole body of the ant host.
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<taxonomicName genus="Synnemata" lsidName="Synnemata" pageId="0" pageNumber="69" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="69">Synnemata</emphasis>
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</taxonomicName>
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250-350
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<normalizedToken originalValue="×">x</normalizedToken>
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65-100 (xˉ = 300
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<normalizedToken originalValue="×">x</normalizedToken>
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86, n = 10)
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, forming at the head region of ant host in circular arrangement, flask-shaped, hyaline to yellowish, composed of dense hyphae, somehow curved.
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<emphasis italics="true" pageId="0" pageNumber="69">Phialides</emphasis>
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25-100
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<normalizedToken originalValue="×">x</normalizedToken>
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0.5-1.5 (xˉ = 49
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<normalizedToken originalValue="×">x</normalizedToken>
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1.1, n = 20)
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, arising from procumbent hyphae or synnemata, blastic, enteroblastic, phialidic, monophialidic, discrete, terminal, unbranched, solitary, aseptate, hyaline, smooth-walled, slender, occasionally a swollen node present.
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<emphasis italics="true" pageId="0" pageNumber="69">Conidia</emphasis>
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2-3.5
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-2.5 (xˉ = 2.6
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<normalizedToken originalValue="×">x</normalizedToken>
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2, n = 30)
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, globose to ellipsoidal, hyaline, one-celled, smooth-walled, round at both ends, adhering in slimy head on the tip of phialides.
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<emphasis bold="true" italics="true" pageId="0" pageNumber="69">Sexual morph</emphasis>
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: Undetermined.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="69" type="description">
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<paragraph pageId="0" pageNumber="69">Culture characteristics.</paragraph>
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<paragraph pageId="0" pageNumber="69">
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The colonies were rapid-growing on PDA medium, reaching a diameter of 2.5-3 (xˉ = 2.6, n = 9) cm, in 13 days at 22 °C, entire margin, circular, velvety and white from above, with radial crack and primrose-yellow on reverse.
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<emphasis italics="true" pageId="0" pageNumber="69">In vitro</emphasis>
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,
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<taxonomicName genus="Synnemata" lsidName="Synnemata" pageId="0" pageNumber="69" rank="genus">
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<emphasis italics="true" pageId="0" pageNumber="69">Synnemata</emphasis>
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</taxonomicName>
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absent.
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<emphasis italics="true" pageId="0" pageNumber="69">Phialides</emphasis>
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25-75
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<normalizedToken originalValue="×">x</normalizedToken>
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0.4-0.6 (xˉ = 50
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<normalizedToken originalValue="×">x</normalizedToken>
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0.55, n = 10)
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, arising from procumbent hyphae, blastic, enteroblastic, phialidic, discrete, terminal, unbranched, solitary, aseptate, hyaline, smooth-walled, relatively slender and long.
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<emphasis italics="true" pageId="0" pageNumber="69">Conidia</emphasis>
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1.5-3
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<normalizedToken originalValue="×">x</normalizedToken>
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1.5-2.5 (xˉ = 2.3
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<normalizedToken originalValue="×">x</normalizedToken>
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1.7, n = 100)
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<normalizedToken originalValue="µm">µm</normalizedToken>
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, hyaline, globose to ellipsoidal, aseptate, smooth-walled, slightly guttulate, adhering in slimy head on the tip of phialides.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="69" type="materials_examined">
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<paragraph pageId="0" pageNumber="69">Material examined.</paragraph>
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<paragraph pageId="0" pageNumber="69">
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THAILAND, Chiang Mai Province, Mushroom Research Centre, on an adult ant, 1 April 2018,
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<emphasis italics="true" pageId="0" pageNumber="69">Deping Wei</emphasis>
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, MRC18040102 (
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<emphasis bold="true" italics="true" pageId="0" pageNumber="69">holotype</emphasis>
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: HKAS 102459;
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<emphasis bold="true" italics="true" pageId="0" pageNumber="69">ex-type living culture</emphasis>
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: MFLUCC 18-1379). Sequences generated from this strain have been deposited in GenBank with accession numbers: SSU = MK765046, LSU = MK766512, ITS = MK766511, TEF = MK926451, RPB1 = MK882623.
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</paragraph>
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</subSubSection>
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<subSubSection pageId="0" pageNumber="69" type="notes">
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<paragraph pageId="0" pageNumber="69">Note.</paragraph>
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<paragraph pageId="0" pageNumber="69">
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Isolate MFLUCC 18-1379 has a close phylogenetic relationship with
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<taxonomicName class="Sordariomycetes" family="Cordycipitaceae" genus="Simplicillium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Simplicillium obclavatum" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="obclavatum">
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<emphasis italics="true" pageId="0" pageNumber="69">Simplicillium obclavatum</emphasis>
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</taxonomicName>
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, based on ITS sequence analysis. The new isolate is similar to
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<taxonomicName class="Sordariomycetes" family="Cordycipitaceae" genus="Simplicillium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Simplicillium obclavatum" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="obclavatum">
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<emphasis italics="true" pageId="0" pageNumber="69">Simplicillium obclavatum</emphasis>
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</taxonomicName>
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in terms of shape and dimensions of the conidia with slender phialides tapering towards the apex. However, they have a different conidial arrangement, by
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<taxonomicName class="Sordariomycetes" family="Cordycipitaceae" genus="Simplicillium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Simplicillium obclavatum" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="obclavatum">
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<emphasis italics="true" pageId="0" pageNumber="69">Simplicillium obclavatum</emphasis>
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</taxonomicName>
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having short-imbricate chains, whereas the new fungus has subglobose to globose head. There are numerous synnemata in a circular arrangement which can be observed in our isolate and those are absent in
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<taxonomicName class="Sordariomycetes" family="Cordycipitaceae" genus="Simplicillium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Simplicillium obclavatum" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="obclavatum">
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<emphasis italics="true" pageId="0" pageNumber="69">Simplicillium obclavatum</emphasis>
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</taxonomicName>
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. The comparisons of ITS sequences between our isolate MFLUCC 18-1379 and ex-type strain of
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<taxonomicName class="Sordariomycetes" family="Cordycipitaceae" genus="Simplicillium" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Simplicillium obclavatum" order="Hypocreales" pageId="0" pageNumber="69" phylum="Ascomycota" rank="species" species="obclavatum">
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<emphasis italics="true" pageId="0" pageNumber="69">Simplicillium obclavatum</emphasis>
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</taxonomicName>
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(CBS 311.74) show 23 bp differences within 550 bp (4.2%). Thereby, we identify our isolates as a new species according to
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<bibRefCitation DOI="https://doi.org/10.5943/mycosphere/7/11/4" author="Jeewon, R" journalOrPublisher="Mycosphere" pageId="0" pageNumber="69" pagination="1669 - 1677" refId="B24" refString="Jeewon, R, Hyde, KD, 2016. Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669 - 1677" title="Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities." url="https://doi.org/10.5943/mycosphere/7/11/4" volume="7" year="2016">Jeewon and Hyde (2016)</bibRefCitation>
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.
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</paragraph>
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</subSubSection>
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</treatment>
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</document> |