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<document id="7102D9BD09822E0D4551C805B8A1AF00" ID-CLB-Dataset="55799" ID-DOI="10.11646/phytotaxa.544.1.3" ID-GBIF-Dataset="57a54d17-7281-4eae-92b9-5d57b992b9d2" ID-ISSN="1179-3163" ID-Zenodo-Dep="6501342" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1651146497482" checkinUser="plazi" docAuthor="Lu, Qing-Tao, Zhang, Jing-Yi, Sun, Ya-Ru, Tang, Xia, Lu, Yong-Zhong &amp; Zhang, Zhen" docDate="2022" docId="03D4878F330EFFCEFF03FAF423A0FD79" docLanguage="en" docName="phytotaxa.544.1.3.pdf" docOrigin="Phytotaxa 544 (1)" docStyle="DocumentStyle:96748F8F1B6C902996E134952A3A36B9.7:Phytotaxa.2014-.journal_article" docStyleId="96748F8F1B6C902996E134952A3A36B9" docStyleName="Phytotaxa.2014-.journal_article" docStyleVersion="7" docTitle="Diaporthe caryae C. M. Tian &amp; Q. Yang, MycoKeys" docType="treatment" docVersion="8" lastPageNumber="48" masterDocId="FFEDFFF73307FFC5FF8BFFEA2152FFC9" masterDocTitle="Diaporthe orixae sp. nov., an endophytic species isolated from Orixa japonica in southern China" masterLastPageNumber="51" masterPageNumber="37" pageNumber="46" updateTime="1699329389142" updateUser="ExternalLinkService" zenodo-license-figures="UNSPECIFIED" zenodo-license-treatments="UNSPECIFIED">
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<mods:title id="3F5FFF10C0DC923E84C91C4E41953886">Diaporthe orixae sp. nov., an endophytic species isolated from Orixa japonica in southern China</mods:title>
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<mods:namePart id="0E52F39E90AAAA40BB1C9EEE5514E9B9">Lu, Qing-Tao</mods:namePart>
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<mods:affiliation id="AB766420214EC4469F4089970863C1FF">School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, P. R. China. &amp; Guizhou Academy of Testing and Analysis, Guiyang 550000, P. R. China. &amp; School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, P. R. China. &amp; 1341776389 @ qq. com; https: // orcid. org / 0000 - 0002 - 1768 - 0271</mods:affiliation>
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<mods:namePart id="58F0597B1859DB6A6D46DC111EC5FBBA">Zhang, Jing-Yi</mods:namePart>
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<mods:affiliation id="C4AC840D031ED3006653658370D03718">School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, P. R. China. &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. &amp; zjingyi 127 @ gmail. com; https: // orcid. org / 0000 - 0003 - 0606 - 6169</mods:affiliation>
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<mods:namePart id="FA740F3366328ABF9AAE4F0475E28645">Sun, Ya-Ru</mods:namePart>
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<mods:affiliation id="EC7D103979C6D5580C7132F5BD605911">Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. &amp; yarusun 5 @ gmail. com; https: // orcid. org / 0000 - 0001 - 5549 - 1028</mods:affiliation>
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<mods:namePart id="E7EC0A763C6794A1709EC414DEB477EF">Tang, Xia</mods:namePart>
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<mods:affiliation id="D9C562CB008DA9CCD7C577B160998896">School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, P. R. China. &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand. &amp; bella 19580412 @ gmail. com; https: // orcid. org / 0000 - 0003 - 2705 - 604 X</mods:affiliation>
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<mods:namePart id="60DF020C16F2705E35DC626E9D486982">Lu, Yong-Zhong</mods:namePart>
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<mods:affiliation id="C033F9D1538C4C696EA7E575E6F88664">School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550003, P. R. China. &amp; yzlu @ git. edu. cn; https: // orcid. org / 0000 - 0002 - 1033 - 5782</mods:affiliation>
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<mods:namePart id="949515264FCD61D08597E7BA5B6026A7">Zhang, Zhen</mods:namePart>
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<mods:affiliation id="1A0BA5B74CA7E21138C38EA3788D88F4">School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, P. R. China. &amp; Guizhou Academy of Testing and Analysis, Guiyang 550000, P. R. China. &amp; zhangzhen @ git. edu. cn; https: // orcid. org / 0000 - 0001 - 6753 - 2288</mods:affiliation>
<mods:nameIdentifier id="7F2B762732197ECD1EF86D6F38D6F3D6" type="email">zhangzhen@git.edu.cn</mods:nameIdentifier>
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<mods:title id="B7677DEE2EAB24EA90B64F9B84DE6CBE">Phytotaxa</mods:title>
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<mods:date id="20F42FACACA4DB9571E48AB8F7F9AF67">2022</mods:date>
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<mods:number id="2676E426894771EB5802AFE58E75B1BF">2022-04-27</mods:number>
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<mods:number id="D5F7BD346A330EE9A893ADC8343CDB7F">544</mods:number>
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<mods:identifier id="4A97C977A54988FDEEEB26B614B0E29A" type="DOI">10.11646/phytotaxa.544.1.3</mods:identifier>
<mods:identifier id="3F4FECF08A8CE1EC08C0DFCA21999AAE" type="GBIF-Dataset">57a54d17-7281-4eae-92b9-5d57b992b9d2</mods:identifier>
<mods:identifier id="490373CE330CB33ECE51D3FFBBA8569C" type="ISSN">1179-3163</mods:identifier>
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<treatment id="03D4878F330EFFCEFF03FAF423A0FD79" ID-DOI="http://doi.org/10.5281/zenodo.6504883" ID-GBIF-Taxon="194873536" ID-Zenodo-Dep="6504883" LSID="urn:lsid:plazi:treatment:03D4878F330EFFCEFF03FAF423A0FD79" httpUri="http://treatment.plazi.org/id/03D4878F330EFFCEFF03FAF423A0FD79" lastPageId="11" lastPageNumber="48" pageId="9" pageNumber="46">
<subSubSection id="C3676512330EFFCCFF03FAF42261FA95" pageId="9" pageNumber="46" type="nomenclature">
<paragraph id="8BC23699330EFFCCFF03FAF4257BFAF1" blockId="9.[136,1065,1310,1337]" box="[136,1065,1310,1337]" pageId="9" pageNumber="46">
<heading id="D08A81F5330EFFCCFF03FAF4257BFAF1" box="[136,1065,1310,1337]" fontSize="11" level="2" pageId="9" pageNumber="46" reason="3">
<taxonomicName id="4C7D4D1A330EFFCCFF03FAF42383FAF0" ID-CoL="35CR9" authority="C. M. Tian &amp; Q. Yang, MycoKeys" authorityName="C. M. Tian &amp; Q. Yang, MycoKeys" box="[136,721,1310,1337]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="9" pageNumber="46" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330EFFCCFF03FAF42002FAF0" bold="true" box="[136,336,1310,1337]" italics="true" pageId="9" pageNumber="46">Diaporthe caryae</emphasis>
C.M. Tian &amp; Q. Yang, MycoKeys
</taxonomicName>
39, 97149 (2018)
<figureCitation id="13462A1C330EFFCCFC24FAF4257BFAF1" box="[943,1065,1310,1336]" captionStart="FIGURE 3" captionStartId="10.[136,229,1908,1930]" captionTargetBox="[151,1436,187,1880]" captionTargetId="figure-24@10.[151,1436,187,1881]" captionTargetPageId="10" captionText="FIGURE 3. Diaporthe caryae (GZAAS 210393, new host record) a, b Colonies on MEA. c Immersed conidiomata. d Peridium. e Alpha and beta conidia fi Conidiogenous cells. j, k Alpha conidia. ln Beta conidia. Scale bars: d, e, ln = 10 µm, fk = 5 µm." figureDoi="http://doi.org/10.5281/zenodo.6501350" httpUri="https://zenodo.org/record/6501350/files/figure.png" pageId="9" pageNumber="46">FIGURE 3</figureCitation>
</heading>
</paragraph>
<paragraph id="8BC23699330EFFCCFF03FAA82261FA95" blockId="9.[136,819,1346,1372]" box="[136,819,1346,1372]" pageId="9" pageNumber="46">
<emphasis id="B909EA8B330EFFCCFF03FAA820FBFA95" box="[136,425,1346,1372]" italics="true" pageId="9" pageNumber="46">Index Fungorum number:</emphasis>
IF824706,
<emphasis id="B909EA8B330EFFCCFDA0FAA823FEFA95" box="[555,684,1346,1372]" italics="true" pageId="9" pageNumber="46">MycoBank:</emphasis>
MB824706
</paragraph>
</subSubSection>
<subSubSection id="C3676512330EFFCEFF03FA6023A0FD79" lastPageId="11" lastPageNumber="48" pageId="9" pageNumber="46" type="etymology">
<paragraph id="8BC23699330EFFCCFF03FA6022EFFA6C" blockId="9.[136,1452,1418,2056]" box="[136,957,1418,1445]" pageId="9" pageNumber="46">
Etymology: Named after the host genus on which it was collected,
<taxonomicName id="4C7D4D1A330EFFCCFCFCFA6122EFFA6C" box="[887,957,1419,1445]" class="Magnoliopsida" family="Juglandaceae" genus="Carya" kingdom="Plantae" order="Fagales" pageId="9" pageNumber="46" phylum="Tracheophyta" rank="genus">
<emphasis id="B909EA8B330EFFCCFCFCFA6122EFFA6C" box="[887,957,1419,1445]" italics="true" pageId="9" pageNumber="46">Carya</emphasis>
</taxonomicName>
</paragraph>
<paragraph id="8BC23699330EFFCCFF36FA442097FA01" blockId="9.[136,1452,1418,2056]" box="[189,453,1454,1480]" pageId="9" pageNumber="46">
<materialsCitation id="3B153CC4330EFFCCFF36FA442097FA01" ID-GBIF-Occurrence="3757889303" box="[189,453,1454,1480]" pageId="9" pageNumber="46" specimenCode="BJFC-S1476" specimenCount="1" typeStatus="holotype">
<typeStatus id="54C6883B330EFFCCFF36FA442078FA01" box="[189,298,1454,1480]" pageId="9" pageNumber="46" type="holotype">Holotype</typeStatus>
:
<specimenCode id="DBDB9EE2330EFFCCFEBFFA442097FA01" box="[308,453,1454,1480]" pageId="9" pageNumber="46">BJFC-S1476</specimenCode>
</materialsCitation>
</paragraph>
<paragraph id="8BC23699330EFFCCFF36FA3823C7F90D" blockId="9.[136,1452,1418,2056]" pageId="9" pageNumber="46">
Endophytic in the stem of
<taxonomicName id="4C7D4D1A330EFFCCFD8DFA3923E5FA25" box="[518,695,1491,1517]" class="Magnoliopsida" family="Rutaceae" genus="Orixa" kingdom="Plantae" order="Sapindales" pageId="9" pageNumber="46" phylum="Tracheophyta" rank="species" species="japonica">
<emphasis id="B909EA8B330EFFCCFD8DFA3923E5FA25" box="[518,695,1491,1517]" italics="true" pageId="9" pageNumber="46">Orixa japonica</emphasis>
</taxonomicName>
.
<emphasis id="B909EA8B330EFFCCFD47FA3822D1FA25" bold="true" box="[716,899,1490,1516]" pageId="9" pageNumber="46">Sexual morph:</emphasis>
Undetermined.
<emphasis id="B909EA8B330EFFCCFBCEFA382459FA25" bold="true" box="[1093,1291,1490,1516]" pageId="9" pageNumber="46">Asexual morph:</emphasis>
<emphasis id="B909EA8B330EFFCCFA92FA3824F9FA25" box="[1305,1451,1490,1516]" italics="true" pageId="9" pageNumber="46">Conidiomata</emphasis>
pycnidial, sporulating poorly on MEA, immersed in the medium, aggregated, globose, multilocular, black, erumpent.
<emphasis id="B909EA8B330EFFCCFF03F9F0207FF9FD" box="[136,301,1562,1588]" italics="true" pageId="9" pageNumber="46">Conidiophores</emphasis>
hyaline, smooth, 13-septate, branched, densely aggregated, cylindrical, straight to sinuous, 6.016 (21.0) × 1.73.0 μm (
<emphasis id="B909EA8B330EFFCCFE05F9D520C8F991" box="[398,410,1599,1624]" italics="true" pageId="9" pageNumber="46"></emphasis>
= 11.5 × 2.5 μm, n = 15).
<emphasis id="B909EA8B330EFFCCFD44F9D4223DF991" box="[719,879,1598,1624]" italics="true" pageId="9" pageNumber="46">Alpha conidia</emphasis>
3.56.5 × 1.52.5 μm (
<emphasis id="B909EA8B330EFFCCFBF4F9D525D9F991" box="[1151,1163,1599,1624]" italics="true" pageId="9" pageNumber="46"></emphasis>
= 5.3 × 2.2 μm, n = 30), aseptate, hyaline, smooth, guttulate, ellipsoidal or fusiform, tapering towards ends or obtuse at both ends.
<emphasis id="B909EA8B330EFFCCFA95F98924F9F9B5" box="[1310,1451,1634,1660]" italics="true" pageId="9" pageNumber="46">Beta conidia</emphasis>
hyaline, aseptate, filiform, straight or hamate, slightly tapering towards both ends, eguttulate, base subtruncate, 15.0 24.5 × 1.02.0 μm (
<emphasis id="B909EA8B330EFFCCFEECF9412021F90D" box="[359,371,1707,1732]" italics="true" pageId="9" pageNumber="46"></emphasis>
= 20.7 × 1.4 μm, n = 30).
</paragraph>
<paragraph id="8BC23699330EFFCCFF36F92425ACF8C5" blockId="9.[136,1452,1418,2056]" pageId="9" pageNumber="46">
<emphasis id="B909EA8B330EFFCCFF36F92420CEF921" box="[189,412,1742,1768]" italics="true" pageId="9" pageNumber="46">Culture characters:</emphasis>
Cultures incubated on MEA at 25 °C. Colony entirely white at surface, reverse with pale brown pigmentation, white, fluffy aerial mycelium, the sporulation distributed in the media randomly.
</paragraph>
<paragraph id="8BC23699330EFFCCFF36F8FD2580F8F8" blockId="9.[136,1452,1418,2056]" box="[189,1234,1814,1841]" pageId="9" pageNumber="46">
<emphasis id="B909EA8B330EFFCCFF36F8FD20CDF8F9" box="[189,415,1814,1840]" italics="true" pageId="9" pageNumber="46">Known distribution:</emphasis>
<collectingCountry id="F36A7609330EFFCCFE2CF8FC20ACF8F9" box="[423,510,1814,1840]" name="China" pageId="9" pageNumber="46">CHINA</collectingCountry>
(
<bibRefCitation id="EFEC4B68330EFFCCFD87F8FC239BF8F9" author="Yang, Q. &amp; Fan, X. L. &amp; Guarnaccia, V. &amp; Tian, C. M." box="[524,713,1814,1841]" pageId="9" pageNumber="46" pagination="97 - 149" refId="ref10010" refString="Yang, Q., Fan, X. L., Guarnaccia, V. &amp; Tian, C. M. (2018) High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described. MycoKeys 39: 97 - 149. https: // doi. org / 10.3897 / mycokeys. 39.26914" type="journal article" year="2018">
Yang
<emphasis id="B909EA8B330EFFCCFDC4F8FD23D2F8F9" box="[591,640,1814,1840]" italics="true" pageId="9" pageNumber="46">et al</emphasis>
. 2018
</bibRefCitation>
,
<bibRefCitation id="EFEC4B68330EFFCCFD5FF8FC22D7F8F9" author="Guo, Y. S. &amp; Crous, P. W. &amp; Bai, Q. &amp; Fu, M. &amp; Yang, M. M. &amp; Wang, X. H. &amp; Du, Y. M. &amp; Hong, N. &amp; Xu, W. X. &amp; Wang, G. P." box="[724,901,1814,1841]" pageId="9" pageNumber="46" pagination="132 - 162" refId="ref7890" refString="Guo, Y. S., Crous, P. W., Bai, Q., Fu, M., Yang, M. M., Wang, X. H., Du, Y. M., Hong, N., Xu, W. X. &amp; Wang, G. P. (2020) High diversity of Diaporthe species associated with pear shoot canker in China. Persoonia-Molecular Phylogeny and Evolution of Fungi 45: 132 - 162. https: // doi. org / 10.3767 / persoonia. 2020.45.05" type="journal article" year="2020">
Guo
<emphasis id="B909EA8B330EFFCCFC80F8FD226EF8F9" box="[779,828,1814,1840]" italics="true" pageId="9" pageNumber="46">et al</emphasis>
. 2020
</bibRefCitation>
,
<bibRefCitation id="EFEC4B68330EFFCCFC1BF8FD2503F8F9" author="Wang, X. &amp; Guo, Y. &amp; Du, Y. &amp; Yang, Z. &amp; Huang, X. &amp; Hong, N. &amp; Xu, W. &amp; Wang, G." box="[912,1105,1814,1841]" pageId="9" pageNumber="46" pagination="77 - 90" refId="ref9902" refString="Wang, X., Guo, Y., Du, Y., Yang, Z., Huang, X., Hong, N., Xu, W. &amp; Wang, G. (2021) Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China. MycoKeys 80: 77 - 90. https: // doi. org / 10.3897 / mycokeys. 80.63816" type="journal article" year="2021">
Wang
<emphasis id="B909EA8B330EFFCCFC5CF8FD255BF8F9" box="[983,1033,1814,1840]" italics="true" pageId="9" pageNumber="46">et al</emphasis>
. 2021
</bibRefCitation>
, this study)
</paragraph>
<paragraph id="8BC23699330EFFCCFF36F8D12057F854" blockId="9.[136,1452,1418,2056]" pageId="9" pageNumber="46">
<emphasis id="B909EA8B330EFFCCFF36F8D1200BF89D" box="[189,345,1850,1876]" italics="true" pageId="9" pageNumber="46">Known hosts:</emphasis>
On symptomatic twigs of
<taxonomicName id="4C7D4D1A330EFFCCFD07F8D12555F89C" authorityName="K.Koch" authorityYear="1869" baseAuthorityName="Wangenh." box="[652,1031,1850,1877]" class="Magnoliopsida" family="Juglandaceae" genus="Carya" kingdom="Plantae" order="Fagales" pageId="9" pageNumber="46" phylum="Tracheophyta" rank="species" species="illinoensis">
<emphasis id="B909EA8B330EFFCCFD07F8D1221CF89D" box="[652,846,1850,1877]" italics="true" pageId="9" pageNumber="46">Carya illinoensis</emphasis>
(
<emphasis id="B909EA8B330EFFCCFCEAF8D022AEF89D" box="[865,1020,1850,1876]" italics="true" pageId="9" pageNumber="46">Juglandaceae</emphasis>
)
</taxonomicName>
, pear shoot canker,
<taxonomicName id="4C7D4D1A330EFFCCFB65F8D124F7F89D" box="[1262,1445,1850,1876]" class="Magnoliopsida" family="Rosaceae" genus="Rosa" kingdom="Plantae" order="Rosales" pageId="9" pageNumber="46" phylum="Tracheophyta" rank="species" species="roxburghii">
<emphasis id="B909EA8B330EFFCCFB65F8D124F7F89D" box="[1262,1445,1850,1876]" italics="true" pageId="9" pageNumber="46">Rosa roxburghii</emphasis>
</taxonomicName>
,
<taxonomicName id="4C7D4D1A330EFFCCFF03F8B52061F8B1" box="[136,307,1887,1912]" class="Magnoliopsida" family="Rosaceae" genus="Prunus" kingdom="Plantae" order="Rosales" pageId="9" pageNumber="46" phylum="Tracheophyta" rank="species" species="persica">
<emphasis id="B909EA8B330EFFCCFF03F8B52061F8B1" box="[136,307,1887,1912]" italics="true" pageId="9" pageNumber="46">Prunus persica</emphasis>
</taxonomicName>
and health stems of
<taxonomicName id="4C7D4D1A330EFFCCFD95F8B52219F8B0" box="[542,843,1886,1913]" class="Magnoliopsida" family="Rutaceae" genus="Orixa" kingdom="Plantae" order="Sapindales" pageId="9" pageNumber="46" phylum="Tracheophyta" rank="species" species="japonica">
<emphasis id="B909EA8B330EFFCCFD95F8B5239BF8B1" box="[542,713,1887,1913]" italics="true" pageId="9" pageNumber="46">Orixa japonica</emphasis>
(
<emphasis id="B909EA8B330EFFCCFD51F8B52210F8B1" box="[730,834,1887,1912]" italics="true" pageId="9" pageNumber="46">Rutaceae</emphasis>
)
</taxonomicName>
(
<bibRefCitation id="EFEC4B68330EFFCCFCD1F8B4254BF8B1" author="Yang, Q. &amp; Fan, X. L. &amp; Guarnaccia, V. &amp; Tian, C. M." box="[858,1049,1886,1913]" pageId="9" pageNumber="46" pagination="97 - 149" refId="ref10010" refString="Yang, Q., Fan, X. L., Guarnaccia, V. &amp; Tian, C. M. (2018) High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described. MycoKeys 39: 97 - 149. https: // doi. org / 10.3897 / mycokeys. 39.26914" type="journal article" year="2018">
Yang
<emphasis id="B909EA8B330EFFCCFC15F8B52282F8B1" box="[926,976,1886,1912]" italics="true" pageId="9" pageNumber="46">et al</emphasis>
. 2018
</bibRefCitation>
,
<bibRefCitation id="EFEC4B68330EFFCCFBAEF8B4258AF8B1" author="Guo, Y. S. &amp; Crous, P. W. &amp; Bai, Q. &amp; Fu, M. &amp; Yang, M. M. &amp; Wang, X. H. &amp; Du, Y. M. &amp; Hong, N. &amp; Xu, W. X. &amp; Wang, G. P." box="[1061,1240,1886,1913]" pageId="9" pageNumber="46" pagination="132 - 162" refId="ref7890" refString="Guo, Y. S., Crous, P. W., Bai, Q., Fu, M., Yang, M. M., Wang, X. H., Du, Y. M., Hong, N., Xu, W. X. &amp; Wang, G. P. (2020) High diversity of Diaporthe species associated with pear shoot canker in China. Persoonia-Molecular Phylogeny and Evolution of Fungi 45: 132 - 162. https: // doi. org / 10.3767 / persoonia. 2020.45.05" type="journal article" year="2020">
Guo
<emphasis id="B909EA8B330EFFCCFBD6F8B525DDF8B1" box="[1117,1167,1886,1912]" italics="true" pageId="9" pageNumber="46">et al</emphasis>
. 2020
</bibRefCitation>
,
<bibRefCitation id="EFEC4B68330EFFCCFB6FF8B524F5F8B1" author="Wang, X. &amp; Guo, Y. &amp; Du, Y. &amp; Yang, Z. &amp; Huang, X. &amp; Hong, N. &amp; Xu, W. &amp; Wang, G." box="[1252,1447,1886,1913]" pageId="9" pageNumber="46" pagination="77 - 90" refId="ref9902" refString="Wang, X., Guo, Y., Du, Y., Yang, Z., Huang, X., Hong, N., Xu, W. &amp; Wang, G. (2021) Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China. MycoKeys 80: 77 - 90. https: // doi. org / 10.3897 / mycokeys. 80.63816" type="journal article" year="2021">
Wang
<emphasis id="B909EA8B330EFFCCFAA7F8B5240CF8B1" box="[1324,1374,1886,1912]" italics="true" pageId="9" pageNumber="46">et al</emphasis>
. 2021
</bibRefCitation>
, this study).
</paragraph>
<paragraph id="8BC23699330EFFCCFF36F84C23DAF7C1" blockId="9.[136,1452,1418,2056]" pageId="9" pageNumber="46">
<emphasis id="B909EA8B330EFFCCFF36F84C20C3F809" box="[189,401,1958,1984]" italics="true" pageId="9" pageNumber="46">Material examined</emphasis>
:
<materialsCitation id="3B153CC4330EFFCCFE14F84C23D6F7C1" ID-GBIF-Occurrence="3757889302" collectingDate="2020-09-14" collectionCode="GZCC" collectorName="Wang, JJ" country="China" location="Qingzhen" pageId="9" pageNumber="46" specimenCode="GZCC 21-1086, GZCC21-1087" specimenCount="1" stateProvince="Guizhou">
<collectingCountry id="F36A7609330EFFCCFE14F84C20A9F809" box="[415,507,1958,1984]" name="China" pageId="9" pageNumber="46">CHINA</collectingCountry>
,
<collectingRegion id="49B9F87B330EFFCCFD8EF84C2380F809" box="[517,722,1958,1984]" country="China" name="Guizhou" pageId="9" pageNumber="46">Guizhou Province</collectingRegion>
,
<location id="8EA26042330EFFCCFD56F84C2218F809" LSID="urn:lsid:plazi:treatment:03D4878F330EFFCEFF03FAF423A0FD79:8EA26042330EFFCCFD56F84C2218F809" box="[733,842,1958,1984]" country="China" name="Qingzhen" pageId="9" pageNumber="46" stateProvince="Guizhou">Qingzhen</location>
city, from the healthy stems of
<taxonomicName id="4C7D4D1A330EFFCCFB23F84D2400F809" box="[1192,1362,1959,1985]" class="Magnoliopsida" family="Rutaceae" genus="Orixa" kingdom="Plantae" order="Sapindales" pageId="9" pageNumber="46" phylum="Tracheophyta" rank="species" species="japonica">
<emphasis id="B909EA8B330EFFCCFB23F84D2400F809" box="[1192,1362,1959,1985]" italics="true" pageId="9" pageNumber="46">Orixa japonica</emphasis>
</taxonomicName>
,
<date id="FFC31059330EFFCCFAD4F84C2191F82D" pageId="9" pageNumber="46" value="2020-09-14">
<collectingDate id="EF87E9B1330EFFCCFAD4F84C2191F82D" pageId="9" pageNumber="46" value="2020-09-14">14 Sep 2020</collectingDate>
</date>
, Hong-Bo
<collectorName id="2688534F330EFFCCFEB7F82120F3F82D" box="[316,417,1994,2021]" pageId="9" pageNumber="46">Wang, JJ</collectorName>
9 (GZAAS 210393, dried culture; living culture:
<specimenCode id="DBDB9EE2330EFFCCFC59F82025D8F82C" box="[978,1162,1994,2021]" collectionCode="GZCC" pageId="9" pageNumber="46">GZCC 211086</specimenCode>
), JJ17 (GZAAS 210394, dried culture; living culture:
<specimenCode id="DBDB9EE2330EFFCCFE41F804232EF7C1" box="[458,636,2030,2056]" collectionCode="GZCC" pageId="9" pageNumber="46">GZCC211087</specimenCode>
)
</materialsCitation>
.
</paragraph>
<caption id="DF026611330DFFCFFF03F89E2444F867" ID-DOI="http://doi.org/10.5281/zenodo.6501350" ID-Zenodo-Dep="6501350" httpUri="https://zenodo.org/record/6501350/files/figure.png" pageId="10" pageNumber="47" startId="10.[136,229,1908,1930]" targetBox="[151,1436,187,1880]" targetPageId="10">
<paragraph id="8BC23699330DFFCFFF03F89E2444F867" blockId="10.[136,1452,1908,1966]" pageId="10" pageNumber="47">
<emphasis id="B909EA8B330DFFCFFF03F89E21AFF840" bold="true" box="[136,253,1908,1930]" pageId="10" pageNumber="47">FIGURE 3.</emphasis>
<taxonomicName id="4C7D4D1A330DFFCFFE89F89E20F9F843" box="[258,427,1908,1930]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="10" pageNumber="47" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330DFFCFFE89F89E20F9F843" box="[258,427,1908,1930]" italics="true" pageId="10" pageNumber="47">Diaporthe caryae</emphasis>
</taxonomicName>
(GZAAS 210393, new host record)
<emphasis id="B909EA8B330DFFCFFC99F89E2265F840" bold="true" box="[786,823,1908,1929]" pageId="10" pageNumber="47">a, b</emphasis>
Colonies on MEA.
<emphasis id="B909EA8B330DFFCFFC7EF89E2552F840" bold="true" box="[1013,1024,1908,1929]" pageId="10" pageNumber="47">c</emphasis>
Immersed conidiomata.
<emphasis id="B909EA8B330DFFCFFB67F89E25ABF840" bold="true" box="[1260,1273,1908,1929]" pageId="10" pageNumber="47">d</emphasis>
Peridium.
<emphasis id="B909EA8B330DFFCFFAE9F89E243FF840" bold="true" box="[1378,1389,1908,1929]" pageId="10" pageNumber="47">e</emphasis>
Alpha and beta conidia
<emphasis id="B909EA8B330DFFCFFEA0F8722014F867" bold="true" box="[299,326,1944,1966]" pageId="10" pageNumber="47">fi</emphasis>
Conidiogenous cells.
<emphasis id="B909EA8B330DFFCFFD90F872236EF864" bold="true" box="[539,572,1944,1966]" pageId="10" pageNumber="47">j, k</emphasis>
Alpha conidia.
<emphasis id="B909EA8B330DFFCFFD5EF87223A6F864" bold="true" box="[725,756,1944,1965]" pageId="10" pageNumber="47">ln</emphasis>
Beta conidia. Scale bars:
<emphasis id="B909EA8B330DFFCFFC65F872256CF864" bold="true" box="[1006,1086,1944,1965]" pageId="10" pageNumber="47">d, e, ln</emphasis>
= 10 µm,
<emphasis id="B909EA8B330DFFCFFB29F8722596F867" bold="true" box="[1186,1220,1944,1966]" pageId="10" pageNumber="47">fk</emphasis>
= 5 µm.
</paragraph>
</caption>
<paragraph id="8BC23699330DFFCEFF36F83722DDFE59" blockId="10.[136,1452,2012,2075]" lastBlockId="11.[136,1452,158,689]" lastPageId="11" lastPageNumber="48" pageId="10" pageNumber="47">
Notes:
<taxonomicName id="4C7D4D1A330DFFCFFE84F83720A8F83E" box="[271,506,2013,2039]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="10" pageNumber="47" phylum="Ascomycota" rank="species" species="sackstonii">
<emphasis id="B909EA8B330DFFCFFE84F83720A8F83E" box="[271,506,2013,2039]" italics="true" pageId="10" pageNumber="47">Diaporthe sackstonii</emphasis>
</taxonomicName>
was introduced by
<bibRefCitation id="EFEC4B68330DFFCFFD52F83722B7F83E" author="Thompson, S. M. &amp; Tan, Y. P. &amp; Shivas, R. G. &amp; Neate, S. M. &amp; Morin, L. &amp; Bissett, A. &amp; Aitken, E. A. B." box="[729,997,2012,2039]" pageId="10" pageNumber="47" pagination="39 - 49" refId="ref9396" refString="Thompson, S. M., Tan, Y. P., Shivas, R. G., Neate, S. M., Morin, L., Bissett, A. &amp; Aitken, E. A. B. (2015) Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia. Persoonia: Molecular Phylogeny and Evolution of Fungi 35: 39 - 49. https: // doi. org / 10.3767 / 003158515 X 687506" type="journal article" year="2015">
Thompson
<emphasis id="B909EA8B330DFFCFFCD2F83722D9F83E" box="[857,907,2013,2039]" italics="true" pageId="10" pageNumber="47">et al</emphasis>
. (2015)
</bibRefCitation>
with only producing alpha conidia. The strain (GZCC 190129) which was reported by
<bibRefCitation id="EFEC4B68330DFFCFFD10F7EB2291F7D2" author="Dissanayake, A. J. &amp; Chen, Y. Y. &amp; Liu, J. K." box="[667,963,2049,2075]" pageId="10" pageNumber="47" pagination="251" refId="ref7469" refString="Dissanayake, A. J., Chen, Y. Y. &amp; Liu, J. K. (2020 a) Unravelling Diaporthe species associated with woody hosts from Karst Formations (Guizhou) in China. Journal of Fungi 6 (4): 251. https: // doi. org / 10.3390 / jof 6040251" type="journal article" year="2020">
Dissanayake
<emphasis id="B909EA8B330DFFCFFCA5F7EB220DF7D2" box="[814,863,2049,2075]" italics="true" pageId="10" pageNumber="47">et al</emphasis>
. (2020a)
</bibRefCitation>
without any morphological description was clustered together with taxa of
<taxonomicName id="4C7D4D1A330CFFCEFE64FF75230DFF71" box="[495,607,159,184]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFE64FF75230DFF71" box="[495,607,159,184]" italics="true" pageId="11" pageNumber="48">D. caryae</emphasis>
</taxonomicName>
in our study (
<figureCitation id="13462A1C330CFFCEFC88FF7422D2FF71" box="[771,896,158,184]" captionStart="FIGURE 1" captionStartId="6.[136,229,1942,1964]" captionTargetBox="[299,1353,213,1915]" captionTargetId="figure-24@6.[151,1436,187,1915]" captionTargetPageId="6" captionText="FIGURE 1. Maximum likelihood tree generated by RAxML based on the combined ITS, tef1-α, β-tub, cal, and his3. Bootstrap support values for ML and MP equal to or greater than 70% and Bayesian posterior probabilities equal to or greater than 0.95 are given near nodes as ML/PP/MP, respectively. The tree was rooted with Diaporthella corylina (CBS 121124) and Diaporthella cryptica (CBS 140348). The newly generated strains are shown in red and the ex-type strains are in bold." figureDoi="http://doi.org/10.5281/zenodo.6501344" httpUri="https://zenodo.org/record/6501344/files/figure.png" pageId="11" pageNumber="48">FIGURE 1</figureCitation>
). A comparison of nucleotide base pairs of ITS,
<emphasis id="B909EA8B330CFFCEFF03FF282198FF15" box="[136,202,194,220]" italics="true" pageId="11" pageNumber="48">tef1-α</emphasis>
, and
<emphasis id="B909EA8B330CFFCEFE82FF282016FF15" box="[265,324,194,220]" italics="true" pageId="11" pageNumber="48">β-tub</emphasis>
between the strain (GZCC 190129) and the
<typeStatus id="54C6883B330CFFCEFCCCFF292225FF14" box="[839,887,195,221]" pageId="11" pageNumber="48">type</typeStatus>
species of
<taxonomicName id="4C7D4D1A330CFFCEFC7DFF2925DBFF15" box="[1014,1161,194,221]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="sackstonii">
<emphasis id="B909EA8B330CFFCEFC7DFF292558FF15" box="[1014,1034,195,220]" italics="true" pageId="11" pageNumber="48">D</emphasis>
.
<emphasis id="B909EA8B330CFFCEFB92FF2825DBFF15" box="[1049,1161,194,220]" italics="true" pageId="11" pageNumber="48">sackstonii</emphasis>
</taxonomicName>
and
<taxonomicName id="4C7D4D1A330CFFCEFB4AFF29247DFF15" box="[1217,1327,195,221]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFB4AFF292587FF15" box="[1217,1237,195,220]" italics="true" pageId="11" pageNumber="48">D</emphasis>
.
<emphasis id="B909EA8B330CFFCEFB68FF29247DFF15" box="[1251,1327,195,220]" italics="true" pageId="11" pageNumber="48">caryae</emphasis>
</taxonomicName>
were done based on BLASTn algorithms for aligning two sequences in NCBI. In ITS,
<taxonomicName id="4C7D4D1A330CFFCEFC78FF0D25D5FEC9" box="[1011,1159,230,256]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="sackstonii">
<emphasis id="B909EA8B330CFFCEFC78FF0D25D5FEC9" box="[1011,1159,230,256]" italics="true" pageId="11" pageNumber="48">D. sackstonii</emphasis>
</taxonomicName>
(GZCC 190129) differs from the
<typeStatus id="54C6883B330CFFCEFF79FEE12070FEEC" box="[242,290,267,293]" pageId="11" pageNumber="48">type</typeStatus>
species of
<taxonomicName id="4C7D4D1A330CFFCEFE2FFEE1236AFEED" box="[420,568,266,292]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="sackstonii">
<emphasis id="B909EA8B330CFFCEFE2FFEE1236AFEED" box="[420,568,266,292]" italics="true" pageId="11" pageNumber="48">D. sackstonii</emphasis>
</taxonomicName>
(BRIP 54669b) and
<taxonomicName id="4C7D4D1A330CFFCEFCACFEE122C5FEED" box="[807,919,267,292]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFCACFEE122C5FEED" box="[807,919,267,292]" italics="true" pageId="11" pageNumber="48">D. caryae</emphasis>
</taxonomicName>
(CFCC 52563) in 1/469 bp (0.21%), 3/452 bp (0.66%), respectively. In
<emphasis id="B909EA8B330CFFCEFE2AFEC420B1FE81" box="[417,483,302,328]" italics="true" pageId="11" pageNumber="48">tef1-α</emphasis>
, they are different in 13/311 bp (4.18%), 1/311 bp (0.32%), respectively. In
<emphasis id="B909EA8B330CFFCEFAB8FEC4243CFE81" box="[1331,1390,302,328]" italics="true" pageId="11" pageNumber="48">β-tub</emphasis>
, they are different in 25/701 bp (0.036%) and 10/400 bp (2.5%), respectively. We therefore suggested that the identification of the strain (
<taxonomicName id="4C7D4D1A330CFFCEFE95FE9D2350FE59" authority="GZCC" authorityName="GZCC" box="[286,514,374,400]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="sackstonii">
<emphasis id="B909EA8B330CFFCEFE95FE9D20E2FE59" box="[286,432,374,400]" italics="true" pageId="11" pageNumber="48">D. sackstonii</emphasis>
GZCC
</taxonomicName>
190129) may need to re-evaluate.
</paragraph>
<paragraph id="8BC23699330CFFCEFF36FE7023A0FD79" blockId="11.[136,1452,158,689]" pageId="11" pageNumber="48">
<taxonomicName id="4C7D4D1A330CFFCEFF36FE7020DAFE7D" box="[189,392,410,436]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFF36FE7020DAFE7D" box="[189,392,410,436]" italics="true" pageId="11" pageNumber="48">Diaporthe caryae</emphasis>
</taxonomicName>
was firstly described from symptomatic twigs of
<taxonomicName id="4C7D4D1A330CFFCEFC5CFE7125C9FE7D" authorityName="K.Koch" authorityYear="1869" baseAuthorityName="Wangenh." box="[983,1179,410,437]" class="Magnoliopsida" family="Juglandaceae" genus="Carya" kingdom="Plantae" order="Fagales" pageId="11" pageNumber="48" phylum="Tracheophyta" rank="species" species="illinoensis">
<emphasis id="B909EA8B330CFFCEFC5CFE7125C9FE7D" box="[983,1179,410,437]" italics="true" pageId="11" pageNumber="48">Carya illinoensis</emphasis>
</taxonomicName>
by
<bibRefCitation id="EFEC4B68330CFFCEFB46FE7124FAFE7D" author="Yang, Q. &amp; Fan, X. L. &amp; Guarnaccia, V. &amp; Tian, C. M." box="[1229,1448,410,437]" pageId="11" pageNumber="48" pagination="97 - 149" refId="ref10010" refString="Yang, Q., Fan, X. L., Guarnaccia, V. &amp; Tian, C. M. (2018) High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described. MycoKeys 39: 97 - 149. https: // doi. org / 10.3897 / mycokeys. 39.26914" type="journal article" year="2018">
Yang
<emphasis id="B909EA8B330CFFCEFA99FE71241DFE7D" box="[1298,1359,410,436]" italics="true" pageId="11" pageNumber="48">et al.</emphasis>
(2018)
</bibRefCitation>
. Morphologically, the two new collections (GZCC 211086, GZCC 211087) are similar to the
<typeStatus id="54C6883B330CFFCEFB3EFE542445FE11" box="[1205,1303,446,472]" pageId="11" pageNumber="48" type="holotype">holotype</typeStatus>
of
<taxonomicName id="4C7D4D1A330CFFCEFAB6FE5524F9FE11" box="[1341,1451,447,472]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFAB6FE5524F9FE11" box="[1341,1451,447,472]" italics="true" pageId="11" pageNumber="48">D. caryae</emphasis>
</taxonomicName>
in conidiophores and conidia. Phylogenetically, GZCC 211086 and GZCC 211087 are grouped with
<taxonomicName id="4C7D4D1A330CFFCEFA93FE0924F9FE35" box="[1304,1451,482,508]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="sackstonii">
<emphasis id="B909EA8B330CFFCEFA93FE0924F9FE35" box="[1304,1451,482,508]" italics="true" pageId="11" pageNumber="48">D. sackstonii</emphasis>
</taxonomicName>
(GZCC 190129) and
<taxonomicName id="4C7D4D1A330CFFCEFE0BFDED20BEFDE9" box="[384,492,519,544]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFE0BFDED20BEFDE9" box="[384,492,519,544]" italics="true" pageId="11" pageNumber="48">D. caryae</emphasis>
</taxonomicName>
(CFCC 52563, CFCC 52564) in the phylogenetic tree with good support. Furthermore, we compared the new collections with the
<typeStatus id="54C6883B330CFFCEFDE1FDC0239EFD8D" box="[618,716,554,580]" pageId="11" pageNumber="48" type="holotype">holotype</typeStatus>
of
<taxonomicName id="4C7D4D1A330CFFCEFD78FDC12230FD8D" box="[755,866,555,580]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFD78FDC12230FD8D" box="[755,866,555,580]" italics="true" pageId="11" pageNumber="48">D. caryae</emphasis>
</taxonomicName>
(CFCC 52563) using single gene data, and they are 100%, 1.8% (5/273), 0.7% (5/699) similar in ITS,
<emphasis id="B909EA8B330CFFCEFD55FDA42272FDA1" box="[734,800,590,616]" italics="true" pageId="11" pageNumber="48">tef1-α</emphasis>
, and
<emphasis id="B909EA8B330CFFCEFCEDFDA422F3FDA1" box="[870,929,590,616]" italics="true" pageId="11" pageNumber="48">β-tub</emphasis>
, respectively. Based on the morphology and molecular data analyses, the two new collections (GZCC 211086, GZCC 211087) are considered as
<taxonomicName id="4C7D4D1A330CFFCEFB74FD99243FFD45" box="[1279,1389,627,652]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFB74FD99243FFD45" box="[1279,1389,627,652]" italics="true" pageId="11" pageNumber="48">D. caryae</emphasis>
</taxonomicName>
. This is the first report of
<taxonomicName id="4C7D4D1A330CFFCEFEECFD7D2087FD79" box="[359,469,663,688]" class="Sordariomycetes" family="Diaporthaceae" genus="Diaporthe" kingdom="Fungi" order="Diaporthales" pageId="11" pageNumber="48" phylum="Ascomycota" rank="species" species="caryae">
<emphasis id="B909EA8B330CFFCEFEECFD7D2087FD79" box="[359,469,663,688]" italics="true" pageId="11" pageNumber="48">D. caryae</emphasis>
</taxonomicName>
on
<taxonomicName id="4C7D4D1A330CFFCEFD8BFD7D23D6FD79" box="[512,644,663,689]" class="Magnoliopsida" family="Rutaceae" genus="Orixa" kingdom="Plantae" order="Sapindales" pageId="11" pageNumber="48" phylum="Tracheophyta" rank="species" species="japonica">
<emphasis id="B909EA8B330CFFCEFD8BFD7D23D6FD79" box="[512,644,663,689]" italics="true" pageId="11" pageNumber="48">O. japonica</emphasis>
</taxonomicName>
in
<collectingCountry id="F36A7609330CFFCEFD23FD7C23BDFD79" box="[680,751,662,688]" name="China" pageId="11" pageNumber="48">China</collectingCountry>
.
</paragraph>
</subSubSection>
</treatment>
</document>