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<document ID-DOI="10.12782/specdiv.27.61" ID-ISSN="2189-7301" ID-Zenodo-Dep="7175435" approvalRequired="1" approvalRequired_for_textStreams="1" checkinTime="1665293609630" checkinUser="felipe" docAuthor="Nitta, Masato" docDate="2022" docId="03BE879DEA47FFF9FF55412099DF144D" docLanguage="en" docName="SpeciesDiversity.27.1.61-69.pdf" docOrigin="Species Diversity 27 (1)" docSource="http://dx.doi.org/10.12782/specdiv.27.61" docStyle="DocumentStyle:27FECD2C3CD153DA405029F2D33C9514.5:SpeciesDiversity.2012-.journal_article" docStyleId="27FECD2C3CD153DA405029F2D33C9514" docStyleName="SpeciesDiversity.2012-.journal_article" docStyleVersion="5" docTitle="Epistylis wuhanensis Wang, Zhou, Guo, and Gu 2017" docType="treatment" docVersion="2" lastPageNumber="63" masterDocId="FF87FFE5EA45FFFFFFE942719B5E152D" masterDocTitle="First Japanese Record of Epistylis wuhanensis (Ciliophora: Epistylididae) Attached to Lernaea cyprinacea (Copepoda), with a List of Epistylis Species Attached to Metazoans in Japan" masterLastPageNumber="69" masterPageNumber="61" pageNumber="63" updateTime="1665311387584" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title>First Japanese Record of Epistylis wuhanensis (Ciliophora: Epistylididae) Attached to Lernaea cyprinacea (Copepoda), with a List of Epistylis Species Attached to Metazoans in Japan</mods:title>
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<mods:name type="personal">
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<mods:roleTerm>Author</mods:roleTerm>
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<mods:namePart>Nitta, Masato</mods:namePart>
<mods:affiliation>Graduate School of Science, Kobe University, 1 - 1 Rokkodai, Nada, Kobe, Hyogo 657 - 8501, Japan &amp; Present address: Setouchi Parasite Biodiversity Laboratory, 3 - 2 - 20 - 103 Ushita-Honmachi, Higashi-ku, Hiroshima 732 - 0066, Japan &amp; Laboratory of Bioresources, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi 444 - 8787, Japan E-mail: licht. bsn. mono @ gmail. com</mods:affiliation>
<mods:nameIdentifier type="email">bsn.mono@gmail.com</mods:nameIdentifier>
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<mods:typeOfResource>text</mods:typeOfResource>
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<mods:title>Species Diversity</mods:title>
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<mods:date>2022</mods:date>
<mods:detail type="pubDate">
<mods:number>2022-03-10</mods:number>
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<mods:detail type="volume">
<mods:number>27</mods:number>
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<mods:url>http://dx.doi.org/10.12782/specdiv.27.61</mods:url>
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<mods:identifier type="DOI">10.12782/specdiv.27.61</mods:identifier>
<mods:identifier type="ISSN">2189-7301</mods:identifier>
<mods:identifier type="Zenodo-Dep">7175435</mods:identifier>
</mods:mods>
<treatment LSID="urn:lsid:plazi:treatment:03BE879DEA47FFF9FF55412099DF144D" httpUri="http://treatment.plazi.org/id/03BE879DEA47FFF9FF55412099DF144D" lastPageId="6" lastPageNumber="63" pageId="2" pageNumber="63">
<subSubSection pageId="2" pageNumber="63" type="nomenclature">
<paragraph blockId="2.[188,735,849,906]" pageId="2" pageNumber="63">
<heading centered="true" fontSize="10" level="2" pageId="2" pageNumber="63" reason="2">
<taxonomicName authority="Wang, Zhou, Guo, and Gu, 2017" authorityName="Wang, Zhou, Guo, and Gu" authorityYear="2017" box="[188,735,849,873]" class="Oligohymenophorea" family="Epistylididae" genus="Epistylis" kingdom="Chromista" order="Peritrichida" pageId="2" pageNumber="63" phylum="Ciliophora" rank="species" species="wuhanensis">
<emphasis bold="true" box="[188,403,849,873]" italics="true" pageId="2" pageNumber="63">Epistylis wuhanensis</emphasis>
<bibRefCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." box="[409,735,849,873]" pageId="2" pageNumber="63" pagination="394 - 406" refId="ref7636" refString="Wang, Z., Zhou, T., Guo, Q., and Gu, Z. 2017. Description of a new freshwater ciliate Epistylis wuhanensis n. sp. (Ciliophora, Peritrichia) from China, with a focus on phylogenetic relationships within family Epistylididae. Journal of Eukaryotic Microbiology 64: 394 - 406." type="journal article" year="2017">Wang, Zhou, Guo, and Gu, 2017</bibRefCitation>
</taxonomicName>
(
<figureCitation box="[282,343,881,906]" captionStart="Fig" captionStartId="4.[156,187,1960,1982]" captionTargetBox="[189,1399,207,1920]" captionTargetId="figure-8@4.[188,1400,205,1921]" captionTargetPageId="4" captionText="Fig. 1. Scanning electron micrographs of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan. A, E. wuhanensis attached to exposed cephalothorax of Lernaea cyprinacea; B, extended zooid, lateral view; C, contracted zooid, lateral view; D, extended zooid, apical view; E, scopula of extended zooid. Abbreviations: AMZ, adoral zone of membranelles; ATB, aboral trochal band; PD, peristomial disc; PL, peristomial lip; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175437" httpUri="https://zenodo.org/record/7175437/files/figure.png" pageId="2" pageNumber="63">Figs 1</figureCitation>
,
<figureCitation box="[355,369,882,906]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">2</figureCitation>
; Supplemental Movies 1–3)
</heading>
</paragraph>
</subSubSection>
<subSubSection pageId="2" pageNumber="63" type="reference_group">
<paragraph blockId="2.[156,767,946,1068]" pageId="2" pageNumber="63">
<treatmentCitationGroup pageId="2" pageNumber="63">
<treatmentCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." page="400" pageId="2" pageNumber="63" year="2017">
<taxonomicName authority="Wang, Zhou, Guo, and Gu, 2017: 396 - 400" authorityName="Wang, Zhou, Guo, and Gu" authorityPageNumber="396 - 400" authorityYear="2017" class="Oligohymenophorea" family="Epistylididae" genus="Epistylis" kingdom="Chromista" order="Peritrichida" pageId="2" pageNumber="63" phylum="Ciliophora" rank="species" species="wuhanensis">
<emphasis box="[156,361,947,971]" italics="true" pageId="2" pageNumber="63">Epistylis wuhanensis</emphasis>
<bibRefCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." pageId="2" pageNumber="63" pagination="394 - 406" refId="ref7636" refString="Wang, Z., Zhou, T., Guo, Q., and Gu, Z. 2017. Description of a new freshwater ciliate Epistylis wuhanensis n. sp. (Ciliophora, Peritrichia) from China, with a focus on phylogenetic relationships within family Epistylididae. Journal of Eukaryotic Microbiology 64: 394 - 406." type="journal article" year="2017">Wang, Zhou, Guo, and Gu, 2017: 396– 400</bibRefCitation>
</taxonomicName>
</treatmentCitation>
, figs 1–5.
</treatmentCitationGroup>
</paragraph>
<paragraph blockId="2.[156,767,946,1068]" pageId="2" pageNumber="63">
Peritrich ciliates:
<bibRefCitation author="Nagasawa, K. &amp; Nitta, M." box="[331,637,1012,1036]" pageId="2" pageNumber="63" pagination="147 - 150" refId="ref6511" refString="Nagasawa, K. and Nitta, M. 2019. First record of the freshwater fish parasite Lernaea cyprinacea (Copepoda: Lernaeidae) in Wakayama Prefecture, central Japan. Nature of Kagoshima 46: 147 - 150. [In Japanese with English abstract]" type="journal article" year="2019">Nagasawa and Nitta 2019: 148</bibRefCitation>
, 149, fig. 1C, D.
</paragraph>
</subSubSection>
<subSubSection pageId="2" pageNumber="63" type="description">
<paragraph blockId="2.[156,773,1109,2077]" lastBlockId="2.[820,1432,198,2077]" pageId="2" pageNumber="63">
<emphasis bold="true" box="[181,313,1109,1133]" pageId="2" pageNumber="63">Description.</emphasis>
Colony up to
<quantity box="[492,548,1109,1133]" metricMagnitude="-3" metricUnit="m" metricValue="1.0" pageId="2" pageNumber="63" unit="mm" value="1.0">1 mm</quantity>
in height and comprised of less than 60 zooids, branched dichotomously with secondary or more stalks (
<figureCitation box="[432,513,1174,1198]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2A</figureCitation>
); span between first and secondary stalks relatively long, occupying half to twothirds of the total length (
<figureCitation box="[436,519,1239,1263]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2A</figureCitation>
). Stalks non-contractile (Supplemental Movie 1), transversely wrinkled weakly when the stalk curved (
<figureCitation box="[394,473,1304,1328]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2A</figureCitation>
), with longitudinal striations on the surface (
<figureCitation box="[323,403,1337,1361]" captionStart="Fig" captionStartId="4.[156,187,1960,1982]" captionTargetBox="[189,1399,207,1920]" captionTargetId="figure-8@4.[188,1400,205,1921]" captionTargetPageId="4" captionText="Fig. 1. Scanning electron micrographs of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan. A, E. wuhanensis attached to exposed cephalothorax of Lernaea cyprinacea; B, extended zooid, lateral view; C, contracted zooid, lateral view; D, extended zooid, apical view; E, scopula of extended zooid. Abbreviations: AMZ, adoral zone of membranelles; ATB, aboral trochal band; PD, peristomial disc; PL, peristomial lip; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175437" httpUri="https://zenodo.org/record/7175437/files/figure.png" pageId="2" pageNumber="63">Fig. 1A</figureCitation>
), 11.3–25.9 (15.7, n=11) width in branched regions. Expended zooid elongate bell-shaped to elliptical (
<figureCitation box="[256,334,1402,1426]" captionStart="Fig" captionStartId="4.[156,187,1960,1982]" captionTargetBox="[189,1399,207,1920]" captionTargetId="figure-8@4.[188,1400,205,1921]" captionTargetPageId="4" captionText="Fig. 1. Scanning electron micrographs of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan. A, E. wuhanensis attached to exposed cephalothorax of Lernaea cyprinacea; B, extended zooid, lateral view; C, contracted zooid, lateral view; D, extended zooid, apical view; E, scopula of extended zooid. Abbreviations: AMZ, adoral zone of membranelles; ATB, aboral trochal band; PD, peristomial disc; PL, peristomial lip; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175437" httpUri="https://zenodo.org/record/7175437/files/figure.png" pageId="2" pageNumber="63">Figs 1B</figureCitation>
,
<figureCitation box="[345,406,1402,1426]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">2A–D</figureCitation>
, Supplemental Movies 2, 3), 163.1– 220.3 (190.1, n=15) length in vivo, 128.0–181.9 (150.2, n= 6) length in fixed specimens; in vivo, half of specimens widest close to the single-layered, thin peristomial lip 41.6–56.1 (47, n=15) in width, 36.1–56.9 (46.3, n=15) in width at the mid-point; peristomial lip in fixed specimens 36.2–54.7 (44.7, n=6) wide, widest at the mid-point (47.1–64.0, 55.7, n=6); average length to width ratio in vivo 3.3–5.3 (4.1): 1 (n=15) at mid-point, 2.1–3.5 (2.7):1 (n=6) in fixed specimens. Contracted zooid ovoid (
<figureCitation box="[494,576,1695,1719]" captionStart="Fig" captionStartId="4.[156,187,1960,1982]" captionTargetBox="[189,1399,207,1920]" captionTargetId="figure-8@4.[188,1400,205,1921]" captionTargetPageId="4" captionText="Fig. 1. Scanning electron micrographs of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan. A, E. wuhanensis attached to exposed cephalothorax of Lernaea cyprinacea; B, extended zooid, lateral view; C, contracted zooid, lateral view; D, extended zooid, apical view; E, scopula of extended zooid. Abbreviations: AMZ, adoral zone of membranelles; ATB, aboral trochal band; PD, peristomial disc; PL, peristomial lip; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175437" httpUri="https://zenodo.org/record/7175437/files/figure.png" pageId="2" pageNumber="63">Fig. 1C</figureCitation>
). Peristomial disk mammilla-shaped, 11.2–13.4×14.7–18.4 (12.1×16.1, n=5), surrounded by adoral zone of membranelles (
<figureCitation box="[637,721,1760,1784]" captionStart="Fig" captionStartId="4.[156,187,1960,1982]" captionTargetBox="[189,1399,207,1920]" captionTargetId="figure-8@4.[188,1400,205,1921]" captionTargetPageId="4" captionText="Fig. 1. Scanning electron micrographs of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan. A, E. wuhanensis attached to exposed cephalothorax of Lernaea cyprinacea; B, extended zooid, lateral view; C, contracted zooid, lateral view; D, extended zooid, apical view; E, scopula of extended zooid. Abbreviations: AMZ, adoral zone of membranelles; ATB, aboral trochal band; PD, peristomial disc; PL, peristomial lip; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175437" httpUri="https://zenodo.org/record/7175437/files/figure.png" pageId="2" pageNumber="63">Figs 1A</figureCitation>
,
<figureCitation captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">2B, D</figureCitation>
). Macronucleus lying in the middle or upper thirds of cell, commonly C-shaped or sometimes J-shaped (
<figureCitation box="[639,755,1825,1849]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2C, D</figureCitation>
). Cytopharynx lying in upper third of expended zooid (
<figureCitation captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2B</figureCitation>
). Contractile vacuole between macronucleus and peristomial lip (
<figureCitation box="[251,329,1922,1947]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2B</figureCitation>
). Aboral trochal band lying in lower third to fourth of zooid (
<figureCitation box="[355,464,1955,1979]" captionStart="Fig" captionStartId="4.[156,187,1960,1982]" captionTargetBox="[189,1399,207,1920]" captionTargetId="figure-8@4.[188,1400,205,1921]" captionTargetPageId="4" captionText="Fig. 1. Scanning electron micrographs of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan. A, E. wuhanensis attached to exposed cephalothorax of Lernaea cyprinacea; B, extended zooid, lateral view; C, contracted zooid, lateral view; D, extended zooid, apical view; E, scopula of extended zooid. Abbreviations: AMZ, adoral zone of membranelles; ATB, aboral trochal band; PD, peristomial disc; PL, peristomial lip; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175437" httpUri="https://zenodo.org/record/7175437/files/figure.png" pageId="2" pageNumber="63">Figs 1B, C</figureCitation>
,
<figureCitation box="[476,539,1955,1979]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">2B–D</figureCitation>
). Pellicle with circular transverse (
<figureCitation box="[276,417,1987,2012]" captionStart="Fig" captionStartId="4.[156,187,1960,1982]" captionTargetBox="[189,1399,207,1920]" captionTargetId="figure-8@4.[188,1400,205,1921]" captionTargetPageId="4" captionText="Fig. 1. Scanning electron micrographs of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan. A, E. wuhanensis attached to exposed cephalothorax of Lernaea cyprinacea; B, extended zooid, lateral view; C, contracted zooid, lateral view; D, extended zooid, apical view; E, scopula of extended zooid. Abbreviations: AMZ, adoral zone of membranelles; ATB, aboral trochal band; PD, peristomial disc; PL, peristomial lip; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175437" httpUri="https://zenodo.org/record/7175437/files/figure.png" pageId="2" pageNumber="63">Fig. 1B, C, E</figureCitation>
); transverse striations distributed equally, 138–164 (149.2, n=5) striations between peristome and aboral trochal band, 92–102 (95.6, n=5) between aboral trochal band and scopula. Longitudinal fibers continually extending across the body, from peristome to scopula (
<figureCitation captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2C, D</figureCitation>
).
</paragraph>
<paragraph blockId="2.[820,1432,198,2077]" pageId="2" pageNumber="63">
Infundibular polykineties lying in upper third of expended zooid (
<figureCitation box="[976,1057,328,352]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2D</figureCitation>
). Haplokinety and polykinety parallel, both making ca. 1.6–1.8 turns around peristome, then entering infundibulum (
<figureCitation box="[1075,1154,393,417]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2F</figureCitation>
). Germinal kinety parallel with haplokinety in infundibular region, curving anteriorly to form half-circle anterior to infundibular polykinety 2 (P2), then crossing to infundibular polykinety 1 (P1) and P2 (
<figureCitation box="[862,938,523,548]" captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2E</figureCitation>
). Each infundibular polykinety consists of three rows of kinetosomes in lower third of infundibulum (
<figureCitation captionStart="Fig" captionStartId="5.[156,187,1882,1904]" captionTargetBox="[189,1399,206,1842]" captionTargetId="figure-3@5.[188,1400,205,1843]" captionTargetPageId="5" captionText="Fig. 2. In vivo and protargol stained specimens of Epistylis wuhanensis Wang, Zhou, Guo, and Gu, 2017 from Japan (MPM 21760). A, Colony of zooids; B–D, extended zooid, lateral view; E, abstomal region of infraciliature polykineties; F, extended zooid, apical view. Abbreviations: ATB, aboral trochal band; AZM, adoral zone of membranelles; CV: contractile vacuole; CY, cytopharynx; G, germinal kinety; H, haplokinety; MN, macronucleus; P1–3, infundibular polykineties 1–3; PD, peristomial disc; PL, peristomial lip; Po, polykinety; SC, scopula; ST, stalk; ZO, zooid." figureDoi="http://doi.org/10.5281/zenodo.7175439" httpUri="https://zenodo.org/record/7175439/files/figure.png" pageId="2" pageNumber="63">Fig. 2E</figureCitation>
); P1 longest, consisting of three rows, extending laterally, then bending downward, extending shortly, and terminating at end of infundibulum. P2 almost parallel to lateral rows of P1, terminating at posterior curvature of P1. Polykinety 3 (P3) shortest, about half the length of P2, extending to the end of P2, then bending downward with P1 and terminating near the end of P1.
</paragraph>
<paragraph blockId="2.[820,1432,198,2077]" pageId="2" pageNumber="63">
<emphasis bold="true" box="[846,1113,816,840]" pageId="2" pageNumber="63">Deposition of specimens.</emphasis>
<accessionNumber box="[1139,1265,816,840]" httpUri="http://www.ncbi.nlm.nih.gov/protein/MPM21760" pageId="2" pageNumber="63">MPM 21760</accessionNumber>
(mounted specimens) and
<accessionNumber box="[942,1069,849,873]" httpUri="http://www.ncbi.nlm.nih.gov/protein/MPM21761" pageId="2" pageNumber="63">MPM 21761</accessionNumber>
a [preserved in 70% ethanol with
<taxonomicName authorityName="Linnaeus" authorityYear="1758" class="Hexanauplia" family="Lernaeidae" genus="Lernaea" kingdom="Animalia" order="Cyclopoida" pageId="2" pageNumber="63" phylum="Arthropoda" rank="species" species="cyprinacea">
<emphasis italics="true" pageId="2" pageNumber="63">L. cyprinacea</emphasis>
</taxonomicName>
(
<accessionNumber box="[940,1074,881,905]" httpUri="http://www.ncbi.nlm.nih.gov/protein/MPM21761" pageId="2" pageNumber="63">MPM 21761</accessionNumber>
b)].
</paragraph>
<paragraph blockId="2.[820,1432,198,2077]" pageId="2" pageNumber="63">
<emphasis bold="true" box="[846,1003,913,938]" pageId="2" pageNumber="63">Sequence data.</emphasis>
<materialsCitation accessionNumber="LC570274, LC570273" latitude="-5.8" pageId="2" pageNumber="63" specimenCount="1">
<accessionNumber box="[1029,1135,914,938]" httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/LC570274" pageId="2" pageNumber="63">LC570274</accessionNumber>
: partial 18S rDNA (1653 bp);
<accessionNumber box="[820,926,946,970]" httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/LC570273" pageId="2" pageNumber="63">LC570273</accessionNumber>
: partial 18S (14 bp), complete ITS1 (140 bp),
<geoCoordinate box="[820,863,979,1003]" degrees="5.8" direction="south" orientation="latitude" pageId="2" pageNumber="63" precision="5555" value="-5.8">5.8S</geoCoordinate>
rDNA (109 bp), ITS2, (170 bp), and partial 28S rDNA (34 bp)
</materialsCitation>
.
</paragraph>
<paragraph blockId="2.[820,1432,198,2077]" pageId="2" pageNumber="63">
<emphasis bold="true" box="[846,1168,1044,1068]" pageId="2" pageNumber="63">Molecular data comparison.</emphasis>
As found through a BLAST search, the newly obtained partial 18S rDNA sequence (
<accessionNumber box="[910,1018,1109,1133]" httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/LC570274" pageId="2" pageNumber="63">LC570274</accessionNumber>
) was identical, by original description, to
<taxonomicName authorityName="Wang, Zhou, Guo, and Gu" authorityYear="2017" box="[853,1001,1141,1165]" class="Oligohymenophorea" family="Epistylididae" genus="Epistylis" kingdom="Chromista" order="Peritrichida" pageId="2" pageNumber="63" phylum="Ciliophora" rank="species" species="wuhanensis">
<emphasis box="[853,1001,1141,1165]" italics="true" pageId="2" pageNumber="63">E. wuhanensis</emphasis>
</taxonomicName>
with 100% coverage (
<accessionNumber box="[1245,1358,1141,1165]" httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/KU869709" pageId="2" pageNumber="63">KU869709</accessionNumber>
:
<bibRefCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." pageId="2" pageNumber="63" pagination="394 - 406" refId="ref7636" refString="Wang, Z., Zhou, T., Guo, Q., and Gu, Z. 2017. Description of a new freshwater ciliate Epistylis wuhanensis n. sp. (Ciliophora, Peritrichia) from China, with a focus on phylogenetic relationships within family Epistylididae. Journal of Eukaryotic Microbiology 64: 394 - 406." type="journal article" year="2017">Wang et al. 2017</bibRefCitation>
) and
<taxonomicName authorityName="Wang, Zhou, Guo, and Gu" authorityYear="2017" box="[1001,1182,1174,1198]" class="Oligohymenophorea" family="Epistylididae" genus="Epistylis" isUncertain="true" kingdom="Chromista" order="Peritrichida" pageId="2" pageNumber="63" phylum="Ciliophora" rank="species" species="wuhanensis">
<emphasis box="[1001,1015,1175,1198]" italics="true" pageId="2" pageNumber="63">E</emphasis>
. cf.
<emphasis box="[1066,1182,1174,1198]" italics="true" pageId="2" pageNumber="63">wuhanensis</emphasis>
</taxonomicName>
collected in the
<collectingCountry box="[1368,1432,1174,1198]" name="United States of America" pageId="2" pageNumber="63">U.S.A.</collectingCountry>
with 94% coverage (
<accessionNumber box="[1036,1159,1207,1231]" httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/MW443028" pageId="2" pageNumber="63">MW443028</accessionNumber>
–
<accessionNumber box="[1172,1295,1207,1231]" httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/MW443030" pageId="2" pageNumber="63">MW443030</accessionNumber>
:
<bibRefCitation author="Ksepka, S. P. &amp; Bullard, S. A." pageId="2" pageNumber="63" pagination="541 - 551" refId="ref6061" refString="Ksepka, S. P. and Bullard, S. A. 2021. Morphology, phylogenetics and pathology of &quot; red sore disease &quot; (coinfection by Epistylis cf. wuhanensis and Aeromonas hydrophila) on sportfishes from reservoirs in the South-Eastern United States. Journal of Fish Diseases 44: 541 - 551." type="journal article" year="2021">Ksepka and Bullard 2021</bibRefCitation>
). The closest hits to the newly generated sequence of ITS1-5.8S-ITS2 (
<accessionNumber box="[1097,1205,1271,1295]" httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/LC570273" pageId="2" pageNumber="63">LC570273</accessionNumber>
) is also
<taxonomicName authorityName="Wang, Zhou, Guo, and Gu" authorityYear="2017" box="[1288,1431,1271,1295]" class="Oligohymenophorea" family="Epistylididae" genus="Epistylis" kingdom="Chromista" order="Peritrichida" pageId="2" pageNumber="63" phylum="Ciliophora" rank="species" species="wuhanensis">
<emphasis box="[1288,1431,1271,1295]" italics="true" pageId="2" pageNumber="63">E. wuhanensis</emphasis>
</taxonomicName>
(
<accessionNumber box="[827,940,1304,1328]" httpUri="http://www.ncbi.nlm.nih.gov/nucleotide/KU869710" pageId="2" pageNumber="63">KU869710</accessionNumber>
,
<bibRefCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." box="[951,1126,1304,1329]" pageId="2" pageNumber="63" pagination="394 - 406" refId="ref7636" refString="Wang, Z., Zhou, T., Guo, Q., and Gu, Z. 2017. Description of a new freshwater ciliate Epistylis wuhanensis n. sp. (Ciliophora, Peritrichia) from China, with a focus on phylogenetic relationships within family Epistylididae. Journal of Eukaryotic Microbiology 64: 394 - 406." type="journal article" year="2017">Wang et al. 2017</bibRefCitation>
, 99.79% similarity with 100% coverage), and the newly determined ITS1 sequence showed only one base deletion.
</paragraph>
</subSubSection>
<subSubSection lastPageId="6" lastPageNumber="67" pageId="2" pageNumber="63" type="discussion">
<paragraph blockId="2.[820,1432,198,2077]" lastBlockId="6.[156,767,198,352]" lastPageId="6" lastPageNumber="67" pageId="2" pageNumber="63">
<emphasis bold="true" box="[846,945,1402,1426]" pageId="2" pageNumber="63">Remarks.</emphasis>
<taxonomicName authorityName="Wang, Zhou, Guo, and Gu" authorityYear="2017" box="[970,1174,1402,1426]" class="Oligohymenophorea" family="Epistylididae" genus="Epistylis" kingdom="Chromista" order="Peritrichida" pageId="2" pageNumber="63" phylum="Ciliophora" rank="species" species="wuhanensis">
<emphasis box="[970,1174,1402,1426]" italics="true" pageId="2" pageNumber="63">Epistylis wuhanensis</emphasis>
</taxonomicName>
was originally described as attached to the fins of the yellow catfish
<taxonomicName authority="(Richardson, 1846)" baseAuthorityName="Richardson" baseAuthorityYear="1846" class="Actinopterygii" family="Bagridae" genus="Tachysurus" kingdom="Animalia" order="Siluriformes" pageId="2" pageNumber="63" phylum="Chordata" rank="species" species="fulvidraco">
<emphasis italics="true" pageId="2" pageNumber="63">Tachysurus fulvidraco</emphasis>
(Richardson, 1846)
</taxonomicName>
(as
<taxonomicName baseAuthorityName="Richardson" baseAuthorityYear="1846" box="[1116,1345,1467,1491]" class="Actinopterygii" family="Bagridae" genus="Pelteobagrus" kingdom="Animalia" order="Siluriformes" pageId="2" pageNumber="63" phylum="Chordata" rank="species" species="fulvidraco">
<emphasis box="[1116,1345,1467,1491]" italics="true" pageId="2" pageNumber="63">Pelteobagrus fulvidraco</emphasis>
</taxonomicName>
) (
<taxonomicName class="Actinopterygii" kingdom="Animalia" order="Siluriformes" pageId="2" pageNumber="63" phylum="Chordata" rank="order">Siluriformes</taxonomicName>
:
<taxonomicName box="[904,997,1500,1524]" class="Actinopterygii" family="Bagridae" kingdom="Animalia" order="Siluriformes" pageId="2" pageNumber="63" phylum="Chordata" rank="family">Bagridae</taxonomicName>
), and the surface of the exposed neck and trunk of
<taxonomicName authorityName="Linnaeus" authorityYear="1758" box="[910,1041,1533,1556]" class="Hexanauplia" family="Lernaeidae" genus="Lernaea" kingdom="Animalia" order="Cyclopoida" pageId="2" pageNumber="63" phylum="Arthropoda" rank="species" species="cyprinacea">
<emphasis box="[910,1041,1533,1556]" italics="true" pageId="2" pageNumber="63">L. cyprinacea</emphasis>
</taxonomicName>
, parasitizing the bluegill
<taxonomicName authority="Rafinesque, 1819" authorityName="Rafinesque" authorityYear="1819" class="Actinopterygii" family="Centrarchidae" genus="Lepomis" kingdom="Animalia" order="Perciformes" pageId="2" pageNumber="63" phylum="Chordata" rank="species" species="macrochirus">
<emphasis italics="true" pageId="2" pageNumber="63">Lepomis macrochirus</emphasis>
Rafinesque, 1819
</taxonomicName>
(
<taxonomicName box="[1107,1232,1565,1589]" class="Actinopterygii" kingdom="Animalia" order="Perciformes" pageId="2" pageNumber="63" phylum="Chordata" rank="order">Perciformes</taxonomicName>
:
<taxonomicName box="[1247,1392,1565,1589]" class="Actinopterygii" family="Centrarchidae" kingdom="Animalia" order="Perciformes" pageId="2" pageNumber="63" phylum="Chordata" rank="family">Centrarchidae</taxonomicName>
) in
<collectingRegion box="[821,980,1597,1621]" country="China" name="Hubei" pageId="2" pageNumber="63">Hubei Province</collectingRegion>
,
<collectingCountry box="[989,1051,1597,1621]" name="China" pageId="2" pageNumber="63">China</collectingCountry>
(
<bibRefCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." box="[1064,1237,1597,1621]" pageId="2" pageNumber="63" pagination="394 - 406" refId="ref7636" refString="Wang, Z., Zhou, T., Guo, Q., and Gu, Z. 2017. Description of a new freshwater ciliate Epistylis wuhanensis n. sp. (Ciliophora, Peritrichia) from China, with a focus on phylogenetic relationships within family Epistylididae. Journal of Eukaryotic Microbiology 64: 394 - 406." type="journal article" year="2017">Wang et al. 2017</bibRefCitation>
). On newly collect- ed specimens, the maximum number of zooids was 60 (vs.
<quantity box="[821,873,1662,1686]" metricMagnitude="-1" metricUnit="m" metricValue="7.62" pageId="2" pageNumber="63" unit="in" value="30.0">30 in</quantity>
<bibRefCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." box="[881,1057,1662,1687]" pageId="2" pageNumber="63" pagination="394 - 406" refId="ref7636" refString="Wang, Z., Zhou, T., Guo, Q., and Gu, Z. 2017. Description of a new freshwater ciliate Epistylis wuhanensis n. sp. (Ciliophora, Peritrichia) from China, with a focus on phylogenetic relationships within family Epistylididae. Journal of Eukaryotic Microbiology 64: 394 - 406." type="journal article" year="2017">Wang et al. 2017</bibRefCitation>
); fully expanded zooids
<emphasis box="[1307,1376,1663,1686]" italics="true" pageId="2" pageNumber="63">in vivo</emphasis>
were slightly longer than those in the aforementioned records [163.1–220.3 µm (this study) vs. 90.2–175.4 µm (
<bibRefCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." pageId="2" pageNumber="63" pagination="394 - 406" refId="ref7636" refString="Wang, Z., Zhou, T., Guo, Q., and Gu, Z. 2017. Description of a new freshwater ciliate Epistylis wuhanensis n. sp. (Ciliophora, Peritrichia) from China, with a focus on phylogenetic relationships within family Epistylididae. Journal of Eukaryotic Microbiology 64: 394 - 406." type="journal article" year="2017">Wang et al. 2017</bibRefCitation>
)]. Zooids in half of the living specimens were the widest at the peristomial lip, but those of the other half and fixed specimens were the widest at their mid-point. However, most measurements and morphological characteristics (the composition of the infraciliature; the position of the contractile vacuole; the form of the peristomial disk and lip; and the shape and position of the macronucleus) of the specimens collected in the present study are closely resemble to the description of
<bibRefCitation author="Wang, Z. &amp; Zhou, T. &amp; Guo, Q. &amp; Gu, Z." box="[947,1138,2020,2045]" pageId="2" pageNumber="63" pagination="394 - 406" refId="ref7636" refString="Wang, Z., Zhou, T., Guo, Q., and Gu, Z. 2017. Description of a new freshwater ciliate Epistylis wuhanensis n. sp. (Ciliophora, Peritrichia) from China, with a focus on phylogenetic relationships within family Epistylididae. Journal of Eukaryotic Microbiology 64: 394 - 406." type="journal article" year="2017">Wang et al. (2017)</bibRefCitation>
, partial 18S rDNA sequence was identical, and ITS1-5.8S-ITS2 sequence was also closely similar to the latter. The minor morphological differences of the specimens and the one base deletion of the newly determined ITS1 sequence are considered to be intraspecific variations, thus, the newly collected specimens were identified as a new country-specific record for
<emphasis box="[494,641,328,352]" italics="true" pageId="6" pageNumber="67">
<taxonomicName authorityName="Wang, Zhou, Guo, and Gu" authorityYear="2017" box="[494,637,328,352]" class="Oligohymenophorea" family="Epistylididae" genus="Epistylis" kingdom="Chromista" order="Peritrichida" pageId="6" pageNumber="67" phylum="Ciliophora" rank="species" species="wuhanensis">E. wuhanensis</taxonomicName>
.
</emphasis>
</paragraph>
</subSubSection>
</treatment>
</document>
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