treatments-xml/data/0E/0F/87/0E0F87F99B71FFC82F951E34BF43FE53.xml
2024-06-21 12:29:25 +02:00

296 lines
57 KiB
XML
Raw Blame History

This file contains ambiguous Unicode characters

This file contains Unicode characters that might be confused with other characters. If you think that this is intentional, you can safely ignore this warning. Use the Escape button to reveal them.

<document id="BB14943B9522288ECAEA438640C4C9C2" ID-CLB-Dataset="25804" ID-DOI="10.11646/zootaxa.4664.2.3" ID-GBIF-Dataset="0446cda6-a718-4458-8777-d5e8c433b8bf" ID-ISSN="1175-5326" ID-Zenodo-Dep="3384530" ID-ZooBank="AD304BBB-FFED-4AFD-940C-F0DAA8C156BE" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="existingObjects,plazi" IM.taxonomicNames_approvedBy="felipe" checkinTime="1567495549173" checkinUser="plazi" docAuthor="Wang, Congcong, Qu, Zhishuai &amp; Hu, Xiaozhong" docDate="2019" docId="0E0F87F99B71FFC82F951E34BF43FE53" docLanguage="en" docName="zootaxa.4664.2.3.pdf" docOrigin="Zootaxa 4664 (2)" docStyle="DocumentStyle:647186512141C8FC8976D5BCC54AEB7D.9:Zootaxa.2013-.journal_article" docStyleId="647186512141C8FC8976D5BCC54AEB7D" docStyleName="Zootaxa.2013-.journal_article" docStyleVersion="9" docTitle="Chlamydodon pararoseus Wang &amp; Qu &amp; Hu 2019, sp. n." docType="treatment" docUuid="7625A4BC-96E8-4ADA-8E83-89849A844199" docUuidSource="ZooBank" docVersion="10" lastPageNumber="210" masterDocId="F236FF819B73FFCC2F021B03BB00FFA4" masterDocTitle="Morphology and SSU rDNA sequences of four cyrtophorian ciliates from China, with description of a new species (Protista, Ciliophora, Phyllopharyngea)" masterLastPageNumber="220" masterPageNumber="206" pageNumber="208" updateTime="1698754362358" updateUser="ExternalLinkService">
<mods:mods id="D6EBA38397B0E2E1BACA07B674001BE8" xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo id="EC9BF5FE2F8B7F239BBA3DCE763EB8D7">
<mods:title id="A01F28D260BCA15C8BD2C21DCB8E42C4">Morphology and SSU rDNA sequences of four cyrtophorian ciliates from China, with description of a new species (Protista, Ciliophora, Phyllopharyngea)</mods:title>
</mods:titleInfo>
<mods:name id="B8FA3E4DD625D64DF32656EB0964C3C2" type="personal">
<mods:role id="3B085663BABBBEFAD6884C0DADDCDD1E">
<mods:roleTerm id="2C859998C7932D95F1A2B8A656F9B831">Author</mods:roleTerm>
</mods:role>
<mods:namePart id="9AEFB0E342E00DAC69A778C96EA60EC5">Wang, Congcong</mods:namePart>
</mods:name>
<mods:name id="4986256B47648BAA1717E4ED6F8F9F3D" type="personal">
<mods:role id="359BCB206EDB9226FA4A45649E11E707">
<mods:roleTerm id="C5FCE98BE5735D8D6BC0584C6FCE0C1B">Author</mods:roleTerm>
</mods:role>
<mods:namePart id="0C172E69FF559917FE73DC9FE3B2748C">Qu, Zhishuai</mods:namePart>
</mods:name>
<mods:name id="F6B195D9A4C2321ED798F969BB70A965" type="personal">
<mods:role id="B7505C364C64D14674A28441DEC2C85A">
<mods:roleTerm id="E49FBDF7C6C0B30B75609E822FF3F610">Author</mods:roleTerm>
</mods:role>
<mods:namePart id="1585C20DD0EE6B9B058ADCFAC088F8F6">Hu, Xiaozhong</mods:namePart>
</mods:name>
<mods:typeOfResource id="C9A98BB5400CFC26EE3231F2A3FC62FF">text</mods:typeOfResource>
<mods:relatedItem id="7590B8AF9A0581FE2CC81DCD95F876AC" type="host">
<mods:titleInfo id="71AD213F1BA9B2212AB53EB5486F423F">
<mods:title id="5CF7E78A7F31462955C433F3758541FD">Zootaxa</mods:title>
</mods:titleInfo>
<mods:part id="3CE4241051A379A6E6D6EDFAC03A38E3">
<mods:date id="45DFA56D48B4B9551992F04D0628D52C">2019</mods:date>
<mods:detail id="C09B29A4D629AFDAF51CBEAD03DB2488" type="pubDate">
<mods:number id="F1CD2F8128BEEDCB0AF5AB531499D1F5">2019-09-03</mods:number>
</mods:detail>
<mods:detail id="433EFEF9E62185453E96800D658DA57D" type="volume">
<mods:number id="2E36CF842C95135EBD6F088C2BBEC465">4664</mods:number>
</mods:detail>
<mods:detail id="90B303CCD62ADC18CC4F58424B36BE86" type="issue">
<mods:number id="27EC4A4A8AB8CFC2F466C287654E8BD4">2</mods:number>
</mods:detail>
<mods:extent id="E4121297E90D8AD86763DFFC72FF3B52" unit="page">
<mods:start id="AA592F84F2E8A3D12760A856E7A63B05">206</mods:start>
<mods:end id="E3A8AE5CF2B3E4769CA19C0DD7A9CE89">220</mods:end>
</mods:extent>
</mods:part>
</mods:relatedItem>
<mods:classification id="E790E3C645335EA9C84F3593C5D6806E">journal article</mods:classification>
<mods:identifier id="EC556F31CC7BA6BC30C25E12898B1881" type="CLB-Dataset">25804</mods:identifier>
<mods:identifier id="A6347DD354EDDFB6F8F56DE236DAE79A" type="DOI">10.11646/zootaxa.4664.2.3</mods:identifier>
<mods:identifier id="2936E689E013B02D523955181151079C" type="GBIF-Dataset">0446cda6-a718-4458-8777-d5e8c433b8bf</mods:identifier>
<mods:identifier id="F22CD0319C9BA89A9C54E4FF591284BC" type="ISSN">1175-5326</mods:identifier>
<mods:identifier id="34AE744B4DBC1E3D158BD74831644343" type="Zenodo-Dep">3384530</mods:identifier>
<mods:identifier id="AD3637F907D50000F1421C47F6E5DB57" type="ZooBank">AD304BBB-FFED-4AFD-940C-F0DAA8C156BE</mods:identifier>
</mods:mods>
<treatment id="0E0F87F99B71FFC82F951E34BF43FE53" ID-GBIF-Taxon="159359192" LSID="urn:lsid:zoobank.org:act:7625A4BC-96E8-4ADA-8E83-89849A844199" httpUri="http://treatment.plazi.org/id/0E0F87F99B71FFC82F951E34BF43FE53" lastPageId="4" lastPageNumber="210" pageId="2" pageNumber="208">
<subSubSection id="CEBC65649B71FFCE2F951E34B917FAF5" box="[151,535,1335,1362]" pageId="2" pageNumber="208" type="nomenclature">
<paragraph id="861936EF9B71FFCE2F951E34B917FAF5" blockId="2.[151,997,1335,1427]" box="[151,535,1335,1362]" pageId="2" pageNumber="208">
<heading id="DD5181839B71FFCE2F951E34B917FAF5" bold="true" box="[151,535,1335,1362]" fontSize="11" level="1" pageId="2" pageNumber="208" reason="1">
<emphasis id="B4D2EAFD9B71FFCE2F951E34B917FAF5" bold="true" box="[151,535,1335,1362]" pageId="2" pageNumber="208">
<taxonomicName id="41A64D6C9B71FFCE2F951E34BACEFAF6" authority="Wang &amp; Qu &amp; Hu, 2019" authorityName="Wang &amp; Qu &amp; Hu" authorityYear="2019" box="[151,462,1335,1362]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="2" pageNumber="208" phylum="Ciliophora" rank="species" species="pararoseus" status="sp. nov.">
<emphasis id="B4D2EAFD9B71FFCE2F951E34BACEFAF6" bold="true" box="[151,462,1335,1362]" italics="true" pageId="2" pageNumber="208">Chlamydodon pararoseus</emphasis>
</taxonomicName>
<taxonomicNameLabel id="AFE157869B71FFCE2ED71E34B917FAF5" box="[469,535,1335,1361]" pageId="2" pageNumber="208" rank="species">sp. n.</taxonomicNameLabel>
</emphasis>
</heading>
</paragraph>
</subSubSection>
<subSubSection id="CEBC65649B71FFCE2F951E58B8E5FA36" pageId="2" pageNumber="208" type="description">
<paragraph id="861936EF9B71FFCE2F951E58BA6FFAD1" blockId="2.[151,997,1335,1427]" box="[151,367,1371,1397]" pageId="2" pageNumber="208">
(
<figureCitation id="1E9D2A6A9B71FFCE2F9D1E58BBE8FAD1" box="[159,232,1371,1397]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="2" pageNumber="208">Figs 2</figureCitation>
,
<figureCitation id="1E9D2A6A9B71FFCE2FF61E58BA04FAD1" box="[244,260,1371,1397]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="2" pageNumber="208">3</figureCitation>
;
<tableCitation id="CB2403549B71FFCE2E131E58BA63FAD1" box="[273,355,1371,1397]" captionStart="TABLE 1" captionStartId="6.[151,239,370,394]" captionTargetPageId="6" captionText="TABLE 1. Morphometric data of Chlamydodon pararoseus sp. n. (upper lines) and C. bourlandi (lower lines) based on protargol-stained specimens." httpUri="http://table.plazi.org/id/D2D966679B75FFCA2F951A71BACAFE0B" pageId="2" pageNumber="208" tableUuid="D2D966679B75FFCA2F951A71BACAFE0B">Table 1</tableCitation>
)
</paragraph>
<paragraph id="861936EF9B71FFCE2F951E7AB8E5FA36" blockId="2.[151,997,1335,1427]" box="[151,997,1400,1427]" pageId="2" pageNumber="208">
<uri id="F2373AED9B71FFCE2F951E7AB8E5FA36" box="[151,997,1400,1427]" pageId="2" pageNumber="208">
urn: lsid: zoobank.org: act:
<uuid id="F2000C3A9B71FFCE2ECE1E7BB8E5FA36" box="[460,997,1400,1426]" pageId="2" pageNumber="208">CABCE2B6-C7B8-4E64-88F0-4DF76763492F</uuid>
</uri>
</paragraph>
</subSubSection>
<subSubSection id="CEBC65649B71FFC82F951EC3BF43FE53" lastPageId="4" lastPageNumber="210" pageId="2" pageNumber="208" type="materials_examined">
<paragraph id="861936EF9B71FFCE2F951EC3B978F9E2" blockId="2.[151,1437,1472,2039]" pageId="2" pageNumber="208">
<emphasis id="B4D2EAFD9B71FFCE2F951EC3BA12FA7F" bold="true" box="[151,274,1472,1499]" pageId="2" pageNumber="208">Diagnosis.</emphasis>
Cell 70100 μm long in vivo, broadly bean-shaped to kidney-shaped in outline; cross-striated band complete; two yellowish brown to reddish pigment spots in anterior-left and posterior-right of cell, respectively; 4451 somatic kineties including 2328 right, 1619 left, and four postoral kineties; 1114 nematodesmal rods; two to four terminal fragments. Marine habitat.
</paragraph>
<paragraph id="861936EF9B71FFCE2FC51D53BA8CF92A" blockId="2.[151,1437,1472,2039]" pageId="2" pageNumber="208">
Zoobank registration of
<taxonomicName id="41A64D6C9B71FFCE2ED61D53B9F4F9CE" authority="Wang &amp; Qu &amp; Hu, 2019" authorityName="Wang &amp; Qu &amp; Hu" authorityYear="2019" box="[468,756,1616,1642]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="2" pageNumber="208" phylum="Ciliophora" rank="species" species="pararoseus" status="sp. nov.">
<emphasis id="B4D2EAFD9B71FFCE2ED61D53B9F4F9CE" box="[468,756,1616,1642]" italics="true" pageId="2" pageNumber="208">Chlamydodon pararoseus</emphasis>
</taxonomicName>
<emphasis id="B4D2EAFD9B71FFCE2DF91D52B839F9CF" bold="true" box="[763,825,1617,1643]" pageId="2" pageNumber="208">
<taxonomicNameLabel id="AFE157869B71FFCE2DF91D52B839F9CF" box="[763,825,1617,1643]" pageId="2" pageNumber="208" rank="species">sp. n.</taxonomicNameLabel>
</emphasis>
:
<uri id="F2373AED9B71FFCE2C451D52BA8CF92A" pageId="2" pageNumber="208">
urn: lsid: zoobank.org: act:
<uuid id="F2000C3A9B71FFCE2B7A1D53BA8CF92A" pageId="2" pageNumber="208">CABCE2B6-C7B8-4E64- 88F0-4DF76763492F</uuid>
</uri>
</paragraph>
<paragraph id="861936EF9B71FFCE2FC51D9ABE7EF917" blockId="2.[151,1437,1472,2039]" box="[199,1406,1688,1715]" pageId="2" pageNumber="208">
<materialsCitation id="36CE3CB29B71FFCE2FC51D9ABE7AF917" ID-GBIF-Occurrence="2382361215" box="[199,1402,1688,1715]" country="China" county="Qingdao" latitude="36.05" location="Seawater" longLatPrecision="1190" longitude="120.35" municipality="Taiping Bay" pageId="2" pageNumber="208" specimenCount="1" typeStatus="holotype">
<emphasis id="B4D2EAFD9B71FFCE2FC51D9ABA66F917" bold="true" box="[199,358,1688,1715]" pageId="2" pageNumber="208">
<typeStatus id="591D884D9B71FFCE2FC51D9ABA02F917" box="[199,258,1689,1715]" pageId="2" pageNumber="208">Type</typeStatus>
locality.
</emphasis>
<location id="837960349B71FFCE2E6E1D9ABAD3F917" LSID="urn:lsid:plazi:treatment:0E0F87F99B71FFC82F951E34BF43FE53:837960349B71FFCE2E6E1D9ABAD3F917" box="[364,467,1689,1715]" country="China" county="Qingdao" latitude="36.05" longLatPrecision="1190" longitude="120.35" municipality="Taiping Bay" name="Seawater" pageId="2" pageNumber="208">Seawater</location>
in a rocky tidal pool in
<collectingMunicipality id="667DAC959B71FFCE2DDD1D9BB869F917" box="[735,873,1688,1715]" pageId="2" pageNumber="208">Taiping Bay</collectingMunicipality>
,
<collectingCounty id="6F784E639B71FFCE2C761D9BB8D7F916" box="[884,983,1688,1714]" pageId="2" pageNumber="208">Qingdao</collectingCounty>
, northern
<collectingCountry id="FEB1767F9B71FFCE2B4A1D9BBF8BF916" box="[1096,1163,1688,1714]" name="China" pageId="2" pageNumber="208">China</collectingCountry>
(
<geoCoordinate id="E39250289B71FFCE2B991D9BBFFBF916" box="[1179,1275,1688,1714]" degrees="36" direction="north" minutes="03" orientation="latitude" pageId="2" pageNumber="208" precision="925" value="36.05">36°03'N</geoCoordinate>
,
<geoCoordinate id="E39250289B71FFCE2A041D9ABE72F917" box="[1286,1394,1689,1715]" degrees="120" direction="east" minutes="21" orientation="longitude" pageId="2" pageNumber="208" precision="925" value="120.35">120°21'E</geoCoordinate>
)
</materialsCitation>
.
</paragraph>
<paragraph id="861936EF9B71FFCE2FC51DBEB981F8BB" blockId="2.[151,1437,1472,2039]" pageId="2" pageNumber="208">
<emphasis id="B4D2EAFD9B71FFCE2FC51DBEBA56F973" bold="true" box="[199,342,1724,1751]" pageId="2" pageNumber="208">Type slides.</emphasis>
A protargol slide including the
<typeStatus id="591D884D9B71FFCE2DD41DBFB838F972" box="[726,824,1724,1750]" pageId="2" pageNumber="208" type="holotype">holotype</typeStatus>
specimen (
<figureCitation id="1E9D2A6A9B71FFCE2CC31DBFBF3DF973" box="[961,1085,1724,1751]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="2" pageNumber="208">Fig. 2E, F</figureCitation>
) and another slide with
<typeStatus id="591D884D9B71FFCE2A611DBEBBC7F95F" pageId="2" pageNumber="208" type="paratype">paratype</typeStatus>
specimens are deposited in the Laboratory of Protozoology, OUC,
<collectingCountry id="FEB1767F9B71FFCE2CEA1DE3BF2EF95E" box="[1000,1070,1760,1786]" name="China" pageId="2" pageNumber="208">China</collectingCountry>
, with the registration numbers: WCC2018041901-1 &amp; WCC2018041901-2
</paragraph>
<paragraph id="861936EF9B71FFCE2FC51C2ABBE6F82F" blockId="2.[151,1437,1472,2039]" pageId="2" pageNumber="208">
<emphasis id="B4D2EAFD9B71FFCE2FC51C2ABA4EF8E7" bold="true" box="[199,334,1833,1859]" pageId="2" pageNumber="208">Etymology.</emphasis>
The specific epithet “
<taxonomicName id="41A64D6C9B71FFCE2D401C2AB9BFF8E6" authorityName="Wang &amp; Qu &amp; Hu" authorityYear="2019" box="[578,703,1833,1858]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="2" pageNumber="208" phylum="Ciliophora" rank="species" species="pararoseus">
<emphasis id="B4D2EAFD9B71FFCE2D401C2AB9BFF8E6" box="[578,703,1833,1858]" italics="true" pageId="2" pageNumber="208">pararoseus</emphasis>
</taxonomicName>
” is derived by adding the Greek prefix “para-” (meaning “beside, at, along, during”) to the epithet “
<taxonomicName id="41A64D6C9B71FFCE2D221C4EB968F8C2" authorityName="Dragesco" authorityYear="1965" box="[544,616,1869,1894]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="2" pageNumber="208" phylum="Ciliophora" rank="species" species="roseus">
<emphasis id="B4D2EAFD9B71FFCE2D221C4EB968F8C2" box="[544,616,1869,1894]" italics="true" pageId="2" pageNumber="208">roseus</emphasis>
</taxonomicName>
,” in recognition that this species morphologically closely resembles
<taxonomicName id="41A64D6C9B71FFCE2A811C4EBBDFF82E" authorityName="Dragesco" authorityYear="1965" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="2" pageNumber="208" phylum="Ciliophora" rank="species" species="roseus">
<emphasis id="B4D2EAFD9B71FFCE2A811C4EBBDFF82E" italics="true" pageId="2" pageNumber="208">C. roseus</emphasis>
</taxonomicName>
.
</paragraph>
<paragraph id="861936EF9B71FFCF2FC51C97B8FFFE53" blockId="2.[151,1437,1472,2039]" lastBlockId="3.[151,1437,153,755]" lastPageId="3" lastPageNumber="209" pageId="2" pageNumber="208">
<emphasis id="B4D2EAFD9B71FFCE2FC51C97B90AF80B" bold="true" box="[199,522,1940,1967]" pageId="2" pageNumber="208">Morphological description.</emphasis>
Size 70100 ×
<quantity id="415E9B0A9B71FFCE2DBA1C97B82AF80B" box="[696,810,1940,1967]" metricMagnitude="-2" metricUnit="m" metricValue="5.5" metricValueMax="6.0" metricValueMin="5.0" pageId="2" pageNumber="208" unit="mm" value="55.0" valueMax="60.0" valueMin="50.0">5060 µm</quantity>
in vivo, and 6590 ×
<quantity id="415E9B0A9B71FFCE2B1F1C97BF90F80B" box="[1053,1168,1940,1967]" metricMagnitude="-2" metricUnit="m" metricValue="4.0" metricValueMax="5.0" metricValueMin="3.0" pageId="2" pageNumber="208" unit="mm" value="40.0" valueMax="50.0" valueMin="30.0">3050 µm</quantity>
after protargol staining. Body broadly bean-shaped or kidney-shaped with both ends evenly rounded (
<figureCitation id="1E9D2A6A9B71FFCE2B091CBBBF6AF877" box="[1035,1130,1976,2003]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="2" pageNumber="208">Figs 2A</figureCitation>
,
<figureCitation id="1E9D2A6A9B71FFCE2B751CBABF9DF877" box="[1143,1181,1977,2003]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="2" pageNumber="208">3A</figureCitation>
); right margin slightly convex and left one somewhat concave (
<figureCitation id="1E9D2A6A9B71FFCE2D6B1CDFB9C8F853" box="[617,712,2012,2039]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="2" pageNumber="208">Figs 2A</figureCitation>
,
<figureCitation id="1E9D2A6A9B71FFCE2DD41CDEB81BF853" box="[726,795,2013,2039]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="2" pageNumber="208">3D, F</figureCitation>
); cell dorsoventrally compressed with a width-thickness ratio of about 4:1. Cross-striated band complete, 12 μm wide, comprising about 140 C-shaped clasps. Two small yellowish brown to reddish pigment spots positioned in anterior-left and posterior-right of cell, respectively (
<figureCitation id="1E9D2A6A9B70FFCF2A681BBEBBA5FF5F" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="3" pageNumber="209">Figs 2</figureCitation>
AC, 3AF). Many bright brown granules (about 0.5 μm across) distributed mainly along ciliary rows. Cytoplasm contains many undigested reddish to brown pigments or ingested particles (
<quantity id="415E9B0A9B70FFCF2CE01A06BF3AFEBB" box="[994,1082,261,287]" metricMagnitude="-3" metricUnit="m" metricValue="6.0" metricValueMax="8.0" metricValueMin="4.0" pageId="3" pageNumber="209" unit="mm" value="6.0" valueMax="8.0" valueMin="4.0">48 µm</quantity>
across;
<figureCitation id="1E9D2A6A9B70FFCF2B941A06BFD5FEBB" box="[1174,1237,261,287]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="3" pageNumber="209">Fig. 3</figureCitation>
AC). Cytostome elliptical, located at approximately anterior 30% of cell, about 16 μm long; cyrtos composed of 1114 nematodes- mal rods, each about 25 μm long (
<figureCitation id="1E9D2A6A9B70FFCF2D101A4EB96FFEC3" box="[530,623,333,359]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="3" pageNumber="209">Figs 2D</figureCitation>
,
<figureCitation id="1E9D2A6A9B70FFCF2D781A4EB9BEFEC3" box="[634,702,333,359]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="3" pageNumber="209">3A, E</figureCitation>
). About 2030 contractile vacuoles (24 μm across, counted from three live individuals) irregularly distributed (
<figureCitation id="1E9D2A6A9B70FFCF2D941A72B9F3FE2F" box="[662,755,369,395]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="3" pageNumber="209">Figs 2A</figureCitation>
,
<figureCitation id="1E9D2A6A9B70FFCF2DFC1A72B824FE2F" box="[766,804,369,395]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="3" pageNumber="209">3A</figureCitation>
), with a contraction interval of about 4 seconds. A single ellipsoidal, heteromerous macronucleus, about 25 × 20 μm in vivo and after fluorescent staining, and about
<quantity id="415E9B0A9B70FFCF2A511A96BE9CFE0B" box="[1363,1436,405,431]" metricMagnitude="-2" metricUnit="m" metricValue="2.0" pageId="3" pageNumber="209" unit="mm" value="20.0">20 µm</quantity>
across after protargol staining, located in mid-body (
<figureCitation id="1E9D2A6A9B70FFCF2DE11ABAB85CFE77" box="[739,860,441,467]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="3" pageNumber="209">Figs 2A, F</figureCitation>
,
<figureCitation id="1E9D2A6A9B70FFCF2C651ABAB8B0FE77" box="[871,944,441,467]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="3" pageNumber="209">3A, G</figureCitation>
). Micronucleus not detected. Cells crawl on the substrate or swim in water by rotating around the longitudinal axis of cell.
</paragraph>
<paragraph id="861936EF9B70FFCF2FC51902BFE3FD57" blockId="3.[151,1437,153,755]" pageId="3" pageNumber="209">
Cilia about 5 μm long in vivo. In total 4451 somatic kineties including 2328 right, 1619 left, and invariably four postoral kineties; somatic kineties densely arranged, mostly confined to region surrounded by cross-striated band. Anterior ends of right kineties extend beyond level of cytostome and bend to left; outermost 1012 right kineties usually extend onto dorsal surface and the innermost one is interrupted by oral kineties. Left kineties extend sub-apically and form a hook-like suture with right kineties (
<figureCitation id="1E9D2A6A9B70FFCF2D981992B811FD0F" box="[666,785,657,683]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="3" pageNumber="209">Figs 2E, G</figureCitation>
,
<figureCitation id="1E9D2A6A9B70FFCF2C191992B840FD0F" box="[795,832,657,683]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="3" pageNumber="209">3H</figureCitation>
). A sub-equatorial fossa with four or five kineties on the ventral side (
<figureCitation id="1E9D2A6A9B70FFCF2E2619B6BA7CFD6B" box="[292,380,693,719]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="3" pageNumber="209">Figs 2E</figureCitation>
,
<figureCitation id="1E9D2A6A9B70FFCF2E8519B6BAC8FD6B" box="[391,456,693,719]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="3" pageNumber="209">3B, L</figureCitation>
). Two to four terminal fragments, each composed of 415 basal bodies, arranged in a row adjacent to the outermost right kinety on dorsal side (
<figureCitation id="1E9D2A6A9B70FFCF2DDE19DAB831FD57" box="[732,817,729,755]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="3" pageNumber="209">Figs 2F</figureCitation>
,
<figureCitation id="1E9D2A6A9B70FFCF2C3E19DAB861FD57" box="[828,865,729,755]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="3" pageNumber="209">3K</figureCitation>
). Equatorial fragment not detected.
</paragraph>
<caption id="D2D966679B70FFCF2F951C07BA54F851" httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="3" pageNumber="209" startId="3.[151,250,1796,1821]" targetBox="[230,1377,769,1761]" targetPageId="3">
<paragraph id="861936EF9B70FFCF2F951C07BA54F851" blockId="3.[151,1437,1796,2037]" pageId="3" pageNumber="209">
<emphasis id="B4D2EAFD9B70FFCF2F951C07BA14F8B9" bold="true" box="[151,276,1796,1821]" pageId="3" pageNumber="209">FIGURE 2.</emphasis>
Drawings of
<taxonomicName id="41A64D6C9B70FFCF2EA31C07B9A8F8B8" authority="Wang &amp; Qu &amp; Hu, 2019" authorityName="Wang &amp; Qu &amp; Hu" authorityYear="2019" box="[417,680,1796,1820]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="3" pageNumber="209" phylum="Ciliophora" rank="species" species="pararoseus" status="sp. nov.">
<emphasis id="B4D2EAFD9B70FFCF2EA31C07B9A8F8B8" box="[417,680,1796,1820]" italics="true" pageId="3" pageNumber="209">Chlamydodon pararoseus</emphasis>
</taxonomicName>
<emphasis id="B4D2EAFD9B70FFCF2DAD1C06B9E9F8B9" bold="true" box="[687,745,1797,1821]" pageId="3" pageNumber="209">
<taxonomicNameLabel id="AFE157869B70FFCF2DAD1C06B9E9F8B9" box="[687,745,1797,1821]" pageId="3" pageNumber="209" rank="species">sp. n.</taxonomicNameLabel>
</emphasis>
from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronu-cleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm.
</paragraph>
</caption>
<paragraph id="861936EF9B77FFC82FC51B9AB809FEBB" blockId="4.[151,1436,153,503]" pageId="4" pageNumber="210">
Three oral kineties obliquely oriented: two circumoral kineties parallel and closely arranged to each other, the outer row longer than the inner one; preoral kinety located in anterior-left of circumoral kineties (
<figureCitation id="1E9D2A6A9B77FFC82BEB1BBEBE68FF73" box="[1257,1384,189,215]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="4" pageNumber="210">Figs 2E, G</figureCitation>
,
<figureCitation id="1E9D2A6A9B77FFC82A771BBEBE8EFF73" box="[1397,1422,189,215]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="4" pageNumber="210">
<date id="F218102F9B77FFC82A771BBEBE8EFF73" box="[1397,1422,189,215]" pageId="4" pageNumber="210">3I</date>
</figureCitation>
). One circle of barren kinetosomes (corresponding to position of tips of nematodesmal rods) observed surrounding cytostome in protargol-stained specimens (
<figureCitation id="1E9D2A6A9B77FFC82D751A06B9D5FEBB" box="[631,725,261,287]" captionStart="FIGURE 2" captionStartId="3.[151,250,1796,1821]" captionTargetBox="[230,1377,769,1761]" captionTargetId="figure@3.[207,1380,769,1776]" captionTargetPageId="3" captionText="FIGURE 2. Drawings of Chlamydodon pararoseus sp. n. from life (AD) and after protargol staining (EG). A. Ventral side of a representative individual. Dorsal (B) and ventral (C) views of a compressed individual, arrows denote the cross-striated band (CSB) and the arrowheads mark pigment spots. D. Cyrtos. E, F. Ventral (E) and dorsal (F) views of the same holotype specimen, arrow indicates the sub-equatorial fossa. G. Ventral view of anterior cell, arrow marks the suture, arrowheads denote barren kinetosomes. Abbreviations: Co—circumoral kineties; CSB—cross-striated band; LK—left kineties; Ma—macronucleus; NR—nematodesmal rods; PoK—postoral kineties; Pr—preoral kinety; RK—right kineties; TF—terminal fragments. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384534/files/figure.png" pageId="4" pageNumber="210">Figs 2G</figureCitation>
,
<figureCitation id="1E9D2A6A9B77FFC82DE21A06B9FCFEBB" box="[736,764,261,287]" captionStart="FIGURE 3" captionStartId="4.[151,250,1384,1409]" captionTargetBox="[151,1436,517,1365]" captionTargetId="figure@4.[151,1436,517,1365]" captionTargetPageId="4" captionText="FIGURE 3. Photomicrographs of Chlamydodon pararoseus sp. n. from life (A, D, and E by differential interference contrast, B, C, and F in bright field), after fluorescent staining (G), and after protargol staining (HL). A, B. Ventral views of slightly compressed individuals, arrow in (A) marks the cytostome, and arrowheads indicate contractile vacuoles, and arrows in (B) refer to the sub-equatorial fossa. C, E. Dorsal views, arrow marks the cytostome, and arrowheads mark the pigment spot. D, F. Ventral views of individuals showing variations in body shape. G. Macronucleus. H. Ventral ciliature. I. Oral structure. J. Barren kinetosomes in oral area. K. Terminal fragment. L. Sub-equatorial fossa after protargol staining.Abbreviations: Co—circumoral kineties; Ks—barren kinetosomes; Ma—macronucleus; Pr—preoral kinety; TF—terminal fragment. Scale bars: 40 μm." httpUri="https://zenodo.org/record/3384536/files/figure.png" pageId="4" pageNumber="210">3J</figureCitation>
).
</paragraph>
<paragraph id="861936EF9B77FFC82FC51A2BBF43FE53" blockId="4.[151,1436,153,503]" pageId="4" pageNumber="210">
<emphasis id="B4D2EAFD9B77FFC82FC51A2BB83BFEE7" bold="true" box="[199,827,296,323]" pageId="4" pageNumber="210">
<collectionCode id="E0B7AE2A9B77FFC82FC51A2BBBFAFEE7" box="[199,250,296,323]" country="0" httpUri="http://grbio.org/cool/fye2-vfes" name="Saratov State University" pageId="4" pageNumber="210">SSU</collectionCode>
rRNA gene sequence and phylogenetic position.
</emphasis>
The new
<collectionCode id="E0B7AE2A9B77FFC82CAE1A2AB8DFFEE7" box="[940,991,297,323]" country="0" httpUri="http://grbio.org/cool/fye2-vfes" name="Saratov State University" pageId="4" pageNumber="210">SSU</collectionCode>
rRNA gene sequence has the GenBank accession number, length, and G + C content of
<accessionNumber id="99F5AB0C9B77FFC82DA91A4EB82FFEC3" box="[683,815,333,359]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/MK882886" pageId="4" pageNumber="210">
<collectionCode id="E0B7AE2A9B77FFC82DA91A4EB9D9FEC3" box="[683,729,333,359]" country="Kenya" name="National Museum of Kenya" pageId="4" pageNumber="210">MK</collectionCode>
882886
</accessionNumber>
, 1,582 bp, and 45.64%, respectively. The closest related
<taxonomicName id="41A64D6C9B77FFC82F951A72BA34FE2F" authorityName="Ehrenberg" authorityYear="1835" box="[151,308,369,395]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="4" pageNumber="210" phylum="Ciliophora" rank="genus">
<emphasis id="B4D2EAFD9B77FFC82F951A72BA34FE2F" box="[151,308,369,395]" italics="true" pageId="4" pageNumber="210">Chlamydodon</emphasis>
</taxonomicName>
species are the Qingdao population of
<taxonomicName id="41A64D6C9B77FFC82DF91A72BF10FE2F" box="[763,1040,369,395]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="4" pageNumber="210" phylum="Ciliophora" rank="species" species="bourlandi">
<emphasis id="B4D2EAFD9B77FFC82DF91A72BF10FE2F" box="[763,1040,369,395]" italics="true" pageId="4" pageNumber="210">Chlamydodon bourlandi</emphasis>
</taxonomicName>
(
<accessionNumber id="99F5AB0C9B77FFC82B201A72BFA6FE2F" box="[1058,1190,369,395]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/MK882887" pageId="4" pageNumber="210" type="EnaNcbi">
<collectionCode id="E0B7AE2A9B77FFC82B201A72BF51FE2F" box="[1058,1105,369,395]" country="Kenya" name="National Museum of Kenya" pageId="4" pageNumber="210">MK</collectionCode>
882887
</accessionNumber>
) in present work and
<taxonomicName id="41A64D6C9B77FFC82F951A96BA19FE0B" authorityName="Kahl" authorityYear="1931" box="[151,281,405,431]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="4" pageNumber="210" phylum="Ciliophora" rank="species" species="obliquus">
<emphasis id="B4D2EAFD9B77FFC82F951A96BA19FE0B" box="[151,281,405,431]" italics="true" pageId="4" pageNumber="210">C. obliquus</emphasis>
</taxonomicName>
(
<accessionNumber id="99F5AB0C9B77FFC82E281A96BA9DFE0B" box="[298,413,405,431]" httpUri="https://www.ebi.ac.uk/ena/browser/api/embl/FJ998030" pageId="4" pageNumber="210">
<collectionCode id="E0B7AE2A9B77FFC82E281A96BA45FE0B" box="[298,325,405,431]" pageId="4" pageNumber="210">FJ</collectionCode>
998030
</accessionNumber>
, =
<taxonomicName id="41A64D6C9B77FFC82EC01A96B952FE0B" box="[450,594,405,431]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="4" pageNumber="210" phylum="Ciliophora" rank="species" species="bourlandi">
<emphasis id="B4D2EAFD9B77FFC82EC01A96B952FE0B" box="[450,594,405,431]" italics="true" pageId="4" pageNumber="210">C. bourlandi</emphasis>
</taxonomicName>
, as shown in
<bibRefCitation id="E2374B1E9B77FFC82DF31A96B8ADFE0B" author="Qu, Z. S. &amp; Li, L. F. &amp; Lin, X. F. &amp; Stoeck, T. &amp; Pan, H. B. &amp; Al-Rasheid, K. A. &amp; Song, W. B." box="[753,941,405,431]" pageId="4" pageNumber="210" pagination="497 - 511" refId="ref9680" refString="Qu, Z. S., Li, L. F., Lin, X. F., Stoeck, T., Pan, H. B., Al-Rasheid, K. A. &amp; Song, W. B. (2018 a) Diversity of the cyrtophorid genus Chlamydodon (Protista, Ciliophora): its systematics and geographic distribution, with taxonomic descriptions of three species. Systematics and Biodiversity, 16, 497 - 511. https: // doi. org / 10.1080 / 14772000.2018.1456493" type="journal article" year="2018">
Qu
<emphasis id="B4D2EAFD9B77FFC82C1E1A95B855FE0B" box="[796,853,405,431]" italics="true" pageId="4" pageNumber="210">et al.</emphasis>
, 2018a
</bibRefCitation>
), each with a 95.5% sequence similarity. In phylogenetic trees (
<figureCitation id="1E9D2A6A9B77FFC82E701ABABAB7FE70" box="[370,439,441,468]" captionStart="FIGURE 6" captionStartId="8.[151,250,1854,1879]" captionTargetBox="[151,1436,651,1793]" captionTargetId="figure@8.[151,1436,651,1793]" captionTargetPageId="8" captionText="FIGURE 6. Phylogenetic tree of Maximum-likelihood (ML) based on small SSU rRNA gene sequences with Bayesian inference (BI) tapped on it. The four new sequences in the present paper are highlighted in bold. Numbers at nodes show ML bootstrap values and the BI posterior probabilities (ML/BI). Values with full support (ML/BI, 100/1.00) are marked with solid circle. The scale bar corresponds to 5 substitutions per 100 nucleotide positions. * Guangdong population of Chlamydodon bourlandi, ** misidentification, should be Chlamydodon bourlandi." httpUri="https://zenodo.org/record/3384542/files/figure.png" pageId="4" pageNumber="210">Fig. 6</figureCitation>
),
<taxonomicName id="41A64D6C9B77FFC82ECE1ABAB969FE77" authority="Wang &amp; Qu &amp; Hu, 2019" authorityName="Wang &amp; Qu &amp; Hu" authorityYear="2019" box="[460,617,441,467]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="4" pageNumber="210" phylum="Ciliophora" rank="species" species="pararoseus" status="sp. nov.">
<emphasis id="B4D2EAFD9B77FFC82ECE1ABAB969FE77" box="[460,617,441,467]" italics="true" pageId="4" pageNumber="210">C. pararoseus</emphasis>
</taxonomicName>
<emphasis id="B4D2EAFD9B77FFC82D721ABAB9AFFE77" bold="true" box="[624,687,441,467]" pageId="4" pageNumber="210">
<taxonomicNameLabel id="AFE157869B77FFC82D721ABAB9AFFE77" box="[624,687,441,467]" pageId="4" pageNumber="210" rank="species">sp. n.</taxonomicNameLabel>
</emphasis>
clusters with the clade comprising three
<taxonomicName id="41A64D6C9B77FFC82B7A1ABABE15FE77" authorityName="Ehrenberg" authorityYear="1835" box="[1144,1301,441,467]" class="Cyrtophoria" family="Chlamydodontidae" genus="Chlamydodon" kingdom="Protozoa" order="Chlamydodontida" pageId="4" pageNumber="210" phylum="Ciliophora" rank="genus">
<emphasis id="B4D2EAFD9B77FFC82B7A1ABABE15FE77" box="[1144,1301,441,467]" italics="true" pageId="4" pageNumber="210">Chlamydodon</emphasis>
</taxonomicName>
species and
<taxonomicName id="41A64D6C9B77FFC82F951ADEBAC4FE53" box="[151,452,477,503]" class="Cyrtophoria" family="Chlamydodontidae" genus="Paracyrtophoron" higherTaxonomySource="GBIF" kingdom="Chromista" order="Chlamydodontida" pageId="4" pageNumber="210" phylum="Ciliophora" rank="species" species="tropicum">
<emphasis id="B4D2EAFD9B77FFC82F951ADEBAC4FE53" box="[151,452,477,503]" italics="true" pageId="4" pageNumber="210">Paracyrtophoron tropicum</emphasis>
</taxonomicName>
, with medium to full support values (
<collectionCode id="E0B7AE2A9B77FFC82C6B1ADEB896FE53" box="[873,918,477,503]" country="France" name="Musee de Lectoure" pageId="4" pageNumber="210">ML</collectionCode>
/BI, 88%/1.00).
</paragraph>
</subSubSection>
</treatment>
</document>