318 lines
47 KiB
XML
318 lines
47 KiB
XML
<document id="D8DC0FE1537B925CAF0B386F560D3F62" ID-DOI="10.12651/JSR.2019.8.1.128" ID-ISSN="2713-8615" ID-Zenodo-Dep="8120462" IM.bibliography_approvedBy="felipe" IM.illustrations_approvedBy="felipe" IM.materialsCitations_approvedBy="felipe" IM.metadata_approvedBy="felipe" IM.tables_requiresApprovalFor="GgImagineBatch" IM.taxonomicNames_approvedBy="felipe" IM.treatments_approvedBy="felipe" checkinTime="1688645474366" checkinUser="felipe" docAuthor="Kim, Eun-Hee & Lee, Won Je" docDate="2019" docId="03965A7AFFE7691FF746F90BFD21F9C1" docLanguage="en" docName="JSpeciesRes.8.1.128-135.pdf" docOrigin="Journal of Species Research 8 (1)" docStyle="DocumentStyle:4D398BFF45FF8CA9E08F546BB06AE372.1:JSpeciesRes.2012-.journal_article" docStyleId="4D398BFF45FF8CA9E08F546BB06AE372" docStyleName="JSpeciesRes.2012-.journal_article" docStyleVersion="1" docTitle="Euplotes encysticus Yonezawa 1985" docType="treatment" docVersion="2" lastPageNumber="132" masterDocId="FFAF2202FFE5691BF46FFFECFF89FFAD" masterDocTitle="Redescriptions of Euplotes encysticus and E. rariseta (Protist: Ciliophora: Euplotida)" masterLastPageNumber="135" masterPageNumber="128" pageNumber="130" updateTime="1688645799251" updateUser="ExternalLinkService" zenodo-license-document="CC-BY-4.0">
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<mods:title id="5CCB8B91DDAEE04C681634445EC4DBE7">Redescriptions of Euplotes encysticus and E. rariseta (Protist: Ciliophora: Euplotida)</mods:title>
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<mods:name id="E23B9E0B1673E0B664905336DFF42EDC" type="personal">
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<mods:roleTerm id="A3F9C81ED0BD80E8C51E068C48CB4955">Author</mods:roleTerm>
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<mods:namePart id="60FFBA58C35CF0E9D30D60577FBFD3A2">Kim, Eun-Hee</mods:namePart>
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<mods:affiliation id="2B754D9A781E8BA5854D732B4A23834E">Department of Environment and Energy Engineering, Kyungnam University, Changwon 51767, Republic of Korea & W & S Ecosystem Assessment Institute, Masanhappo-gu, Changwon 51745, Republic of Korea</mods:affiliation>
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<mods:roleTerm id="87FED469E536D52F1B2AAE85826B4763">Author</mods:roleTerm>
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<mods:namePart id="67F92F7835FE77A08F7096645F19CD7C">Lee, Won Je</mods:namePart>
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<mods:affiliation id="2C6457BD0A0C1B62D948BAB2F7069512">Department of Environment and Energy Engineering, Kyungnam University, Changwon 51767, Republic of Korea</mods:affiliation>
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<mods:nameIdentifier id="842DC24BF821982FE39078BA3BA1E258" type="email">wonje@kyungnam.ac.kr</mods:nameIdentifier>
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<mods:title id="27413C598571286885F7DA079DD94024">Journal of Species Research</mods:title>
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<mods:date id="F1E361D52268CC5A5E21B39C4A05293C">2019</mods:date>
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<mods:number id="4855542C3618FFA66A0A425BEBE4CFC0">8</mods:number>
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<mods:detail id="026B7AFF8B441E4866F364C63D6EC94D" type="issue">
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<mods:number id="8F9D4FC190CEFE57B9958959FA6F1A62">1</mods:number>
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<mods:start id="235F90574181092257355A36A987548E">128</mods:start>
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<mods:identifier id="7968F591B9B99A92044916EED74AE37B" type="DOI">10.12651/JSR.2019.8.1.128</mods:identifier>
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<treatment id="03965A7AFFE7691FF746F90BFD21F9C1" LSID="urn:lsid:plazi:treatment:03965A7AFFE7691FF746F90BFD21F9C1" httpUri="http://treatment.plazi.org/id/03965A7AFFE7691FF746F90BFD21F9C1" lastPageId="4" lastPageNumber="132" pageId="2" pageNumber="130">
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<subSubSection id="C325B8E7FFE76919F746F90BFA08F952" box="[809,1409,1766,1791]" pageId="2" pageNumber="130" type="nomenclature">
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<paragraph id="8B80EB6CFFE76919F746F90BFA08F952" blockId="2.[809,1409,1766,1791]" box="[809,1409,1766,1791]" pageId="2" pageNumber="130">
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<heading id="D0C85C00FFE76919F746F90BFA08F952" bold="true" box="[809,1409,1766,1791]" centered="true" fontSize="36" level="2" pageId="2" pageNumber="130" reason="3">
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<emphasis id="B94B377EFFE76919F746F90BFA08F952" bold="true" box="[809,1409,1766,1791]" pageId="2" pageNumber="130">
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1.
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<taxonomicName id="4C3F90EFFFE76919F72CF90AFB45F952" ID-CoL="3CW9C" ID-ENA="440145" authority="Yonezawa, 1985" authorityName="Yonezawa" authorityYear="1985" box="[835,1228,1766,1791]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="2" pageNumber="130" phylum="Ciliophora" rank="species" species="encysticus">
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<emphasis id="B94B377EFFE76919F72CF90AFB9CF952" bold="true" box="[835,1045,1766,1791]" italics="true" pageId="2" pageNumber="130">Euplotes encysticus</emphasis>
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<bibRefCitation id="EFAE969DFFE76919F074F90BFB45F952" author="Yonezawa, F." box="[1051,1228,1767,1791]" pageId="2" pageNumber="130" pagination="35 - 45" refId="ref6162" refString="Yonezawa, F. 1985. New hypotrichous ciliate Euplotes encysticus sp. nov. Journal of Science of Hiroshima University 32: 35 - 45." type="journal article" year="1985">Yonezawa, 1985</bibRefCitation>
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</taxonomicName>
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(
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<figureCitation id="1304F7E9FFE76919F0B8F90BFA96F952" box="[1239,1311,1767,1791]" captionStart="Fig" captionStartId="1.[174,207,1241,1263]" captionTargetBox="[203,1414,261,1208]" captionTargetId="figure-309@1.[153,1438,181,1468]" captionTargetPageId="1" captionText="Fig. 1. Drawings of Euplotes encysticus (a-d) and E. rariseta (e-h). (a) E. encysticus and (e) E. rariseta in vivo, E. encysticus (b-d) and E. rariseta (f-h) after protargol impregnation. (b) and (f) ventral views, (c) and (g) dorsal views, (d) and (h) different shapes of macronucleus. AZM, adoral zone of membranelles; PM, paroral membrane; FVC, fronto-ventral cirri; TC, transverse cirri; CC, caudal cirri; MC, marginal cirrus; DK, dorsal kineties; Ma, macronucleus; Mi, micronucleus.Scale bars in (b) for (a-c) and in (d) represents 20μm, in (f) for (e-g) and in (h) represent 10 μm." figureDoi="http://doi.org/10.5281/zenodo.8120464" httpUri="https://zenodo.org/record/8120464/files/figure.png" pageId="2" pageNumber="130">Figs. 1</figureCitation>
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a-d, 2, 3)
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</emphasis>
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</heading>
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</paragraph>
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||
</subSubSection>
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<subSubSection id="C325B8E7FFE76919F746F8CBFA0FF86D" pageId="2" pageNumber="130" type="materials_examined">
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<paragraph id="8B80EB6CFFE76919F746F8CBFA0FF86D" blockId="2.[809,1414,1830,1985]" pageId="2" pageNumber="130">
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<emphasis id="B94B377EFFE76919F746F8CBFB87F892" bold="true" box="[809,1038,1831,1855]" pageId="2" pageNumber="130">Material examined.</emphasis>
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<materialsCitation id="3B57E131FFE76919F078F8C4FBE3F86D" collectingDate="2017-09-27" collectorName="Jong Soo Park. & Kyungnam University" country="South Korea" latitude="37.239445" location="Korean Culture Collection of Protists" longLatPrecision="19" longitude="131.8689" pageId="2" pageNumber="130" specimenCount="1">
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<collectingCountry id="F328ABFCFFE76919F078F8C4FBD7F892" box="[1047,1118,1832,1855]" name="South Korea" pageId="2" pageNumber="130">Korea</collectingCountry>
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: Dokdo (
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<geoCoordinate id="EE0B8DABFFE76919F0ADF8CBFACCF892" box="[1218,1349,1830,1856]" degrees="37" direction="north" minutes="14" orientation="latitude" pageId="2" pageNumber="130" precision="15" seconds="22" value="37.239445">
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37°14
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<emphasis id="B94B377EFFE76919F16BF8CAFA83F8ED" bold="true" box="[1284,1290,1830,1856]" italics="true" pageId="2" pageNumber="130">ʹ</emphasis>
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22
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<emphasis id="B94B377EFFE76919F14AF8CAFAA6F8ED" bold="true" box="[1317,1327,1830,1856]" italics="true" pageId="2" pageNumber="130">ʺ</emphasis>
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N
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</geoCoordinate>
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,
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<geoCoordinate id="EE0B8DABFFE76919F13CF8CBFC0BF8CD" degrees="131" direction="east" minutes="52" orientation="longitude" pageId="2" pageNumber="130" precision="15" seconds="08" value="131.8689">
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131° 52
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<emphasis id="B94B377EFFE76919F72BF8AAFCC3F8CD" bold="true" box="[836,842,1862,1888]" italics="true" pageId="2" pageNumber="130">ʹ</emphasis>
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08
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<emphasis id="B94B377EFFE76919F70AF8AAFCE6F8CD" bold="true" box="[869,879,1862,1888]" italics="true" pageId="2" pageNumber="130">ʺ</emphasis>
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E
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</geoCoordinate>
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),
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<date id="FF81CDACFFE76919F7F6F8A4FBFAF8CD" box="[921,1139,1863,1888]" pageId="2" pageNumber="130" value="2017-09-27">
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<collectingDate id="EFC53444FFE76919F7F6F8A4FBFAF8CD" box="[921,1139,1863,1888]" pageId="2" pageNumber="130" value="2017-09-27">27 September 2017</collectingDate>
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</date>
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, from a freshwater pond (Mulgol), collected by
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<collectorName id="26CA8EBAFFE76919F049F884FB44F82D" box="[1062,1229,1896,1920]" pageId="2" pageNumber="130">Jong Soo Park.</collectorName>
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<typeStatus id="548455CEFFE76919F0BBF884FA80F82D" box="[1236,1289,1896,1920]" pageId="2" pageNumber="130">Type</typeStatus>
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strain, live cells are kept with the
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<location id="8EE0BDB7FFE76919F05AF864FCF0F86D" LSID="urn:lsid:plazi:treatment:03965A7AFFE7691FF746F90BFD21F9C1:8EE0BDB7FFE76919F05AF864FCF0F86D" country="South Korea" latitude="37.239445" longLatPrecision="19" longitude="131.8689" name="Korean Culture Collection of Protists" pageId="2" pageNumber="130">Korean Culture Collection of Protists</location>
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,
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<collectorName id="26CA8EBAFFE76919F7EDF845FBE3F86D" box="[898,1130,1960,1985]" pageId="2" pageNumber="130">Kyungnam University</collectorName>
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</materialsCitation>
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,
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<collectingCountry id="F328ABFCFFE76919F01BF845FB3FF86D" box="[1140,1206,1961,1984]" name="South Korea" pageId="2" pageNumber="130">Korea</collectingCountry>
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, reference ‘KF403’.
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</paragraph>
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||
</subSubSection>
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||
<caption id="DF40BBE4FFE66918F4C1FC5FFB68FC49" ID-DOI="http://doi.org/10.5281/zenodo.8120468" ID-Zenodo-Dep="8120468" httpUri="https://zenodo.org/record/8120468/files/figure.png" pageId="3" pageNumber="131" startId="3.[174,207,947,969]" targetBox="[184,1435,257,915]" targetPageId="3" targetType="figure">
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<paragraph id="8B80EB6CFFE66918F4C1FC5FFB68FC49" blockId="3.[174,1443,947,996]" pageId="3" pageNumber="131">
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<emphasis id="B94B377EFFE66918F4C1FC5FFF65FC65" bold="true" box="[174,236,947,969]" pageId="3" pageNumber="131">Fig. 3.</emphasis>
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Micrographs of excystment of
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<emphasis id="B94B377EFFE66918F67DFC5FFD44FC64" box="[530,717,947,969]" italics="true" pageId="3" pageNumber="131">
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||
<taxonomicName id="4C3F90EFFFE66918F67DFC5FFD41FC64" authorityName="Yonezawa" authorityYear="1985" box="[530,712,947,969]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">Euplotes encysticus</taxonomicName>
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.
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</emphasis>
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(a) Cyst of cell 1, (b-j) time series lasting 6 minutes 52 seconds documenting excystment of cell 2, (i) empty cyst and trophozoit, (j) trophozoit. All images are DIC images. Scale bar in (j) = 25 μm.
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</paragraph>
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</caption>
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<subSubSection id="C325B8E7FFE66918F4C1FBC6FE70F8A5" pageId="3" pageNumber="131" type="description">
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<paragraph id="8B80EB6CFFE66918F4C1FBC6FE70F8A5" blockId="3.[174,779,1066,1994]" pageId="3" pageNumber="131">
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<emphasis id="B94B377EFFE66918F4C1FBC6FEC8FBEF" bold="true" box="[174,321,1066,1090]" pageId="3" pageNumber="131">Description.</emphasis>
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Cells are 63-79 × 41-61 μm in vivo and 44-56 × 28-38 μm in protargol, asymmetrically oval with posterior portion slightly wider than anterior, and dorso-ventrally flattened. The peristome is narrow and extremely long, extending approximately 72% of cell length and surrounded by 31-36 adoral membranelles. Adoral zone of membranelles (AZM) curved (
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<figureCitation id="1304F7E9FFE66918F6C4FB00FF42FA89" captionStart="Fig" captionStartId="1.[174,207,1241,1263]" captionTargetBox="[203,1414,261,1208]" captionTargetId="figure-309@1.[153,1438,181,1468]" captionTargetPageId="1" captionText="Fig. 1. Drawings of Euplotes encysticus (a-d) and E. rariseta (e-h). (a) E. encysticus and (e) E. rariseta in vivo, E. encysticus (b-d) and E. rariseta (f-h) after protargol impregnation. (b) and (f) ventral views, (c) and (g) dorsal views, (d) and (h) different shapes of macronucleus. AZM, adoral zone of membranelles; PM, paroral membrane; FVC, fronto-ventral cirri; TC, transverse cirri; CC, caudal cirri; MC, marginal cirrus; DK, dorsal kineties; Ma, macronucleus; Mi, micronucleus.Scale bars in (b) for (a-c) and in (d) represents 20μm, in (f) for (e-g) and in (h) represent 10 μm." figureDoi="http://doi.org/10.5281/zenodo.8120464" httpUri="https://zenodo.org/record/8120464/files/figure.png" pageId="3" pageNumber="131">Figs. 1a, 1b</figureCitation>
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,
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<figureCitation id="1304F7E9FFE66918F4B6FAE0FECEFA89" box="[217,327,1292,1316]" captionStart="Fig" captionStartId="2.[144,177,1439,1461]" captionTargetBox="[246,1304,257,1406]" captionTargetId="figure-124@2.[246,1304,257,1411]" captionTargetPageId="2" captionText="Fig. 2. Micrographs of Euplotes encysticus strain KF403 (a-c) in vivo and (d-f) after protargol impregnation. (a) Dorsal views showing contractile vacuole (CV) and adoral membranelles, (b) dorsal ridges (arrowheads), (c) ventral side showing FVC (fronto-ventral cirri) and TC (transvers cirri), (d) ventral view showing AZM (adoral zone of membranelles), PM (paroral membrane), FVC, TC, CC (caudal cirri), MC (marginal cirri), Ma (macronucleus), Mi (micronucleus), (e) dorsal side showing DK (dorsal kineties), (f) note 3 CC. Scale bars in (c) for (a-c) and in (f) for (d-f) represent 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8120466" httpUri="https://zenodo.org/record/8120466/files/figure.png" pageId="3" pageNumber="131">2a, 2d, 2f</figureCitation>
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). Cytoplasm colourless, often filled with many light reflecting granules and few food vacuoles (
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<figureCitation id="1304F7E9FFE66918F4D9FAA1FE85FAC8" box="[182,268,1357,1381]" captionStart="Fig" captionStartId="2.[144,177,1439,1461]" captionTargetBox="[246,1304,257,1406]" captionTargetId="figure-124@2.[246,1304,257,1411]" captionTargetPageId="2" captionText="Fig. 2. Micrographs of Euplotes encysticus strain KF403 (a-c) in vivo and (d-f) after protargol impregnation. (a) Dorsal views showing contractile vacuole (CV) and adoral membranelles, (b) dorsal ridges (arrowheads), (c) ventral side showing FVC (fronto-ventral cirri) and TC (transvers cirri), (d) ventral view showing AZM (adoral zone of membranelles), PM (paroral membrane), FVC, TC, CC (caudal cirri), MC (marginal cirri), Ma (macronucleus), Mi (micronucleus), (e) dorsal side showing DK (dorsal kineties), (f) note 3 CC. Scale bars in (c) for (a-c) and in (f) for (d-f) represent 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8120466" httpUri="https://zenodo.org/record/8120466/files/figure.png" pageId="3" pageNumber="131">Fig. 2a</figureCitation>
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). Single contractile vacuole located to right of transverse cirri (TC) (
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<figureCitation id="1304F7E9FFE66918F5ABFA81FD90FA28" box="[452,537,1389,1413]" captionStart="Fig" captionStartId="2.[144,177,1439,1461]" captionTargetBox="[246,1304,257,1406]" captionTargetId="figure-124@2.[246,1304,257,1411]" captionTargetPageId="2" captionText="Fig. 2. Micrographs of Euplotes encysticus strain KF403 (a-c) in vivo and (d-f) after protargol impregnation. (a) Dorsal views showing contractile vacuole (CV) and adoral membranelles, (b) dorsal ridges (arrowheads), (c) ventral side showing FVC (fronto-ventral cirri) and TC (transvers cirri), (d) ventral view showing AZM (adoral zone of membranelles), PM (paroral membrane), FVC, TC, CC (caudal cirri), MC (marginal cirri), Ma (macronucleus), Mi (micronucleus), (e) dorsal side showing DK (dorsal kineties), (f) note 3 CC. Scale bars in (c) for (a-c) and in (f) for (d-f) represent 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8120466" httpUri="https://zenodo.org/record/8120466/files/figure.png" pageId="3" pageNumber="131">Fig. 2a</figureCitation>
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). Seven dorso-lateral ridges (
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<figureCitation id="1304F7E9FFE66918F491FA61FED3FA08" box="[254,346,1421,1445]" captionStart="Fig" captionStartId="2.[144,177,1439,1461]" captionTargetBox="[246,1304,257,1406]" captionTargetId="figure-124@2.[246,1304,257,1411]" captionTargetPageId="2" captionText="Fig. 2. Micrographs of Euplotes encysticus strain KF403 (a-c) in vivo and (d-f) after protargol impregnation. (a) Dorsal views showing contractile vacuole (CV) and adoral membranelles, (b) dorsal ridges (arrowheads), (c) ventral side showing FVC (fronto-ventral cirri) and TC (transvers cirri), (d) ventral view showing AZM (adoral zone of membranelles), PM (paroral membrane), FVC, TC, CC (caudal cirri), MC (marginal cirri), Ma (macronucleus), Mi (micronucleus), (e) dorsal side showing DK (dorsal kineties), (f) note 3 CC. Scale bars in (c) for (a-c) and in (f) for (d-f) represent 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8120466" httpUri="https://zenodo.org/record/8120466/files/figure.png" pageId="3" pageNumber="131">Figs. 2b</figureCitation>
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, arrowheads; 2e). Dorsal kineties (DK) inserted in concaves down the ridges (
|
||
<figureCitation id="1304F7E9FFE66918F600FA41FD46FA6B" box="[623,719,1453,1478]" captionStart="Fig" captionStartId="1.[174,207,1241,1263]" captionTargetBox="[203,1414,261,1208]" captionTargetId="figure-309@1.[153,1438,181,1468]" captionTargetPageId="1" captionText="Fig. 1. Drawings of Euplotes encysticus (a-d) and E. rariseta (e-h). (a) E. encysticus and (e) E. rariseta in vivo, E. encysticus (b-d) and E. rariseta (f-h) after protargol impregnation. (b) and (f) ventral views, (c) and (g) dorsal views, (d) and (h) different shapes of macronucleus. AZM, adoral zone of membranelles; PM, paroral membrane; FVC, fronto-ventral cirri; TC, transverse cirri; CC, caudal cirri; MC, marginal cirrus; DK, dorsal kineties; Ma, macronucleus; Mi, micronucleus.Scale bars in (b) for (a-c) and in (d) represents 20μm, in (f) for (e-g) and in (h) represent 10 μm." figureDoi="http://doi.org/10.5281/zenodo.8120464" httpUri="https://zenodo.org/record/8120464/files/figure.png" pageId="3" pageNumber="131">Figs. 1c</figureCitation>
|
||
,
|
||
<figureCitation id="1304F7E9FFE66918F6B0FA42FD74FA6B" box="[735,765,1454,1478]" captionStart="Fig" captionStartId="2.[144,177,1439,1461]" captionTargetBox="[246,1304,257,1406]" captionTargetId="figure-124@2.[246,1304,257,1411]" captionTargetPageId="2" captionText="Fig. 2. Micrographs of Euplotes encysticus strain KF403 (a-c) in vivo and (d-f) after protargol impregnation. (a) Dorsal views showing contractile vacuole (CV) and adoral membranelles, (b) dorsal ridges (arrowheads), (c) ventral side showing FVC (fronto-ventral cirri) and TC (transvers cirri), (d) ventral view showing AZM (adoral zone of membranelles), PM (paroral membrane), FVC, TC, CC (caudal cirri), MC (marginal cirri), Ma (macronucleus), Mi (micronucleus), (e) dorsal side showing DK (dorsal kineties), (f) note 3 CC. Scale bars in (c) for (a-c) and in (f) for (d-f) represent 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8120466" httpUri="https://zenodo.org/record/8120466/files/figure.png" pageId="3" pageNumber="131">2e</figureCitation>
|
||
). The kinetosome number of the mid-dorsal kinety is 19- 22. Cirri usually strong; 9 fronto-ventral, 5 transverse, 2-3 (mostly 2) caudal and 2 left marginal cirri. Paroral membrane (PM) about 10 μm long. Macronucleus (Ma) usually C-shaped, with the anterior and posterior ends sometimes curving (
|
||
<figureCitation id="1304F7E9FFE66918F5EAF983FE57F92A" box="[389,478,1647,1671]" captionStart="Fig" captionStartId="1.[174,207,1241,1263]" captionTargetBox="[203,1414,261,1208]" captionTargetId="figure-309@1.[153,1438,181,1468]" captionTargetPageId="1" captionText="Fig. 1. Drawings of Euplotes encysticus (a-d) and E. rariseta (e-h). (a) E. encysticus and (e) E. rariseta in vivo, E. encysticus (b-d) and E. rariseta (f-h) after protargol impregnation. (b) and (f) ventral views, (c) and (g) dorsal views, (d) and (h) different shapes of macronucleus. AZM, adoral zone of membranelles; PM, paroral membrane; FVC, fronto-ventral cirri; TC, transverse cirri; CC, caudal cirri; MC, marginal cirrus; DK, dorsal kineties; Ma, macronucleus; Mi, micronucleus.Scale bars in (b) for (a-c) and in (d) represents 20μm, in (f) for (e-g) and in (h) represent 10 μm." figureDoi="http://doi.org/10.5281/zenodo.8120464" httpUri="https://zenodo.org/record/8120464/files/figure.png" pageId="3" pageNumber="131">Figs. 1d</figureCitation>
|
||
,
|
||
<figureCitation id="1304F7E9FFE66918F583F983FDB9F92A" box="[492,560,1647,1671]" captionStart="Fig" captionStartId="2.[144,177,1439,1461]" captionTargetBox="[246,1304,257,1406]" captionTargetId="figure-124@2.[246,1304,257,1411]" captionTargetPageId="2" captionText="Fig. 2. Micrographs of Euplotes encysticus strain KF403 (a-c) in vivo and (d-f) after protargol impregnation. (a) Dorsal views showing contractile vacuole (CV) and adoral membranelles, (b) dorsal ridges (arrowheads), (c) ventral side showing FVC (fronto-ventral cirri) and TC (transvers cirri), (d) ventral view showing AZM (adoral zone of membranelles), PM (paroral membrane), FVC, TC, CC (caudal cirri), MC (marginal cirri), Ma (macronucleus), Mi (micronucleus), (e) dorsal side showing DK (dorsal kineties), (f) note 3 CC. Scale bars in (c) for (a-c) and in (f) for (d-f) represent 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8120466" httpUri="https://zenodo.org/record/8120466/files/figure.png" pageId="3" pageNumber="131">2d, 2f</figureCitation>
|
||
). Micronucleus (Mi) small, nearly spherical and located in the upper right half of the macronucleus (
|
||
<figureCitation id="1304F7E9FFE66918F5ACF95CFDCAF965" box="[451,579,1712,1736]" captionStart="Fig" captionStartId="1.[174,207,1241,1263]" captionTargetBox="[203,1414,261,1208]" captionTargetId="figure-309@1.[153,1438,181,1468]" captionTargetPageId="1" captionText="Fig. 1. Drawings of Euplotes encysticus (a-d) and E. rariseta (e-h). (a) E. encysticus and (e) E. rariseta in vivo, E. encysticus (b-d) and E. rariseta (f-h) after protargol impregnation. (b) and (f) ventral views, (c) and (g) dorsal views, (d) and (h) different shapes of macronucleus. AZM, adoral zone of membranelles; PM, paroral membrane; FVC, fronto-ventral cirri; TC, transverse cirri; CC, caudal cirri; MC, marginal cirrus; DK, dorsal kineties; Ma, macronucleus; Mi, micronucleus.Scale bars in (b) for (a-c) and in (d) represents 20μm, in (f) for (e-g) and in (h) represent 10 μm." figureDoi="http://doi.org/10.5281/zenodo.8120464" httpUri="https://zenodo.org/record/8120464/files/figure.png" pageId="3" pageNumber="131">Figs. 1b, 1d</figureCitation>
|
||
,
|
||
<figureCitation id="1304F7E9FFE66918F63EF95CFDE7F965" box="[593,622,1712,1736]" captionStart="Fig" captionStartId="2.[144,177,1439,1461]" captionTargetBox="[246,1304,257,1406]" captionTargetId="figure-124@2.[246,1304,257,1411]" captionTargetPageId="2" captionText="Fig. 2. Micrographs of Euplotes encysticus strain KF403 (a-c) in vivo and (d-f) after protargol impregnation. (a) Dorsal views showing contractile vacuole (CV) and adoral membranelles, (b) dorsal ridges (arrowheads), (c) ventral side showing FVC (fronto-ventral cirri) and TC (transvers cirri), (d) ventral view showing AZM (adoral zone of membranelles), PM (paroral membrane), FVC, TC, CC (caudal cirri), MC (marginal cirri), Ma (macronucleus), Mi (micronucleus), (e) dorsal side showing DK (dorsal kineties), (f) note 3 CC. Scale bars in (c) for (a-c) and in (f) for (d-f) represent 20 μm." figureDoi="http://doi.org/10.5281/zenodo.8120466" httpUri="https://zenodo.org/record/8120466/files/figure.png" pageId="3" pageNumber="131">2d</figureCitation>
|
||
). The cells are capable of encystment and excystment (
|
||
<figureCitation id="1304F7E9FFE66918F63BF93CFD1FF945" box="[596,662,1744,1768]" captionStart="Fig" captionStartId="3.[174,207,947,969]" captionTargetBox="[184,1435,257,915]" captionTargetId="figure-520@3.[184,1435,257,915]" captionTargetPageId="3" captionText="Fig. 3. Micrographs of excystment of Euplotes encysticus. (a) Cyst of cell 1, (b-j) time series lasting 6 minutes 52 seconds documenting excystment of cell 2, (i) empty cyst and trophozoit, (j) trophozoit.All images are DIC images. Scale bar in(j)=25μm." figureDoi="http://doi.org/10.5281/zenodo.8120468" httpUri="https://zenodo.org/record/8120468/files/figure.png" pageId="3" pageNumber="131">Fig. 3</figureCitation>
|
||
). Move by crawling on crumb or substrate.
|
||
</paragraph>
|
||
</subSubSection>
|
||
<subSubSection id="C325B8E7FFE6691FF4C1F8FDFC7BFB69" lastPageId="4" lastPageNumber="132" pageId="3" pageNumber="131" type="discussion">
|
||
<paragraph id="8B80EB6CFFE66918F4C1F8FDFAFDF8A5" blockId="3.[174,779,1066,1994]" lastBlockId="3.[839,1444,1066,1994]" pageId="3" pageNumber="131">
|
||
<emphasis id="B94B377EFFE66918F4C1F8FDFE97F884" bold="true" box="[174,286,1809,1833]" pageId="3" pageNumber="131">Remarks.</emphasis>
|
||
<taxonomicName id="4C3F90EFFFE66918F548F8FCFD89F885" authorityName="Yonezawa" authorityYear="1985" box="[295,512,1808,1832]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F548F8FCFD89F885" box="[295,512,1808,1832]" italics="true" pageId="3" pageNumber="131">Euplotes encysticus</emphasis>
|
||
</taxonomicName>
|
||
was originally reported by
|
||
<bibRefCitation id="EFAE969DFFE66918F4A0F8DDFE0FF8E5" author="Yonezawa, F." box="[207,390,1840,1864]" pageId="3" pageNumber="131" pagination="35 - 45" refId="ref6162" refString="Yonezawa, F. 1985. New hypotrichous ciliate Euplotes encysticus sp. nov. Journal of Science of Hiroshima University 32: 35 - 45." type="journal article" year="1985">Yonezawa (1985)</bibRefCitation>
|
||
as a freshwater form. We performed the inoculation of our strain KF403 into well plates containing seawater (15 and 30 psu) with barley grains. The strain grew well in the seawater, thus this species is adaptable to both seawater and freshwater. Our observations are in accord with those of
|
||
<bibRefCitation id="EFAE969DFFE66918F081FBC7FA2DFBEF" author="Yonezawa, F." box="[1262,1444,1066,1090]" pageId="3" pageNumber="131" pagination="35 - 45" refId="ref6162" refString="Yonezawa, F. 1985. New hypotrichous ciliate Euplotes encysticus sp. nov. Journal of Science of Hiroshima University 32: 35 - 45." type="journal article" year="1985">Yonezawa (1985)</bibRefCitation>
|
||
and
|
||
<bibRefCitation id="EFAE969DFFE66918F71AFBA7FBA0FBCE" author="Fan, X. & J. Huang & X. Lin & J. Li & K. A. S. Al-Rasheid & X. Hu" box="[885,1065,1098,1123]" pageId="3" pageNumber="131" pagination="1411 - 1416" refId="ref4618" refString="Fan, X., J. Huang, X. Lin, J. Li, K. A. S. Al-Rasheid and X. Hu. 2010. Morphological and molecular characterization of Euplotes encysticus (Protozoa: Ciliophora: Euplotida). Journal of the Marine Biological Association of the United Kingdom 90: 1411 - 1416." type="journal article" year="2010">
|
||
Fan
|
||
<emphasis id="B94B377EFFE66918F7CAFBA7FC52FBCF" box="[933,987,1098,1122]" italics="true" pageId="3" pageNumber="131">et al.</emphasis>
|
||
(2010)
|
||
</bibRefCitation>
|
||
.
|
||
<taxonomicName id="4C3F90EFFFE66918F05BFBA6FA8AFBCF" authorityName="Yonezawa" authorityYear="1985" box="[1076,1283,1098,1122]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F05BFBA6FA8AFBCF" box="[1076,1283,1098,1122]" italics="true" pageId="3" pageNumber="131">Euplotes encysticus</emphasis>
|
||
</taxonomicName>
|
||
is very similar to
|
||
<taxonomicName id="4C3F90EFFFE66918F70BFB87FC7BFB2F" box="[868,1010,1131,1154]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="muscorum">
|
||
<emphasis id="B94B377EFFE66918F70BFB87FC7BFB2F" box="[868,1010,1131,1154]" italics="true" pageId="3" pageNumber="131">E. muscorum</emphasis>
|
||
</taxonomicName>
|
||
in general appearance, but according to several researchers (e.g.,
|
||
<bibRefCitation id="EFAE969DFFE66918F038FB60FA8AFB0E" author="Dragesco, J." box="[1111,1283,1163,1188]" pageId="3" pageNumber="131" refId="ref4573" refString="Dragesco, J. 1970. Cilies fibres du Cameroun. Yaound: Feder- al University of Cameroon Press." type="book" year="1970">Dragesco, 1970</bibRefCitation>
|
||
; Dragesco and Dragesco Kernéis, 1986;
|
||
<bibRefCitation id="EFAE969DFFE66918F039FB40FA90FB6E" author="Jo, J. O. & M. K. Shin" box="[1110,1305,1195,1219]" pageId="3" pageNumber="131" pagination="227 - 235" refId="ref4900" refString="Jo, J. O. and M. K. Shin. 2003. Redescription of newly record- ed ciliate, Euplotes muscorum (Ciliophora: Polyhymenophora: Hypotrichida) and comparison with related species from Korea. Korean J. Syst. Zool. 19: 227 - 235." type="journal article" year="2003">Jo and Shin, 2003</bibRefCitation>
|
||
) they can be distinguished from each other because (1) no cyst has been observed so far in
|
||
<taxonomicName id="4C3F90EFFFE66918F036FB00FB65FAAE" box="[1113,1260,1260,1283]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="muscorum">
|
||
<emphasis id="B94B377EFFE66918F036FB00FB65FAAE" box="[1113,1260,1260,1283]" italics="true" pageId="3" pageNumber="131">E. muscorum</emphasis>
|
||
</taxonomicName>
|
||
, (2) the obvious dorsal-lateral ridges and the granules surrounding the dorsal cirri have not observed in
|
||
<taxonomicName id="4C3F90EFFFE66918F0D4FAC1FAC4FAE9" box="[1211,1357,1325,1348]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="muscorum">
|
||
<emphasis id="B94B377EFFE66918F0D4FAC1FAC4FAE9" box="[1211,1357,1325,1348]" italics="true" pageId="3" pageNumber="131">E. muscorum</emphasis>
|
||
</taxonomicName>
|
||
, (3) the AZM of
|
||
<taxonomicName id="4C3F90EFFFE66918F7C0FAA1FBCCFAC9" authorityName="Yonezawa" authorityYear="1985" box="[943,1093,1357,1380]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F7C0FAA1FBCCFAC9" box="[943,1093,1357,1380]" italics="true" pageId="3" pageNumber="131">E. encysticus</emphasis>
|
||
</taxonomicName>
|
||
outspreads more broadly than that of
|
||
<taxonomicName id="4C3F90EFFFE66918F7F8FA82FBAFFA28" box="[919,1062,1390,1413]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="muscorum">
|
||
<emphasis id="B94B377EFFE66918F7F8FA82FBAFFA28" box="[919,1062,1390,1413]" italics="true" pageId="3" pageNumber="131">E. muscorum</emphasis>
|
||
</taxonomicName>
|
||
, and (4) the AZM covers 3/4 body length in
|
||
<taxonomicName id="4C3F90EFFFE66918F7DAFA62FBC1FA08" authorityName="Yonezawa" authorityYear="1985" box="[949,1096,1422,1445]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F7DAFA62FBC1FA08" box="[949,1096,1422,1445]" italics="true" pageId="3" pageNumber="131">E. encysticus</emphasis>
|
||
</taxonomicName>
|
||
, and 2/
|
||
<quantity id="4CC74689FFE66918F0CFFA61FB42FA08" box="[1184,1227,1421,1445]" metricMagnitude="-2" metricUnit="m" metricValue="7.62" pageId="3" pageNumber="131" unit="in" value="3.0">3 in</quantity>
|
||
<taxonomicName id="4C3F90EFFFE66918F0BAFA62FAE1FA08" box="[1237,1384,1422,1445]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="muscorum">
|
||
<emphasis id="B94B377EFFE66918F0BAFA62FAE1FA08" box="[1237,1384,1422,1445]" italics="true" pageId="3" pageNumber="131">E. muscorum</emphasis>
|
||
</taxonomicName>
|
||
. Our strain has all of these characters specific to
|
||
<taxonomicName id="4C3F90EFFFE66918F17BFA42FA14FA68" authorityName="Yonezawa" authorityYear="1985" box="[1300,1437,1454,1477]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F17BFA42FA14FA68" box="[1300,1437,1454,1477]" italics="true" pageId="3" pageNumber="131">E. encysticus</emphasis>
|
||
</taxonomicName>
|
||
. Additionally, these two species are clearly distinguished by the differences in their 18S rRNA gene sequences, of which
|
||
<taxonomicName id="4C3F90EFFFE66918F7E0F9E3FB90F98B" authorityName="Yonezawa" authorityYear="1985" box="[911,1049,1551,1574]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F7E0F9E3FB90F98B" box="[911,1049,1551,1574]" italics="true" pageId="3" pageNumber="131">E. encysticus</emphasis>
|
||
</taxonomicName>
|
||
differs from
|
||
<taxonomicName id="4C3F90EFFFE66918F0C6F9E3FABCF98B" box="[1193,1333,1551,1574]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="muscorum">
|
||
<emphasis id="B94B377EFFE66918F0C6F9E3FABCF98B" box="[1193,1333,1551,1574]" italics="true" pageId="3" pageNumber="131">E. muscorum</emphasis>
|
||
</taxonomicName>
|
||
by 2.04% (
|
||
<bibRefCitation id="EFAE969DFFE66918F721F9C3FB8FF9EA" author="Fan, X. & J. Huang & X. Lin & J. Li & K. A. S. Al-Rasheid & X. Hu" box="[846,1030,1582,1607]" pageId="3" pageNumber="131" pagination="1411 - 1416" refId="ref4618" refString="Fan, X., J. Huang, X. Lin, J. Li, K. A. S. Al-Rasheid and X. Hu. 2010. Morphological and molecular characterization of Euplotes encysticus (Protozoa: Ciliophora: Euplotida). Journal of the Marine Biological Association of the United Kingdom 90: 1411 - 1416." type="journal article" year="2010">
|
||
Fan
|
||
<emphasis id="B94B377EFFE66918F7EDF9C3FC35F9EB" box="[898,956,1582,1606]" italics="true" pageId="3" pageNumber="131">et al.</emphasis>
|
||
, 2010
|
||
</bibRefCitation>
|
||
).
|
||
<taxonomicName id="4C3F90EFFFE66918F074F9C2FB7DF9EB" authorityName="Yonezawa" authorityYear="1985" box="[1051,1268,1582,1606]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F074F9C2FB7DF9EB" box="[1051,1268,1582,1606]" italics="true" pageId="3" pageNumber="131">Euplotes encysticus</emphasis>
|
||
</taxonomicName>
|
||
is similar to
|
||
<emphasis id="B94B377EFFE66918F1E2F9C3FBA7F9CA" italics="true" pageId="3" pageNumber="131">
|
||
<taxonomicName id="4C3F90EFFFE66918F1E2F9C3FCF4F9CB" authorityName="Song & Wilbert" authorityYear="2009" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="petzi">E. petzi</taxonomicName>
|
||
,
|
||
<taxonomicName id="4C3F90EFFFE66918F7E4F9A3FBA7F9CA" authorityName="Dragesco" authorityYear="1966" box="[907,1070,1615,1639]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="roscoffensis">E. roscoffensis</taxonomicName>
|
||
</emphasis>
|
||
and
|
||
<taxonomicName id="4C3F90EFFFE66918F008F9A3FB5DF9CB" authorityName="Jiang, Zhang, Hu, Shao, Al-Rasheid & Song" authorityYear="2010" box="[1127,1236,1615,1638]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="sinicus">
|
||
<emphasis id="B94B377EFFE66918F008F9A3FB5DF9CB" box="[1127,1236,1615,1638]" italics="true" pageId="3" pageNumber="131">E. sinicus</emphasis>
|
||
</taxonomicName>
|
||
in cell length, but the numbers of AM, DK and dikinetids in mid-DK in
|
||
<taxonomicName id="4C3F90EFFFE66918F728F97CFC5DF90A" authorityName="Yonezawa" authorityYear="1985" box="[839,980,1680,1703]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F728F97CFC5DF90A" box="[839,980,1680,1703]" italics="true" pageId="3" pageNumber="131">E. encysticus</emphasis>
|
||
</taxonomicName>
|
||
are higher than those in
|
||
<emphasis id="B94B377EFFE66918F089F97CFC4EF96A" italics="true" pageId="3" pageNumber="131">
|
||
<taxonomicName id="4C3F90EFFFE66918F089F97CFAB2F90A" authorityName="Song & Wilbert" authorityYear="2009" box="[1254,1339,1680,1703]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="petzi">E. petzi</taxonomicName>
|
||
.
|
||
<taxonomicName id="4C3F90EFFFE66918F128F963FC4EF96A" authorityName="Dragesco" authorityYear="1966" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="roscoffensis">Euplotes roscoffensis</taxonomicName>
|
||
</emphasis>
|
||
and
|
||
<taxonomicName id="4C3F90EFFFE66918F06FF95CFBE5F96A" authorityName="Jiang, Zhang, Hu, Shao, Al-Rasheid & Song" authorityYear="2010" box="[1024,1132,1712,1735]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="sinicus">
|
||
<emphasis id="B94B377EFFE66918F06FF95CFBE5F96A" box="[1024,1132,1712,1735]" italics="true" pageId="3" pageNumber="131">E. sinicus</emphasis>
|
||
</taxonomicName>
|
||
have higher number of AM than that of
|
||
<taxonomicName id="4C3F90EFFFE66918F7A3F93CFBD1F94A" authorityName="Yonezawa" authorityYear="1985" box="[972,1112,1744,1767]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="encysticus">
|
||
<emphasis id="B94B377EFFE66918F7A3F93CFBD1F94A" box="[972,1112,1744,1767]" italics="true" pageId="3" pageNumber="131">E. encysticus</emphasis>
|
||
</taxonomicName>
|
||
(
|
||
<tableCitation id="C6BDDED7FFE66918F00AF93CFB48F945" box="[1125,1217,1744,1768]" captionStart="Table 1" captionStartId="5.[143,196,571,596]" captionText="Table 1. Morphometric data of Euplotes encysticus and E. rariseta. AZM, adoral zone of membranelles; CL, cell length; AM, adoral membranelles; FVC, frontoventral cirri; TC, transverse cirri; MC, marginal cirri; CC, caudal cirri; DK, dorsal kinety; DDK, dikinetids in mid-DK; PM, paroral membrane; n, number." pageId="3" pageNumber="131">Tables 1</tableCitation>
|
||
,
|
||
<tableCitation id="C6BDDED7FFE66918F0BFF93CFB69F945" box="[1232,1248,1744,1768]" captionStart="Table 2" captionStartId="5.[143,196,1291,1316]" captionText="Table 2. Comparison of morphologically similar Euplotes species. AM, adoral membranelles; FVC, frontoventral cirri; TC, transverse cirri; MC, marginal cirri; CC, caudal cirri; DK, dorsal kinety; DDK, dikinetids in mid-DK; n, number." pageId="3" pageNumber="131">2</tableCitation>
|
||
).
|
||
<taxonomicName id="4C3F90EFFFE66918F09AF93CFA2AF94A" authorityName="Jiang, Zhang, Hu, Shao, Al-Rasheid & Song" authorityYear="2010" box="[1269,1443,1744,1768]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="species" species="sinicus">
|
||
<emphasis id="B94B377EFFE66918F09AF93CFA2AF94A" box="[1269,1443,1744,1768]" italics="true" pageId="3" pageNumber="131">Euplotes sinicus</emphasis>
|
||
</taxonomicName>
|
||
has 11-16 dikinetids in mid-DK (vs 5-7) (
|
||
<tableCitation id="C6BDDED7FFE66918F09DF91CFAC2F8A5" box="[1266,1355,1776,1800]" captionStart="Table 1" captionStartId="5.[143,196,571,596]" captionText="Table 1. Morphometric data of Euplotes encysticus and E. rariseta. AZM, adoral zone of membranelles; CL, cell length; AM, adoral membranelles; FVC, frontoventral cirri; TC, transverse cirri; MC, marginal cirri; CC, caudal cirri; DK, dorsal kinety; DDK, dikinetids in mid-DK; PM, paroral membrane; n, number." pageId="3" pageNumber="131">Tables 1</tableCitation>
|
||
,
|
||
<tableCitation id="C6BDDED7FFE66918F137F91CFAE1F8A5" box="[1368,1384,1776,1800]" captionStart="Table 2" captionStartId="5.[143,196,1291,1316]" captionText="Table 2. Comparison of morphologically similar Euplotes species. AM, adoral membranelles; FVC, frontoventral cirri; TC, transverse cirri; MC, marginal cirri; CC, caudal cirri; DK, dorsal kinety; DDK, dikinetids in mid-DK; n, number." pageId="3" pageNumber="131">2</tableCitation>
|
||
).
|
||
</paragraph>
|
||
<paragraph id="8B80EB6CFFE66918F731F8FCFB85F804" blockId="3.[839,1444,1066,1994]" pageId="3" pageNumber="131">
|
||
NCBI BLASTN search result shows that the closest strains to our strain KF403 are
|
||
<taxonomicName id="4C3F90EFFFE66918F0FDF8DDFAD6F8E5" box="[1170,1375,1841,1864]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="3" pageNumber="131" phylum="Ciliophora" rank="subSpecies" species="encysticus" subSpecies="stains">
|
||
<emphasis id="B94B377EFFE66918F0FDF8DDFAD6F8E5" box="[1170,1375,1841,1864]" italics="true" pageId="3" pageNumber="131">E. encysticus stains</emphasis>
|
||
</taxonomicName>
|
||
[GenBank Accession Nos. FJ346569, EF535728, LN864512; Identity 99%]. The 18S rRNA sequence of our stain is 1,663 bp in length.
|
||
</paragraph>
|
||
<paragraph id="8B80EB6CFFE66918F728F85EFB2AF867" blockId="3.[839,1444,1066,1994]" box="[839,1187,1970,1994]" pageId="3" pageNumber="131">
|
||
<emphasis id="B94B377EFFE66918F728F85EFC2FF867" bold="true" box="[839,934,1970,1994]" pageId="3" pageNumber="131">Habitat.</emphasis>
|
||
Marine and Freshwater
|
||
</paragraph>
|
||
<paragraph id="8B80EB6CFFE1691FF48EFB73FC7BFB69" blockId="4.[225,633,1183,1220]" box="[225,1010,1183,1220]" lastBlockId="4.[997,1010,1183,1220]" pageId="4" pageNumber="132">
|
||
<emphasis id="B94B377EFFE1691FF48EFB73FC7BFB69" bold="true" box="[225,1010,1183,1220]" pageId="4" pageNumber="132">d e f</emphasis>
|
||
</paragraph>
|
||
</subSubSection>
|
||
<caption id="DF40BBE4FFE1691FF4FFFB07FEFCFAC1" ID-DOI="http://doi.org/10.5281/zenodo.8120470" ID-Zenodo-Dep="8120470" httpUri="https://zenodo.org/record/8120470/files/figure.png" pageId="4" pageNumber="132" startId="4.[144,177,1259,1281]" targetBox="[210,1347,257,1198]" targetPageId="4" targetType="figure">
|
||
<paragraph id="8B80EB6CFFE1691FF4FFFB07FEFCFAC1" blockId="4.[144,1414,1259,1388]" pageId="4" pageNumber="132">
|
||
<emphasis id="B94B377EFFE1691FF4FFFB07FF45FAAC" bold="true" box="[144,204,1259,1281]" pageId="4" pageNumber="132">Fig. 4.</emphasis>
|
||
Micrographs of
|
||
<taxonomicName id="4C3F90EFFFE1691FF509FB07FD8AFAAC" box="[358,515,1259,1281]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="132" phylum="Ciliophora" rank="species" species="rariseta">
|
||
<emphasis id="B94B377EFFE1691FF509FB07FD8AFAAC" box="[358,515,1259,1281]" italics="true" pageId="4" pageNumber="132">Euplotes rariseta</emphasis>
|
||
</taxonomicName>
|
||
strain KM401 (a-c) in vivo and strain KM444 (d-f) after protargol impregnation. (a) Dorsal ridges (arrowheads) of
|
||
<taxonomicName id="4C3F90EFFFE1691FF548FAEBFE03FAB1" box="[295,394,1287,1308]" class="Hypotrichea" family="Euplotidae" genus="Euplotes" kingdom="Chromista" order="Euplotida" pageId="4" pageNumber="132" phylum="Ciliophora" rank="species" species="rariseta">
|
||
<emphasis id="B94B377EFFE1691FF548FAEBFE03FAB1" box="[295,394,1287,1308]" italics="true" pageId="4" pageNumber="132">E. rariseta</emphasis>
|
||
</taxonomicName>
|
||
, (b) ventral view showing longitudinal ridges (arrowheads), (c) ventral view showing FVC and TC, (d) ventral view showing AZM (adoral zone of membranelles), FVC (fronto-ventral cirri), TC (transvers cirri), PM (paroral membrane), (e) dorsal view showing dorsal kineties, (f) ventral view showing ventral kineties and Ma (macronucleus). Scale bars in (c) for (a-c) represent 20 μm and in (f) for (d-f) represent 10 μm.
|
||
</paragraph>
|
||
</caption>
|
||
<subSubSection id="C325B8E7FFE1691FF4FFFA5EFD21F9C1" pageId="4" pageNumber="132" type="distribution">
|
||
<paragraph id="8B80EB6CFFE1691FF4FFFA5EFE3FF9A6" blockId="4.[144,748,1458,1644]" pageId="4" pageNumber="132">
|
||
<emphasis id="B94B377EFFE1691FF4FFFA5EFEA1FA67" bold="true" box="[144,296,1458,1482]" pageId="4" pageNumber="132">Distribution.</emphasis>
|
||
Freshwater habitats:
|
||
<collectingCountry id="F328ABFCFFE1691FF675FA5FFDD5FA67" box="[538,604,1459,1482]" name="South Korea" pageId="4" pageNumber="132">Korea</collectingCountry>
|
||
(Dokdo, this study),
|
||
<collectingCountry id="F328ABFCFFE1691FF48DFA3FFE96FA47" box="[226,287,1491,1514]" name="Japan" pageId="4" pageNumber="132">Japan</collectingCountry>
|
||
(
|
||
<bibRefCitation id="EFAE969DFFE1691FF543FA3FFE6BFA46" author="Yonezawa, F." box="[300,482,1491,1515]" pageId="4" pageNumber="132" pagination="35 - 45" refId="ref6162" refString="Yonezawa, F. 1985. New hypotrichous ciliate Euplotes encysticus sp. nov. Journal of Science of Hiroshima University 32: 35 - 45." type="journal article" year="1985">Yonezawa, 1985</bibRefCitation>
|
||
) Marine habitats:
|
||
<collectingCountry id="F328ABFCFFE1691FF6C3FA3FFD65FA46" box="[684,748,1491,1515]" name="China" pageId="4" pageNumber="132">China</collectingCountry>
|
||
(Daya Bay,
|
||
<bibRefCitation id="EFAE969DFFE1691FF564FA1FFE26F9A6" author="Fan, X. & J. Huang & X. Lin & J. Li & K. A. S. Al-Rasheid & X. Hu" box="[267,431,1523,1547]" pageId="4" pageNumber="132" pagination="1411 - 1416" refId="ref4618" refString="Fan, X., J. Huang, X. Lin, J. Li, K. A. S. Al-Rasheid and X. Hu. 2010. Morphological and molecular characterization of Euplotes encysticus (Protozoa: Ciliophora: Euplotida). Journal of the Marine Biological Association of the United Kingdom 90: 1411 - 1416." type="journal article" year="2010">
|
||
Fan
|
||
<emphasis id="B94B377EFFE1691FF556FA18FEFAF9A6" box="[313,371,1523,1547]" italics="true" pageId="4" pageNumber="132">et al.,</emphasis>
|
||
2010
|
||
</bibRefCitation>
|
||
)
|
||
</paragraph>
|
||
<paragraph id="8B80EB6CFFE1691FF4FFF9FFFE4EF9E1" blockId="4.[144,748,1458,1644]" pageId="4" pageNumber="132">
|
||
<emphasis id="B94B377EFFE1691FF4FFF9FFFE98F986" bold="true" box="[144,273,1555,1579]" pageId="4" pageNumber="132">Deposition.</emphasis>
|
||
National Institute of Biological Resources,
|
||
<collectingCountry id="F328ABFCFFE1691FF4FFF9D8FF59F9E6" box="[144,208,1588,1611]" name="South Korea" pageId="4" pageNumber="132">Korea</collectingCountry>
|
||
(NIBRPR0000109440)
|
||
</paragraph>
|
||
<paragraph id="8B80EB6CFFE1691FF4FFF9B8FD21F9C1" blockId="4.[144,748,1458,1644]" box="[144,680,1620,1644]" pageId="4" pageNumber="132">
|
||
<emphasis id="B94B377EFFE1691FF4FFF9B8FD96F9C1" bold="true" box="[144,543,1620,1644]" pageId="4" pageNumber="132">NCBI GenBank Accession Number</emphasis>
|
||
: MK026950
|
||
</paragraph>
|
||
</subSubSection>
|
||
</treatment>
|
||
</document> |