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<document ID-DOI="http://dx.doi.org/10.3897/mycokeys.70.52043" ID-GBIF-Dataset="1572d43f-352a-436b-a5c9-fe535dbda8b2" ID-PMC="PMC7387365" ID-Pensoft-Pub="1314-4049-70-19" ID-Pensoft-UUID="1D7B9142CED75FA3A9768E33977830C5" ID-PubMed="32742179" ModsDocID="1314-4049-70-19" checkinTime="1595392387876" checkinUser="pensoft" docAuthor="Samarakoon, Binu C., Phookamsak, Rungtiwa, Wanasinghe, Dhanushka N., Chomnunti, Putarak, Hyde, Kevin D., McKenzie, Eric H. C., Promputtha, Itthayakorn, Xu, Jian-Chu &amp; Li, Yun-Ju" docDate="2020" docId="888C2C142FA05BEE878F04509EB0C4E1" docLanguage="en" docName="MycoKeys 70: 19-37" docOrigin="MycoKeys 70" docSource="http://dx.doi.org/10.3897/mycokeys.70.52043" docTitle="Spegazzinia musae Samarakoon, Phookamsak, Wanas., Chomnunti &amp; K. D. Hyde 2020, sp. nov." docType="treatment" docVersion="4" id="1D7B9142CED75FA3A9768E33977830C5" lastPageNumber="19" masterDocId="1D7B9142CED75FA3A9768E33977830C5" masterDocTitle="Taxonomy and phylogenetic appraisal of Spegazzinia musae sp. nov. and S. deightonii (Didymosphaeriaceae, Pleosporales) on Musaceae from Thailand" masterLastPageNumber="37" masterPageNumber="19" pageNumber="19" updateTime="1668243314089" updateUser="ExternalLinkService">
<mods:mods xmlns:mods="http://www.loc.gov/mods/v3">
<mods:titleInfo>
<mods:title>Taxonomy and phylogenetic appraisal of Spegazzinia musae sp. nov. and S. deightonii (Didymosphaeriaceae, Pleosporales) on Musaceae from Thailand</mods:title>
</mods:titleInfo>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Samarakoon, Binu C.</mods:namePart>
<mods:affiliation>The State Phosphorus Resource Development and Utilization Engineering Technology Research Centre, Yunnan Phosphate Chemical Group Co. Ltd, Kunming 650201, China &amp; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Phookamsak, Rungtiwa</mods:namePart>
<mods:affiliation>Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China &amp; East and Central Asia Regional Office, World Agroforestry Centre (ICRAF), Kunming 650201, Yunnan, China &amp; Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe County, Yunnan, China &amp; Institute of Plant Health, Zhongkai University of Agriculture and Engineering, Guang Dong Province, China &amp; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand</mods:affiliation>
<mods:nameIdentifier type="orcid">https://orcid.org/0000-0002-6321-8416</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Wanasinghe, Dhanushka N.</mods:namePart>
<mods:affiliation>Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China &amp; East and Central Asia Regional Office, World Agroforestry Centre (ICRAF), Kunming 650201, Yunnan, China &amp; Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe County, Yunnan, China &amp; Institute of Plant Health, Zhongkai University of Agriculture and Engineering, Guang Dong Province, China</mods:affiliation>
<mods:nameIdentifier type="orcid">https://orcid.org/0000-0003-1759-3933</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Chomnunti, Putarak</mods:namePart>
<mods:affiliation>School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Hyde, Kevin D.</mods:namePart>
<mods:affiliation>School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand &amp; Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China &amp; Institute of Plant Health, Zhongkai University of Agriculture and Engineering, Guang Dong Province, China</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>McKenzie, Eric H. C.</mods:namePart>
<mods:affiliation>Landcare Research Manaaki Whenua, Private Bag 92170, Auckland, New Zealand</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Promputtha, Itthayakorn</mods:namePart>
<mods:affiliation>Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand &amp; Research Center in Bioresources for Agriculture, Industry and Medicine, Chiang Mai University, Chiang Mai 50200, Thailand</mods:affiliation>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Xu, Jian-Chu</mods:namePart>
<mods:affiliation>Key Laboratory for Plant Biodiversity and Biogeography of East Asia (KLPB), Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China &amp; East and Central Asia Regional Office, World Agroforestry Centre (ICRAF), Kunming 650201, Yunnan, China &amp; Honghe Center for Mountain Futures, Kunming Institute of Botany, Chinese Academy of Sciences, Honghe County, Yunnan, China</mods:affiliation>
<mods:nameIdentifier type="email">jxu@mail.kib.ac.cn</mods:nameIdentifier>
</mods:name>
<mods:name type="personal">
<mods:role>
<mods:roleTerm>Author</mods:roleTerm>
</mods:role>
<mods:namePart>Li, Yun-Ju</mods:namePart>
<mods:affiliation>The State Phosphorus Resource Development and Utilization Engineering Technology Research Centre, Yunnan Phosphate Chemical Group Co. Ltd, Kunming 650201, China</mods:affiliation>
<mods:nameIdentifier type="email">93905507@qq.com</mods:nameIdentifier>
</mods:name>
<mods:typeOfResource>text</mods:typeOfResource>
<mods:relatedItem type="host">
<mods:titleInfo>
<mods:title>MycoKeys</mods:title>
</mods:titleInfo>
<mods:part>
<mods:date>2020</mods:date>
<mods:detail type="volume">
<mods:number>70</mods:number>
</mods:detail>
<mods:extent unit="page">
<mods:start>19</mods:start>
<mods:end>37</mods:end>
</mods:extent>
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<mods:location>
<mods:url>http://dx.doi.org/10.3897/mycokeys.70.52043</mods:url>
</mods:location>
<mods:classification>journal article</mods:classification>
<mods:identifier type="DOI">http://dx.doi.org/10.3897/mycokeys.70.52043</mods:identifier>
<mods:identifier type="Pensoft-Pub">1314-4049-70-19</mods:identifier>
<mods:identifier type="Pensoft-UUID">1D7B9142CED75FA3A9768E33977830C5</mods:identifier>
</mods:mods>
<treatment ID-GBIF-Taxon="165857308" LSID="urn:lsid:plazi:treatment:888C2C142FA05BEE878F04509EB0C4E1" httpUri="http://treatment.plazi.org/id/888C2C142FA05BEE878F04509EB0C4E1" lastPageNumber="19" pageId="0" pageNumber="19">
<subSubSection pageId="0" pageNumber="19" type="nomenclature">
<paragraph pageId="0" pageNumber="19">
<taxonomicName LSID="888C2C14-2FA0-5BEE-878F-04509EB0C4E1" authority="Samarakoon, Phookamsak, Wanas., Chomnunti &amp; K. D. Hyde" authorityName="Samarakoon, Phookamsak, Wanas., Chomnunti &amp; K. D. Hyde" authorityYear="2020" class="Dothideomycetes" family="Didymosphaeriaceae" genus="Spegazzinia" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spegazzinia musae" order="Pleosporales" pageId="0" pageNumber="19" phylum="Ascomycota" rank="species" species="musae" status="sp. nov.">Spegazzinia musae Samarakoon, Phookamsak, Wanas., Chomnunti &amp; K.D. Hyde</taxonomicName>
<taxonomicNameLabel pageId="0" pageNumber="19">sp. nov.</taxonomicNameLabel>
<figureCitation captionStart="Figure 3" captionStartId="F3" captionText="Figure 3. Spegazzinia musae (MFLU 19 - 2907, holotype) a-c fungal colonies on host surface d mature conidia e conidiophore of α conidia with the mother cell f, g α conidia h-q β conidia r colony on PDA after 28 days. Scale bars: 200 μm (a-c), 20 μm (d-g, j), 10 μm (h, i, k-q)." figureDoi="10.3897/mycokeys.70.52043.figure3" httpUri="https://binary.pensoft.net/fig/434239" pageId="0" pageNumber="19">Figure 3</figureCitation>
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="19" type="etymology">
<paragraph pageId="0" pageNumber="19">Etymology.</paragraph>
<paragraph pageId="0" pageNumber="19">
The name reflects the host genus,
<taxonomicName class="Liliopsida" family="Musaceae" genus="Musa" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Musa" order="Zingiberales" pageId="0" pageNumber="19" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="19">Musa</emphasis>
</taxonomicName>
(
<taxonomicName family="Musaceae" lsidName="" pageId="0" pageNumber="19" rank="family">Musaceae</taxonomicName>
).
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="19" type="holotype">
<paragraph pageId="0" pageNumber="19">Holotype.</paragraph>
<paragraph pageId="0" pageNumber="19">MFLU 19-2907</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="19" type="description">
<paragraph pageId="0" pageNumber="19">Description.</paragraph>
<paragraph pageId="0" pageNumber="19">
<emphasis italics="true" pageId="0" pageNumber="19">Saprobic</emphasis>
on a dead leaf of
<taxonomicName class="Liliopsida" family="Musaceae" genus="Musa" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Musa" order="Zingiberales" pageId="0" pageNumber="19" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="19">Musa</emphasis>
</taxonomicName>
sp.
<emphasis bold="true" pageId="0" pageNumber="19">Sexual morph</emphasis>
Undetermined.
<emphasis bold="true" pageId="0" pageNumber="19">Asexual morph</emphasis>
Hyphomycetous.
<emphasis italics="true" pageId="0" pageNumber="19">Sporodochia</emphasis>
dark, dense, dry, powdery, velvety, 1-2 mm diameter.
<emphasis italics="true" pageId="0" pageNumber="19">Conidiophore mother cells</emphasis>
3.4-5.8
<normalizedToken originalValue="×">x</normalizedToken>
3.7-4.7
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis italics="true" pageId="0" pageNumber="19"></emphasis>
= 4.6
<normalizedToken originalValue="×">x</normalizedToken>
4.1
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 10) subhyaline or light brown, doliiform or subspherical.
<emphasis italics="true" pageId="0" pageNumber="19">Conidiophores</emphasis>
usually short to long bearing two types of conidia referred to here as
<normalizedToken originalValue="α">α</normalizedToken>
and
<normalizedToken originalValue="β">β</normalizedToken>
.
<emphasis italics="true" pageId="0" pageNumber="19">Conidiophores</emphasis>
of
<normalizedToken originalValue="α">α</normalizedToken>
<emphasis italics="true" pageId="0" pageNumber="19">conidia</emphasis>
up to 40-85
<normalizedToken originalValue="×">x</normalizedToken>
0.8-2.5
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis italics="true" pageId="0" pageNumber="19"></emphasis>
= 64
<normalizedToken originalValue="×">x</normalizedToken>
21.7
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 15), pale brown or dark golden brown, rough-walled, hyaline at bottom near the conidiophore mother cell, pale brown at middle, dark golden brown at top near conidial cells, erect or flexuous, narrow and long, generally unbranched, rarely branched.
<emphasis italics="true" pageId="0" pageNumber="19">Conidiophores</emphasis>
of
<normalizedToken originalValue="β">β</normalizedToken>
<emphasis italics="true" pageId="0" pageNumber="19">conidia</emphasis>
0.7-3.5
<normalizedToken originalValue="×">x</normalizedToken>
1.5-3
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis italics="true" pageId="0" pageNumber="19"></emphasis>
= 1.9
<normalizedToken originalValue="×">x</normalizedToken>
2.3
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 15) short, erect, unbranched, hyaline when immature, subhyaline or hyaline at maturity.
<emphasis italics="true" pageId="0" pageNumber="19">Conidiogenous cell development</emphasis>
basauxic, forming a single, terminal holoblastic conidium at the apex of conidiophore.
<emphasis italics="true" pageId="0" pageNumber="19">Conidial development</emphasis>
holoblastic.
<emphasis italics="true" pageId="0" pageNumber="19">Conidia</emphasis>
solitary, dry, two types:
<normalizedToken originalValue="α">α</normalizedToken>
<emphasis italics="true" pageId="0" pageNumber="19">conidia</emphasis>
stellate, 15-22.7
<normalizedToken originalValue="×">x</normalizedToken>
14.5-20.5
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis italics="true" pageId="0" pageNumber="19"></emphasis>
= 18.8
<normalizedToken originalValue="×">x</normalizedToken>
17.8
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 15), 4-6 celled, each cell globose to subglobose, deeply constricted at the septa, conspicuously spinulate, 4-6 spines, each 2-8
<normalizedToken originalValue="μm">μm</normalizedToken>
long arise from surface of each cell.
<normalizedToken originalValue="β">β</normalizedToken>
<emphasis italics="true" pageId="0" pageNumber="19">conidia</emphasis>
disc-shaped, initially hyaline, 4-celled, each cell slightly turbinate in shape, rough-walled, crossed septate, becoming brown to dark brown at maturity, each cell turbinate, crossed-septate, smooth-walled, light brown at the center near the septa, dark brown at periphery in constricted areas, 9.3-14.2
<normalizedToken originalValue="×">x</normalizedToken>
8.4-12.5
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis italics="true" pageId="0" pageNumber="19"></emphasis>
= 12.7
<normalizedToken originalValue="×">x</normalizedToken>
10.8
<normalizedToken originalValue="μm">μm</normalizedToken>
, n = 40), somewhat obovoid, deeply constricted at the septa, flat from side view, frequently with attached conidiogenous cells when splitting from the conidiophores.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="19" type="description">
<paragraph pageId="0" pageNumber="19">Culture characteristics.</paragraph>
<paragraph pageId="0" pageNumber="19">Conidia germinating on PDA within 12-15 h, germ tubes produced from one or several cells. Colonies growing on PDA, reaching a diameter of 46 mm after 14 d at 25 °C, greyish white, unevenly raised, surface rough, moderately dense, radially striated at center, margin crenulate; reverse white to greyish white.</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="19" type="materials_examined">
<paragraph pageId="0" pageNumber="19">Material examined.</paragraph>
<paragraph pageId="0" pageNumber="19">
Thailand, Nan Province, on a dead leaf of
<taxonomicName class="Liliopsida" family="Musaceae" genus="Musa" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Musa" order="Zingiberales" pageId="0" pageNumber="19" phylum="Tracheophyta" rank="genus">
<emphasis italics="true" pageId="0" pageNumber="19">Musa</emphasis>
</taxonomicName>
sp. (
<taxonomicName family="Musaceae" lsidName="" pageId="0" pageNumber="19" rank="family">Musaceae</taxonomicName>
), 12 September 2018, B.C. Samarakoon, BNS 069 (MFLU 19-2907,
<emphasis bold="true" pageId="0" pageNumber="19">holotype</emphasis>
), ex-type living culture MFLUCC 20-0001.
</paragraph>
</subSubSection>
<subSubSection pageId="0" pageNumber="19" type="notes">
<paragraph pageId="0" pageNumber="19">Notes.</paragraph>
<paragraph pageId="0" pageNumber="19">
Based on BLASTn search results of SSU, LSU, ITS and TEF1-α sequence data,
<taxonomicName authorityName="Samarakoon, Phookamsak, Wanas., Chomnunti &amp; K. D. Hyde" authorityYear="2020" class="Dothideomycetes" family="Microthyriaceae" genus="Spegazzinia" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spegazzinia musae" order="Microthyriales" pageId="0" pageNumber="19" phylum="Ascomycota" rank="species" species="musae">
<emphasis italics="true" pageId="0" pageNumber="19">Spegazzinia musae</emphasis>
</taxonomicName>
showed a high similarity (SSU = 98.24%, LSU = 98.92%, ITS = 96.91%, TEF1-α = 98.11%) to
<taxonomicName lsidName="S. neosundara" pageId="0" pageNumber="19" rank="species" species="neosundara">
<emphasis italics="true" pageId="0" pageNumber="19">S. neosundara</emphasis>
</taxonomicName>
(MFLUCC 15-0456). In the multigene phylogeny,
<taxonomicName lsidName="S. musae" pageId="0" pageNumber="19" rank="species" species="musae">
<emphasis italics="true" pageId="0" pageNumber="19">S. musae</emphasis>
</taxonomicName>
groups as a sister taxon to
<taxonomicName lsidName="S. deightonii" pageId="0" pageNumber="19" rank="species" species="deightonii">
<emphasis italics="true" pageId="0" pageNumber="19">S. deightonii</emphasis>
</taxonomicName>
with strong statistical support (77% ML, 0.99 BYPP) (Figure
<figureCitation captionStart="Figure 1" captionStartId="F1" captionText="Figure 1. Maximum likelihood tree revealed by RAxML from an analysis of SSU, LSU and ITS and TEF 1 - α sequence data of selected genera of family Didymosphaeriaceae, showing the phylogenetic position of Spegazzinia musae (MFLUCC 20 - 0001) and S. deightonii (MFLUCC 20 - 0002). ML bootstrap supports (≥ 60 %) and Bayesian posterior probabilities (≥ 0.95 BYPP) are given above in the branches, respectively. The tree was rooted with Pleospora herbarum and Stemphylium botryosum (Pleosporaceae). Strains generated in this study are indicated in red-bold. Ex-type species are indicated in bold. The scale bar represents the expected number of nucleotide substitutions per site. A best scoring RAxML tree is shown with a final ML optimization likelihood value of - 13516.66. The matrix had 795 distinct alignment patterns, with 33.60 % of undetermined characters or gaps. Estimated base frequencies were: A = 0.239862, C = 0.245185, G = 0.277025, T = 0.237927; substitution rates AC = 1.626982, AG = 2.468452, AT = 1.211822, CG = 1.092437, CT = 6.295657, GT = 1.000000; proportion of invariable sites I = 0.484119; gamma distribution shape parameter α = 0.445929." figureDoi="10.3897/mycokeys.70.52043.figure1" httpUri="https://binary.pensoft.net/fig/434237" pageId="0" pageNumber="19">1</figureCitation>
). Also, ITS sequence comparison revealed 3.75% base pair differences between
<taxonomicName lsidName="S. musae" pageId="0" pageNumber="19" rank="species" species="musae">
<emphasis italics="true" pageId="0" pageNumber="19">S. musae</emphasis>
</taxonomicName>
and
<taxonomicName lsidName="S. deightonii" pageId="0" pageNumber="19" rank="species" species="deightonii">
<emphasis italics="true" pageId="0" pageNumber="19">S. deightonii</emphasis>
</taxonomicName>
, which is in agreement with the species concept outlined by
<bibRefCitation DOI="https://doi.org/10.5943/mycosphere/7/11/4" author="Jeewon, R" journalOrPublisher="Mycosphere" pageId="0" pageNumber="19" pagination="1669 - 1677" refId="B29" refString="Jeewon, R, Hyde, KD, 2016. Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669 - 1677, DOI: https://doi.org/10.5943/mycosphere/7/11/4" title="Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities." url="https://doi.org/10.5943/mycosphere/7/11/4" volume="7" year="2016">Jeewon and Hyde (2016)</bibRefCitation>
. Besides,
<taxonomicName lsidName="S. musae" pageId="0" pageNumber="19" rank="species" species="musae">
<emphasis italics="true" pageId="0" pageNumber="19">S. musae</emphasis>
</taxonomicName>
has contrasting morphological features to
<taxonomicName lsidName="S. deightonii" pageId="0" pageNumber="19" rank="species" species="deightonii">
<emphasis italics="true" pageId="0" pageNumber="19">S. deightonii</emphasis>
</taxonomicName>
in both kinds of conidia. The disk-shaped conidia of
<taxonomicName lsidName="S. musae" pageId="0" pageNumber="19" rank="species" species="musae">
<emphasis italics="true" pageId="0" pageNumber="19">S. musae</emphasis>
</taxonomicName>
are 4-celled and do not bear spines at the periphery of cells, while the disc-shaped conidia of
<taxonomicName lsidName="S. deightonii" pageId="0" pageNumber="19" rank="species" species="deightonii">
<emphasis italics="true" pageId="0" pageNumber="19">S. deightonii</emphasis>
</taxonomicName>
are 8-celled and spiny. Based on contrasting morphological differences and significant statistical support from our molecular phylogeny,
<taxonomicName authorityName="Samarakoon, Phookamsak, Wanas., Chomnunti &amp; K. D. Hyde" authorityYear="2020" class="Dothideomycetes" family="Microthyriaceae" genus="Spegazzinia" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spegazzinia musae" order="Microthyriales" pageId="0" pageNumber="19" phylum="Ascomycota" rank="species" species="musae">
<emphasis italics="true" pageId="0" pageNumber="19">Spegazzinia musae</emphasis>
</taxonomicName>
is introduced as a new species.
</paragraph>
<caption doi="10.3897/mycokeys.70.52043.figure3" httpUri="https://binary.pensoft.net/fig/434239" pageId="0" pageNumber="19" start="Figure 3" startId="F3">
<paragraph pageId="0" pageNumber="19">
<emphasis bold="true" pageId="0" pageNumber="19">Figure 3.</emphasis>
<taxonomicName authorityName="Samarakoon, Phookamsak, Wanas., Chomnunti &amp; K. D. Hyde" authorityYear="2020" class="Dothideomycetes" family="Microthyriaceae" genus="Spegazzinia" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Spegazzinia musae" order="Microthyriales" pageId="0" pageNumber="19" phylum="Ascomycota" rank="species" species="musae">
<emphasis italics="true" pageId="0" pageNumber="19">Spegazzinia musae</emphasis>
</taxonomicName>
(MFLU 19-2907, holotype)
<emphasis bold="true" pageId="0" pageNumber="19">a-c</emphasis>
fungal colonies on host surface
<emphasis bold="true" pageId="0" pageNumber="19">d</emphasis>
mature conidia
<emphasis bold="true" pageId="0" pageNumber="19">e</emphasis>
conidiophore of
<normalizedToken originalValue="α">α</normalizedToken>
conidia with the mother cell
<emphasis bold="true" pageId="0" pageNumber="19">f, g</emphasis>
<normalizedToken originalValue="α">α</normalizedToken>
conidia
<emphasis bold="true" pageId="0" pageNumber="19">h-q</emphasis>
<normalizedToken originalValue="β">β</normalizedToken>
conidia
<emphasis bold="true" pageId="0" pageNumber="19">r</emphasis>
colony on PDA after 28 days. Scale bars: 200
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="19">a-c</emphasis>
), 20
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="19">d-g, j</emphasis>
), 10
<normalizedToken originalValue="μm">μm</normalizedToken>
(
<emphasis bold="true" pageId="0" pageNumber="19">h, i, k-q</emphasis>
).
</paragraph>
</caption>
</subSubSection>
</treatment>
</document>