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<document id="67414469BB1976CB23231A6EFC980841" ID-DOI="http://dx.doi.org/10.3897/mycokeys.50.32432" ID-GBIF-Dataset="059c89da-7ae5-4dbb-b876-bd1617110873" ID-PMC="PMC6477855" ID-Pensoft-Pub="1314-4049-50-1" ID-PubMed="31043855" ModsDocAuthor="" ModsDocDate="2019" ModsDocID="1314-4049-50-1" ModsDocOrigin="MycoKeys 50" ModsDocTitle="Solving the taxonomic identity of Pseudotomentellatristis s.l. (Thelephorales, Basidiomycota) a multi-gene phylogeny and taxonomic review, integrating ecological and geographical data" checkinTime="1555333405629" checkinUser="pensoft" docAuthor="Svantesson, Sten, Larsson, Karl-Henrik, Koljalg, Urmas, W. May, Tom, Patrik Cangren,, Henrik Nilsson, R. &amp; Larsson, Ellen" docDate="2019" docId="24D0E84BED12A8894B0F275737BB89B6" docLanguage="en" docName="MycoKeys 50: 1-77" docOrigin="MycoKeys 50" docSource="http://dx.doi.org/10.3897/mycokeys.50.32432" docTitle="Pseudotomentella media Svantesson &amp; Koljalg, sp. nov." docType="treatment" docVersion="5" lastPageNumber="27" masterDocId="BF413468CD59FFA53A5DFFC1FF9F272E" masterDocTitle="Solving the taxonomic identity of Pseudotomentellatristis s. l. (Thelephorales, Basidiomycota) - a multi-gene phylogeny and taxonomic review, integrating ecological and geographical data" masterLastPageNumber="77" masterPageNumber="1" pageNumber="25" updateTime="1732478795134" updateUser="ExternalLinkService">
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<mods:title id="A419E370A8E2F474155DBFB579525EA9">Solving the taxonomic identity of Pseudotomentellatristis s. l. (Thelephorales, Basidiomycota) - a multi-gene phylogeny and taxonomic review, integrating ecological and geographical data</mods:title>
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<mods:namePart id="FB9E3A030EE32471280E40A5453BB744">Svantesson, Sten</mods:namePart>
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<mods:namePart id="F664847EC92691C554C6625E3AE3F1AC">Larsson, Karl-Henrik</mods:namePart>
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<mods:namePart id="CB437C7113EFCF83C6A943467F945D53">Koljalg, Urmas</mods:namePart>
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<mods:namePart id="B87F3335F42EED871C0E8D9EDF3FB935">W. May, Tom</mods:namePart>
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<mods:namePart id="06AC2B8FC465F22411D8B8A7E9A48D74">Henrik Nilsson, R.</mods:namePart>
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<treatment id="24D0E84BED12A8894B0F275737BB89B6" ID-GBIF-Taxon="156201986" LSID="urn:lsid:plazi:treatment:24D0E84BED12A8894B0F275737BB89B6" httpUri="http://treatment.plazi.org/id/24D0E84BED12A8894B0F275737BB89B6" lastPageId="26" lastPageNumber="27" pageId="24" pageNumber="25" scope_order="Thelephorales" scope_phylum="Basidiomycota">
<subSubSection id="1341214C0DB94A78A32959327ADDB6A1" pageId="24" pageNumber="25" type="nomenclature">
<paragraph id="C5A64CAA0F627D389B2FC7DBA0613D16" pageId="24" pageNumber="25">
<taxonomicName id="502684D7A0B6834084B5DBC6E8C380C1" ID-CoL="4PDRM" LSID="MB829000" authority="Svantesson &amp; Koljalg" class="Agaricomycetes" family="Thelephoraceae" genus="Pseudotomentella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Pseudotomentella media" order="Thelephorales" pageId="24" pageNumber="25" phylum="Basidiomycota" rank="species" species="media">
Pseudotomentella media Svantesson &amp;
<normalizedToken id="080F1091EE25C28C86FA81DBD1674350" originalValue="Kõljalg">Koljalg</normalizedToken>
</taxonomicName>
<taxonomicNameLabel id="5423F8E2116E399F9CE66AFC2FDC9311" pageId="24" pageNumber="25">sp. nov.</taxonomicNameLabel>
Fig. 11
</paragraph>
</subSubSection>
<subSubSection id="B8A2ACC890E9C6CA029808B16BC78DFF" pageId="24" pageNumber="25" type="materials_examined">
<paragraph id="2CBE67DE2D51D05F9EB27AF92D0D79F6" pageId="24" pageNumber="25">Type.</paragraph>
<paragraph id="6E85DE1C6C50B119643E525BA61E656B" pageId="24" pageNumber="25">
ESTONIA. Valga:
<normalizedToken id="34A1A69E754E889BD73A005428115ACA" originalValue="Otepää">Otepaeae</normalizedToken>
, Trommi, 12 September 2012, U.
<normalizedToken id="DB712BC57FF4480E43FC75B7324E850D" originalValue="Kõljalg">Koljalg</normalizedToken>
(holotype: TU 115609!, GenBank Acc. No. ITS: MK290714).
</paragraph>
</subSubSection>
<subSubSection id="DE662EC26F946C723B04D9C98A0BC35E" pageId="24" pageNumber="25" type="unite sh">
<paragraph id="9DCF738FD13818F3898DD85960F575BB" pageId="24" pageNumber="25">UNITE SH.</paragraph>
<paragraph id="C7D5CA31A77EB3ED87B95FA01A4D7BB3" pageId="24" pageNumber="25">SH005336.07FU</paragraph>
</subSubSection>
<subSubSection id="70FCCCD92CDCA3B2A6ED3BD1F9F4290C" pageId="24" pageNumber="25" type="etymology">
<paragraph id="5B06AF19C9626D379CD4DA843D220B60" pageId="24" pageNumber="25">Etymology.</paragraph>
<paragraph id="89E473DF7A6D6235DDAEBD3D142AE254" pageId="24" pageNumber="25">
The name refers to the middling morphological characters of the species, relative to other species in the
<taxonomicName id="26B0F1B8D5B64CB4933DCC99CE0D4FC1" lsidName="P. tristis" pageId="24" pageNumber="25" rank="species" species="tristis">P. tristis</taxonomicName>
group.
</paragraph>
</subSubSection>
<subSubSection id="160723CABC917DB2A962C251898CB86C" lastPageId="25" lastPageNumber="26" pageId="24" pageNumber="25" type="description">
<paragraph id="CDCE82EEE9E612C8475C1427AA4CF53E" pageId="24" pageNumber="25">Description.</paragraph>
<paragraph id="7698BE3404CA548F85764FBFE07F4B28" pageId="24" pageNumber="25">Basidiomata annual, resupinate, membranaceous, effused to approximately ten centimetres in diameter. Mature parts continuous, with a rather firm, fibrous and compact, yet quite soft and elastic texture when dried. Hymenium smooth, but sometimes strongly undulating; brown with a reddish hue, both when fresh and when dried. Immature parts discontinuous, byssoid, with a cottony texture when dried. Subhymenium and hymenium of immature parts blue to greenish-blue when fresh and pale blue grey or blue grey to grey brown when dried. Subiculum well developed, loose, fibrous, pale brown to pale orange brown; forms the outer edge of basidiomata, extending noticeably beyond the hymenium.</paragraph>
<caption id="71A62BF67554D913B5D4990E72D48FFB" pageId="24" pageNumber="25">
<paragraph id="48888575D2713C4D04A1B9426EEA0F8D" pageId="24" pageNumber="25">
Figure 11. Morphological features of
<taxonomicName id="54B1D0AD538D6B5027956B9677882F83" lsidName="P. media" pageId="24" pageNumber="25" rank="species" species="media">P. media</taxonomicName>
, mounted in KOH and macroscopically. A, B basidiospores in frontal face (TU 115608) C in lateral face (TU 115608) D subicular hyphae (TU 115608) E younger basidiome (TU 115608) F mature basidiome (holotype).
</paragraph>
</caption>
<paragraph id="A426E851C4DBF9F02C3CE3B106FD2F70" pageId="24" pageNumber="25">Hyphal cords lacking, but loose bundles of subicular hyphae sometimes present.</paragraph>
<paragraph id="C5136D6BD2FB0DC8BC1164799FF45363" pageId="24" pageNumber="25">Hyphal system monomitic, clamp connections absent from all hyphae.</paragraph>
<paragraph id="E33362697B15CA8B220886B0B7B0EE42" pageId="24" pageNumber="25">
Subicular hyphae noticeably long and straight, thick-walled; forming a loose tissue. Individual hyphae (3.6-) 3.7-5.0 (-5.4)
<normalizedToken id="9D42D8FFCF8409E9F7E630C14BCE35EA" originalValue="μm">μm</normalizedToken>
wide, with a mean width of 4.1-4.6
<normalizedToken id="4976C30D7274D328FE542CF0112E2630" originalValue="μm">μm</normalizedToken>
; orange brown to brown in both KOH and water.
</paragraph>
<paragraph id="71BDBA05CAFBD63119B2BCB7C68AA369" pageId="24" pageNumber="25">
Subhymenial hyphae often somewhat sinuous, thin to thick-walled; forming a rather dense tissue. Individual hyphae (3.2-) 3.4-5.6 (-5.7)
<normalizedToken id="A205FA14251155F24A2BA57ECEF42648" originalValue="μm">μm</normalizedToken>
wide, with a mean width of 4.3-4.4
<normalizedToken id="F35C9A507A9B14A1693868D9FF28932E" originalValue="μm">μm</normalizedToken>
; pale brown, pale orange brown, pale greenish-brown or hyaline in KOH, blue green in the presence of air; orange green to brown in water, with strongly granular contents. Some subhymenial hyphae with a pink colour in water and an amyloid reaction.
</paragraph>
<paragraph id="774FEA626DDD72C77917DC7B54A5CD36" pageId="24" pageNumber="25">Encrustation granular, probably amyloid (hard to observe due to the colour); blackish in KOH, dark blue green in the presence of air; blackish in water; scattered in occurrence on the upper parts of subhymenial hyphae and on the lower parts of basidia.</paragraph>
<paragraph id="3A2FFC82CC90A93DD8DBA5EBAABE546D" pageId="24" pageNumber="25">
Basidia with four slightly curved sterigmata, occasionally two-sterigmate; clavate or sometimes narrowly clavate or clavopedunculate, thin-walled, with one-three slight constrictions. Dimensions: (57-) 58-84 (-87)
<normalizedToken id="E93F24FA224DB28FAC831142E3ABDB2C" originalValue="×">x</normalizedToken>
(8.8-) 9.6-11.8 (-12.5)
<normalizedToken id="4DCCCA8BD1E2A046EF335319205B95C0" originalValue="μm">μm</normalizedToken>
; mean dimensions: 74-77
<normalizedToken id="EA809A2C5A3D0438309FD3BFB6FB43BF" originalValue="×">x</normalizedToken>
10.2-10.7
<normalizedToken id="E2436FF2C1D27235F5AFF054EA340381" originalValue="μm">μm</normalizedToken>
. Sterigmata (9.4-) 9.5-11.4 (-11.7)
<normalizedToken id="B04DF36E8496B3D1F53246E317198132" originalValue="μm">μm</normalizedToken>
long, with a mean length of 10.0-10.8
<normalizedToken id="20AD9E5206A6F90A1E1B85016F9CF2B8" originalValue="μm">μm</normalizedToken>
. Colours and reactions the same as for the subhymenial hyphae, but in addition often with granular contents in KOH.
</paragraph>
<paragraph id="B79BAEF8163106B0D5FA0F21A1D87498" pageId="24" pageNumber="25">Cystidial organs lacking.</paragraph>
<paragraph id="D32EE0C6EE1271197A1A2B3BEEB69259" lastPageId="25" lastPageNumber="26" pageId="24" pageNumber="25">
Basidiospores in frontal face generally with a subcircular basic shape and an angular to nodulose or sometimes cross-shaped outline, covered in bi- or trifurcate, sometimes singularly attached, echinuli. A majority of the spores with three-six indistinct corners to distinct, square lobes; unlobed subellipsoid spores infrequently occurring, as well as abnormally large spores originating from two-sterigmate basidia. Frontal dimensions: (7.8-) 8.0-9.5
<normalizedToken id="F12264F94CAE235EAC1C828BDE00B59C" originalValue="×">x</normalizedToken>
(8.3-) 8.5-9.9 (-10.1)
<normalizedToken id="A0DB34FF2DA526E10B41CD872AE95730" originalValue="μm">μm</normalizedToken>
; mean dimensions: 8.9-9.3
<normalizedToken id="56C4D1A56BBE616D23800B8BF3B50DE6" originalValue="×">x</normalizedToken>
9.2-9.8
<normalizedToken id="1D0D568587541A44846506883E0047B5" originalValue="μm">μm</normalizedToken>
; Q-value: 0.9-1.0; mean Q-value: 1.0. Echinuli (0.8-) 0.9-1.6
<normalizedToken id="3AD790CB9A7BCD5D163164083F084678" originalValue="μm">μm</normalizedToken>
long, with a mean length of 1.1-1.2
<normalizedToken id="D2C220361B34C22315DE61E7024935F9" originalValue="μm">μm</normalizedToken>
. Lateral face ellipsoid, usually with evenly rounded edges, sometimes with one-three lobes. Lateral dimensions: (8.8-) 8.9-9.6
<normalizedToken id="ED69B0C3DE7FC8CBF9D2850344AF6A74" originalValue="×">x</normalizedToken>
(6.6-) 7.0-7.9
<normalizedToken id="D359073D74CEF21F0E17A05C5325F7D3" originalValue="μm">μm</normalizedToken>
; mean dimensions: 9.1-9.4
<normalizedToken id="C0E6B2AC97154DFA5E533C171AFE1AF8" originalValue="×">x</normalizedToken>
7.3-7.6
<normalizedToken id="F9BEE0483BD03350D6FA51CBD0A2DCAD" originalValue="μm">μm</normalizedToken>
; Q-value: 1.2-1.3;
<pageBreakToken id="6B5BB978BC08D87F976BC0EF862E88AE" pageId="25" pageNumber="26" start="start">mean</pageBreakToken>
Q-value: 1.2-1.3. Colour in KOH pale brown to brown or pale orange brown to orange brown, in the presence of air sometimes blue green; in water orange brown to brown; inamyloid.
</paragraph>
<paragraph id="BFFC4369DC00C9922523E5CDF74E122C" pageId="25" pageNumber="26">Chlamydospores lacking.</paragraph>
</subSubSection>
<subSubSection id="D7F16D4729F226C3E9873337D852332A" pageId="25" pageNumber="26" type="habitat">
<paragraph id="D2D6A7C2A71F8CC4FE8B6A0FA374C9E4" pageId="25" pageNumber="26">Habitat.</paragraph>
<paragraph id="53D2947335AD278BFE079A18A08CA990" pageId="25" pageNumber="26">
<taxonomicName id="726E7AF5A78EFCBCE2802207B882E566" lsidName="P. media" pageId="25" pageNumber="26" rank="species" species="media">P. media</taxonomicName>
has been found to form ectomycorrhiza with at least
<taxonomicName id="3DA88C83BC1EBC60A59105F56A9B1CC0" class="Magnoliopsida" family="Betulaceae" genus="Betula" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Betula pendula" order="Fagales" pageId="25" pageNumber="26" phylum="Tracheophyta" rank="species" species="pendula">Betula pendula</taxonomicName>
,
<taxonomicName id="92556FA0759C9C3286D2FC1D5253315A" class="Pinopsida" family="Pinaceae" genus="Larix" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Larix decidua" order="Pinales" pageId="25" pageNumber="26" phylum="Tracheophyta" rank="species" species="decidua">Larix decidua</taxonomicName>
and
<taxonomicName id="945BA15DED9C7AF724859E258B9B2B06" class="Pinopsida" family="Pinaceae" genus="Picea" higherTaxonomySource="CoL" kingdom="Plantae" lsidName="Picea glauca" order="Pinales" pageId="25" pageNumber="26" phylum="Tracheophyta" rank="species" species="glauca">Picea glauca</taxonomicName>
(
<bibRefCitation id="2D416AACE451C023B525968BB51F0866" author="Koljalg, U" journalOrPublisher="New Phytologist" pageId="61" pageNumber="62" pagination="1063 - 1068" title="UNITE: a database providing web-based methods for the molecular identification of ectomycorrhizal fungi." url="https://doi.org/10.1111/j.1469-8137.2005.01376.x" volume="166" year="2005">
<normalizedToken id="4C6FB5C9FD2EFBBE4AE7BDECE9B2F5C8" originalValue="Kõljalg">Koljalg</normalizedToken>
et al. 2005
</bibRefCitation>
,
<bibRefCitation id="128251026CA14958C181169393FD353C" author="Nilsson, RH" journalOrPublisher="MycoKeys" pageId="62" pageNumber="63" url="https://doi.org/10.1093/nar/gky1022" year="2019">Nilsson et al. 2019</bibRefCitation>
).
</paragraph>
</subSubSection>
<subSubSection id="1B6EE0049964C315A94E2C59617E596B" pageId="25" pageNumber="26" type="distribution">
<paragraph id="2291FBF5C2C8989A7BE48282548218DA" pageId="25" pageNumber="26">Distribution.</paragraph>
<paragraph id="2F58BC5DD4130B6F145F238E24E671A5" pageId="25" pageNumber="26">Basidiomata encountered in: Estonia. Soil or root tip samples confirm presence also in: Canada, Italy and Russia.</paragraph>
</subSubSection>
<subSubSection id="B34677A098298783FD43EADCBB0F135F" pageId="26" pageNumber="27" type="remarks">
<paragraph id="B7350EB5635786C3B2A5886A134A4041" pageId="26" pageNumber="27">
<pageBreakToken id="25B3EE27177DC9290FF42C42F1F69D4F" pageId="26" pageNumber="27" start="start">Remarks</pageBreakToken>
.
</paragraph>
<paragraph id="9F717938BF89E639F64D2EA2458E9675" pageId="26" pageNumber="27">
Within the
<taxonomicName id="86BFD4423F457CF2786849D253823B8F" lsidName="P. tristis" pageId="26" pageNumber="27" rank="species" species="tristis">P. tristis</taxonomicName>
group, the basidiomata of
<taxonomicName id="89AD388EE09BF9279323181ADB0F3D3D" lsidName="P. media" pageId="26" pageNumber="27" rank="species" species="media">P. media</taxonomicName>
can be recognised by their lack of hyphal cords and skeletal hyphae and their soft, yet rather firm and compact and
<normalizedToken id="9795EE52A27C5DC74C987F7247648ECA" originalValue="±">+/-</normalizedToken>
elastic texture after drying, bluish to greenish colour of immature parts, narrow subicular hyphae, brown mature hymenium, long, laterally wide, angular-nodulose spores and subhymenial hyphae that are of
<normalizedToken id="020346F8E5151D38CA033028970B6D75" originalValue="±">+/-</normalizedToken>
the same width as the subicular hyphae.
<taxonomicName id="719AC011B735D91C141B92A094BD7884" class="Agaricomycetes" family="Thelephoraceae" genus="Pseudotomentella" higherTaxonomySource="CoL" kingdom="Fungi" lsidName="Pseudotomentella pinophila" order="Thelephorales" pageId="26" pageNumber="27" phylum="Basidiomycota" rank="species" species="pinophila">Pseudotomentella pinophila</taxonomicName>
and
<taxonomicName id="CC6887BCAFFDDDAA2DA31584A5F5726F" lsidName="P. pluriloba" pageId="26" pageNumber="27" rank="species" species="pluriloba">P. pluriloba</taxonomicName>
can appear similar, but the spores of
<taxonomicName id="9D5E2E022D611486C95F9FFF58352FC5" lsidName="P. pinophila" pageId="26" pageNumber="27" rank="species" species="pinophila">P. pinophila</taxonomicName>
are laterally narrower and generally star-shaped, while
<taxonomicName id="5B37ABF93FF99F48ADE91954953596C3" lsidName="P. pluriloba" pageId="26" pageNumber="27" rank="species" species="pluriloba">P. pluriloba</taxonomicName>
has wider subicular hyphae, larger spores and subhymenial hyphae that are noticeably narrower than the subicular hyphae.
</paragraph>
</subSubSection>
<subSubSection id="822465873E668ED142FD1B47F4BA9BAB" pageId="26" pageNumber="27" type="additional specimens studied">
<paragraph id="52F1139EB5CC60A19C2909F8CB0283EE" pageId="26" pageNumber="27">Additional specimens studied.</paragraph>
<paragraph id="DD19E5495B3FB73DC9CD861997477E80" pageId="26" pageNumber="27">
ESTONIA. Valga:
<normalizedToken id="519A41C3A6C47F20AB59B469CB1E569A" originalValue="Otepää">Otepaeae</normalizedToken>
, Trommi, 12 September 2012, U.
<normalizedToken id="BEE933DA345739B93544A48F823DE7DC" originalValue="Kõljalg">Koljalg</normalizedToken>
(TU 115608*).
</paragraph>
</subSubSection>
</treatment>
</document>