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<mods:title id="C1FE2369BD3634D40947A4CCEA5BA2B5">Myxotrichum persicum sp. nov., and two new combinations in the genus Myxotrichum</mods:title>
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<mods:namePart id="177586DE5702745E63D9E8DA0EC163B1">Mehrabi, Mehdi</mods:namePart>
<mods:affiliation id="ADB7255A14CDE5783D340B13C45B9392">Department of Horticulture and Landscape Design, Shirvan Faculty of Agriculture, University of Bojnord, Bojnord, Iran</mods:affiliation>
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<mods:namePart id="7B316D842FAE4DD5DD0FBF042F0E8786">Asgari, Bita</mods:namePart>
<mods:affiliation id="45D1D93A786204D3FC344A28DEB9DDDC">Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran</mods:affiliation>
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<mods:namePart id="EBD209F6297B6C2DC01246F03C4D0180">Zare, Rasoul</mods:namePart>
<mods:affiliation id="59401B727A9CAA916960400C94CC7F0E">Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran</mods:affiliation>
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<mods:date id="0BE1EDA1BE5BB78CF6C648BC5AD7D0CC">2024</mods:date>
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<treatment id="03C88795547CFF80FF01FA1DFCA4F498" LSID="urn:lsid:plazi:treatment:03C88795547CFF80FF01FA1DFCA4F498" httpUri="http://treatment.plazi.org/id/03C88795547CFF80FF01FA1DFCA4F498" lastPageId="7" lastPageNumber="89" pageId="6" pageNumber="89">
<subSubSection id="C37B6508547CFF81FF01FA1DFCC6F163" box="[136,894,1076,1103]" pageId="6" pageNumber="89" type="nomenclature">
<paragraph id="8BDE3683547CFF81FF01FA1DFCC6F163" blockId="6.[136,894,1076,1103]" box="[136,894,1076,1103]" pageId="6" pageNumber="89">
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<taxonomicName id="4C614D00547CFF81FF01FA1DFD6CF162" authority="M. Mehrabi, Asgari &amp; Zare" authorityName="M. Mehrabi, Asgari &amp; Zare" box="[136,724,1076,1103]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="6" pageNumber="89" phylum="Ascomycota" rank="species" species="persicum" status="sp. nov.">
<emphasis id="B915EA91547CFF81FF01FA1DFE2EF163" bold="true" box="[136,406,1076,1103]" italics="true" pageId="6" pageNumber="89">Myxotrichum persicum</emphasis>
M. Mehrabi, Asgari &amp; Zare
</taxonomicName>
,
<taxonomicNameLabel id="A22657EA547CFF81FD56FA1CFC8DF162" box="[735,821,1077,1103]" pageId="6" pageNumber="89" rank="species">
<emphasis id="B915EA91547CFF81FD56FA1CFC96F163" box="[735,814,1077,1102]" italics="true" pageId="6" pageNumber="89">sp. nov</emphasis>
.
</taxonomicNameLabel>
<figureCitation id="135A2A06547CFF81FCB5FA1DFCC6F163" box="[828,894,1076,1102]" captionStart="FIGURE 2" captionStartId="8.[136,229,1499,1521]" captionTargetBox="[151,1436,190,1475]" captionTargetId="figure-21@8.[151,1436,190,1475]" captionTargetPageId="8" captionText="FIGURE 2. Myxotrichum persicum. a. Colonies morphology (surface and reverse) from left to right on PDA, PCA and CMA at 25 °C, after 4 weeks of incubation. b.Ascoma. c. Peridial hyphae. d, e. Appendages. f. Immature asci (arrow) and peridial hyphae. g. Ascospores. Scale bars: b, f=20 μm, ce, g=10 μm." figureDoi="http://doi.org/10.5281/zenodo.13215918" httpUri="https://zenodo.org/record/13215918/files/figure.png" pageId="6" pageNumber="89">Fig. 2</figureCitation>
</heading>
</paragraph>
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<subSubSection id="C37B6508547CFF81FF01FA75FED5F15F" box="[136,365,1116,1138]" pageId="6" pageNumber="89" type="description">
<paragraph id="8BDE3683547CFF81FF01FA75FED5F15F" blockId="6.[136,365,1116,1138]" box="[136,365,1116,1138]" pageId="6" pageNumber="89">MycoBank: MB851580</paragraph>
</subSubSection>
<subSubSection id="C37B6508547CFF81FF01FA89FC41F197" box="[136,1017,1184,1211]" pageId="6" pageNumber="89" type="etymology">
<paragraph id="8BDE3683547CFF81FF01FA89FC41F197" blockId="6.[136,1452,1184,2075]" box="[136,1017,1184,1211]" pageId="6" pageNumber="89">
Etymology:
<taxonomicName id="4C614D00547CFF81FE9AFA88FEC2F197" box="[275,378,1185,1210]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="6" pageNumber="89" phylum="Ascomycota" rank="species" species="persicum">
<emphasis id="B915EA91547CFF81FE9AFA88FEC2F197" box="[275,378,1185,1210]" italics="true" pageId="6" pageNumber="89">persicum</emphasis>
</taxonomicName>
of
<collectingCountry id="F3767613547CFF81FE16FA89FE5EF197" box="[415,486,1184,1210]" name="Iran" pageId="6" pageNumber="89">Persia</collectingCountry>
, present
<collectingCountry id="F3767613547CFF81FDC3FA88FDC2F196" box="[586,634,1185,1211]" name="Iran" pageId="6" pageNumber="89">Iran</collectingCountry>
, referring to the country of origin.
</paragraph>
</subSubSection>
<subSubSection id="C37B6508547CFF81FF34FAEDFDB6F0F7" pageId="6" pageNumber="89" type="description">
<paragraph id="8BDE3683547CFF81FF34FAEDFDB6F0F7" blockId="6.[136,1452,1184,2075]" pageId="6" pageNumber="89">Ascomata superficial, discrete, at first dull green to brown, then becoming nearly black, with a white centrum, more or less globose, 100200 μm diam incl. appendages, maturing in 4 weeks on PCA. Peridial hyphae dark brown, thick-walled, septate, sometimes constricted at the septum, 23.5 μm diam., smooth, branched and anastomosed to form a reticulum, ending in spine-like appendages. Appendages dark brown to nearly black, paling towards the apex, 2045 × 12.5 μm (av. = 37 × 1.7 μm, n = 10), straight or somewhat curved, usually unbranched, 12 septate at the lower part, smooth, with hyaline pointed or blunt end. Asci ovoid to pyriform, 8-spored, 79 × 68 μm (av. = 8.2 × 6.3 μm, n = 5), evanescent. Ascospores hyaline, smooth, ellipsoidal to fusiform, 34 × 22.5 μm (av. = 3.4 × 2 μm, n = 10). Asexual morph undetermined.</paragraph>
</subSubSection>
<subSubSection id="C37B6508547CFF81FF34FBCDFE30F3D7" pageId="6" pageNumber="89" type="biology_ecology">
<paragraph id="8BDE3683547CFF81FF34FBCDFE30F3D7" blockId="6.[136,1452,1184,2075]" pageId="6" pageNumber="89">
Culture characteristics (25 °C, 4 weeks): Vegetative mycelium consisting of hyaline, branched, septate, smooth-walled, 12 μm diam hyphae. Ascomatal initials appear as swollen side branches arising from aerial hyphae, which coil and form a compact mass. Colonies on PDA attaining
<quantity id="4C999B66547CFF81FC9FF805FCDDF36A" box="[790,869,1580,1607]" metricMagnitude="-2" metricUnit="m" metricValue="2.7" pageId="6" pageNumber="89" unit="mm" value="27.0">27 mm</quantity>
diam., flat, at first velvety, then becoming powdery, white, wrinkled, often deeply immersed into the agar; margins irregular; reverse orange (7) in center and buff (45) in margin. Colonies on PCA attaining
<quantity id="4C999B66547CFF81FD9EF85DFDDEF3A2" box="[535,614,1652,1679]" metricMagnitude="-2" metricUnit="m" metricValue="3.0" pageId="6" pageNumber="89" unit="mm" value="30.0">30 mm</quantity>
diam., flat, floccose, white; margins irregular; reverse of the same obverse colony color. Colonies on CMA attaining
<quantity id="4C999B66547CFF81FDE3F8B1FD04F39E" box="[618,700,1688,1715]" metricMagnitude="-2" metricUnit="m" metricValue="3.3" pageId="6" pageNumber="89" unit="mm" value="33.0">33 mm</quantity>
diam., flat, floccose, white, often deeply immersed into the agar; margins irregular; reverse of the same obverse colony color. Diffusible rose (1) to orange (7) pigments were produced in all examined media.
</paragraph>
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<subSubSection id="C37B6508547CFF81FF34F92DFE58F24A" pageId="6" pageNumber="89" type="materials_examined">
<paragraph id="8BDE3683547CFF81FF34F92DFE58F24A" blockId="6.[136,1452,1184,2075]" pageId="6" pageNumber="89">
<emphasis id="B915EA91547CFF81FF34F92DFF40F233" bold="true" box="[189,248,1796,1822]" pageId="6" pageNumber="89">Type</emphasis>
:—
<materialsCitation id="3B093CDE547CFF81FE93F92DFA1FF26F" collectingDate="2018-08-13" collectionCode="IRAN" collectorName="M. Mehrabi &amp; B. Asgari" country="Iran" location="Azna" pageId="6" pageNumber="89" specimenCode="IRAN 18352" specimenCount="1" stateProvince="Lorestan province" typeStatus="holotype">
<collectingCountry id="F3767613547CFF81FE93F92DFEDBF233" box="[282,355,1796,1822]" name="Iran" pageId="6" pageNumber="89">IRAN</collectingCountry>
,
<collectingRegion id="49A5F861547CFF81FEE6F92CFD86F233" box="[367,574,1796,1823]" country="Iran" name="Lorestan" pageId="6" pageNumber="89">Lorestan province</collectingRegion>
,
<location id="8EBE6058547CFF81FDC1F92DFD3EF233" LSID="urn:lsid:plazi:treatment:03C88795547CFF80FF01FA1DFCA4F498:8EBE6058547CFF81FDC1F92DFD3EF233" box="[584,646,1796,1822]" country="Iran" name="Azna" pageId="6" pageNumber="89" stateProvince="Lorestan province">Azna</location>
, 33˚2746.4″ N, 49˚2314.71″ E, seed endophyte of
<taxonomicName id="4C614D00547CFF81FB46F92DFA13F233" box="[1231,1451,1796,1822]" class="Liliopsida" family="Poaceae" genus="Aegilops" kingdom="Plantae" order="Poales" pageId="6" pageNumber="89" phylum="Tracheophyta" rank="species" species="triuncialis">
<emphasis id="B915EA91547CFF81FB46F92DFA13F233" box="[1231,1451,1796,1822]" italics="true" pageId="6" pageNumber="89">Aegilops triuncialis</emphasis>
</taxonomicName>
L.,
<date id="FFDF1043547CFF81FF27F901FED5F26F" box="[174,365,1832,1858]" pageId="6" pageNumber="89" value="2018-08-13">
<collectingDate id="EF9BE9AB547CFF81FF27F901FED5F26F" box="[174,365,1832,1858]" pageId="6" pageNumber="89" value="2018-08-13">August 13, 2018</collectingDate>
</date>
,
<collectorName id="26945355547CFF81FEF0F900FE47F26F" box="[377,511,1832,1859]" pageId="6" pageNumber="89">M. Mehrabi</collectorName>
&amp;
<collectorName id="26945355547CFF81FDACF900FD28F26F" box="[549,656,1832,1859]" pageId="6" pageNumber="89">B. Asgari</collectorName>
(
<emphasis id="B915EA91547CFF81FD28F901FCB0F26F" bold="true" box="[673,776,1832,1858]" pageId="6" pageNumber="89">
<typeStatus id="54DA8821547CFF81FD28F901FCB0F26F" box="[673,776,1832,1858]" pageId="6" pageNumber="89" type="holotype">holotype</typeStatus>
</emphasis>
<specimenCode id="DBC79EF8547CFF81FC99F901FC1AF26E" box="[784,930,1832,1859]" pageId="6" pageNumber="89">IRAN 18352</specimenCode>
F, preserved in a metabolically inactive state)
</materialsCitation>
;
<emphasis id="B915EA91547CFF81FF01F965FF67F24B" bold="true" box="[136,223,1868,1894]" pageId="6" pageNumber="89">ex-type</emphasis>
culture,
<collectingCountry id="F3767613547CFF81FECBF965FE3EF24B" box="[322,390,1868,1894]" name="Iran" pageId="6" pageNumber="89">IRAN</collectingCountry>
3775C.
</paragraph>
</subSubSection>
<subSubSection id="C37B6508547CFF80FF34F958FCA4F498" lastPageId="7" lastPageNumber="90" pageId="6" pageNumber="89" type="discussion">
<paragraph id="8BDE3683547CFF80FF34F958FCA4F498" blockId="6.[136,1452,1184,2075]" lastBlockId="7.[136,1452,158,437]" lastPageId="7" lastPageNumber="90" pageId="6" pageNumber="89">
Notes: Attempts to stimulate sporulation of our strain on various culture media including CMA, OA, PCA, PDA and SNA were unsuccessful, and only a few ascomata were formed on PCA. Morphologically,
<taxonomicName id="4C614D00547CFF81FB4FF9BCFAECF283" box="[1222,1364,1941,1966]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="6" pageNumber="89" phylum="Ascomycota" rank="species" species="persicum">
<emphasis id="B915EA91547CFF81FB4FF9BCFAECF283" box="[1222,1364,1941,1966]" italics="true" pageId="6" pageNumber="89">M. persicum</emphasis>
</taxonomicName>
closely resembles
<taxonomicName id="4C614D00547CFF81FE89F990FE33F2FF" box="[256,395,1976,2002]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="6" pageNumber="89" phylum="Ascomycota" rank="species" species="deflexum">
<emphasis id="B915EA91547CFF81FE89F990FE33F2FF" box="[256,395,1976,2002]" italics="true" pageId="6" pageNumber="89">M. deflexum</emphasis>
</taxonomicName>
and
<taxonomicName id="4C614D00547CFF81FE4AF990FC39F2FE" authority="Matsush. However" authorityName="Matsush. However" box="[451,897,1976,2003]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="6" pageNumber="89" phylum="Ascomycota" rank="species" species="dendromorphum">
<emphasis id="B915EA91547CFF81FE4AF990FD1CF2FF" box="[451,676,1976,2002]" italics="true" pageId="6" pageNumber="89">M. dendromorphum</emphasis>
Matsush. However
</taxonomicName>
, this new species is primarily distinguished from similar species by the characteristics of ascomata appendages and ascospores. In comparison to
<taxonomicName id="4C614D00547CFF81FB4BF9F4FF74FD36" authority="(Currah 1985)" baseAuthorityName="Currah" baseAuthorityYear="1985" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="6" pageNumber="89" phylum="Ascomycota" rank="species" species="deflexum">
<emphasis id="B915EA91547CFF81FB4BF9F4FAF5F2DB" box="[1218,1357,2012,2038]" italics="true" pageId="6" pageNumber="89">M. deflexum</emphasis>
(
<bibRefCitation id="EFF04B72547CFF81FAD5F9F5FF7BFD36" author="Currah, R. S." pageId="6" pageNumber="89" pagination="1 - 216" refId="ref9082" refString="Currah, R. S. (1985) Taxonomy of the Onygenales: Arthrodermataceae, Gymnoascaceae, Myxotrichaceae and Onygenaceae. Mycotaxon 24: 1 - 216." type="journal article" year="1985">Currah 1985</bibRefCitation>
)
</taxonomicName>
, which infrequently produces alternate arthroconidia (malbranchea-like),
<taxonomicName id="4C614D00547CFF81FC75F628FB3FFD37" box="[1020,1159,2049,2074]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="6" pageNumber="89" phylum="Ascomycota" rank="species" species="persicum">
<emphasis id="B915EA91547CFF81FC75F628FB3FFD37" box="[1020,1159,2049,2074]" italics="true" pageId="6" pageNumber="89">M. persicum</emphasis>
</taxonomicName>
exhibits smaller ascomata (100200 μm vs. 200450 μm). Furthermore,
<taxonomicName id="4C614D00547DFF80FD1FFEB6FC9CF595" box="[662,804,159,184]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="7" pageNumber="90" phylum="Ascomycota" rank="species" species="persicum">
<emphasis id="B915EA91547DFF80FD1FFEB6FC9CF595" box="[662,804,159,184]" italics="true" pageId="7" pageNumber="90">M. persicum</emphasis>
</taxonomicName>
has shorter (2045 μm vs. up to 200 μm), less branched appendages with deflexed lateral branches, and ellipsoidal, smooth ascospores. Phylogenetic analyses (
<figureCitation id="135A2A06547DFF80FAAEFEEBFAD1F5F1" box="[1319,1385,194,220]" captionStart="FIGURE 1" captionStartId="7.[136,229,1898,1920]" captionTargetBox="[158,1429,465,1863]" captionTargetId="figure-142@7.[151,1436,458,1870]" captionTargetPageId="7" captionText="FIGURE 1. Maximum likelihood phylogenetic tree inferred from the combined ITS, LSU, MCM7 and RPB1 sequence alignment of 55 strains of the Myxotrichaceae and Amorphothecaceae. Maximum likelihood bootstrap supports (≥70%) and Bayesian posterior probabilities support (≥0.9) are given above or below the branches, respectively. Dashes replace non-significant values. The branches with full statistical support (ML = 100%; PP = 1.0) are thickened. The scale bar shows the expected number of changes per site. The tree is rooted to Hyphodiscus hyaloscyphoides (NBRC 104869) and Venturiocistella japonica (NBRC 106633)." figureDoi="http://doi.org/10.5281/zenodo.13215916" httpUri="https://zenodo.org/record/13215916/files/figure.png" pageId="7" pageNumber="90">Fig. 1</figureCitation>
) also support the distinction of
<taxonomicName id="4C614D00547DFF80FE2DFECEFD97F42D" box="[420,559,231,256]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="7" pageNumber="90" phylum="Ascomycota" rank="species" species="persicum">
<emphasis id="B915EA91547DFF80FE2DFECEFD97F42D" box="[420,559,231,256]" italics="true" pageId="7" pageNumber="90">M. persicum</emphasis>
</taxonomicName>
from
<taxonomicName id="4C614D00547DFF80FDF8FECEFD43F42D" baseAuthorityName="Currah" baseAuthorityYear="1985" box="[625,763,230,256]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="7" pageNumber="90" phylum="Ascomycota" rank="species" species="deflexum">
<emphasis id="B915EA91547DFF80FDF8FECEFD43F42D" box="[625,763,230,256]" italics="true" pageId="7" pageNumber="90">M. deflexum</emphasis>
</taxonomicName>
. Base pair differences further highlight distinctions, revealing 2% (545/554) and 6% (472/502) differences in LSU and ITS regions, respectively, following the guidelines of
<bibRefCitation id="EFF04B72547DFF80FADEFF22FEFFF465" author="Jeewon, R. &amp; Hyde, K. D." pageId="7" pageNumber="90" pagination="1669 - 1677" refId="ref9548" refString="Jeewon, R. &amp; Hyde, K. D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669 - 1677. https: // doi. org / 10.5943 / mycosphere / 7 / 11 / 4" type="journal article" year="2016">Jeewon and Hyde (2016)</bibRefCitation>
.
<taxonomicName id="4C614D00547DFF80FED8FF07FC3EF464" authority="(Matsushima 1995)" baseAuthorityName="Matsushima" baseAuthorityYear="1995" box="[337,902,302,329]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="7" pageNumber="90" phylum="Ascomycota" rank="species" species="dendromorphum">
<emphasis id="B915EA91547DFF80FED8FF07FD1FF465" box="[337,679,302,328]" italics="true" pageId="7" pageNumber="90">Myxotrichum dendromorphum</emphasis>
(
<bibRefCitation id="EFF04B72547DFF80FD3DFF07FCC6F464" author="Matsushima, T." box="[692,894,302,329]" pageId="7" pageNumber="90" pagination="1 - 44" refId="ref10275" refString="Matsushima, T. (1995) Matsushima Mycological Memoirs No. 8. Kobe, Japan. pp. 1 - 44." type="book chapter" year="1995">Matsushima 1995</bibRefCitation>
)
</taxonomicName>
also resembles
<taxonomicName id="4C614D00547DFF80FBBFFF06FB79F465" box="[1078,1217,303,328]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="7" pageNumber="90" phylum="Ascomycota" rank="species" species="persicum">
<emphasis id="B915EA91547DFF80FBBFFF06FB79F465" box="[1078,1217,303,328]" italics="true" pageId="7" pageNumber="90">M. persicum</emphasis>
</taxonomicName>
in ascomata size and peridial hyphae characteristics, but it can be differentiated by longer (40100 μm), tree-like branching appendages and larger (45.5 × 23 μm), and longitudinally striate ascospores. No sequence data are available for
<taxonomicName id="4C614D00547DFF80FB44FF5EFA14F4BD" baseAuthorityName="Matsushima" baseAuthorityYear="1995" box="[1229,1452,374,400]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="7" pageNumber="90" phylum="Ascomycota" rank="species" species="dendromorphum">
<emphasis id="B915EA91547DFF80FB44FF5EFA14F4BD" box="[1229,1452,374,400]" italics="true" pageId="7" pageNumber="90">M. dendromorphum</emphasis>
</taxonomicName>
to compare its phylogenetic relationship with
<taxonomicName id="4C614D00547DFF80FD00FFB2FCAEF499" box="[649,790,411,436]" class="Leotiomycetes" family="Amorphothecaceae" genus="Myxotrichum" kingdom="Fungi" order="Erysiphales" pageId="7" pageNumber="90" phylum="Ascomycota" rank="species" species="persicum">
<emphasis id="B915EA91547DFF80FD00FFB2FCAEF499" box="[649,790,411,436]" italics="true" pageId="7" pageNumber="90">M. persicum</emphasis>
</taxonomicName>
.
</paragraph>
</subSubSection>
</treatment>
</document>